Compositions with soothing and antioxidant efficacy and use thereof, skin external agent

Abstract

This invention discloses a composition with soothing and antioxidant effects, its application, a topical skin preparation, the application of lactoferrin, and the application of sandalwood seed oil. The composition, comprising lactoferrin and sandalwood seed oil, can significantly inhibit the expression of inflammatory factors and significantly activate endogenous antioxidant pathways, effectively improving skin inflammation and oxidative damage.

Description

Technical Field

[0001] This invention relates to the field of cosmetics, and particularly to a composition having soothing and antioxidant effects, its application, and a topical skin preparation. Background Technology

[0002] As the first line of defense between the human body and the external environment, the skin is frequently subjected to multiple forms of damage, including ultraviolet radiation, pollution, allergens, and chemical irritants. These external factors not only directly damage the skin barrier but also induce local inflammatory and oxidative stress responses. Specifically, external stimuli can cause keratinocytes to release large amounts of pro-inflammatory cytokines, leading to discomfort such as redness, stinging, dryness, and itching. Oxidative damage factors, such as ultraviolet radiation, reduce the activity of the skin's endogenous antioxidant enzyme system, causing excessive accumulation of free radicals, further exacerbating the inflammatory response, and creating a vicious cycle. Therefore, developing skincare products with both soothing, anti-inflammatory, and antioxidant effects is of significant practical importance for alleviating sensitive skin problems and repairing the skin barrier.

[0003] Currently, the market offers a wide variety of cosmetic ingredients and formulations with soothing and antioxidant effects. While some ingredients can inhibit inflammatory mediators or scavenge free radicals to some extent, their effects are often singular and their targets are limited, making it difficult to simultaneously and effectively block both inflammatory and oxidative damage pathways. Therefore, there is still room for improvement in overall efficacy. Furthermore, many ingredients with potent antioxidant or anti-inflammatory activities are irritating to the skin. Long-term use or application to sensitive or damaged skin may damage the skin barrier, causing adverse reactions such as stinging and peeling. Summary of the Invention

[0004] To address the aforementioned technical problems, embodiments of the present invention disclose a composition with soothing and antioxidant effects, the composition comprising lactoferrin and sandalwood seed oil.

[0005] Using the above technical solution, lactoferrin and sandalwood seed oil each have anti-inflammatory and antioxidant activities. When combined, they produce a significant synergistic effect, simultaneously enhancing the ability to inhibit inflammatory factors and activate endogenous antioxidant pathways, effectively improving skin inflammation and oxidative damage.

[0006] Optionally, the concentration of lactoferrin is 0.001%-0.005%, and / or the concentration of sandalwood seed oil is 0.001%-0.005%, and / or the total concentration of lactoferrin and sandalwood seed oil is 0.001%-0.005%.

[0007] Optionally, the mass ratio of lactoferrin to sandalwood seed oil is (1-4):(1-4).

[0008] Optionally, the lactoferrin is derived from mammalian milk, and / or the sea buckthorn seed oil is derived from sea buckthorn seeds, and / or the mass ratio of lactoferrin to sea buckthorn seed oil is 1:1.

[0009] Another embodiment of the present invention discloses the use of the above composition in the preparation of an agent that inhibits the expression of inflammatory factors IL-1α (interleukin-1α) and / or IL-8 (interleukin-8).

[0010] Using the above technical solution, the composition can block the expression of IL-1α and IL-8 from the source of inflammation, effectively relieving sensitive symptoms such as skin redness, stinging, dryness and itching.

[0011] Another embodiment of the present invention discloses the use of the above composition in the preparation of an agent that promotes the expression of at least one of the antioxidant-related genes Nrf2 (nuclear factor (erythrocyte-derived 2)-associated factor 2), SOD2 (superoxide dismutase 2), and CAT (catalase).

[0012] Using the above technical solution, the expression of antioxidant genes Nrf2, SOD2 and CAT can be significantly upregulated, thereby enhancing the skin's endogenous antioxidant defense capabilities.

[0013] Another embodiment of the present invention discloses a topical skin agent comprising the composition described above.

