Eye drops for improving eyesight containing selenium-containing peptide and preparation method thereof

Abstract

This invention discloses a vision-improving eye drop containing selenium peptides and its preparation method, relating to the field of eye drop technology. This selenium-containing Dendrobium eye drops, by weight, comprises the following components: 10-25 parts Dendrobium extract, 15-30 parts chrysanthemum extract, 3-8 parts pearl powder, 0.01-0.1 parts plant selenium peptide, 1-3 parts protein peptide, 1-3 parts borneol, 2-5 parts sodium hyaluronate, 0.5-2 parts lutein, 0.1-0.5 parts vitamin B6, 0.1-0.5 parts vitamin A, 0.5-2 parts taurine, and 80-120 parts water for injection. This eye drop achieves a synergistic effect of trace element anti-oxidation, small molecule peptide tissue repair, vitamin visual nutrition, and traditional Chinese medicine for clearing the liver and improving vision. This makes the eye drop more effective than traditional single or simple compound eye drops in relieving eye fatigue, improving dry eyes, and enhancing visual clarity. It also provides antioxidant protection for the lens and promotes corneal epithelial repair.

Description

Technical Field

[0001] This invention relates to the field of eye drop technology, specifically to a vision-improving eye drop containing selenium peptides and its preparation method. Background Technology

[0002] With the widespread use of electronic display devices, people spend long hours in front of computer and mobile phone screens, leading to increasingly common eye health problems such as eye strain, dry eyes, and decreased vision. These issues have become a prevalent public health problem affecting people of all ages. According to data from the National Health Commission and related epidemiological surveys, my country has a large population suffering from dry eye syndrome, and the incidence of age-related eye diseases such as refractive errors and cataracts is also high. Clinically, there is a continuously growing demand for eye care products with comprehensive conditioning functions. In the field of ophthalmic care technology, eye drops with natural plant extracts and trace elements as core active ingredients are a key solution for relieving these symptoms. As an important product form, existing technologies have reported compound eye drops containing ingredients such as dendrobium, chrysanthemum, and pearl powder. For example, the classic Pearl Brightening Eye Drops, through the combination of pearl powder and borneol, exert the basic conditioning effects of clearing heat and brightening the eyes, removing corneal opacity and relieving pain. Some other technical solutions have introduced nutrients such as taurine and vitamin A on this basis, so that the eye drops have certain nutritional support functions in addition to simple moisturizing and lubrication. The trace element selenium is an important component of glutathione peroxidase, which has been proven by studies to have antioxidant protective effects on retinal cells, iris and lens, and can delay the occurrence of cataracts and regulate tear secretion.

[0003] However, existing eye drop technologies primarily focus on traditional functions such as clearing heat and improving vision, as well as relieving superficial dryness. In practical use, these technologies have the following shortcomings: First, while existing technologies recognize the antioxidant value of selenium, they often directly use inorganic selenium, such as sodium selenite, as an additive. Inorganic selenium has limited bioavailability and uncertain metabolic conversion rates when applied to the eye, making it difficult to fully utilize its effective scavenging of free radicals on the ocular surface and its deep protection of the lens. Furthermore, improper dosage control poses a certain risk of local irritation. Moreover, existing solutions often fail to address the issue of eye strain caused by eye use. Micro-damage to the corneal surface caused by excessive dryness, environmental dryness, or minor mechanical injury lacks active biological repair capabilities, limiting the efficacy of products to passive protection. Furthermore, for mineral-based traditional Chinese medicines like pearl powder, existing processes often involve simple mechanical pulverization before incorporation, resulting in large particle sizes and low solubility. This makes it difficult for the active ingredients to be rapidly released and absorbed by eye tissues under tear flushing, affecting bioavailability and easily causing discomfort due to the granular texture. Therefore, to address the shortcomings of existing technologies, this invention provides a selenium-containing eye drop for improving eyesight and its preparation method to solve the above problems. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a vision-improving eye drop containing selenium peptides and its preparation method, thereby resolving the problems mentioned in the background section.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solution: a vision-improving eye drop containing selenium peptides, comprising the following components by weight: 10-25 parts of Dendrobium extract, 15-30 parts of chrysanthemum extract, 3-8 parts of pearl powder, 0.01-0.1 parts of plant selenium peptides, 1-3 parts of protein peptides, 1-3 parts of borneol, 2-5 parts of sodium hyaluronate, 0.5-2 parts of lutein, 0.1-0.5 parts of vitamin B6, 0.1-0.5 parts of vitamin A, 0.5-2 parts of taurine, and 80-120 parts of water for injection.

