A traditional Chinese medicine composition with antioxidant and acetaldehyde dehydrogenase activating efficacy and a preparation method thereof

Abstract

This invention discloses a traditional Chinese medicine composition with antioxidant and acetaldehyde dehydrogenase activating effects, and its preparation method. The composition comprises, by weight, 9-12 parts lily bulb, 6-9 parts turmeric, 9-12 parts fenugreek, and 9-12 parts schisandra chinensis. This invention addresses the human body's need for antioxidant activity and acetaldehyde dehydrogenase activation to accelerate acetaldehyde metabolism. It utilizes a combination of natural products, eliminating the need for synthesis and structural modification, thus reducing side effects in the human body and exhibiting good antioxidant and acetaldehyde dehydrogenase activating effects.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine composition technology, specifically relating to a traditional Chinese medicine composition with antioxidant and acetaldehyde dehydrogenase activating effects and its preparation method. Background Technology

[0002] Antioxidant refers to the biological process by which biomolecules such as DNA, proteins, and lipids are protected from oxidative damage by neutralizing free radicals or blocking oxidation chain reactions. The human body produces free radicals during normal metabolism, but when they accumulate in excess, they trigger oxidative stress, leading to cellular aging, inflammation, and various chronic diseases.

[0003] The core principle of acetaldehyde dehydrogenase (ALDH) activation lies in regulating the enzyme's conformation or microenvironment through specific substances, thereby enhancing its efficiency in catalyzing the conversion of acetaldehyde to acetic acid and reducing the damage of acetaldehyde to the human body.

[0004] Currently, existing technologies for antioxidant and acetaldehyde dehydrogenase activation mainly face problems such as low bioavailability, poor stability, and difficulty in optimizing synergistic effects. Summary of the Invention The present invention aims to overcome the shortcomings of the prior art and provide a traditional Chinese medicine composition with antioxidant and acetaldehyde dehydrogenase activating effects. This traditional Chinese medicine composition has the characteristics of simple process, convenient operation and strong effect.

[0005] As a first aspect of the present invention, the present invention provides a traditional Chinese medicine composition with antioxidant and acetaldehyde dehydrogenase activating effects, wherein the raw materials are composed of 9-12 parts of lily bulb, 6-9 parts of turmeric, 9-12 parts of fenugreek, and 9-12 parts of schisandra chinensis by weight; the traditional Chinese medicine composition is obtained by raw material extraction, concentration, purification, and drying.

[0006] In this invention, lily refers to the dried, fleshy scales of plants belonging to the genus *Lilium lancifolium*, *L. brownii* var. *viridulum*, or *L. pumilum* in the family Liliaceae. It is sweet and cold in nature, and enters the heart and lung meridians. Its polysaccharides and phenolic components can scavenge free radicals and enhance macrophage activity, potentially offering benefits for chronic fatigue and oxidative stress-related diseases.

[0007] Turmeric is the dried rhizome of the ginger family plant Curcuma longa L. It is pungent, bitter, and warm in nature, and enters the spleen and liver meridians. Its active ingredient curcumin has modern pharmacological effects such as anti-inflammatory, antioxidant, and liver-protective properties.

[0008] Fenugreek is the dried, mature seed of the legume Trigonella foenum-graecum L. It is bitter and warm in nature, and enters the kidney meridian. Its active ingredient, trigonelline, has antioxidant, blood sugar regulating, anti-inflammatory, and liver-protective effects. Schisandra chinensis is the dried, ripe fruit of the Schisandra chinensis plant (commonly known as "Northern Schisandra"). It is sour, sweet, and warm in nature, and enters the lung, heart, and kidney meridians. Its active ingredients, lignans (such as schisandrol A), have clear liver-protecting and antioxidant effects.

[0009] In a preferred embodiment, the traditional Chinese medicine composition comprises, by weight, 12 parts lily bulb, 9 parts turmeric, 9 parts fenugreek, and 9 parts schisandra chinensis.