[0014] Using the above technical solution, the topical skin agent has both soothing and antioxidant effects, which can effectively relieve discomfort of sensitive skin and improve the skin barrier function.

[0015] Optionally, the topical skin agent is selected from: face cream, lotion, gel, toner, serum, face mask, eye cream, aerosol cleansing foam, shower gel, or facial cleanser.

[0016] Another embodiment of the present invention discloses the use of lactoferrin in the preparation of formulations that inhibit the expression of inflammatory factors IL-1α and / or IL-8.

[0017] Using the above technical solution, lactoferrin can effectively reduce the gene expression of inflammatory factors IL-1α and IL-8, and can be used to prepare anti-inflammatory and soothing agents.

[0018] Another embodiment of the present invention discloses the use of lactoferrin in the preparation of formulations that promote the expression of at least one of the antioxidant-related genes Nrf2, SOD2 and CAT.

[0019] Using the above technical solution, lactoferrin can effectively upregulate the expression of antioxidant-related genes Nrf2, SOD2 and CAT, and can be used to prepare antioxidant preparations.

[0020] Another embodiment of the present invention discloses the use of sandalwood seed oil in the preparation of formulations that inhibit the expression of inflammatory factors IL-1α and / or IL-8.

[0021] Using the above technical solution, sea buckthorn seed oil can effectively reduce the gene expression of inflammatory factors IL-1α and IL-8, and can be used to prepare anti-inflammatory and soothing preparations.

[0022] Another embodiment of the present invention discloses the use of sandalwood seed oil in the preparation of formulations that promote the expression of at least one of the antioxidant-related genes Nrf2, SOD2 and CAT.

[0023] Using the above technical solution, sea buckthorn seed oil can effectively upregulate the expression of antioxidant-related genes Nrf2, SOD2 and CAT, and can be used to prepare antioxidant preparations. Attached Figure Description

[0024] Figure 1 This diagram illustrates the fold increase in IL-1α gene expression inhibition between the experimental and control groups of this invention.

[0025] Figure 2 This diagram illustrates the fold increase in IL-8 gene expression inhibition between the experimental and control groups of the present invention.

[0026] Figure 3 This diagram illustrates the fold increase in Nrf2 gene expression between the experimental and control groups of the present invention.

[0027] Figure 4 This diagram illustrates the fold increase in SOD2 gene expression between the experimental and control groups of the present invention.

[0028] Figure 5 The diagram illustrates the fold increase in CAT gene expression between the experimental and control groups of this invention. Detailed Implementation

[0029] The following specific embodiments illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. Although the description of the present invention will be presented in conjunction with preferred embodiments, this does not mean that the features of the invention are limited to this embodiment. On the contrary, the purpose of describing the invention in conjunction with embodiments is to cover other options or modifications that may be derived based on the claims of the present invention. To provide a deep understanding of the present invention, many specific details will be included in the following description. The present invention may also be implemented without using these details. Furthermore, to avoid confusion or obscuring the focus of the present invention, some specific details will be omitted in the description. It should be noted that, unless otherwise specified, the embodiments and features in the embodiments of the present invention can be combined with each other.

[0030] It should be noted that in this specification, similar reference numerals and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0031] The terms “first”, “second”, etc., are used only to distinguish descriptions and should not be interpreted as indicating or implying relative importance.

[0032] To make the objectives, technical solutions, and advantages of the present invention clearer, the embodiments of the present invention will be described in further detail below with reference to the accompanying drawings.

[0033] The first aspect of the present invention discloses a composition having soothing and antioxidant effects, the composition comprising lactoferrin and sandalwood seed oil.

