A composition for turning gray hair black and its preparation method

Abstract

This invention discloses a composition for turning gray hair black. By mass percentage, the composition comprises 0.5%–8% of a fermented broth containing a traditional Chinese medicine complex, 1%–10% of a plant extract complex, 0.01%–2% of an active peptide, 0.1%–5% of a penetration and absorption-enhancing complex, 0.5%–8% of a brightening and melanin-regulating complex, 0.1%–1% of a preservative, and the balance being purified water. The active peptide is palmitate tetrapeptide-20. The preservative is selected from at least one of phenoxyethanol and ethylhexylglycerin. This invention, through the synergistic combination of traditional Chinese medicine fermentation technology with modern active peptides, plant-extracted blackening ingredients, penetration enhancers, and repairing components, achieves multiple effects including rapid gray hair blackening, gentle scalp repair, antioxidant effects, and enhanced hair follicle activity, demonstrating significant clinical and market application value.

Description

Technical Field

[0001] This application relates to the field of head care technology, specifically to a composition for turning gray hair black and its preparation method. Background Technology

[0002] Hair is an important part of the human body. It grows in hair follicles, including the hair root inside the follicle and the hair shaft that protrudes from the skin. Hair can affect a person's overall image; people with thick, black hair often look healthier and more energetic.

[0003] Currently, the proportion of young people with gray hair is increasing. With increasing social competition, young people face growing life and work pressures, coupled with irregular lifestyles and dietary habits, leading to premature graying in many young people, especially those who work night shifts or stay up late for extended periods. Gray hair not only reflects a sub-healthy state but also significantly affects appearance, giving the impression of premature aging. Besides genetic factors, gray hair is often related to psychological and mental factors. Prolonged stress or mental pressure can affect the normal functioning of the entire body, leading to insufficient blood supply to the hair follicles and difficulty in hair regrowth. In short, influenced by multiple factors such as mental stress, irregular sleep patterns, hair follicle aging, and nutritional deficiencies, the activity of melanocytes in hair follicles decreases, tyrosinase secretion is insufficient, and melanin synthesis in hair is hindered. As a result, the number of people with gray hair is increasing year by year, and the market demand for hair care products that help turn gray hair black continues to rise.

[0004] Currently, mainstream gray hair-darkening products on the market still have many technical shortcomings, making it difficult to meet the needs for safe, long-lasting, and gentle hair care. Firstly, these products generally have a long onset period, poor hair-darkening effect, and gray hair quickly reappears after discontinuation, only treating the symptoms, not the root cause. Secondly, chemical-based hair-darkening products often rely on high concentrations of oxidative chemical dyes, which are highly irritating and easily cause adverse reactions such as scalp redness and allergies, damaging the scalp barrier with long-term use. Thirdly, traditional Chinese medicine-based hair-darkening products use natural raw materials with large molecular weights, resulting in weak scalp penetration and difficulty for effective ingredients to reach the hair follicle roots, leading to fluctuating and unstable hair-darkening effects. Fourthly, most existing formulas only focus on gray hair coloring or hair follicle melanin activation, lacking a dual-care system of scalp barrier repair and hair nourishment, failing to simultaneously improve scalp sub-health and dry, damaged hair.

[0005] In conclusion, developing a gray hair reversal composition that is fast-acting, non-irritating, stable in effect, and has dual repairing effects on the scalp and hair has significant market value and clinical application significance. Summary of the Invention

[0006] To address the problems existing in current gray hair reversal products, this invention utilizes the synergistic effects of components such as traditional Chinese medicine fermentation, active peptides, nicotinamide, tetrapeptide-20, azone, HEPES, VC ethyl ether, VE, panthenol, and sodium hyaluronate to construct a four-in-one gray hair reversal system that promotes blackening, repairs, penetrates, and brightens the hair.

