Endophytic bacillus velezensis and application thereof in prevention and treatment of plant diseases

Abstract

The present application belongs to the field of agricultural microorganism technology, and particularly relates to a plant endophytic bacillus velezensis and application thereof in preventing and treating plant diseases. Bacillus velezensis The plant endophytic bacillus velezensis is BV17, is classified and named as bacillus velezensis, has a preservation number of CGMCC No. 37617, and is preserved in the China General Microbiological Culture Collection Center on February 2, 2026. The bacillus velezensis BV17 provided in the present application has a broad-spectrum inhibitory activity on pathogenic fungi, oomycetes and bacteria. Meanwhile, through pot experiment and field effect verification, the bacillus velezensis BV17 can maintain a good control effect under different environmental conditions, indicating that it has strong environmental adaptability and is suitable for popularization and application in actual agricultural production. Through application of the strain provided in the present application for disease prevention and control, the use amount of chemical pesticides can be effectively reduced, the environmental pollution risk is reduced, and the requirements of green agriculture and sustainable development are met.

Description

Technical Field

[0001] This invention belongs to the field of agricultural microbial technology, and specifically relates to a plant endophytic Bacillus belye and its application in the prevention and control of plant diseases. Background Technology

[0002] Soil-borne diseases are a significant group of diseases restricting sustainable agricultural production globally. Their pathogens are primarily fungi, bacteria, and oomycetes, which can survive in the soil medium for extended periods, infecting crop roots, root collars, and other underground tissues. Common soil-borne diseases include Fusarium oxysporum, Rhizoctonia solani, Pythium, and Phytophthora, causing wilt, root rot, and damping-off. These diseases are characterized by long incubation periods, rapid spread, wide distribution, and high recurrence rates, making them difficult to eradicate completely once they occur, and causing irreversible and severe damage to crop yield and quality.

[0003] Current soil-borne disease control systems encompass three main pathways: chemical control, optimized agricultural cultivation management, and biological control. While chemical pesticides can rapidly suppress pathogen populations and curb disease spread in the short term, long-term improper application can induce drug-resistant mutations in pathogens. Furthermore, it disrupts the original microecological balance of the soil, leading to a series of risks such as excessive pesticide residues, non-target biotoxicity, and environmental pollution, failing to meet the safety requirements of modern green and high-quality agricultural development. Therefore, developing efficient, low-risk, and environmentally friendly biological control technologies has become a core development direction in this field.

[0004] Endophytic microorganisms, capable of long-term colonization and stable symbiotic relationships within host plant tissues, represent a current hot topic in biocontrol resource development. Compared to biocontrol microorganisms that function only in the rhizosphere or on the plant surface, endophytic bacteria can colonize internal tissues such as roots, stems, and leaves, making them less susceptible to soil environmental fluctuations, rainfall erosion, and competition from rhizosphere microorganisms. This results in longer-lasting biocontrol effects and superior field stability. Endophytic biocontrol strains can not only directly inhibit pathogen proliferation by secreting antimicrobial metabolites, but also synergistically enhance crop resistance to soil-borne diseases through multiple pathways, including seizing ecological niches within the plant, competing for nutrients, and inducing systemic resistance in the host. This provides a new solution for the green control of soil-borne diseases.

[0005] Bacillus belyes is a typical beneficial group within the Bacillus genus, widely distributed in soil, plant rhizosphere, and tissues. It can produce resistant spores, exhibits strong environmental adaptability, and demonstrates good stability in formulation processing. It has been listed as a safe microbial group exempt from toxicological testing by the Ministry of Agriculture and Rural Affairs of my country. Existing studies have confirmed that Bacillus belyes can exert significant inhibitory effects on various soil-borne pathogenic fungi and bacteria through multiple mechanisms of action, including secreting lipopeptides and polyketides as antimicrobial substances, competing for nutrients and ecological niches, inducing systemic resistance in the host, and secreting auxins, phosphate solubilizing, potassium solubilizing, and growth-promoting agents. It possesses extremely high application potential in the field of biological control of plant diseases.

[0006] However, due to differences in strain origin, genetic background, and functional trait differentiation, different Bacillus berleis strains exhibit significant heterogeneity in plant endophytic colonization ability, antibacterial activity intensity, inhibitory spectrum range, metabolite composition, and field application stability. Currently, some publicly available strains still have shortcomings in practical applications, such as large fluctuations in control efficacy, insufficient inhibitory activity against specific soil-borne pathogens, and poor host adaptability, failing to meet the urgent agricultural demand for highly efficient, stable, and widely adaptable biocontrol agents.

