A method for gradient temperature acclimation of primula plants
By using a gradient temperature acclimatization method, adjusting temperature and light conditions month by month, the problem of poor growth of Primula species in low-altitude areas was solved, achieving stable traits and efficient propagation, and promoting their horticultural application.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-05-12
- Publication Date
- 2026-07-31
AI Technical Summary
When plants of the Primula genus are directly transplanted from high-altitude, low-temperature environments to low-altitude, warmer regions, they experience poor growth, inhibited flowering, or even death. Current technologies lack effective methods for high-temperature adaptation and domestication, which limits their horticultural promotion.
A gradient temperature acclimatization method was adopted, in which the temperature was adjusted month by month from January to December in an artificial climate incubator, ranging from 1.8℃ to 22.9℃, combined with a light intensity of 3500 to 4500 Lux and a humidity of 65% ± 10%, to simulate changes in the natural environment and carry out full life history acclimatization.
This study has enabled the successful growth and propagation of Primula species in low-altitude areas, ensuring stable traits, controllable operation, clear cycle, and enhanced adaptability, thus providing technical support for industrial development.
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Figure CN122477913A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of high-altitude plant germplasm resource domestication and adaptive cultivation technology, and specifically relates to a gradient temperature domestication method for alpine Primula plants. Background Technology
[0002] Primula species are renowned alpine flowers, with the southwestern Yunnan mountains being one of their differentiation and distribution centers, possessing extremely rich wild germplasm resources. Many species of this genus are distributed in high-altitude, cold habitats above 3000 meters, and their long-term adaptation to low temperatures makes them particularly sensitive to warming. In introduction and domestication practices, directly transplanting high-altitude Primula plants to lower-altitude, warmer regions often leads to poor growth, inhibited flowering, and even death due to temperature increases. This problem severely restricts their horticultural promotion. Figure 1 .
[0003] Current research on the domestication of Primula species mainly focuses on systematic studies of seed germination and substrate selection, resulting in the large-scale propagation of seedlings for multiple species. The main focus is on optimizing cultivation substrates and conventional seedling techniques, without addressing specific designs for temperature domestication. In the field of temperature domestication, existing technologies largely concentrate on improving the cold tolerance of plants (cold domestication), with few reports on targeted domestication techniques for the high-temperature adaptability of alpine plants. Summary of the Invention
[0004] To address the aforementioned problems, the purpose of this invention is to provide a gradient temperature acclimatization method for Primula species, which enables Primula species to grow and reproduce well in warm, low-altitude regions.
[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows:
[0006] A gradient temperature acclimatization method for alpine Primula species includes the following steps: collecting seeds and placing them in an incubator for gradient temperature acclimatization; wherein the acclimatization temperature is 1.8℃~6.8℃ in January, 3.3℃~8.3℃ in February, 6.5℃~11.5℃ in March, 10.2℃~15.2℃ in April, 13.8℃~18.8℃ in May, and in June... The acclimatization temperatures were 16.5℃~21.5℃, 17.9℃~22.9℃ in July, 17.2℃~22.2℃ in August, 14.6℃~19.6℃ in September, 10.3℃~15.3℃ in October, 5.8℃~10.8℃ in November, and 2.5℃~7.5℃ in December, until P1 generation acclimatized seeds were obtained.
[0007] Preferably, the gradient temperature acclimatization is as follows: acclimatization temperature in January is 3.8℃, in February is 5.3℃, in March is 8.5℃, in April is 12.2℃, in May is 15.8℃, in June is 18.5℃, in July is 19.9℃, in August is 19.2℃, in September is 16.6℃, in October is 12.3℃, in November is 7.8℃, and in December is 4.5℃, until P1 generation acclimatized seeds are obtained.
[0008] Preferably, the Primula species is selected from Primula spp.
[0009] Preferably, the seeds are sourced from areas at an altitude of 3000 meters or higher.
[0010] Preferably, the gradient temperature acclimatization is carried out under the following conditions: 10 to 14 hours of light per day at a light intensity of 3500 to 4500 Lux, and a humidity of 65% ± 10%.
[0011] Particularly preferred is that the gradient temperature acclimatization is carried out under the following lighting conditions: 12 hours of illumination per day at a light intensity of 4000 Lux.
[0012] The present invention has the following beneficial effects:
[0013] (1) The domestication temperature setting is based on scientific evidence; the present invention sets different temperatures for the target species each month, thereby applying continuous high temperature selection pressure, making the domestication process more gentle and controllable.
[0014] (2) Full life cycle domestication ensures trait stability; This invention realizes continuous domestication from seed to seed throughout the entire life cycle, ensuring that plants experience temperature selection pressure during morphogenesis, physiological metabolism and reproduction.
