A cultivation device and method for hydroponic-soil conversion of Fritillaria thunbergii.

Abstract

This invention provides a cultivation device and method for the hydroponic-soil conversion of Fritillaria thunbergii, belonging to the field of agricultural cultivation. The cultivation device includes a protective net bag made of biodegradable material, a fine fibrous, water-retaining, and breathable inner substrate filling the net bag, and a transition substrate block with a central groove. The cultivation method involves wrapping the bulbs and roots of hydroponic seedlings in the inner substrate and placing them in the protective net bag, then placing them in the groove of the transition substrate block to harden them off. Once the roots have emerged from the net bag, the entire assembly is buried in field soil for natural degradation. This invention shortens the seedling establishment period and the entire growth cycle through a multi-stage media transition of "aquatic roots - coconut coir microenvironment - transition substrate block - soil." Simultaneously, the device completely degrades in the soil within 3-6 months, eliminating the need for manual recycling and significantly reducing transplanting damage and maintenance costs.

Description

Technical Field

[0001] This invention relates to the field of agricultural cultivation, specifically to a cultivation device and method for converting Zhejiang fritillary bulbs from hydroponics to soil cultivation. Background Technology

[0002] Traditional cultivation of Fritillaria thunbergii primarily relies on soil or substrate. Fritillaria thunbergii planted before the Mid-Autumn Festival typically develops roots before sprouting and grows normally; however, those planted in November tend to sprout first and then develop roots, resulting in poorer growth. We address this issue by using hydroponics to promote root development. Hydroponic seedling cultivation technology has gained increasing attention due to its advantages such as controllable environment, induced rooting, and rapid growth. However, a significant "root adaptation gap" exists between hydroponics and soil cultivation, and the survival rate and maturity efficiency during transplantation remain core bottlenecks hindering production practices. Roots developed under hydroponic conditions are called "aquatic roots," characterized by thin-walled cells, well-developed air cavities, thin cell walls, and a lack of mechanical strength. When hydroponically grown seedlings are directly transplanted into soil, aquatic roots struggle to adapt to the friction, oxygen deficiency, and invasion of soil microorganisms, often exhibiting prolonged recovery periods, root rot, plant wilting, and even death.

[0003] To address this issue, various transitional solutions have been developed in existing technologies. For example, for floating seedling cultivation of chili peppers, a water-freeing and hardening-off process is required before transplanting—the water level is lowered to one-third of the root system, and water is gradually controlled 7 days in advance, combined with the application of rooting agents. This can shorten the seedling recovery period to 6.8 days and increase the survival rate to 95.5%. Other technologies address this from an equipment perspective. For instance, patent CN218526915U proposes a combined hydroponic and soil-based seedling cultivation device. By setting up seedling pots and telescopic rods within an observation glass box, the roots are pruned during the transition from hydroponics to soil cultivation, reducing the need for manual movement and positioning. Furthermore, in recent years, integrated "dual-cultivation" equipment has emerged, combining substrate cultivation and hydroponics, attempting to bridge the gap between the two modes within the cultivation process itself. In the field of Fritillaria thunbergii, the hydroponic seedling technology of Fritillaria thunbergii has achieved initial success under laboratory conditions—achieving rapid propagation and expanding the number of annual production seasons from one season to a theoretical three-round cultivation throughout the year; however, the bulbs still need to be grown in soil afterward, and the connection between hydroponics and soil cultivation remains a bottleneck that limits large-scale application.

[0004] The aforementioned existing technologies have the following defects and shortcomings: (1) Existing seedling hardening techniques mostly rely on environmental control (water cut-off, humidity control, shading) and chemical methods (rooting agent treatment). The operation process is complicated, the management standards are difficult to unify, and there are two-way operation risks of "excessive water control - root dehydration and death" and "insufficient water control - root system is still brittle and tender". The technical level of the operators is high, and it is difficult to promote and apply stably in large-scale scenarios such as fields, municipalities, and courtyards, which is not conducive to the promotion of the industry.

[0005] (2) Although the combined incubator achieves “water-soil symbiosis” at the equipment level, it is based on the continuous supply of nutrient solution and artificial root pruning, which fails to fundamentally change the fragile adaptation of hydroponic seedlings to the solid environment. Moreover, the equipment that needs to be continuously operated keeps the system cost high.

