A water-oil two-phase composition and its use in body oils

Abstract

This invention discloses a two-phase water-oil repair and moisturizing active composition, belonging to the field of daily chemical product technology. The active composition includes an oil-phase active ingredient, a fat-soluble extract of Phellodendron amurense, and an aqueous-phase active ingredient, a water-soluble extract of mulberry leaf and a water-soluble extract of Artemisia argyi. The fat-soluble extract of Phellodendron amurense is prepared by extraction using fermented red pine nut oil as a solvent. The fermented red pine nut oil is prepared using red pine nut powder as raw material and Trichoderma reesei as the fermentation strain. This invention enriches the active ingredients in red pine nut oil through fermentation with Trichoderma reesei, then uses it as a solvent to extract the fat-soluble components of Phellodendron amurense, and combines it with the water-soluble extracts of mulberry leaf and Artemisia argyi to obtain a two-phase water-oil composition with repair and moisturizing activities. The oil and aqueous phases in the composition have significant synergistic effects on multiple levels, including hydration, water retention, barrier repair, and anti-oxidation, giving the composition excellent moisturizing, skin barrier repair, and anti-oxidation effects. It is gentle and non-irritating, suitable for various skin types, and can be directly added as an active ingredient to various cosmetics.

Description

Technical Field

[0001] This invention belongs to the field of daily chemical products technology, specifically relating to a water-oil two-phase composition and its application in body oil. Background Technology

[0002] The skin barrier is the body's first line of defense against external stimuli, and its structural integrity directly affects the health of the skin. When the skin barrier is damaged, transepidermal water loss intensifies, leading to dryness, flaking, and itching. Simultaneously, external irritants can more easily penetrate, causing sensitivity and inflammation. With the fast pace of life, increased environmental pollution, and the prevalence of unhealthy skincare habits such as over-cleansing, the proportion of people with damaged skin barriers is rising year by year, and consumers are increasingly demanding body care products that combine moisturizing, barrier repair, and antioxidant effects.

[0003] Existing body care products have the following main shortcomings: First, traditional soap-based cleansers have excessive degreasing power, which can damage the skin's surface lipid film and aggravate barrier damage. Second, most ordinary body lotions are mainly water-based moisturizers, which can only achieve short-term hydration and lack long-lasting water-locking and active repair capabilities, resulting in poor moisturizing duration. Third, although single-oil products can physically lock in water, they are greasy in texture, absorb slowly, and can only replenish the skin's surface oils, failing to repair the damaged stratum corneum structure. Fourth, existing water-oil two-phase products are mostly simple physical mixtures, lacking a clear synergistic mechanism between active ingredients, making it difficult to simultaneously achieve multiple effects such as deep hydration, long-lasting water-locking, barrier repair, and anti-oxidation.

[0004] Therefore, there is an urgent need to develop a body care composition that has a clear synergistic effect of active ingredients, while also possessing excellent moisturizing, skin barrier repair, and antioxidant effects, and is gentle and non-irritating. Summary of the Invention

[0005] The purpose of this invention is to provide a water-oil two-phase composition and its application in body oils.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: In a first aspect, the present invention provides a water-oil two-phase active composition, the composition comprising active ingredients: Phellodendron amurense extract, mulberry leaf extract, and Artemisia argyi extract; Among them, the Phellodendron amurense extract is a fat-soluble extract of Phellodendron amurense, which is prepared by extraction using fermented red pine seed oil as a solvent; Among them, the mulberry leaf extract is a water-soluble extract of mulberry leaves, which is prepared by extraction with deionized water as a solvent; The Artemisia argyi extract is a water-soluble extract of Artemisia argyi, prepared by extraction with deionized water as a solvent. The fermented red pine nut oil mentioned above was prepared by fermentation using red pine nut powder as raw material and Trichoderma reesei as the strain.

[0007] Preferably, the present invention provides a method for preparing the above-mentioned fermented red pine nut oil, the specific steps of which are as follows: S1: Take dried red pine nuts, grind them into powder to 80-100 mesh, then add water at a material-to-liquid ratio of 1:5-8 (g / mL) and soak for 12-16 hours to obtain suspension A.

[0008] S2: Take suspension A and sterilize it at 121℃ for 20 minutes. After cooling to room temperature, inoculate it with Trichoderma reesei culture and ferment for 24-48 hours. The fermentation start temperature is 28-30℃. During the fermentation process, maintain an aeration rate of 0.3-7 L / min. After fermentation, irradiate it for sterilization, cool it, filter it to remove the bacterial residue, separate the liquid and take the upper oil to obtain fermented red pine nut oil.