[0006] Preferably, the Dendrobium extract is an aqueous extract of Dendrobium officinale or Dendrobium nobile; the chrysanthemum extract is an aqueous extract of Chrysanthemum indicum or Chrysanthemum indicum.

[0007] Preferably, the pearl powder is an ultrafine powder treated with lactic acid reaction. The preparation method includes: crushing pearl into coarse powder, adding water for injection and food-grade lactic acid to react, filtering, concentrating, drying and then ultrafine pulverizing to obtain pearl ultrafine powder with a particle size of 300-500 mesh.

[0008] Preferably, the plant selenium peptide is a small molecule selenium peptide obtained from selenium-enriched broccoli through low-temperature compound enzymatic hydrolysis and membrane separation technology, with a molecular weight of less than 3000 Daltons.

[0009] Preferably, the protein peptide is a walnut protein peptide, which is obtained by defatting, compound enzymatic hydrolysis and ultrafiltration membrane separation technology from walnut cake meal, and its molecular weight is less than 5000 Daltons.

[0010] Preferably, the eye drops are preservative-free, sterilized by filtration through a 0.22μm microporous membrane, and have a pH value of 6.5–7.5.

[0011] This invention also discloses a method for preparing the aforementioned selenium-containing eye drops for improving eyesight, comprising the following steps:

[0012] Step S1: Weigh out the following ingredients according to the specified ratio: Dendrobium extract, Chrysanthemum extract, Pearl powder, Plant selenium peptide, Protein peptide, Borneol, Sodium hyaluronate, Lutein, Vitamin B6, Vitamin A, Taurine, and Water for Injection.

[0013] Step S2: Add pearl powder to the first part of water for injection and stir to prepare a pearl powder suspension;

[0014] Step S3: Add Dendrobium extract, Chrysanthemum extract, plant selenium peptide, protein peptide, vitamin B6, vitamin A, taurine and lutein to the remaining water for injection, stir to dissolve, and obtain a mixed solution.

[0015] Step S4: Combine the pearl powder suspension from step S2 with the mixed medicine solution from step S3, add borneol and sodium hyaluronate, and stir until completely dissolved to obtain the prepared solution.

[0016] Step S5: Adjust the pH of the prepared solution to 6.5-7.5, filter it through a 0.22μm microporous membrane for sterilization, and dispense it into individual containers to obtain the final product.

[0017] Preferably, the method for preparing the pearl powder in step S2 is as follows: the pearl is crushed into coarse powder of 100-200 mesh, water for injection and food-grade lactic acid are added, and the mixture is reacted at 30-40°C for 5-15 hours. After filtration, concentration and crystallization, and low-temperature negative pressure drying, the mixture is then subjected to ultra-fine pulverization to obtain pearl ultra-fine powder with a particle size of 300-500 mesh.

[0018] Preferably, the preparation method of the plant selenium peptide in step S3 is as follows: using selenium-enriched broccoli sprouts or flower heads as raw materials, after freeze-drying and low-temperature pulverization, water is added and cellulase and pectinase are added for enzymatic hydrolysis at 35-50°C for 2-4 hours, then alkaline protease or complex protease is added for enzymatic hydrolysis at 50-55°C for 4-6 hours, after enzyme inactivation and filtration, the peptide is separated and purified using ultrafiltration membranes and nanofiltration membranes with a molecular weight cutoff of less than 3000 Daltons, and finally prepared by freeze-drying or low-temperature spray drying.

[0019] Preferably, the final eye drops are packaged in single-dose packaging, with each vial containing 10–20 ml.