[0007] Specifically, the technical solution of the present invention is implemented as follows:

[0008] In a first aspect, the present invention provides a composition for turning gray hair black, wherein, by mass percentage, the composition comprises 0.5% to 8% of a fermentation broth of a traditional Chinese medicine complex, 1% to 10% of a plant extract complex, 0.01% to 2% of an active peptide, 0.1% to 5% of a penetration and absorption-promoting complex, 0.5% to 8% of a brightening and melanin-regulating complex, 0.1% to 1% of a preservative, and the balance being purified water; wherein the active peptide is palmitate tetrapeptide-20; and the preservative is selected from at least one of phenoxyethanol and ethylhexylglycerin.

[0009] In one or more embodiments, the fermentation broth of the traditional Chinese medicine complex is obtained by fermentation of the following components in parts by weight: 30-35 parts of processed Polygonum multiflorum, 10-15 parts of Eclipta prostrata, 5-9 parts of Ganoderma lucidum, 12-15 parts of Platycladus orientalis leaves, 8-10 parts of Angelica sinensis, 1-3 parts of Ligustrum lucidum, 5-8 parts of Centella asiatica, 2-4 parts of ginseng, 1-3 parts of rosemary, 1-2 parts of lavender, 2-4 parts of mulberry, 1-3 parts of raspberry, 0.5-2 parts of wolfberry, and 1-3 parts of Morinda officinalis.

[0010] Preferably, the fermentation broth of the traditional Chinese medicine complex is obtained by fermentation of the following components in parts by weight: 32.5 parts of processed Polygonum multiflorum, 13.0 parts of Eclipta prostrata, 7 parts of Ganoderma lucidum, 14 parts of Platycladus orientalis leaves, 9 parts of Angelica sinensis, 2 parts of Ligustrum lucidum, 7 parts of Centella asiatica, 3 parts of ginseng, 2 parts of rosemary, 1.5 parts of lavender, 3 parts of mulberry, 2 parts of raspberry, 1 part of wolfberry and 2 parts of Morinda officinalis.

[0011] In this invention, the traditional Chinese medicine ingredients such as processed Polygonum multiflorum, Eclipta prostrata, Ganoderma lucidum, and Platycladus orientalis leaves are bio-fermented, which can transform the large molecules of traditional Chinese medicine into absorbable small molecule active substances, greatly improving the penetration and onset speed of the active ingredients.

[0012] In one or more embodiments, the plant extract complex comprises, by weight percentage: 0.1-0.99% European larch extract, 0-0.1% tea extract, 0.1-0.99% glycine, 10-24.99% acetyltyrosine, 0-0.1% zinc chloride, 0.5% sodium metabisulfite, 50-74.99% glycerol, and 25-49.99% water.

[0013] In this invention, the plant extract complex and the fermentation liquid of the traditional Chinese medicine complex are used together to form a combination of the original Chinese medicine hair-darkening and modern protein hair-darkening, which solves the problems of slow effect of traditional Chinese medicine alone and too abrupt effect of simple scientific and technological components.

[0014] In one or more embodiments, the penetration and absorption-enhancing complex is composed of azone, HEPES, and propylene glycol in a mass ratio of 1:1:6~10.

[0015] In this invention, the penetration and absorption-enhancing complex ensures that the active ingredients can truly enter the hair papilla and hair follicle, avoiding the false effect of "only applying it to the surface".

[0016] In one or more embodiments, the brightening regulating complex is composed of nicotinamide, VC ethyl ether, vitamin E, panthenol, bismuth subtilis and sodium hyaluronate in a mass ratio of 3-6: 1-3: 1-2: 2-4: 1-3: 0.5-1.

[0017] In this invention, the brightening and regulating complex can achieve scalp barrier protection, moisturization, and anti-irritation effects, making the product more comfortable with continued use and preventing rebound. Furthermore, niacinamide can brighten and even out skin tone, and reduce oxidation at the roots of gray hair; VC ethyl ether can provide antioxidant protection and prevent gray hair regrowth; and palmitate tetrapeptide-20 can activate hair follicles and promote melanin synthesis. The combined effect of these three components achieves a complete gray hair reversal process: promoting blackening from the root, repairing shine, and stabilizing with antioxidants.

