A synergistic composition for promoting metabolism and a preparation method and application thereof

Abstract

This invention discloses a synergistic composition for promoting metabolism, its preparation method, and its applications, belonging to the field of functional materials technology. The composition comprises a deep-sea mineral extract, synergistic components, a pH adjuster, and an antioxidant. The mineral extract is prepared through modified zeolite / tourmaline adsorption, chelation resin enrichment of trace elements, and micro-electrolysis activation, with calcium, magnesium, and potassium retention rates ≥85% and vanadium, chromium, selenium, zinc, and molybdenum retention rates ≥70%. The synergistic components are selected from polysaccharides, plant extracts, and peptides. This composition can be formulated into oral liquid beverages, solid beverages, or tablets. Experiments have shown that it can effectively inhibit α-glucosidase, improve insulin resistance, reduce blood glucose and lipids in diabetic mice, and significantly increase the basal metabolic rate and reduce body fat percentage in humans. It can achieve functions such as promoting metabolism, assisting in lowering blood sugar and lipids, enhancing skin cell activity, anti-oxidation, promoting collagen synthesis, improving skin moisture and elasticity, and anti-aging.

Description

Technical Field

[0001] This invention relates to the field of functional materials technology, and in particular to a synergistic composition that promotes metabolism, its preparation method, and its application. Background Technology

[0002] Metabolic disorders are a core contributing factor to metabolic syndrome, including obesity, hyperglycemia, and hyperlipidemia. With changing lifestyles, these health problems are becoming increasingly prevalent, creating a growing demand for safe and effective metabolic conditioning products. Deep ocean water typically refers to seawater layers located 200 meters below sea level, where sunlight cannot reach. Due to its unique formation environment, deep ocean water is characterized by low temperatures, cleanliness, rich nutrient content, and stable composition. It is rich in macro-minerals such as calcium, magnesium, potassium, and sodium, as well as dozens of trace elements crucial for human metabolism, including vanadium, chromium, selenium, zinc, and molybdenum. These elements exist primarily in free ion form, theoretically possessing extremely high bioavailability.

[0003] However, existing metabolic conditioning products using deep-sea water as raw material have several limitations: On the one hand, traditional pretreatment processes (such as sand filtration and reverse osmosis) indiscriminately remove beneficial mineral ions, especially trace active elements such as vanadium, chromium, and selenium, while purifying impurities, leading to the loss of the core nutritional value of deep-sea water; on the other hand, even processes that partially retain minerals often fail to maintain the high activity of mineral ions. Inorganic ions are prone to aggregation and precipitation during separation, concentration, or long-term storage, resulting in a significant decrease in their bioavailability; furthermore, while single deep-sea water mineral extracts are rich in nutrients, their effects on targeted regulation of glucose and lipid metabolism and improvement of overall metabolic efficiency are relatively mild, lacking synergistic effects with other active ingredients. In addition, existing technologies often focus on deep-sea water pretreatment processes as an independent technical focus, neglecting their correlation with the final metabolic conditioning composition, resulting in insufficient compatibility between the pretreatment process and synergistic components, failing to fully leverage the synergistic effects of mineral ions and active ingredients. In addition, existing metabolic conditioning products rarely focus on the relationship between skin cell activity and the overall metabolic state, and lack a multi-dimensional synergistic design scheme that ranges from improving metabolism and anti-oxidation to directly enhancing skin cell vitality.

[0004] Therefore, it is of great significance and value to design a targeted deep-sea water pretreatment process, combine it with other synergistic active components for scientific compounding, prepare a synergistic composition that promotes metabolism, and expand its application in beauty and skin cell activity. Summary of the Invention

[0005] The purpose of this invention is to provide a synergistic composition for promoting metabolism, its preparation method, and its application. This composition uses deep-sea water mineral extract obtained through a specific pretreatment process as the active base material, combined with synergistic components, to significantly improve the absorption and utilization rate of nutrients, efficiently promote the body's metabolism, and achieve physiological functions such as assisting in lowering blood sugar and lipids. At the same time, it can significantly enhance skin cell activity, promote the proliferation of skin fibroblasts, reduce oxidative damage, improve skin moisture and elasticity, and exert beauty and anti-aging effects.

[0006] To achieve the above objectives, the present invention provides a synergistic composition for promoting metabolism, comprising the following components: Active base material: mineral extracts from deep-sea water; Synergistic components: one or more active substances from polysaccharides, plant extracts, and peptides; pH adjuster: one or more of citric acid, sodium citrate, tartaric acid, malic acid, lactic acid, and disodium hydrogen phosphate; Antioxidants: Vitamin C or Vitamin E; Among them, the deep-sea water mineral extract is obtained through the following pretreatment process: S1. The primary purified water is obtained by sedimentation and filtration of the raw deep ocean water. The primary purified water is then passed through a filter column filled with modified zeolite and tourmaline composite filter media to obtain secondary purified water. S2. Take 10%-30% of the volume of the secondary purified water, pass it through a chromatographic column loaded with chelating resin, and then elute it with food-grade citric acid solution. Backfill the elution solution into the remaining part of the secondary purified water and mix it evenly to obtain element-retained water. S3. After micro-electrolysis of the element-retaining water, pretreated deep-sea water is obtained. This pretreated water is then concentrated to 1 / 10-1 / 2 of its original volume to obtain a deep-sea water mineral extract. In this deep-sea water mineral extract, the retention rates of calcium, magnesium, and potassium are not less than 85% of the original deep-sea water, and the retention rates of vanadium, chromium, selenium, zinc, and molybdenum are not less than 70% of the original deep-sea water.

