A pipette tip box for immunohistochemical staining

Abstract

This invention discloses a pipette tip box for immunohistochemical staining, belonging to the field of biological experimental consumables technology. The tip box includes a base, a fixing frame, a placement box, a moving adjustment mechanism, and a partition adjustment mechanism. The moving adjustment mechanism includes a first threaded rod and a moving plate, used to drive the mounting base to move out of or into the base, facilitating the installation and replacement of the cold storage module. The partition adjustment mechanism includes a second threaded rod and a first moving block, used to drive the partition plate to move within the placement box, dynamically dividing the internal space of the placement box into multiple independent areas. This invention, by integrating a removable cold storage module mounting structure and a dynamically adjustable internal area partitioning structure, effectively solves the problems of existing pipette tip boxes being unable to manage different reagent tips in separate areas and unable to maintain a low-temperature environment, reducing the risk of cross-contamination in immunohistochemical staining experiments and improving the convenience of operation and the reliability of experimental results.

Description

Technical Field

[0001] This invention relates to the field of biological experimental consumables technology, specifically to a pipette tip box for immunohistochemical staining. Background Technology

[0002] Immunohistochemical staining is a core technique in biological and medical research. Based on the principle of specific antigen-antibody binding, it detects target proteins in tissue or cell sections through a colorimetric reaction. The experimental procedure is complex, involving various reagents, including but not limited to primary antibodies, secondary antibodies, blocking solutions, washing solutions, and counterstaining solutions. Pipette tips are essential consumables in the aspiration and dispensing of these reagents, and tip cases are used to store and protect these pipette tips.

[0003] A significant technical bottleneck exists in current immunohistochemical staining procedures: operators often need to take pipette tips from the same tip box to aspirate multiple different reagents. This practice easily leads to cross-contamination between different reagents. For example, a pipette tip that has been used to aspirate the primary antibody may come into contact with a reagent bottle again, or residue may be introduced into another reagent. This can seriously interfere with the accuracy of staining results, producing false positives or false negatives.

[0004] To address cross-contamination, operators sometimes frequently change pipette tip boxes from different batches, opening a new box for each reagent drawn. However, this not only results in significant waste of consumables and increases experimental costs, but more importantly, the frequent opening, taking, and closing of boxes greatly slows down the experimental process and reduces work efficiency, especially in high-throughput staining experiments where this effect is even more pronounced.

[0005] Furthermore, many antibodies and reagents used in immunohistochemistry experiments are temperature-sensitive and need to be stored at low temperatures (e.g., 2-8°C) to maintain their activity. However, traditional pipette tip boxes typically lack insulation or cold storage capabilities. During prolonged operation at room temperature, the pipette tips themselves may absorb heat from the environment. When drawing low-temperature reagents, the temperature difference between the inside and outside of the tip may cause changes in reagent properties or the formation of bubbles, affecting sample loading accuracy. Even if the entire pipette tip box is refrigerated, it will rapidly heat up upon removal, failing to provide continuous low-temperature protection during operation.

[0006] Therefore, there is an urgent need for a new type of pipette tip holder that can effectively manage pipette tips for different purposes without significantly increasing the number of operation steps, avoid cross-contamination, and provide continuous low-temperature protection for the pipette tips during experimental operations, so as to solve the above-mentioned problems in the existing technology. Summary of the Invention

[0007] Therefore, the present invention provides a pipette tip box for immunohistochemical staining to solve the above-mentioned problems in the prior art.

[0008] To achieve the above objectives, the present invention provides the following technical solution:

[0009] According to a first aspect of the present invention, a pipette tip box and base for immunohistochemical staining;

[0010] A fixed frame is fixed to the top of the base;

[0011] A placement box, which is fixed to the top of the fixed frame, is used to accommodate pipette tips;

[0012] A movable adjustment mechanism includes a first threaded rod and a movable plate. The first threaded rod is rotatably connected to the interior of the fixed frame, and the movable plate is threadedly connected to the first threaded rod. A mounting base for supporting the cold storage module is fixedly provided on the movable plate.

