An electrode device for collecting electrical activity of a brain region and a method of manufacturing the same

Abstract

The present application relates to the technical field of animal experiment electrode device, and more particularly to an electrode device for collecting brain region electrical activity and a preparation method thereof, the electrode device is matched by electrode wires and two abutting walls of a constraint groove, so that each electrode wire forms an arc-shaped buffer part, the arc-shaped buffer part can slide along the length direction of the electrode wire with the change of stress, thereby buffering and absorbing the pulling force of the electrode wire, and the pulling force will not be transmitted to the connection position of each electrode wire and the connector, so as to ensure the connection reliability between the electrode wire and the connector, meanwhile, the pins of the connector will not be damaged due to pulling, which is beneficial to long-term and stable collection of mouse brain signals. The present application also provides a preparation method for preparing the electrode device for collecting brain region electrical activity, the preparation method comprises the following steps: a mounting frame providing step, an electrode wire mounting and constraining step, a connector mounting step, an electrode wire welding step and an electrode wire fixing step.

Description

Technical Field

[0001] This invention relates to the field of animal experimental electrode devices, and more particularly to an electrode device for collecting electrical activity in brain regions. The invention also relates to a method for preparing this electrode device. Background Technology

[0002] In neuroscience research, local field potential (LFP) signals are important electrophysiological indicators reflecting the linear sum of postsynaptic potentials of neurons in a local brain region. They are widely used in areas such as neural circuit function analysis, brain disease mechanism research, and neuropharmacological efficacy evaluation. To obtain LFP signals from different deep brain regions (such as the CA1 region of the hippocampus and the DG region of the hippocampus), it is usually necessary to precisely implant recording electrodes into the target brain region and maintain stable electrical signal acquisition capabilities after surgery.

[0003] Currently, some electrode devices are equipped with adjustable implantation depth to accommodate recording needs in different brain regions or at different depths within the same brain region. However, existing electrode devices with adjustable depth typically use soldering to connect the electrode wire to the connector (e.g., a miniature connector for an external preamplifier). During adjustments to the electrode implantation depth or animal activity, the electrode wire is easily stretched, and the wiring path often lacks effective stress buffering structures. This causes the tensile force to be directly transmitted to the solder joint between the electrode wire and the connector pins, resulting in the solder joint being subjected to long-term stress and prone to breakage and detachment. This not only affects the stability of signal acquisition but may also damage the connector, increasing experimental costs.

[0004] Therefore, there is an urgent need to provide an electrode device for collecting electrical activity in brain regions, which can effectively avoid electrode wire traction caused by adjusting the depth or animal activity, prevent damage to the connection between the electrode wire and the connector, and ensure long-term stable signal acquisition while allowing the electrode implantation depth to be adjustable. Summary of the Invention

[0005] To address the aforementioned issues, this application discloses an electrode device for collecting electrical activity in brain regions. The device utilizes the cooperation of two abutting walls between the electrode wire and the constraint groove to form an arc-shaped buffer section for each electrode wire. This arc-shaped buffer section can slide along the length of the electrode wire as the force on the wire changes, thereby buffering and absorbing the tensile force on the electrode wire and preventing it from being transmitted to the connection points between the electrode wires and the connector. This ensures the reliability of the connection between the electrode wire and the connector. Simultaneously, it prevents damage to the connector pins due to tension, which is beneficial for long-term stable acquisition of signals from the mouse brain.

[0006] To achieve the above objectives, the electrode device for collecting brain region electrical activity adopts the following technical solution, comprising: a mounting frame, a wire bundle, a connector, and several electrode wires; the mounting frame includes a constraint part and a mounting part, the constraint part having a constraint groove with a pair of opposing abutment walls, and the mounting part having a sliding channel and a wire passage groove; the wire bundle is slidably disposed within the sliding channel, and the wire bundle has several mounting channels; the connector is detachably located on one side of the mounting part; several electrode wires are respectively partially fixed to corresponding mounting channels, wherein the several electrode wires One end of each electrode wire extends from the corresponding mounting channel for implantation into the brain region. Several electrode wires are also partially bent within the constraint groove and abut against a pair of abutment walls to form an arc-shaped buffer portion, allowing the other end of the electrode wires to pass through the wire passage and be electrically connected to the connector. As the wire bundle moves, each electrode wire is pulled by the wire bundle, and under the constraint of the pair of abutment walls, the formation position of each arc-shaped buffer portion slides along the extension direction of the corresponding electrode wire to buffer the movement of the electrode wire relative to the constraint portion.

[0007] In one illustrative embodiment of the electrode device for collecting electrical activity in brain regions, the mounting portion further includes a mounting chamber, through which the mounting channel passes; The electrode device for collecting electrical activity in brain regions also includes: An adjusting member, rotatably connected to the mounting portion and extending into the mounting cavity; and A connector threadedly connected to the adjusting member, wherein the connector is fixedly connected to the cable bundle, and when the adjusting member rotates relative to the mounting portion, the connector moves axially along its extension direction under the drive of the adjusting member.

[0008] In one illustrative embodiment of the electrode device for collecting electrical activity in brain regions, the circumferential outer surface of the connector is composed of several planes, wherein at least one of the planes is parallel to and abuts against the inner surface of the mounting chamber.