[0007] Preferably, the mass ratio of modified zeolite to tourmaline in the composite filter media is 3:1-5:1. The modified zeolite is obtained by soaking natural zeolite in a 0.5-1.0 mol / L sodium chloride solution for 12-24 hours, followed by washing and drying.

[0008] Preferably, the chelating resin is an iminodiacetic acid type or an aminophosphonic acid type chelating resin.

[0009] Preferably, in the micro-electrolysis process, the DC voltage is 0.5-2.0V and the current density is 5-20A / m. 2 The processing time is 10-30 minutes.

[0010] Preferably, the product contains 100-200 parts of deep-sea mineral extract and 5-30 parts of synergistic components, wherein the mass ratio of polysaccharides, plant extracts and peptides is 2-5:1-3:1.5-4.

[0011] Preferably, the polysaccharides are one or more of the following: brown algae polysaccharide sulfate, chitosan oligosaccharide, and oat β-glucan; the plant extracts are one or more of the following: bitter melon extract, mulberry leaf extract, and cinnamon extract; and the peptides are one or more of the following: corn oligopeptides, soybean peptides, and collagen peptides.

[0012] The present invention also provides the application of the above-mentioned synergistic composition for promoting metabolism, wherein the synergistic composition is used in the preparation of oral liquid beverages, solid beverages, foods or medicines for promoting metabolism, assisting in lowering blood sugar and blood lipids, enhancing skin cell activity, anti-oxidation, promoting collagen synthesis, improving skin moisture and elasticity, and anti-aging functions.

[0013] Preferably, when the synergistic composition is used to prepare a liquid beverage, the following steps are specifically included: (1) Add the synergistic components to a portion of the deep-sea mineral extract at a mass ratio of 1:1 to 3:1, and stir to dissolve at 40-60°C to form a premix; (2) Mix the premixed solution with the remaining deep-sea mineral extract, add a pH adjuster to adjust the pH to 6.5-8.0, and add an antioxidant and water; (3) Stir at room temperature for 30-60 minutes to fully complex and stabilize the mixture, then homogenize the mixture at 15-25 MPa pressure, and then sterilize to obtain the target product.

[0014] Preferably, when the synergistic composition is used to prepare a solid beverage, the following steps are specifically included: (1) Mineral powder is prepared by spray drying of deep-sea water mineral extract, and the total mineral content in the mineral powder is ≥15%; (2) Mix the mineral powder with the synergistic components, antioxidants and pH adjusters in proportion, put them into a mixer and mix at room temperature for 30-60 minutes until uniform; (3) Package the mixed powder to obtain a solid beverage.

[0015] Preferably, when the synergistic composition is used to prepare a pharmaceutical product, the following steps are specifically included: (1) Mineral powder is prepared by spray drying of deep-sea water mineral extract, and the total mineral content in the mineral powder is ≥15%; (2) Mix the mineral powder with the synergistic components, pH adjuster and antioxidant evenly, add purified water to granulate, dry at 40-60℃ until the moisture content is ≤5%, and granulate. (3) Add lubricant, mix well, compress into tablets to obtain tablets.

[0016] The deep-sea mineral extract in the composition of this invention employs a three-stage combined extraction process: selective retention of macro-ions, enrichment of trace elements, and ion activation. This process efficiently removes harmful heavy metals while fully preserving beneficial macro-ions such as calcium, magnesium, and potassium. Furthermore, through chelation resin enrichment technology, trace elements (V, Cr, Se, Mo, etc.) that are easily lost in traditional processes are captured and refilled, achieving highly efficient retention of the entire element spectrum, from macro to trace and from common to rare. The retention rate of key trace elements has increased from less than 20% in traditional processes to over 70%, laying a unique material foundation for the synergistic effect of the composition. Simultaneously, gentle micro-electrolysis optimizes the redox potential of the water, promoting the formation of more stable, transmembrane-transportable small molecule ion clusters or complexes of mineral ions, significantly improving the stability and absorption rate of minerals in the gastrointestinal environment.

[0017] This invention scientifically combines deep-sea mineral extracts rich in a complete spectrum of active ions with polysaccharides, plant extracts, and peptides from pure natural sources. On one hand, the balanced ion composition in the extracts (such as the Mg / Ca ratio and Na / K ratio) creates a stable ion microenvironment for human cells. Elements such as V, Cr, and Zn act as key cofactors, directly participating in and activating the insulin signaling pathway and the activity of enzymes related to glucose and lipid metabolism. On the other hand, the synergistic components, such as fucoidan, bitter melon saponins, and corn oligopeptides, not only independently exert hypoglycemic, lipid-lowering, and antioxidant effects, but their hydroxyl, carboxyl, and amino groups can also form soluble complexes with mineral ions, achieving targeted delivery and protection. This synergy between natural components derived from marine and terrestrial plants produces a significant synergistic effect. In addition, when the pH of the composition is maintained between 6.5 and 8.0, mineral ions in deep ocean water (especially vanadium, molybdenum, etc.) can form stable soluble complexes with the hydroxyl and carboxyl groups in the synergistic components, avoiding precipitation or complex instability problems that may occur under neutral or acidic conditions, thereby ensuring the bioactivity of the composition during its shelf life.