[0009] Preferably, the inoculum size of *Trichoderma reesei* in S2 is 1-3 wt% of the mass of suspension A; the viable count of *Trichoderma reesei* in the inoculum is 1 × 10⁻⁶. 6 -1×10 8 CFU / mL.

[0010] Preferably, the present invention provides a method for preparing the above-mentioned water-oil two-phase active composition, the method comprising the following steps: Preparation of Phellodendron bark powder and fermented red pine nut oil at a mass ratio of 1:3-5, heat to 40-50℃, stir and extract for 3-5 hours, then cool to room temperature, filter out insoluble matter, and take the oil phase to obtain the Phellodendron bark powder.

[0011] Preparation of water-soluble mulberry leaf extract: Mulberry leaf powder and deionized water were mixed evenly at a mass ratio of 1:7-9, heated to 60-70℃, stirred and extracted for 2-4 hours, then cooled to room temperature, insoluble matter was filtered off, aqueous phase was collected, and freeze-dried to constant weight to obtain water-soluble mulberry leaf extract.

[0012] Preparation of Artemisia argyi water-soluble extract: Artemisia argyi powder and deionized water were mixed evenly at a mass ratio of 1:7-9, heated to 60-70℃, stirred and extracted for 2-4 hours, then cooled to room temperature, insoluble matter was filtered off, aqueous phase was collected, and freeze-dried to constant weight to obtain Artemisia argyi water-soluble extract.

[0013] Preparation of the water-oil two-phase composition: The oil phase was prepared by using the fat-soluble extract of Phellodendron chinense; the water-soluble extracts of mulberry leaves and Artemisia argyi were mixed evenly at a mass ratio of 1:(8-12) to obtain a mixed powder; the mixed powder was then prepared into a 10wt% mixed solution (i.e., 10g of the mixed powder of mulberry leaf and Artemisia argyi extracts per 100g of aqueous solution) using deionized water to obtain the aqueous phase; the oil phase and the aqueous phase were stirred and mixed at a mass ratio of 1:0.5-2 at 3000r / min for 10min to obtain the water-oil two-phase repair and moisturizing active composition.

[0014] Secondly, the present invention provides a repairing and moisturizing body oil, the raw materials of which include the following components: The above-mentioned water-oil two-phase repair and moisturizing active composition includes base oil, emulsifier, moisturizer, and deionized water.

[0015] The base oil is jojoba seed oil and / or camellia seed oil and / or sunflower seed oil; the moisturizer is propylene glycol, glycerin and / or sodium hyaluronate.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: The use of Trichoderma reesei fermentation technology to prepare red pine seed oil significantly enhances its skin repair activity, laying the foundation for the subsequent extraction of active ingredients and the exertion of their effects.

[0017] Innovatively, red pine nut oil obtained after fermentation is used as a solvent to extract fat-soluble components from Phellodendron amurense, achieving synergistic effects between the solvent and the active ingredients, and avoiding the safety hazards of organic solvents.

[0018] By scientifically combining the fat-soluble extract of Phellodendron bark with the water-soluble extracts of mulberry leaf and Artemisia argyi, and combining it with the base repairing effect of fermented red pine nut oil, the composition simultaneously possesses excellent moisturizing, skin barrier repair, and antioxidant effects.

[0019] The composition and the body oil containing it are mild and non-irritating. They have been verified to be mild and non-irritating through skin patch testing on healthy volunteers and are suitable for a variety of skin types. Detailed Implementation

[0020] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0021] Some of the raw materials and their sources are as follows: Trichoderma reesei: purchased from Ningbo Mingzhou Biotechnology Co., Ltd., product number BMZ115313; Trichoderma viride: purchased from Ningbo Mingzhou Biotechnology Co., Ltd., product number BMZ127533; Brewing yeast: purchased from Angel Yeast Co., Ltd.; Red pine nuts: dried, mature seeds, food grade, purity ≥99%, supplier: Bozhou Xier Traditional Chinese Medicine Co., Ltd.; Phellodendron bark powder: 100 mesh, food grade, purity ≥98%, supplier: Bozhou Xier Traditional Chinese Medicine Co., Ltd.; Mulberry leaf powder: 100 mesh, food grade, purity ≥98%, supplier: Bozhou Xier Traditional Chinese Medicine Co., Ltd.; Artemisia argyi powder: 100 mesh, food grade, purity ≥98%, supplier: Bozhou Xier Traditional Chinese Medicine Co., Ltd.; Cold-pressed red pine nut oil: purchased from Shanghai Ruidean Biotechnology Co., Ltd.; Jojoba seed oil: purchased from Vantage; Camellia seed oil: purchased from Shanghai Zhina Biotechnology Co., Ltd.; Sunflower seed oil: purchased from Shanghai Kerry Oils & Grains Industries Co., Ltd. Lauryl alcohol polyether-4: purchased from Sasol (China) Chemical Co., Ltd. PEG-25 hydrogenated castor oil: purchased from BASF; Unless otherwise specified, all other materials and reagents used in the examples are commercially available.