[0006] (3) In the root planting stage, the existing practices generally require manual dismantling of the protective structure or secondary transplantation, which increases the operation steps and brings additional mechanical damage risks.

[0007] Because Fritillaria thunbergii has a bulbous structure, a long growth cycle, and is sensitive to root integrity, the adverse effects caused by the above-mentioned deficiencies are particularly prominent, making it difficult to meet the production requirements of rapid molding while ensuring survival rate.

[0008] Therefore, there is an urgent need for a structurally integrated, simplified, and scalable hydroponic-soil conversion scheme for Fritillaria thunbergii to improve transplant survival rate, shorten the entire cycle time from seedling to bulb formation, and achieve efficient production and low-cost maintenance. Summary of the Invention

[0009] To address the technical problems of existing methods for inducing root growth in hydroponically grown Fritillaria thunbergii, such as the inability of the root system to directly adapt to the soil and the cumbersome operation, this invention provides a structurally integrated and easy-to-operate hydroponic-soil conversion cultivation device and cultivation method for Fritillaria thunbergii.

[0010] The core concept of the technical solution adopted in this invention is as follows: A cultivation device for hydroponic-soil conversion of Fritillaria thunbergii, comprising: The protective netting is made of biodegradable material and has multiple openings on its sidewalls for roots to pass through. The inner matrix filling the inside of the protective net bag is used to wrap the bulb and root system of the Fritillaria thunbergii seedlings; the inner matrix is ​​a fine fibrous water-retaining and breathable material. And a transition substrate block having a central groove that matches the shape of the protective net, the protective net being detachably placed in the central groove.

[0011] In one embodiment, the protective net is made of corn starch-based biodegradable plastic, with a porosity of 50% to 80% and a pore size of 2 to 5 mm.

[0012] In one embodiment, the inner matrix is ​​coconut coir fiber with a fiber fineness of ≤0.5mm, and has undergone high-temperature sterilization treatment.

[0013] In one embodiment, the transition matrix block is formed by pressing the following raw materials in parts by weight: 50-70 parts rice straw powder, 15-35 parts decomposed organic matter, 1-3 parts calcium oxide, 2-3 parts magnesium oxide, 3-5 parts polyacrylamide water-retaining agent, and 4-7 parts starch binder.

[0014] In one embodiment, the transition matrix block has a cuboid structure.

[0015] This invention also provides a cultivation method for hydroponic-soil conversion of Fritillaria thunbergii, which uses the cultivation device described in any of the above-mentioned embodiments and includes the following steps: Step 1: Wrap the bulbs and roots of the hydroponically grown Fritillaria thunbergii seedlings with the inner substrate and then place them into the protective net bag. Step 2: Place the protective net bag containing the seedlings in the central groove of the transition substrate block and cultivate it in a hardening environment until the roots penetrate through the holes of the protective net bag and extend into the transition substrate block. Step 3: Bury the combined device obtained in Step 2 into the field soil or cultivation container, allowing the protective net and transition substrate block to degrade naturally in the soil.

[0016] As one implementation method, the seedling hardening environment in step 2 is: temperature 20℃±1℃, relative humidity 70%~90%, light intensity 30~50μmol / m²·s, and cultivation time 7~14 days.

[0017] In one embodiment, the Zhejiang fritillary bulb is either Zhejiang fritillary bulb No. 1 or Zhejiang fritillary bulb No. 3.

[0018] Compared with the prior art, the present invention has the following beneficial effects: 1. This invention first utilizes hydroponics for factory-scale seedling cultivation, optimizing the nutrient solution formula and light and temperature conditions in a controlled environment, significantly accelerating the early growth rate of *Fritillaria thunbergii* compared to traditional soil-based seedling cultivation. Simultaneously, the seamless connection between the protective netting and the transition substrate blocks eliminates the lengthy acclimatization period of traditional transplanting, allowing the plants to quickly resume growth after planting. Experiments show that the total time from seedling cultivation to the formation of ideal bulbs is shortened.