[0018] Furthermore, the synergistic composition of this invention, through the scientific compounding of deep-sea mineral extracts (rich in trace elements such as selenium, zinc, and molybdenum) with polysaccharides, plant extracts, peptides, and antioxidants, has been demonstrated in in vitro cell, biochemical, and human trials to significantly promote the proliferation of human skin fibroblasts and improve cell survival rate; effectively reduce UV-induced reactive oxygen species levels in skin cells, enhance the activity of superoxide dismutase and glutathione peroxidase, and reduce malondialdehyde production, thereby alleviating oxidative stress damage; simultaneously upregulate type I collagen expression and inhibit matrix metalloproteinase-1 activity, increase hydroxyproline content, and promote collagen synthesis; on human skin, continuous use can increase the moisture content of the stratum corneum, reduce transepidermal water loss, reduce the area and depth of wrinkles, and improve skin barrier function and moisturizing ability. These results indicate that the composition of this invention possesses multiple cosmetic effects, including antioxidant, anti-photodamage, collagen synthesis promotion, moisturizing, and anti-wrinkle properties, and can be used to prepare foods, health foods, cosmetics, or pharmaceuticals that enhance skin cell activity, delay skin aging, and improve skin health.

[0019] All components of this invention are derived from the ocean or natural plants, with no chemically synthesized additives, which aligns with the market trend of natural and healthy products and can be flexibly applied to various product forms.

[0020] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0021] Figure 1 This is a graph showing the comparison of the inhibition rates of α-glucosidase by the synergistic composition of the present invention and each control group; Figure 2 This is a graph showing the comparison of glucose consumption between the synergistic composition of the present invention and each control group in an insulin-resistant HepG2 cell model. Figure 3 This is a graph showing the comparison of fasting blood glucose (FBG), total cholesterol (TC), and triglyceride (TG) levels in diabetic model mice using the synergistic composition of the present invention and various control groups. Detailed Implementation

[0022] The technical solution of the present invention will be further described below with reference to the accompanying drawings and embodiments. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention should be considered equivalent substitutions and are included within the protection scope of the present invention. Furthermore, it should be understood that after reading the contents of this invention, those skilled in the art can make various alterations or modifications to the invention, and these equivalent forms also fall within the scope defined by the appended claims and are all within the protection scope of the present invention.

[0023] In this document, the term "embodiment" means that a specific feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The term "embodiment" appearing in various places throughout the specification does not necessarily refer to the same embodiment, nor does it specifically limit its independence or connection with other embodiments. In principle, in this application, as long as there are no technical contradictions or conflicts, the technical features mentioned in each embodiment can be combined in any way to form corresponding implementable technical solutions.

[0024] Unless otherwise defined, the technical terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains; the use of related terms herein is merely for the purpose of describing particular embodiments and is not intended to limit this application.

[0025] Unless otherwise specified, the reagents, instruments, and equipment used in this invention are all commonly used by those skilled in the art, and the testing standards all use national or international standards commonly used in the field, without further explanation.

[0026] The corn oligopeptides used in this invention refer to a mixture of oligopeptides obtained from corn protein through enzymatic hydrolysis, separation, purification, and drying processes. Their quality indicators are as follows: protein content ≥ 80% (dry basis), oligopeptide content ≥ 75% (dry basis), and peptides with a relative molecular mass ≤ 1000 Da accounting for ≥ 90% of the protein hydrolysate. The determination of these indicators can be based on the industry standard QB / T 4707-2014 "Corn Oligopeptide Powder". Furthermore, in solid or semi-solid formulations, pH adjusters mainly function as acidity regulators to improve product flavor or provide a stable microenvironment for active ingredients.

[0027] Example 1 This embodiment provides a method for extracting minerals from deep ocean water, including the following steps: S1. Raw water collection and primary purification: Deep ocean water is collected from a certain area of ​​the Pacific Ocean at a depth of 500 meters below sea level. The water is allowed to settle in a sedimentation tank for 24 hours to remove large particles of sediment. Then, the water is passed through 50μm, 10μm, and 1μm polypropylene melt-blown filter cartridges in sequence to obtain primary purified water.

[0028] S2. Selective Retention of Macro-ions and Adsorption of Heavy Metals: Primary purified water is passed through a filter column filled with composite filter media at a flow rate of 2 times the bed volume / hour to obtain secondary purified water. The composite filter media consists of sodium chloride-modified zeolite and tourmaline in a 4:1 mass ratio. The modified zeolite is prepared by soaking natural zeolite in a 1.0 mol / L NaCl solution for 24 hours, followed by washing and drying.