[0009] In one illustrative embodiment of the electrode device for collecting electrical activity in brain regions, the electrode device further includes a cover plate, wherein the cover plate is connected to the mounting portion and covers the constraint groove.

[0010] In one illustrative embodiment of the electrode device for collecting electrical activity in brain regions, the cover plate includes a covering part and a connecting part. The covering part is used to cover the constraint groove, the connecting part has a through hole, and the mounting part also has a threaded hole corresponding to the through hole. The electrode device for collecting brain region electrical activity also includes a fixing bolt, which passes through the through hole and is screwed into the threaded hole to fix the connecting part to the mounting part.

[0011] In one illustrative embodiment of the electrode device for collecting electrical activity in brain regions, the connecting portion further includes an operating channel, wherein, along the extending direction of the operating channel, the operating channel includes an operating section and a limiting section, wherein the diameter of the operating section is smaller than that of the limiting section; The adjusting member is provided with an operating end, and a force-applying groove is provided on the top end face of the operating end. After the connecting part is connected to the mounting part, the operating end is located within the limiting section, and the force-applying groove is exposed within the operating section.

[0012] In one illustrative embodiment of an electrode device for collecting electrical activity in brain regions, the wire bundle includes: a main body and several electrode mounting tubes of different lengths, the main body having a first end and a second end opposite to each other, wherein, along the extension direction of the mounting channel, the first end is located above the second end. Several electrode mounting tubes are disposed inside the main tube, with one end of each electrode mounting tube flush with the first end, and the other end of each electrode mounting tube extending out of the main tube from the second end. The mounting channel is formed inside the electrode mounting tube. Each of the electrode wires extends from the port of the electrode mounting tube away from the first end, and the distance between the end of each electrode wire and the first end is different.

[0013] In one illustrative embodiment of the electrode device for collecting electrical activity in brain regions, the electrode device further includes a bottom-sealed housing, wherein the bottom-sealed housing seals the bottom of the connector along the height direction of the mounting portion.

[0014] The present invention also provides a method for preparing an electrode device for collecting electrical activity in brain regions, the method comprising the following steps: Providing the mounting bracket: Providing one of the aforementioned mounting brackets; Electrode wire installation and constraint steps: Place the wire bundle tube into the sliding channel, and sequentially pass one end of each electrode wire through each installation channel of the wire bundle tube. Then, fold the other end of the electrode wire back in the horizontal direction and pass it through the wire groove, so that each electrode wire bends in the constraint groove to form an arc-shaped buffer part that abuts against the walls on both sides of the constraint groove. Connector installation steps: Apply adhesive to one side of the connector and / or one side of the mounting part, attach the connector to one side of the mounting part, and after the adhesive has solidified, the connector and the mounting part are connected. Electrode wire welding steps: Pass one end of each electrode wire through the wire groove and weld each pin of the connector in turn. Electrode wire fixing steps: Apply adhesive to the position where each electrode wire passes through the corresponding mounting hole, or inject adhesive into each mounting hole. After the adhesive solidifies, each electrode wire will be fixedly connected to the corresponding mounting hole.

[0015] In one illustrative embodiment of the method for preparing an electrode device for collecting electrical activity in brain regions, the mounting portion further includes a mounting chamber, and the mounting channel passes through the mounting chamber; The electrode wire installation constraint step further includes: before the wire bundle tube is installed into the sliding channel, a connector is placed in the installation cavity, and an adjusting member is rotatably installed in the installation part, one end of the adjusting member extending into the installation cavity along the extension direction of the sliding channel and threadedly connected to the connector; After the cable bundle is installed into the sliding channel, an adhesive is applied to the outer surface of the connector and / or the outer surface of the cable bundle. After the adhesive cures, the connector is bonded to the cable bundle.

[0016] Beneficial effects: 1. Each electrode wire of the electrode device used to collect brain region electrical activity forms an arc-shaped buffer part by abutting against two abutting walls in the constraint groove. When the wire bundle moves or the animal moves and generates traction force, the arc-shaped buffer part can slide along the length of the electrode wire to absorb and buffer the displacement and tension. This can prevent direct conduction to the connection position between the electrode wire and the connector, thereby preventing the connection position from breaking or falling off. This is beneficial for long-term stable acquisition of mouse brain signals. At the same time, it can also protect the connector from damage and is beneficial for the secondary use of the connector.

[0017] By cooperating with the adjusting components, connectors, and wire bundle tubes, the wire bundle tubes can be driven to move axially along the sliding channel by rotating the adjusting components. This allows for precise fine-tuning of the implantation depth of each electrode wire simultaneously, improving experimental efficiency and meeting different experimental needs.

[0018] By setting up a cable bundle with multiple mounting channels, adjacent electrode wires can be physically isolated, making it easier for experimenters to identify the wiring and ensuring that the signals of each channel of the connector correspond precisely to the corresponding electrode wire, thereby improving the accuracy of data management.

[0019] By setting a cover plate to cover the constraint groove, the contact between each electrode wire and the outside world can be reduced, thus reducing the risk of contamination and infection. At the same time, the electrode wires are protected from damage by mice, and some external electromagnetic noise can be isolated, reducing signal interference.

