Optimized rare ginsenoside catalytic synthesis method and application thereof

By optimizing the co-catalytic reaction system of glycosyltransferase and sucrose synthase, the problem of low production efficiency of rare ginsenosides was solved, and efficient catalytic synthesis was achieved to meet market demand for rare ginsenosides.

CN122445758APending Publication Date: 2026-07-24GSYNBIOT (SHANGHAI) CO LTD
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Patent Information

Application Number
CN202510118976.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-24
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

Existing technologies are insufficient for the efficient production of rare ginsenosides, and traditional methods suffer from limitations in yield and complex purification processes, making it difficult to meet market demand.

Method used

By optimizing the co-catalytic reaction system of glycosyltransferase and sucrose synthase, and utilizing enzymes from plants and microorganisms, combined with co-expression or fusion expression methods, the production efficiency of rare ginsenosides can be improved, achieving highly efficient catalytic synthesis.

Benefits of technology

This method increases the substrate conversion rate of rare ginsenosides from about 20% to more than 95%, providing a universal production method applicable to the industrial production of various rare ginsenosides.

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Abstract

The invention relates to an optimized rare ginsenoside catalytic synthesis method and application thereof. Specifically, the invention provides a reaction system for producing rare ginsenoside through co-catalysis of glycosyl transferase and sucrose synthase, enzyme is compared, mutated and screened, the activity and co-expression mode of glycosyl transferase and sucrose synthase are optimized, and the rare ginsenoside is obtained by further optimizing the concentration of crude enzyme, improving the solubility of a substrate and the like. The production efficiency of rare ginsenoside is improved, and the substrate conversion rate is increased from about 20% to more than 95%. The method disclosed by the invention is suitable for producing different types of rare ginsenosides, and has very high industrial production and application values.
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