[0020] The technical effects and advantages of this invention are as follows:

[0021] 1. This selenium-containing Dendrobium eye drops for improving eyesight is formulated with a specific weight ratio of Dendrobium extract, chrysanthemum extract, pearl powder, plant selenium peptides, protein peptides, borneol, sodium hyaluronate, lutein, vitamin B6, vitamin A, and taurine. This design achieves a multi-target synergistic effect of trace element anti-oxidation, small molecule peptide tissue repair, vitamin visual nutrition, and traditional Chinese medicine for clearing the liver and improving eyesight. As a result, the eye drops have a higher overall effectiveness in relieving eye fatigue, improving dry eyes, and enhancing visual clarity than traditional single or simple compound eye drops. It can also provide antioxidant protection for the lens and promote corneal epithelial repair.

[0022] 2. This eye drop containing selenium peptides is prepared using a micro-pulverization process combining lactic acid reaction and low-temperature negative pressure drying to produce pearl ultrafine powder with a particle size range of 300-500 mesh. It also uses walnut protein peptides with a molecular weight cutoff of less than 5000 Daltons, which effectively improves the dissolution rate of poorly soluble components and active peptides, as well as corneal permeability. Furthermore, the thickening effect of sodium hyaluronate prolongs the residence time of the solution on the ocular surface, thereby improving the overall bioavailability of the active ingredients.

[0023] 3. This eye drop containing selenium peptides uses a 0.22μm microporous membrane for filtration and sterilization instead of traditional preservatives. Combined with a pH value adjusted to 6.5-7.5, which is isotonic with tears, the eye drop is non-irritating to the eye structure, has high safety for long-term use, and exhibits excellent physicochemical stability, allowing for long-term storage without precipitation or deterioration. Attached Figure Description

[0024] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0025] Figure 1 This is a flowchart of the preparation method of the eye drops containing selenium peptides of the present invention;

[0026] Figure 2 This is a flowchart of the process for preparing plant selenium peptides according to the present invention. Detailed Implementation

[0027] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0028] This embodiment discloses a vision-improving eye drop containing selenium peptides and its preparation method, as shown in the attached figure. Figure 1 To be continued Figure 2 As shown, the core inventive concept of this invention lies in the scientific combination of small molecule plant selenium peptides with specific molecular weights and protein peptides prepared using specific processes with traditional eye-brightening components such as dendrobium, chrysanthemum, and pearl powder, thereby achieving synergistic eye-brightening effects from multiple targets, including anti-oxidation, nutritional supplementation, anti-inflammation, and promotion of corneal repair.

[0029] The selenium peptide-based eye drops prepared according to this method include the following basic components by weight: 10-25 parts of Dendrobium extract, 15-30 parts of chrysanthemum extract, 3-8 parts of pearl powder, 0.01-0.1 parts of plant selenium peptide, 1-3 parts of protein peptide, 1-3 parts of borneol, 2-5 parts of sodium hyaluronate, 0.5-2 parts of lutein, 0.1-0.5 parts of vitamin B6, 0.1-0.5 parts of vitamin A, 0.5-2 parts of taurine, and 80-120 parts of water for injection. It is particularly important to emphasize that the "plant selenium peptide" of this invention is different from the inorganic selenium (such as sodium selenite) that may be used in the prior art. It is a small molecule active substance in a natural chelated state of selenium and peptide, obtained by using selenium-rich plants as raw materials through specific low-temperature compound enzymatic hydrolysis and membrane separation technology, thereby ensuring higher bioavailability and ocular safety.

[0030] Furthermore, in the above formula, Dendrobium extract is used to nourish Yin and tonify the kidneys, clear the liver and improve eyesight, promote basic tear secretion, and improve discomfort such as dry eyes and foreign body sensation. It is especially suitable for those who use their eyes for long periods, suffer from Sjögren's syndrome, or have Yin deficiency constitution. It improves blurred vision. For blurred vision and decreased vision caused by liver and kidney Yin deficiency, Dendrobium can help relieve symptoms of blurred vision by nourishing eye tissues and enhancing retinal function. Its antioxidant components can reduce free radical damage to eye cells, improve eye microcirculation, relieve eye strain and fatigue, and improve blurred vision. For blurred vision and decreased vision caused by liver and kidney Yin deficiency, Dendrobium can help relieve symptoms of blurred vision by nourishing eye tissues and enhancing retinal function. Its antioxidant components can reduce free radical damage to eye cells, improve eye microcirculation, and relieve eye strain and fatigue.