[0019] 2. In the method of this invention, the hydroponic seedling stage is carried out in a completely controlled environment, resulting in clean and uniform seedlings. This is beneficial for the standardized construction and low-cost maintenance of Fritillaria thunbergii.

[0020] 3. Through the multi-level media transition path provided by this invention—"aquatic roots → coconut coir microenvironment → transition substrate block → field soil"—the tender aquatic roots gradually transform into more resilient and adaptable "transformed roots." This allows the plant to effectively adapt to the soil environment, maintains the bulb effect for a longer period, and eliminates the need for complex hardening-off operations or secondary transplanting throughout the process. This effectively avoids the damage to the root system caused by traditional hardening-off to transplanting stages, significantly reducing the difficulty and technical requirements of cultivation.

[0021] 4. This invention integrates root protection, medium transition, and planting fixation into a combined device consisting of a protective net bag, an inner substrate, and a transition substrate block. During planting, no parts need to be disassembled, and no secondary transplanting or manual retrieval is required; the entire device can be directly buried in the soil. This operation mode significantly reduces labor costs and is particularly suitable for large-scale or decentralized operations in fields, municipal areas, and courtyards, overcoming the bottleneck of traditional hardening-off methods being "cumbersome to operate and difficult to standardize."

[0022] 5. The protective netting (made of biodegradable plastic such as corn starch-based plastic) and the transition substrate blocks (made of straw, organic fertilizer, etc.) used in this invention can both naturally degrade in the soil within 3-6 months, leaving no environmental residue. During the degradation process, the organic matter released by the netting material and the nutrients produced by the decomposition of the substrate blocks can also provide slow-release nutrition for the later growth of the bulbs, forming a virtuous cycle of "device-soil-crop", avoiding the problems of traditional seedling containers needing to be recycled or leaving plastic pollution.

[0023] 6. This invention provides physical isolation and root guidance (i.e., mechanical protection) through a protective net bag, maintains humidity and aeration around the aquatic roots through the inner layer of coconut coir substrate (i.e., medium buffering), guides the roots to gradually adapt to the mechanical environment of the solid substrate through transition substrate blocks (i.e., transforming root cultivation), and eliminates secondary transplanting damage through the "zero disassembly and whole burial" planting method (i.e., operation damage reduction). The four elements work together to achieve a seamless transition from hydroponic seedlings to soil culture environment.

[0024] 7. More importantly, this invention increases the selectivity of crop rotation for Fritillaria thunbergii, such as rotation with watermelon, beans, and rice, thereby increasing the economic value per mu, increasing soil nitrogen fixation or disease control, which is conducive to realizing ecological planting and solving the pain points of the corresponding industries. Attached Figure Description

[0025] The accompanying drawings are provided to further understand the technical solutions of the present invention and constitute a part of the specification. They are used together with the embodiments of this application to explain the technical solutions of the present invention and do not constitute a limitation on the technical solutions of the present invention.

[0026] Figure 1 This is a structural diagram of the hydroponic-soil conversion cultivation device described in this invention.

[0027] Figure 2 yes Figure 1 A partial structural diagram of the structure shown.

[0028] Figure 3 This is a schematic flowchart of the method for cultivating Fritillaria thunbergii using hydroponics-soil conversion as described in this invention.

[0029] Figure 4 This is a diagram showing the growth of Fritillaria thunbergii after being treated by the cultivation method of this invention and then transferred to soil culture.

[0030] Marker explanation: 1-Transitional substrate block; 2-Central groove; 3-Fritillaria thunbergii; 4-Bulb; 5-Inner substrate; 6-Protective net. Detailed Implementation

[0031] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the described embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0032] Traditionally, Fritillaria thunbergii is planted from late September to early October, which encroaches on land used for rice or legume cultivation. Delaying the planting time to November allows for the harvesting of rice or legumes before planting Fritillaria thunbergii, potentially optimizing the entire planting system. This invention's technical solution can delay the planting time while ensuring normal growth, thereby improving land utilization and achieving a synergistic effect of ecological and economic value. For example, crop rotation with rice increases the crop season and improves land utilization; crop rotation with legumes allows for nitrogen fixation, improving soil quality and reducing nitrogen fertilizer application.