[0029] S3. Trace element enrichment: Take 20% of the total secondary purified water and pass it through a chromatographic column (2L column volume) packed with iminodiacetic chelating resin (model: AmberliteIRC748) at a flow rate of 2 times the bed volume / hour. After the resin is saturated, it is eluted with 1.0mol / L food-grade citric acid solution, and the eluent is collected. The eluent is uniformly backfilled into the remaining 80% of the secondary purified water and mixed evenly to obtain element-retained water.

[0030] S4. Ionization and Activation Treatment: The obtained element-retaining water is introduced into a micro-electrolysis cell, using a ruthenium-iridium coated titanium anode as the anode and stainless steel as the cathode, with an applied voltage of 1.2V and a current density of 10A / m. 2 The water is collected after a 20-minute treatment period, which is the pretreated deep-sea water.

[0031] S5. Concentration: The pretreated deep-sea water is concentrated to 1 / 5 of its original volume by low-temperature vacuum evaporation to obtain a 5-fold concentrated deep-sea water mineral extract (denoted as DOW-1).

[0032] Testing revealed that the total mineral content in DOW-1 was 1180 mg / L. Compared to the raw water, the retention rates for calcium, magnesium, and potassium were 91.5%, 93.2%, and 88.7%, respectively; the retention rates for vanadium, chromium, selenium, zinc, and molybdenum were 72.3%, 75.1%, 78.6%, 82.5%, and 71.4%, respectively. The removal rates for harmful heavy metals (lead, cadmium, and mercury) were all above 96%.

[0033] Example 2 This embodiment provides the preparation of a synergistic composition (liquid beverage) that promotes metabolism. The formulation (based on 1000 kg of finished product) includes: Active base material: DOW-1150kg prepared in Example 1; Synergistic components: 6 kg of brown algae polysaccharide sulfate, 3 kg of bitter melon extract (bitter melon saponin content ≥10%), and 5 kg of corn oligopeptides; Antioxidant: Vitamin C 0.6kg; pH adjuster: 0.8 kg citric acid, 0.2 kg sodium citrate; Flavor improver: 20 kg of erythritol; The remainder is purified water.

[0034] Its preparation method includes the following steps: (1) Take 28kg of DOW-1 and heat it to 50℃. Add brown algae polysaccharide sulfate, bitter melon extract and corn oligopeptide, and stir until completely dissolved to obtain a premixed solution.

[0035] (2) Mix the premixed solution with the remaining 122kg DOW-1 concentrate and erythritol, add citric acid and sodium citrate to adjust the pH to 7.0, and add vitamin C and water.

[0036] (3) Stir at room temperature for 50 min to fully complex and stabilize the mixture, then place it under 20 MPa pressure for homogenization and treatment, and then sterilize it with UHT (135℃, 5s), and aseptically fill it to obtain the finished beverage composition (50 mL per bottle).

[0037] Example 3 This embodiment provides the preparation of a synergistic composition (solid beverage) that promotes metabolism. The formulation (based on 1000 kg of finished product) includes: Active base material: 200 kg of DOW-1 mineral powder (prepared by spray drying, with a total mineral content ≥15%) obtained in Example 1; Synergistic components: 12 kg chitosan oligosaccharide, 8 kg mulberry leaf extract (DNJ content ≥2%), 15 kg soybean peptide; Antioxidant: Vitamin C 5kg; Filler: 750 kg of maltodextrin; pH adjustment agent: 7.5 kg of citric acid; Other excipients: 0.5 kg of sucralose.

[0038] Its preparation method includes the following steps: (1) Add 200kg DOW-1 mineral powder, 12kg chitosan oligosaccharide, 8kg mulberry leaf extract, 15kg soybean peptide, 5kg vitamin C, 750kg maltodextrin, 0.5kg sucralose and 7.5kg citric acid into a three-dimensional mixer in proportion.

[0039] (2) Mix at room temperature for 45 minutes until homogeneous to obtain mixed powder.

[0040] (3) Package the mixed powder into 10g bags to obtain the finished solid beverage product.

[0041] Example 4 This embodiment provides the preparation of a synergistic composition (oral tablet) that promotes metabolism, the formulation (600mg per tablet based on 10,000 tablets) comprising: Active base material: 800g of DOW-1 mineral powder (prepared by spray drying, with a total mineral content ≥15%) obtained in Example 1; Synergistic components: 200g of brown algae polysaccharide sulfate, 100g of bitter melon extract (bitter melon saponin content ≥10%), and 180g of corn oligopeptides; Antioxidant: Vitamin C 30g; Filler: 2500g of microcrystalline cellulose; Adhesive: Polyvinyl Acetate K30 100g; Disintegrant: 150g of croscarmellose sodium cellulose; Lubricant: 40g magnesium stearate; pH adjuster: 50g citric acid, 50g sodium citrate.

[0042] Its preparation method includes the following steps: (1) Take 800g DOW-1 mineral powder, 200g brown algae polysaccharide sulfate, 100g bitter melon extract, 180g corn oligopeptide, 30g vitamin C, 2500g microcrystalline cellulose, 100g povidone K30, 150g crosslinked carboxymethyl cellulose sodium, 50g citric acid, and 50g sodium citrate, and put them into a wet mixing granulator and mix for 15 minutes until uniform.

