High lutein high zinc and low cholesterol eye-protecting functional chicken egg feed additive, and preparation method and application thereof

Abstract

This invention discloses a high-lutein, high-zinc, low-cholesterol, eye-protecting functional egg feed additive, its preparation method, and its application, belonging to the field of laying hen feed technology. The additive is formulated by compounding marigold lutein microcapsule powder, zinc glycine, mulberry extract, wolfberry polysaccharide, phytosterol esters, yeast β-glucan, complex B vitamins, Bacillus subtilis, modified diatomaceous earth, and resistant dextrin in specific weight parts, and prepared using a low-temperature stabilization process. This invention enhances lutein stability through microencapsulation, improves absorption efficiency through chelated zinc, and synergistically regulates cholesterol metabolism through plant active ingredients. Adding 0.4%~0.6% to the complete feed of laying hens for 12 consecutive days can produce functional eggs with lutein ≥1.2mg / egg, zinc ≥1.0mg / 100g, and cholesterol reduced by 15%~22%. It also improves the stress resistance and egg production rate of laying hens. The product exhibits excellent storage stability and is suitable for large-scale production of eye-protecting functional eggs specifically for teenagers and office workers.

Description

Technical Field

[0001] This invention belongs to the field of processing and application technology of egg-laying hen feed additives, and specifically relates to a high-lutein, high-zinc, low-cholesterol eye-protecting functional egg feed additive, its preparation method, and its application. Background Technology

[0002] In recent years, the myopia rate among Chinese teenagers has continued to rise. Meanwhile, the average daily screen time for working professionals generally exceeds 8 hours, leading to a year-on-year increase in the incidence of eye strain and dry eye syndrome. Consequently, consumer demand for functional foods with eye-protecting properties has exploded. Eggs, as the most widely consumed source of high-quality protein in daily life, contain small amounts of lutein and zinc. However, the natural amounts are insufficient to meet the body's daily eye-protecting needs—a typical egg contains only 0.1-0.2 mg of lutein and approximately 0.5-0.7 mg / 100g of zinc, and the yolk typically contains 1200-1500 mg / 100g of cholesterol. This high cholesterol intake limits consumption for some consumers.

[0003] Existing technologies for producing eye-protecting functional eggs suffer from several drawbacks: First, the efficiency of nutrient fortification is low. Conventional methods directly add ordinary lutein powder and inorganic zinc salts. Lutein is easily deactivated by high temperatures during feed processing, light exposure during storage, and oxidation, resulting in an intestinal absorption rate of less than 30%. Inorganic zinc readily combines with phytic acid and oxalic acid in the hen's intestines to form insoluble complexes, with a deposition efficiency of only about 15%, failing to achieve efficient enrichment of nutrients within the egg. Second, the function is singular. Most existing products focus only on fortifying a single nutrient, without simultaneously designing cholesterol regulation pathways, failing to meet the consumer demand for "high nutrition + low burden." Some products claiming low cholesterol achieve this simply by diluting the yolk, leading to a simultaneous decrease in egg flavor and nutrient density. Third, there are negative impacts on animal health. Some additives, in pursuit of short-term effects, excessively add chemically synthesized components, disrupting the balance of intestinal flora in laying hens, causing problems such as decreased egg production, increased eggshell breakage, and uneven yolk color, resulting in extremely poor stability in livestock applications. Fourth, existing technologies mostly involve simple physical mixing of raw materials without constructing a synergistic effect system between components. For example, when lutein and zinc lack carrier protection, the loss rate can reach more than 40% during feed storage, which cannot meet the stability requirements of large-scale production.

[0004] Existing patents only use ordinary lutein powder and zeaxanthin in combination, which does not solve the problem of lutein oxidation and inactivation. In addition, another existing technology uses zinc sulfate as a zinc source, without considering zinc absorption antagonism and cholesterol regulation. None of the above existing technologies can simultaneously meet the multiple needs of high lutein, high zinc, low cholesterol, and maintenance of laying hen health. Therefore, there is an urgent need to develop a new feed additive with synergistic components, stable process, and verifiable effects. Summary of the Invention

[0005] In view of this, the present invention provides a high-lutein, high-zinc, low-cholesterol eye-protecting functional egg feed additive, its preparation method and application, to solve or alleviate one of the technical problems existing in the prior art, and at least provide a beneficial option.