[0007] Therefore, screening out new strains of Bacillus belyss that possess strong endogenous colonization ability, broad-spectrum and highly efficient inhibitory activity against soil-borne diseases, and can stably survive and continuously function in plants and soil environments, is of great practical significance and application value for improving the efficiency of biological control of soil-borne diseases, reducing the input of chemical pesticides, and promoting the green and sustainable development of agriculture. Summary of the Invention

[0008] This invention provides a strain of *Bacillus belye*, an endophytic plant fungus, with broad-spectrum antibacterial activity obtained through screening. This strain exhibits inhibitory effects against various plant pathogenic fungi and oomycetes. Furthermore, this strain possesses the ability to colonize within plant root tissues, allowing it to persist within the plant and exert its disease-suppressing effect, thereby further enhancing its control over soil-borne diseases and improving the persistence and stability of biological control.

[0009] In a first aspect, the present invention provides a plant endophytic Bacillus belyesii, wherein the plant endophytic Bacillus belyesii is BV17 and is classified as Bacillus belyesii. Bacillus velezensis The specimen, with accession number CGMCC No.37617, was deposited at the China General Microbiological Culture Collection Center on February 2, 2026.

[0010] Secondly, the present invention also provides a microbial bactericide containing the plant endophytic Bacillus belye described in the first aspect.

[0011] In some embodiments, the microbial bactericide further includes a carrier. The carrier can be a solid carrier or a liquid carrier. The solid carrier can be a mineral material or a biological material; the mineral material can be at least one selected from peat moss, clay, talc, kaolin, montmorillonite, white carbon, zeolite, silica, and diatomaceous earth; the biological material can be at least one selected from various crop straws, pine shells, rice straw, peanut shells, corn flour, soybean flour, starch, peat moss, and animal manure; the liquid carrier can be water.

[0012] In this invention, the microbial agent can be in various formulations, such as liquid, emulsion, suspension, powder, granules, wettable powder, or water-dispersible granules. Depending on the requirements, surfactants (such as Tween 20, Tween 80, etc.), binders, stabilizers (such as antioxidants), pH adjusters, etc., may also be added to the agent.

[0013] Thirdly, the present invention also provides the application of the plant endophytic Bacillus belye described in the first aspect or the microbial bactericide described in the second aspect, wherein the application is any one of the following: A1) Inhibits the growth of plant pathogens; A2) Prepare products that inhibit plant pathogens; A3) Prevention and control of plant diseases caused by plant pathogens; A4) Prepare products for preventing and treating plant diseases caused by plant pathogens.

[0014] In some embodiments, the plant pathogens include pathogenic fungi, pathogenic oomycetes, and pathogenic bacteria.

[0015] In some embodiments, the pathogenic fungus is selected from Fusarium graminearum (Fusarium graminearum). Fusarium gramineae Fusarium oxysporum ( Fusarium oxysporum Fusarium solani () Fusarium solani ), Fusarium graminearum ( Fusarium pseudograsses Verticillium dahliae Verticillium dahliae Rhizoctonia solani ( ), Rhizoctonia solani Rhizoctonia nightshade ) and gray mold ( Botrytis cinerea One or more of the following.

[0016] In some embodiments, the pathogenic oomycete is selected from *Pythium tertrum* (…). The last Pythium ), Phytophthora capsici ( Phytophthora capsicum ), Phytophthora ( Phytophthora cactorum ), Phytophthora parasiticum ( Phytophthora parasitic ), soybean phytotoxicum ( Phytophthora sojae One or more of the following.

[0017] In some embodiments, the pathogenic bacteria are selected from *Pseudomonas syringae* tomato pathogenic strain (… Pseudomonas syringes pv. tomato Kiwi fruit canker pathogen ( Pseudomonas syringae pv. actinidae ) and Ralstonia solanacearum ( Ralstonia solanacearum One or more of the following.

[0018] In some embodiments, the plant is a monocotyledonous plant or a dicotyledonous plant. The monocotyledonous plant can be wheat, rice, corn, sugarcane, etc., and the dicotyledonous plant can be soybean, potato, alfalfa, tobacco, tomato, cucumber, apple, etc. Preferably, the plant is soybean or alfalfa.

