A composite type regulator and its use in improving photosynthesis of crops

Abstract

This invention relates to a composite growth regulator and its use in improving crop photosynthesis, belonging to the technical field of plant growth regulators. The composite growth regulator of this invention mainly comprises heme or heme chloride, rhamnolipids, and choline chloride as its main active ingredients. The composite growth regulator provided by this invention has a broad resistance spectrum, which can improve crop photosynthesis under various adverse conditions such as high temperature and drought, efficiently repair and rapidly restore photosynthetic systems damaged by adverse conditions, and is suitable for use in various crops throughout their entire growth period.

Description

Technical Field

[0001] This invention belongs to the field of plant growth regulator technology, and relates to a compound regulator and its use in improving crop photosynthesis. Background Technology

[0002] Photosynthesis is the process by which higher green plants or photosynthetic autotrophic bacteria use light energy to convert carbon dioxide (CO2) and water into organic matter while releasing oxygen. It is the most important biochemical reaction on Earth. Optimizing photosynthesis is an effective way to further increase crop biomass and yield, and it is of great significance for solving global food security issues. Since photosynthesis is the foundation of crop yield, studying the mechanism of plant photosynthesis and its applications is an important means to improve agricultural production efficiency. Many factors affect the efficiency of crop photosynthesis, such as light duration, light area, light intensity, temperature, and carbon dioxide concentration. There are also many ways to improve photosynthetic efficiency, such as controlling environmental conditions in artificial greenhouses, discovering key stress-resistant genes, and cultivating high-light-efficiency plant varieties. In recent years, with technological breakthroughs, new strategies have emerged, such as using fluorescent carbon dots to regulate photosynthesis; and using synthetic biology to optimize carbon fixation systems, such as regulating photorespiration carbon flux and modifying Rubisco enzymes to create high-light-efficiency plants. In addition, introducing the C4 pathway into C3 plants to improve photosynthetic efficiency is also an important optimization direction, but it is complex to operate, costly, and has a narrow range of applications.

[0003] Furthermore, photosynthesis is the physiological basis for crop dry matter accumulation and yield formation. Under abiotic stresses such as drought, salinity, low temperature, and waterlogging, crop leaves experience a decrease in chlorophyll content, photosystem II is damaged by light inhibition, stomatal conductance is reduced leading to hindered CO2 absorption, and the activity of key carbon assimilation enzymes is inhibited, resulting in a significant reduction in photosynthetic efficiency and ultimately leading to crop yield reduction or even crop failure. Global climate change is causing more frequent extreme weather events, and agricultural production faces increasingly severe abiotic stress challenges. Developing plant regulators that can effectively maintain and improve crop photosynthetic efficiency under stress is of great significance for ensuring food security. Summary of the Invention

[0004] The main objective of this invention is to provide a composite growth regulator that can significantly improve the photosynthesis of crops under adverse conditions such as high temperature and drought, thus ensuring the growth needs of crops.

[0005] The present invention employs the following technical solutions to achieve the above objectives: A compound regulator, mainly containing the following components: Active ingredients, synergistic ingredients, auxiliary ingredients, and water.

[0006] Specifically, the active ingredient is mainly heme or heme chloride, and may also be compounded with other ingredients such as brassinolide and S-inducer. As a preferred method The active ingredients contain: 3-3.5 parts by weight of heme or heme chloride, 0.001-0.002 parts by weight of 28-homobrassinolide, and 0.01-0.03 parts by weight of spermine; Alternatively, the active ingredient may contain: 3-4 parts by weight of heme or heme chloride, 0.0005-0.0010 parts by weight of 14-hydroxysterol brassinolide, and 0.05-0.07 parts by weight of spermine; Alternatively, the active ingredient may contain: 3-3.5 parts by weight of heme or heme chloride, 0.0015-0.0018 parts by weight of S-inducer, and 0.02-0.03 parts by weight of betaine; Alternatively, the active ingredient may contain: 4-5 parts by weight of heme or heme chloride, 0.015-0.02 parts by weight of rhamnolipid, and 0.002-0.010 parts by weight of melatonin; Alternatively, the active ingredient may contain: 3-3.5 parts by weight of heme or heme chloride, 0.010-0.015 parts by weight of choline chloride, and 0.05-0.06 parts by weight of spermine.

[0007] As a preferred method The synergistic components include: 2-3 parts by weight of chitosan oligosaccharide, 3-6 parts by weight of purslane polysaccharide, 0.2-0.4 parts by weight of salicylic acid, 0.008-0.010 parts by weight of hydroxytyrosol, 0.1-0.2 parts by weight of isorhamnetin, 1-1.5 parts by weight of sodium hyaluronate, and 0.006-0.008 parts by weight of 5-aminolevulinic acid.

[0008] As a preferred method The auxiliary agent contains: 1-2 parts by weight of surfactant, 0.5-1 parts by weight of dispersant, and 1-2 parts by weight of trace element composition.

[0009] More preferably, the trace element composition is a mixture of ferric citrate, zinc sulfate, boric acid, and ammonium molybdate in a mass ratio of 2:0.5:1:1.

[0010] More preferably, the molecular weight of the chitosan oligosaccharide is 800~1500 Da.

[0011] More preferably, the purslane polysaccharide is prepared by adding commercially available purslane polysaccharide with a purity of 70% to an ethanol solution with a mass fraction of 80-90%, stirring, allowing to stand, filtering, and repeating the operation 2-3 times to purify the purslane polysaccharide.

[0012] More preferably, the surfactant includes, but is not limited to, fatty alcohol polyoxyethylene ethers, Tween surfactants, etc., mainly Tween surfactants, such as Tween-80.

[0013] More preferably, the dispersant includes, but is not limited to, lignin sulfonates, polymers, and alkyl sulfonates, such as sodium dodecyl sulfonate.

[0014] This invention provides a method for preparing the composite regulator, which mainly includes the following steps: Step 1: Preparation of the premixed solution of active ingredients The active ingredients are dissolved separately in water, ethanol, or ammonia, and then mixed to obtain a premixed solution of the active ingredients. Step 2: Preparation of the premixed solution of the synergistic ingredients Dissolve the synergistic ingredients separately in ethanol or water, mix them, and obtain a premixed solution of the synergistic ingredients; Step 3: Preparation of premixed solution of auxiliary agent components Dissolve the auxiliary components in water to obtain a premixed solution of the auxiliary components; Step 4: Preparation of the composite regulator While stirring, the premixed solution of active ingredients is slowly added to the premixed solution of synergistic ingredients, and stirred to form a homogeneous and transparent mixture. Then, while stirring, the premixed solution of adjuvant ingredients is slowly added to the homogeneous and transparent mixture, and stirring is continued. The pH is adjusted to 5.8-6.5, filtered, sterilized, dispensed into light-proof containers and sealed for storage to obtain the compound regulator.

