Quantum dot synergistic anti-hair loss and whitening essential oil, its preparation method and application

Abstract

This invention discloses a quantum dot synergistic anti-hair loss and whitening essential oil, its preparation method, and its application, belonging to the field of cosmetic biomaterials technology. The essential oil comprises, by weight, 10-25 parts of herbal plant extract, 2-8 parts of amino acid chelated mineral element complex, 0.5-2 parts of water-soluble active peptide complex, 0.05-0.08 parts of near-infrared quantum dots modified with a lecithin-cholesterol lipid system, 60.02-85.05 parts of plant essential oil matrix, and 0.5-3 parts of solubilizing, emulsifying, and preservative components; the modified quantum dots have a particle size of 2-10 nm, and their resonance frequency is precisely controlled to 10¹²-10¹¹. 4 Hz. This invention constructs a quantum dot-enhanced delivery system to achieve a synergistic effect of transdermal enhancement, nutrient replenishment, and antioxidant protection. The transdermal absorption efficiency is increased by 22% compared to traditional formulas, and the hair follicle targeted utilization rate is increased by 151.9%. It has been verified by a randomized double-blind controlled trial in accordance with the "Cosmetic Efficacy Claim Evaluation Standard" that the anti-hair loss and white hair reversal effects are significant. It is suitable for the research and development and production of topical scalp care products.

Description

Technical Field

[0001] This invention relates to the field of cosmetic biomaterials technology, and in particular to a quantum dot synergistic type of anti-hair loss and anti-darkening essential oil, its preparation method and application. Background Technology

[0002] Hair loss and graying have become global health and beauty concerns, with causes encompassing multiple factors including genetics, endocrine disorders, insufficient hair follicle nutrition, scalp microcirculation disorders, and oxidative stress damage. Existing products for preventing gray hair and reversing gray hair primarily use plant extracts, water-soluble vitamins, and nutritional supplements as core ingredients, working through simple nutrient replenishment or stimulating hair follicles. However, these products generally suffer from limitations such as low transdermal absorption efficiency of active ingredients, poor targeting of hair follicles, and limited efficacy, failing to address the root causes of issues like hair follicle atrophy and insufficient melanin cell activity.

[0003] Quantum dot materials, with their unique optical properties and resonance effects, have shown great potential in targeted delivery and component tracking in the biomedical field. There are existing reports of quantum dots being used in hair loss prevention and hair growth products; for example, Chinese patent CN114129530A discloses a graphene quantum dot hair growth composition that promotes scalp blood circulation through the photothermal effect of graphene quantum dots. However, this technical solution has the following drawbacks: ① The quantum dots are not surface-modified, resulting in poor compatibility with aqueous / alcoholic matrices and easy aggregation, leading to insufficient product stability; ② The resonance frequency of the quantum dots is not precisely controlled, making it impossible to utilize the resonance effect for transdermal enhancement; ③ It relies solely on the single effect of quantum dots, failing to form a multi-dimensional synergistic system with herbal extracts, amino acid chelated minerals, and active peptides, thus limiting the improvement in efficacy.

[0004] Therefore, there is an urgent need to develop a product that combines the triple effects of transdermal enhancement, targeted empowerment, and nutritional supplementation, with excellent compatibility and stability, and also has research and development tracking value, in order to break through the existing technological bottlenecks. Summary of the Invention

[0005] In view of this, the present invention provides a quantum dot synergistic anti-hair loss and white-to-black essential oil, its preparation method and application. By constructing a quantum dot synergistic delivery system, combined with herbal extracts, amino acid chelated trace elements and water-soluble vitamins, a synergistic effect of transdermal enhancement, nutritional supplementation and antioxidant protection is achieved, significantly improving the anti-hair loss and white-to-black effect, while retaining the ability to visualize and track the ingredients, taking into account product safety and research and development value, and overcoming the defects of existing anti-hair loss and white-to-black products such as single efficacy, low transdermal efficiency, poor targeting and insufficient compatibility of nanomaterials.

[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solution: The first aspect of this invention provides a quantum dot synergistic anti-hair loss / whitening essential oil, which, by weight, comprises the following raw material components: 10-25 parts of herbal plant extract, 2-8 parts of amino acid chelated mineral element complex, 0.5-2 parts of water-soluble active peptide complex, 0.05-0.08 parts of modified quantum dots, 60.02-85.05 parts of plant essential oil matrix, and 0.5-3 parts of solubilizing, emulsifying and preservative components.

[0007] Furthermore, the herbal plant extract is one or more of the following: Platycladus orientalis leaf extract, Polygonum multiflorum extract, Rehmannia glutinosa root extract, Salvia miltiorrhiza root extract, Astragalus membranaceus extract, and Centella asiatica extract; Furthermore, the herbal extract is prepared using a low-temperature extraction process.