[0019] Fourthly, the present invention also provides a method for preventing and controlling plant diseases caused by plant pathogens, the method comprising treating plant tissues with the plant endophytic Bacillus belye described in the first aspect of the present invention or the microbial fungicide described in the second aspect of the present invention.

[0020] In some embodiments, the plant disease is caused by at least one pathogen described in the third aspect of the invention.

[0021] Preservation Information Classification and nomenclature: Bacillus belesiensis Bacillus velezensis Accession number: CGMCC No. 37617 Deposit date: February 2, 2026 Preservation Institution: China General Microbiological Culture Collection Center (CGMCC) Address of the depository: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.

[0022] Compared with the prior art, the present invention has the following technical effects.

[0023] 1) The Bacillus belye BV17 strain provided by this invention exhibits broad-spectrum inhibitory activity against pathogenic fungi, oomycetes, and bacteria, and can significantly inhibit plant pathogenic microorganisms through multiple mechanisms of action. Simultaneously, this strain has the ability to colonize within plant root tissues, allowing it to persist within the plant and exert its disease-suppressing effect, thereby further enhancing its control over soil-borne diseases and improving the persistence and stability of biological control.

[0024] 2) The efficacy of this invention has been verified through pot experiments and field tests. Bacillus belye BV17 maintains good control effects under different environmental conditions, indicating its strong environmental adaptability and suitability for widespread application in actual agricultural production. By applying the strain described in this invention for disease control, the use of chemical pesticides can be effectively reduced, lowering the risk of environmental pollution and meeting the requirements of green agriculture and sustainable development. Attached Figure Description

[0025] Figure 1 The colony morphology of Bacillus belyssus BV17.

[0026] Figure 2 This shows the inhibitory effect of Bacillus belyssus BV17 on pathogenic fungi. From left to right: Fusarium graminearum (…). Fusarium gramineae Fusarium oxysporum ( Fusarium oxysporum Fusarium solani () Fusarium nightshade ), Fusarium graminearum ( Fusarium pseudogramineum Verticillium dahliae Verticillium wilt dahlias Rhizoctonia solani ( ), Rhizoctonia solani Rhizoctonia solani ) and gray mold ( Botrytis cinerea ).

[0027] Figure 3 This shows the inhibitory effect of Bacillus belyssus BV17 on pathogenic oomycetes. From left to right, it represents Phytophthora infestans (…). Phytophthora cactorum ), soybean phytotoxicum ( Phytophthora sojae ) and ultimate pyrophyllium ( Pythium last ).

[0028] Figure 4 This image shows the inhibitory effect of Bacillus belye BV17 on pathogenic bacteria. From left to right: *Pseudomonas syringae* pathogenic strain in tomato (…). Pseudomonas syringae pv. tomato Kiwi fruit canker pathogen ( Pseudomonas syringes pv. actinidae ) and Ralstonia solanacearum ( Ralstonia solanacearum ).

[0029] Figure 5 The results show the effects of Bacillus belyssus BV17 bacterial suspension on the growth of soybeans and alfalfa. Figures AC and DE show the effects on soybean growth and alfalfa growth, respectively.

[0030] Figure 6 The colonization of Bacillus belye BV17 in different parts of alfalfa.

[0031] Figure 7The effect of Bacillus vesicles BV17 bacterial suspension on Fusarium graminearum ( Fusarium gramineae The control effect on soybean plants infected with the virus.

[0032] Figure 8 The effect of Bacillus vesiculosus BV17 bacterial suspension on the pathogenic oomycete Phytophthora ( Phytophthora cactorum The control effect on alfalfa plants infected with the virus.

[0033] Figure 9 The pathogenic fungus Fusarium solanum ( Fusarium solani The control effect on alfalfa plants infected with the virus.

[0034] Figure 10 Fusarium oxysporum ( Fusarium oxysporum The control effect on alfalfa plants infected with the virus.

[0035] Figure 11 Rhizoctonia solani ( Rhizoctonia solani The control effect on alfalfa plants infected with the virus. Detailed Implementation

[0036] The present invention will be further illustrated below with reference to specific embodiments. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Experimental methods in the following embodiments that do not specify specific conditions are generally performed under conventional conditions, conditions described in a laboratory manual, or conditions recommended by the manufacturer.

