Beauveria bassiana Bbsre1 gene knockout strain and application thereof

By constructing and transforming a BbSre1 gene knockout strain of Beauveria bassiana, the SREBP transcription factor BbSre1 was disrupted, promoting the production of antibacterial substances. This solved the problem of the difficulty in discovering the hidden secondary metabolites, achieving effective inhibition of plant pathogenic fungi and expanding the application of Beauveria bassiana.

CN122445684APending Publication Date: 2026-07-24CHONGQING UNIV OF TRADITIONAL CHINESE MEDICINE +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHONGQING UNIV OF TRADITIONAL CHINESE MEDICINE
Filing Date
2026-04-23
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

Existing technologies are insufficient to effectively discover latent secondary metabolites in Beauveria bassiana, which limits the development and application of new drugs. Furthermore, there is a lack of methods for screening latent secondary metabolites using gene knockout induction.

Method used

By constructing a recombinant vector expressing the homologous arm of the BbSre1 gene, transforming it into wild-type Beauveria bassiana, screening out the BbSre1 gene knockout strain ΔBbSre1, and obtaining a mutant strain using homologous recombination, the coding region of the SREBP transcription factor BbSre1 was disrupted, promoting the production of antibacterial substances.

Benefits of technology

The ΔBbSre1 strain showed significant inhibitory effects on plant pathogenic fungi such as gray mold, rapeseed black spot fungus, and Fusarium graminearum, expanding the application scope of Beauveria bassiana and providing a new approach for fungicide development.

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Abstract

The present application belongs to the field of genetic engineering, and relates to a Beauveria bassiana BbSre1 gene knockout strain and application thereof. The technical problem to be solved by the present application is to expand the application range of the Beauveria bassiana by studying the homologous proteins of SREBPs in the Beauveria bassiana. The technical scheme of the present application is a Beauveria bassiana BbSre1 gene knockout strain Beauveria bassiana Delta BbSre1, and the preservation number of the strain is CGMCC No. 42325. The anti-fungal substance produced by the Delta BbSre1 strain has a good inhibitory effect on plant pathogenic fungi.
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