[0006] The technical solution of this invention is implemented as follows: a high-lutein, high-zinc, low-cholesterol eye-protecting functional egg feed additive and its preparation method and application, which is composed of the following raw materials in parts by weight: 8-12 parts marigold lutein microcapsule powder, 3-5 parts zinc glycine, 4-6 parts mulberry extract, 3-5 parts wolfberry polysaccharide, 5-7 parts phytosterol ester, 4-6 parts yeast β-glucan, 1-2 parts compound B vitamins, 1-2 parts Bacillus subtilis, 25-30 parts modified diatomaceous earth, and 10-15 parts resistant dextrin; The marigold lutein microcapsule powder has an encapsulation rate of ≥90%, the zinc content of the zinc glycinate is 10%, the anthocyanin content of the mulberry extract is ≥25%, and the polysaccharide content of the wolfberry is ≥50%.

[0007] Furthermore, the modified diatomaceous earth is calcined at a high temperature of 200~300℃ and then soaked in a 1% citric acid solution. It has a specific surface area ≥60m² / g and a pore size distribution between 10~50nm, which is used to adsorb volatile and easily oxidized components to improve storage stability.

[0008] Furthermore, the B-complex vitamins are composed of vitamins Vitamins Vitamins It consists of nicotinamide, with a mass ratio of 1:1:0.5:2, and is used to participate in lipid metabolism and nutrient transport in laying hens.

[0009] Furthermore, the viable count of the Bacillus subtilis is ≥2×10¹. 0 CFU / g is used to regulate the intestinal microecological balance of laying hens to promote nutrient absorption.

[0010] Furthermore, this includes the following steps: (1) Stabilization premix: Marigold lutein microcapsule powder, resistant dextrin and modified diatomaceous earth are placed in a mixing device, the ambient temperature is controlled at 30~35℃, and the mixture is mixed for 15min to obtain premix A; (2) Mineral activation: Glycine zinc, yeast β-glucan and complex B vitamins are placed in a mixing device and mixed at room temperature for 10 min to obtain premix B; (3) Plant extract compound: Add premix B to premix A, then add mulberry extract, wolfberry polysaccharide, phytosterol ester and Bacillus subtilis in sequence, mix for 20 min until the components are uniform, and obtain the total mixture; (4) Low-temperature granulation: The total mixture is granulated using granulation equipment, and the air inlet temperature is controlled to be ≤50℃. After obtaining 20~40 mesh granules, they are dried to a moisture content of ≤5%; (5) Screening and packaging: The granulated material is passed through a 20-mesh and a 40-mesh vibrating screen. The material with the middle particle size is vacuum-packed in the dark to obtain the finished product.

[0011] Furthermore, the mixing equipment used in step (1) is a double cone vacuum mixer with a stirring speed of 60 r / min; the mixing equipment used in step (2) is a V-type mixer; and the mixing speed in step (3) is 80 r / min.

[0012] Furthermore, in step (4), a fluidized bed granulator is used for granulation. During the granulation process, the material temperature is controlled to not exceed 40°C, and the drying is carried out using a fluidized bed drying method.

[0013] Furthermore, by adding this additive to the complete feed of laying hens during their egg-laying period at a rate of 0.4% to 0.6% of the feed's weight, and feeding them continuously for 12 days, stable functional eggs with high lutein, high zinc, and low cholesterol can be produced.

[0014] Furthermore, the application can increase the lutein deposition in eggs to ≥1.2mg / egg, zinc content to ≥1.0mg / 100g, reduce yolk cholesterol content by 15%~22% compared to ordinary eggs, increase yolk yellow color fan score to ≥9, and increase egg production rate of laying hens by 2%~4% compared to the basal feed group.

[0015] Furthermore, the application is applicable to Hy-Line Brown, Lohmann Pink, and Jinghong No. 1 commercial laying hens. When fed in a chicken house temperature of 22~25℃ and a relative humidity of 55%~65%, it can achieve stable production of standard eye-protecting functional eggs for more than 42 consecutive days. Moreover, when the additive is stored for 3 months at 40℃ and 75% relative humidity, the lutein retention rate is ≥85%.

[0016] The embodiments of the present invention have the following advantages due to the adoption of the above technical solutions: Significant nutrient enrichment effect: The lutein deposition in the egg is ≥1.2mg / egg, which is more than 6 times higher than that of ordinary eggs; the zinc content is ≥1.0mg / 100g, which is more than 80% higher than that of ordinary eggs. A single egg can meet 15% of the daily lutein and 10% of the zinc intake required for adult eye protection, without the need for additional dietary supplements.

[0017] Cholesterol regulation is clearly defined: the cholesterol content of egg yolks is reduced by 15% to 22% compared to regular eggs. Without changing the original flavor of the eggs, this reduces the cholesterol intake burden on consumers and meets the protein intake needs of people with high blood lipids.