[0008] Furthermore, the amino acid chelated mineral element complex contains a variety of trace elements, which exist in the form of amino acid chelates. The trace elements include, but are not limited to, zinc, selenium, iron, silicon, manganese, and copper.

[0009] Furthermore, the water-soluble active peptide complex is a mixture of various biotin, tripeptide-1 copper, tripeptide-1, and acetyl hexapeptide-8; Furthermore, the water-soluble active peptide complex is composed of biotin, tripeptide-1, and acetyl hexapeptide-8 in a mass ratio of 2:1:1.

[0010] Furthermore, the modified quantum dots are near-infrared graphene quantum dots or carbon quantum dots modified by solvent coating, with a particle size of 2-10 nm and a resonance frequency tuned to 10¹²-10¹². 4 Hz.

[0011] Resonance frequency modulation basis and method: Different resonance frequencies (10¹) were screened through in vitro transdermal experiments. 0 Hz, 10¹¹Hz, 10¹²Hz, 10¹³Hz, 10¹ 4 Hz, 10¹ 5 The transdermal efficiency of quantum dots (Hz) was 10¹²-10¹⁰. 4 The quantum dots in the Hz range have the highest matching degree with the physiological metabolic frequency of hair follicle cells (about 10¹³ Hz), which can generate a resonance effect to gently open the gaps in the stratum corneum of the skin, and the transdermal efficiency is improved by more than 15% compared with other frequencies. This frequency range can be precisely controlled by adjusting the hydrothermal reaction temperature (180-220℃), reaction time (6-12h) and precursor concentration (0.1-0.5mol / L), and the photoluminescence quantum yield is ≥30%.

[0012] Furthermore, the plant essential oil matrix is ​​at least one of water, ethanol, butanediol, 1,2-hexanediol, propylene glycol, and glycerin, and does not contain silicone oil or chemical thickeners.

[0013] The numerical range derivation logic is as follows: The range of 60.02-85.05 parts is determined based on the minimum effective addition amount of each active ingredient and the maximum carrying capacity of the system: the minimum effective amount of herbal extract is 10 parts, amino acid chelated mineral elements are 2 parts, water-soluble active peptides are 0.5 parts, modified quantum dots are 0.05 parts, and the minimum solubilizing, emulsifying and preservative components are 0.43 parts. The minimum total of the above components is 12.98 parts, corresponding to a maximum matrix value of 87.02 parts. Considering the system stability requirements (active components are prone to precipitation when the matrix ratio is less than 60%), the final matrix range is determined to be 60.02-85.05 parts to ensure that each component is fully dissolved and the system is stable in the long term.

[0014] Furthermore, it also includes solubilizing, emulsifying, and preservative components; Furthermore, the dosage range of each component is as follows: polysorbate-80 0.1-1 part, p-hydroxyacetophenone 0.2-0.8 part, ethylhexylglycerin 0.1-0.5 part, phenoxyethanol 0.1-0.5 part, and capryloyl hydroxamic acid 0-0.2 part.

[0015] The second aspect of this invention provides a method for preparing the quantum dot synergistic anti-hair loss and anti-darkening essential oil described in the first aspect, comprising the following preparation steps: S1. Preparation of herbal plant extracts: Herbal plant raw materials are pulverized and herbal plant extracts are prepared using a low-temperature extraction process. S2. Preparation of modified quantum dot particles: ① Graphene quantum dots or carbon quantum dots are prepared by hydrothermal method, purified, freeze-dried, and then prepared into a quantum dot aqueous solution of 0.1-0.2 mg / mL; ② Take 5-10 parts of plant essential oil matrix, add lecithin and cholesterol, disperse, remove residual solvent by rotary evaporation to form a lipid film; ③ Add the quantum dot aqueous solution to the lipid film, sonicate for 40-60 min under ice bath conditions, sonicate at 100W for 40-60 min, extrude through a 200 nm polycarbonate membrane more than 21 times under a pressure of 0.3-0.5 MPa, dialyze through a dialysis membrane with a molecular weight cutoff of 8000 Da for 24 h for purification, and then freeze-dry to obtain modified quantum dot particles; The extrusion parameters are based on the following experimental results: When the extrusion is performed more than 21 times, the particle size distribution coefficient (PDI) of the quantum dot particles is ≤0.1, and the particle size uniformity is optimal. Further increasing the number of extrusions to 30 times does not significantly change the PDI (P>0.05), so the extrusion is limited to more than 21 times. The extrusion pressure is controlled at 0.3-0.5MPa to avoid membrane rupture and ensure that the particles can pass through the membrane pores to achieve uniformity.

[0016] S3. Compound Mixing: Add the herbal plant extract obtained in step S1, amino acid chelated mineral element complex, water-soluble active peptide complex, polysorbate-80, p-hydroxyacetophenone, ethylhexylglycerin, phenoxyethanol, and capryloyl hydroxamic acid to the remaining plant essential oil matrix. Stir at 40-50℃ for 1-2 hours until the system is homogeneous. After cooling to room temperature, add modified quantum dot particles and sonicate at 50W for 10-15 minutes until completely dispersed to obtain the anti-whitening and anti-blackening essential oil.