[0033] The transitional substrate block 1 used in this invention contains calcium and magnesium. During substrate block preparation and the initial hardening-off period, calcium and magnesium ions act as "bridges," connecting organic matter particles and mineral particles, or binding them together through coordination complexation to form an aggregate structure with a certain compressive strength. This structure significantly improves the water resistance and morphological stability of the substrate block during the hardening-off period (7-14 days), preventing it from softening and collapsing too quickly due to frequent watering, thus ensuring the fixation and protection of the root system during hardening-off. As the time after transplanting increases (more than 3 months), extracellular enzymes secreted by microorganisms widely present in the soil gradually break the polysaccharide bonds in the organic matter, starch binders and straw cellulose are slowly mineralized, and calcium and magnesium ions are released. The aggregate structure of the substrate block gradually disintegrates, eventually completely degrading into harmless soil components within 3-6 months, requiring no manual recycling.

[0034] The "seedling establishment period" refers to the time after transplanting when the seedlings resume growth and develop new roots, which is recorded through field observation. In this embodiment of the invention, new roots emerged from the netting and took root in the substrate within 7 days after the hydroponic seedlings were planted, indicating that they had completed the transformation of root function. Therefore, the actual seedling establishment period is within the range of 3 to 7 days.

[0035] The corn starch-based biodegradable plastic used in this invention is made from corn as a base material, supplemented with bio-polyester, polyols, and other substances. It is biodegradable in soil and natural environments, causing no pollution or harm to the environment. All its hygienic and physicochemical indicators meet national standards. It can be directly purchased from the market.

[0036] The materials used in the following examples of the present invention are commercially available conventional products that can be purchased through commercial channels by those skilled in the art.

[0037] Example 1 1. Material Preparation Tested variety: Fritillaria thunbergii No. 1, hydroponically cultured for 25 days, root length 3-5cm, number of roots 12-18.

[0038] Protective Net Bag 6: Made of corn starch-based biodegradable plastic, cylindrical, 5cm high, 4cm in diameter, with diamond-shaped pores on the side wall, porosity 50%, and pore diameter 3mm.

[0039] Inner matrix 5: extremely fine coconut coir fiber, fiber length ≤2mm, fineness ≤0.3mm, sterilized at 121℃ for 30 minutes and dried to a moisture content of 20%.

[0040] Transitional substrate block 1: The formula (by weight) is 70 parts rice straw powder, 15 parts well-rotted organic matter, 3 parts calcium oxide, 2 parts magnesium oxide, 3 parts soil conditioner (polyacrylamide-type water-retaining agent), and 7 parts starch binder. After mixing with water, it is pressed into a rectangular block of 25cm × 8cm × 5cm, with a working pressure of 15-30. The pressure is applied at 90-120 seconds, and the substrate is molded in a groove mold with the groove size matching the outer diameter of the mesh bag and a depth of 3 cm. It is then dried at 60℃ to a constant weight. In this embodiment, after pressing, the substrate blocks have a compressive strength of 0.8-1.2 MPa and a porosity of 55%-65%. They maintain their intact shape and do not collapse even after 7 days of hardening off.

[0041] The nutrient solution formula for hydroponics is as follows: nitrogen, phosphorus, and potassium in a volume ratio of 3:1:4, with a total concentration of 500 mg / L. Micronutrients are added in chelated form, including EDTA-Fe 80 g / L, ZnSO4 30 g / L, and H3BO3 5 g / L. Additionally, 5% compound amino acid solution and 2% seaweed extract are added. The pH is adjusted to 5.5–6.5 using an acid-base buffer, and finally, deionized water is used to bring the volume to 1000 mL. Continuous aeration with an air pump is used to maintain the dissolved oxygen concentration at 4–5 mg / L.

[0042] 2. Operating Procedures Hydroponic stress resistance induction: On the 20th day of hydroponics, dilute the nutrient solution to 50% of the initial concentration and stop aeration for 3 days (i.e., the 20th-22nd day of hydroponics) to induce the root system to enhance stress resistance.

[0043] Root wrapping: Take hydroponic fritillary bulb seedlings, separate the roots with tweezers, and wrap them evenly with about 30g of wet coconut coir fiber, so that the base of the bulb 4 is completely covered and the space between the roots is filled with fiber. Then gently place them into a protective net bag 6 and tie the opening of the net bag tightly with cotton rope.

