A seaweed prebiotic composition for fortifying the immune health of cats, and methods of making and using the same

Abstract

This invention discloses a seaweed prebiotic composition for enhancing feline immune health, its preparation method, and its application. The composition comprises the following raw materials: alginate oligosaccharide, seaweed complex extract, plant-derived prebiotics, a food-grade carrier, and an anti-caking agent. With alginate oligosaccharide as the core functional component, it is compounded with natural seaweed complex extract and plant-derived complex prebiotics, supplemented by a food-grade carrier and an anti-caking agent for precise formulation. This overcomes the limitations of traditional single alginate oligosaccharide formulations, which have a single target and limited efficacy. The components work synergistically and complement each other: alginate oligosaccharide primarily exerts antioxidant, anti-inflammatory, and prebiotic effects; the seaweed complex extract supplements seaweed polysaccharides, alginate polyphenols, and natural mineral active substances, helping to enhance the body's immune reserves; and the plant-derived prebiotics provide a dedicated carbon source for beneficial intestinal bacteria, further amplifying the intestinal microecological regulation effect. Overall, this achieves a synergistic effect of antioxidant, anti-inflammatory, and intestinal microecological regulation.

Description

Technical Field

[0001] This invention relates to the field of pet care technology, specifically to an algae prebiotic composition for enhancing the immune health of cats, its preparation method, and its application. Background Technology

[0002] With the continuous increase in pet ownership, cats have become the mainstream companion animal breed, and the number of cats kept is rising year by year. The health care needs of cats are gradually shifting from basic sustenance to more refined functional care, including immune system enhancement, intestinal regulation, stress reduction, and anti-inflammatory and antioxidant effects. Clinical and breeding practices have revealed that domesticated cats currently commonly suffer from sub-health issues such as weak immunity, susceptibility to seasonal stress, recurrent skin inflammation, sensitive and fragile intestines, soft or loose stools, poor digestion and absorption, and a high incidence of subclinical low-grade inflammation. These issues seriously affect the cats' lifespan, coat condition, and quality of life.

[0003] The gut is the largest immune organ in cats, and the homeostasis of the gut microbiota directly determines the body's immune level, nutrient absorption capacity, and inflammation regulation ability. Microbiota imbalance, insufficient abundance of beneficial bacteria, and excessive proliferation of harmful bacteria are core causes of diarrhea, soft stools, allergic dermatitis, worsened tear stains, and decreased immunity in cats. Currently, commonly used regulatory methods in the industry mostly involve single-supplementation of probiotics, ordinary prebiotics, vitamin antioxidants, or herbal extracts. These methods have shortcomings such as single target, poor synergy, slow onset of action, insufficient targeting, susceptibility to gastric acid, and weak colonization ability, making it difficult to simultaneously achieve the triple effects of anti-oxidation, anti-inflammation, and microbiota regulation.

[0004] Alginic acid oligosaccharides (AOS) are small-molecule functional oligosaccharides prepared from marine alginate through enzymatic hydrolysis or physical degradation. They are characterized by their small molecular weight, high water solubility, non-antigenicity, and safety. AOS has been proven to possess multiple physiological activities, including antioxidant activity, free radical scavenging, anti-inflammatory and antibacterial effects, immune regulation, intestinal mucosal repair, and prebiotic proliferation of beneficial bacteria. Current research primarily focuses on applications in livestock and canine farming, demonstrating that AOS can significantly improve intestinal health and antioxidant capacity in animals. However, there is a serious lack of specialized formulations, appropriate dosages, compounding systems, and industrial-scale preparation processes tailored to the specific physiological and metabolic characteristics, intestinal flora structure, and immune regulatory pathways of cats. Furthermore, there is a lack of standardized seaweed prebiotic compositions specifically formulated for cat food.