[0013] The partition adjustment mechanism includes a second threaded rod and a first movable block. The second threaded rod is rotatably connected to the inside of the placement box, and the first movable block is threadedly connected to the second threaded rod. A partition plate is fixed on the first movable block.

[0014] The movable plate is configured to move the mounting base out of or into the area of ​​the base when the first threaded rod rotates; the baffle plate is configured to move within the placement box when the second threaded rod rotates, so as to adjust the internal space layout of the placement box.

[0015] Furthermore, a cold storage module is movably connected to one side of the mounting base. This detachable connection (such as a snap-fit, magnetic attraction, or sliding groove) allows for easy replacement and pre-cooling of the cold storage module.

[0016] Furthermore, a protective cover is rotatably connected to one side of the top of the placement box. The protective cover prevents external dust from falling in and maintains a stable internal environment when the gun tip is used.

[0017] Furthermore, the movable adjustment mechanism also includes: a first gear fixed to the first threaded rod; a second gear rotatably connected to the fixed frame and meshing with the first gear; and a first rotating handle, one end of which is fixed to the second gear and the other end of which extends outside the fixed frame. This gear transmission structure provides an efficient and labor-saving driving method, and the handle extending out of the fixed frame facilitates operation by the operator from the outside.

[0018] Furthermore, the partition adjustment mechanism also includes: a third gear fixed to the second threaded rod; a fourth gear rotatably connected inside the placement box and meshing with the third gear; and a second rotating handle, one end of which is fixed to the fourth gear and the other end of which extends outside the placement box. Similarly, this gear transmission structure makes the adjustment of the partition more precise and convenient.

[0019] Furthermore, a first sliding rod is fixed inside the fixed frame, and one end of the movable plate is slidably connected to the first sliding rod. The sliding rod provides guidance and support for the translation of the movable plate, ensuring the smoothness of the mounting base's movement in / out and preventing it from deflecting or getting stuck.

[0020] Furthermore, a second sliding rod is fixedly installed inside the placement box, and the partition adjustment mechanism also includes a second moving block. The second moving block is slidably connected to the second sliding rod and is fixedly connected to the partition plate. The second sliding rod and the second moving block cooperate to provide a second parallel guide path for the movement of the partition plate, significantly improving the stability and anti-tilting ability of the partition plate movement.

[0021] Furthermore, the protective cap is equipped with a push-button spring mechanism to enable quick opening and closing with one hand. This user-friendly design allows operators to quickly open the protective cap and access the pipette tip with one hand, while the other hand continues to operate the pipette, improving the smoothness of the operation.

[0022] Furthermore, there are multiple baffles arranged side by side to divide the internal space of the placement box into multiple parallel, independent pipette tip placement areas. This allows for the simultaneous creation of multiple independent partitions, suitable for complex multi-antibody experimental workflows.

[0023] Furthermore, each of the independent nozzle placement areas is provided with at least one vent hole at its bottom. The design of the vent hole can balance the air pressure inside and outside the partition and inside and outside the nozzle box, so that no "suction" will be generated due to the air pressure difference when taking the nozzle, ensuring smooth use; at the same time, in low temperature environment, the vent hole can also prevent excessive accumulation of condensate.

[0024] This invention has the following advantages: In the technical solution of this invention, by setting a movable plate structure driven by a threaded rod, the mounting base for placing the cold storage module can be actively moved out from inside the base. This greatly facilitates the replacement, installation, or maintenance of the cold storage module by the operator. For example, before the experiment begins, the operator can conveniently place the pre-cooled cold storage module (such as an ice pack, phase change material cold storage box, etc.) on the moved mounting base, and then, by rotating the threaded rod in the opposite direction, smoothly and accurately store the cold storage module inside the base, making it fit tightly against the bottom of the placement box, thereby providing a continuous low-temperature environment for the pipette tips inside the placement box. This effectively avoids the inconvenience of needing to invert or tilt the entire pipette tip box to install the cold storage module in traditional designs, and also ensures the uniformity of the cold storage effect.