[0044] Hardening off seedlings: Embed the prepared net bags into the central groove 2 of the transition substrate block 1, assemble, and place in an artificial climate chamber. Environmental parameters: temperature 20℃±1℃, relative humidity 70%~90%, light intensity 30~50μmol / m² 2 •s, photoperiod 12h / 12h. Spray daily to keep the surface of transition substrate block 1 moist but not waterlogged. After 8 days of hardening off, white root tips were observed to emerge from the mesh of the seedling bag and penetrate into the interior of transition substrate block 1 (average root extension coefficient 5.2 roots / plant), with a root length of 1-3cm.

[0045] Field planting: In the field, dig holes with a spacing of 30cm x 20cm and a depth of 7cm. Place the entire assembly into the hole, ensuring the top surface of the transition substrate block 1 is level with the top of the hole. Backfill with field soil, gently compact it, and water thoroughly. For the first week after planting, spray water once every evening, then switch to regular irrigation management.

[0046] Example 2 1. Material Preparation Tested variety: Fritillaria thunbergii No. 1, hydroponically cultured for 25 days, root length 3-5cm, number of roots 12-18.

[0047] Protective Net Bag 6: Made of corn starch-based biodegradable plastic, cylindrical, 5cm high, 4cm in diameter, with diamond-shaped pores on the side wall, porosity of 80%, and pore diameter of 3mm.

[0048] Inner matrix 5: extremely fine coconut coir fiber, fiber length ≤2mm, fineness ≤0.3mm, sterilized at 121℃ for 30 minutes and dried to a moisture content of 20%.

[0049] Transitional substrate block 1: The formula (by weight) is 50 parts rice straw powder, 35 parts decomposed organic matter, 1 part calcium oxide, 3 parts magnesium oxide, 5 parts soil conditioner (polyacrylamide-type water-retaining agent), and 5 parts starch binder. After mixing with water, it is pressed into a 25cm×8cm×5cm cuboid, shaped in a groove mold, with the groove size matching the outer diameter of the mesh bag and a depth of 3cm. It is then dried at 60℃ to a constant weight.

[0050] The nutrient solution formula for hydroponics is as follows: nitrogen, phosphorus, and potassium in a volume ratio of 3:1:4, with a total concentration of 500 mg / L. Micronutrients are added in chelated form, including EDTA-Fe 80 g / L, ZnSO4 30 g / L, and H3BO3 5 g / L. Additionally, 5% compound amino acid solution and 2% seaweed extract are added. The pH is adjusted to 5.5–6.5 using an acid-base buffer, and finally, deionized water is used to bring the volume to 1000 mL. Continuous aeration with an air pump is used to maintain the dissolved oxygen concentration at 4–5 mg / L.

[0051] 2. Operating Procedures Hydroponic stress resistance induction: On the 20th day of hydroponics, dilute the nutrient solution to 50% of the initial concentration and stop aeration for 3 days (i.e., the 20th-22nd day of hydroponics) to induce the root system to enhance stress resistance.

[0052] Root wrapping: Take hydroponic fritillary bulb seedlings, separate the roots with tweezers, and wrap them evenly with about 30g of wet coconut coir fiber, so that the base of the bulb 4 is completely covered and the space between the roots is filled with fiber. Then gently place them into a protective net bag 6 and tie the opening of the net bag tightly with cotton rope.

[0053] Hardening off seedlings: Embed the prepared net bags into the central groove 2 of the transition substrate block 1, assemble, and place in an artificial climate chamber. Environmental parameters: temperature 20℃±1℃, relative humidity 70%~90%, light intensity 30~50μmol / m² 2 •s, photoperiod 12h / 12h. Spray daily to keep the surface of transition substrate block 1 moist but not waterlogged. After 8 days of hardening off, white root tips were observed to emerge from the mesh of the seedling bag and penetrate into the interior of transition substrate block 1 (average root extension coefficient 5.2 roots / plant), with a root length of 1-3cm.