[0018] In one or more embodiments, the composition further comprises 7-8% by weight of a scalp conditioning and hair follicle activating complex.

[0019] In one or more embodiments, the scalp conditioning and hair follicle activating complex comprises, by weight percentage of the whole composition, 5.8-6.2% of Cuscuta chinensis extract, 0.4-0.6% of Saw Palmetto extract, 0.1-0.2% of Bisabolol, 0.05-0.2% of Dipotassium glycyrrhizate, 0.05-0.2% of Allantoin and 0.2-0.4% of Caffeine.

[0020] Secondly, the present invention provides a method for preparing the gray hair-turning-black composition according to any one of the above claims, comprising the following steps:

[0021] S1. Fermentation broth of traditional Chinese medicine complex: The traditional Chinese medicine components are mixed in proportion, sterilized, inoculated with bacterial strains for fermentation, and filtered to obtain the fermentation broth.

[0022] S2. Preparation of aqueous phase: Heating purified water, then adding sodium hyaluronate, nicotinamide, VC ethyl ether, HEPES and propylene glycol, stirring to dissolve, and cooling to below 50°C to obtain an aqueous phase; Optionally, adding the scalp conditioning and hair follicle activation complex to the aqueous phase and stirring to dissolve;

[0023] S3. Preparation of the active phase: Mix the plant extract complex, palmitate tetrapeptide-20, azone, vitamin E, panthenol and bisoprolol, and stir evenly to obtain the oil phase;

[0024] S4. Mixing and blending: Mix the aqueous phase and the active phase and homogenize them, then add the fermentation broth of the traditional Chinese medicine complex from step S1, and homogenize again to obtain a mixed system.

[0025] S5. Post-processing: Cool the mixture to 40°C, add the preservative, adjust the pH, stir evenly, let stand to remove bubbles, filter, and then fill to obtain the white hair to black hair composition.

[0026] In one or more embodiments, the inoculated strain in step S1 is a mixed strain composed of Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae in a mass ratio of 2:2:1.

[0027] In one or more embodiments, the pH is adjusted to 4.8 to 5.5 in step S5.

[0028] Compared with the prior art, the present invention has the following advantages:

[0029] This invention achieves multiple effects—rapidly turning gray hair black, gently repairing the scalp, providing antioxidant benefits, and enhancing hair follicle activity—through the synergistic combination of traditional Chinese medicine fermentation technology with modern active peptides, plant-extracted hair-darkening ingredients, penetration enhancers, and repairing components. It has significant clinical and market application value.

[0030] To provide a clearer understanding of this application, the specific implementation methods of this application will be described below. Detailed Implementation

[0031] To make the objectives, technical solutions, and advantages of this application clearer, the embodiments of this application will be described in further detail below.

[0032] It should be understood that the described embodiments are merely some, not all, of the embodiments of this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without creative effort are within the scope of protection of the embodiments of this application.

[0033] In the description of this application, it should be understood that the terms "first," "second," "third," etc., are used only to distinguish similar objects and are not necessarily used to describe a specific order or sequence, nor should they be construed as indicating or implying relative importance. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances. The singular forms "a," "the," and "the" used in this application and the appended claims are also intended to include the plural forms unless the context clearly indicates otherwise. The word "if" as used herein can be interpreted as "when," "when," or "in response to determination."

[0034] Furthermore, in the description of this application, unless otherwise stated, "multiple" means two or more. "And / or" describes the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A alone, A and B simultaneously, or B alone. The character " / " generally indicates that the preceding and following related objects have an "or" relationship.

[0035] Example 1: A composition A for turning gray hair black and its preparation method

[0036] A gray hair reversal composition A, by mass percentage, comprises 5% fermentation broth of traditional Chinese medicine complex, 7% plant extract complex, 1% palmitate tetrapeptide-20, 3% penetration and absorption-enhancing complex, 5% brightening and melanin-regulating complex, 0.5% preservative phenoxyethanol, and the balance being purified water.

