A salt-tolerant, high-efficiency polyphosphate-accumulating Vibrio alginolyticus with denitrification function and its application

Abstract

A salt-tolerant, highly efficient polyphosphate-accumulating Vibrio alginolyticus strain with denitrification capabilities and its applications have been disclosed, belonging to the field of microbial water treatment technology. The strain, named *Vibrio alginolyticus* NJ-1, was deposited at the Guangdong Provincial Microbial Culture Collection Center on April 22, 2026, with accession number GDMCC No: 68133. This strain was isolated from samples from a marine aquaculture environment and identified through isolation, purification, initial screening with BTB, secondary screening with PHB staining, and identification by 16S rRNA gene sequencing. Experiments show that this strain has a strong phosphorus removal capacity in optimized salt-containing phosphorus removal media, achieving a phosphorus removal rate of over 95% under optimal conditions; simultaneously, this strain also possesses certain ammonia nitrogen and nitrate nitrogen removal capabilities. This strain can be used for phosphorus removal treatment of saline-phosphorus wastewater and marine aquaculture tailwater. This strain is suitable for environments with salinity levels of 1%–6%.

Description

Technical Field

[0001] This invention belongs to the field of microbial water treatment technology, specifically relating to a highly efficient polyphosphate-accumulating Vibrio alginolyticus strain NJ-1 and its application in the treatment of phosphorus removal from saline and phosphorus-containing wastewater and marine aquaculture tailwater. Background Technology

[0002] High-density intensive marine aquaculture is the mainstream model of aquaculture. During the marine aquaculture process, a large amount of uneaten feed and excrement from farmed organisms lead to an increase in nitrogen and phosphorus content in the tailwater. If discharged directly without treatment, it can easily cause eutrophication of the water body and affect the ecological environment of the surrounding sea area.

[0003] Currently, nitrogen and phosphorus removal technologies for water bodies are mainly divided into three categories: physical methods, chemical methods, and biological methods. Among them, physical and chemical treatment methods have drawbacks such as high reagent costs, easy generation of secondary pollution, complex equipment operation and maintenance, and difficulty in continuous treatment, making them unsuitable for large-scale marine aquaculture wastewater treatment. Compared with physical and chemical treatment, microbial treatment has become the mainstream technology for treating aquaculture wastewater and saline wastewater due to its advantages of mild reaction conditions, lower operating costs, and suitability for continuous and large-scale treatment.

[0004] Existing research on microbial nitrogen and phosphorus removal technologies focuses on strains designed for freshwater or low-salinity environments, while resources of salt-tolerant strains are scarce. Furthermore, most salt-tolerant strains possess only single nitrogen or phosphorus removal capabilities, exhibiting low phosphorus removal efficiency and poor nitrogen and phosphorus removal capacity. This fails to meet the high-efficiency purification requirements for high-salinity marine aquaculture wastewater and phosphorus-containing industrial wastewater. Therefore, screening for a strain that maintains good phosphorus removal capacity under saline conditions while also possessing a certain nitrogen removal capacity is of practical significance for the treatment of marine aquaculture wastewater and similar saline- and phosphorus-containing wastewater. Summary of the Invention

[0005] The purpose of this invention is to address the technical problems of low phosphorus removal efficiency and narrow salinity adaptation range of existing salt-tolerant strains by providing a salt-tolerant, highly efficient polyphosphate-accumulating Vibrio alginolyticus strain that also has denitrification capabilities. This strain exhibits strong phosphorus removal ability under saline conditions and also possesses certain ammonia and nitrate nitrogen removal capabilities, making it suitable for phosphorus removal treatment of saline-phosphorus wastewater and mariculture tailwater.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] This invention provides a salt-tolerant and highly efficient polyphosphate-rich Vibrio alginolyticus bacterium with denitrification function, named Vibrio alginolyticus NJ-1, which was deposited on April 22, 2026 at the Guangdong Provincial Center for Microbial Culture Collection, with accession number GDMCC No: 68133. The address of the depository is 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou.

[0008] Preferably, the biological characteristics of Vibrio alginolyticus NJ-1 are as follows:

[0009] (1) It can grow on a solid culture medium with sodium acetate as carbon source, KNO3 as nitrogen source and K2HPO4 as phosphorus source.

[0010] (2) Round, raised, smooth, opaque, white colonies with complete and regular edges.

[0011] (3) 16S rRNA gene sequencing: 16S rRNA gene sequencing analysis was performed on Vibrio alginolyticus NJ-1. By BLAST comparison, the results showed that the 16S rRNA gene sequence of Vibrio alginolyticus NJ-1 was highly similar to that of Vibrio alginolyticus. The 16S rRNA gene sequence of this strain is shown in SEQ ID NO.1 of the sequence listing.