[0022] Examples 1-3: Preparation of Fermented Red Pine Nut Oil Example 1: Dried red pine nuts were ground and pulverized through a 100-mesh sieve to obtain dried red pine nut powder. Deionized water was added to the dried red pine nut powder at a material-to-liquid ratio of 1:6 (g / mL), and the mixture was soaked for 14 hours to obtain suspension A. Suspension A was sterilized at 121℃ for 20 minutes. After cooling to room temperature, Trichoderma reesei inoculum was inoculated at a rate of 2wt% of the mass of suspension A, with a viable count of 1×10⁻⁶ cells / mL. 7 The fermentation concentration was CFU / mL, the fermentation initiation temperature was controlled at 29℃, the aeration rate was maintained at 1L / min during fermentation, and the fermentation time was 36 hours. After fermentation, the mixture was sterilized by irradiation, then filtered to remove the bacterial residue. The filtrate was transferred to a separatory funnel and allowed to stand for separation. The clear oil in the upper layer was collected to obtain the fermented red pine nut oil of Example 1.

[0023] Example 2: Dried red pine nuts were ground and pulverized through a 100-mesh sieve to obtain dried red pine nut powder. Deionized water was added to the dried red pine nut powder at a material-to-liquid ratio of 1:5 (g / mL), and the mixture was soaked for 12 hours to obtain suspension A. Suspension A was sterilized at 121℃ for 20 minutes. After cooling to room temperature, Trichoderma reesei inoculum was inoculated at a rate of 1 wt% of the mass of suspension A, with a viable count of 1 × 10⁻⁶ cells / mL. 6 The fermentation concentration was CFU / mL, the fermentation initiation temperature was controlled at 28℃, the aeration rate was maintained at 1L / min during fermentation, and the fermentation time was 24 hours. After fermentation, the mixture was sterilized by irradiation, then filtered to remove the bacterial residue. The filtrate was transferred to a separatory funnel and allowed to stand for separation. The clear oil in the upper layer was collected to obtain the fermented red pine nut oil of Example 2.

[0024] Example 3: Dried red pine nuts were ground and pulverized through a 100-mesh sieve to obtain dried red pine nut powder. Deionized water was added to the dried red pine nut powder at a material-to-liquid ratio of 1:8 (g / mL), and the mixture was soaked for 16 hours to obtain suspension A. Suspension A was sterilized at 121℃ for 20 minutes. After cooling to room temperature, Trichoderma reesei inoculum was inoculated at a rate of 3 wt% of the mass of suspension A, with a viable count of 1 × 10⁻⁶ cells / mL.8 The fermentation concentration was CFU / mL, the fermentation initiation temperature was controlled at 30℃, the aeration rate was maintained at 1L / min during fermentation, and the fermentation time was 48 hours. After fermentation, the mixture was sterilized by irradiation, then filtered to remove the bacterial residue. The filtrate was transferred to a separatory funnel and allowed to stand for separation. The clear oil in the upper layer was collected to obtain the fermented red pine nut oil of Example 3.

[0025] Comparative Examples 1-3: Preparation of Control Fermented Red Pine Nut Oil / Ordinary Pressed Red Pine Nut Oil Comparative Example 1: Commercially available cold-pressed red pine nut oil.

[0026] Comparative Example 2: The fermentation strain was replaced with Trichoderma viride, and the rest of the preparation process and parameters were exactly the same as in Example 1, resulting in Trichoderma viride fermented red pine nut oil.

[0027] Comparative Example 3: The fermentation strain was replaced with brewer's yeast, and the rest of the preparation process and parameters were exactly the same as in Example 1, resulting in brewer's yeast fermented red pine nut oil.

[0028] Examples 4-6: Preparation of Water-Oil Two-Phase Repairing and Moisturizing Active Compositions Example 4: Preparation of Phellodendron bark powder: 10g of Phellodendron bark powder was mixed evenly with 40g of fermented red pine oil prepared in Example 1, heated to 45℃, and stirred for 4 hours at a stirring speed of 200r / min. The mixture was then cooled to room temperature, insoluble matter was filtered off, and the oil phase filtrate was collected to obtain the Phellodendron bark powder.