[0010] As a second aspect of the present invention, the present invention provides a method for preparing the above-mentioned traditional Chinese medicine composition, comprising the following steps: S1 Extraction: Weigh the raw materials according to the following weight proportions: 9-12 parts of lily bulb, 6-9 parts of turmeric, 9-12 parts of fenugreek, and 9-12 parts of schisandra chinensis. Then, pulverize them and extract them with ultrasonic ethanol solution. After extraction, cool to room temperature, filter, and combine the filtrates. S2 Concentration and Drying: The filtered extract is concentrated and dried under reduced pressure and vacuum. S3 Preparation and purification: Dissolve the above extract in 5 times 35% ethanol, adsorb onto an AB-8 resin column for 1 hour, elute with 5 times ultrapure water for 0.5 hours, elute with 5 times 35% ethanol for 1 hour, and collect the eluent. S4 Concentration and Drying: The collected 35% ethanol and ultrapure water eluent is concentrated and dried under reduced pressure and vacuum to obtain the final product.

[0011] In a preferred embodiment, 10 times the volume of 35% ethanol solution is added to S1, and ultrasonic extraction is performed 2-3 times at 450-500W, each time for 80-90 minutes.

[0012] In a preferred embodiment, the drying temperature in S2 and S4 is 45-50°C.

[0013] As a third aspect of the present invention, the present invention provides the application of the above-mentioned traditional Chinese medicine composition in the preparation of products with antioxidant and acetaldehyde dehydrogenase activating effects.

[0014] Compared with the prior art, the present invention has the following advantages: 1. Simple process and minimal side effects: Compared to other antioxidant and aldehyde dehydrogenase activator products, this combination uses only natural products, requiring no synthesis or structural modification, thus reducing the risk of side effects. It also eliminates the need for additional auxiliary drugs, maintaining the pure natural source of the ingredients.

[0015] 2. High promotion rate: Compared with other products with antioxidant and aldehyde dehydrogenase activating effects, the traditional Chinese medicine composition of this invention has been found to have better antioxidant and aldehyde dehydrogenase activating effects through high-throughput screening experiments of oxygen free radicals and aldehyde dehydrogenase, with significant effects.

[0016] 3. The composition of the present invention is a functional combination based on modern pharmacological mechanisms, with a compatibility mode mainly based on multi-target synergistic effect, and has the characteristics of multi-target synergistic effect. Detailed Implementation

[0017] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are for illustrative purposes only and are not intended to limit the invention in any way. It should be noted that, unless otherwise specified, the following embodiments and features can be combined with each other. It should also be understood that the terminology used in the embodiments of this invention is for describing specific implementation schemes and not for limiting the scope of protection of this invention.

[0018] When a range of values ​​is given, it should be understood that, unless otherwise stated in this invention, the two endpoints of each range and any value between the two endpoints may be selected.

[0019] Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly used in the field to which this invention pertains. The terminology used to describe this invention is intended only to describe a particular implementation and is not intended to limit the scope of the teachings. This invention can be implemented using any prior art methods, apparatus, and materials similar to or equivalent to those described, used, or made in the embodiments of this invention.

[0020] The term “and / or” as used herein should be understood to mean any one of the options or any combination of two or more of the options.

[0021] As used herein, the term "about" indicates a range of ±20% of the following value. In some embodiments, the term "about" indicates a range of ±10% of the following value. In some embodiments, the term "about" indicates a range of ±5% of the following value.

[0022] The following embodiments are provided to aid in understanding the present invention. However, it should be understood that these embodiments are for illustrative purposes only and do not constitute any limitation. The actual scope of protection of the present invention is set forth in the claims. It should be understood that any modifications and changes can be made without departing from the spirit of the present invention. Unless otherwise specified, the raw materials used in the embodiments are all commercially available products in the art, their specifications are conventional specifications in the art, and the methods used are conventional methods in the art. The instruments and equipment used in the embodiments are conventional instruments and equipment in the art.

[0023] In this specific embodiment, the traditional Chinese medicine compositions prepared in the examples and comparative examples were subjected to high-throughput screening based on ORAC antioxidant and acetaldehyde dehydrogenase. The model specifically includes: Based on the ORAC antioxidant high-throughput model: The ORAC reaction is a classic hydrogen atom transfer oxidation process. Under experimental conditions, one molecule of AAPH loses one molecule of nitrogen gas, generating two molecules of AAPH radicals. In air, the generated AAPH radicals quickly react with O2 to form relatively stable peroxy radicals ROO·. The fluorescence decay curve of fluorescein FL indicates the degree of damage to fluorescein by peroxy radicals. In the absence of antioxidants, ROO· gains a hydrogen atom from FL, causing fluorescence decay of fluorescein; in the presence of antioxidants in the sample, ROO· gains a hydrogen atom from the antioxidant, generating ROOH and a stable antioxidant radical, thus inhibiting the rate of fluorescein degradation by peroxy radicals. The ORAC value of the sample is obtained by comparing the net area under the fluorescence decay curve of the sample with the net area under the curve of the standard antioxidant Trolox.