[0025] Meanwhile, this invention integrates a baffle structure driven by a second threaded rod inside the placement box. By rotating the second threaded rod, the linear movement of the first moving block and its baffle within the placement box can be precisely controlled. This allows operators to dynamically divide the internal space of the placement box into multiple adjustable and independent small areas according to the actual needs of the experiment. For example, when performing staining experiments with multiple primary antibodies, pipette tips dedicated to each antibody can be placed in different baffle areas, thus achieving strict physical isolation and fundamentally eliminating cross-contamination of different reagents caused by using the same box of pipette tips. When partitioning is not required, the baffles can be moved to one side, restoring the overall space of the placement box. This flexible and controllable partitioning management method balances contamination prevention and space utilization.

[0026] This invention solves two core pain points in immunohistochemical staining experiments—low temperature maintenance and cross-contamination control—through the synergistic effect of a "removable cold storage module structure" and a "dynamically adjustable partition structure," greatly improving the convenience, reliability, and efficiency of the experiment. Attached Figure Description

[0027] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings in the following description are merely exemplary, and those skilled in the art can derive other embodiments based on the provided drawings without creative effort.

[0028] The structures, proportions, sizes, etc. illustrated in this specification are only for the purpose of assisting those skilled in the art in understanding and reading the content disclosed herein, and are not intended to limit the conditions under which the present invention can be implemented. Therefore, they have no substantial technical significance. Any modifications to the structure, changes in the proportions, or adjustments to the size, without affecting the effects and objectives that the present invention can produce, should still fall within the scope of the technical content disclosed in the present invention.

[0029] Figure 1 This is a three-dimensional structural schematic diagram of a pipette tip box for immunohistochemical staining provided in some embodiments of the present invention.

[0030] Figure 2 This is a side view of a pipette tip box for immunohistochemical staining provided in some embodiments of the present invention.

[0031] Figure 3 This is a cross-sectional view of a placement box for an immunohistochemical staining pipette tip holder, provided in some embodiments of the present invention.

[0032] Figure 4 This is a cross-sectional view of the fixing frame of a pipette tip box for immunohistochemical staining, provided in some embodiments of the present invention.

[0033] In the diagram: 1. Base; 2. Fixed frame; 3. First threaded rod; 4. Moving plate; 5. Mounting seat; 6. Placement box; 7. First groove; 8. Second threaded rod; 9. First moving block; 10. Baffle plate; 11. Cold storage module; 12. Protective cover; 13. First gear; 14. Second gear; 15. Third gear; 16. Fourth gear; 17. First rotating handle; 18. Second rotating handle; 19. First sliding rod; 20. Second sliding rod; 21. Second moving block. Detailed Implementation

[0034] The following specific embodiments illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0035] Example 1

[0036] like Figures 1 to 4As shown, an immunohistochemical staining pipette tip box according to a first aspect embodiment of the present invention includes a generally flat base 1, which serves as the supporting foundation for the entire device. Two vertically arranged fixing frames 2 are symmetrically fixed to one side of the top of the base 1 by means of welding, bolting, or integral molding. These two fixing frames 2 are parallel to each other and spaced at a predetermined distance, forming a receiving space.

[0037] Between the two fixed frames 2, a movable adjustment mechanism is provided. Specifically, a first threaded rod 3 is rotatably connected between the two sides of the interior of one fixed frame 2 via bearings. The first threaded rod 3 extends horizontally. On the outside of the first threaded rod 3, a movable plate 4 is connected via a threaded engagement. One end of the movable plate 4 has an internal threaded hole, forming a screw-nut pair with the first threaded rod 3. The other end of the movable plate 4 extends toward the other fixed frame 2. On the side of the movable plate 4 facing the outside of the base 1, a mounting base 5 is fixedly connected. The mounting base 5 can be a platform, a slot, or a frame structure, the specific shape and size of which match the cold storage module 11 to be installed.