[0037] The fermentation liquid of the traditional Chinese medicine complex, by weight, is obtained by fermentation of the following components: 32.5 parts of processed Polygonum multiflorum, 13.0 parts of Eclipta prostrata, 7 parts of Ganoderma lucidum, 14 parts of Platycladus orientalis leaves, 9 parts of Angelica sinensis, 2 parts of Ligustrum lucidum, 7 parts of Centella asiatica, 3 parts of ginseng, 2 parts of rosemary, 1.5 parts of lavender, 3 parts of mulberry, 2 parts of raspberry, 1 part of wolfberry, and 2 parts of Morinda officinalis.

[0038] The plant extract complex comprises, by weight percentage: 0.55% European larch extract, 0.05% tea extract, 0.6% glycine, 15.8% acetyltyrosine, 0.05% zinc chloride, 0.5% sodium metabisulfite, 54% glycerol, and 28.45% water.

[0039] The permeation and absorption-enhancing complex is composed of azone, HEPES and propylene glycol in a mass ratio of 1:1:8.

[0040] The brightening and regulating complex is composed of nicotinamide, VC ethyl ether, vitamin E, panthenol, bismuth subtilis and sodium hyaluronate in a mass ratio of 4:2:1.5:3:2:0.8.

[0041] The preparation method of the gray hair to black composition A includes the following steps:

[0042] S1, Fermentation broth of traditional Chinese medicine complex:

[0043] The Chinese herbal ingredients are mixed in proportion, pulverized to 80 mesh, purified water is added at a material-to-liquid ratio of 1:9, and the mixture is extracted at 80°C for 1 hour. After cooling and filtration, the extract is obtained. 2% brown sugar and 0.5% peptone are added by mass, and the mixture is then sterilized.

[0044] Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae were activated separately and cultured at 30°C for 18 hours. Then, the bacterial cultures of Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae were mixed at a mass ratio of 2:2:1 to obtain a composite seed culture.

[0045] The compound seed liquid was added to the sterilized Chinese herbal medicine components at a volume ratio of 5% for fermentation at a temperature of 30℃, a stirring speed of 150 rpm, and an aeration rate of 1:0.5 vvm. After fermentation for 48 hours, the fermentation liquid of the Chinese herbal medicine compound was obtained by filtration.

[0046] S2. Preparation of aqueous phase: Heat purified water, then add sodium hyaluronate, nicotinamide, VC ethyl ether, HEPES and propylene glycol, stir to dissolve, and cool to below 50°C to obtain the aqueous phase;

[0047] S3. Preparation of the active phase: Mix the plant extract complex, palmitate tetrapeptide-20, azone, vitamin E, panthenol and bisoprolol, and stir evenly to obtain the oil phase;

[0048] S4. Mixing and blending: Mix the aqueous phase and the active phase and homogenize them, then add the fermentation broth of the traditional Chinese medicine complex from step S1, and homogenize again to obtain a mixed system.

[0049] S5. Post-processing: Cool the mixture to 40°C, add the preservative, adjust the pH to 4.8~5.5, stir evenly, let stand to remove bubbles, filter, and then fill to obtain the white hair to black hair composition.

[0050] Example 2: A white hair-turning composition B and its preparation method

[0051] A gray hair reversal composition B, by mass percentage, comprises 0.5% fermentation broth of traditional Chinese medicine complex, 10% plant extract complex, 2% palmitate tetrapeptide-20, 5% penetration and absorption-enhancing complex, 0.5% brightening and melanin-regulating complex, 0.1% preservative, and the balance being purified water.

[0052] The fermentation broth of the traditional Chinese medicine complex, by weight, is obtained by fermentation of the following components: 35 parts of processed Polygonum multiflorum, 15 parts of Eclipta prostrata, 9 parts of Ganoderma lucidum, 15 parts of Platycladus orientalis leaves, 10 parts of Angelica sinensis, 3 parts of Ligustrum lucidum, 8 parts of Centella asiatica, 4 parts of ginseng, 3 parts of rosemary, 2 parts of lavender, 4 parts of mulberry, 3 parts of raspberry, 2 parts of wolfberry, and 3 parts of Morinda officinalis.