[0015] This invention provides specific uses for the composite regulator, namely, the regulator can significantly improve crop photosynthesis, especially photosynthesis in adverse environments such as drought, high temperature, low temperature, and salinity, improve photosynthetic efficiency under adverse conditions, promote crop growth, and increase yield.

[0016] The present invention has the following beneficial effects: This invention employs a variety of plant regulators, with heme or heme chloride, choline chloride, rhamnolipids or brassinolide as the main active ingredients. These regulators activate multiple defense pathways within the plant, enhance photosynthetic enzyme activity, increase photosynthetic rate and stomatal conductance under stress, promote chlorophyll synthesis, and protect photosynthetic structures from stress damage. Simultaneously, purslane polysaccharides and salicylic acid are added as auxiliary synergists to help induce crop resistance to stress, prevent stomatal closure caused by stress, and ensure normal carbon dioxide absorption. The synergistic effects of these components construct a multi-dimensional formulation system encompassing "core repair, photosynthetic enhancement, conjugation protection, and nutrient supplementation," breaking through the limitations of single-regulator functions. This achieves multiple effects, including photosynthetic repair and conjugation enhancement under stress, significantly improving crop photosynthesis under adverse conditions such as high temperature, low temperature, drought, and low light. It is adaptable to various stress environments, provides comprehensive protection for the crop's photosynthetic system, and is suitable for all types of crops and can be used throughout the entire growth cycle. Detailed Implementation

[0017] The present invention is further illustrated below with reference to specific embodiments. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. After reading this invention, any modifications of the invention in various equivalent forms by those skilled in the art fall within the scope of protection of the claims of this application. The ingredients used in the following embodiments are all commercially available products, including hydroxytyrosol (also known as 3,4-dihydroxyphenylethanol), CAS number 10597-60-1; isorhamnetin, CAS number 480-19-3; and purslane polysaccharide, which is produced and sold by Shanxi Qiyuan Biotechnology Co., Ltd.

[0018] Example 1 Active ingredients: Heme 3 parts by weight, 28-homobrassinolide 0.002 parts by weight, spermine 0.03 parts by weight; Synergistic ingredients: 3 parts by weight of chitosan oligosaccharide, 6 parts by weight of purslane polysaccharide, 0.2 parts by weight of salicylic acid, 0.010 parts by weight of hydroxytyrosol, 0.1 parts by weight of isorhamnetin, 1.5 parts by weight of sodium hyaluronate, and 0.006 parts by weight of 5-aminolevulinic acid; Additive components: surfactant (Tween 80) 2 parts by weight, dispersant (sodium dodecyl sulfonate) 0.5 parts by weight, trace element composition (ferric citrate, zinc sulfate, boric acid, and ammonium molybdate mixed in a mass ratio of 2:0.5:1:1) 1 part by weight; Add deionized water to a total of 100 parts by weight; Preparation method: Step 1: Preparation of the premixed solution of active ingredients Spermine was added to 1 / 3 of the formula volume of deionized water and stirred until completely dissolved to obtain a spermine solution; heme was added to a small amount of 10% ammonia water and stirred until completely dissolved to obtain a heme solution; 28-homobrassinolide was added to a small amount of ethanol and stirred until completely dissolved, then added to the spermine solution, followed by the heme solution. The mixture was stirred at room temperature until all components were completely dissolved and mixed evenly to obtain a premixed solution of active ingredients. Step 2: Preparation of the premixed solution of the synergistic ingredients Add a small amount of ethanol to isorhamnetin and stir until completely dissolved to obtain an isorhamnetin ethanol solution; take 1 / 3 of the total mass of deionized water and heat to 30-35℃, add chitosan oligosaccharide, purslane polysaccharide, sodium hyaluronate and salicylic acid, stir until completely dissolved, and then cool to room temperature; after cooling, add hydroxytyrosol and 5-aminolevulinic acid in sequence, continue stirring until completely dissolved, add isorhamnetin ethanol solution, continue stirring until completely dissolved and mixed evenly to obtain a premixed solution of synergistic ingredients; Step 3: Preparation of premixed solution of auxiliary agent components Take the remaining 1 / 3 of the total mass of deionized water, add Tween-80 and sodium dodecylbenzenesulfonate, stir at 150-200 r / min until emulsified and uniform, then add the trace element composition, and continue stirring until completely dissolved to obtain the premixed solution of the auxiliary components; Step 4: Preparation of the composite regulator While stirring, slowly add the premixed solution of the active ingredient to the premixed solution of the synergistic ingredient, controlling the addition time at 5-8 minutes. After the addition is complete, continue stirring for 10-15 minutes to form a homogeneous and transparent mixture. Subsequently, continue stirring while slowly adding the premixed solution of the adjuvant ingredient to the homogeneous and transparent mixture, controlling the addition time at 3-5 minutes. After the addition is complete, continue stirring for 15-20 minutes. Adjust the pH to 5.8-6.5 using citric acid solution (10% concentration) or dilute sodium hydroxide solution (0.1 mol / L). Filter, sterilize, dispense into light-proof containers, seal and store to obtain the compound regulator.

[0019] Example 2 Active ingredients: Heme 3.5 parts by weight, 28-homobrassinolide 0.001 parts by weight, spermine 0.02 parts by weight; Synergistic ingredients: 2 parts by weight of chitosan oligosaccharide, 3 parts by weight of purslane polysaccharide, 0.4 parts by weight of salicylic acid, 0.008 parts by weight of hydroxytyrosol, 0.2 parts by weight of isorhamnetin, 1 part by weight of sodium hyaluronate, and 0.008 parts by weight of 5-aminolevulinic acid; Additive components: surfactant (Tween 80) 1 part by weight, dispersant (sodium dodecyl sulfonate) 1 part by weight, trace element composition (ferric citrate, zinc sulfate, boric acid, and ammonium molybdate mixed in a mass ratio of 2:0.5:1:1) 2 parts by weight; Add deionized water to a total of 100 parts by weight.

[0020] Preparation method: Refer to Example 1.

[0021] Example 3 Active ingredients: 4 parts by weight of heme chloride, 0.0005 parts by weight of 14-hydroxysterol brassinolide, and 0.05 parts by weight of spermine; Synergistic ingredients: 3 parts by weight of chitosan oligosaccharide, 4 parts by weight of purslane polysaccharide, 0.3 parts by weight of salicylic acid, 0.009 parts by weight of hydroxytyrosol, 0.1 parts by weight of isorhamnetin, 1.2 parts by weight of sodium hyaluronate, and 0.007 parts by weight of 5-aminolevulinic acid; Additive components: surfactant (Tween 80) 2 parts by weight, dispersant (sodium dodecyl sulfonate) 0.8 parts by weight, trace element composition (ferric citrate, zinc sulfate, boric acid, and ammonium molybdate mixed in a mass ratio of 2:0.5:1:1) 2 parts by weight; Add deionized water to a total of 100 parts by weight.