[0043] (2) Add 500mL of pure water as a wetting agent, continue stirring and granulating to obtain soft material. Granulate the soft material through a 20-mesh sieve, place it in a fluidized bed dryer, and dry it at 50℃ until the moisture content is ≤3%.

[0044] (3) After drying, the granules are granulated through an 18-mesh sieve, 40g of magnesium stearate is added, and the mixture is mixed for 5 minutes. The total granules are placed in a tablet press, and the pressure is adjusted to make the tablet weight 600mg. The tablets are then compressed to obtain the finished tablet product.

[0045] Comparative Example 1 (Traditional RO Treatment) This comparative example uses conventional reverse osmosis to treat the same deep-sea water source as in Example 1, obtaining RO purified water (mineral removal rate >98%). This RO purified water is concentrated at the same concentration factor (5 times) as in Example 1, serving as a substitute for mineral extract. Then, it is compounded according to the formulation ratio of Example 2 (including 150 kg of this RO concentrate and the same amount of synergistic components and excipients) to prepare a control beverage.

[0046] Comparative Example 2 (without enrichment of trace elements) This comparative example uses the same process as Example 1, but omits step S3 (trace element enrichment) and uses only the water treated in steps 1, 2, 4, and 5 (i.e., purified water without trace element supplementation) as the base material. Then, it is compounded according to the formulation ratio of Example 2 to obtain the control beverage.

[0047] Comparative Example 3 (Minerals Only) This comparative example uses DOW-1 prepared in Example 1, without adding any synergistic components, and is flavored only with erythritol and acidulants to prepare a control beverage.

[0048] Comparative Example 4 (without micro-electrolysis) This comparative example uses the same process as Example 1, but omits the micro-electrolysis treatment in step S4. Instead, the element-retaining water is directly concentrated to 1 / 5 of its original volume through low-temperature vacuum evaporation to obtain a deep-sea water mineral extract. Then, a control beverage is prepared according to the formulation and preparation method of Example 2.

[0049] To verify the efficacy of the synergistic composition of the present invention, functional evaluations were conducted at the in vitro, cellular, animal, and human levels, respectively.

[0050] 1. In vitro α-glucosidase inhibition experiment Using acarbose as a positive control, the inhibition rate of α-glucosidase in the seven groups of samples from Examples 2-4 and Comparative Examples 1-4 was tested. Each sample was diluted with phosphate buffer (pH 6.8) to the same total solids concentration (5 mg / mL). 50 μL of sample was mixed with 50 μL of α-glucosidase solution (0.2 U / mL), incubated at 37°C for 10 min, and then 50 μL of 4-nitrophenyl-α-D-glucopyranoside (PNPG, 1 mmol / L) was added. The reaction was continued for 20 min, and then 100 μL of Na₂CO₃ (0.2 mol / L) was added to terminate the reaction. The absorbance was measured at 405 nm, and the inhibition rate was calculated. Results are as follows: Figure 1 As shown in Table 1 below.

[0051] Table 1: Inhibition rate of α-glucosidase in each group (%)

[0052] Depend on Figure 1 As shown in Table 1, the inhibition rates of α-glucosidase in Examples 2-4 were 82.1%, 79.6%, and 81.3%, respectively, all significantly higher than those in Comparative Examples 1-4. Furthermore, there was no significant difference among the three example groups, indicating that the synergistic compositions of different dosage forms of the present invention can effectively inhibit α-glucosidase activity. Moreover, although the inhibition rate of Comparative Example 4 was higher than that of Comparative Examples 2 and 3, it was still significantly lower than that of the other example groups, indicating that microelectrolysis treatment plays an important role in fully activating the bioactivity of the mineral extract and thus achieving efficient synergy with the synergistic components.

[0053] 2. Cellular level insulin resistance improvement experiment A HepG2 cell model of insulin resistance was established. HepG2 cells were cultured in DMEM medium containing 10% fetal bovine serum. When the cells reached 80% confluence, 0.5 mmol / L palmitic acid was added for 24 h to induce insulin resistance. Then, the seven samples mentioned above (all diluted to a total solids concentration of 0.5 mg / mL) were added for 24 h, and the culture medium was collected. The glucose consumption in the culture medium was detected using the glucose oxidase method. Normal cells were used as a blank control, and model cells (without sample addition) were used as a negative control. Results are as follows: Figure 2 As shown in Table 2 below.

[0054] Table 2. Cellular glucose consumption (mmol / L) in each group

[0055] Depend on Figure 2 As shown in Table 2, the glucose consumption of the treatment groups in Examples 2-4 was 2.8 times, 2.6 times, and 2.7 times that of the model group, respectively, all significantly higher than that of the comparative groups, and there was no significant difference among the three example groups. This indicates that the composition of the present invention can significantly improve cellular insulin resistance, promote glucose uptake and utilization, and the effect is independent of the specific dosage form. Moreover, although the glucose consumption of Comparative Example 4 was higher than that of Comparative Examples 2 and 3, it was still significantly lower than that of the three example groups. This result further confirms that microelectrolysis treatment can enhance the bioactivity of mineral ions, thereby strengthening their synergistic effect with polysaccharides, plant extracts, and peptides in improving insulin resistance.