[0053] By weight percentage, the plant extract complex comprises: 0.1% European larch extract, 0.1% glycine, 10% acetyltyrosine, 0.5% sodium metabisulfite, 59.3% glycerol, and 30% water.

[0054] The permeation and absorption-enhancing complex is composed of azone, HEPES, and propylene glycol in a mass ratio of 1:1:6.

[0055] The brightening and regulating complex is composed of nicotinamide, VC ethyl ether, vitamin E, panthenol, bismuth subtilis and sodium hyaluronate in a mass ratio of 6:3:2:4:3:1.

[0056] The preservative is composed of phenoxyethanol and ethylhexylglycerin in a mass ratio of 1:1.

[0057] The preparation method of the gray hair to black composition B includes the following steps:

[0058] S1, Fermentation broth of traditional Chinese medicine complex:

[0059] The Chinese herbal ingredients are mixed in proportion, pulverized to 80 mesh, purified water is added at a material-to-liquid ratio of 1:10, and the mixture is extracted at 80°C for 1 hour. After cooling and filtration, the extract is obtained. 2% brown sugar and 0.5% peptone are added by mass, and the mixture is then sterilized.

[0060] Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae were activated separately and cultured at 30°C for 18 hours. Then, the bacterial cultures of Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae were mixed at a mass ratio of 2:2:1 to obtain a composite seed culture.

[0061] The compound seed liquid was added to the sterilized Chinese herbal medicine components at a volume ratio of 5% for fermentation at a temperature of 30℃, a stirring speed of 150 rpm, and an aeration rate of 1:0.5 vvm. After fermentation for 48 hours, the fermentation liquid of the Chinese herbal medicine compound was obtained by filtration.

[0062] S2. Preparation of aqueous phase: Heat purified water, then add sodium hyaluronate, nicotinamide, VC ethyl ether, HEPES and propylene glycol, stir to dissolve, and cool to below 50°C to obtain the aqueous phase;

[0063] S3. Preparation of the active phase: Mix the plant extract complex, palmitate tetrapeptide-20, azone, vitamin E, panthenol and bisoprolol, and stir evenly to obtain the oil phase;

[0064] S4. Mixing and blending: Mix the aqueous phase and the active phase and homogenize them, then add the fermentation broth of the traditional Chinese medicine complex from step S1, and homogenize again to obtain a mixed system.

[0065] S5. Post-processing: Cool the mixture to 40°C, add the preservative, adjust the pH to 4.8~5.5, stir evenly, let stand to remove bubbles, filter, and then fill to obtain the white hair to black hair composition.

[0066] Example 3: A white hair to blackening composition C and its preparation method

[0067] A gray hair reversal composition C, by mass percentage, comprises 8% fermentation broth of traditional Chinese medicine complex, 1% plant extract complex, 0.01% palmitate tetrapeptide-20, 0.1% penetration and absorption-enhancing complex, 8% brightening and melanin-regulating complex, 1% preservative ethylhexylglycerin, and the balance being purified water.

[0068] The fermentation broth of the traditional Chinese medicine complex, by weight, is obtained by fermentation of the following components: 30-35 parts of processed Polygonum multiflorum, 10 parts of Eclipta prostrata, 5 parts of Ganoderma lucidum, 12 parts of Platycladus orientalis leaves, 8 parts of Angelica sinensis, 1 part of Ligustrum lucidum, 5 parts of Centella asiatica, 2 parts of ginseng, 1 part of rosemary, 1 part of lavender, 2 parts of mulberry, 1 part of raspberry, 0.5 parts of wolfberry, and 1 part of Morinda officinalis.

[0069] The plant extract complex comprises, by weight percentage: 0.99% European larch extract, 0.1% tea extract, 0.99% glycine, 22.32% acetyltyrosine, 0.1% zinc chloride, 0.5% sodium metabisulfite, 50% glycerol, and 25% water.