[0029] Preparation of water-soluble mulberry leaf extract: Mix 10g of mulberry leaf powder with 80g of deionized water until homogeneous, heat to 65℃, and stir for 3 hours at a stirring speed of 200r / min. Then cool to room temperature, filter out the insoluble matter, collect the aqueous filtrate, and freeze-dry it in a freeze dryer until constant weight to obtain the water-soluble mulberry leaf extract.

[0030] Preparation of water-soluble extract of Artemisia argyi: Mix 10g of Artemisia argyi powder with 80g of deionized water, heat to 65℃, and stir for 3 hours at a stirring speed of 200r / min. Then cool to room temperature, filter out the insoluble matter, collect the aqueous filtrate, and freeze-dry it in a freeze dryer until constant weight to obtain water-soluble extract of Artemisia argyi.

[0031] Preparation of the water-oil two-phase composition: The above-prepared turmeric fat-soluble extract was used as the oil phase; the mulberry leaf water-soluble extract and the artemisia argyi water-soluble extract were mixed evenly at a mass ratio of 1:10 to obtain a mixed powder. The mixed powder was then prepared into a 10wt% mixed solution (i.e., 10g of the above mixed powder was contained in every 100g of the mixed solution) using deionized water as the aqueous phase; 50g of the oil phase and 50g of the aqueous phase were mixed and stirred at 3000r / min for 10 minutes to obtain the water-oil two-phase repair and moisturizing active composition of Example 4.

[0032] Example 5: Preparation of Phellodendron amurense fat-soluble extract: 10g of Phellodendron amurense bark powder was mixed evenly with 30g of fermented red pine nut oil prepared in Example 2. The mixture was heated to 40℃ and stirred for 3 hours at a stirring speed of 200r / min. The mixture was then cooled to room temperature, the insoluble matter was filtered off, and the oil phase filtrate was collected to obtain the Phellodendron amurense fat-soluble extract.

[0033] Preparation of water-soluble mulberry leaf extract: Mix 10g of mulberry leaf powder with 70g of deionized water until homogeneous, heat to 60℃, and stir for 2 hours at a stirring speed of 200r / min. Then cool to room temperature, filter out insoluble matter, collect the aqueous filtrate, and freeze-dry to constant weight in a freeze dryer to obtain the water-soluble mulberry leaf extract.

[0034] Preparation of water-soluble extract of Artemisia argyi: Mix 10g of Artemisia argyi powder with 70g of deionized water until homogeneous, heat to 60℃, and stir for 2 hours at a stirring speed of 200r / min. Then cool to room temperature, filter out the insoluble matter, collect the aqueous filtrate, and freeze-dry it in a freeze dryer until constant weight to obtain water-soluble extract of Artemisia argyi.

[0035] Preparation of the water-oil two-phase composition: The above-prepared pine bark fat-soluble extract was used as the oil phase; the mulberry leaf water-soluble extract and the artemisia argyi water-soluble extract were mixed evenly at a mass ratio of 1:8 to obtain a mixed powder, and the mixed powder was prepared into a 10wt% mixed solution using deionized water as the aqueous phase; 66.7g of the oil phase and 33.3g of the aqueous phase were mixed and stirred at 3000r / min for 10 minutes to obtain the water-oil two-phase repair and moisturizing active composition of Example 5.

[0036] Example 6: Preparation of Phellodendron amurense fat-soluble extract: 10g of Phellodendron amurense bark powder was mixed evenly with 50g of fermented red pine nut oil prepared in Example 3. The mixture was heated to 50℃ and stirred for 5 hours at a stirring speed of 200r / min. The mixture was then cooled to room temperature, the insoluble matter was filtered off, and the oil phase filtrate was collected to obtain the Phellodendron amurense fat-soluble extract.

[0037] Preparation of water-soluble mulberry leaf extract: Mix 10g of mulberry leaf powder with 90g of deionized water until homogeneous, heat to 70℃, and stir for 4 hours at a stirring speed of 200r / min. Then cool to room temperature, filter out insoluble matter, collect the aqueous filtrate, and freeze-dry to constant weight in a freeze dryer to obtain the water-soluble mulberry leaf extract.

[0038] Preparation of water-soluble extract of Artemisia argyi: Mix 10g of Artemisia argyi powder with 90g of deionized water until homogeneous, heat to 70℃, and stir for 4 hours at a stirring speed of 200r / min. Then cool to room temperature, filter out the insoluble matter, collect the aqueous filtrate, and freeze-dry it in a freeze dryer until constant weight to obtain water-soluble extract of Artemisia argyi.