[0020] 2. In the above preparation method, after each electrode wire is inserted into the corresponding mounting channel, it forms an arc-shaped buffer part by its own elastic bending and abuts against the wall of the constraint groove. This can temporarily stabilize the position of the wire bundle tube before it is fixed, without the need to use other tools to impose additional restrictions on the wire bundle tube, which is convenient for subsequent welding and connector installation. Furthermore, since each electrode wire is not fixed in advance in the mounting channel, the operator can flexibly adjust the extension amount of each electrode wire through the wire groove to adapt to different welding requirements.

[0021] By constraining the electrode wire installation, each electrode wire is bent to form an arc-shaped buffer section, which can absorb the displacement of the wire harness brought by the wire harness. This prevents the tensile force caused by the movement of the wire harness from being directly transmitted to the welding position between each electrode wire and the connector, thereby reducing the wear on the connector during the manufacturing process and extending its service life.

[0022] The following description, in a clear and easy-to-understand manner and with reference to the accompanying drawings, will further explain the above-mentioned characteristics, technical features, advantages, and implementation methods of an electrode device for collecting electrical activity in brain regions. Attached Figure Description

[0023] Various other advantages and benefits will become apparent to those skilled in the art upon reading the following detailed description of preferred embodiments. The accompanying drawings are for illustrative purposes only and are not intended to limit the scope of this application. Furthermore, the same reference numerals denote the same parts throughout the drawings. In the drawings: Figure 1 This is a schematic structural diagram illustrating one embodiment of an electrode device for collecting electrical activity in brain regions.

[0024] Figure 2 This is a schematic diagram illustrating the structure of the arc-shaped buffer section.

[0025] Figure 3 This diagram illustrates the structural relationship between the cable tie tube and the mounting section.

[0026] Figure 4 A schematic diagram illustrating one embodiment of the adjusting member and the connecting member.

[0027] Figure 5 Used to explain Figure 4 Cross-sectional view along the AA direction.

[0028] Figure 6A structural schematic diagram illustrating one possible embodiment of the cover plate.

[0029] Figure 7 A schematic diagram illustrating one possible embodiment of the wire harness tube.

[0030] Figure 8 A schematic diagram illustrating one embodiment of the bottom-closed housing.

[0031] Figure 9 A flowchart illustrating an illustrative embodiment of a method for preparing an electrode device for collecting electrical activity in brain regions.

[0032] Figure 10 This diagram illustrates the bending process of the electrode wire during the electrode wire installation and constraint steps.

[0033] Label Explanation 1. Mounting bracket; 11. Constraint part; 111. Constraint groove; 112. Abutment wall; 12. Mounting part; 121. Sliding channel; 122. Wire passage groove; 123. Mounting chamber; 124. Threaded hole; 2. Adjusting component; 21. Connecting component; 3. Cable bundle tube; 31. Main body; 32. Electrode mounting tube body; 321. Mounting channel; 4. Electrode wire; 41. Arc-shaped buffer part; 5. Connector; 6. Cover plate; 61. Connecting part; 62. Covering part; 7. Bottom closed shell; 71. Clearance groove; S1. Mounting bracket provision step; S2. Electrode wire installation and constraint step; S3. Connector installation step; S4. Electrode wire welding step; S5. Electrode wire fixing step. Detailed Implementation

[0034] To provide a clearer understanding of the technical features, objectives, and effects of this application, specific embodiments of this application are now described with reference to the accompanying drawings. In the drawings, the same reference numerals indicate components with the same or similar structures but the same function.

[0035] In this document, “illustrative” means “serving as an example, illustration or description”, and any illustration or implementation described herein as “illustrative” should not be construed as a more preferred or advantageous technical solution.

[0036] To keep the drawings concise, only the parts relevant to this application are shown schematically in each drawing, and they do not represent the actual structure of the product. In addition, to make the drawings concise and easy to understand, in some drawings, only one of the components with the same structure or function is shown schematically, or only one of them is labeled.

[0037] Figure 1 This is a schematic structural diagram illustrating one embodiment of an electrode device for collecting electrical activity in brain regions. Figure 2This is a schematic diagram illustrating the structure of the arc-shaped buffer section. Figure 3 This diagram illustrates the structural relationship between the cable tie and the mounting section. (Combined with...) Figures 1-3 The electrode device used to collect electrical activity in brain regions includes: a mounting frame 1, a cable bundle 3, a connector 5, and seven electrode wires 4; Figure 1 As shown, the mounting bracket 1 includes a constraint part 11 and a mounting part 12. The constraint part 11 has a constraint groove 111 along... Figures 1-2 In the X direction, the left and right sides of the constraint groove 111 are opposite to the abutment wall 112.

[0038] The mounting section 12 is provided with a sliding channel 121 and a wire guide groove 122, along which... Figure 1-2 In the X direction, the sliding channel 121 and the wire groove 122 are spaced apart, along... Figures 1-2 In the Y direction, the sliding channel 121 and the wire passage 122 extend from the top surface of the mounting part 12 to the bottom surface. The position of the sliding channel 121 and the wire passage 122 on the mounting part 12 corresponds to the bottom of the constraint groove 111, and the sliding channel 121 and the wire passage 122 are respectively close to the two abutment walls 112.

[0039] Cable bundle 3 along Figures 1-2 The cable bundle 3 is slidably inserted in the sliding channel 121 in the Y direction. The cable bundle 3 has 7 mounting channels 321, each of which is independent of the others.