[0070] The penetration and absorption-enhancing complex is composed of azone, HEPES, and propylene glycol in a mass ratio of 1:1:10.

[0071] The brightening and regulating complex is composed of nicotinamide, VC ethyl ether, vitamin E, panthenol, bismuth subtilis and sodium hyaluronate in a mass ratio of 3:1:1:2:1:0.5.

[0072] The preparation method of the gray hair to black composition C includes the following steps:

[0073] S1, Fermentation broth of traditional Chinese medicine complex:

[0074] The Chinese herbal ingredients are mixed in proportion, pulverized to 80 mesh, purified water is added at a material-to-liquid ratio of 1:8, and the mixture is extracted at 80°C for 1 hour. After cooling and filtration, the extract is obtained. 2% brown sugar and 0.5% peptone are added by mass, and the mixture is then sterilized.

[0075] Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae were activated separately and cultured at 30°C for 18 hours. Then, the bacterial cultures of Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae were mixed at a mass ratio of 2:2:1 to obtain a composite seed culture.

[0076] The compound seed liquid was added to the sterilized Chinese herbal medicine components at a volume ratio of 5% for fermentation at a temperature of 30℃, a stirring speed of 150 rpm, and an aeration rate of 1:0.5 vvm. After fermentation for 48 hours, the fermentation liquid of the Chinese herbal medicine compound was obtained by filtration.

[0077] S2. Preparation of aqueous phase: Heat purified water, then add sodium hyaluronate, nicotinamide, VC ethyl ether, HEPES and propylene glycol, stir to dissolve, and cool to below 50°C to obtain the aqueous phase;

[0078] S3. Preparation of the active phase: Mix the plant extract complex, palmitate tetrapeptide-20, azone, vitamin E, panthenol and bisoprolol, and stir evenly to obtain the oil phase;

[0079] S4. Mixing and blending: Mix the aqueous phase and the active phase and homogenize them, then add the fermentation broth of the traditional Chinese medicine complex from step S1, and homogenize again to obtain a mixed system.

[0080] S5. Post-processing: Cool the mixture to 40°C, add the preservative, adjust the pH to 4.8~5.5, stir evenly, let stand to remove bubbles, filter, and then fill to obtain the white hair to black hair composition.

[0081] Example 4: A white hair to blackening composition D and its preparation method

[0082] A gray hair reversal composition D, which has a similar composition to composition A in Example 1, except that, by weight percentage, composition D further contains 7.15% of a scalp conditioning and hair follicle activation complex.

[0083] Specifically, based on the total mass percentage of the composition, the scalp conditioning and hair follicle activation complex consists of 6% Cuscuta chinensis extract, 0.5% Saw palmetto extract, 0.15% Bisabolol, 0.1% Dipotassium glycyrrhizate, 0.1% Allantoin and 0.3% Caffeine.

[0084] The preparation method of the gray hair to black composition D is basically the same as the preparation method of composition A in Example 1, except that 7.15% of the above-mentioned scalp conditioning and hair follicle activation complex is added to the aqueous phase in step S2.

[0085] Comparative Example 1: A composition E for turning gray hair black and its preparation method

[0086] A white hair turning black composition E differs from Example 1 in that it does not contain the fermentation broth of the traditional Chinese medicine complex, but uses purified water instead; the remaining components are the same as those of the white hair turning black composition A described in Example 1.

[0087] Compared with Example 1, the preparation method described herein does not include the preparation step of the fermentation broth of the traditional Chinese medicine complex; otherwise, it is the same as Example 1.

[0088] Comparative Example 2: A composition F for turning gray hair black and its preparation method

[0089] A gray hair reversal composition F differs from Example 2 in that it does not contain a plant extract complex, but uses purified water instead; the remaining components are the same as those of the gray hair reversal composition B described in Example 2.

[0090] The preparation method described herein is the same as that in Example 2, except that no components of the plant extract complex are added.