[0034] In this composition, the inventors, for the first time, combined lactoferrin with sandalwood seed oil, unexpectedly discovering a significant synergistic effect in soothing and anti-oxidation. This combination significantly reduces the expression of inflammatory factors and enhances the expression of transcription factors and enzyme genes related to antioxidant pathways, achieving a soothing and oxidative stress-relieving effect. Both lactoferrin and sandalwood seed oil have the ability to inhibit inflammatory factors and activate endogenous antioxidant pathways. Specifically, the combined use of these two ingredients significantly outperforms the effects of applying the same concentration of lactoferrin or sandalwood seed oil alone, in downregulating IL-1α and IL-8 inflammatory factors and upregulating Nrf2, SOD2, and CAT antioxidant genes. This allows for the simultaneous and efficient blocking of skin inflammation, strengthening the skin's endogenous antioxidant capacity, breaking the vicious cycle of exacerbating skin inflammation and oxidative stress, and achieving soothing and antioxidant skincare effects far exceeding those of single components. Furthermore, the composition is simple and safe, making it suitable for use in soothing skincare creams and lotions for children and adults.

[0035] In the embodiments of the present invention, the concentration of lactoferrin is 0.001%-0.005%. Using this concentration range can ensure that lactoferrin can effectively downregulate IL-1α and IL-8 inflammatory factors and activate endogenous antioxidant pathways, while also ensuring that the raw materials are mild, non-irritating, and non-toxic, and suitable for long-term skin use.

[0036] In an embodiment of the present invention, the concentration of sandalwood seed oil is 0.001%-0.005%. This concentration range can fully exert the anti-inflammatory and antioxidant activities of sandalwood seed oil, fully and stably upregulate the expression of Nrf2, SOD2, and CAT genes, while taking into account the skin feel, making it light and easy to absorb, and suitable for various skin care formulation systems.

[0037] Furthermore, the total concentration of lactoferrin and sandalwood seed oil is 0.001%-0.005%, which can significantly exert soothing and antioxidant effects. At the same time, it is gentle and non-irritating to the skin, making it suitable for further preparation into various skin care products and suitable for use by children and adults.

[0038] In an embodiment of the present invention, the mass ratio of lactoferrin to sandalwood seed oil is (1-4):(1-4), thereby achieving complementary and mutually beneficial effects of the two active ingredients, so that the composition has significantly better effects than the single component in anti-inflammatory and antioxidant pathways, and has strong compatibility.

[0039] Preferably, the mass ratio of lactoferrin to sandalwood seed oil is 1:1. Under this optimal ratio, the efficacy of the two raw materials is balanced and matched, and the inhibitory effect on inflammatory factors and the activation effect on antioxidant genes reach their peak, with the most significant synergistic effect.

[0040] In embodiments of the present invention, lactoferrin is derived from mammalian milk. Specifically, lactoferrin is obtained from mammalian milk through processes such as separation, filtration, and concentration. Preferably, lactoferrin is derived from cow's milk, as cow's milk-derived lactoferrin has high purity, stable activity, and high safety, and is mature in cosmetic applications, suitable for large-scale production and topical skin application.

[0041] In an embodiment of the present invention, sea sandalwood seed oil is derived from sea sandalwood seeds. Specifically, sea sandalwood seed oil is obtained from sea sandalwood seeds through processes such as sun-drying, cold pressing, and filtration. The cold-pressing process retains the active ingredients such as simulinic acid and nervonic acid in sea sandalwood seed oil to the greatest extent, ensuring its excellent anti-inflammatory and endogenous antioxidant activity.

[0042] The second aspect of the present invention discloses the use of the compositions of various embodiments in the preparation of formulations that inhibit the expression of inflammatory factors IL-1α and / or IL-8. The compositions can significantly downregulate the gene expression of key skin inflammatory factors IL-1α and IL-8, block the inflammatory cascade reaction from the source, and effectively improve skin redness, dryness, itching and sensitivity caused by ultraviolet radiation and other stimuli.

[0043] The third aspect of the present invention discloses the use of the compositions of various embodiments in the preparation of formulations that promote the expression of at least one of the antioxidant-related genes Nrf2, SOD2 and CAT. The compositions can effectively activate the core antioxidant pathway of the skin, upregulate the expression of antioxidant genes Nrf2, SOD2 and CAT, enhance the skin's endogenous free radical scavenging ability, and alleviate oxidative stress damage.