[0024] Based on the high-throughput model of aldehyde dehydrogenase: aldehyde dehydrogenase (ALDH), NAD+ and substrate react under certain temperature and pH conditions. By measuring the change in absorbance at a wavelength of 340 nm per minute, the amount of NAD+ converted to NADH per minute can be calculated. The activation rate of the tested sample is obtained by comparing the sample group with the blank group.

[0025] The solution preparation method is as follows: ORAC antioxidant model: (1) Buffer solution stock solution 0.75 M K₂HPO₄: 130 g K₂HPO₄ dissolved in 1 L of water; 0.75 M NaH2PO4: 90 g NaH2PO4 dissolved in 1 L of water.

[0026] (2) Buffer solution working solution Take 81 mL of K2HPO4 stock solution and 19 mL of NaH2PO4 stock solution respectively, mix them, and dilute to 1000 mL with water to obtain a 75 mM buffer solution with pH 7.4. Store in a refrigerator.

[0027] (3) Sodium fluorescein solution Fluorescein sodium stock solution 1: Weigh 0.0456 g of fluorescein sodium salt and bring the volume to 100 mL of phosphate buffer. Store in the dark. Fluorescein sodium stock solution 2: Bring 1000 μL of stock solution 1 to a final volume of 100 mL with phosphate buffer. Store in the dark. Fluorescein sodium working solution: Bring 1000 μL of stock solution 2 to a final volume of 100 mL with phosphate buffer. Store in the dark.

[0028] (4) Trolox standard solution 0.0125 g Trolox was diluted to 50 mL of phosphate buffer to obtain a 1000 μM stock solution, which was stored at -20 °C. Then, it was successively diluted with phosphate buffer to prepare working solutions of 300 μM, 200 μM, 100 μM, 50 μM, 25 μM, and 12.5 μM.

[0029] (5) AAPH solution Dissolve 0.414g of AAPH completely in 10mL of 75mM phosphate buffer solution (pH 7.4) to obtain a 153mM solution. Store on ice. (Prepare fresh before use.) acetaldehyde dehydrogenase model: (1) Buffer Dissolve 0.8 g of potassium dihydrogen phosphate and 21.4 g of dipotassium hydrogen phosphate in 800 mL of water, adjust the pH to 8.0, and bring the volume to 1000 mL to prepare a 100 mmol / L solution. Sterilize and store at 4°C. Note: Ultrapure water was used.

[0030] (2) ALDH solution ALDH stock solution: Prepare a 2 U / mL solution using a buffer solution, dispense into 1.2 mL EP tubes, and store at -20°C.

[0031] ALDH working solution: Before the experiment, take the ALDH stock solution, thaw it at room temperature, and dilute it to 1U / mL for use in the experiment.

[0032] (3)NAD+ solution (MW=663.43) NAD+ stock solution (8 mmol / L): Accurately weigh 132.68 mg of NAD+ powder and dilute to 25 mL with buffer solution. Aliquot into 1.0 mL EP tubes and store at -20 °C.

[0033] NAD+ working solution (2 mmol / L): Dissolve NAD+ stock solution at room temperature and bring the volume up to 4 mL. Use the solution for experiments.

[0034] (4)DTT solution DTT stock solution (4 mmol / L): Accurately weigh 6.16 mg of DTT powder (MW=154.25) and dilute to 10 mL. Store at 4°C (shelf life 7 days).

[0035] DTT working solution (0.4 mmol / L): Take 1 mL of DTT stock solution and dilute to 10 mL for use in experiments.

[0036] (5) Acetaldehyde solution (0.05 mmol / L): Take 2.564 mL of 40% acetaldehyde solution, make up to 100 mL, and store at 4℃ in a refrigerator.

[0037] Experimental Operations and Calculations ORAC antioxidant model: Blank BLANK, sample, and standard antioxidant (Trolox) were mixed with fluorescein solution and incubated at 37°C for 30 min in an ELISA reader. Then, AAPH solution was quickly added, and the measurement was started in the ELISA reader. The fluorescence intensity was recorded using the software provided with the ELISA reader. The initial fluorescence intensity value was recorded as f0, and measurements were taken every two minutes thereafter for 120 minutes. The fluorescence intensities were recorded as f0, f1, f2, ... f61.