[0091] Comparative Example 3: A gray hair-turning composition G and its preparation method

[0092] A gray hair to blackening composition G differs from Example 3 in that it does not contain palmitate tetrapeptide-20, but uses purified water instead; the remaining components are the same as those in the gray hair to blackening composition C described in Example 3.

[0093] The preparation method described herein is the same as that in Example 3, except that palmitate tetrapeptide-20 is not added.

[0094] Comparative Example 4: A composition H for turning gray hair black and its preparation method

[0095] A gray hair to blackening composition H differs from Example 1 in that it does not contain the penetration and absorption-enhancing complex, and uses purified water instead; the remaining components are the same as those of the gray hair to blackening composition A described in Example 1.

[0096] The preparation method described herein is the same as that in Example 1, except that no permeation and absorption-enhancing complex components are added.

[0097] Comparative Example 5: A composition I for turning gray hair black and its preparation method

[0098] A gray hair to blackening composition I differs from Example 1 in that it does not contain the brightening and melanin-regulating complex, and uses purified water instead; the remaining components are the same as those in the gray hair to blackening composition A described in Example 1.

[0099] The preparation method described herein is the same as that in Example 1, except that no brightening and melanin regulation complex is added.

[0100] Case 1: Clinical Trial

[0101] Subjects: 120 patients with gray hair were randomly selected, including 60 males and 60 females, with the youngest being 21 years old, the oldest being 60 years old, and the average age being 33 years old. The 120 patients with gray hair were randomly divided into 8 groups of 15 patients each, corresponding to compositions AD prepared in Examples 1-3 and compositions EI prepared in Comparative Examples 1-5, respectively.

[0102] Experimental method: Each day, after washing and massaging the hair of each group of gray hair patients, the corresponding composition was applied directly to the scalp to allow it to penetrate into the hair follicles. This was repeated for one month. The results are shown in Table 1.

[0103] Criteria for judging therapeutic efficacy:

[0104] Effective: Gray hair turns black; Improved: Gray hair gradually turns gray; Ineffective: Gray hair shows no improvement;

[0105] Table 1. Results of the experiment on gray hair turning black in each group

[0106] Example 1 15 0 0 Example 2 15 0 0 Example 3 14 1 0 Example 4 15 0 0 Comparative Example 1 2 6 7 Comparative Example 2 5 5 5 Comparative Example 3 3 10 2 Comparative Example 4 8 6 1 Comparative Example 5 7 8 0

[0107] The above results show that the compositions prepared in Examples 1-4 are highly effective, achieving almost complete blackening of gray hair; while the compositions prepared in Comparative Examples 1-5 are less effective, with only a small number of patients achieving blackening of gray hair. Therefore, the gray hair blackening composition of this invention has a synergistic effect among its components, constructing a four-in-one gray hair blackening system of "promoting blackening + repairing + penetrating + brightening".

[0108] It should also be noted that the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus. Unless otherwise specified, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes that element.

[0109] The above are merely embodiments of this application and are not intended to limit the scope of this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the scope of the claims of this application.

Claims

1. A composition for turning gray hair black, characterized in that, By weight percentage, the composition comprises 0.5% to 8% fermentation broth of traditional Chinese medicine complex, 1% to 10% plant extract complex, 0.01% to 2% active peptide, 0.1% to 5% penetration and absorption-enhancing complex, 0.5% to 8% brightening and melanin-regulating complex, 0.1% to 1% preservative, and the balance being purified water; the active peptide is palmitate tetrapeptide-20; the preservative is selected from at least one of phenoxyethanol and ethylhexylglycerin.

2. The hair-turning-black composition according to claim 1, characterized in that, The fermentation broth of the traditional Chinese medicine complex, by weight, is obtained by fermentation of the following components: 30-35 parts of processed Polygonum multiflorum, 10-15 parts of Eclipta prostrata, 5-9 parts of Ganoderma lucidum, 12-15 parts of Platycladus orientalis leaves, 8-10 parts of Angelica sinensis, 1-3 parts of Ligustrum lucidum, 5-8 parts of Centella asiatica, 2-4 parts of ginseng, 1-3 parts of rosemary, 1-2 parts of lavender, 2-4 parts of mulberry, 1-3 parts of raspberry, 0.5-2 parts of wolfberry, and 1-3 parts of Morinda officinalis.