[0044] A fourth aspect of the present invention discloses a topical skin agent comprising the composition described in various embodiments, which can stably carry the synergistic active system of lactoferrin and sandalwood seed oil to achieve a mild, long-lasting soothing, anti-inflammatory, and antioxidant repair effect.

[0045] Specifically, topical skin agents are selected from: face creams, lotions, gels, toners, serums, face masks, eye creams, aerosol cleansing foams, shower gels, or facial cleansers.

[0046] Preferably, the topical skin agent is a skin care product.

[0047] In embodiments of the present invention, the topical skin agent further includes, but is not limited to, the following components:

[0048] Emulsifiers: such as glyceryl stearate, cetearyl alcohol, polysorbate, PEG-100 stearate, etc.;

[0049] Thickeners: such as carbomer, xanthan gum, sodium alginate, etc.;

[0050] Moisturizers: such as glycerin, butylene glycol, propylene glycol, betaine, etc.;

[0051] Preservatives: such as phenoxyethanol, methylparaben / propylparaben, potassium sorbate;

[0052] Antioxidants: such as tocopherol (vitamin E), ascorbic acid (vitamin C), disodium EDTA, etc.;

[0053] pH adjusters: such as citric acid, sodium citrate, triethanolamine, etc.

[0054] Skin conditioning agents / active ingredients: such as ceramides, squalane, allantoin, aloe vera extract, asiaticoside, plant extracts (such as green tea, witch hazel, dipotassium glycyrrhizate), etc.;

[0055] Solvents: such as water, ethanol, isopropanol, dipropylene glycol, dioctyl carbonate (used in aerosols);

[0056] Film-forming agents: such as polyvinylpyrrolidone (PVP), polyurethane-35, etc.;

[0057] Surfactants / detergents: such as sodium lauryl ether sulfate, cocamidopropyl betaine, alkyl polyglucoside, etc.

[0058] The fifth aspect of the present invention discloses the use of lactoferrin in the preparation of formulations that inhibit the expression of inflammatory factors IL-1α and / or IL-8. Lactoferrin can significantly inhibit the expression of skin inflammatory factors, has a clear soothing and anti-inflammatory effect, and can be used to improve skin irritation and inflammation.

[0059] The sixth aspect of the present invention discloses the use of lactoferrin in the preparation of formulations that promote the expression of at least one of antioxidant-related genes Nrf2, SOD2 and CAT. Lactoferrin not only has anti-inflammatory capabilities, but can also positively regulate the expression of endogenous antioxidant genes in the skin, and help improve the skin's oxidative state, thus possessing dual efficacy characteristics.

[0060] The seventh aspect of this invention discloses the use of sandalwood seed oil in the preparation of formulations that inhibit the expression of inflammatory factors IL-1α and / or IL-8. Sandalwood seed oil not only possesses antioxidant activity but also directly and significantly inhibits the gene expression of IL-1α and IL-8, thus broadening its application in skincare.

[0061] The eighth aspect of the present invention discloses the use of sandalwood seed oil in the preparation of formulations that promote the expression of at least one of the antioxidant-related genes Nrf2, SOD2 and CAT, providing mechanistic support for the application of sandalwood seed oil in anti-aging and photoprotective products.

[0062] The following will describe the implementation methods in more detail.

[0063] Five sample formulations, group 1-5, were prepared according to the concentration ratios in Table 1.

[0064] Table 1:

[0065]

[0066] Test 1: Lactoferrin, sandalwood seed oil, and their combination inhibited the expression of the inflammatory cytokine IL-1α gene.

[0067] A UVB-damaged keratinocyte assay model was selected. Lactoferrin and sandalwood seed oil were tested for cytotoxicity in human primary keratinocytes, and sample concentrations with activity >80% were screened. The fold increase in IL-1α gene expression was measured using qRT-PCR. The experimental groups consisted of the five samples listed in Table 1. A control group was also included: a blank control (BC) with no treatment, a negative control (NC) with no added sample, and a positive control (PC) with 0.01% dexamethasone. The positive control was only used to verify the model's effectiveness and was not included in the comparison of the composition's effects. Figure 1 This diagram illustrates the fold increase in IL-1α gene expression inhibition between the experimental and control groups.