[0017] Further, in step S2 ②, the molar ratio of lecithin to cholesterol is (3-5):1.

[0018] Further, in step S1, after pulverizing the herbal plant raw material, add 5-10 times the mass of 60-70% ethanol aqueous solution, and extract at low temperature for 2-4 hours under the conditions of 30-40℃ and vacuum degree of -0.06~-0.08MPa. Filter to remove residue, concentrate by rotary evaporation to a solid content of 15-20%, and cool to room temperature to obtain the herbal plant extract, which is then sealed for later use.

[0019] The third aspect of this invention provides the application of the anti-hair loss and anti-whitening essential oil described in the first aspect and the anti-hair loss and anti-whitening essential oil prepared by the preparation method described in the second aspect in scalp care.

[0020] Furthermore, the hair loss prevention and whitening essential oil can be applied to scalp care products; Furthermore, when using it, apply an appropriate amount of the anti-hair loss and whitening essential oil to the scalp, massage for 3-5 minutes until absorbed, 1-2 times a day.

[0021] The present invention has the following beneficial effects.

[0022] 1. The modified quantum dots incorporated in this invention achieve targeted delivery, improving both transdermal penetration and targeting; the resonant frequency of the modified quantum dots is tuned to 10¹²-10¹². 4 Hz, quantum dot 10¹²-10¹ 4 The Hz resonance effect can gently open the gaps in the stratum corneum of the skin, increasing transdermal absorption efficiency by more than 20% compared to traditional herbal scalp care compositions, and increasing the targeted utilization rate of hair follicles by more than 35%. 2. The modified quantum dots of this invention are coated and modified by a multi-solvent-lipid system. The compatibility of the modified quantum dots with aqueous and alcoholic matrices is improved to over 99%. After being stored at room temperature in a sealed container for 6 months, there is no stratification, no particle precipitation, and no turbidity or discoloration. This improves the compatibility of quantum dots with plant essential oil matrices, ensuring that the quantum dots are stably dispersed in the plant essential oil matrices and are not prone to aggregation, thus significantly improving the product stability of the anti-whitening-to-blackening essential oil. 3. This invention utilizes a combination of multi-herb extracts, water-soluble active peptide complexes, mineral elements, and moisturizing and preservative additives to achieve synergistic effects of multiple components and comprehensive upgrades in efficacy. It can not only improve androgenetic alopecia and nutritional alopecia, but also regulate melanocyte metabolism, improve age-related gray hair and premature gray hair, while regulating the balance of scalp flora and relieving scalp discomfort. 4. This invention retains the fluorescence properties of quantum dots, which can be used to track the metabolic pathways of active ingredients through fluorescence imaging; the formulation system is mild and non-irritating, with no additional fragrances or irritating preservatives added, making it suitable for all types of scalp and skin, and has strong universality. Detailed Implementation

[0023] This invention discloses a quantum dot synergistic anti-hair loss and anti-whitening essential oil, its preparation method, and its application. Those skilled in the art can refer to the content of this document and appropriately modify the process parameters to achieve the desired result. It is particularly important to note that all similar substitutions and modifications are obvious to those skilled in the art and are considered to be included in this invention. The methods and applications of this invention have been described through preferred embodiments. Those skilled in the art can clearly modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit, and scope of this invention to realize and apply the technology of this invention.

[0024] It should be understood that the expression “one or more of…” individually includes each of the objects described after the expression, as well as various different combinations of two or more of the described objects, unless otherwise understood from the context and usage. The expression “and / or” combined with three or more described objects should be understood to have the same meaning, unless otherwise understood from the context.

[0025] The terms “including,” “having,” or “containing,” including the use of their grammatical synonyms, should generally be understood as open-ended and non-restrictive, for example, not excluding other unstated elements or steps, unless otherwise specifically stated or understood from the context.

[0026] It should be understood that the order of steps or the sequence of actions is not important as long as this application remains operational. Furthermore, two or more steps or actions can be performed simultaneously.

[0027] The use of any and all instances or exemplary language such as “e.g.” or “include” in this document is intended merely to better illustrate the application and does not constitute a limitation on the scope of the application. No language in this specification should be construed as indicating that any unclaimed element is essential to the practice of this application.

[0028] Furthermore, the numerical ranges and parameters used to define this application are approximate values, and the relevant values ​​in the specific embodiments have been presented as precisely as possible. However, any numerical value inevitably contains standard deviations due to individual test methods. Therefore, unless otherwise explicitly stated, it should be understood that all ranges, quantities, values, and percentages used in this disclosure are modified with the word "approximately." Here, "approximately" generally means that the actual value is within plus or minus 10%, 5%, 1%, or 0.5% of a specific value or range.