[0018] Egg quality is comprehensively improved: the yolk is uniformly golden yellow, with a Roche color fan score of 9 or above, and no issues such as color layering or spots; the eggshell thickness is increased by 5% to 8%, the breakage rate is reduced by 3% to 5%, and the shelf life is extended by 2 to 3 days.

[0019] Significant benefits to animal health: serum corticosterone levels in laying hens decreased by 18%–25%, their resistance to heat stress and immune stress was improved, egg production increased by 2%–4% compared to the control group, mortality rate decreased by 1.2–1.8 percentage points, and overall profitability for farmers was improved.

[0020] The product exhibits excellent stability: after accelerated storage testing (3 months at 40℃ and 75% relative humidity), the lutein retention rate is ≥85%, the zinc content shows no significant change, and there is no rancid odor, meeting the storage and distribution needs of feed companies.

[0021] The above overview is for illustrative purposes only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features of the invention will become readily apparent from the accompanying drawings and the following detailed description. Attached Figure Description

[0022] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0023] Figure 1 This is a flowchart illustrating the overall process of preparing the feed additive of the present invention. Figure 2 This is a flowchart of the raw material pretreatment process of the present invention. Detailed Implementation

[0024] In the following description, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments can be modified in various ways without departing from the spirit or scope of the invention. Therefore, the drawings and description are considered to be exemplary in nature and not restrictive.

[0025] The embodiments of the present invention will now be described in detail with reference to the accompanying drawings.

[0026] Example 1: Preferred formulation group 1. Raw material preparation Weigh the following raw materials according to the following proportions by weight: 10 parts marigold lutein microcapsule powder (92% encapsulation rate, 20% lutein purity), 4 parts zinc glycine (10.2% zinc content), 5 parts mulberry extract (26.5% anthocyanin content), 4 parts wolfberry polysaccharide (52% polysaccharide content), 6 parts phytosterol esters (95% sterol content), 5 parts yeast β-glucan (70% β-glucan content), and 1.5 parts complex B vitamins (vitamin...). Vitamins Vitamins Nicotinamide (ratio 1:1:0.5:2), 1.5 portions of Bacillus subtilis (2.3 × 10¹ viable cells). 0 CFU / g), 28 parts of modified diatomaceous earth (specific surface area 63m² / g), and 12 parts of resistant dextrin (degree of polymerization 10~15).

[0027] Preparation process (1) Stabilization premix: Marigold lutein microcapsule powder, resistant dextrin, and modified diatomaceous earth were added to a double cone vacuum mixer. The ambient temperature was controlled at 32℃, the stirring speed was 60r / min, and the mixture was mixed for 15min. The RSD of the components was 1.2%, which met the homogeneity requirements. (2) Mineral activation: Zinc glycine, yeast β-glucan, and compound B vitamins were added to a V-type mixer and mixed at room temperature for 10min. The RSD of zinc element dispersion was 0.8%. (3) Plant extract compounding: Premix A was added to the premix. B, add mulberry extract, wolfberry polysaccharide, phytosterol ester and Bacillus subtilis in sequence, stir at 80 r / min, mix for 20 min, and take samples to test the viable bacteria retention rate of 98.7%; (4) Low temperature granulation: use a fluidized bed granulator, with an air inlet temperature of 48℃ and a material temperature of 38℃ to obtain 20~40 mesh granules, and dry them in a fluidized bed to a moisture content of 4.2%; (5) Screening and packaging: pass through 20 mesh and 40 mesh vibrating screens, take the material with the middle particle size, vacuum pack it in aluminum foil bags, and obtain a total of 102 kg of finished product with a yield of 98.1%.