[0038] At the top of the two fixed frames 2, a placement box 6 is fixedly connected. The placement box 6 is a box-shaped structure with an open top, and its internal space is used to neatly arrange and accommodate multiple pipette tips. The bottom of the placement box 6 is close to the base 1.

[0039] Furthermore, an adjustable partition mechanism is integrated inside the placement box 6. Specifically, first grooves 7 are symmetrically formed on two opposite side walls inside the placement box 6. A second threaded rod 8 is rotatably connected between the two sides of the interior of one of the first grooves 7 via a bearing. The second threaded rod 8 also extends horizontally, but its extension direction can be the same as or perpendicular to the first threaded rod 3. In a preferred layout, the two are perpendicular to each other to achieve different degrees of adjustment freedom. On the outside of the second threaded rod 8, a first moving block 9 is connected via a threaded engagement. The first moving block 9 and the second threaded rod 8 form another screw-nut pair. On the side of the first moving block 9 facing the interior of the placement box 6, a partition plate 10 is fixedly connected. The dimensions of the partition plate 10 match the internal height and width of the placement box 6, enabling it to effectively divide the internal space of the placement box 6 into two.

[0040] The working principle of this embodiment is as follows:

[0041] When a low-temperature environment is required for the nozzle box, the operator first rotates the first threaded rod 3. The rotational motion of the first threaded rod 3 is converted into the linear motion of the moving plate 4 through the threaded joint. The moving plate 4 drives the mounting seat 5 on it to move smoothly outward from a storage position located inside the base 1 (i.e., directly below the box 6) until it is completely removed from the outer area of ​​the base 1.

[0042] At this point, the operator can easily place the pre-cooled cold storage module 11 (e.g., a packaged cold storage gel pack or a metal cold storage plate) onto the mounting base 5 and fix it in place by snap-fit, magnetic attraction or simple interlocking structure.

[0043] Subsequently, the operator rotates the first threaded rod 3 in the reverse direction, causing the moving plate 4 to move the mounting base 5 and its cold storage module 11 in the opposite direction, retracting them back into the base 1. At this point, the cold storage module 11 is positioned directly below the placement box 6, with the two in close contact or with only a small gap. The cold energy stored in the cold storage module 11 is transferred to the placement box 6 through heat conduction and radiation, thereby lowering the temperature inside the placement box 6 and the pipette tips it contains, creating a continuous low-temperature microenvironment. This is particularly important for pipette tips used with antibodies or enzyme reagents that require cryopreservation.

[0044] When multi-reagent staining experiments are required to avoid cross-contamination, the operator rotates the second threaded rod 8. The rotation of the second threaded rod 8 drives the first moving block 9 to move linearly along its axis. The first moving block 9 causes the partition plate 10, which is fixed to it, to move smoothly inside the placement box 6. As needed, the operator can move the partition plate 10 to a predetermined position inside the placement box 6, for example, dividing the placement box 6 into left and right areas. Then, the operator can place a dedicated pipette tip for drawing primary antibodies in the left area and a pipette tip for drawing secondary antibodies or washing solution in the right area. Due to the physical isolation provided by the partition plate 10, any minor splashes or unintentional contact when using the left pipette tip will not contaminate the pipette tip in the right area, and vice versa.

[0045] When the experiment is over or no partitioning is needed, the second threaded rod 8 can be rotated again to move the partition 10 to the far side of the placement box 6 so that it does not occupy effective space, and the placement box 6 returns to a whole space.

[0046] Example 2

[0047] like Figures 1 to 4 As shown in this embodiment, another pipette tip box for immunohistochemical staining has the same structure as in Example 1. Only the different parts are described below.

[0048] In this embodiment, a guide structure is added to improve the stability and accuracy of movement.

[0049] Please see Figure 4 Between the two sides inside a fixed frame 2, in addition to the first threaded rod 3, a first sliding rod 19 is also fixedly connected. The first sliding rod 19 is arranged parallel to the first threaded rod 3. A smooth through hole is provided on the movable plate 4, which is slidably fitted onto the outside of the first sliding rod 19.