[0068] Depend on Figure 1 It is evident that, compared with the control group, 0.005% lactoferrin, 0.005% sandalwood seed oil, and the combination of lactoferrin and sandalwood seed oil all significantly reduced the gene expression of the inflammatory factor IL-1α. This indicates that lactoferrin, sandalwood seed oil, and their combination can reduce the expression of IL-1α and achieve a soothing effect.

[0069] Test 2: Lactoferrin, sandalwood seed oil, and their combination inhibited the expression of the inflammatory cytokine IL-8 gene.

[0070] A UVB-damaged keratinocyte assay model was selected. Lactoferrin and sandalwood seed oil were tested for cytotoxicity in human primary keratinocytes, and sample concentrations with activity >80% were screened. The fold increase in IL-8 gene expression was measured using qRT-PCR. The experimental groups consisted of the five samples listed in Table 1. A control group was also included: a blank control (BC) with no treatment, a negative control (NC) with no added sample, and a positive control (PC) with 0.01% dexamethasone. The positive control was only used to verify the model's effectiveness and was not included in the comparison of the composition's effects. Figure 2 This diagram illustrates the fold increase in IL-8 gene expression inhibition between the experimental and control groups.

[0071] Depend on Figure 2 It is evident that, compared with the control group, 0.005% lactoferrin, 0.005% sandalwood seed oil, and the combination of lactoferrin and sandalwood seed oil can all significantly reduce the gene expression of the inflammatory factor IL-8. This indicates that lactoferrin, sandalwood seed oil, and their combination can reduce the expression of IL-8 and achieve a soothing effect.

[0072] Test 3: Lactoferrin, sandalwood seed oil, and their combination promote the expression of the antioxidant-related gene Nrf2.

[0073] A UVB-damaged keratinocyte test model was selected. Lactoferrin and sandalwood seed oil were tested for cytotoxicity in human primary keratinocytes, and sample concentrations with activity >80% were screened. The fold increase in the expression of the antioxidant Nrf2 gene was tested using qRT-PCR. The experimental groups consisted of the five samples listed in Table 1. A control group was also set up, with the untreated model serving as the blank control (BC), the untreated sample as the negative control (NC), and the sample with 0.05% vitamin E as the positive control (PC). The positive control was only used to verify the model's effectiveness and was not included in the comparison of the composition's effects. Figure 3 This diagram illustrates the fold increase in Nrf2 gene expression between the experimental and control groups.

[0074] Depend on Figure 3 It is evident that, compared with the control group, 0.005% lactoferrin, 0.005% sandalwood seed oil, and the combination of lactoferrin and sandalwood seed oil all significantly increased the gene expression level of Nrf2. This indicates that lactoferrin, sandalwood seed oil, and their combination can promote the expression of Nrf2 and achieve an antioxidant effect.

[0075] Test 4: Lactoferrin, sandalwood seed oil, and their combination promote the expression of the antioxidant-related gene SOD2.

[0076] A UVB-damaged keratinocyte test model was selected. Lactoferrin and sandalwood seed oil were tested for cytotoxicity in human primary keratinocytes, and sample concentrations with activity >80% were screened. The fold increase in SOD2 gene expression was tested using qRT-PCR. The experimental groups consisted of the five samples listed in Table 1. A control group was also included: a blank control (BC) with no treatment, a negative control (NC) with no added sample, and a positive control (PC) with 0.05% vitamin E. The positive control was only used to verify the model's effectiveness and was not included in the comparison of the composition's effects. Figure 4 This diagram illustrates the fold increase in SOD2 gene expression between the experimental and control groups.

[0077] Depend on Figure 4 It is evident that, compared with the control group, 0.005% lactoferrin, 0.005% sandalwood seed oil, and the combination of lactoferrin and sandalwood seed oil all significantly increased the gene expression of SOD2. This indicates that lactoferrin, sandalwood seed oil, and their combination can promote the expression of SOD2 and achieve an antioxidant effect.

[0078] Test 5: Lactoferrin, sandalwood seed oil, and their combination promote the expression of the antioxidant-related gene CAT.