[0040] As shown in the figure, along Figures 1-2 In the X direction, connector 5 can be installed to the left side of mounting part 12 by adhesive bonding. Connector 5 can be NSD-18-DD-GS (18-pin dual-row straight-through nano-micro connector), such as... Figure 1 As shown, the connector 5 has two rows of sockets (connection channels) on the top for connecting an external preamplifier, and 18 pins on the bottom for connecting electrode wire 4.

[0041] Combination Figures 1-3 Each electrode wire is fixed in four parts into the corresponding mounting holes 321, such as... Figure 1-2 As shown, one end of each electrode wire 4 protrudes through the corresponding mounting hole 321 for implantation into the brain region of a mouse during use. Referring to Figure 2, the other end of each electrode wire 4 extends along... Figure 2 The Y-direction wires are folded downwards and pass through the wire groove 122 to connect to the corresponding pins on the connector 5. Specifically, each electrode wire 4 is soldered to the corresponding pins on the connector 5.

[0042] Under the above settings, such as Figures 1-2As shown, each electrode wire 4 is partially bent within the constraint groove 111, and the bent portion abuts against two abutment walls 112 respectively. At this time, under the restriction of the two abutment walls 112, each electrode wire 4 naturally bends into an arc shape within the constraint groove 111, thereby forming an arc-shaped buffer portion 41 on each electrode wire 4.

[0043] like Figures 1-2 As shown, since each electrode wire 4 is partially fixed to the corresponding mounting channel 321, when the wire harness 3 moves, for example along... Figure 2 When the cable bundle 3 moves downward in the Y direction, it will cause each electrode wire 4 to move. During this process, each arc-shaped buffer part 41 will slide along the length of the electrode wire 4 under the constraint of a pair of abutment walls 112. That is, the arc-shaped buffer part 41 will be closer to the end connected to the connector 5 on the electrode wire 4, so that the pulling force caused by the movement of the cable bundle 3 will not be directly transmitted to the connection position between each electrode wire 4 and the connector 5.

[0044] As can be seen, under the above configuration, the constraint groove 111 cooperates with the two abutment walls 112 to form an arc-shaped buffer part 41 for each electrode wire 4. This arc-shaped buffer part 41 can slide along the length of the wire as the force on the electrode wire 4 changes, thereby absorbing the displacement of the wire 4 brought by the wire harness tube 3 by the positional change of the arc-shaped buffer part 41. This prevents the pulling force caused by the movement of the wire harness tube 3 from being directly transmitted to the connection position between each electrode wire 4 and the connector 5. In use, it can effectively buffer the movement of the wire harness tube 3 or the displacement caused by animal activity, avoid tugging at the connection position between each electrode wire 4 and the connector 5, and prevent each electrode wire from being pulled. Unexpected bending occurs at the connection point of connector 5, protecting the reliability of the connection between electrode wire 4 and the pins of connector 5, and ensuring that the pins of connector 5 are not damaged by electrode wire 4, thus facilitating the secondary use of connector 5. In addition, the above-mentioned arrangement integrates multiple functions such as electrode wire 4 constraint, electrode wire 4 implantation guidance, and connector 5 fixation into the mounting frame 1, reducing the use of additional parts, making the overall device small in size and light in weight, and allowing it to be firmly bonded to the mouse skull, reducing the impact on the mouse's daily activities, and facilitating long-term EEG activity acquisition of mice in a free-moving state.

[0045] In actual production, each electrode wire 4 is made of double-strand tungsten wire. Each electrode wire 4 has two joint ends that are welded to the connector 5. The joint ends of each electrode wire 4 are welded to the corresponding pins on the connector 5.

[0046] Figure 4 A schematic diagram illustrating one embodiment of the adjusting member and the connecting member. Figure 5 Used to explain Figure 4A cross-sectional view along the AA direction. To enable the cable bundle 3 to move within the sliding channel 121, the electrode device for collecting brain region electrical activity also includes: an adjusting element 2 and a connecting element 21; as shown... Figure 4 As shown, the mounting part 12 also has a mounting chamber 123 inside. The mounting chamber 123 has an opening on the outer surface of the mounting part 12, and the mounting channel 321 passes through the mounting chamber 123. The adjusting member 2 is rotatably connected to the mounting part 12, and one end of the adjusting member 2 extends into the mounting chamber 123. The connecting member 21 is located in the mounting chamber 123 and is threadedly connected to the adjusting member 2. (Refer to...) Figure 5 The outer surface of the connector 21 is fixedly connected to the outer surface of the cable tie tube 3. When the adjusting member 2 rotates relative to the mounting part 12, since the connector 21 is fixedly connected to the cable tie tube 3, the cable tie tube 3 can restrict the connector 21, so that the connector 21 does not rotate synchronously with the adjusting member 2, but moves axially along the extension direction of the adjusting member 2 under the threaded engagement with the adjusting member 2. Figure 1 (Y direction in the image). This configuration allows the operator to flexibly control the depth of each electrode wire 4 implanted into the mouse brain, thereby enabling the localization and recording of specific neural regions. Furthermore, the threads themselves possess self-locking properties, ensuring that the cable bundle 3 remains stably in its current position after adjustment, preventing spontaneous slippage due to mouse movement or gravity, thus ensuring long-term stability of the implantation depth and obtaining more stable and reliable neural signal recordings. Specifically, the connector 21 and the cable bundle 3 are connected by adhesive bonding; of course, as those skilled in the art will understand, the above configuration requires the cable bundle 3 to be a rigid tube.