[0005] Currently, there are still many technical shortcomings in the market for functional pet additives: First, most are single active ingredients without scientific compounding, resulting in limited functionality and improvement effects, failing to simultaneously address antioxidant, anti-inflammatory, and intestinal microecological regulation; Second, common prebiotic raw materials have poor compatibility and weak targeted proliferation effects on cat intestinal flora, failing to effectively improve flora diversity and stability; Third, some products add hormones, antibiotics, and chemical anti-inflammatory components, which may show short-term effects but pose long-term risks to liver and kidneys, drug resistance, and safety; Fourth, pure seaweed oligosaccharide powder is prone to moisture absorption and clumping, has poor flowability, low mixing uniformity with cat food, and poor storage stability, making it unsuitable for industrial production and long-term storage; Fifth, existing products are not formulated to address the physiological differences of kittens, adult cats, senior cats, and cats with stress and weakness, resulting in a narrow target population and weak targeting.

[0006] Meanwhile, existing experimental studies only focus on basic observations of single AOS effects, without conducting multi-component synergistic formulations, process optimization, dosage form development, quantitative feeding dosage, and multi-dimensional indicator verification, thus failing to develop feasible patented formulations and production application solutions. Based on the current urgent needs for pet cat health care, the technological deficiencies of industry products, and the natural bioactivity advantages of alginate oligosaccharides, this invention specifically develops a cat-specific seaweed prebiotic composition with alginate oligosaccharides as the core, combined with natural seaweed extracts and plant-derived prebiotics. Through scientific formulation and a gentle preparation process, it overcomes the industry pain points of existing technologies, such as single formulations, weak efficacy, poor stability, and insufficient compatibility, providing a new technical solution and industrialization support for the development of functional additives in cat food and cat immune and intestinal health products. Summary of the Invention

[0007] The purpose of this invention is to provide an algae prebiotic composition for enhancing the immune health of cats, its preparation method, and its application, so as to solve the problems mentioned in the background art.

[0008] To achieve the above objectives, the present invention provides the following technical solution: a seaweed prebiotic composition for enhancing the immune health of cats, comprising the following raw materials in parts by weight: 80-95 parts of alginate oligosaccharide, 2-15 parts of seaweed compound extract, 3-8 parts of plant-derived prebiotics, 2-6 parts of food-grade carrier, and 0.1-0.5 parts of anti-caking agent.

[0009] Preferably, the alginate oligosaccharide is a small-molecule water-soluble oligosaccharide obtained by enzymatic hydrolysis of marine alginate, with a molecular weight of 1000-5000 Da, a degree of polymerization of 2-10, and a purity of ≥90%.

[0010] Preferably, the seaweed compound extract is composed of one or more of kelp extract, nori extract, and wakame extract in any proportion, and is rich in seaweed polysaccharides, brown algae polyphenols, mannitol, and natural mineral elements.

[0011] Preferably, the plant-derived prebiotic is any one or more of fructooligosaccharides, galactooligosaccharides, and inulin; the food-grade carrier is one or more of corn starch, maltodextrin, and defatted rice bran powder; and the anti-caking agent is any one of food-grade silicon dioxide and magnesium stearate.

[0012] Preferably, the composition is a preferred limited formulation, consisting of the following preferred raw materials in parts by weight: 90 parts of alginate oligosaccharide, 6 parts of seaweed complex extract, 4 parts of fructooligosaccharide, 3.5 parts of maltodextrin, and 0.3 parts of silicon dioxide.

[0013] A method for preparing an algae prebiotic composition to enhance the immune health of cats includes the following steps:

[0014] S1: Raw material pretreatment: Pass each raw material through an 80-100 mesh standard sieve to remove lumps and impurities and ensure uniform particle size.

[0015] S2: Graded premixing: First, premix the alginate oligosaccharide and anti-caking agent for 5-10 minutes, then add the seaweed complex extract and plant-derived prebiotics and continue mixing, and finally add the food-grade carrier gradient ingredients.