[0022] Preparation method: Step 1: Preparation of the premixed solution of active ingredients Spermine was added to 1 / 3 of the formula volume of deionized water and stirred until completely dissolved to obtain a spermine solution; heme chloride was added to a small amount of 10% ammonia water and stirred until completely dissolved to obtain a heme chloride solution; 14-hydroxysterol brassinolide was added to a small amount of ethanol and stirred until completely dissolved, then added to the spermine solution, followed by the heme chloride solution. The mixture was stirred at room temperature until all components were completely dissolved and mixed evenly to obtain a premixed solution of active ingredients. Step 2: Preparation of the premixed solution of the synergistic ingredients Add a small amount of ethanol to isorhamnetin and stir until completely dissolved to obtain an isorhamnetin ethanol solution; take 1 / 3 of the total mass of deionized water and heat to 30-35℃, add chitosan oligosaccharide, purslane polysaccharide, sodium hyaluronate and salicylic acid, stir until completely dissolved, and then cool to room temperature; after cooling, add hydroxytyrosol and 5-aminolevulinic acid in sequence, continue stirring until completely dissolved, add isorhamnetin ethanol solution, continue stirring until completely dissolved and mixed evenly to obtain a premixed solution of synergistic ingredients; Step 3: Preparation of premixed solution of auxiliary agent components Take the remaining 1 / 3 of the total mass of deionized water, add Tween-80 and sodium dodecylbenzenesulfonate, stir at 150-200 r / min until emulsified and uniform, then add the trace element composition, and continue stirring until completely dissolved to obtain the premixed solution of the auxiliary components; Step 4: Preparation of the composite regulator While stirring, slowly add the premixed solution of the active ingredient to the premixed solution of the synergistic ingredient, controlling the addition time at 5-8 minutes. After the addition is complete, continue stirring for 10-15 minutes to form a homogeneous and transparent mixture. Subsequently, continue stirring while slowly adding the premixed solution of the adjuvant ingredient to the homogeneous and transparent mixture, controlling the addition time at 3-5 minutes. After the addition is complete, continue stirring for 15-20 minutes. Adjust the pH to 5.8-6.5 using citric acid solution (10% concentration) or dilute sodium hydroxide solution (0.1 mol / L). Filter, sterilize, dispense into light-proof containers, seal and store to obtain the compound regulator.

[0023] Example 4: Active ingredients: Heme 3 parts by weight, 14-hydroxysterol brassinolide 0.0010 parts by weight, spermine 0.07 parts by weight; Synergistic ingredients: Chitosan oligosaccharide 2 parts by weight, Portulaca polysaccharide 6 parts by weight, salicylic acid 0.4 parts by weight, hydroxytyrosol 0.008 parts by weight, isorhamnetin 0.15 parts by weight, sodium hyaluronate 1.3 parts by weight, 5-aminolevulinic acid 0.006 parts by weight; Additive components: surfactant (Tween 80) 1 part by weight, dispersant (sodium dodecyl sulfonate) 1 part by weight, trace element composition (ferric citrate, zinc sulfate, boric acid, and ammonium molybdate mixed in a mass ratio of 2:0.5:1:1) 1.5 parts by weight; Add deionized water to a total of 100 parts by weight; Preparation method: Refer to Example 3.

[0024] Example 5 Active ingredients: Heme 3 parts by weight, S-inducer 0.0018 parts by weight, Betaine 0.03 parts by weight; Synergistic ingredients: 2.5 parts by weight of chitosan oligosaccharide, 4 parts by weight of purslane polysaccharide, 0.3 parts by weight of salicylic acid, 0.008 parts by weight of hydroxytyrosol, 0.2 parts by weight of isorhamnetin, 1 part by weight of sodium hyaluronate, and 0.008 parts by weight of 5-aminolevulinic acid; Additive components: surfactant (Tween 80) 1 part by weight, dispersant (sodium dodecyl sulfonate) 1 part by weight, trace element composition (ferric citrate, zinc sulfate, boric acid, and ammonium molybdate mixed in a mass ratio of 2:0.5:1:1) 1 part by weight; Add deionized water to a total of 100 parts by weight; Preparation method: Step 1: Preparation of the premixed solution of active ingredients Add betaine to 1 / 3 of the formula volume of deionized water and stir until completely dissolved to obtain a betaine solution; add heme to a small amount of 10% ammonia water and stir until completely dissolved to obtain a heme solution; add S-inducer to a small amount of ethanol and stir until completely dissolved, then add it to the spermine solution, then add the heme solution, and stir at room temperature until all components are completely dissolved and mixed evenly to obtain the active ingredient premixed solution; Step 2: Preparation of the premixed solution of the synergistic ingredients Add a small amount of ethanol to isorhamnetin and stir until completely dissolved to obtain an isorhamnetin ethanol solution; take 1 / 3 of the total mass of deionized water and heat to 30-35℃, add chitosan oligosaccharide, purslane polysaccharide, sodium hyaluronate and salicylic acid, stir until completely dissolved, and then cool to room temperature; after cooling, add hydroxytyrosol and 5-aminolevulinic acid in sequence, continue stirring until completely dissolved, add isorhamnetin ethanol solution, continue stirring until completely dissolved and mixed evenly to obtain a premixed solution of synergistic ingredients; Step 3: Preparation of premixed solution of auxiliary agent components Take the remaining 1 / 3 of the total mass of deionized water, add Tween-80 and sodium dodecylbenzenesulfonate, stir at 150-200 r / min until emulsified and uniform, then add the trace element composition, and continue stirring until completely dissolved to obtain the premixed solution of the auxiliary components; Step 4: Preparation of the composite regulator While stirring, slowly add the premixed solution of the active ingredient to the premixed solution of the synergistic ingredient, controlling the addition time at 5-8 minutes. After the addition is complete, continue stirring for 10-15 minutes to form a homogeneous and transparent mixture. Subsequently, continue stirring while slowly adding the premixed solution of the adjuvant ingredient to the homogeneous and transparent mixture, controlling the addition time at 3-5 minutes. After the addition is complete, continue stirring for 15-20 minutes. Adjust the pH to 5.8-6.5 using citric acid solution (10% concentration) or dilute sodium hydroxide solution (0.1 mol / L). Filter, sterilize, dispense into light-proof containers, seal and store to obtain the compound regulator.