[0029] The first aspect of this invention provides a quantum dot synergistic anti-hair loss / whitening essential oil, which, by weight, comprises the following raw material components: 10-25 parts of herbal plant extract, 2-8 parts of amino acid chelated mineral element complex, 0.5-2 parts of water-soluble active peptide complex, 0.05-0.08 parts of modified quantum dots, and 60.02-85.05 parts of plant essential oil matrix.

[0030] Optionally, the herbal plant extract can be 15-25 parts, 20-25 parts, or 23-25 ​​parts; the amino acid chelated mineral element complex can be 2-6 parts, 2-5 parts, or 3-5 parts; the water-soluble active peptide complex can be 0.5-2 parts, or 0.5-1.5 parts, 0.5-1.2 parts, 1-2 parts, or 0.8-1 parts; the modified quantum dots can be 0.05-0.07 parts, 0.05-0.06 parts, or 0.06-0.08 parts; and the plant essential oil matrix can be 60-80 parts, 70-80 parts, 70-78 parts, 75-78 parts, or 78-79 parts.

[0031] Furthermore, the herbal plant extract is one or more of the following: Platycladus orientalis leaf extract, Polygonum multiflorum extract, Rehmannia glutinosa root extract, Salvia miltiorrhiza root extract, Astragalus membranaceus extract, and Centella asiatica extract; Furthermore, the herbal plant extract is prepared using a low-temperature extraction process to retain active ingredients such as plant polyphenols, flavonoids, and saponins.

[0032] Preferably, the herbal plant extract is an extract of Platycladus orientalis leaves, Polygonum multiflorum, Rehmannia glutinosa root, and Salvia miltiorrhiza root, wherein the weight ratio of Platycladus orientalis leaves, Polygonum multiflorum, Rehmannia glutinosa root, and Salvia miltiorrhiza root is 15:5:3:2.

[0033] Preferably, the herbal plant extract is an extract of Platycladus orientalis leaves, Polygonum multiflorum, Astragalus membranaceus and Centella asiatica, and the weight ratio of Platycladus orientalis leaves, Polygonum multiflorum, Astragalus membranaceus and Centella asiatica is 10:8:3:2.

[0034] Furthermore, the amino acid chelated mineral element complex contains a variety of trace elements, which exist in the form of amino acid chelates. The trace elements include, but are not limited to, zinc, selenium, iron, silicon, manganese, and copper.

[0035] Understandably, the bioavailability of amino acid chelated mineral element complexes is more than 40% higher than that of inorganic mineral salts. Silicon can strengthen hair strands, increase hair thickness and resilience, reinforce hair follicle connective tissue, reduce hair breakage and loss, participate in keratin synthesis, improve dry and brittle hair, keep the scalp healthy, and promote hair growth by improving microcirculation. Copper can promote melanin production and prevent gray hair, while maintaining normal hair follicle metabolism. Copper deficiency can easily lead to hair follicle atrophy, hair loss, and premature graying. Manganese has antioxidant properties, protects hair follicle cells, promotes scalp blood circulation, and participates in hair keratin synthesis. Deficiency can lead to brittle hair and diffuse hair loss. It can also reduce oxidative damage to hair follicles caused by ultraviolet rays and inflammation. Zinc affects melanin synthesis, and its antioxidant properties can protect hair follicles from free radical damage; it can also inhibit 5α-reductase activity, which may help improve androgenetic alopecia. Furthermore, the water-soluble active peptide complex is a mixture of various biotin, tripeptide-1 copper, tripeptide-1, and acetyl hexapeptide-8; Furthermore, the water-soluble active peptide complex is composed of biotin, tripeptide-1, and acetyl hexapeptide-8 in a mass ratio of 2:1:1, and can directly participate in the metabolism of hair follicle cells and the process of melanin synthesis.

[0036] In this technical solution, biotin can promote keratin synthesis and repair damaged hair follicles; tripeptide and hexapeptide active ingredients regulate the hair follicle life cycle and melanin secretion.

[0037] Furthermore, the modified quantum dots are near-infrared graphene quantum dots or carbon quantum dots modified by solvent coating, with a particle size of 2-10 nm and a resonance frequency tuned to 10¹²-10¹². 4 Hz.

[0038] In this technical solution, the resonant frequency of the modified quantum dots is tuned to 10¹²-10¹. 4 Hz, matching the physiological metabolic frequency of hair follicle cells, with a photoluminescence quantum yield ≥30%, making quantum dots 10¹²-10¹ 4The Hz resonance effect can gently open the gaps in the stratum corneum of the skin, increasing transdermal absorption efficiency by more than 20% compared to traditional herbal scalp care compositions, and increasing the targeted utilization rate of hair follicles by more than 35%.

[0039] Furthermore, the plant essential oil matrix is ​​at least one of water, ethanol, butylene glycol, 1,2-hexanediol, propylene glycol, and glycerin.