[0079] A UVB-damaged keratinocyte assay model was selected. Lactoferrin and sandalwood seed oil were tested for cytotoxicity in human primary keratinocytes, and sample concentrations with activity >80% were screened. The amplification fold of the antioxidant CAT gene expression was tested using qRT-PCR. The experimental groups consisted of the five samples listed in Table 1. A control group was also set up, with the untreated model serving as the blank control (BC), the untreated sample as the negative control (NC), and the sample with 0.05% vitamin E as the positive control (PC). The positive control was only used to verify the model's effectiveness and was not included in the comparison of the composition's effects. Figure 5 This diagram illustrates the fold increase in CAT gene expression between the experimental and control groups.

[0080] Depend on Figure 5 It is evident that, compared with the control group, 0.005% lactoferrin, 0.005% sandalwood seed oil, and the combination of lactoferrin and sandalwood seed oil all significantly increased the gene expression level of CAT. This indicates that lactoferrin, sandalwood seed oil, and their combination can promote CAT expression and achieve an antioxidant effect.

[0081] Furthermore, using the same UVB-damaged human primary keratinocyte model and qRT-PCR detection method as tests 1 to 5, the regulatory effects of the five groups of test samples (Table 1) on the expression levels of two inflammatory factors, IL-1α and IL-8, and three antioxidant genes, Nrf2, SOD2, and CAT, were determined. The statistical results of gene expression in each group are summarized in Table 2 below. In Table 2, groups 1-5 correspond to groups 1-5 in Table 1, respectively.

[0082] Table 2:

[0083]

[0084] Note: "vs lactoferrin -0.005%, significance is indicated by ▲, P-value < 0.05 is indicated by ▲, P-value < 0.01 is indicated by ▲▲; vs sandalwood seed oil -0.005%, significance is indicated by ▼, P-value < 0.05 is indicated by ▼, P-value < 0.01 is indicated by ▼▼"

[0085] Analyze the data in Table 2:

[0086] 1. Targeting the inflammatory factor IL-1α:

[0087] All five groups of tested samples effectively downregulated UVB-induced elevated IL-1α gene expression levels. Among them, the inhibitory activity of the three compound combinations in groups 3 (1:1), 4 (4:1), and 5 (1:4) was significantly better than that of lactoferrin or sandalwood seed oil alone at the same concentration, with significant differences. This result confirms that when the mass ratio of lactoferrin to sandalwood seed oil is in the range of (1-4):(1-4), the combination of the two can produce a synergistic inhibitory effect, significantly enhancing the downregulation of the upstream inflammatory factor IL-1α.

[0088] 2. Targeting the inflammatory factor IL-8:

[0089] All five groups of samples exhibited activity in downregulating IL-8 gene expression. Among them, the combination in group 3 (1:1) showed a significantly better inhibitory effect on IL-8 than the two single components, and the inhibitory effect in group 4 (4:1) was significantly better than that in the group containing only sandalwood seed oil. This indicates that the synergistic effect of the two components in blocking the expression of the downstream chemokine IL-8 is most prominent at a 1:1 ratio.

[0090] 3. Targeting the antioxidant gene Nrf2:

[0091] All five groups of samples were able to upregulate Nrf2 gene expression and activate the skin's endogenous antioxidant switch. Among them, the combination of group 3 (1:1) and group 4 (4:1) showed significantly better Nrf2 enhancement than the group with sandalwood seed oil alone, and the antioxidant pathway activation ability of the combination system was significantly improved.

[0092] 4. Targeting the antioxidant gene SOD2:

[0093] All samples upregulated SOD2 gene expression and increased intracellular manganese superoxide dismutase expression. The promoting effects of group 3 (1:1), group 4 (4:1), and group 5 (1:4) were significantly stronger than those of the two single raw materials, demonstrating that the composition has a stable synergistic effect on SOD2 activation within the (1-4):(1-4) ratio range.