[0012] The present invention also provides a screening and identification method for the above-mentioned Vibrio alginolyticus NJ-1, which includes the following steps: serially diluting water samples collected from the marine aquaculture environment, plating, separating and purifying, initial screening with 1% BTB-denitrification medium, and secondary screening with PHB particle staining, thereby obtaining the sample.

[0013] This invention also provides the application of Vibrio alginolyticus NJ-1 in the phosphorus removal and purification treatment of saline and phosphorus-containing industrial wastewater and marine aquaculture tailwater. It can be used to remove ammonia nitrogen and nitrate nitrogen in saline and nitrogen-containing water bodies. One strain can treat phosphorus pollutants and nitrogen pollutants in water bodies respectively.

[0014] The specific method of the application is as follows: by inoculating Vibrio alginolyticus NJ-1, the process parameters such as carbon-nitrogen ratio, phosphorus-nitrogen ratio, salinity, pH, and temperature are precisely controlled to achieve efficient removal of phosphorus from saline water. The process is stable, has high treatment efficiency, and is suitable for large-scale engineering applications.

[0015] The cultivation conditions can be as follows: C / N ratio of 2–10, P / N ratio of 0.05–0.2, initial phosphorus concentration of 1–18 mg / L, salinity of 1%–6%, pH of 7–9, and cultivation temperature of 22–32℃; inoculum size of 3%–8% v / v, and OD of the inoculum solution of [missing information]. 600 =0.6~1.0, shaking speed 150~200r / min, processing time 36~60h.

[0016] The optimal process conditions are: C / N ratio = 5, P / N ratio = 0.05, initial phosphorus concentration = 9 mg / L, salinity = 3%, pH = 8, temperature = 27℃; inoculum size = 5% v / v, OD of inoculum solution = 600 =0.8, shaking speed 180r / min, processing time 48h.

[0017] Compared with the prior art, the present invention has at least the following beneficial effects:

[0018] 1. The Vibrio alginolyticus NJ-1 strain obtained by screening in this invention has excellent salt tolerance and is suitable for a wide range of salinity environments from 1% to 6%. It breaks through the technical bottleneck that traditional freshwater polyphosphate strains cannot be adapted to the treatment of high-salt wastewater and is suitable for the treatment of marine aquaculture tailwater and various saline and phosphorus-containing wastewater.

[0019] 2. The strain of this invention has extremely high phosphorus removal efficiency. Under optimal process conditions, the phosphorus removal rate in water can reach 98.63% in 48 hours, which is far higher than the treatment efficiency of existing conventional salt-tolerant polyphosphate strains, and the water treatment effect is excellent.

[0020] 3. The strain of this invention has nitrogen and phosphorus removal functions. The strain not only has high phosphorus removal performance, but can also degrade ammonia nitrogen and nitrate nitrogen in water, and can complete the treatment of two types of pollutants respectively; the ammonia nitrogen removal amount reaches 53.77 mg / L and the nitrate nitrogen removal amount reaches 31.38 mg / L in 48 hours, which greatly simplifies the water treatment process and reduces the treatment cost.

[0021] 4. The strain of this invention has mild culture conditions, stable characteristics, strong stress resistance, and simple and easy-to-operate treatment process. It does not require complicated equipment and expensive reagents, and is suitable for large-scale, continuous treatment of saline wastewater and aquaculture tailwater. Attached Figure Description

[0022] Figure 1 This is a graph showing the phosphorus removal effect of strain NJ-1 under different carbon source conditions in Example 2 of the present invention.

[0023] Figure 2 This is a graph showing the phosphorus removal effect of strain NJ-1 under different carbon / nitrogen ratios (C / N) in Example 3 of the present invention.

[0024] Figure 3 This is a graph showing the phosphorus removal effect of strain NJ-1 under different phosphorus / nitrogen ratios (P / N) in Example 4 of this invention.

[0025] Figure 4 This is a graph showing the phosphorus removal effect of strain NJ-1 under different phosphorus concentrations in Example 5 of the present invention.

[0026] Figure 5This is a graph showing the phosphorus removal effect of strain NJ-1 under different salinity conditions in Example 6 of the present invention.

[0027] Figure 6 This is a graph showing the phosphorus removal effect of strain NJ-1 under different pH conditions in Example 7 of this invention.

[0028] Figure 7 This is a graph showing the phosphorus removal effect of strain NJ-1 under different temperature conditions in Example 8 of the present invention. Detailed Implementation

[0029] The following embodiments, in conjunction with the accompanying drawings, will further illustrate the present invention. These embodiments are merely illustrative of the technical solutions of the present invention and should not be construed as limiting the scope of protection of the present invention. Modifications and substitutions made by those skilled in the art to the methods, steps, or conditions of the present invention without departing from the essence of the invention are all within the scope of protection of the present invention.