[0038] The relevant parameters of the instrument and method are as follows: excitation wavelength 485±20nm, emission wavelength 528±20nm, automatic sensitivity, halogen lamp as the light source. Blank, sample, standard antioxidant (Trolox) 20μL, fluorescein sodium solution 160μL, AAPH solution 20μL.

[0039] The area under the fluorescence decay curve (AUC) is calculated using the formula: AUC = f0 / f0 + 2 × (f1 / f0 + ... + f60 / f0) + f61 / f0. This is the integrated area under the fluorescence decay curve with the presence of an antioxidant. Subtracting the area under the blank curve without an antioxidant yields the net AUC. A standard curve is plotted with the Trolox standard solution concentration on the x-axis and the corresponding net AUC on the y-axis. A regression equation is derived, and the Net AUC generated by the sample is substituted into the equation to determine the concentration.

[0040] acetaldehyde dehydrogenase model: Instrument and method parameters: Wavelength: 340nm; Absorbance error: ±0.0001; Light source: Halogen lamp Reaction system volumes: Reagent buffer or test solution: 30 μL; Buffer solution: 75 μL; Dithiothreitol: 40 μL; Acetaldehyde dehydrogenase: 30 μL; NAD+ solution: 30 μL Detailed operating steps Add the following to the well plate: 75 μL of 100 mmol / L potassium phosphate buffer (pH 8.0), 40 μL of 0.4 mmol / L dithiothreitol (DTT), 30 μL of 1 U / mL acetaldehyde dehydrogenase, 30 μL of the test solution (or buffer solution), and 30 μL of 0.05 mol / L acetaldehyde solution. Incubate at 37°C for 30 min in a microplate reader. Then, quickly add 30 μL of 2.0 mmol / L NAD+ solution, shake well, and immediately begin the assay. Record the absorbance using the microplate reader's software. The initial absorbance value is recorded as f0. Measurements are taken every minute for 120 minutes, and the absorbance values ​​are recorded as f1, f2, ..., f120. The reaction ends when fx reaches its maximum value and stabilizes. The reaction time is t_max. The Δabsorbance value is calculated as fn - f0 (n = 1, 2, 3… 120). Plot a standard curve with the NAD+ standard solution concentration on the x-axis and the corresponding Δ absorbance value on the y-axis, and calculate the precision.

[0041] The technical solution of the present invention will be described in detail below through specific embodiments, but the scope of protection of the present invention is not limited thereto.

[0042] Example 1 This embodiment provides a method for preparing a traditional Chinese medicine composition with antioxidant and acetaldehyde dehydrogenase activating effects, comprising the following steps: (1) Extraction: The following components by weight were pulverized: 9 parts lily bulb, 9 parts turmeric, 12 parts fenugreek, and 12 parts schisandra chinensis. 10 times the volume of 35% ethanol solution was added, and the mixture was ultrasonically extracted three times at 500W for 85 minutes each time. After extraction, the mixture was cooled to room temperature, filtered, and the filtrates were combined. Concentration and drying: The collected fraction was concentrated and dried under reduced pressure at 45℃. (2) Preparation and purification: Dissolve the above extract in 5 times 35% ethanol, adsorb on AB-8 resin column for 1 hour, elute with 5 times ultrapure water for 0.5 hours, elute with 5 times 35% ethanol for 1 hour, and collect the eluent. (3) Concentration and drying: The collected 35% ethanol and ultrapure water eluent is concentrated and dried under reduced pressure at a temperature of 45°C to obtain the product.

[0043] (4) ORAC antioxidant and acetaldehyde dehydrogenase model detection: The prepared sample was dissolved in 5 times 35% ethanol and tested. The specific operation and calculation were as described above. The ORAC antioxidant capacity of the composition was 1620.347±58.645 μmolTE / L and the acetaldehyde dehydrogenase activation rate was 23.45%.