[0050] When the first threaded rod 3 drives the moving plate 4 to move, the other end of the moving plate 4 (the end not connected to the first threaded rod 3) simultaneously slides along the first sliding rod 19. This "one rod driving, one rod guiding" double-rod structure effectively prevents the moving plate 4 from rotating or tilting laterally around the axis of the first threaded rod 3 during movement, ensuring that the mounting base 5 and its cold storage module 11 can always smoothly enter and exit the base 1 along a precise straight path, avoiding scratching or jamming with the side wall of the base 1 due to shaking or misalignment.

[0051] Please see Figure 3 Similarly, within the first groove 7, between the two sides, in addition to the second threaded rod 8, a second sliding rod 20 is fixedly connected. The second sliding rod 20 is arranged parallel to the second threaded rod 8. The partition adjustment mechanism also includes a second moving block 21. The second moving block 21 has a smooth through hole for sliding connection to the outside of the second sliding rod 20, and one side of the second moving block 21 is also fixedly connected to the partition plate 10.

[0052] In this embodiment, the partition 10 is simultaneously connected to the first moving block 9 (driven by the second threaded rod 8) and the second moving block 21 (guided by the second sliding rod 20). When the second threaded rod 8 rotates, the first moving block 9 and the second moving block 21 move synchronously on the second threaded rod 8 and the second sliding rod 20, respectively, jointly driving the partition 10 to move. This dual-point connection and guiding method significantly enhances the rigidity of the partition 10 and avoids its tilting due to uneven force during movement, ensuring that the partition 10 always remains perpendicular to the side wall of the placement box 6, thereby forming a stable and reliable partitioning isolation effect.

[0053] The other structures, connections, and working principles of this embodiment are the same as those of Embodiment 1, and will not be repeated here.

[0054] Example 3

[0055] like Figures 1 to 4 As shown in this embodiment, another pipette tip box for immunohistochemical staining has the same structure as in Embodiment 2. Only the different parts are described below.

[0056] In this embodiment, a gear transmission mechanism is added to provide a more effortless and ergonomic operating method.

[0057] Please see Figure 4Regarding the movable adjustment mechanism: A first gear 13 is coaxially fixedly connected to the outside of the first threaded rod 3. Simultaneously, a second gear 14 is rotatably connected to the inside of the same fixed frame 2 via a rotating shaft. The first gear 13 and the second gear 14 are configured to mesh with each other. The gear ratio between them can be designed according to the required torque and transmission speed. For example, the second gear 14 can be set to have more teeth than the first gear 13 to achieve the purpose of saving effort. The rotating shaft of the second gear 14 extends outward and is fixedly connected to a first rotating handle 17. The other end of the first rotating handle 17 passes through a hole opened in the side wall of the fixed frame 2 and extends to the outside of the fixed frame 2 for easy gripping by the operator.

[0058] When the operator rotates the first rotary handle 17, the second gear 14 rotates accordingly. Through gear meshing, the second gear 14 drives the first gear 13 to rotate, which in turn drives the first threaded rod 3, which is coaxial with it, to rotate. This gear transmission system efficiently transmits the rotational motion and torque applied to the handle by the operator to the first threaded rod 3, and the feel can be adjusted by changing the gear diameter ratio.

[0059] Please see Figure 3 Regarding the partition adjustment mechanism: Similarly, a third gear 15 is coaxially fixedly connected to the outside of the second threaded rod 8. Inside the same first groove 7, a fourth gear 16 is rotatably connected via a rotating shaft. The third gear 15 and the fourth gear 16 mesh with each other. The rotating shaft of the fourth gear 16 extends outward and is fixedly connected to a second rotating handle 18. The second rotating handle 18 passes through an opening in the side wall of the placement box 6 and extends to the outside of the placement box 6.