[0039] Preparation of the water-oil two-phase composition: The above-prepared turmeric fat-soluble extract was used as the oil phase; the mulberry leaf water-soluble extract and the artemisia argyi water-soluble extract were mixed evenly at a mass ratio of 1:12 to obtain a mixed powder, and the mixed powder was prepared into a 10wt% mixed solution using deionized water as the aqueous phase; 33.3g of the oil phase and 66.7g of the aqueous phase were mixed and stirred at 3000r / min for 10 minutes to obtain the water-oil two-phase repair and moisturizing active composition of Example 6.

[0040] Comparative Examples 4-9: Preparation of Control Compositions Comparative Example 4: The oil phase was only the fermented red pine nut oil prepared in Example 1, without the addition of Phellodendron amurense fat-soluble extract; the preparation methods of mulberry leaf water-soluble extract and Artemisia argyi water-soluble extract were exactly the same as in Example 4; the preparation method of the aqueous phase was exactly the same as in Example 4; 50g of fermented red pine nut oil was taken as the oil phase and mixed with 50g of aqueous phase at 3000r / min for 10 minutes to obtain the control composition of Comparative Example 4.

[0041] Comparative Example 5: No water-soluble mulberry leaf extract was added; only water-soluble Artemisia argyi extract was used to prepare the aqueous phase. The preparation method of Phellodendron amurense fat-soluble extract was exactly the same as in Example 4. The preparation method of Artemisia argyi water-soluble extract was exactly the same as in Example 4. Aqueous phase preparation: Artemisia argyi water-soluble extract was prepared into a 10wt% solution using deionized water as the aqueous phase. 50g of Phellodendron amurense fat-soluble extract was used as the oil phase and mixed with 50g of the aqueous phase at 3000r / min for 10 minutes to obtain the control composition of Comparative Example 5.

[0042] Comparative Example 6: No Artemisia argyi water-soluble extract was added; only Mulberry leaf water-soluble extract was used to prepare the aqueous phase. The preparation method of Phellodendron amurense fat-soluble extract was exactly the same as in Example 4. The preparation method of Mulberry leaf water-soluble extract was exactly the same as in Example 4. Aqueous phase preparation: Mulberry leaf water-soluble extract was prepared into a 10wt% solution using deionized water as the aqueous phase. 50g of Phellodendron amurense fat-soluble extract was used as the oil phase and mixed with 50g of the aqueous phase at 3000r / min for 10 minutes to obtain the control composition of Comparative Example 6.

[0043] Comparative Example 7: The fat-soluble extract of Phellodendron bark was prepared by replacing the fermented Phellodendron bark oil of Example 1 with the ordinary pressed red pine seed oil of Comparative Example 1. The rest of the preparation process and parameters were exactly the same as those of Example 4, and the control composition of Comparative Example 7 was obtained.

[0044] Comparative Example 8: Fermented red pine nut oil from Comparative Example 2 was used instead of fermented red pine nut oil from Example 1 to prepare a fat-soluble extract of Phellodendron amurense. The remaining preparation processes and parameters were exactly the same as in Example 4, resulting in the control composition of Comparative Example 8.

[0045] Comparative Example 9: Fermented red pine nut oil of Comparative Example 3 was used instead of fermented red pine nut oil of Example 1 to prepare a fat-soluble extract of Phellodendron amurense. The rest of the preparation process and parameters were exactly the same as in Example 4, and the control composition of Comparative Example 9 was obtained.

[0046] Comparative Example A: Only the oil phase was retained, and the aqueous phase was not prepared; the preparation method of the fat-soluble extract of Phellodendron amurense was exactly the same as in Example 4; the fat-soluble extract of Phellodendron amurense was directly used as Comparative Example A.

[0047] Comparative Example B: Only the aqueous phase was retained, and the oil phase was not prepared; the preparation methods of the mulberry leaf water-soluble extract and the artemisia argyi water-soluble extract were exactly the same as in Example 4; the preparation method of the aqueous phase was exactly the same as in Example 4; the aqueous phase was directly used as Comparative Example B.

[0048] Safety test In accordance with the 2015 Cosmetic Safety Technical Specifications, skin patch tests were conducted on the water-oil two-phase repairing and moisturizing active compositions of Examples 4-6. Thirty healthy volunteers aged 18-60 years with no history of skin diseases and who had not used hormonal drugs or anti-allergy drugs within the past month were selected.