[0044] Example 2 This embodiment provides a method for preparing a traditional Chinese medicine composition with antioxidant and acetaldehyde dehydrogenase activating effects, comprising the following steps: (1) Extraction: The following components by weight were 12 parts lily bulb, 9 parts turmeric, 9 parts fenugreek, and 9 parts schisandra chinensis. The mixture was pulverized, and 10 times the volume of 35% ethanol solution was added. The mixture was ultrasonically extracted three times at 450W for 90 minutes each time. After extraction, the mixture was cooled to room temperature, filtered, and the filtrates were combined. Concentration and drying: The collected fraction was concentrated and dried under reduced pressure at 45℃. (2) Preparation and purification: Dissolve the above extract in 5 times 35% ethanol, adsorb on AB-8 resin column for 1 hour, elute with 5 times ultrapure water for 0.5 hours, elute with 5 times 35% ethanol for 1 hour, and collect the eluent.

[0045] (3) Concentration and drying: The collected 35% ethanol and ultrapure water eluent is concentrated and dried under reduced pressure at a temperature of 45°C to obtain the product.

[0046] (4) ORAC antioxidant and acetaldehyde dehydrogenase model detection: The prepared sample was dissolved in 5 times 35% ethanol and tested. The specific operation and calculation were as described above. The ORAC antioxidant capacity of the composition was 1881.33±72.5 μmolTE / L and the acetaldehyde dehydrogenase activation rate was 27.01%.

[0047] Example 3 This embodiment provides a method for preparing a traditional Chinese medicine composition with antioxidant and acetaldehyde dehydrogenase activating effects, comprising the following steps: (1) Extraction: The following components by weight were 10 parts lily bulb, 8 parts turmeric, 10 parts fenugreek, and 11 parts schisandra chinensis. These were pulverized and 10 times the volume of 35% ethanol solution was added. The mixture was ultrasonically extracted twice at 480W for 85 minutes each time. After extraction, the mixture was cooled to room temperature, filtered, and the filtrates were combined. Concentration and drying: The collected fraction was concentrated and dried under reduced pressure at 45℃. (2) Preparation and purification: Dissolve the above extract in 5 times 35% ethanol, adsorb on AB-8 resin column for 1 hour, elute with 5 times ultrapure water for 0.5 hours, elute with 5 times 35% ethanol for 1 hour, and collect the eluent. (3) Concentration and drying: The collected 35% ethanol and ultrapure water eluent is concentrated and dried under reduced pressure at a temperature of 45°C to obtain the product.

[0048] (4) ORAC antioxidant and acetaldehyde dehydrogenase model detection: The prepared sample was dissolved in 5 times 35% ethanol and tested. The specific operation and calculation were as described above. The ORAC antioxidant capacity of the composition was 1616.695±86.919 μmolTE / L and the acetaldehyde dehydrogenase activation rate was 22.10%.

[0049] Comparative Example 1 This comparative example differs from Example 1 in that it lacks the lily component, but otherwise remains the same as Example 1.

[0050] The levels of ORAC decreased from 1620.347±58.645 μmol TE / L to 657.652±94.665 μmol TE / L, and the activation rate of acetaldehyde dehydrogenase decreased from 23.45% to 8.67%. Comparative Example 2 Compared with Example 1, this comparative example lacks the turmeric component, but is otherwise the same as Example 1.

[0051] The results showed that ORAC decreased from 1620.347±58.645 μmol TE / L to 1058±25.207 μmol TE / L, and the acetaldehyde dehydrogenase activation rate decreased from 23.45% to 4.38%. Comparative Example 3 This comparative example differs from Example 1 in that it lacks the fenugreek component, but is otherwise identical to Example 1.

[0052] The levels of ORAC decreased from 1620.347±58.645 μmol TE / L to 246.875±18.134 μmol TE / L, and the acetaldehyde dehydrogenase activation rate decreased from 23.45% to 1.84%. Comparative Example 4 Compared with Example 1, this comparative example lacks the Schisandra chinensis component, but is otherwise the same as Example 1.

[0053] The levels of ORAC decreased from 1620.347±58.645 μmol TE / L to 856.618±26.856 μmol TE / L, and the acetaldehyde dehydrogenase activation rate decreased from 23.45% to 7.22%. Comparative Example 5 Compared with Example 1, this comparative example lacks the lily and turmeric components, but is otherwise the same as Example 1.