[0016] S3: Constant temperature mixing: Put the premixed material into a three-dimensional motion mixer and stir in a closed environment at 20-30℃ and 25-35r / min for 15-30min.

[0017] S4: Mild sterilization: Sterilize by ultraviolet irradiation for 10-15 minutes or low-temperature pasteurization to retain bioactive components;

[0018] S5: Aseptic packaging: The product is sealed and packaged in a Class 10,000 cleanroom, and stored in a dark and moisture-proof environment to obtain the finished product composition.

[0019] Application of an algae prebiotic composition for enhancing the immune health of cats, wherein the composition is uniformly mixed at an addition ratio of 1g / kg of basic cat food, and each cat is fed 80-100g daily for 28-30 consecutive days.

[0020] Preferably, the composition is fed daily at a dose of 0.04–0.06 g / kg of cat body weight and is suitable for use in feeding kittens, senior cats, cats recovering from illness, and cats that are weak due to stress.

[0021] Preferably, the seaweed prebiotic composition is suitable for daily feeding scenarios of family pet cats, breeding catteries, boarding cat groups, and cats recovering in pet hospitals.

[0022] Preferably, the composition can be compounded with one or more of cat food ingredients, pet nutritional matrix, edible flavoring agents, and food-grade binders to process into any cat preparation form such as powder, paste, tablet, or granule.

[0023] Compared with existing technologies, the beneficial effects of this invention are as follows: Using alginate oligosaccharides as the core functional component, combined with natural seaweed complex extracts and plant-derived complex prebiotics, and supplemented with food-grade carriers and anti-caking agents in a precise formulation, it overcomes the shortcomings of traditional single alginate oligosaccharides, which have limited efficacy and target specificity. The components work synergistically and complement each other: alginate oligosaccharides primarily exert antioxidant, anti-inflammatory, and prebiotic effects; seaweed complex extracts supplement seaweed polysaccharides, alginate polyphenols, and natural mineral active substances, helping to enhance the body's immune reserves; plant-derived prebiotics provide a dedicated carbon source for beneficial intestinal bacteria, further amplifying the intestinal microecological regulation effect. Overall, it achieves a synergistic effect of antioxidant, anti-inflammatory, and intestinal microecological regulation, with overall efficacy far superior to that of using a single ingredient alone.

[0024] This composition can significantly increase the total antioxidant level of T-AOC in feline serum, effectively scavenge free radicals in the body, reduce the content of MDA (malondialdehyde) oxidative damage markers, and alleviate oxidative stress caused by stress, seasonal changes, environmental changes, and diet changes in cats. It can stabilize the body's antioxidant defense system, reduce cellular lipid peroxidation damage, and delay the decline of bodily functions, making it especially suitable for long-term health maintenance in senior cats, stressed cats, and breeding cats.

[0025] The composition significantly downregulates the expression level of the core pro-inflammatory factor TNF-α in cats, while simultaneously causing a sustained and benign decline in inflammation-related indicators such as IL-1β, IL-6, and SAA. It effectively inhibits low-grade intestinal inflammation, skin inflammation, and systemic subclinical inflammation in cats. Unlike the generalized effects of ordinary anti-inflammatory products, this invention has targeted regulation of feline inflammatory pathways, contains no hormones or chemical anti-inflammatory ingredients, and achieves gentle and long-lasting anti-inflammatory effects through natural active ingredients, making it less likely to induce drug resistance or burden the body.

[0026] After feeding the combination, it can significantly improve the Ace flora richness index, Shannon diversity index, and Simpson community balance index of the cat's gut, increase the number of core OTUs and specific ASVs in the gut; effectively promote the abundance of beneficial bacteria such as Actinobacteria and Bacteroidetes, moderately regulate the proportion of bacteria such as Firmicutes, and reshape the gut microbiota structure. At the same time, it can improve the overall variability and stability of the gut microbiota, enhance the gut microbiota's resistance to interference, and fundamentally improve common problems in cats such as intestinal sensitivity, soft stools, indigestion, and poor absorption, thus building a strong first line of defense for intestinal immunity.