[0040] In this technical solution, polyols and herbal extracts work together to eliminate free radicals on the scalp, reduce oxidative stress damage to hair follicles, and the multi-component synergy increases the efficiency of turning gray hair black by more than 10%, while also improving dry, sensitive, and red scalp conditions.

[0041] Furthermore, it also includes solubilizing, emulsifying, and preservative components; Furthermore, the solubilizing, emulsifying, and preservative components are one or more of polysorbate-80, p-hydroxyacetophenone, ethylhexylglycerin, phenoxyethanol, and capryloyl hydroxamic acid.

[0042] In this technical solution, the solubilizing, emulsifying, and preservative components work synergistically to stabilize the system formulation, further improving the stability of the product.

[0043] The second aspect of this invention provides a method for preparing the quantum dot synergistic anti-hair loss and anti-darkening essential oil described in the first aspect, comprising the following preparation steps: S1. Preparation of herbal plant extracts: Herbal plant raw materials are pulverized and herbal plant extracts are prepared using a low-temperature extraction process. S2. Preparation of modified quantum dot particles: ① Graphene quantum dots or carbon quantum dots are prepared by hydrothermal method, purified, freeze-dried, and then prepared into a quantum dot aqueous solution of 0.1-0.2 mg / mL; ② Take 5-10 parts of plant essential oil matrix, add lecithin and cholesterol, disperse, remove residual solvent by rotary evaporation to form a lipid film; ③ Add the quantum dot aqueous solution to the lipid film, sonicate for 40-60 min under ice bath conditions, extrude through a polycarbonate membrane, purify, and freeze-dry to obtain modified quantum dot particles; S3. Compound Mixing: Add the herbal plant extract obtained in step S1, amino acid chelated mineral element complex, water-soluble active peptide complex, polysorbate-80, p-hydroxyacetophenone, ethylhexylglycerin, phenoxyethanol, and capryloyl hydroxamic acid to the remaining plant essential oil matrix. Stir at 40-50℃ for 1-2 hours until the system is homogeneous. After cooling to room temperature, add modified quantum dot particles and sonicate at 50W for 10-15 minutes until completely dispersed to obtain the anti-whitening and anti-blackening essential oil.

[0044] Further, in step S2 ②, the molar ratio of lecithin to cholesterol is (3-5):1.

[0045] Further, in step S1, after pulverizing the herbal plant raw material, add 5-10 times the mass of 60-70% ethanol aqueous solution, and extract at low temperature for 2-4 hours under the conditions of 30-40℃ and vacuum degree of -0.06~-0.08MPa. Filter to remove residue, concentrate by rotary evaporation to a solid content of 15-20%, and cool to room temperature to obtain the herbal plant extract, which is then sealed for later use.

[0046] Further, in step S2 ①, the purification operation is to dialyze the material through a dialysis bag with a molecular weight cutoff of 500 Da for 48 hours; in step S2 ②, the dispersion operation is to disperse the material evenly by sonication at 100W for 5-10 minutes, and the rotary evaporation to remove residual solvent is carried out at a temperature of 40℃ for 15-60 minutes; in step S2 ③, the particles are homogenized by extrusion through a 200nm polycarbonate membrane more than 21 times, and the purification operation is to dialyze the material through a dialysis membrane with a molecular weight cutoff of 8000 Da for 24 hours.

[0047] The third aspect of this invention provides the application of the anti-hair loss and anti-whitening essential oil described in the first aspect and the anti-hair loss and anti-whitening essential oil prepared by the preparation method described in the second aspect in scalp care.

[0048] Furthermore, the hair loss prevention and whitening essential oil can be applied to scalp care products; Furthermore, when using it, apply an appropriate amount of the anti-hair loss and whitening essential oil to the scalp, massage for 3-5 minutes until absorbed, 1-2 times a day.

[0049] Unless otherwise specified, the raw materials, reagents, consumables and instruments involved in this invention are all commercially available products and can be purchased from the market.

[0050] The present invention will be further illustrated below with reference to the embodiments.

[0051] Example 1

[0052] A quantum dot synergistic essential oil for preventing gray hair from turning black, comprising the following raw material components by weight: 28 parts of herbal plant extracts (15 parts of Platycladus orientalis leaf extract, 5 parts of Polygonum multiflorum extract, 3 parts of Rehmannia glutinosa root extract, and 2 parts of Salvia miltiorrhiza root extract), 5 parts of amino acid chelated mineral element complex, 1 part of water-soluble active peptide complex (a mixture of biotin, tripeptide-1, and acetyl hexapeptide-8), 0.06 parts of modified graphene quantum dots, and 78.94 parts of plant essential oil matrix (a mixture of water, ethanol, and butylene glycol).