[0031] Dendrobium is used as an adjunct treatment for eye diseases. In the traditional prescription "Dendrobium Night-Shiguang Pill", Dendrobium is used to treat eye diseases such as cataracts and glaucoma. Modern research also shows that it has a certain adjunctive therapeutic effect on diabetic cataracts and macular degeneration, and can slow down the progression of the disease.

[0032] Chrysanthemum extract is used to clear the liver and improve eyesight, as well as to clear heat and reduce inflammation. The combination of pearl powder and borneol can improve eyesight, clear heat and relieve pain, and promote drug penetration, forming the basic eye-improving conditioning unit. Plant selenium peptides are the core efficacy enhancement unit. Selenium is an important component of glutathione peroxidase, which can effectively remove free radicals from the ocular surface and protect the lens and retina. Protein peptides mainly play a role in promoting corneal epithelial repair and healing. Taurine, lutein, vitamin B6, and vitamin A constitute the visual nutrition supplement unit, which nourishes the retina and optic nerve, absorbs harmful blue light, enhances night vision, and improves eye fatigue. Sodium hyaluronate acts as a neurotransmitter regulator, increasing the viscosity of the eye drops, prolonging the residence time of the active ingredients on the ocular surface, and promoting absorption.

[0033] Specifically disclosed is that the Dendrobium extract is an aqueous extract of Dendrobium officinale or Dendrobium nobile, and its preparation method is preferably as follows: take dried Dendrobium stems, crush them, add 8 to 12 times the amount of water for injection, decoct and extract 1 to 3 times, each time for 1 to 2 hours, combine the filtrates, and concentrate to an extract with a relative density of 1.05 to 1.10 to ensure the complete dissolution of the effective components.

[0034] Specifically disclosed is that the chrysanthemum extract is an aqueous extract of wild chrysanthemum or chrysanthemum 'Gongju'. The preferred preparation method is as follows: take dried chrysanthemum, add 10 to 15 times the amount of water for injection, decoct and extract 1 to 2 times, each time for 0.5 to 1.5 hours, filter, and concentrate the filtrate to 1 / 5 to 1 / 10 of the original volume to enrich its heat-clearing and vision-improving active ingredients.

[0035] In particular, to improve the solubility and bioavailability of the active ingredients in pearl powder, it is important to emphasize that the pearl powder used in this invention is an ultrafine powder treated with lactic acid. The preparation method specifically includes: pulverizing pearl powder into coarse powder of 100-200 mesh; adding water for injection and food-grade lactic acid to the coarse powder, reacting at 30-40°C for 5-15 hours, utilizing the solubility of calcium lactate to convert the calcium carbonate in the pearl into an absorbable form; filtering, concentrating and crystallizing, and then drying under low-temperature negative pressure; finally, ultrafine pulverizing the dried material to obtain pearl ultrafine powder with a particle size of 300-500 mesh. This process improves efficacy compared to direct pulverization for drug application.

[0036] It is particularly important to emphasize that the innovative preparation route of the plant selenium peptides in this invention is key to ensuring the high antioxidant activity and safety of the entire eye drop solution. The preparation method is as follows: using the sprouts or flower heads of Enshi high selenium-rich broccoli as raw materials, the plants are first freeze-dried and then pulverized at low temperature (below 40°C) to lock in activity; then targeted enzymatic hydrolysis is performed, with water added and cellulase and pectinase added at 35-50°C for 2-4 hours to fully break the cell walls, followed by alkaline protease or complex protease added at 50-55°C in a neutral environment for 4-6 hours to precisely cleave the large selenoprotein into small selenopeptides; then physical screening is performed, including boiling water bath to inactivate enzymes and centrifugation to remove impurities, followed by ultrafiltration and nanofiltration combined membrane separation technology to collect and enrich components with a molecular weight of less than 3000 Daltons, and finally freeze-drying or low-temperature spray drying to obtain the finished product. The bioavailability and safety of this small molecule plant selenium peptide are far superior to those of inorganic selenium.