[0056] 3. Animal experiments on lowering blood sugar and lipids A type 2 diabetic mouse model induced by a high-fat diet combined with streptozotocin (STZ) was established. Ninety SPF-grade male C57BL / 6 mice were randomly divided into nine groups: normal control group, model group, Example 2 group, Example 3 group, Example 4 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, and Comparative Example 4 group, with 10 mice in each group. Except for the normal control group, the other groups were fed a high-fat diet (containing 20% ​​sucrose, 10% lard, and 2.5% cholesterol) for 4 weeks, followed by intraperitoneal injection of STZ (50 mg / kg) for 3 consecutive days to induce the diabetic model. After successful model establishment, each treatment group was administered the corresponding sample by gavage (liquid beverages were administered directly by gavage; solid beverages and tablets were prepared into solutions or suspensions of the same concentration, with the dosage converted to an equivalent amount of active base and synergistic component according to Example 2, with a gavage volume of 10 mL / kg body weight). The model group and normal control group were administered an equal volume of distilled water by gavage, once daily for 8 consecutive weeks. Fasting for 12 hours after the last dose was performed, and fasting blood glucose (FBG) was measured. Blood was collected, serum was separated, and total cholesterol (TC) and triglycerides (TG) were measured. Results are as follows: Figure 3 As shown in Table 3 below.

[0057] Table 3 Fasting blood glucose and blood lipid levels in mice of each group

[0058] Depend on Figure 3 As shown in Table 3, compared with the model group, the fasting blood glucose, TC, and TG levels of mice in Examples 2-4 were significantly reduced, and there were no significant differences among the three example groups. All of these levels were significantly better than those of the comparative example groups. This result indicates that the composition of the present invention has a synergistic effect in assisting blood glucose and lipid reduction at the whole animal level, and different dosage forms can stably exert their efficacy. Furthermore, the FBG, TC, and TG levels in Comparative Example 4 were lower than those in Comparative Examples 2 and 3, but significantly higher than those in the three example groups. This result indicates that even with the complete trace element spectrum preserved, the blood glucose and lipid reduction effect of the composition is significantly weakened after omitting the microelectrolysis step, proving that microelectrolysis is a key step in achieving deep synergistic effects between the full spectrum of minerals and synergistic components.

[0059] 4. Evaluation of the effect of basal metabolic rate improvement 140 participants aged 25-55 with a body mass index (BMI) between 22 and 28 kg / m² were selected. 2 Healthy subjects without serious heart, liver, kidney, or metabolic diseases, and who had not taken any drugs or health supplements affecting metabolism within the past 3 months, were randomly divided into 7 groups of 20 subjects each: Example 2 group, Example 3 group, Example 4 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, and Comparative Example 4 group. Each group consumed / took the corresponding sample: Example 2 group: Drink 550mL of the liquid beverage of Example 2 daily (divided into two doses, 275mL each time, in the morning and evening); Example 3 group: Take the solid beverage powder from Example 3 daily and mix it with 500mL of warm water (divided into two doses, each equivalent to 5g of powder). Example 4 group: Take 2 tablets (600mg each) of Example 4 orally twice a day with warm water. Comparative Examples 1-4: The dosage form and dosage were consistent with those of Example 2 group. The intervention lasted for 8 consecutive weeks. During the intervention, the patient maintained a regular diet and daily activity level, without any additional exercise.

[0060] Detection indicators: Before and after the intervention, the basal metabolic rate (BMR, unit: kcal / day) of the subjects was measured using the indirect calorimetry method; at the same time, body fat percentage (%) and muscle mass (kg) were measured using bioelectrical impedance analysis (BIA). The results are shown in Table 4.

[0061] Table 4. Changes in basal metabolic rate, body fat percentage, and muscle mass before and after intervention in each group of subjects.

[0062] As shown in Table 4, after 8 weeks of intervention, the BMR of groups 2-4 was significantly higher than before the intervention, body fat percentage was significantly lower, and muscle mass was slightly increased. The increase in BMR in Comparative Example 4 was significantly lower than that of the other example groups, but higher than that of Comparative Examples 2 and 3. These results indicate that microelectrolysis treatment can significantly enhance the composition's ability to increase basal metabolic rate and reduce body fat percentage, making it an indispensable technical means to achieve the synergistic effect of this invention. Furthermore, there were no significant differences among the three example groups, indicating that the synergistic composition of this invention can effectively increase the human basal metabolic rate, reduce body fat percentage, and increase muscle mass. Its effect depends on the combined action of the full spectrum of minerals and synergistic components, and different dosage forms can stably achieve this efficacy.

[0063] 5. Evaluation of cosmetic and skin cell activity effects 5.1 In vitro skin cell proliferation activity assay Collect human skin fibroblasts (HSF) in the logarithmic growth phase at a concentration of 1 × 10⁻⁶. 4 Cells were seeded per well in 96-well plates. After culturing for 24 hours, the original culture medium was discarded, and samples from Examples 2-4 were added, diluted with DMEM medium to a total solids concentration of 0.1, 0.5, and 1.0 mg / mL, respectively. A blank control group (culture medium only) and comparative groups 1-4 (same concentration) were also set up. Each group had 5 replicates. After culturing for another 48 hours, 10 μL of CCK-8 solution was added to each well, and the cells were incubated for 2 hours. The absorbance was measured at 450 nm. The cell viability (%) was calculated with the blank control group as 100%. The results are shown in Table 5.