[0053] The preparation method of the hair loss prevention and anti-whitening essential oil includes the following preparation steps: S1. Preparation of herbal plant extracts: After mixing and pulverizing the raw materials of Platycladus orientalis leaves, Polygonum multiflorum, Rehmannia glutinosa root and Salvia miltiorrhiza root, add 8 times the mass of 65% ethanol aqueous solution, extract at low temperature for 3h at 35℃ and vacuum degree -0.07MPa, filter to remove residue, concentrate to solid content of 18%, cool and seal for later use.

[0054] S2. Preparation of modified quantum dot particles: ① Graphene quantum dots were prepared by hydrothermal method, purified by dialysis with a molecular weight cutoff of 500 Da for 48 h, and then prepared into a quantum dot aqueous solution of 0.15 mg / mL; ② Take 8 parts of plant essential oil matrix (a mixture of water + ethanol + butanediol), add lecithin and cholesterol (molar ratio 4:1), disperse evenly by sonication at 100 W for 8 min, remove residual solvent by rotary evaporation at 40 °C for 30 min, and form a lipid film; ③ Add the quantum dot aqueous solution to the lipid film, sonicate at 100 W for 50 min under ice bath conditions, extrude 25 times through a 200 nm polycarbonate membrane, then dialyze through an 8000 Da dialysis membrane for 24 h, and freeze-dry to obtain modified quantum dot particles.

[0055] S3. Compound Mixing: Add the prepared herbal plant extract, amino acid chelated mineral element complex, water-soluble active peptide complex, polysorbate-80, p-hydroxyacetophenone, ethylhexylglycerin, phenoxyethanol, and capryloyl hydroxamic acid to the remaining 70.94 parts of plant essential oil matrix. Stir at 45°C for 1.5 hours until the system is homogeneous and transparent. After cooling to room temperature, add the modified quantum dot particles and sonicate at 50W for 12 minutes until completely dispersed to obtain the finished product.

[0056] Example 2

[0057] A quantum dot synergistic essential oil for preventing gray hair from turning black, comprising the following raw material components by weight: 23 parts of herbal plant extracts (10 parts of Platycladus orientalis leaf extract, 8 parts of Polygonum multiflorum extract, 3 parts of Astragalus membranaceus extract, and 2 parts of Centella asiatica extract), 3 parts of amino acid chelated mineral element complex, 0.8 parts of water-soluble active peptide complex (a mixture of biotin and tripeptide-1 copper), 0.05 parts of modified carbon quantum dots, and 78.15 parts of plant essential oil matrix (a mixture of water, propylene glycol, and glycerin).

[0058] The preparation method of the hair loss prevention and anti-whitening essential oil includes the following preparation steps: S1. Preparation of herbal plant extracts: After mixing and pulverizing the raw materials of Platycladus orientalis leaves, Polygonum multiflorum, Astragalus membranaceus, and Centella asiatica, add 8 times the mass of 65% ethanol aqueous solution, extract at low temperature for 3 hours at 35℃ and vacuum degree -0.07MPa, filter to remove residue, concentrate to a solid content of 18%, cool and seal for later use.

[0059] S2. Preparation of modified quantum dot particles: ① Carbon quantum dots were prepared by hydrothermal method, purified by dialysis with a molecular weight cutoff of 500 Da for 48 h, and then prepared into a quantum dot aqueous solution of 0.15 mg / mL; ② Eight parts of plant essential oil matrix (a mixture of water + propylene glycol + glycerol) were taken, and lecithin and cholesterol (molar ratio 4:1) were added. The mixture was sonicated at 100 W for 8 min to disperse evenly, and then rotary evaporated at 40 °C for 30 min to remove residual solvent, forming a lipid film; ③ The quantum dot aqueous solution was added to the lipid film, and the mixture was sonicated at 100 W for 50 min under ice bath conditions. The mixture was then extruded 25 times through a 200 nm polycarbonate membrane, and then dialyzed through an 8000 Da dialysis membrane for 24 h. Finally, the modified quantum dot particles were obtained by freeze drying.

[0060] S3. Compound Mixing: Add the prepared herbal plant extract, amino acid chelated mineral element complex, water-soluble active peptide complex, polysorbate-80, p-hydroxyacetophenone, ethylhexylglycerin, phenoxyethanol, and capryloyl hydroxamic acid to the remaining 70.15 parts of plant essential oil matrix. Stir at 45℃ for 1.5h until the system is homogeneous and transparent. After cooling to room temperature, add modified quantum dot particles and sonicate at 50W for 12min until completely dispersed to obtain the finished product.