[0054] The levels of ORAC decreased from 1620.347±58.645 μmol TE / L to 152.23±14.574 μmol TE / L, and the acetaldehyde dehydrogenase activation rate decreased from 23.45% to 0.47%. Comparative Example 6 This comparative example provides a method for preparing a traditional Chinese medicine composition, comprising the following steps: (1) Extraction: Weigh out 9 portions of each of the four medicinal materials and pulverize them. Add 10 times the volume of 35% ethanol solution and extract twice by ultrasonication at 450W for 80 minutes each time. After extraction, cool to room temperature, filter, and combine the filtrates. Concentration and drying: Concentrate and dry the collected fractions under reduced pressure at 45℃. (2) Preparation and purification: Dissolve the above extract in 5 times 35% ethanol, adsorb on AB-8 resin column for 1 hour, elute with 10 times ultrapure water for 0.5 hours, elute with 5 times 35% ethanol for 1 hour, and collect the eluent.

[0055] (3) Sample preparation: After the above-collected 35% ethanol and ultrapure water eluent is concentrated and dried under vacuum, it is dissolved in 5 times the amount of 35% ethanol.

[0056] (4) Functional testing: Lily: 515.628±13.51 μmol TE / L, acetaldehyde dehydrogenase activation rate 14.05% Turmeric: 1348.330±83.452 μmol TE / L, acetaldehyde dehydrogenase activation rate 18.9%. Fenugreek: 451.034±13.68 μmol TE / L, acetaldehyde dehydrogenase activation rate 15.45%. Schisandra chinensis: 1313.134±15.998 μmol TE / L, acetaldehyde dehydrogenase activation rate 3.24%. Comparative Example 7 The only difference between this comparative example and Example 1 is the preparation and purification in step (2): the above extract was dissolved in 5 times 35% ethanol, adsorbed on an AB-8 resin column for 1 hour, eluted with 5 times ultrapure water for 0.5 hours, and the eluent was collected.

[0057] The results showed that in Comparative Example 7, the ORAC concentration decreased from 1620.347±58.645 μmol TE / L to 348.397±19.82 μmol TE / L, and the acetaldehyde dehydrogenase activation rate decreased from 23.45% to 8.57%. Comparative Example 8 The only difference between this comparative example and Example 1 is the formulation. Specifically, the proportions of each component in this comparative example are: 7 parts lily bulb, 4 parts turmeric, 14 parts fenugreek, and 14 parts schisandra.

[0058] The levels of ORAC decreased from 1620.347±58.645 μmol TE / L to 1184.538±58.645 μmol TE / L, and the activation rate of acetaldehyde dehydrogenase decreased from 23.45% to 12.54%. The above embodiments merely illustrate several implementation methods of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the patent application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of the present invention should be determined by the appended claims.

Claims

1. A traditional Chinese medicine composition with antioxidant and acetaldehyde dehydrogenase activating effects, characterized in that, The raw materials consist of 9-12 parts by weight of lily bulb, 6-9 parts of turmeric, 9-12 parts of fenugreek, and 9-12 parts of schisandra chinensis; the traditional Chinese medicine composition is prepared by raw material extraction, concentration, purification, and drying.

2. The traditional Chinese medicine composition according to claim 1, characterized in that, The ingredients consist of 12 parts lily bulbs, 9 parts turmeric, 9 parts fenugreek, and 9 parts schisandra chinensis, by weight.

3. A method for preparing the traditional Chinese medicine composition according to claim 1 or 2, characterized in that, Includes the following steps: S1 Extraction: Weigh the raw medicinal materials according to the ratio, then crush them, and use ethanol solution for ultrasonic extraction. After extraction, cool to room temperature, filter, and combine the filtrates. S2 Concentration and Drying: The filtered extract is concentrated and dried under reduced pressure and vacuum. S3 Preparation and purification: Dissolve the above extract in 5 times 35% ethanol, adsorb onto an AB-8 resin column for 1 hour, elute with 5 times ultrapure water for 0.5 hours, elute with 5 times 35% ethanol for 1 hour, and collect the eluent. S4 Concentration and Drying: The collected 35% ethanol and ultrapure water eluent is concentrated and dried under reduced pressure and vacuum to obtain the final product.

4. The preparation method according to claim 3, characterized in that, Add 10 times the volume of 35% ethanol solution to S1 and extract using ultrasound at 450-500W for 2-3 times, each time for 80-90 minutes.

5. The preparation method according to claim 3, characterized in that, The drying temperature in S2 and S4 is 45-50℃.

6. The use of the traditional Chinese medicine composition according to claim 1 or 2 in the preparation of products with antioxidant and acetaldehyde dehydrogenase activating effects.