[0060] When the operator rotates the second rotating handle 18, the fourth gear 16 rotates, driving the third gear 15, which meshes with it, to rotate, thereby causing the second threaded rod 8 to rotate. Similarly, by setting the size of the fourth gear 16 to be larger than that of the third gear 15, a labor-saving effect can be achieved, which is particularly useful for application scenarios that require frequent movement of the baffle 10.

[0061] In this embodiment, by extending the operating handle to the outside of the device and using a mature gear transmission, the operator can easily and accurately control the loading and unloading of the cold storage module and the division of the internal area with just one hand without any tools, which greatly improves the user experience and operating efficiency.

[0062] Example 4

[0063] like Figures 1 to 4 As shown in this embodiment, another pipette tip box for immunohistochemical staining has the same structure as all of Examples 1 to 3. Only the different parts are described below.

[0064] In this embodiment, a protective cover and its quick-opening and closing structure are added. A protective cover 12 is rotatably connected to the top edge of the placement box 6 via a hinge or hinge. The shape and size of the protective cover 12 match the top opening of the placement box 6. When closed, it can completely cover the placement box 6, effectively preventing external dust, aerosols and other contaminants from entering.

[0065] Furthermore, to achieve quick and convenient operation, the protective cover 12 integrates a press-type spring-loaded structure. This structure is a mature existing technology, commonly found in optical drives, cabinet doors, etc. Its basic principle is as follows: a locking mechanism with a spring and a latch is set on the closed contact surface between the protective cover 12 and the housing 6. When the protective cover 12 is gently pressed down, the locking mechanism automatically engages, maintaining the closed state. When it needs to be opened, simply press the protective cover 12 again, and the locking mechanism will release the spring's potential energy, automatically springing the protective cover 12 upwards at an angle, placing it in a slightly open state, allowing the operator to easily lift it completely.

[0066] In this embodiment, combined with a press-type spring-loaded structure, when performing sample application, the operator can lightly press the protective cap 12 with one hand (usually the hand holding the pipette) at the wrist or elbow, and the protective cap 12 will automatically spring open, allowing direct access to the pipette tip. Throughout the process, the other hand does not need to participate in the cap-opening action, allowing for a stable grip on the pipette or sample tube, greatly simplifying the operation process and improving work efficiency in fast-paced experiments.

[0067] Example 5

[0068] like Figures 1 to 4 As shown in the figure, another pipette tip box for immunohistochemical staining provided in this embodiment has the same structure as in embodiment 4. Only the different parts are described below.

[0069] In this embodiment, the partition adjustment mechanism has been extended.

[0070] Unlike the previous embodiments which only had one partition 10, this embodiment has two or more partitions 10 arranged side by side inside the placement box 6. Correspondingly, each partition 10 is independently connected to a corresponding set of first moving blocks 9 and second moving blocks 21. Simultaneously, each set of moving blocks can be driven by an independent second threaded rod 8, or, more efficiently, multiple threads with the same or opposite directions can be machined on the same second threaded rod 8 to simultaneously drive multiple moving blocks to move in opposite directions.

[0071] For example, two partitions 10 are installed inside the placement box 6, which can divide the entire space into three independent areas. When performing a staining experiment containing three different primary antibodies, the operator can label these three areas as "Primary Antibody A Dedicated Pipeline Tip Area," "Primary Antibody B Dedicated Pipeline Tip Area," and "Universal Wash Solution Pipeline Tip Area," respectively. This precise physical partitioning eliminates any possibility of cross-contamination at the source, making it possible to safely handle multiple antibodies in the same batch of experiments.

[0072] To further enhance the usability of individual areas, one or more vents can be provided at the bottom of each independent nozzle placement area. These vents are small in diameter, insufficient for the nozzle tip to drop, but large enough to balance the air pressure. When used in low-temperature environments, the air pressure inside the placement box 6 may decrease due to the effect of the cold storage module 11, causing the protective cover 12 to be "sucked" in and difficult to open, or causing resistance when retrieving the nozzle. The presence of vents helps maintain the air pressure inside and outside each zone consistent with atmospheric pressure, ensuring smooth operation. At the same time, the vents also help to expel any trace amounts of condensation that may occur, keeping the interior dry.