[0061] Experimental Example 1: Comparative Study of Transdermal Absorption and Hair Follicle Targeting

[0062] 1.1 Experimental Grouping

[0063] Five groups of test samples were set up. Except for the single variable, the formulation and preparation process were the same as in Example 1: Example 1 Group: Contains 0.06 parts of lipid-coated modified graphene quantum dots (resonance frequency 10¹³ Hz) Blank control group: Contains no quantum dots Control group 1: Contains 0.06 parts of unmodified graphene quantum dots Control group 2: Contains 0.06 parts of lipid-coated modified graphene quantum dots (resonance frequency 10¹¹Hz) Control group 3: Contains 0.06 parts of lipid-coated modified graphene quantum dots (resonance frequency 10¹). 5 Hz) 1.2 Test Methods The Franz diffusion cell method was used, with isolated pig scalp as the transdermal barrier and pH 7.4 phosphate buffer as the receiving solution. The mixture was stirred at 37°C and samples were taken at 2h, 4h, 6h, 8h, and 12h. The total flavonoid content in the receiving solution (representing the transdermal transdermal amount of active ingredients) was determined by high performance liquid chromatography. After 12h, hair follicles were separated from the scalp, and the fluorescence intensity of quantum dots in the hair follicles was quantitatively analyzed by fluorescence microscopy to calculate the targeted utilization rate of the hair follicles.

[0064] 1.3 Test Results

[0065] Results analysis: Unmodified quantum dots (control group 1) could only slightly improve transdermal efficiency and targeting, indicating that surface modification is the basis for the function of quantum dots; The resonant frequency deviates from 10¹²-10¹ 4 The modified quantum dots of Hz (control groups 2 and 3) showed significantly lower performance than those in Example 1, demonstrating that 10¹²-10¹ 4 Hz resonant frequency is a key technical feature for achieving transdermal enhancement and targeted delivery; In Example 1, the transdermal efficiency of the group was increased by 22% compared with the blank group, and the hair follicle targeting utilization rate was increased by 151.9%, which verified the synergistic technical contribution of lipid encapsulation modification and resonance frequency regulation.

[0066] Test Example 2: Product Stability Comparison Test

[0067] The above 5 groups of samples were stored at room temperature in a sealed container for 6 months, and the changes in appearance were observed monthly. The results are as follows:

[0068] The results showed that lipid coating modification could improve the compatibility of quantum dots with the matrix to over 99%, solving the core problems of easy aggregation and poor stability of unmodified quantum dots.

[0069] Example 3: Trial trial conducted in accordance with the "Evaluation Standards for Cosmetic Efficacy Claims"

[0070] 3.1 Experimental Design

[0071] In accordance with the requirements of Chapter 6 "Evaluation of Hair Loss Prevention Efficacy" and Chapter 12 "Evaluation of Other Efficacy" of the "Guidelines for Evaluation of Cosmetic Efficacy Claims" (2021 Edition), a randomized, double-blind, placebo-controlled trial design was adopted: Subjects: 120 eligible volunteers were recruited, including 60 volunteers with hair loss (30 men and 30 women, aged 18-50, with a daily hair loss of ≥50 strands) and 60 volunteers with gray hair (30 men and 30 women, aged 25-60, with gray hair accounting for 10%-50%). Volunteers with scalp diseases, those who had used anti-hair loss and gray hair reversal products in the past 3 months, and those who were taking medications that affect hair growth were excluded.

[0072] Grouping: Volunteers with hair loss and those with gray hair were randomly divided into an experimental group and a control group, with 30 people in each group; the experimental group used the essential oil prepared in Example 1, and the control group used a placebo essential oil without modified quantum dots.

[0073] Directions for use: Apply 2mL of essential oil to the scalp once in the morning and once in the evening, massage for 3-5 minutes until absorbed, and use continuously for 6 months. During the trial period, do not use other hair loss prevention, hair growth, or hair dye products, and maintain a normal diet and lifestyle.

[0074] Evaluation indicators: Hair loss prevention effect: Hair density and daily hair loss in a fixed area on the top of the subject's head were measured using a hair image analyzer at weeks 0, 12, and 24. White to black effect: At weeks 0 and 24, the proportion of white hair in a fixed area on the top of the head of the subjects was measured by image analysis, and the rate of white hair turning black at the root was counted. Safety: Record adverse reactions such as scalp itching, redness, and stinging that occurred during the trial.

[0075] 3.2 Test Results

[0076] Comparison of anti-hair loss effects (n=30):

[0077] Comparison of white-to-black effect (n=30):

[0078] Safety results: During the trial, only 2 cases of mild scalp itching occurred in the experimental group and 1 case of mild scalp redness occurred in the control group. All cases resolved spontaneously without special treatment. There was no statistically significant difference in the incidence of adverse reactions between the two groups (P>0.05).

[0079] Results analysis: The experimental group experienced a 45.1% reduction in daily hair loss and a 26.5% increase in hair density over 24 weeks, which was significantly better than the control group (P<0.001), demonstrating that the modified quantum dot synergistic system can significantly improve the effects of preventing hair loss and promoting hair growth. The rate of gray hair turning black at the root in the experimental group after 24 weeks reached 42.3%, which was significantly higher than 11.7% in the control group (P<0.001). The result was statistically significant, excluding the influence of age, diet and other interfering factors, and verifying the reproducibility of the product's gray-to-black effect. The product has good safety, with no serious adverse reactions occurring, and is suitable for all types of scalp and skin.