[0025] Example 6 Active ingredients: 3 parts by weight of heme chloride, 0.0015 parts by weight of S-inducer, and 0.02 parts by weight of betaine; Synergistic ingredients: 3 parts by weight of chitosan oligosaccharide, 3 parts by weight of purslane polysaccharide, 0.4 parts by weight of salicylic acid, 0.010 parts by weight of hydroxytyrosol, 0.1 parts by weight of isorhamnetin, 1.5 parts by weight of sodium hyaluronate, and 0.006 parts by weight of 5-aminolevulinic acid; Additive components: surfactant (Tween 80) 2 parts by weight, dispersant (sodium dodecyl sulfonate) 0.5 parts by weight, trace element composition (ferric citrate, zinc sulfate, boric acid, and ammonium molybdate mixed in a mass ratio of 2:0.5:1:1) 2 parts by weight; Add deionized water to a total of 100 parts by weight; Preparation method: Refer to Example 5.

[0026] Example 7 Active ingredients: 5 parts by weight of heme chloride, 0.015 parts by weight of rhamnolipid, and 0.002 parts by weight of melatonin; Synergistic ingredients: 3 parts by weight of chitosan oligosaccharide, 5 parts by weight of purslane polysaccharide, 0.4 parts by weight of salicylic acid, 0.008 parts by weight of hydroxytyrosol, 0.2 parts by weight of isorhamnetin, 1 part by weight of sodium hyaluronate, and 0.006 parts by weight of 5-aminolevulinic acid; Additive components: surfactant (Tween 80) 2 parts by weight, dispersant (sodium dodecyl sulfonate) 0.5 parts by weight, trace element composition (ferric citrate, zinc sulfate, boric acid, and ammonium molybdate mixed in a mass ratio of 2:0.5:1:1) 2 parts by weight; Add deionized water to a total of 100 parts by weight; Preparation method: Step 1: Preparation of the premixed solution of active ingredients Melatonin was added to 1 / 3 of the formula volume of deionized water and stirred in a 45°C water bath until completely dissolved. The solution was then cooled to 30°C to obtain a melatonin solution. Heme chloride was added to a small amount of 10% ammonia water and stirred until completely dissolved to obtain a heme chloride solution. Rhamnolipid was added to a small amount of ethanol and stirred until completely dissolved. This solution was then added to the melatonin solution, followed by the heme chloride solution. The mixture was stirred at room temperature until all components were completely dissolved and homogeneous to obtain a premixed solution of the active ingredients. Step 2: Preparation of the premixed solution of the synergistic ingredients Add a small amount of ethanol to isorhamnetin and stir until completely dissolved to obtain an isorhamnetin ethanol solution; take 1 / 3 of the total mass of deionized water and heat to 30-35℃, add chitosan oligosaccharide, purslane polysaccharide, sodium hyaluronate and salicylic acid, stir until completely dissolved, and then cool to room temperature; after cooling, add hydroxytyrosol and 5-aminolevulinic acid in sequence, continue stirring until completely dissolved, add isorhamnetin ethanol solution, continue stirring until completely dissolved and mixed evenly to obtain a premixed solution of synergistic ingredients; Step 3: Preparation of premixed solution of auxiliary agent components Take the remaining 1 / 3 of the total mass of deionized water, add Tween-80 and sodium dodecylbenzenesulfonate, stir at 150-200 r / min until emulsified and uniform, then add the trace element composition, and continue stirring until completely dissolved to obtain the premixed solution of the auxiliary components; Step 4: Preparation of the composite regulator While stirring, slowly add the premixed solution of the active ingredient to the premixed solution of the synergistic ingredient, controlling the addition time at 5-8 minutes. After the addition is complete, continue stirring for 10-15 minutes to form a homogeneous and transparent mixture. Subsequently, continue stirring while slowly adding the premixed solution of the adjuvant ingredient to the homogeneous and transparent mixture, controlling the addition time at 3-5 minutes. After the addition is complete, continue stirring for 15-20 minutes. Adjust the pH to 5.8-6.5 using citric acid solution (10% concentration) or dilute sodium hydroxide solution (0.1 mol / L). Filter, sterilize, dispense into light-proof containers, seal and store to obtain the compound regulator.

[0027] Example 8 Active ingredients: Heme 4 parts by weight, rhamnolipid 0.02 parts by weight, melatonin 0.010 parts by weight; Synergistic ingredients: Chitosan oligosaccharide 2 parts by weight, Portulaca polysaccharide 6 parts by weight, salicylic acid 0.3 parts by weight, hydroxytyrosol 0.010 parts by weight, isorhamnetin 0.15 parts by weight, sodium hyaluronate 1.3 parts by weight, 5-aminolevulinic acid 0.006 parts by weight; Additive components: surfactant (Tween 80) 1.5 parts by weight, dispersant (sodium dodecyl sulfonate) 0.8 parts by weight, trace element composition (ferric citrate, zinc sulfate, boric acid, and ammonium molybdate mixed in a mass ratio of 2:0.5:1:1) 2 parts by weight; Add deionized water to a total of 100 parts by weight; Preparation method: Same as in Example 7.

[0028] Example 9 Active ingredients: 3.5 parts by weight of heme chloride, 0.010 parts by weight of choline chloride, and 0.05 parts by weight of spermine; Synergistic ingredients: 3 parts by weight of chitosan oligosaccharide, 4 parts by weight of purslane polysaccharide, 0.3 parts by weight of salicylic acid, 0.008 parts by weight of hydroxytyrosol, 0.1 parts by weight of isorhamnetin, 1.2 parts by weight of sodium hyaluronate, and 0.007 parts by weight of 5-aminolevulinic acid; Additive components: surfactant (Tween 80) 1.5 parts by weight, dispersant (sodium dodecyl sulfonate) 1 part by weight, trace element composition (ferric citrate, zinc sulfate, boric acid, and ammonium molybdate mixed in a mass ratio of 2:0.5:1:1) 2 parts by weight; Add deionized water to a total of 100 parts by weight; Preparation method: Step 1: Preparation of the premixed solution of active ingredients Spermine and choline chloride were added to 1 / 3 of the formula volume of deionized water and stirred until completely dissolved to obtain a mixed solution. Heme was added to a small amount of 10% ammonia water and stirred until completely dissolved. Then it was added to the above mixed solution and stirred at room temperature until all components were completely dissolved and mixed evenly to obtain the active ingredient premixed solution. Step 2: Preparation of the premixed solution of the synergistic ingredients Add a small amount of ethanol to isorhamnetin and stir until completely dissolved to obtain an isorhamnetin ethanol solution; take 1 / 3 of the total mass of deionized water and heat to 30-35℃, add chitosan oligosaccharide, purslane polysaccharide, sodium hyaluronate and salicylic acid, stir until completely dissolved, and then cool to room temperature; after cooling, add hydroxytyrosol and 5-aminolevulinic acid in sequence, continue stirring until completely dissolved, add isorhamnetin ethanol solution, continue stirring until completely dissolved and mixed evenly to obtain a premixed solution of synergistic ingredients; Step 3: Preparation of premixed solution of auxiliary agent components Take the remaining 1 / 3 of the total mass of deionized water, add Tween-80 and sodium dodecylbenzenesulfonate, stir at 150-200 r / min until emulsified and uniform, then add the trace element composition, and continue stirring until completely dissolved to obtain the premixed solution of the auxiliary components; Step 4: Preparation of the composite regulator While stirring, slowly add the premixed solution of the active ingredient to the premixed solution of the synergistic ingredient, controlling the addition time at 5-8 minutes. After the addition is complete, continue stirring for 10-15 minutes to form a homogeneous and transparent mixture. Subsequently, continue stirring while slowly adding the premixed solution of the adjuvant ingredient to the homogeneous and transparent mixture, controlling the addition time at 3-5 minutes. After the addition is complete, continue stirring for 15-20 minutes. Adjust the pH to 5.8-6.5 using citric acid solution (10% concentration) or dilute sodium hydroxide solution (0.1 mol / L). Filter, sterilize, dispense into light-proof containers, seal and store to obtain the compound regulator.