[0028] Feeding experiment 1200 healthy Hy-Line Brown laying hens aged 180 days were randomly divided into two groups, with 6 replicates per group and 100 hens per replicate. The experimental group received a complete feed supplemented with 0.5% of the additive prepared in this embodiment, while the control group was fed a basal complete feed. The pre-trial period was 7 days, and the formal trial period was 28 days. Chickens had free access to feed and water. The temperature in the chicken house was controlled at 22-25℃, and the relative humidity at 55%-65%. During the experiment, the number of eggs laid, egg weight, and number of broken eggs were recorded daily. Egg samples were collected every 7 days to test relevant indicators. The results are as follows: experimental group 93.7±1.2 62.3±0.8 0.7±0.2 2.08±0.03 0.5±0.1 control group 90.2±1.5 61.8±0.7 1.9±0.4 2.17±0.05 2.0±0.3 experimental group 1.31±0.05 1.12±0.03 1085±21 9.2±0.3 control group 0.19±0.02 0.61±0.04 1362±28 6.5±0.4 0 100 100 1085 1362 7 98.2 91.5 1091 1358 14 96.7 83.2 1088 1365 21 94.3 72.6 1092 1360 experimental group 12.3±1.1 3.2±0.2 18.7±0.8 8.9±0.4 control group 16.4±1.3 4.1±0.3 14.2±0.6 7.1±0.3 Example 2: Low-dose formulation group 1. Raw material preparation Weigh the following raw materials according to the following weight proportions: 8 parts marigold lutein microcapsule powder (90% encapsulation rate), 3 parts zinc glycine (10% zinc content), 4 parts mulberry extract (25% anthocyanin content), 3 parts wolfberry polysaccharide (50% polysaccharide content), 5 parts phytosterol esters (95% sterol content), 4 parts yeast β-glucan (70% β-glucan content), 1 part complex B vitamins (same ratio as in Example 1), and 1 part Bacillus subtilis (2 × 10¹ viable bacteria count). 0 CFU / g), 25 parts modified diatomaceous earth, and 10 parts resistant dextrin.

[0029] Preparation process Following the same steps as in Example 1, 78 kg of the finished product was obtained, with a yield of 97.5%.

[0030] Feeding experiment The experimental design was the same as in Example 1, with the experimental group adding 0.4%. The results are as follows: experimental group 92.1±1.3 62.1±0.6 1.0±0.3 2.11±0.04 0.8±0.2 control group 90.0±1.4 61.7±0.8 2.0±0.5 2.18±0.06 2.1±0.4 experimental group 1.23±0.04 1.05±0.02 1127±19 9.0±0.2 control group 0.18±0.01 0.59±0.03 1358±25 6.4±0.3 experimental group 1287±32 1352±29 1421±35 control group 1156±28 1189±31 1213±27 0 88.2±1.1 87.5±1.3 7 86.7±0.9 84.2±1.5 14 84.3±1.2 80.1±1.8 Example 3: High-dose formulation group 1. Raw material preparation Weigh the following raw materials according to the following weight proportions: 12 parts marigold lutein microcapsule powder (encapsulation rate 93%), 5 parts zinc glycine (zinc content 10.3%), 6 parts mulberry extract (anthocyanin content 27%), 5 parts wolfberry polysaccharide (polysaccharide content 53%), 7 parts phytosterol esters (sterol content 96%), 6 parts yeast β-glucan (β-glucan content 71%), 2 parts complex B vitamins (same ratio as in Example 1), and 2 parts Bacillus subtilis (viable count 2.5 × 10¹). 0 CFU / g), 30 parts modified diatomaceous earth, and 15 parts resistant dextrin.

[0031] Preparation process Following the same steps as in Example 1, 125 kg of the finished product was obtained, with a yield of 98.4%.

[0032] Feeding experiment The experimental design was the same as in Example 1, with the experimental group adding 0.6%. The results are as follows: experimental group 94.2±1.1 62.5±0.7 0.6±0.1 2.06±0.02 0.4±0.1 control group 89.8±1.6 61.6±0.9 2.1±0.4 2.19±0.07 2.2±0.5 experimental group 1.35±0.06 1.18±0.04 1062±23 9.3±0.2 control group 0.20±0.02 0.62±0.05 1365±30 6.6±0.4 experimental group 187.2±4.3 256.7±6.1 2.1±0.2 control group 152.4±3.8 208.3±5.2 3.4±0.3 Hyland Brown 1.35±0.05 1.18±0.03 20.8 3.7 Roman powder 1.32±0.04 1.15±0.02 19.7 3.2 Jinghong No. 1 1.33±0.06 1.16±0.04 20.1 3.5 The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any person skilled in the art can easily conceive of various variations or substitutions within the technical scope disclosed in the present invention, and these should all be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A functional egg feed additive with high lutein, high zinc, low cholesterol, and eye protection properties, its preparation method, and its application, characterized in that... It is composed of the following raw materials in parts by weight: 8-12 parts marigold lutein microcapsule powder, 3-5 parts zinc glycine, 4-6 parts mulberry extract, 3-5 parts wolfberry polysaccharide, 5-7 parts phytosterol ester, 4-6 parts yeast β-glucan, 1-2 parts complex B vitamins, 1-2 parts Bacillus subtilis, 25-30 parts modified diatomaceous earth, and 10-15 parts resistant dextrin; The marigold lutein microcapsule powder has an encapsulation rate of ≥90%, the zinc content of the zinc glycinate is 10%, the anthocyanin content of the mulberry extract is ≥25%, and the polysaccharide content of the wolfberry is ≥50%.