[0015] (3) The operation is controllable and the cycle is clear. The entire process is carried out in an artificial climate incubator, and the environmental conditions are precisely controllable. The domestication cycle is consistent with the life cycle of Primula plants, providing an efficient and reliable technical path for germplasm innovation and industrial development of alpine flowers. Attached Figure Description
[0016] Figure 1 Bar chart showing the effect of warming stress on the transplant mortality rate of Primula seedlings;
[0017] Figure 2 Photographs of Primula vulgaris seeds germinating in Generation P1;
[0018] Figure 3 A bar chart showing the survival rate of P1 generation Primula vulgaris seedlings after transplanting;
[0019] Figure 4 Photograph of Primula vulgaris P1 growing at room temperature at an altitude of 1650 meters;
[0020] Figure 5 This is a photo of Primula vulgaris P1 growing and flowering normally at room temperature at an altitude of 1650 meters. Detailed Implementation
[0021] The specific embodiments of the present invention will be further described below with reference to the accompanying drawings. It should be noted that these descriptions are for the purpose of aiding understanding the present invention, but do not constitute a limitation thereof. Furthermore, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.
[0022] Example 1
[0023] A gradient temperature acclimatization method for alpine Primula species includes the following steps:
[0024] (1) Material collection
[0025] In a high-altitude meadow at 3010 meters above sea level in Longyang District, Yunnan Province, a natural population of wild Primula denticulata was selected. In May 2021, during the seed maturity period, more than 1000 seeds (P0 generation) of wild plants were collected, along with 50 kg of the top 0-10 cm of native soil. The mixture was then stored at 4℃ for later use.
[0026] (2) Gradient temperature acclimatization culture
[0027] In January 2022, native soil was divided into 20 seedling pots (12cm in diameter), with 10 P0 generation seeds sown in each pot. These were placed in an RXZ programmable artificial climate incubator. The acclimatization temperatures were set as follows: January 3.8℃, February 5.3℃, March 8.5℃, April 12.2℃, May 15.8℃, June 18.5℃, July 19.9℃, August 19.2℃, September 16.6℃, October 12.3℃, November 7.8℃, and December 4.5℃. Light conditions included 12 hours of light per day at 4000 Lux, humidity of 65%±10%, and regular watering to keep the soil moist. Continue cultivating until it blooms the following year.
[0028] (3) Artificial pollination and domestication of seed harvest
[0029] The plants entered their peak flowering period in March of the year following sowing (March 2023). Artificial pollination was used, employing a clean brush to collect pollen for cross-pollination on the same plant. The capsules were harvested in early May when the top split open, and the seeds were dried in the shade to obtain P1 generation domesticated seeds.
[0030] Example 2
[0031] A gradient temperature acclimatization method for alpine Primula species includes the following steps:
[0032] (1) Material collection
[0033] In a high-altitude meadow at 3010 meters above sea level in Longyang District, Yunnan Province, a natural population of wild Primula denticulata was selected. In May 2021, during the seed maturity period, more than 1000 seeds (P0 generation) of wild plants were collected, along with 50 kg of the top 0-10 cm of native soil. The mixture was then stored at 4℃ for later use.
[0034] (2) Gradient temperature acclimatization culture
[0035] In January 2022, native soil was divided into 20 seedling pots (12cm in diameter), with 10 P0 generation seeds sown in each pot. These were placed in an RXZ programmable artificial climate incubator. The acclimatization temperatures were set as follows: January 1.8℃, February 3.3℃, March 6.5℃, April 10.2℃, May 13.8℃, June 16.5℃, July 17.9℃, August 17.2℃, September 14.6℃, October 10.3℃, November 5.8℃, and December 2.5℃. Light conditions included 10 hours of light per day at 3500 Lux, humidity of 65%±10%, and regular watering to keep the soil moist. Continue cultivating until it blooms the following year.
[0036] (3) Artificial pollination and domestication of seed harvest
[0037] The plants entered their peak flowering period in March of the year following sowing (March 2023). Artificial pollination was used, employing a clean brush to collect pollen for cross-pollination on the same plant. The capsules were harvested in early May when the top split open, and the seeds were dried in the shade to obtain P1 generation domesticated seeds.
[0038] Example 3
[0039] A gradient temperature acclimatization method for alpine Primula species includes the following steps:
[0040] (1) Material collection
[0041] In a high-altitude meadow at 3010 meters above sea level in Longyang District, Yunnan Province, a natural population of wild Primula denticulata was selected. In May 2021, during the seed maturity period, more than 1000 seeds (P0 generation) of wild plants were collected, along with 50 kg of the top 0-10 cm of native soil. The mixture was then stored at 4℃ for later use.