[0029] Example 10 Active ingredients: 3 parts by weight of heme, 0.015 parts by weight of choline chloride, and 0.06 parts by weight of spermine; Synergistic ingredients: 3 parts by weight of chitosan oligosaccharide, 5 parts by weight of purslane polysaccharide, 0.2 parts by weight of salicylic acid, 0.010 parts by weight of hydroxytyrosol, 0.1 parts by weight of isorhamnetin, 1 part by weight of sodium hyaluronate, and 0.006 parts by weight of 5-aminolevulinic acid; Additive components: surfactant (Tween 80) 2 parts by weight, dispersant (sodium dodecyl sulfonate) 0.5 parts by weight, trace element composition (ferric citrate, zinc sulfate, boric acid, and ammonium molybdate mixed in a mass ratio of 2:0.5:1:1) 2 parts by weight; Add deionized water to a total of 100 parts by weight; Preparation method: Same as in Example 9.

[0030] Comparative Example 1 Active ingredients: Heme 3 parts by weight, 28-homobrassinolide 0.002 parts by weight, spermine 0.03 parts by weight; Synergistic ingredients: 3 parts by weight of chitosan oligosaccharide, 0.2 parts by weight of salicylic acid, 0.1 parts by weight of isorhamnetin, 1.5 parts by weight of sodium hyaluronate, and 0.006 parts by weight of 5-aminolevulinic acid; Additive components: surfactant (Tween 80) 2 parts by weight, dispersant (sodium dodecyl sulfonate) 0.5 parts by weight, trace element composition (ferric citrate, zinc sulfate, boric acid, and ammonium molybdate mixed in a mass ratio of 2:0.5:1:1) 1 part by weight; Add deionized water to a total of 100 parts by weight; Preparation method: Step 1: Preparation of the premixed solution of active ingredients Spermine was added to 1 / 3 of the formula volume of deionized water and stirred until completely dissolved to obtain a spermine solution; heme was added to a small amount of 10% ammonia water and stirred until completely dissolved to obtain a heme solution; 28-homobrassinolide was added to a small amount of ethanol and stirred until completely dissolved, then added to the spermine solution, followed by the heme solution. The mixture was stirred at room temperature until all components were completely dissolved and mixed evenly to obtain a premixed solution of active ingredients. Step 2: Preparation of the premixed solution of the synergistic ingredients Add a small amount of ethanol to isorhamnetin and stir until completely dissolved to obtain an isorhamnetin ethanol solution; take 1 / 3 of the total mass of the formulation of deionized water and heat to 30~35℃, add chitosan oligosaccharide, sodium hyaluronate and salicylic acid, stir until completely dissolved, and then cool to room temperature; after cooling, add 5-aminolevulinic acid and continue stirring until completely dissolved, add isorhamnetin ethanol solution, and continue stirring until completely dissolved and mixed evenly to obtain a premixed solution of synergistic ingredients; Step 3: Preparation of premixed solution of auxiliary agent components Take the remaining 1 / 3 of the total mass of deionized water, add Tween-80 and sodium dodecylbenzenesulfonate, stir at 150-200 r / min until emulsified and uniform, then add the trace element composition, and continue stirring until completely dissolved to obtain the premixed solution of the auxiliary components; Step 4: Preparation of the composite regulator While stirring, slowly add the premixed solution of the active ingredient to the premixed solution of the synergistic ingredient, controlling the addition time at 5-8 minutes. After the addition is complete, continue stirring for 10-15 minutes to form a homogeneous and transparent mixture. Subsequently, continue stirring while slowly adding the premixed solution of the adjuvant ingredient to the homogeneous and transparent mixture, controlling the addition time at 3-5 minutes. After the addition is complete, continue stirring for 15-20 minutes. Adjust the pH to 5.8-6.5 using citric acid solution (10% concentration) or dilute sodium hydroxide solution (0.1 mol / L). Filter, sterilize, dispense into light-proof containers, seal and store to obtain the compound regulator.

[0031] Comparative Example 2 Active ingredients: 4 parts by weight of heme chloride, 0.0005 parts by weight of 14-hydroxysterol brassinolide, and 0.05 parts by weight of spermine; Synergistic ingredients: 3 parts by weight of chitosan oligosaccharide, 4 parts by weight of purslane polysaccharide, 0.009 parts by weight of hydroxytyrosol, 1.2 parts by weight of sodium hyaluronate, and 0.007 parts by weight of 5-aminolevulinic acid; Additive components: surfactant (Tween 80) 2 parts by weight, dispersant (sodium dodecyl sulfonate) 0.8 parts by weight, trace element composition (ferric citrate, zinc sulfate, boric acid, and ammonium molybdate mixed in a mass ratio of 2:0.5:1:1) 2 parts by weight; Add deionized water to a total of 100 parts by weight.