[0027] By rationally combining food-grade carriers and trace amounts of anti-caking agents, the powder's flowability, dispersibility, and stacking properties are optimized, effectively addressing the shortcomings of pure alginate oligosaccharides, such as easy moisture absorption, clumping, and uneven dispersion. The product has a long shelf life under cool, dry conditions, is not easily affected by moisture or spoilage, exhibits high uniformity when mixed with cat food ingredients, and is compatible with various pet food production processes, including extruded, baked, air-dried, and freeze-dried foods, without requiring modifications to existing production lines.

[0028] The process employs ambient temperature sieving, gradient premixing, three-dimensional closed mixing, and gentle sterilization, with no high temperature, high pressure, or chemical modification treatment throughout, maximizing the preservation of the bioavailability of heat-sensitive active substances such as alginate oligosaccharides and seaweed polysaccharides. The process is standardized, easy to operate, energy-efficient, uses readily available raw materials, and has controllable production costs. It can be industrially added to cat food in large quantities or produced in small batches as nutritional pastes, powders, chewable tablets, and other end-product health supplements, demonstrating strong industrial applicability.

[0029] The composition can be added directly to commercial cat food at a fixed dosage as a feed additive, or it can be further processed into various dosage forms such as oral food mixing powder, intestinal conditioning nutritional paste, immune-enhancing chewable tablets, and freeze-dried food mixing companion. It can meet the daily health care needs of family pet cats, and is also suitable for mass immunization and intestinal conditioning of breeding catteries, pet boarding facilities, and postoperative recovery groups in pet hospitals. It has a wide range of commercial applications and high market promotion value. Detailed Implementation

[0030] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0031] Example 1

[0032] A prebiotic composition of seaweed to enhance the immune health of cats is prepared from the following raw materials in parts by weight: 90 parts of alginate oligosaccharide, 6 parts of kelp and laver compound extract, 4 parts of fructooligosaccharide, 3.5 parts of maltodextrin, and 0.3 parts of silicon dioxide.

[0033] Preparation method:

[0034] S1: Raw material pretreatment: Pass each raw material through a 100-mesh standard sieve to remove lumps and impurities and ensure uniform particle size.

[0035] S2: Graded premixing: First, premix the alginate oligosaccharide and anti-caking agent for 8 minutes, then add the seaweed complex extract and plant-derived prebiotics and continue to mix. Finally, add the food-grade carrier gradient ingredients.

[0036] S3: Constant temperature mixing: Put the premixed materials into a three-dimensional motion mixer and stir in a closed environment at 25℃ and 30r / min for 20min.

[0037] S4: Mild sterilization: Sterilizes with ultraviolet irradiation for 12 minutes to retain bioactive components;

[0038] S5: Aseptic packaging: The product is sealed and packaged in a Class 10,000 cleanroom, and stored in a dark and moisture-proof environment to obtain the finished product composition.

[0039] Example 2

[0040] A prebiotic composition of seaweed for enhancing the immune health of cats is prepared from the following raw materials in parts by weight: 85 parts alginate oligosaccharide, 10 parts wakame extract, 5 parts inulin, 4 parts corn starch, and 0.2 parts magnesium stearate.

[0041] Preparation method:

[0042] S1: Raw material pretreatment: Pass each raw material through a 90-mesh standard sieve to remove lumps and impurities and ensure uniform particle size.

[0043] S2: Graded premixing: First, premix the alginate oligosaccharide and anticaking agent for 6 minutes, then add the seaweed complex extract and plant-derived prebiotics and continue to mix. Finally, add the food-grade carrier gradient ingredients.

[0044] S3: Constant temperature mixing: Put the premixed materials into a three-dimensional motion mixer and stir in a closed environment at 28℃ and 38r / min for 25min.