[0049] Test Method: 0.020-0.025g of the test substance was placed in a patch applicator, which was then attached to the back of the subject using non-irritating cloth tape for 24 hours. Each subject was included in all embodiments. Skin reactions were observed at 30 minutes, 24 hours, and 48 hours after removing the patch applicator and evaluated according to the following criteria: Grade 0 was a negative reaction with no reaction; Grade 1 was a questionable reaction with only slight erythema; Grade 2 was a weak positive reaction with erythema, infiltration, edema, and possible papules; Grade 3 was a strong positive reaction with erythema, infiltration, edema, and papules extending beyond the tested area; Grade 4 was an extremely strong positive reaction with significant erythema, severe infiltration, edema, and confluent herpes, extending beyond the tested area.

[0050] Test results: All subjects showed a negative skin reaction of grade 0 at all three observation time points, indicating that the water-oil two-phase repair and moisturizing active composition provided by the present invention is gentle and non-irritating to the skin and safe to use.

[0051] Efficacy verification test Experiment 1: Zebrafish Tail Moisturizing Test Healthy zebrafish juveniles with a 2dpf were selected and randomly divided into a blank control group, a model group, a positive control group (0.05% urea), and a sample group, with 8 fish in each group.

[0052] Test method: Zebrafish in the sample group were placed in a culture medium containing 0.1% (w / v) of the test sample (Examples 4-6, Comparative Examples 4-9, Comparative Example A, Comparative Example B; before use, the test sample was sonicated for 5 minutes at 200W power and 25℃ constant temperature until no water-oil phase droplets larger than 1μm were visible under an optical microscope, and no stratification was observed after standing for 1 hour). The positive control group was placed in a culture medium containing 0.05% urea, and the blank control group and model group were placed in ordinary culture medium. They were incubated at 28℃ in the dark for 24 hours. Subsequently, the zebrafish in the model group, positive control group, and sample group were transferred to a high osmotic pressure solution (800mOsm / L mannitol), while the blank control group remained in ordinary culture medium. After 1 hour, the tail area of ​​the zebrafish was observed and measured, and the tail area reduction inhibition rate was calculated.

[0053] Calculation formula: Tail area reduction inhibition rate (%) = (Sample group tail area - Model group tail area) / (Blank group tail area - Model group tail area) × 100% Relative tail area reduction inhibition rate: The tail area reduction inhibition rate of the positive control group was 100%, and the relative inhibition rate of the other groups was calculated proportionally.

[0054] The test results are shown in Table 1 below: Table 1. Results of the zebrafish tail moisturizing test (relative tail area reduction inhibition rate %) Positive control group 100.0 Example 4 92.5 Example 5 88.7 Example 6 86.2 Comparative Example 4 75.2 Comparative Example 5 63.7 Comparative Example 6 65.1 Comparative Example 7 71.8 Comparative Example 8 73.2 Comparative Example 9 68.9 Comparative Example A 58.2 Comparative Example B 55.6 Data analysis: The relative tail area reduction inhibition rates of Examples 4-6 were all above 88%, significantly higher than all comparative examples. Compared with Example 4, Comparative Example 4 lacked the fat-soluble extract of Phellodendron amurense, resulting in a 17.3 percentage point decrease in inhibition rate; Comparative Examples 5-6 lacked the water-soluble extracts of mulberry leaf or Artemisia argyi, resulting in a decrease in inhibition rate of over 27.4 percentage points; Comparative Examples 7-9 used commercially available cold-pressed red pine nut oil, Trichoderma viride-fermented red pine nut oil, and Saccharomyces cerevisiae-fermented red pine nut oil, respectively, resulting in a decrease in inhibition rate of over 19.3 percentage points; Comparative Examples AB were single-phase components, with an inhibition rate of less than 59%. The above results demonstrate that the raw materials of this invention have a significant synergistic effect in terms of moisturizing efficacy.

[0055] Experiment 2: Skin Barrier Repair Test (TEWL Test) A human skin barrier damage model was established using the tape peeling method to evaluate the repair efficacy of the samples. Thirty healthy volunteers were selected, and a 3cm × 3cm test area was marked on the flexor side of the forearm. Tape was repeatedly applied and peeled off until slight erythema appeared on the skin. At this point, the TEWL value increased by more than 50% compared with the baseline value, indicating that the barrier damage model was successfully established.

[0056] Test Method: Samples from Examples 4-6, Comparative Examples 4-9, Comparative Example A, and Comparative Example B (before use, the test samples were ultrasonically treated for 5 minutes at 200W power and 25℃ until no water-oil phase droplets larger than 1μm were visible under an optical microscope, and no stratification was observed after standing for 1 hour) were uniformly applied to each test area, with an application amount of 2mg / cm² per area. Transdermal water loss (TEWL) values ​​were measured using a transdermal moisture loss meter 48 hours after application, and the 48-hour barrier repair rate was calculated.