[0064] Table 5: Cell viability

[0065] As shown in Table 5, the HSF cell survival rates of the samples in Examples 2-4 at a concentration of 0.5 mg / mL increased to 142%, 138%, and 145% of the blank control group, respectively, which were significantly higher than those of the comparative groups 1-4. This indicates that the composition of the present invention can significantly promote the proliferation of skin fibroblasts, and the effect depends on the complete process and synergistic components.

[0066] 5.2 Skin cell antioxidant and UVB damage resistance experiments Human keratinocytes (HaCaT) were seeded into 6-well plates. When the confluence reached 70%, DMEM medium containing 0.5 mg / mL of the samples from Examples 2-4 or comparative examples (sample group) and an equal volume of DMEM medium without samples (blank control group) were added for pretreatment for 24 h. After pretreatment, each group was treated with 30 mJ / cm² water. 2Cells were irradiated with UVB, collected, and reactive oxygen species (ROS) levels were detected using the DCFH-DA probe. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) content were determined according to the kit method. The results are shown in Table 6.

[0067] Table 6: Comparison of oxidative stress indices in skin cells among different groups (UVB model)

[0068] As shown in Table 6, compared with the blank control group and each comparative group, the ROS fluorescence intensity and MDA content of Examples 2-4 were significantly reduced, while the SOD and GSH-Px activities were significantly increased. All indicators were significantly better than those of all comparative groups. The above results indicate that the composition of the present invention, through full-spectrum mineral retention, micro-electrolysis activation treatment, and scientific compounding with synergistic components, can synergistically enhance the antioxidant defense capacity of skin cells and effectively reduce UVB-induced oxidative stress damage. Moreover, each process step and component is indispensable.

[0069] 5.3 Detection of Collagen Synthesis Promotion Effect HSF cells were treated with 0.5 mg / mL of the samples from each example and comparative example for 48 h. Total protein was extracted and Western blot was performed. Type I collagen (COL1A1) and matrix metalloproteinase-1 (MMP-1) were used, with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an internal control. The relative expression levels of proteins were calculated by chemiluminescence color development and gray value analysis. At the same time, cell supernatant was collected, and the hydroxyproline content was determined using a hydroxyproline kit (alkaline hydrolysis method). The blank control group (culture medium only) was used as the baseline. The results are shown in Table 7.

[0070] Table 7: Effects on COL1A1 and MMP-1 expression and hydroxyproline content in HSF cells

[0071] As shown in Table 7, compared with the blank control group, the Examples 2-4 groups significantly upregulated the expression of type I collagen in HSF cells, significantly inhibited the expression of matrix metalloproteinase-1, and greatly increased the hydroxyproline content. The effects of all comparative groups were significantly weaker than those of the Example groups. While Comparative Example 4 was better than the other comparative examples, it was still significantly lower than the Example groups. The effects of Comparative Examples 2 and 3 further decreased, and Comparative Example 1 had almost no promoting effect. This indicates that the composition of the present invention, through full-spectrum mineral retention, micro-electrolysis activation, and combination with synergistic components, can synergistically promote collagen synthesis and inhibit its degradation, and each process step and component is indispensable.

[0072] 5.4 Short-term human skin trial Sixty healthy female subjects, aged 30-55 years, with mild to moderate facial skin dryness and fine lines, were recruited. Each group of 15 subjects was randomly assigned to one of the following groups: Example 2, Example 3, Example 4, and a matrix control group (blank gel without active base material and synergistic components). Subjects applied the corresponding sample gel to their face morning and evening after cleansing for 8 consecutive weeks. Skin stratum corneum moisture content was measured using a Corneometer CM825 before use and after 8 weeks of use. Transepidermal water loss (TEWL) was measured using a Tewameter TM300, and the area and depth of crow's feet were analyzed using a Visioface VL1000. The rate of change for each indicator was calculated, and the results are shown in Table 8.

[0073] Table 8: Change rates of various indicators after 8 weeks of human skin trial

[0074] As shown in Table 8, after 8 weeks of continuous use, the skin stratum corneum moisture content of subjects in Examples 2-4 increased by 20.8%~23.1%, transepidermal water loss decreased by 17.2%~19.0%, and the area of ​​crow's feet wrinkles decreased by 26.4%~31.5%, while the matrix control group showed basically no change in any indicators. The improvement effects of Comparative Examples 1-4 were all much lower than those of the Example groups. Although the effect of Comparative Example 4 was better than the other comparative examples, it was still significantly weaker than that of the Example groups. The above results demonstrate that the composition of the present invention can effectively improve skin barrier function, enhance moisturizing ability, and reduce wrinkles. Its cosmetic efficacy depends on the complete mineral extraction process and the scientific compounding with synergistic components.

[0075] In summary, the synergistic composition for promoting metabolism provided by this invention, through the deep synergistic effect of multiple mineral retention and natural synergistic components, can effectively inhibit α-glucosidase activity, improve insulin resistance, reduce fasting blood glucose and blood lipids in diabetic mice, and significantly increase the basal metabolic rate and reduce body fat percentage in healthy humans. Simultaneously, this composition can also significantly promote the proliferation of skin fibroblasts, enhance the antioxidant capacity of skin cells, inhibit UVB-induced oxidative damage, promote collagen synthesis, improve skin moisture content, reduce transepidermal water loss, and reduce wrinkles, demonstrating clear cosmetic and skin cell activity-enhancing effects.