[0073] Although the present invention has been described in detail above with general descriptions and specific embodiments, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

[0074] The terms such as "upper," "lower," "left," "right," and "middle" used in this specification are merely for clarity of description and are not intended to limit the scope of the invention. Any changes or adjustments to their relative relationships, without substantially altering the technical content, should also be considered within the scope of the invention.

Claims

1. A pipette tip box for immunohistochemical staining, characterized in that, include: Base (1); A fixed frame (2) is fixed to the top of the base (1); Placement box (6), which is fixed to the top of the fixing frame (2), is used to accommodate pipette tips; The movable adjustment mechanism includes a first threaded rod (3) and a movable plate (4). The first threaded rod (3) is rotatably connected to the interior of the fixed frame (2), and the movable plate (4) is threadedly connected to the first threaded rod (3). A mounting base (5) for supporting the cold storage module (11) is fixed on the movable plate (4). The partition adjustment mechanism includes a second threaded rod (8) and a first moving block (9). The second threaded rod (8) is rotatably connected to the inside of the placement box (6). The first moving block (9) is threadedly connected to the second threaded rod (8). A partition plate (10) is fixed on the first moving block (9). The movable plate (4) is configured to move the mounting base (5) out of or into the area of ​​the base (1) when the first threaded rod (3) rotates; the baffle plate (10) is configured to move within the placement box (6) when the second threaded rod (8) rotates, so as to adjust the internal space layout of the placement box (6).

2. The pipette tip box for immunohistochemical staining according to claim 1, characterized in that, The cold storage module (11) is detachably connected to the mounting base (5).

3. The pipette tip box for immunohistochemistry staining according to claim 1, wherein, A protective cover (12) is rotatably connected to the top side of the placement box (6).

4. The pipette tip box for immunohistochemical staining according to claim 1, characterized in that, The movable adjustment mechanism further includes: The first gear (13) is fixed on the first threaded rod (3); The second gear (14) is rotatably connected within the fixed frame (2) and meshes with the first gear (13); and The first rotating handle (17) has one end fixedly connected to the second gear (14) and the other end extends out of the fixed frame (2); The first threaded rod (3) is configured to be driven to rotate by the transmission of the first rotating handle (17), the second gear (14) and the first gear (13).

5. The pipette tip box for immunohistochemical staining according to claim 1, characterized in that, The baffle adjustment mechanism also includes: The third gear (15) is fixed on the second threaded rod (8); The fourth gear (16) is rotatably connected within the placement box (6) and meshes with the third gear (15); and The second rotating handle (18) has one end fixed to the fourth gear (16) and the other end extending out of the placement box (6); The second threaded rod (8) is configured to be driven to rotate by the transmission of the second rotating handle (18), the fourth gear (16) and the third gear (15).

6. The pipette tip box for immunohistochemical staining according to claim 1, characterized in that, The fixed frame (2) is also fixedly provided with a first sliding rod (19), and one end of the moving plate (4) is slidably connected to the first sliding rod (19).

7. The pipette tip box for immunohistochemical staining according to claim 1, characterized in that, The placement box (6) is also fixedly provided with a second sliding rod (20), and the partition adjustment mechanism also includes a second moving block (21). The second moving block (21) is slidably connected to the second sliding rod (20), and the second moving block (21) is fixedly connected to the partition plate (10).

8. The pipette tip box for immunohistochemical staining according to claim 3, characterized in that, The protective cover (12) is equipped with a press-type spring structure to enable quick opening and closing with one hand.

9. The pipette tip box for immunohistochemical staining according to claim 1, characterized in that, The number of the baffles (10) is multiple, and the multiple baffles (10) are arranged side by side to divide the internal space of the placement box (6) into multiple parallel independent gun head placement areas.

10. A pipette tip box for immunohistochemical staining according to claim 9, characterized in that, Each of the independent gun head placement areas has at least one vent hole at its bottom.

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