[0094] 5. Targeting the antioxidant gene CAT:

[0095] All samples promoted the expression of catalase CAT gene. Among them, the 3rd group (1:1) composition showed a significantly better effect on upregulating CAT than lactoferrin and sandalwood seed oil alone, and synergistically enhanced the antioxidant capacity of cells to clear hydrogen peroxide.

[0096] The statistical results of all tested indicators show that:

[0097] 1. The composition with a total mass concentration of 0.005% showed significantly better inhibitory effects on the inflammatory factor IL-1α and activation effects on the SOD2 antioxidant gene than lactoferrin or sea buckthorn seed oil alone at the same total concentration, under three mass ratios of lactoferrin to sea buckthorn seed oil of 1:1, 4:1, and 1:4. This demonstrates that (1-4):(1-4) is the effective synergistic ratio range of the composition of the present invention.

[0098] 2. The composition with a mass ratio of 4:1 has the advantage of strongly inhibiting IL-1α and upregulating SOD2; the composition with a mass ratio of 1:4 can also stably and synergistically downregulate IL-1α and increase SOD2 expression.

[0099] 3. The 1:1 mass ratio composition showed excellent synergistic effects on all detected indicators (IL-1α, IL-8, Nrf2, SOD2, CAT), and the inhibition of inflammatory factors and the upregulation of antioxidant genes both reached peak values, with the best overall soothing and antioxidant effects.

[0100] The above experimental results fully demonstrate that the combination of lactoferrin and sandalwood seed oil in this invention does not result in a simple additive effect, but rather a significant synergistic effect. The optimal formulation of this composition is a 1:1 mass ratio, which can simultaneously and effectively block skin inflammation pathways, fully activate endogenous antioxidant pathways in the skin, and efficiently improve skin inflammation and oxidative stress damage caused by UVB stimulation.

[0101] While the present invention has been illustrated and described with reference to certain preferred embodiments, those skilled in the art should understand that the above description is a further detailed explanation of the invention in conjunction with specific embodiments, and should not be construed as limiting the specific implementation of the invention to these descriptions. Various changes in form and detail can be made by those skilled in the art, including several simple deductions or substitutions, without departing from the spirit and scope of the invention.

Claims

1. A composition having soothing and antioxidant effects, characterized in that, The composition includes lactoferrin and sandalwood seed oil.

2. The composition according to claim 1, characterized in that, The concentration of lactoferrin is 0.001%-0.005%, and / or the concentration of sandalwood seed oil is 0.001%-0.005%, and / or the total concentration of lactoferrin and sandalwood seed oil is 0.001%-0.005%.

3. The composition according to claim 1, characterized in that, The mass ratio of lactoferrin to sandalwood seed oil is (1-4):(1-4).

4. The composition according to claim 3, characterized in that, The lactoferrin is derived from mammalian milk, and / or the sea buckthorn seed oil is derived from sea buckthorn seeds, and / or the mass ratio of lactoferrin to sea buckthorn seed oil is 1:

1.

5. Use of the composition according to any one of claims 1-4 in the preparation of an agent that inhibits the expression of inflammatory factors IL-1α and / or IL-8.

6. Use of the composition according to any one of claims 1-4 in the preparation of an formulation that promotes the expression of at least one of the antioxidant-related genes Nrf2, SOD2 and CAT.

7. A topical skin agent, characterized in that, Includes the composition as described in any one of claims 1-4.

8. The topical skin agent as described in claim 7, characterized in that, The topical skin agent is selected from: face cream, lotion, gel, toner, serum, face mask, eye cream, aerosol cleansing foam, shower gel, or facial cleanser.

9. Application of lactoferrin in the preparation of formulations that inhibit the expression of inflammatory factors IL-1α and / or IL-8.

10. Application of lactoferrin in the preparation of formulations that promote the expression of at least one of the antioxidant-related genes Nrf2, SOD2 and CAT.

11. Application of sea buckthorn seed oil in the preparation of formulations that inhibit the expression of inflammatory factors IL-1α and / or IL-8.

12. Application of sea buckthorn seed oil in the preparation of formulations that promote the expression of at least one of the antioxidant-related genes Nrf2, SOD2 and CAT.

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