[0057] Calculation formula: Barrier repair rate (%) = (Initial damaged TEWL value - 48h TEWL value) / (Initial damaged TEWL value - Baseline TEWL value) × 100% The test results are shown in Table 2 below: Table 2 Results of the skin barrier repair test (barrier repair rate % at 48h) Example 4 77.3 Example 5 75.5 Example 6 75.1 Comparative Example 4 53.5 Comparative Example 5 41.7 Comparative Example 6 38.2 Comparative Example 7 48.6 Comparative Example 8 54.6 Comparative Example 9 43.1 Comparative Example A 35.8 Comparative Example B 32.4 Data analysis: The 48-hour barrier repair rates of Examples 4-6 were all above 75%, significantly higher than all comparative examples. Comparative Example 4 lacked Phellodendron amurense extract, resulting in a 23.8 percentage point decrease in repair rate; Comparative Examples 5-6 lacked mulberry leaf or Artemisia argyi extracts, respectively, resulting in a more than 35.6 percentage point decrease in repair rate; Comparative Examples 7-9 used commercially available cold-pressed red pine nut oil, Trichoderma viride-fermented red pine nut oil, and Saccharomyces cerevisiae-fermented red pine nut oil, respectively, resulting in a more than 22.7 percentage point decrease in repair rate; Comparative Examples AB were single-phase components, with a repair rate of less than 36%. These results further demonstrate the significant synergistic effect of the various raw materials in this invention on skin barrier repair.

[0058] Experiment 3: ABTS free radical scavenging test Mix 5 mL of 7 mmol / L ABTS solution and 88 μL of 140 mmol / L potassium persulfate solution thoroughly to prepare ABTS radical stock solution. Let it stand at room temperature in the dark for 12 hours before use. Dilute the ABTS radical stock solution with anhydrous ethanol until the absorbance at 734 nm is 0.70 ± 0.02 to obtain the ABTS radical working solution.

[0059] Take 0.5 mL of the test sample (Examples 4-6, Comparative Examples 4-9, Comparative Example A, Comparative Example B) solution (before use, the test sample was sonicated for 5 minutes at 200W power and 25℃ until no water-oil phase droplets larger than 1 μm were visible under an optical microscope, and no stratification was observed after standing for 1 hour), add 3.5 mL of ABTS free radical working solution, shake for 30 s, and let stand at room temperature in the dark for 6 min. Use anhydrous ethanol as a blank control and measure the absorbance at 734 nm. Each sample was measured three times, and the average value was taken. Vitamin C (Vc, 50 μg / mL) was used as a positive control.

[0060] Calculation formula: ABTS free radical scavenging rate (%) = (A0 - A) i +A j ) / A0×100% Where A0 is the absorbance of the blank control without the test solution; A i A represents the absorbance after adding the test solution; j The background absorbance of the test liquid is denoted as .

[0061] The test results are shown in Table 3 below: Table 3 Results of ABTS free radical scavenging test (scavenging rate %) Positive control group (Vc) 98.7 Example 4 69.2 Example 5 62.6 Example 6 67.4 Comparative Example 4 42.3 Comparative Example 5 40.7 Comparative Example 6 37.1 Comparative Example 7 47.8 Comparative Example 8 40.4 Comparative Example 9 44.9 Comparative Example A 28.6 Comparative Example B 32.9 Data analysis: The ABTS free radical scavenging rates of Examples 4-6 were all above 62%, significantly higher than all comparative examples. Comparative example 4 lacked Phellodendron amurense extract, resulting in a 26.9 percentage point decrease in scavenging rate; Comparative examples 5-6 lacked mulberry leaf or Artemisia argyi extracts, respectively, resulting in a decrease in scavenging rate of over 28.5 percentage points; Comparative examples 7-9 used commercially available cold-pressed red pine nut oil, Trichoderma viride-fermented red pine nut oil, and Saccharomyces cerevisiae-fermented red pine nut oil, respectively, resulting in a decrease in scavenging rate of over 21 percentage points; Comparative example AB was a single-phase component, with a scavenging rate of less than 55%. These results indicate that the compositions of the present invention possess excellent antioxidant effects, and the various raw materials also exhibit significant synergistic effects in antioxidant properties.