[0037] Example 1

[0038] Strain BV17 was isolated and screened from alfalfa roots. Sequencing was performed using the universal 16S primers 27F: 5'-AGAGTTTGATCMTGGCTCAG-3' (SEQ ID NO.1) and 1492R: 5'-TACGGYTACCTTGTTACGACTT-3' (SEQ ID NO.2), and the 16S sequence results (SEQ ID NO.3) were compared. The results showed that strain BV17 had a sequence similarity of over 99% with *Bacillus belyssiensis*, indicating a close phylogenetic relationship between the two strains and thus verifying their taxonomic position. Based on the sequencing results, it was identified as *Bacillus belyssiensis* (…). Bacillus from Velez ).

[0039] The strain was deposited on February 2, 2026, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37617. The deposit address is: Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing.

[0040] Example 2

[0041] Bacillus belye BV17 was inoculated onto LB solid medium and incubated at 37°C for 1 day. Figure 1 As shown, the colonies were observed to be nearly round, viscous, milky white, with a moist and smooth surface and irregular edges; the Gram staining result was positive.

[0042] Example 3

[0043] The inhibitory activity of strain BV17 against pathogenic fungi, oomycetes, and bacteria was determined using the plate confrontation method. The selected pathogenic fungus was *Fusarium graminearum* (…). Fusarium gramineae Fusarium oxysporum ( Fusarium oxysporum Fusarium solani () Fusarium solani ), Fusarium graminearum ( Fusarium pseudogramineum Verticillium dahliae Verticillium dahliae Rhizoctonia solani ( ), Rhizoctonia solani Rhizoctonia solani ) and gray mold ( Botrytis gray ); Pathogenic oomycetes: Phytophthora ( Phytophthora cactorum ), soybean phytotoxicum ( Phytophthora sojae ) and ultimate pyrophyllium ( The last Pythium ); Pathogenic bacteria: *Pseudomonas syringae* tomato pathogenic strain ( Pseudomonas syringes pv. tomato Kiwi fruit canker pathogen ( Pseudomonas syringae pv. actinidae ) and Ralstonia solanacearum ( Ralstonia solanacearum ).

[0044] The method for detecting pathogenic fungi and oomycetes is as follows: Add 20 mL of PDA / V8 medium to each 90 mm diameter petri dish. Use a 6 mm diameter punch to create filter paper discs, stacking two layers together and attaching them around the perimeter of the plate. Add 20 μL LOD to each disc. 600 The control group was prepared with a 1.0 g / L Bacillus berberis BV17 bacterial suspension. An equal volume of sterile water was added to the control group, and a 6 mm diameter pathogen block was inoculated in the center of the plate.

[0045] The method for identifying pathogenic bacteria is as follows: Use 90 mm diameter petri dishes, adding 20 mL of LB medium to each dish. Take 200 μL of OD... 600 For a 1.0 μL pathogenic bacteria suspension, a plate was prepared. Filter paper discs were punched out using a 6 mm diameter punch, stacked in a single layer, and then coated onto a plate. A 20 μL LOD drop was added to each disc. 600 The bacterial suspension of Bacillus vesiculosus BV17 was 1.0.

[0046] like Figure 2-4 As shown, Bacillus belye BV17 on culture medium is effective against pathogenic fungi (such as... Figure 2 ), oomycetes (such as Figure 3 ), bacteria (such as Figure 4The growth of all bacteria was inhibited, indicating that the strain has broad-spectrum resistance to the pathogen.

[0047] Example 4

[0048] Effects of Bacillus vesicularis BV17 bacterial suspension on plant growth. Bacterial suspension was prepared by sowing 10 alfalfa (Zhongmu No. 1) or soybean (Hefeng 47) seeds. Two treatment groups were established: a bacterial suspension group and a control group. Each treatment was irrigated with 50 mL of bacterial suspension, while the control group received water.

[0049] The results are as follows Figure 5 As shown, irrigation with Bacillus venetum BV17 bacterial solution had no significant adverse effects on the survival rate and fresh weight of soybeans and alfalfa.

[0050] Example 5

[0051] First, Bacillus berleis BV17 was induced to produce a rifampicin-resistant mutant strain, and then the colonization of Bacillus berleis BV17 in different parts of alfalfa was detected.