[0080] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A quantum dot synergistic effect anti-hair loss and white-to-black essential oil, characterized in that, By weight, the anti-hair loss and anti-darkening essential oil comprises the following raw material components: 10-25 parts of herbal plant extract, 2-8 parts of amino acid chelated mineral element complex, 0.5-2 parts of water-soluble active peptide complex, 0.05-0.08 parts of modified quantum dots, 60.02-85.05 parts of plant essential oil matrix, and 0.5-3 parts of solubilizing, emulsifying and preservative components.

2. The quantum dot synergistic effect type anti-hair loss and white-to-black essential oil according to claim 1, characterized in that, The herbal plant extract is one or more selected from the following: Platycladus orientalis leaf extract, Polygonum multiflorum extract, Rehmannia glutinosa root extract, Salvia miltiorrhiza root extract, Astragalus membranaceus extract, and Centella asiatica extract; and / or, The herbal plant extract was prepared using a low-temperature extraction process.

3. The quantum dot synergistic effect type anti-hair loss and white-to-black essential oil according to claim 1, characterized in that, The amino acid chelated mineral element complex contains a variety of trace elements, which exist in the form of amino acid chelates. The trace elements include one or more of zinc, selenium, iron, silicon, manganese, and copper.

4. The quantum dot synergistic effect type anti-hair loss and white-to-black essential oil according to claim 1, characterized in that, The water-soluble active peptide complex is a mixture of various biotin, tripeptide-1 copper, tripeptide-1, and acetyl hexapeptide-8.

5. The quantum dot synergistic effect type anti-hair loss and white-to-black essential oil according to claim 1, characterized in that, The modified quantum dots are near-infrared band graphene quantum dots or carbon quantum dots modified by lecithin-cholesterol lipid system, the particle size is 2-10nm, the resonance frequency is regulated to 10 4 Hz, and the photoluminescence quantum yield is greater than or equal to 30%.

6. The quantum dot synergistic effect type anti-hair loss and white-to-black essential oil according to claim 1, characterized in that, The plant essential oil matrix is ​​at least one of water, ethanol, butylene glycol, 1,2-hexanediol, propylene glycol, and glycerin.

7. The quantum dot synergistic effect type anti-hair loss and white-to-black essential oil according to claim 1, characterized in that, The solubilizing, emulsifying, and preservative components are one or more of polysorbate-80, p-hydroxyacetophenone, ethylhexylglycerin, phenoxyethanol, and capryloyl hydroxamic acid; the dosage range of each component is: polysorbate-80 0.1-1 part, p-hydroxyacetophenone 0.2-0.8 parts, ethylhexylglycerin 0.1-0.5 parts, phenoxyethanol 0.1-0.5 parts, and capryloyl hydroxamic acid 0-0.2 parts.

8. The preparation method of the quantum dot synergistic anti-hair loss and whitening essential oil according to any one of claims 1-7, characterized in that, The preparation steps include the following: S1. Preparation of herbal plant extracts: Herbal plant raw materials are pulverized and herbal plant extracts are prepared using a low-temperature extraction process. S2. Preparation of modified quantum dot particles: ① Graphene quantum dots or carbon quantum dots are prepared by hydrothermal method, purified, freeze-dried, and then prepared into a quantum dot aqueous solution of 0.1-0.2 mg / mL; ② Take 5-10 parts of plant essential oil matrix, add lecithin and cholesterol, disperse, remove residual solvent by rotary evaporation to form a lipid film; ③ Add the quantum dot aqueous solution to the lipid film, sonicate for 40-60 min under ice bath conditions, extrude through a 200 nm polycarbonate membrane more than 21 times under a pressure of 0.3-0.5 MPa, dialyze through a dialysis membrane with a molecular weight cutoff of 8000 Da for 24 h for purification, and then freeze-dry to obtain modified quantum dot particles; S3. Compound Mixing: Add the herbal plant extract obtained in step S1, amino acid chelated mineral element complex, water-soluble active peptide complex, polysorbate-80, p-hydroxyacetophenone, ethylhexylglycerin, phenoxyethanol, and capryloyl hydroxamic acid to the remaining plant essential oil matrix. Stir at 40-50℃ for 1-2 hours until the system is homogeneous. After cooling to room temperature, add modified quantum dot particles and sonicate at 50W for 10-15 minutes until completely dispersed to obtain the anti-whitening and anti-blackening essential oil.

9. The preparation method of the quantum dot synergistic anti-hair loss and anti-darkening essential oil as described in claim 8, characterized in that, In step S2, ②, the molar ratio of lecithin to cholesterol is (3-5):

1.

10. The application of the anti-hair loss and anti-whitening essential oil as described in any one of claims 1-7, and the anti-hair loss and anti-whitening essential oil prepared by the preparation method described in any one of claims 8-9, in scalp care.

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