[0054] Field planting: In the field, dig holes with a spacing of 30cm x 20cm and a depth of 7cm. Place the entire assembly into the hole, ensuring the top surface of the transition substrate block 1 is level with the top of the hole. Backfill with field soil, gently compact it, and water thoroughly. For the first week after planting, spray water once every evening, then switch to regular irrigation management.

[0055] Comparative Example 1 1. Material Preparation Tested variety: Zhejiang Fritillaria No. 1. Four healthy, disease-free, and uniformly sized commercial bulbs were selected. The bulbs were disinfected by soaking in an 800-fold dilution of 50% carbendazim wettable powder for 30 minutes and then air-dried for later use.

[0056] Field soil: The previous crop was rice, the soil type is loam, pH 6.2, and organic matter content is 2.1%. When preparing the land, apply 2000 kg of well-rotted farmyard manure and 50 kg of compound fertilizer (N:P2O5:K2O=15:15:15) per mu as base fertilizer, deep plow to 25 cm, harrow and level, and make beds 1.2 m wide and 15 cm high.

[0057] 2. Operating Procedures Sowing: In late September of the same year, sow furrows 20cm apart and 8cm deep. Place the sterilized seed bulbs (4 bulbs) in the furrows at 15cm intervals, with the terminal bud facing upwards, cover with 5cm of soil, lightly press down, and water thoroughly. After sowing, cover the seedbed with 2cm of straw to retain moisture and prevent frost damage. The experiment had 3 replicate plots.

[0058] Winter management: Keep the furrows covered with straw during winter, and temporarily cover with plastic film to prevent freezing in extreme low temperatures.

[0059] Post-emergence management: Seedlings emerge in mid-to-late February of the following year; remove the straw. In early March, apply 10 kg of urea per mu to promote seedling growth. In early April, the bulbs enter the fourth bulb enlargement stage; apply 15 kg of potassium sulfate per mu, keep the soil moist, and clear ditches for drainage during the rainy season.

[0060] Harvesting: In mid-May of the following year, after the above-ground leaves naturally turn yellow and wither, select a sunny day to manually dig up 4 bulbs, harvest them separately according to the plot, remove the soil and residual roots, and measure the content of the main components.

[0061] Comparative Example 2 1. Material Preparation Tested variety: Zhejiang Fritillaria No. 3, using 4 healthy, disease-free, and uniformly sized commercial bulbs. The bulbs were disinfected by soaking in an 800-fold dilution of 50% carbendazim wettable powder for 30 minutes and then air-dried for later use.

[0062] Field soil: The previous crop was rice, the soil type is loam, pH 6.2, and organic matter content is 2.1%. When preparing the land, apply 2000 kg of well-rotted farmyard manure and 50 kg of compound fertilizer (N:P2O5:K2O=15:15:15) per mu as base fertilizer, deep plow to 25 cm, harrow and level, and make beds 1.2 m wide and 15 cm high.

[0063] 2. Operating Procedures Sowing: In late September of the same year, sow furrows 20cm apart and 8cm deep. Place the sterilized seed bulbs (4 bulbs) in the furrows at 15cm intervals, with the terminal bud facing upwards, cover with 5cm of soil, lightly press down, and water thoroughly. After sowing, cover the seedbed with 2cm of straw to retain moisture and prevent frost damage. The experiment had 3 replicate plots.

[0064] Winter management: Keep the furrows covered with straw during winter, and temporarily cover with plastic film to prevent freezing in extreme low temperatures.

[0065] Post-emergence management: Seedlings emerge in mid-to-late February of the following year; remove the straw. In early March, apply 10 kg of urea per mu to promote seedling growth. In early April, the bulbs enter the fourth bulb enlargement stage; apply 15 kg of potassium sulfate per mu, keep the soil moist, and clear ditches for drainage during the rainy season.

[0066] Harvesting: In mid-May of the following year, after the above-ground leaves naturally turn yellow and wither, select a sunny day to manually dig up 4 bulbs, harvest them separately according to the plot, remove the soil and residual roots, and measure the content of the main components.

[0067] Effect test After the Zhejiang fritillaria bulbs of Examples 1-2 and Comparative Examples 1-2 entered the harvest period, their average fresh weight, harvest survival rate, bulb diameter, and fritillarin content were tested.