[0052] Bacillus belye BV17 was inoculated into LB medium and cultured at 220 rpm for 24 h at 37°C. 100 μL of the bacterial culture was spread onto LB agar plates containing 5 μg / mL rifampicin and cultured at 37°C for 3 days. The resulting mutant strain was picked and inoculated into LB medium containing 5 μg / mL rifampicin and cultured at 220 rpm for 24 h at 37°C. 100 μL of the bacterial culture was then spread onto LB agar plates containing 10 μg / mL rifampicin and cultured at 37°C for 3 days. This process was repeated to induce rifampicin resistance in the tested endophytic bacteria on LB agar plates containing 5, 10, 20, 40, and 50 μg / mL rifampicin until a strain capable of normal growth on LB agar plates containing 50 μg / mL rifampicin was selected. The mutant strain was subcultured three times on LB agar plates containing 50 μg / mL rifampicin, and the resistant mutant strain was purified by streaking on LB agar plates containing 50 μg / mL rifampicin. After being transferred to a plate without rifampicin and cultured for 3 generations, the plate was then transferred back to a plate containing 50 μg / ml rifampicin to test the stability of the resistance.

[0053] Ten sterilized alfalfa seeds were sown into sterilized vermiculite and irrigated with 50 mL of BV17 bacterial solution with a resistance tag. Eight days after root irrigation, samples were taken: 0.6 g each of roots, stems, and leaves were cut and surface-sterilized by soaking in 70% alcohol for 30 seconds, soaking in 2% sodium hypochlorite for 1 minute, and rinsing three times with sterile water. The samples were then ground and mixed in a mortar with 6 mL of sterile water. The supernatant was allowed to settle for 10 minutes and then diluted 10-fold to four concentrations (10-fold, 100-fold, 1000-fold, and 10000-fold, respectively). 0.1 mL of each concentration was spread onto LB agar plates containing 50 μg / mL rifampicin, with three replicates per concentration. The plates were incubated at 28°C for 2 days, and the colony count was recorded. The colony count per gram of fresh alfalfa tissue was calculated to evaluate colonization ability.

[0054] The results are as follows Figure 6 As shown, BV17 can colonize the roots, stems, and leaves of plants, but the highest number of colonies are found in the roots.

[0055] Example 6

[0056] Bacillus belyi BV17 can control Fusarium graminearum on soybeans. Fusarium graminearum Diseases caused by ).

[0057] The pathogen Fusarium graminearum ( Fusarium graminearum After the colonies have fully grown on 90mm plates, cut them into small pieces and mix them evenly into vermiculite. Inoculate two dishes of pathogens into each pot and sow 10 soybean seeds. Apply OD fertilizer on the day of inoculation and the fourth day after inoculation. 600 The solution was 1.0 BV17 bacterial solution. 50 mL of bacterial solution was poured into each pot, and the results were collected after 14 days.

[0058] The results are as follows Figure 7 As shown, the survival rate of soybean plants treated with Bacillus vesicles BV17 was significantly higher than that of the control plants, and their fresh weight was also significantly different from that of the control plants, indicating that Bacillus vesicles BV17 can effectively control soybean plant diseases caused by Fusarium graminearum.

[0059] Example 7

[0060] Phytophthora infestans ( Phytophthora cactorum ), pathogenic fungus Fusarium solanum ( Fusarium solani Fusarium oxysporum ( Fusarium oxysporum ) and Rhizoctonia solani ( Rhizoctonia solani After the colonies have fully grown on 90mm plates, cut them into small pieces and mix them evenly into vermiculite. Inoculate two dishes of pathogens into each pot and sow 10 alfalfa seeds. Apply OD fertilizer on the day of inoculation and on the fourth day after inoculation. 600Use a 1.0 BV17 bacterial solution and pour 50 mL of the solution into each pot.