[0037] It is particularly important to emphasize that the protein peptides used in this invention are preferably walnut protein peptides. The preparation method is as follows: using cold-pressed walnut cake as raw material, after subcritical low-temperature extraction for defatting and pulverization, water is added and mixed at a material-to-liquid ratio of 1:10 to 1:20; under the conditions of pH 7.0-7.5 and 50-55℃, a complex enzyme of alkaline protease and papain is added for enzymatic hydrolysis for 3-4 hours; after enzymatic hydrolysis, the temperature is raised to 85-95℃ to inactivate the enzyme for 15 minutes, followed by centrifugation and filtration; then, ultrafiltration is used for separation, retaining components with a molecular weight of less than 5000 Daltons, and finally, the product is prepared by freeze drying or low-temperature spray drying. This process directionally obtains the peptides with the strongest antioxidant activity, which can effectively promote corneal epithelial healing and repair.

[0038] As a preferred embodiment, the present invention comprises the following components by weight: 15-20 parts of Dendrobium extract, 20-25 parts of Chrysanthemum extract, 4-6 parts of pearl powder, 0.02-0.05 parts of plant selenium peptide solution, 1-3 parts of protein peptide, 1.5-2.5 parts of borneol, 3-4 parts of sodium hyaluronate, 0.2-0.4 parts of vitamin B6, 0.3-0.4 parts of vitamin A, 0.8-1.5 parts of taurine, and 90-110 parts of water for injection.

[0039] The plant selenium peptide solution is an enzymatic hydrolysis extract of selenium-enriched hydroponic Viola yedoensis. The specific preparation method is as follows: the stems and leaves of selenium-enriched hydroponic Viola yedoensis are crushed and then added with 1:10 of purified water and cellulose and pectinase for enzymatic hydrolysis at a temperature below 50°C. The enzymatic hydrolysate is then purified by membrane filtration and adsorption column to obtain a selenium peptide solution containing selenocysteine.

[0040] The main component of plant selenium peptides is selenocysteine, which is known as one of the 21 amino acids in the human body. It can be directly absorbed and utilized without conversion.

[0041] The eye drops for improving eyesight and their preparation method of the present invention will be described in detail below with reference to specific embodiments, but the scope of protection of the present invention is not limited to the embodiments described.

[0042] Example 1: This example uses a set of preferred efficacy-enhancing formulations to illustrate the preparation process in detail:

[0043] Composition: 15 parts Dendrobium extract, 20 parts Chrysanthemum extract, 5 parts Pearl powder, 0.03 parts Plant selenium peptide, 0.3 parts Protein peptide, 2 parts Borneol, 3.5 parts Sodium hyaluronate, 1.2 parts Lutein, 0.3 parts Vitamin B6, 0.3 parts Vitamin A, 1 part Taurine, 100 parts Water for Injection.

[0044] The preparation process is as follows:

[0045] (1) Pre-treatment of pearl powder: The pearl is crushed into 150-mesh coarse powder, added with water for injection and food-grade lactic acid, reacted at 35°C for 10 hours, filtered, concentrated and crystallized, dried under low temperature and negative pressure, and then ultra-finely pulverized to obtain 400-mesh pearl ultra-fine powder.

[0046] (2) Preparation of suspension: Add the pearl ultrafine powder obtained in step (1) to 20 parts of water for injection and stir at 500 r / min for 15 minutes until a uniform pearl powder suspension is formed.

[0047] (3) Preparation of mixed drug solution: Dendrobium extract, chrysanthemum extract, plant selenium peptide, protein peptide, vitamin B6, vitamin A, lutein and taurine are added to the remaining 80 parts of water for injection and stirred at room temperature until completely dissolved to obtain a clear mixed drug solution.

[0048] (4) Combining and solubilizing: Slowly pour the pearl powder suspension from step (2) into the mixed drug solution from step (3), stir continuously, then add borneol and sodium hyaluronate, and stir for 30 minutes until the system is completely homogeneous.

[0049] (5) Volume adjustment, pH adjustment and sterilization: The total volume of the drug solution is made up to the designed volume with water for injection, the pH value is adjusted to 7.0 with 0.1mol / L sodium hydroxide solution, and sterilized by filtration through a 0.22μm microporous membrane. After the semi-finished product passes the inspection, it is dispensed into 15ml eye drops in a Class 100 clean environment.