[0045] S4: Mild sterilization: pasteurizes at 60℃ to retain bioactive components;

[0046] S5: Aseptic packaging: The product is sealed and packaged in a Class 10,000 cleanroom, and stored in a dark and moisture-proof environment to obtain the finished product composition.

[0047] Example 3

[0048] A seaweed prebiotic composition for enhancing the immune health of cats is prepared from the following raw materials in parts by weight: 92 parts alginate oligosaccharide, 3 parts seaweed complex extract, 3 parts galactooligosaccharide, 2.5 parts defatted rice bran powder, and 0.4 parts silica.

[0049] Preparation method:

[0050] S1: Raw material pretreatment: Pass each raw material through a 100-mesh standard sieve to remove lumps and impurities and ensure uniform particle size.

[0051] S2: Graded premixing: First, premix the alginate oligosaccharide and anti-caking agent for 8 minutes, then add the seaweed complex extract and plant-derived prebiotics and continue to mix. Finally, add the food-grade carrier gradient ingredients.

[0052] S3: Constant temperature mixing: Put the premixed materials into a three-dimensional motion mixer and stir in a closed environment at 30℃ and 32r / min for 18min.

[0053] S4: Mild sterilization: Sterilizes with ultraviolet irradiation for 10 minutes to retain bioactive components;

[0054] S5: Aseptic packaging: The product is sealed and packaged in a Class 10,000 cleanroom, and stored in a dark and moisture-proof environment to obtain the finished product composition.

[0055] Comparative Example 1

[0056] Without adding any seaweed prebiotics or functional additives, the cats were fed only the same basic cat food formula. The breed, age, weight, living environment, feeding amount, and feeding period of the experimental cats were completely consistent with those in Example 1, except that the composition of this invention was not added. Food intake, weight, and fecal scores were recorded synchronously throughout the experiment. Serum T-AOC, MDA, IL-1β, TNF-α, IL-6, SAA, and intestinal flora diversity indicators were measured before and after the experiment as a baseline blank control.

[0057] Comparative Example 2

[0058] Only pure alginate oligosaccharides were added; no seaweed complex extracts, plant-derived prebiotics, carriers, or anti-caking agents were added. The dosage was also 1g / kg of cat food, and the feeding conditions, experimental period, and testing items were exactly the same as in Example 1. Formula: 100 parts pure alginate oligosaccharides, without other compound components. Preparation method: Simple mixing via sieving only; no graded premixing or sterilization process was performed, simulating commercially available single oligosaccharide additives.

[0059] Experimental group design

[0060] Experimental animals: 24 healthy adult female domestic cats aged 1-2 years, weighing 3.2-3.5 kg, were randomly divided into 3 groups of 8 cats each. Husbandry conditions: single cage housing, ambient temperature 22-26℃, humidity 50%-60%, daily feed of 100g / cat, free access to water, experimental period of 30 days.

[0061] Group settings:

[0062] Comparison Example 1: Feeding cats basic food without any functional additives;

[0063] Comparative Example 2: Basic cat food with added pure alginate oligosaccharides 1g / kg;

[0064] Example 1: Add 1g / kg of the seaweed prebiotic composition of this invention to basic cat food.

[0065] Example 2: Add 1g / kg of the seaweed prebiotic composition of this invention to basic cat food.

[0066] Example 3: Add 1g / kg of the seaweed prebiotic composition of this invention to basic cat food.

[0067] Detection indicators: Antioxidant indicators (T-AOC, MDA), as shown in Table 1 below; inflammatory factors (TNF-α, IL-1β, IL-6, SAA), as shown in Table 2 below; gut microbiota alpha diversity, growth and fecal indicators, as shown in Table 3 below.