[0032] Preparation method: Step 1: Preparation of the premixed solution of active ingredients Spermine was added to 1 / 3 of the formula volume of deionized water and stirred until completely dissolved to obtain a spermine solution; heme chloride was added to a small amount of 10% ammonia water and stirred until completely dissolved to obtain a heme chloride solution; 14-hydroxysterol brassinolide was added to a small amount of ethanol and stirred until completely dissolved, then added to the spermine solution, followed by the heme chloride solution. The mixture was stirred at room temperature until all components were completely dissolved and mixed evenly to obtain a premixed solution of active ingredients. Step 2: Preparation of the premixed solution of the synergistic ingredients Take 1 / 3 of the total mass of the formula and heat it to 30~35℃. Add chitosan oligosaccharide, purslane polysaccharide and sodium hyaluronate, and stir until completely dissolved. Then cool to room temperature. After cooling, add hydroxytyrosol and 5-aminolevulinic acid in sequence, and continue stirring until completely dissolved to obtain the premixed solution of synergistic ingredients. Step 3: Preparation of premixed solution of auxiliary agent components Take the remaining 1 / 3 of the total mass of deionized water, add Tween-80 and sodium dodecylbenzenesulfonate, stir at 150-200 r / min until emulsified and uniform, then add the trace element composition, and continue stirring until completely dissolved to obtain the premixed solution of the auxiliary components; Step 4: Preparation of the composite regulator While stirring, slowly add the premixed solution of the active ingredient to the premixed solution of the synergistic ingredient, controlling the addition time at 5-8 minutes. After the addition is complete, continue stirring for 10-15 minutes to form a homogeneous and transparent mixture. Subsequently, continue stirring while slowly adding the premixed solution of the adjuvant ingredient to the homogeneous and transparent mixture, controlling the addition time at 3-5 minutes. After the addition is complete, continue stirring for 15-20 minutes. Adjust the pH to 5.8-6.5 using citric acid solution (10% concentration) or dilute sodium hydroxide solution (0.1 mol / L). Filter, sterilize, dispense into light-proof containers, seal and store to obtain the compound regulator.

[0033] Comparative Example 3 Active ingredients: Heme 3 parts by weight, S-inducer 0.0018 parts by weight, Betaine 0.03 parts by weight; Synergistic ingredients: 2.5 parts by weight of chitosan oligosaccharide and 0.008 parts by weight of 5-aminolevulinic acid; Additive components: surfactant (Tween 80) 1 part by weight, dispersant (sodium dodecyl sulfonate) 1 part by weight, trace element composition (ferric citrate, zinc sulfate, boric acid, and ammonium molybdate mixed in a mass ratio of 2:0.5:1:1) 1 part by weight; Add deionized water to a total of 100 parts by weight; Preparation method: Step 1: Preparation of the premixed solution of active ingredients Add betaine to 1 / 3 of the formula volume of deionized water and stir until completely dissolved to obtain a betaine solution; add heme to a small amount of 10% ammonia water and stir until completely dissolved to obtain a heme solution; add S-inducer to a small amount of ethanol and stir until completely dissolved, then add it to the spermine solution, then add the heme solution, and stir at room temperature until all components are completely dissolved and mixed evenly to obtain the active ingredient premixed solution; Step 2: Preparation of the premixed solution of the synergistic ingredients Take 1 / 3 of the total mass of the formula and heat it to 30~35℃. Add chitosan oligosaccharide and stir until completely dissolved. Then cool to room temperature. After cooling, add 5-aminolevulinic acid and continue stirring until completely dissolved to obtain the premixed solution of the synergistic ingredients. Step 3: Preparation of premixed solution of auxiliary agent components Take the remaining 1 / 3 of the total mass of deionized water, add Tween-80 and sodium dodecylbenzenesulfonate, stir at 150-200 r / min until emulsified and uniform, then add the trace element composition, and continue stirring until completely dissolved to obtain the premixed solution of the auxiliary components; Step 4: Preparation of the composite regulator While stirring, slowly add the premixed solution of the active ingredient to the premixed solution of the synergistic ingredient, controlling the addition time at 5-8 minutes. After the addition is complete, continue stirring for 10-15 minutes to form a homogeneous and transparent mixture. Subsequently, continue stirring while slowly adding the premixed solution of the adjuvant ingredient to the homogeneous and transparent mixture, controlling the addition time at 3-5 minutes. After the addition is complete, continue stirring for 15-20 minutes. Adjust the pH to 5.8-6.5 using citric acid solution (10% concentration) or dilute sodium hydroxide solution (0.1 mol / L). Filter, sterilize, dispense into light-proof containers, seal and store to obtain the compound regulator.

[0034] Comparative Example 4 Active ingredients: 0.002 parts by weight of 28-homobrassinolide, 0.03 parts by weight of spermine, and 0.002 parts by weight of melatonin; Synergistic ingredients: 3 parts by weight of chitosan oligosaccharide, 5 parts by weight of purslane polysaccharide, 0.4 parts by weight of salicylic acid, 0.008 parts by weight of hydroxytyrosol, 0.2 parts by weight of isorhamnetin, 1 part by weight of sodium hyaluronate, and 0.006 parts by weight of 5-aminolevulinic acid; Additive components: surfactant (Tween 80) 2 parts by weight, dispersant (sodium dodecyl sulfonate) 0.5 parts by weight, trace element composition (ferric citrate, zinc sulfate, boric acid, and ammonium molybdate mixed in a mass ratio of 2:0.5:1:1) 2 parts by weight; Add deionized water to a total of 100 parts by weight; Preparation method: Step 1: Preparation of the premixed solution of active ingredients Melatonin was added to 1 / 3 of the formula volume of deionized water and stirred in a 45°C water bath until completely dissolved. The solution was then cooled to 30°C to obtain a melatonin solution. Heme chloride was added to a small amount of 10% ammonia water and stirred until completely dissolved to obtain a heme chloride solution. Rhamnolipid was added to a small amount of ethanol and stirred until completely dissolved. This solution was then added to the melatonin solution, followed by the heme chloride solution. The mixture was stirred at room temperature until all components were completely dissolved and homogeneous to obtain a premixed solution of the active ingredients. Step 2: Preparation of the premixed solution of the synergistic ingredients Add a small amount of ethanol to isorhamnetin and stir until completely dissolved to obtain an isorhamnetin ethanol solution; take 1 / 3 of the total mass of deionized water and heat to 30-35℃, add chitosan oligosaccharide, purslane polysaccharide, sodium hyaluronate and salicylic acid, stir until completely dissolved, and then cool to room temperature; after cooling, add hydroxytyrosol and 5-aminolevulinic acid in sequence, continue stirring until completely dissolved, add isorhamnetin ethanol solution, continue stirring until completely dissolved and mixed evenly to obtain a premixed solution of synergistic ingredients; Step 3: Preparation of premixed solution of auxiliary agent components Take the remaining 1 / 3 of the total mass of deionized water, add Tween-80 and sodium dodecylbenzenesulfonate, stir at 150-200 r / min until emulsified and uniform, then add the trace element composition, and continue stirring until completely dissolved to obtain the premixed solution of the auxiliary components; Step 4: Preparation of the composite regulator While stirring, slowly add the premixed solution of the active ingredient to the premixed solution of the synergistic ingredient, controlling the addition time at 5-8 minutes. After the addition is complete, continue stirring for 10-15 minutes to form a homogeneous and transparent mixture. Subsequently, continue stirring while slowly adding the premixed solution of the adjuvant ingredient to the homogeneous and transparent mixture, controlling the addition time at 3-5 minutes. After the addition is complete, continue stirring for 15-20 minutes. Adjust the pH to 5.8-6.5 using citric acid solution (10% concentration) or dilute sodium hydroxide solution (0.1 mol / L). Filter, sterilize, dispense into light-proof containers, seal and store to obtain the compound regulator.