[0050] Example 2: This example uses a formula that focuses on basic conditioning and high dendrobium content as an example:

[0051] Composition: 20 parts Dendrobium extract, 25 parts Chrysanthemum extract, 6 parts Pearl powder, 0.05 parts Plant selenium peptide, 0.4 parts Protein peptide, 2.5 parts Borneol, 4 parts Sodium hyaluronate, 1.5 parts Lutein, 0.4 parts Vitamin B6, 0.2 parts Vitamin A, 1.2 parts Taurine, 110 parts Water for Injection.

[0052] The preparation method is the same as in Example 1, except that the pH value is adjusted to 7.2; and in order to improve the uniformity of mixing, a 60°C water bath is used to assist in the dissolution of borneol in step (4).

[0053] Example 3: This example uses a formula that focuses on long-term care and has an appropriate dosage.

[0054] Composition: 12 parts Dendrobium extract, 18 parts Chrysanthemum extract, 4 parts Pearl powder, 0.06 parts Plant selenium peptide, 0.3 parts Protein peptide, 1.5 parts Borneol, 3 parts Sodium hyaluronate, 1.0 part Lutein, 0.2 parts Vitamin B6, 0.2 parts Vitamin A, 0.8 parts Taurine, 90 parts Water for Injection.

[0055] The preparation method is the same as in Example 1, except that the pH value is adjusted to 6.8 and in step (5) it is dispensed into single-dose packages of 10ml each.

[0056] Example 4: This example uses a formula that enhances the repair effect of protein peptides, and is especially suitable for people at high risk of ocular surface damage.

[0057] Composition: 18 parts Dendrobium extract, 22 parts Chrysanthemum extract, 5.5 parts Pearl powder, 0.04 parts Plant selenium peptide, 1 part Protein peptide, 2.2 parts Borneol, 3.8 parts Sodium hyaluronate, 1.8 parts Lutein, 0.3 parts Vitamin B6, 0.4 parts Vitamin A, 1 part Taurine, 105 parts Water for Injection.

[0058] The preparation method is the same as in Example 1, except that the pH value is adjusted to 7.5 and in step (5) it is packaged into single-dose packages of 20ml each.

[0059] To verify the beneficial effects of this invention, the following application tests were conducted:

[0060] 1. Stimulation test

[0061] Ten healthy rabbits were randomly divided into an experimental group and a control group, with five rabbits in each group. The experimental group received eye drops prepared according to Example 1 of this invention in the right eye, 2 drops each time, 3 times a day, for 7 consecutive days. The control group received the same procedure with an equal volume of physiological saline. During the experiment and within 72 hours after the end, the rabbits were observed with a slit lamp and recorded according to the eye irritation response scoring standard. The results showed that no abnormal irritation response such as congestion, edema, or increased secretions was observed in the cornea, iris, or conjunctiva of any rabbit in the experimental group, and the scores were all 0, indicating that the eye drops of this invention are non-irritating to the eyes.

[0062] 2. Efficacy trial of relieving eye strain and dry eye syndrome

[0063] Fifty volunteers, aged 10 to over 80 years old, who required prolonged screen time for daily work and complained of eye fatigue and dryness, were randomly and double-blindly divided into an experimental group and a control group, with 25 participants in each group. The experimental group used the eye drops prepared in Example 1 of this invention, while the control group used commercially available Pearl Brightening Eye Drops. Both groups were used 3 times daily, 1-2 drops each time, for 14 consecutive days. The results were assessed using a combination of symptom scoring scales and patient subjective evaluations. The results showed that the experimental group had a total effective rate of 100% in relieving dryness, soreness, blurred vision, and improving tolerance to prolonged eye use, with significant improvements in visual clarity and color perception. The control group had a total effective rate of 70%. The experiment demonstrates that this invention, through synergistic component analysis, has a significant improvement in efficacy compared to traditional single-component eye drops.

[0064] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A type of eye drops containing selenium peptides for improving eyesight, characterized in that, The product comprises the following components by weight: 10-25 parts Dendrobium extract, 15-30 parts Chrysanthemum extract, 3-8 parts Pearl powder, 0.01-0.1 parts Plant selenium peptide, 1-3 parts Protein peptide, 1-3 parts Borneol, 2-5 parts Sodium hyaluronate, 0.5-2 parts Lutein, 0.1-0.5 parts Vitamin B6, 0.1-0.5 parts Vitamin A, 0.5-2 parts Taurine, and 80-120 parts Water for Injection.