[0068] Comparative Example 1 0.979±0.105a 3.182±1.623a Comparative Example 2 0.987±0.068ab 2.156±1.215ab Example 1 0.995±0.003c 1.591±0.719c Example 2 0.992±0.011bc 1.725±0.836bc Example 3 0.994±0.006c 1.638±0.752c

[0069] Table 1

[0070] Comparative Example 1 35.46±14.14a 22.14±14.58a 78.62±101.41a 0.140±0.037a Comparative Example 2 20.58±9.65ab 15.36±11.25ab 52.35±68.54ab 0.118±0.029ab Example 1 11.83±4.95b 11.54±9.34b 35.29±45.41b 0.101±0.021b Example 2 13.65±6.28bc 12.86±10.12bc 39.74±51.26bc 0.106±0.024bc Example 3 12.47±5.36c 11.93±9.67c 36.81±47.35c 0.103±0.022c

[0071] Table 2

[0072] Comparative Example 1 228.77±15.14a 3.16±0.26a 0.096±0.029a Comparative Example 2 250.35±22.45ab 3.42±0.31ab 0.075±0.021ab Example 1 271.58±30.36c 3.65±0.28c 0.058±0.015c Example 2 263.42±27.63bc 3.54±0.30bc 0.064±0.018bc Example 3 268.75±29.14c 3.61±0.29c 0.060±0.016c

[0073] Table 3

[0074] Based on the comprehensive analysis of the detection data of antioxidant indicators in Table 1, serum inflammatory factor indicators in Table 2, and gut microbiota Alpha diversity index in Table 3 of this invention, it can be concluded that the three seaweed prebiotic composition formulations with different ratios provided in Examples 1, 2, and 3 of this invention can all effectively act on the physiological metabolism and gut microbiota of cats. They can significantly improve the total antioxidant capacity of cat serum, reduce the level of malondialdehyde oxidative stress damage, and significantly downregulate the expression levels of various inflammatory factors such as tumor necrosis factor, interleukin-1β, interleukin-6, and serum amyloid A. They can also significantly increase the Ace richness index, Shannon diversity index, and Simpson community balance index of cat gut microbiota, comprehensively improve the structure of gut microbiota, and enhance microbiota homeostasis and colonization ability.

[0075] Comparing the blank control group (Comparative Example 1) and Comparative Example 2 (Comparative Example 2 with only alginate oligosaccharide), it can be seen that the three embodiments of the present invention are significantly better than the two comparative examples in terms of antioxidant performance, anti-inflammatory regulation effect, and intestinal flora optimization effect. This fully demonstrates that the design concept of the present invention, which uses alginate oligosaccharide, seaweed compound extract, and plant-derived prebiotics in a multi-component scientific compounding, is reasonable and feasible. The functional components can complement each other and have synergistic effects, producing a significant synergistic effect that is far superior to the effect of using a single raw material alone.

[0076] In a horizontal comparison within the three embodiments, the formulations of Embodiment 1 and Embodiment 3 showed the best overall regulatory effect, with the most significant improvements in antioxidant, anti-inflammatory and gut microbiota effects. The overall efficacy of Embodiment 2 was slightly lower than that of Embodiment 1 and Embodiment 3, but it was still significantly better than the blank control group and the single-ingredient control group. It could also meet the practical application needs of cats for daily immune enhancement, antioxidant stress and gut health conditioning, and the formulation had good compatibility.

[0077] Furthermore, based on the test data related to growth performance and fecal scores, it can be seen that, under normal feeding dosage conditions, all the formulations of this invention did not have a significant adverse effect on the average weight gain, average daily food intake, and fecal formation score of cats. No adverse reactions such as anorexia, vomiting, soft stools, diarrhea, or allergic stress occurred during the feeding process. The product has excellent palatability, high physiological safety, and strong compatibility. It can be used for long-term feeding of kittens, adult cats, senior cats, and cats recovering from illness at all ages. It is fully suitable for industrial production and large-scale market promotion and application as a functional additive for cat food and a raw material for cat immune and intestinal health products.