[0035] Comparative Example 5 Active ingredients: rhamnolipid 0.015 parts by weight, choline chloride 0.010 parts by weight, spermine 0.05 parts by weight; Synergistic ingredients: 3 parts by weight of chitosan oligosaccharide, 4 parts by weight of purslane polysaccharide, 0.3 parts by weight of salicylic acid, 0.008 parts by weight of hydroxytyrosol, 0.1 parts by weight of isorhamnetin, 1.2 parts by weight of sodium hyaluronate, and 0.007 parts by weight of 5-aminolevulinic acid; Additive components: surfactant (Tween 80) 1.5 parts by weight, dispersant (sodium dodecyl sulfonate) 1 part by weight, trace element composition (ferric citrate, zinc sulfate, boric acid, and ammonium molybdate mixed in a mass ratio of 2:0.5:1:1) 2 parts by weight; Add deionized water to a total of 100 parts by weight; Preparation method: Step 1: Preparation of the premixed solution of active ingredients Spermine and choline chloride were added to 1 / 3 of the formula volume of deionized water and stirred until completely dissolved to obtain a mixed solution. Heme was added to a small amount of 10% ammonia water and stirred until completely dissolved. Then it was added to the above mixed solution and stirred at room temperature until all components were completely dissolved and mixed evenly to obtain the active ingredient premixed solution. Step 2: Preparation of the premixed solution of the synergistic ingredients Add a small amount of ethanol to isorhamnetin and stir until completely dissolved to obtain an isorhamnetin ethanol solution; take 1 / 3 of the total mass of deionized water and heat to 30-35℃, add chitosan oligosaccharide, purslane polysaccharide, sodium hyaluronate and salicylic acid, stir until completely dissolved, and then cool to room temperature; after cooling, add hydroxytyrosol and 5-aminolevulinic acid in sequence, continue stirring until completely dissolved, add isorhamnetin ethanol solution, continue stirring until completely dissolved and mixed evenly to obtain a premixed solution of synergistic ingredients; Step 3: Preparation of premixed solution of auxiliary agent components Take the remaining 1 / 3 of the total mass of deionized water, add Tween-80 and sodium dodecylbenzenesulfonate, stir at 150-200 r / min until emulsified and uniform, then add the trace element composition, and continue stirring until completely dissolved to obtain the premixed solution of the auxiliary components; Step 4: Preparation of the composite regulator While stirring, slowly add the premixed solution of the active ingredient to the premixed solution of the synergistic ingredient, controlling the addition time at 5-8 minutes. After the addition is complete, continue stirring for 10-15 minutes to form a homogeneous and transparent mixture. Subsequently, continue stirring while slowly adding the premixed solution of the adjuvant ingredient to the homogeneous and transparent mixture, controlling the addition time at 3-5 minutes. After the addition is complete, continue stirring for 15-20 minutes. Adjust the pH to 5.8-6.5 using citric acid solution (10% concentration) or dilute sodium hydroxide solution (0.1 mol / L). Filter, sterilize, dispense into light-proof containers, seal and store to obtain the compound regulator.

[0036] Effect Verification: The Influence of Different Compound Regulators on Photosynthesis in Crop Seedlings under Stress 1. Materials and Methods High-temperature stress: The test material consisted of strawberry plug seedlings with similar growth. After 7 days of acclimatization, the seedlings were treated with different spraying methods: the control group and the blank group were sprayed with water, while experimental groups 1-3 were sprayed with different compound growth regulators (diluted 500 times). Forty seedlings were treated with each method. On the third day after spraying, all groups except the blank group underwent high-temperature treatment: the seedlings were moved into a light incubator with a light intensity of 2000 lx and a temperature of 38℃. Samples were taken at 0h, 1h, 3h, 6h, and 10h of stress to measure photosynthetic parameters.

[0037] Drought stress: The test material consisted of maize seedlings (three-leaf stage) of similar growth, planted in plastic pots. Different spraying treatments were applied to the seedlings, once every other day for a total of two applications. The control group, control group 1, and control group 2 were sprayed with water, while experimental groups 1-4 were sprayed with different compound growth regulators (diluted 300 times). Four days after the last spraying, all groups except the control group underwent drought treatment. Control group 1, experimental groups 1-1, 1-2, and 1-3 experienced mild drought (soil moisture content 7-9%), while control group 2, experimental groups 2-1, 2-2, and 2-3 experienced severe drought (soil moisture content 3-5%). The control group had a soil moisture content of 13-15%. Ten pots were used for each treatment, with three seedlings per pot. On the fourth day of drought treatment, leaves were collected for photosynthetic index measurement.

[0038] Low-temperature stress: The test material consisted of Chinese cabbage seedlings with similar growth. Different spraying treatments were applied, once every two days for a total of two applications. The control group and the blank group were sprayed with water, while experimental groups 1-3 were sprayed with different compound growth regulators (diluted 500 times). Twenty seedlings were used for each treatment. Three days after the last spraying, all groups except the blank group underwent low-temperature treatment: the seedlings were placed in a light incubator with a light intensity of 22000 lx, a daytime photoperiod of 12 hours, and a temperature of 5℃. Photosynthetic indicators were measured two days after the low-temperature stress.

[0039] Low-light treatment: The test material consisted of cucumber seedlings (6-7 true leaves) with similar growth. Different spraying treatments were applied to the seedlings, once every 3 days for a total of 2 applications. The control group and the blank group were sprayed with water, while experimental groups 1-3 were sprayed with different compound growth regulators (diluted 500 times). Each treatment involved 30 seedlings. Three days after the last spraying, all groups except the blank group underwent low-light treatment: placed in an AI climate chamber with a light intensity of 500 lx, a photoperiod of 12h / 12h, humidity of 60-70%, and temperature of 25℃ for 5 days. Samples were then taken for photosynthetic index measurement.

[0040] 2. Results and Analysis 2.1 Effects of high temperature on strawberry photosynthesis As shown in Table 1, spraying the regulator can increase the chlorophyll content in the leaves. After high temperature stress, the chlorophyll content shows a decreasing trend. However, the chlorophyll content in the leaves sprayed with the regulator of Example 1 is higher than that in Comparative Example 1 and Comparative Example 4.