[0047] To further improve the stability of the connection between connector 21 and cable bundle 3, combined with Figure 5 The connector 21 is a hexagonal nut. In this configuration, the outer circumferential surface of the connector 21 is composed of six continuous planes. At least one plane of the connector 21 is parallel to the inner surface of the mounting chamber 123 within the mounting groove, and this plane also abuts against the inner surface of the mounting chamber 123. In this configuration, when the adjusting member 2 is rotated, the torque generated by the rotation of the adjusting member 2 is applied to the inner surface of the mounting chamber 123, rather than concentrated at the connection point between the connector 21 and the cable tie 3. This prevents breakage due to stress concentration at the connection point, thus ensuring the connection strength between the connector 21 and the cable tie 3. It will be understood by those skilled in the art that, in the above configuration, the cable tie 3 can also be a flexible tube.

[0048] Figure 6 A structural schematic diagram illustrating one possible embodiment of the cover plate. For example... Figure 6 As shown, the electrode device for collecting electrical activity in brain regions further includes: a cover plate 6, wherein the cover plate 6 is detachably connected to the mounting portion 12 and can cover the constraint groove 111; combined with Figure 1 and Figure 6 Specifically, the cover plate 6 includes a covering part 62 and a connecting part 61. The covering part 62 is used to cover the constraint groove 111 to isolate the electrode wire 4 inside the constraint groove 111 from the outside. In this arrangement, part of the electrode wire 4 is located in the mounting hole 321 of the wire bundle tube 3 and part is located in the closed constraint groove 111, thereby minimizing the contact area between the electrode wire 4 and the outside, thus reducing the probability of contamination of the electrode wire 4 and the infection rate of mice. At the same time, it can also protect the electrode wire 4 from damage by mice during the experiment. In addition, the covering part 62 can also isolate some electromagnetic noise from the outside, thereby reducing the interference of the outside world on the electroencephalogram signals of mice acquired by the electrode wire 4.

[0049] like Figure 6 As shown, the mounting part 12 is also provided with a threaded hole 124, and the connecting part 61 of the cover plate 6 is also provided with a through hole corresponding to the threaded hole 124. In use, a fixing bolt is screwed into the threaded hole 124 after passing through the through hole until the head of the fixing bolt abuts against the outer surface of the connecting part 61, so that the entire cover plate 6 and the mounting part 12 form a stable connection relationship.

[0050] like Figure 6 As shown, the connecting part 61 also has an operating channel, which, along its extending direction, includes an operating section and a limiting section. The diameter of the operating section is smaller than that of the limiting section, such as... Figure 1 and Figure 6 As shown, the adjusting member 2 also has an operating end, the diameter of which is the same as the diameter of the limiting section. After the connecting part 61 is connected to the mounting part 12, the operating end is located within the limiting section, thus preventing the adjusting member 2 from moving undesirably relative to the mounting part 12. Correspondingly, this also makes the position of the wire bundle 3 within the sliding channel 121 more stable. The operating section connects the limiting section to the outside. After the operating section of the adjusting member 2 is restricted, the force groove of the operating end of the adjusting member 2 can be seen through the operating section of the operating channel. In actual use, a screwdriver is inserted into the force groove through the operating section to twist the adjusting member 2, thereby adjusting the implantation depth of each electrode wire 4. With the above configuration, not only can the constraint groove 111 be sealed, but relative movement of the adjusting member 2 relative to the mounting part 12 can also be avoided, thus ensuring the long-term stability of the electrode wire 4 in the mouse brain, which is conducive to obtaining more stable and reliable neural signal recording.

[0051] Figure 7This is a structural schematic diagram illustrating one possible embodiment of a wire harness. (Combined with...) Figure 7 The cable bundle 3 includes: a main body 31 and seven electrode mounting tubes 32 of different lengths. The main body 31 has a first end and a second end opposite to each other, extending along the extension direction of the mounting channel 321. Figure 1-2 (in the Y direction), the first end is located above the second end.

[0052] Each electrode mounting tube 32 is fixedly mounted on the main tube 31. One end of each electrode mounting tube 32 is flush with the main tube 31, and the other end extends out of the main tube 31. Mounting channels 321 are formed within the electrode mounting tube 32. Figure 7 As shown, each electrode wire 4 extends from the port of the electrode mounting tube 32 away from the first end, and the extension length of each electrode wire 4 is different. In this configuration, since the extension length of each electrode wire 4 from the corresponding electrode mounting tube 32 is different, multiple electrode wires 4 can be implanted into brain target points at different depths at the same time, thereby realizing the simultaneous acquisition of local field potential signals of different deep brain regions.

[0053] In addition, each mounting channel 321 is independent of each other, thus forming a physical isolation between adjacent electrode wires 4. This makes it easy for experimental personnel to identify the direction of each electrode wire 4, and helps the EEG signals collected by each channel of the connector 5 to accurately correspond to the corresponding electrode wire 4, thereby improving the accuracy of data management.

[0054] Specifically, the electrode wire 4 can be partially fixed in the mounting hole 321 of the corresponding electrode mounting tube 32 by means of adhesive bonding or other methods.