[0078] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A seaweed prebiotic composition for enhancing the immune health of cats, characterized in that: It is prepared from the following raw materials in parts by weight: 80-95 parts of alginate oligosaccharide, 2-15 parts of seaweed complex extract, 3-8 parts of plant-derived prebiotics, 2-6 parts of food-grade carrier, and 0.1-0.5 parts of anti-caking agent.

2. The seaweed prebiotic composition for enhancing feline immune health according to claim 1, characterized in that: The alginate oligosaccharide is a small-molecule water-soluble oligosaccharide obtained by bio-enzymatic hydrolysis of marine alginate, with a molecular weight of 1000-5000 Da, a degree of polymerization of 2-10, and a purity of ≥90%.

3. The seaweed prebiotic composition for enhancing feline immune health according to claim 2, characterized in that: The seaweed compound extract is composed of one or more of kelp extract, nori extract, and wakame extract in any proportion, and is rich in seaweed polysaccharides, brown algae polyphenols, mannitol, and natural mineral elements.

4. The seaweed prebiotic composition for enhancing feline immune health according to claim 3, characterized in that: The plant-derived prebiotic is any one or more of fructooligosaccharides, galactooligosaccharides, and inulin; the food-grade carrier is one or more of corn starch, maltodextrin, and defatted rice bran powder; and the anti-caking agent is any one of food-grade silicon dioxide and magnesium stearate.

5. The seaweed prebiotic composition for enhancing feline immune health according to claim 4, characterized in that: The composition is a preferred formulation, consisting of the following preferred raw materials in parts by weight: 90 parts alginate oligosaccharide, 6 parts seaweed complex extract, 4 parts fructooligosaccharide, 3.5 parts maltodextrin, and 0.3 parts silicon dioxide.

6. A method for preparing the seaweed prebiotic composition for enhancing feline immune health according to any one of claims 1-5, characterized in that: Includes the following steps: S1: Raw material pretreatment: Pass each raw material through an 80-100 mesh standard sieve to remove lumps and impurities and ensure uniform particle size. S2: Graded premixing: First, premix the alginate oligosaccharide and anti-caking agent for 5-10 minutes, then add the seaweed complex extract and plant-derived prebiotics and continue mixing, and finally add the food-grade carrier gradient ingredients. S3: Constant temperature mixing: Put the premixed material into a three-dimensional motion mixer and stir in a closed environment for 15 to 30 minutes at 20 to 30°C and 25 to 35 r / min. S4: Mild sterilization: Sterilize by ultraviolet irradiation for 10-15 minutes or low-temperature pasteurization to retain bioactive components; S5: Aseptic packaging: The product is sealed and packaged in a Class 10,000 cleanroom, and stored away from light and moisture to obtain the finished product composition.

7. The application of the seaweed prebiotic composition for enhancing feline immune health according to any one of claims 1-5, characterized in that: The composition is evenly mixed at a ratio of 1g / kg of basic cat food. Each cat is fed 80-100g per day for 28-30 consecutive days.

8. The application of the seaweed prebiotic composition for enhancing feline immune health according to claim 7, characterized in that: The composition is fed daily at a rate of 0.04–0.06 g / kg of cat's body weight and is suitable for use in kittens, senior cats, cats recovering from illness, and cats that are weak due to stress.

9. The application of the seaweed prebiotic composition for enhancing feline immune health according to claim 7, characterized in that: The seaweed prebiotic composition is suitable for daily feeding of pet cats in homes, cat breeding territories, boarding cat groups, and cats recovering in veterinary hospitals.

10. The application of the seaweed prebiotic composition for enhancing feline immune health according to claim 7, characterized in that: The composition can be compounded with one or more of cat food ingredients, pet nutritional matrix, edible flavoring agents, and food-grade binders to process into any cat preparation form such as powder, paste, tablet, or granule.