[0041] Table 1. Effects of different regulators on chlorophyll content in strawberry under high temperature stress Note: '&' indicates that compared with the blank group, P < 0.05; '&' indicates that compared with the control group, P < 0.05; '#' indicates that compared with experimental group 1, P < 0.05.

[0042] As shown in Table 2, strawberry leaves under high temperature stress exhibited stomatal closure, and stomatal conductance gradually decreased over time. The stomatal conductance of strawberry leaves sprayed with the regulator also showed a decreasing trend, but the overall situation was better than that of the control group. Among them, the stomatal conductance of leaves sprayed with the regulator of Example 1 was higher, and the decreasing trend was relatively stable. It has a better regulatory effect on gas exchange in photosynthesis of leaves under high temperature conditions, and the effect is significantly better than that of leaves sprayed with the regulators of Comparative Example 1 and Comparative Example 4.

[0043] Table 2 Effects of different regulators on stomatal conductance of strawberries under high temperature stress Note: '&' indicates that compared with the blank group, P < 0.05; '&' indicates that compared with the control group, P < 0.05; '#' indicates that compared with experimental group 1, P < 0.05.

[0044] 2.2 The impact of drought on maize photosynthesis As shown in Table 3, under drought stress, the photosynthetic rate, chlorophyll content, and photosynthetic enzyme activity of maize leaves all showed a downward trend, and MDA in the leaves continued to accumulate and its content showed an upward trend; however, the condition of maize leaves sprayed with regulators was improved, and the photosynthetic effect of leaves sprayed with regulators from Examples 2 and 3 was higher than that of the comparative example.

[0045] Table 3 Effects of different regulators on maize photosynthesis under drought stress Note: 'P' indicates that compared with the blank group, P < 0.05; '&' indicates that compared with control group 1-1 or control group 2-1, P < 0.05; '#' indicates that compared with test group 1-1 or test group 2-1, P < 0.05.

[0046] 2.3 Effects of Low Temperature on Photosynthesis in Chinese Cabbage As shown in Table 4, the photosynthetic rate and stomatal conductance of Chinese cabbage decreased significantly under low-temperature stress. The photosynthetic rate and stomatal conductance of the Chinese cabbage leaves sprayed with the regulator also showed a decreasing trend. However, the photosynthetic rate and stomatal conductance of the Chinese cabbage sprayed with the regulator of Example 4 were basically no different from the control group, and were higher than those of the Chinese cabbage leaves sprayed with the regulators of Comparative Examples 3 and 5. Comparison of the fresh weight of the Chinese cabbage revealed that the decrease in photosynthesis under low-temperature conditions slowed the growth of Chinese cabbage, but the growth of the Chinese cabbage in Experiment 1 was normal.

[0047] Table 4. Effects of different regulators on photosynthesis in Chinese cabbage under low temperature stress Note: '&' indicates that compared with the blank group, P < 0.05; '&' indicates that compared with the control group, P < 0.05; '#' indicates that compared with experimental group 1, P < 0.05.

[0048] 2.4 Effects of weak light on cucumber photosynthesis As shown in Table 5, under low light conditions, the photosynthesis of pepper seedlings decreased, but the photosynthesis improved after spraying the regulator. Among them, the photosynthesis of the control group was basically the same after spraying Example 5, while the effect of the regulator in Comparative Examples 3 and 5 was relatively poor.

[0049] Table 5. Effects of different regulators on photosynthesis in cucumbers under low light treatment. Note: '&' indicates that compared with the blank group, P < 0.05; '&' indicates that compared with the control group, P < 0.05; '#' indicates that compared with experimental group 1, P < 0.05.

Claims

1. A composite regulator, characterized in that, It contains the following ingredients: Active ingredients, synergistic ingredients, adjuvant ingredients, water; The active ingredient comprises: 3-4 parts by weight of heme or heme chloride, 0.0005-0.002 parts by weight of brassinolide, and 0.01-0.07 parts by weight of spermine; wherein the brassinolide is 28-homobrassinolide or 14-hydroxysterol brassinolide. The synergistic components include: 2-3 parts by weight of chitosan oligosaccharide, 3-6 parts by weight of purslane polysaccharide, 0.2-0.4 parts by weight of salicylic acid, 0.008-0.010 parts by weight of hydroxytyrosol, 0.1-0.2 parts by weight of isorhamnetin, 1-1.5 parts by weight of sodium hyaluronate, and 0.006-0.008 parts by weight of 5-aminolevulinic acid; The auxiliary agent contains: 1-2 parts by weight of surfactant, 0.5-1 parts by weight of dispersant, and 1-2 parts by weight of trace element composition; The trace element composition contains ferric citrate, zinc sulfate, boric acid, and ammonium molybdate.

2. The composite regulator as described in claim 1, characterized in that, The active ingredients contain: 3-3.5 parts by weight of heme or heme chloride, 0.001-0.002 parts by weight of 28-homobrassinolide, and 0.01-0.03 parts by weight of spermine.

3. The composite regulator as described in claim 1, characterized in that, The active ingredients contain: 3-4 parts by weight of heme or heme chloride, 0.0005-0.0010 parts by weight of 14-hydroxysterol brassinolide, and 0.05-0.07 parts by weight of spermine.

4. The composite regulator according to any one of claims 1 to 3, characterized in that, The mass ratio of ferric citrate, zinc sulfate, boric acid, and ammonium molybdate in the trace element composition is 2:0.5:1:

1.

5. A method for preparing the composite regulator as described in any one of claims 1 to 4, characterized in that, Includes the following steps: Step 1: Preparation of the premixed solution of active ingredients The active ingredients are dissolved separately in water, ethanol, or ammonia, and then mixed to obtain a premixed solution of the active ingredients. Step 2: Preparation of the premixed solution of the synergistic ingredients Dissolve the synergistic ingredients separately in ethanol or water, mix them, and obtain a premixed solution of the synergistic ingredients; Step 3: Preparation of premixed solution of auxiliary agent components Dissolve the auxiliary components in water to obtain a premixed solution of the auxiliary components; Step 4: Preparation of the composite regulator While stirring, the premixed solution of active ingredients is slowly added to the premixed solution of synergistic ingredients, and stirred to form a homogeneous and transparent mixture. Then, while stirring, the premixed solution of adjuvant ingredients is slowly added to the homogeneous and transparent mixture, and stirring is continued. The pH is adjusted to 5.8-6.5, filtered, sterilized, dispensed into light-proof containers and sealed for storage to obtain the compound regulator.

6. The application of a composite regulator as described in any one of claims 1 to 4 in improving crop photosynthesis.

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