[0062] Test 4: Stability Test Preparation of water-oil two-phase body oil Body oil 1: Composed of the following components by mass percentage: Example 4: 50 wt% Deionized water: 22.5 wt% Complex base oils: 22.5 wt%; The composite base oil is composed of jojoba seed oil, camellia seed oil, and sunflower seed oil in a ratio of 1:0.7:0.5.

[0063] Body oil 1 was stored at 4℃, 25℃, and 45℃ for 3 months, and its appearance, layering, and pH value were observed monthly.

[0064] Test results: After being stored for 3 months under three different temperature conditions, body oil 1 maintained a clear water-oil two-phase separation, with no turbidity, no sedimentation, no discoloration, and no oil-water miscibility; it could be restored to a uniform dispersion state within 10 seconds after shaking; after shaking, the pH value was stable between 5.5 and 6.5, which meets the stability requirements of cosmetic products.

[0065] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A water-oil two-phase active composition, characterized in that, Including active ingredients such as Phellodendron bark extract, mulberry leaf extract, and Artemisia argyi extract; Among them, the Phellodendron amurense extract is a fat-soluble extract of Phellodendron amurense, which is prepared by extraction using fermented red pine seed oil as a solvent; Among them, the mulberry leaf extract is a water-soluble extract of mulberry leaves, which is prepared by extraction with deionized water as a solvent; The Artemisia argyi extract is a water-soluble extract of Artemisia argyi, prepared by extraction with deionized water as a solvent. The fermented red pine nut oil mentioned above was prepared by fermentation using red pine nut powder as raw material and Trichoderma reesei as the strain.

2. The composition according to claim 1, characterized in that, The method for preparing fermented red pine nut oil includes the following steps: S1: Take dried red pine nuts, grind them into powder, and then soak them in water for 12-16 hours to obtain suspension A. S2: Take suspension A, sterilize it, and let it cool to room temperature. Inoculate it with Trichoderma reesei culture and ferment for 24-48 hours. The fermentation start temperature is 28-30℃. Then, inactivate and filter it, separate the liquids, and take the oil to obtain fermented red pine nut oil.

3. The composition according to claim 2, characterized in that, The inoculum size of *Trichoderma reesei* was 1-3 wt% of the suspension; the viable count in the *Trichoderma reesei* inoculum was 1 × 10⁻⁶. 6 -1×10 8 CFU / mL.

4. The composition according to claim 1, characterized in that, The method for preparing the composition includes the following steps: Preparation of Phellodendron bark extract: Phellodendron bark powder and fermented red pine nut oil were mixed evenly at a mass ratio of 1:3-5, heated to 40-50℃, extracted for 3-5 hours, then cooled to room temperature, filtered, and the oil phase was collected to obtain Phellodendron extract. Preparation of mulberry leaf extract: Mulberry leaf powder and deionized water were mixed evenly at a mass ratio of 1:7-9, heated to 60-70℃, extracted for 2-4 hours, then cooled to room temperature, filtered to obtain the aqueous phase, and freeze-dried to obtain mulberry leaf extract. Preparation of Artemisia argyi extract: Artemisia argyi powder and deionized water were mixed evenly at a mass ratio of 1:7-9, heated to 60-70℃, extracted for 2-4 hours, then cooled to room temperature, filtered to obtain the aqueous phase, and freeze-dried to obtain Artemisia argyi extract. Preparation of water-oil two-phase active composition: using Phellodendron amurense extract as the oil phase; Mulberry leaf extract and artemisia extract were mixed evenly at a mass ratio of 1:(8-12) to obtain a mixed powder. The mixed powder was then prepared into a 10wt% mixed solution using deionized water to obtain an aqueous phase. The oil phase and the water phase are mixed at a mass ratio of 1:(0.5-2) to obtain an active water-oil two-phase composition.

5. The use of the composition according to any one of claims 1-4 in the preparation of a skin care product having soothing and / or repairing and / or moisturizing and / or antioxidant effects; wherein the skin care product dosage form includes any one of lotion, serum, cream, mask, spray, bath oil, and body oil.

6. A body oil, characterized in that, The composition comprises 1-99 wt% of any one of claims 1-4.

7. The body oil as described in claim 6, characterized in that, It also includes one or more of the following: base oil, emulsifier, moisturizer, and deionized water.

8. The body oil as described in claim 6, characterized in that, The base oils include jojoba seed oil and / or camellia seed oil and / or sunflower seed oil.

9. The body oil as described in claim 6, characterized in that, The emulsifiers include lauryl ether-4 and / or PEG-25 hydrogenated castor oil.

10. The body oil as described in claim 6, characterized in that, The moisturizers include sodium hyaluronate, propylene glycol, and / or glycerin.