[0055] Figure 8 This is a schematic diagram illustrating one embodiment of the bottom-sealed shell. During installation, the electrode device for collecting brain region electrical activity typically involves pre-applying dental cement to the exposed skull of a mouse, then attaching the bottom of the mounting bracket 1 (or the bottom of the mounting part 12) to the mouse's head using the dental cement. After installation, the bottom of the connector 5 remains close to the mouse's skull. During prolonged experiments, bodily fluids secreted by the mouse's head may come into contact with the connection points between the electrode wires 4 and the connector, thus affecting the transmission of the mouse's electroencephalogram (EEG) signals. Therefore, in conjunction with... Figure 8 The electrode device for collecting brain region electrical activity also includes: a bottom-sealed housing 7, which is used to seal the connection points between each electrode wire 4 and the connector 5, specifically, as shown in the figure. Figure 8 As shown, the bottom closed housing 7 has a receiving groove for accommodating part of the electrode wire 4 and the pins of the connector 5, so that they do not come into contact with the outside. The bottom closed housing 7 is connected to the mounting part 12 by means of adhesive.

[0056] Of course, in other embodiments, the connection points of each electrode wire 4 and connector 5 are also protected in other ways. For example, dental cement is applied to the connection points of each electrode wire 4 and connector 5 to cover the connection points of each electrode wire 4 and connector 5 so that the pins of the electrode wire 4 and connector 5 do not come into direct contact with the mouse's head.

[0057] Figure 9 A flowchart illustrating an illustrative embodiment of a method for preparing an electrode device for collecting electrical activity in brain regions. Figure 10 This diagram illustrates the electrode wire bending process during the electrode wire installation and constraint steps. (Combined with...) Figures 9-10 The present invention also provides a method for preparing an electrode device for collecting electrical activity in brain regions, which is used to prepare the aforementioned electrode device for collecting electrical activity in brain regions, such as... Figure 9 As shown, the preparation method includes: step S1, providing the mounting bracket; step S2, mounting and constraining the electrode wire; step S3, installing the connector; step S4, welding the electrode wire; and step S5, fixing the electrode wire. Details are as follows: Step S1: Providing the mounting bracket step, providing the aforementioned mounting bracket 1, which can be made of resin material by 3D printing. The height direction of the mounting bracket 1 is... Figure 1 The Y direction in the diagram. Of course, the mounting bracket 1 can also be manufactured by other methods, such as turning, injection molding, etc.

[0058] Step S2: Electrode wire installation and constraint steps, combined with Figure 9 and Figure 10 In the direction perpendicular to the height of the mounting bracket 1, the mounting bracket 1 is placed horizontally, and the wire harness 3 is installed into the sliding channel 121 in the horizontal direction. One end of each electrode wire 4 is passed through the respective mounting channel 321 of the wire harness 3 in sequence, and the other end of the electrode wire 4 is folded back in the horizontal direction and passed through the wire groove 122, so that each electrode wire 4 is bent in the constraint groove 111 to form an arc-shaped buffer part 41 that abuts against the walls 112 on both sides of the constraint groove 111. Then proceed to step S3.

[0059] Specifically, such as Figure 7 as well as Figure 10 As shown, the cable bundle 3 consists of a main body 31 and seven electrode mounting tubes 32 of different lengths. Both the main body 31 and each electrode mounting tube 32 are PI tubes. The main body 31 is 8mm long, the longest electrode mounting tube 32 is 10mm long, and the lengths of adjacent electrode mounting tubes 32 differ by 0.1-0.2mm. When manufacturing the cable bundle 3, adhesive can be applied to the outer surface of each electrode mounting tube 32, and then each electrode mounting tube 32 is sequentially assembled into the main body 31. Figure 7In this configuration, one end of each electrode mounting tube 32 is flush with the main tube 31, and the other end extends out of the main tube 31 from the second end. The mounting channel 321 is the pipeline channel of the electrode mounting tube 32. After the glue solidifies, each electrode mounting tube 32 is fixed inside the main tube 31 to obtain the wire harness tube 3.

[0060] Step S3: Connector installation step. Apply adhesive to one side of connector 5 and attach connector 5 to one side of mounting part 12. After the adhesive hardens, connector 5 and mounting part 12 are connected. Connector 5 is NSD-18-DD-GS (18-pin dual-row straight-through nano-micro connector 5), such as... Figure 3 As shown, connector 5 has 18 pins and two rows of sockets (connection channels) for connecting external preamplifiers. In this step, combined with... Figure 1 and Figure 3 Since the wire groove 122 has an opening corresponding to the installation position of the connector 5, after the connector 5 is connected to the mounting part 12, the connector 5 can close the opening so that each electrode wire 4 is constrained in the relatively closed wire groove 122, thereby preventing each electrode wire 4 from coming out of the wire groove 122.

[0061] It should be noted that in step S3, the adhesive can also be applied to the outer surface of the mounting portion 12 of the mounting bracket 1, or the adhesive can be applied to both the outer surface of the connector 5 and the outer surface of the mounting portion 12 of the mounting bracket 1.

[0062] Step S4: Electrode wire welding step. Pass one end of each electrode wire 4 through the wire groove 122 and weld each pin of the connector 5 in turn. Specifically, flux can be applied to each pin of the connector 5 in advance, and then a small amount of solder can be drawn up with a soldering gun and the ends of each electrode wire 4 can be welded to the corresponding pins of the connector 5 in turn.