[0076] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A synergistic composition for promoting metabolism, characterized in that, It contains the following components: Active base material: mineral extracts from deep-sea water; Synergistic components: one or more active substances among polysaccharides, plant extracts, and peptides; pH adjuster: one or more of citric acid, sodium citrate, tartaric acid, malic acid, lactic acid, and disodium hydrogen phosphate; Antioxidants: Vitamin C or Vitamin E; Among them, the deep-sea water mineral extract is obtained through the following pretreatment process: S1. The primary purified water is obtained by sedimentation and filtration of the raw deep ocean water. The primary purified water is then passed through a filter column filled with modified zeolite and tourmaline composite filter media to obtain secondary purified water. S2. Take 10%-30% of the volume of the secondary purified water, pass it through a chromatographic column loaded with chelating resin, and then elute it with food-grade citric acid solution. Backfill the elution solution into the remaining part of the secondary purified water and mix it evenly to obtain element-retained water. S3. After micro-electrolysis of the element-retaining water, pretreated deep-sea water is obtained. This pretreated water is then concentrated to 1 / 10-1 / 2 of its original volume to obtain a deep-sea water mineral extract. In this deep-sea water mineral extract, the retention rates of calcium, magnesium, and potassium are not less than 85% of the original deep-sea water, and the retention rates of vanadium, chromium, selenium, zinc, and molybdenum are not less than 70% of the original deep-sea water.

2. The synergistic composition for promoting metabolism according to claim 1, characterized in that: The mass ratio of modified zeolite to tourmaline in the composite filter media is 3:1-5:

1. The modified zeolite is obtained by soaking natural zeolite in a 0.5-1.0 mol / L sodium chloride solution for 12-24 hours, followed by washing and drying.

3. The synergistic composition for promoting metabolism according to claim 1, characterized in that: The chelating resin is an iminodiacetic acid type or an aminophosphonic acid type chelating resin.

4. The synergistic composition for promoting metabolism according to claim 1, characterized in that: In micro-electrolysis, the DC voltage is 0.5-2.0V and the current density is 5-20A / m. 2 The processing time is 10-30 minutes.

5. The synergistic composition for promoting metabolism according to claim 1, characterized in that: 100-200 parts of deep-sea water mineral extract, 5-30 parts of synergistic components, wherein the mass ratio of polysaccharides, plant extracts and peptides is 2-5:1-3:1.5-4.

6. The synergistic composition for promoting metabolism according to claim 1, characterized in that: The polysaccharides are one or more of the following: brown algae polysaccharide sulfate, chitosan oligosaccharide, and oat β-glucan; the plant extracts are one or more of the following: bitter melon extract, mulberry leaf extract, and cinnamon extract; and the peptides are one or more of the following: corn oligopeptides, soybean peptides, and collagen peptides.

7. The application of a synergistic composition for promoting metabolism as described in any one of claims 1-6, characterized in that, The synergistic composition is used in the preparation of oral liquid beverages, solid beverages, foods or medicines that promote metabolism, help lower blood sugar and blood lipids, enhance skin cell activity, have antioxidant properties, promote collagen synthesis, improve skin moisture and elasticity, and have anti-aging functions.

8. The application of the synergistic composition for promoting metabolism according to claim 7, characterized in that, When the synergistic composition is used to prepare a liquid beverage, the specific steps include: (1) Add the synergistic components to a portion of the deep-sea mineral extract at a mass ratio of 1:1 to 3:1, and stir to dissolve at 40-60°C to form a premix; (2) Mix the premixed solution with the remaining deep-sea mineral extract, add a pH adjuster to adjust the pH to 6.5-8.0, and add an antioxidant and water; (3) Stir at room temperature for 30-60 minutes to fully complex and stabilize the mixture, then homogenize the mixture at 15-25 MPa pressure, and then sterilize to obtain the target product.

9. The application of the synergistic composition for promoting metabolism according to claim 7, characterized in that, When the synergistic composition is used to prepare a solid beverage, the specific steps include: (1) Mineral powder is prepared by spray drying of deep-sea water mineral extract, and the total mineral content in the mineral powder is ≥15%; (2) Mix the mineral powder with the synergistic components, antioxidants and pH adjusters in proportion, put them into a mixer and mix at room temperature for 30-60 minutes until uniform; (3) Package the mixed powder to obtain a solid beverage.

10. The application of the synergistic composition for promoting metabolism according to claim 7, characterized in that, When the synergistic composition is used to prepare a pharmaceutical product, the specific steps include: (1) Mineral powder is prepared by spray drying of deep-sea water mineral extract, and the total mineral content in the mineral powder is ≥15%; (2) Mix the mineral powder with the synergistic components, pH adjuster and antioxidant evenly, add purified water to granulate, dry at 40-60℃ until the moisture content is ≤5%, and granulate. (3) Add lubricant, mix well, compress into tablets to obtain tablets.

Images