3. The hair-turning-black composition according to claim 2, characterized in that, The fermentation broth of the traditional Chinese medicine complex, by weight, is obtained by fermentation of the following components: 32.5 parts of processed Polygonum multiflorum, 13.0 parts of Eclipta prostrata, 7 parts of Ganoderma lucidum, 14 parts of Platycladus orientalis leaves, 9 parts of Angelica sinensis, 2 parts of Ligustrum lucidum, 7 parts of Centella asiatica, 3 parts of ginseng, 2 parts of rosemary, 1.5 parts of lavender, 3 parts of mulberry, 2 parts of raspberry, 1 part of wolfberry, and 2 parts of Morinda officinalis.

4. The hair-turning-black composition according to claim 1, characterized in that, The plant extract complex comprises, by weight percentage: 0.1-0.99% European larch extract, 0-0.1% tea extract, 0.1-0.99% glycine, 10-24.99% acetyltyrosine, 0-0.1% zinc chloride, 0.5% sodium metabisulfite, 50-74.99% glycerol, and 25-49.99% water.

5. The hair-turning-black composition according to claim 1, characterized in that, The penetration and absorption-enhancing complex is composed of azone, HEPES, and propylene glycol in a mass ratio of 1:1:6~10.

6. The hair-turning composition according to claim 1, characterized in that, The brightening and regulating complex is composed of nicotinamide, VC ethyl ether, vitamin E, panthenol, bismuth subtilis and sodium hyaluronate in a mass ratio of 3~6 : 1~3 : 1~2 : 2~4 : 1~3 : 0.5~1.

7. The hair-turning composition according to claim 1, characterized in that, The composition further comprises 7-8% of a scalp conditioning and hair follicle activating complex by weight percentage. Based on the total mass percentage of the composition, the scalp conditioning and hair follicle activation complex consists of 5.8-6.2% Cuscuta chinensis extract, 0.4-0.6% Saw palmetto extract, 0.1-0.2% Bisabolol, 0.05-0.2% Dipotassium glycyrrhizate, 0.05-0.2% Allantoin and 0.2-0.4% Caffeine.

8. A method for preparing the gray hair-turning composition according to any one of claims 1 to 7, characterized in that, Includes the following steps: S1. Fermentation broth of traditional Chinese medicine complex: The traditional Chinese medicine components are mixed in proportion, sterilized, inoculated with bacterial strains for fermentation, and filtered to obtain the fermentation broth. S2. Preparation of aqueous phase: Heating purified water, then adding sodium hyaluronate, nicotinamide, VC ethyl ether, HEPES and propylene glycol, stirring to dissolve, and cooling to below 50°C to obtain an aqueous phase; Optionally, adding the scalp conditioning and hair follicle activation complex to the aqueous phase and stirring to dissolve; S3. Preparation of the active phase: Mix the plant extract complex, palmitate tetrapeptide-20, azone, vitamin E, panthenol and bisoprolol, and stir evenly to obtain the oil phase; S4. Mixing and blending: Mix the aqueous phase and the active phase and homogenize them, then add the fermentation broth of the traditional Chinese medicine complex from step S1, and homogenize again to obtain a mixed system. S5. Post-processing: Cool the mixture to 40°C, add the preservative, adjust the pH, stir evenly, let stand to remove bubbles, filter, and then fill to obtain the white hair to black hair composition.

9. The preparation method according to claim 1, characterized in that, The inoculated strain in step S1 is a mixed strain composed of Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae in a mass ratio of 2:2:

1.

10. The preparation method according to claim 1, characterized in that, In step S5, the pH is adjusted to 4.8~5.5.