[0042] (2) Gradient temperature acclimatization culture
[0043] In January 2022, native soil was divided into 20 seedling pots (12cm in diameter), with 10 P0 generation seeds sown in each pot. These were placed in an RXZ programmable artificial climate incubator. The acclimatization temperatures were set as follows: January 6.8℃, February 8.3℃, March 11.5℃, April 15.2℃, May 18.8℃, June 21.5℃, July 22.9℃, August 22.2℃, September 19.6℃, October 15.3℃, November 10.8℃, and December 7.5℃. Light conditions included 14 hours of light per day at 4500 Lux, humidity of 65%±10%, and regular watering to keep the soil moist. Continue cultivating until it blooms the following year.
[0044] (3) Artificial pollination and domestication of seed harvest
[0045] The plants entered their peak flowering period in March of the year following sowing (March 2023). Artificial pollination was used, employing a clean brush to collect pollen for cross-pollination on the same plant. The capsules were harvested in early May when the top split open, and the seeds were dried in the shade to obtain P1 generation domesticated seeds.
[0046] To verify the technical effects of the present invention, the following experiments were conducted:
[0047] Ninety seeds each of the P0 native habitat seeds and the P1 domesticated seeds obtained in Example 1 were placed in petri dishes lined with moistened filter paper, 30 seeds per dish, and germination was tested in a constant temperature incubator at 20℃ (12 hours of light / day). After 30 days of cultivation, the germination rate was calculated: the average germination rate of the P1 generation was 87.4% ± 3.1% (see...). Figure 2 The average germination rate of the P0 generation was 36.2% ± 3.3%.
[0048] Seedlings were transplanted into seedling trays containing native soil and placed at an altitude of 1650 meters outside the laboratory (temperature 20-25℃). The soil was kept moist, and seedling growth was observed after 30 days. Most P0 generation seedlings showed yellowing leaves and significantly slowed growth. P1 generation seedlings had fully expanded leaves, normal leaf color, and eventually flowered and fruited normally. Three transplanting tests were conducted, and the seedling survival rate reached 93% ± 3.6% (see...). Figure 3-5 ).
[0049] The embodiments of the present invention have been described in detail above, but the present invention is not limited to the described embodiments. For those skilled in the art, various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and these variations still fall within the protection scope of the present invention.
Claims
1. A gradient temperature acclimatization method for alpine Primula species, characterized in that: Includes the following steps: Seeds were collected and placed in an incubator for gradient temperature acclimatization. The acclimatization temperatures were as follows: January 1.8℃~6.8℃, February 3.3℃~8.3℃, March 6.5℃~11.5℃, April 10.2℃~15.2℃, May 13.8℃~18.8℃, June 16.5℃~21.5℃, July 17.9℃~22.9℃, August 17.2℃~22.2℃, September 14.6℃~19.6℃, October 10.3℃~15.3℃, November 5.8℃~10.8℃, and December 2.5℃~7.5℃, until P1 generation acclimatized seeds were obtained.
2. The gradient temperature acclimatization method for alpine Primula plants according to claim 1, characterized in that, The gradient temperature acclimatization was as follows: January acclimatization temperature was 3.8℃, February acclimatization temperature was 5.3℃, March acclimatization temperature was 8.5℃, April acclimatization temperature was 12.2℃, May acclimatization temperature was 15.8℃, June acclimatization temperature was 18.5℃, July acclimatization temperature was 19.9℃, August acclimatization temperature was 19.2℃, September acclimatization temperature was 16.6℃, October acclimatization temperature was 12.3℃, November acclimatization temperature was 7.8℃, and December acclimatization temperature was 4.5℃, until P1 generation acclimatized seeds were obtained.
3. A gradient temperature acclimatization method for alpine Primula plants according to claim 1 or 2, characterized in that, The plants in the Primula genus mentioned are selected from Primula gloriosa.
4. A gradient temperature acclimatization method for alpine Primula plants according to claim 1 or 2, characterized in that, The seeds were sourced from areas at an altitude of over 3,000 meters.
5. A gradient temperature acclimatization method for alpine Primula plants according to claim 1 or 2, characterized in that, The gradient temperature acclimatization was carried out under the following conditions: light intensity of 3500-4500 Lux for 10-14 hours per day, and humidity of 65%±10%.
6. The gradient temperature acclimatization method for alpine Primula plants according to claim 5, characterized in that, The gradient temperature acclimatization was carried out under the following lighting conditions: 12 hours of illumination per day at a light intensity of 4000 Lux.