[0063] Step S5: Electrode wire fixing step, apply adhesive to the position where each electrode wire 4 passes through the corresponding mounting channel 321, or inject adhesive into each mounting channel 321. After the adhesive solidifies, each electrode wire 4 is fixedly connected to the corresponding mounting channel 321.

[0064] In the above preparation method, after one end of each electrode wire 4 is passed through the mounting channel 321, the mounting channel 321 can restrict the corresponding electrode wire 4, so that the operator can use the elasticity of the electrode wire 4 to bend each electrode wire 4 into an arc-shaped buffer part 41, and make the arc-shaped buffer part 41 abut against the two abutting walls 112 of the constraint groove 111. In this form, each electrode wire 4 is in close contact (abutting relationship) with the inner wall of the corresponding mounting channel 321 under its own elasticity. At the same time, the two abutting walls 112 of the constraint groove 111 not only restrict the arc-shaped buffer part 41 of each electrode wire 4, but also provide support for each electrode wire 4, thereby temporarily stabilizing the position of the wire bundle tube 3 in the sliding channel 121 during the preparation process, so that the operator can carry out subsequent preparation steps.

[0065] Secondly, each electrode wire 4 is partially located in the corresponding mounting channel 321. The arc-shaped buffer portion 41 of each electrode wire 4 is restricted by the two abutment walls 112 of the constraint groove 111, which can achieve double constraint on each electrode wire 4. Since the portion of the electrode wire 4 located in the corresponding mounting channel 321 is not fixed, the operator can stably pull each electrode wire 4 at the end that passes through the wire groove 122 to adjust the amount of each electrode wire 4 extending out of the wire groove 122, thereby facilitating the operator to carry out the subsequent electrode wire 4 welding steps.

[0066] Furthermore, the wire guide groove 122 has an opening corresponding to the installation position of the connector 5, which allows the operator to easily place the electrode wire 4 into the wire guide groove 122 through the opening. After the electrode wire 4 is placed into the wire guide groove 122, the connector 5 is connected to the mounting part 12. At this time, the connector 5 can close the opening, so that each electrode wire 4 is constrained in the relatively closed wire guide groove 122, thereby preventing each electrode wire 4 from coming out of the wire guide groove 122.

[0067] Furthermore, by utilizing the curing of the adhesive, a stable connection can be formed between each electrode wire 4 and its corresponding mounting channel 321. This allows the electrode wires 4 to move as the cable bundle 3 moves within the sliding channel 121, thereby enabling adjustment of the implantation depth of each electrode wire 4. Simultaneously, while adjusting the implantation depth of each electrode wire 4, the arc-shaped buffer portion 41, formed by bending using the aforementioned method, can slide along the wire length direction as the force on the electrode wire 4 changes. This buffers and absorbs the displacement of the electrode wires 4 caused by the cable bundle 3, preventing the tensile force from the movement of the cable bundle 3 from being directly transmitted to the welding positions of each electrode wire 4 and the connector 5. This reduces wear and tear on the device and extends its service life.

[0068] Combining the above and Figure 4 The mounting section 12 also includes a mounting chamber 123, through which the mounting channel 321 passes.

[0069] Combination Figures 3-5 and Figure 9 Step S2, the electrode wire installation and constraint step, further includes placing a connector 21 in the installation cavity before the cable bundle 3 is installed into the sliding channel 121, and rotatably installing an adjustment member 2 into the installation part 12. One end of the adjustment member 2 extends into the installation cavity 123 along the extension direction of the sliding channel 121 and is threadedly connected to the connector 21. After the cable bundle 3 is installed into the sliding channel 121, an adhesive is applied to the outer surface of the connector 21 and the outer surface of the main body 31 of the cable bundle 3. After the adhesive cures, the connector 21 is bonded to the main body 31 of the cable bundle 3.

[0070] Specifically, the adjusting member 2 can be a bolt, and the connecting member 21 can be a nut.

[0071] Specifically, the adhesives mentioned above are glues, such as AB glue, instant glue (502 glue), etc.

[0072] In the above steps, a spiral transmission mechanism is constructed by installing a rotatable adjusting component 2 and a threaded connecting component 21, and fixing the connecting component 21 to the wire harness tube 3. Rotating the adjusting component 2 can drive the wire harness tube 3 to move axially along the sliding channel 121, thereby enabling concentrated and precise fine-tuning of the implantation depth of multiple electrode wires 4. The operation is simple and has high resolution.

[0073] In addition, by using adhesive to bond the connector 21 to the wire harness tube 3 as a whole, and the threaded engagement between the adjusting member 2 and the connector 21, the position of the wire harness tube 3 in the sliding channel 121 can be constrained, thereby ensuring that the wire harness tube 3 will not move axially during the installation of the electrode wire 4, which facilitates the subsequent installation of the electrode wire 4.

[0074] It should be understood that although this specification describes various embodiments, not every embodiment contains only one independent technical solution. This way of describing the specification is only for clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

[0075] The detailed descriptions listed above are merely specific descriptions of feasible embodiments of this application and are not intended to limit the scope of protection of this application. All equivalent implementations or modifications made without departing from the spirit of the art of this application, such as combinations, divisions or repetitions of features, should be included within the scope of protection of this application.