[0061] The results are as follows Figure 8-11 As shown, alfalfa plants treated with Bacillus vesiculosus BV17 were susceptible to infection by the pathogen Phytophthora infestans (Phytophthora infestans). Phytophthora cactorum Under the infection conditions of ), its survival rate and fresh weight were significantly higher than those of the control plant ( Figure 8 ); in the pathogenic fungus Fusarium solanum ( Fusarium solani Under the infection conditions of ), its survival rate and fresh weight were significantly higher than those of the control plant ( Figure 9 ); in the pathogenic fungus Fusarium oxysporum ( Fusarium oxysporum Under infection conditions, although the survival rate of alfalfa after application of Bacillus belye BV17 was not significantly different from that of the control, its fresh weight was significantly higher than that of the control plant. Figure 10 ); in the pathogenic fungus Rhizoctonia solani ( Rhizoctonia solani Under the infection conditions of ), its survival rate and fresh weight were significantly higher than those of the control plant ( Figure 11 Therefore, it is shown that the application of Bacillus belye BV17 can effectively prevent and control the pathogenic oomycete Phytophthora (…). Phytophthora cactorum ), pathogenic fungus Fusarium solanum ( Fusarium solani Fusarium oxysporum ( Fusarium oxysporum ) and Rhizoctonia solani ( Rhizoctonia solani Alfalfa plant diseases caused by ).

[0062] Example 8

[0063] Field efficacy experiment of Bacillus belye BV17 was conducted in a 6m*6m plot with three treatments. Treatment one was OD1. 600 Treatment 1.0% bacterial suspension; Treatment 2. 800-fold diluted Bacillus belyss C17271 (Zhejiang Serike Cell Technology Co., Ltd., Registration Certificate No. PD20250010); Treatment 3. Water control. All treatments were applied simultaneously with seed sowing. At the initial flowering stage of alfalfa (“Huaiyang No. 4”), three 1m³ samples were randomly selected from each plot. 2 The quadrats were replicated three times, and the plant height, stem diameter, stem-to-leaf ratio, leaf area, and plot yield of the first crop of alfalfa were recorded. From planting to harvest, field sampling was conducted regularly to analyze root rot disease incidence.

[0064] The results are shown in Table 1. The alfalfa plant plots treated with Bacillus beryl BV17 showed increased yield and good control of root rot in the field.

[0065] Table 1. Field sampling analysis results

[0066] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A plant endophytic Bacillus belesia, characterized in that, The plant endophytic Bacillus belesii is BV17, classified and named Bacillus belesii. Bacillus velezensis The accession number is CGMCC No. 37617, and it was deposited at the China General Microbiological Culture Collection Center on February 2, 2026.

2. A microbial bactericide, characterized in that, The microbial bactericide contains the plant endophytic Bacillus belye as described in claim 1.

3. The application of the plant endophytic Bacillus belye as described in claim 1 or the microbial fungicide as described in claim 2, characterized in that, The application is any of the following: A1) Inhibits the growth of plant pathogens; A2) Prepare products that inhibit plant pathogens; A3) Prevention and control of plant diseases caused by plant pathogens; A4) Prepare products for preventing and treating plant diseases caused by plant pathogens.

4. The application according to claim 3, characterized in that, The plant pathogens include pathogenic fungi, pathogenic oomycetes, and pathogenic bacteria.

5. The application according to claim 4, characterized in that, The pathogenic fungus was selected from Fusarium graminearum (Fusarium graminearum). Fusarium graminearum Fusarium oxysporum ( Fusarium oxysporum Fusarium solani () Fusarium solani ), Fusarium graminearum ( Fusarium pseudograminearum Verticillium dahliae Verticillium dahliae Rhizoctonia solani ( ) Rhizoctonia solani ) and gray mold ( Botrytis cinerea One or more of the following.

6. The application according to claim 4, characterized in that, The pathogenic oomycete was selected from *Pythium tertrum* (… Pythium ultimum ), Phytophthora capsici ( Phytophthora capsici ), Phytophthora ( Phytophthora cactorum ), Phytophthora parasiticum ( Phytophthora parasitica ), soybean phytotoxicum ( Phytophthora sojae One or more of the following.

7. The application according to claim 4, characterized in that, The pathogenic bacteria were selected from *Pseudomonas syringae* tomato pathogenic strain (… Pseudomonas syringae pv. tomato Kiwi fruit canker pathogen ( Pseudomonas syringae pv. actinidae ) and Ralstonia solanacearum ( Ralstonia solanacearum One or more of the following.

8. A method for preventing and controlling plant diseases caused by plant pathogens, characterized in that, The method includes treating plant tissues with the plant endophytic Bacillus belye as described in claim 1 or the microbial bactericide as described in claim 2.

9. The method according to claim 8, characterized in that, The plant disease is caused by at least one of the pathogens described in claims 5-7.

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