2. The eye drops containing selenium peptides for improving eyesight according to claim 1, characterized in that, The Dendrobium extract is an aqueous extract of Dendrobium officinale or Dendrobium nobile; the Chrysanthemum extract is an aqueous extract of Chrysanthemum indicum or Chrysanthemum indicum.

3. The eye drops containing selenium peptides for improving eyesight according to claim 2, characterized in that, The pearl powder is an ultrafine powder treated with lactic acid reaction. Its preparation method includes: crushing pearl into coarse powder, adding water for injection and food-grade lactic acid to react, filtering, concentrating, drying and then ultrafine pulverizing to obtain pearl ultrafine powder with a particle size of 300-500 mesh.

4. The eye drops containing selenium peptides for improving eyesight according to claim 3, characterized in that, The plant selenium peptide is a small molecule selenium peptide obtained from selenium-enriched broccoli through low-temperature compound enzymatic hydrolysis and membrane separation technology, with a molecular weight of less than 3000 Daltons.

5. The eye drops containing selenium peptides for improving eyesight according to claim 4, characterized in that, The protein peptide is a walnut protein peptide, which is obtained from walnut cake meal through defatting, compound enzymatic hydrolysis and ultrafiltration membrane separation technology, and its molecular weight is less than 5000 Daltons.

6. The eye drops containing selenium peptides for improving eyesight according to claim 5, characterized in that, The eye drops contain no preservatives, are sterilized by filtration through a 0.22μm microporous membrane, and have a pH value of 6.5–7.

5.

7. A method for preparing a vision-improving eye drop containing selenium peptides as described in any one of claims 1-6, characterized in that, Includes the following steps: Step S1: Weigh out the following ingredients according to the specified ratio: Dendrobium extract, Chrysanthemum extract, Pearl powder, Plant selenium peptide, Protein peptide, Borneol, Sodium hyaluronate, Lutein, Vitamin B6, Vitamin A, Taurine, and Water for Injection. Step S2: Add pearl powder to the first part of water for injection and stir to prepare a pearl powder suspension; Step S3: Add Dendrobium extract, Chrysanthemum extract, plant selenium peptide, protein peptide, vitamin B6, vitamin A, taurine and lutein to the remaining water for injection, stir to dissolve, and obtain a mixed solution. Step S4: Combine the pearl powder suspension from step S2 with the mixed medicine solution from step S3, add borneol and sodium hyaluronate, and stir until completely dissolved to obtain the prepared solution. Step S5: Adjust the pH of the prepared solution to 6.5-7.5, filter it through a 0.22μm microporous membrane for sterilization, and dispense it into individual containers to obtain the final product.

8. The method for preparing a selenium-containing eye drop for improving eyesight according to claim 7, characterized in that, The method for preparing pearl powder in step S2 is as follows: pulverize pearl into coarse powder of 100-200 mesh, add water for injection and food-grade lactic acid, react at 30-40℃ for 5-15 hours, filter, concentrate and crystallize, dry under low temperature negative pressure, and then perform ultra-fine pulverization to obtain pearl ultra-fine powder with a particle size of 300-500 mesh.

9. The method for preparing a selenium-containing eye drop for improving eyesight according to claim 8, characterized in that, The preparation method of plant selenium peptides in step S3 is as follows: using selenium-enriched broccoli sprouts or flower heads as raw materials, after freeze-drying and low-temperature pulverization, water is added and cellulase and pectinase are added for enzymatic hydrolysis at 35-50℃ for 2-4 hours, then alkaline protease or complex protease is added for enzymatic hydrolysis at 50-55℃ for 4-6 hours, after enzyme inactivation and filtration, ultrafiltration membrane and nanofiltration membrane with a molecular weight cutoff of less than 3000 Daltons are used for separation and purification, and finally freeze-drying or low-temperature spray drying is performed.

10. The method for preparing a selenium-containing eye drop for improving eyesight according to claim 9, characterized in that, The final eye drops are packaged in single-dose containers, with each container containing 10–20 ml.

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