Claims

1. An electrode device for collecting electrical activity in brain regions, characterized in that, It includes, The mounting bracket includes a constraint part and a mounting part. The constraint part has a constraint groove with a pair of opposing abutment walls. The mounting part has a sliding channel and a wire passage. A cable bundle tube is slidably disposed within the sliding channel, and the cable bundle tube is provided with several mounting channels; A connector that connects to one side of the mounting portion; as well as Several electrode wires are respectively fixed to the corresponding mounting channels. One end of each electrode wire extends out of the corresponding mounting channel for implantation into the brain region. The electrode wires are also partially bent within the constraint groove and abut against a pair of abutment walls to form an arc-shaped buffer portion, allowing the other end of each electrode wire to pass through the wire groove and be electrically connected to the connector. As the wire bundle moves, each electrode wire is pulled by the wire bundle, and under the constraint of a pair of abutment walls, the formation position of each arc-shaped buffer portion slides along the extension direction of the corresponding electrode wire to buffer the movement of the electrode wire relative to the constraint portion. The mounting part further includes a mounting chamber, and the mounting channel passes through the mounting chamber; The electrode device for collecting electrical activity in brain regions also includes: An adjusting member, rotatably connected to the mounting portion and extending into the mounting cavity; and A connector threadedly connected to the adjusting member, wherein the connector is fixedly connected to the cable bundle, and when the adjusting member rotates relative to the mounting portion, the connector moves axially along its extension direction under the drive of the adjusting member.

2. The electrode device for collecting electrical activity in brain regions as described in claim 1, characterized in that, The circumferential outer surface of the connector is composed of several planes, wherein at least one of the planes is parallel to the inner surface of the mounting chamber and abuts against the inner surface of the mounting chamber.

3. The electrode device for collecting electrical activity in brain regions as described in claim 1, characterized in that, The electrode device for collecting electrical activity in brain regions further includes a cover plate, wherein the cover plate is connected to the mounting portion and covers the constraint groove.

4. The electrode device for collecting electrical activity in brain regions as described in claim 3, characterized in that, The cover plate includes a covering part and a connecting part. The covering part is used to cover the constraint groove. The connecting part has a through hole. The mounting part also has a threaded hole corresponding to the through hole. The electrode device for collecting brain region electrical activity also includes a fixing bolt, which passes through the through hole and is screwed into the threaded hole to fix the connecting part to the mounting part.

5. The electrode device for collecting electrical activity in brain regions as described in claim 4, characterized in that, The connecting part is also provided with an operating channel, wherein, along the extending direction of the operating channel, the operating channel includes: an operating section and a limiting section, wherein the diameter of the operating section is smaller than that of the limiting section; The adjusting member is provided with an operating end, and a force-applying groove is provided on the top end face of the operating end. After the connecting part is connected to the mounting part, the operating end is located within the limiting section, and the force-applying groove is exposed within the operating section.

6. The electrode device for collecting electrical activity in brain regions as described in claim 1, characterized in that, The wire harness includes: a main body and several electrode mounting tubes of different lengths, the main body having a first end and a second end opposite to each other, wherein, along the extension direction of the mounting channel, the first end is located above the second end; Several electrode mounting tubes are disposed inside the main tube, with one end of each electrode mounting tube flush with the first end, and the other end of each electrode mounting tube extending out of the main tube from the second end. The mounting channel is formed inside the electrode mounting tube. Each of the electrode wires extends from the port of the electrode mounting tube away from the first end, and the distance between the end of each electrode wire and the first end is different.

7. The electrode device for collecting electrical activity in brain regions as described in claim 1, characterized in that, The electrode device for collecting electrical activity in brain regions further includes a bottom-sealed housing, wherein the bottom-sealed housing seals the bottom of the connector along the height direction of the mounting portion.

8. A method for preparing an electrode device for collecting electrical activity in brain regions, characterized in that, For preparing the electrode device for acquiring electrical activity in brain regions as described in claim 1, it comprises: Providing the mounting bracket: Providing one of the aforementioned mounting brackets; Electrode wire installation and constraint steps: Place the wire bundle tube into the sliding channel, and sequentially pass one end of each electrode wire through each installation channel of the wire bundle tube. Then, fold the other end of the electrode wire back in the horizontal direction and pass it through the wire groove, so that each electrode wire bends in the constraint groove to form an arc-shaped buffer part that abuts against the walls on both sides of the constraint groove. Connector installation steps: Apply adhesive to one side of the connector and / or one side of the mounting part, attach the connector to one side of the mounting part, and after the adhesive has solidified, the connector and the mounting part are connected. Electrode wire welding steps: Pass one end of each electrode wire through the wire groove and weld each pin of the connector in turn. Electrode wire fixing steps: Apply adhesive to the position where each electrode wire passes through the corresponding mounting hole, or inject adhesive into each mounting hole. After the adhesive solidifies, each electrode wire will be fixedly connected to the corresponding mounting hole.

9. The method for preparing the electrode device for collecting electrical activity in brain regions as described in claim 8, characterized in that, The mounting part further includes a mounting chamber, and the mounting channel passes through the mounting chamber; The electrode wire installation constraint step further includes: before the wire bundle tube is installed into the sliding channel, a connector is placed in the installation cavity, and an adjusting member is rotatably installed in the installation part, one end of the adjusting member extending into the installation cavity along the extension direction of the sliding channel and threadedly connected to the connector; After the cable bundle is installed into the sliding channel, an adhesive is applied to the outer surface of the connector and / or the outer surface of the cable bundle. After the adhesive cures, the connector is bonded to the cable bundle.

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