[0068] Method for measuring average fresh weight: During the determined harvest period, a certain number of representative fritillaria bulbs are randomly selected from the sample. After sampling, they need to be processed as soon as possible, washed with clean water to remove impurities such as mud and sand, and dried on the surface. Weigh them using an electronic balance, add up the fresh weights of all the individual bulbs, and divide by the total number of bulbs.

[0069] Method for measuring harvest survival rate: During the harvest period, count the number of surviving fritillaria bulbs with intact bulbs, and calculate the harvest survival rate according to the following formula.

[0070] Harvest survival rate = (Number of surviving plants / Total number of plants) × 100% Method for measuring bulb diameter: After the fresh weight is weighed, use a ruler with an accuracy of 0.1 mm to measure the diameter at the widest point of the Fritillaria thunbergii bulb, and calculate the average bulb diameter.

[0071] For the determination method of fritillary alkaloids, please refer to: Che Peng, Liu Jiushi, Qi Yaodong, et al. Simultaneous determination of the contents of 6 alkaloids in Fritillaria cirrhosa by UPLC-ELSD[J]. Chinese Journal of Traditional Chinese Medicine, 2020, 45(6):1393-1398.

[0072] The test results are shown in Table 1-4.

[0073] Table 1

[0074] Table 2 Table 3

[0075] Table 4

[0076] While the embodiments disclosed in this invention are as described above, the content is merely for the purpose of facilitating understanding of the invention and is not intended to limit the invention. Any person skilled in the art to which this invention pertains may make any modifications and changes to the form and details of the implementation without departing from the spirit and scope disclosed herein; however, the scope of patent protection of this invention shall still be determined by the scope defined in the appended claims.

Claims

1. A cultivation device for water- soil conversion of Fritillaria thunbergii Miq., characterized in that, include: A protective net (6) made of biodegradable material has multiple openings on its sidewalls for roots to pass through; The inner matrix (5) filling the protective net bag (6) is used to wrap the bulb (4) and roots of the Fritillaria thunbergii (3) seedlings; the inner matrix (5) is a fine fibrous water-retaining and breathable material; And a transition substrate block (1), which has a central groove (2) that matches the shape of the protective net bag (6), and the protective net bag (6) is detachably placed in the central groove (2).

2. The rearing device of claim 1, wherein The protective net (6) is made of corn starch-based biodegradable plastic, with a porosity of 50%~80% and a pore size of 2~5mm.

3. The rearing device of claim 1, wherein The inner matrix (5) is coconut coir fiber with a fiber fineness of ≤0.5mm and has undergone high-temperature sterilization.

4. The rearing device of claim 1, wherein The transition matrix block (1) is formed by pressing the following raw materials in parts by weight: 50-70 parts of rice straw powder, 15-35 parts of decomposed organic matter, 1-3 parts of calcium oxide, 2-3 parts of magnesium oxide, 3-5 parts of polyacrylamide water-retaining agent, and 4-7 parts of starch binder.

5. The rearing device of claim 1, wherein The transition matrix block (1) has a cuboid structure.

6. A cultivation method for water culture-soil culture conversion of Fritillaria thunbergii Miq., characterized by, Using the cultivation apparatus according to any one of claims 1-5 includes the following steps: Step 1: Wrap the bulbs (4) and roots of the hydroponically grown Fritillaria thunbergii (3) seedlings with the inner substrate (5) and then put them into the protective net bag (6); Step 2: Place the protective net bag (6) containing the seedlings in the central groove (2) of the transition substrate block (1) and cultivate it in the hardening environment until the roots penetrate through the holes of the protective net bag (6) and extend into the transition substrate block (1); Step 3: Bury the combined device obtained in Step 2 into the field soil or cultivation container, so that the protective net (6) and the transition substrate block (1) can degrade naturally in the soil.

7. The breeding method according to claim 6, characterized by, The seedling hardening environment described in step 2 is as follows: temperature 20℃±1℃, relative humidity 70%~90%, light intensity 30~50μmol / m²·s, and cultivation time 7~14 days.

8. The breeding method according to claim 6, characterized by, The Zhejiang Fritillaria (3) is either Zhejiang Fritillaria No. 1 or Zhejiang Fritillaria No. 3.

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