2. The functional egg feed additive with high lutein, high zinc, and low cholesterol for eye protection, as described in claim 1, and its preparation method and application, characterized in that... The modified diatomaceous earth is calcined at 200~300℃ and then soaked in a 1% citric acid solution. It has a specific surface area ≥60m² / g and a pore size distribution between 10~50nm. It is used to adsorb volatile and easily oxidized components to improve storage stability.

3. The high-lutein, high-zinc, low-cholesterol eye-protecting functional egg feed additive according to claim 1, its preparation method, and its application, characterized in that, The complex B vitamins are composed of vitamins Vitamins Vitamins It consists of nicotinamide, with a mass ratio of 1:1:0.5:2, and is used to participate in lipid metabolism and nutrient transport in laying hens.

4. The high-lutein, high-zinc, low-cholesterol eye-protecting functional egg feed additive according to claim 1, its preparation method, and its application, characterized in that, The viable cell number of the Bacillus subtilis is ≥2×10¹ 0 CFU / g, for regulating the intestinal microecological balance of laying hens to promote nutrient absorption.

5. The high-lutein, high-zinc, low-cholesterol eye-protecting functional egg feed additive according to claim 1, its preparation method, and its application, characterized in that, Includes the following steps: (1) Stabilization premix: Marigold lutein microcapsule powder, resistant dextrin and modified diatomaceous earth are placed in a mixing device, the ambient temperature is controlled at 30~35℃, and the mixture is mixed for 15min to obtain premix A; (2) Mineral activation: Glycine zinc, yeast β-glucan and complex B vitamins are placed in a mixing device and mixed at room temperature for 10 min to obtain premix B; (3) Plant extract compound: Add premix B to premix A, then add mulberry extract, wolfberry polysaccharide, phytosterol ester and Bacillus subtilis in sequence, mix for 20 min until the components are uniform, and obtain the total mixture; (4) Low-temperature granulation: The total mixture is granulated using granulation equipment, and the air inlet temperature is controlled to be ≤50℃. After obtaining 20~40 mesh granules, they are dried to a moisture content of ≤5%; (5) Screening and packaging: The granulated material is passed through a 20-mesh and a 40-mesh vibrating screen. The material with the middle particle size is vacuum-packed in the dark to obtain the finished product.

6. The high-lutein, high-zinc, low-cholesterol eye-protecting functional egg feed additive according to claim 5, its preparation method, and its application, characterized in that, The mixing equipment used in step (1) is a double cone vacuum mixer with a stirring speed of 60 r / min; the mixing equipment used in step (2) is a V-type mixer; and the mixing speed in step (3) is 80 r / min.

7. The high-lutein, high-zinc, low-cholesterol eye-protecting functional egg feed additive according to claim 5, its preparation method, and its application, characterized in that, In step (4), a fluidized bed granulator is used for granulation. During the granulation process, the material temperature is controlled to not exceed 40°C. The drying process is carried out using a fluidized bed drying method.

8. The high-lutein, high-zinc, low-cholesterol eye-protecting functional egg feed additive according to claim 1, its preparation method, and its application, characterized in that, Adding this additive to the complete feed of laying hens during their egg-laying period at a rate of 0.4% to 0.6% of the feed's weight, and feeding them continuously for 12 days, will result in the production of stable functional eggs with high lutein, high zinc, and low cholesterol.

9. The high-lutein, high-zinc, low-cholesterol eye-protecting functional egg feed additive according to claim 8, its preparation method and application, characterized in that, The application can increase the lutein deposition in eggs to ≥1.2mg / egg, zinc content to ≥1.0mg / 100g, reduce yolk cholesterol content by 15%~22% compared to ordinary eggs, increase yolk yellow color fan score to ≥9, and increase egg production rate of laying hens by 2%~4% compared to the basic feed group.

10. The high-lutein, high-zinc, low-cholesterol eye-protecting functional egg feed additive according to claim 8, its preparation method and application, characterized in that, The application is applicable to Hy-Line Brown, Lohmann Pink, and Jinghong No. 1 commercial laying hens. When fed in a chicken house temperature of 22~25℃ and a relative humidity of 55%~65%, it can achieve stable production of standard eye-protecting functional eggs for more than 42 consecutive days. Furthermore, the additive can be stored for 3 months at 40℃ and 75% relative humidity, with a lutein retention rate of ≥85%.

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