[0030] Unless otherwise specified, all experimental procedures in this invention employ conventional aseptic techniques and routine biochemical experimental methods. All quantitative experiments are conducted in triplicate, with the results calculated as the average of the three sets to ensure data accuracy and reliability. Unless otherwise specified, the terminology used in the following embodiments has the same meaning as commonly understood by those skilled in the art.

[0031] The culture medium involved in this invention:

[0032] (1) R2A medium (g / L): tryptone 0.25, acid hydrolyzed casein 0.50, yeast extract 0.50, soluble starch 0.50, dipotassium hydrogen phosphate 0.30, magnesium sulfate 0.10, sodium pyruvate 0.30, agar 12.00, peptone 0.25, glucose 0.50, NaCl 30.00, pH 7.20~7.40.

[0033] (2) 1% BTB denitrification medium (g / L): sodium acetate 3.01, KNO3 2.00, MgSO4·7H2O 0.20, agar 13.50, 1% BTB 4.00 mL, NaCl 30.00, pH 7.20~7.40.

[0034] (3) LB solid medium (g / L): tryptone 10.0, yeast extract 5.0, sodium chloride 30.0, pH 7.20~7.40.

[0035] (4) Optimized culture medium for biological phosphorus removal (g / L): Sodium acetate trihydrate 5.1, MgSO4·7H2O 0.15, K2HPO4 0.0506, NH4Cl 0.4584, KNO3 0.432, NaCl 30, pH 7.20~7.40. In the examples, when adjusting the components of this culture medium, unless otherwise specified, the carbon-to-nitrogen ratio (C / N) should be maintained at 5 and the phosphorus-to-nitrogen ratio (P / N) at 0.05.

[0036] (5) Nitrogen- and phosphorus-rich culture medium (g / L): Trace elements 3.09, NH4Cl 0.509, K2HPO4 0.0336, MgSO4 0.0488, KNO3 0.476, NaCl 20.00, pH 7.20~7.40.

[0037] Example 1: Isolation, screening and identification of Vibrio alginolyticus NJ-1

[0038] The water sample was taken from a seawater aquaculture pond in Nanjing Village, Zhangpu County, Zhangzhou City, Fujian Province. The sample was prepared into 10⁻¹⁰ samples using a gradient dilution method. -1 ~10 -3 Diluent was prepared and spread onto R2A agar plates. After incubation at 28°C for 48 hours, single colonies were picked and purified by streaking 3-4 times. The purified strain was inoculated into 1% BTB denitrification medium and incubated at 28°C for 48 hours. During incubation, the BTB medium changed from green to blue, indicating that the strain had potential nitrogen removal capacity. The initially screened strain was observed by PHB particle staining. The staining steps were as follows: prepare bacterial smears and heat fix; stain with 0.3% Sudan Black for 10 min; wash with 70% ethanol to remove excess dye; wash with water and blot dry; counterstain with safranin for 1 min; wash with water and blot dry; observe under a 1000x oil immersion microscope. The microscopic results showed the presence of blue-black PHB particles in the bacterial cells, indicating that the strain had polyphosphate-accumulating bacteria screening characteristics. The target strain was numbered NJ-1.

[0039] Morphological identification: After being cultured on R2A and LB media, Vibrio alginolyticus NJ-1 colonies were round, raised, smooth, opaque, with complete and regular edges, and were white in color. The colony characteristics were stable.

[0040] Molecular biological identification: The screened and purified strain was sent to Zhejiang Youkang Biotechnology Co., Ltd. for 16S rRNA sequencing. The sequencing results showed that the 16S rRNA gene sequence of strain NJ-1 is shown in SEQ ID NO.1 of the sequence listing. BLAST alignment analysis of the sequencing results showed that the sequence had 99.79% similarity to the 16S rRNA sequence of Vibrio alginolyticus 5-33 (MW080015.1). Therefore, the screened strain was identified as Vibrio alginolyticus and named Vibrio alginolyticus NJ-1, abbreviated as strain NJ-1.

[0041] Example 2: Effect of carbon source type on phosphorus removal efficiency of strain NJ-1 in water

[0042] The carbon source types in the biological phosphorus removal optimization medium were adjusted, with four carbon source groups set up: sodium acetate, sodium citrate, sodium succinate, and glucose. The amount of carbon source added was adjusted to ensure the same carbon content in each group, while maintaining other medium components and parameters consistent. The initial OD was increased to 5% v / v. 600 NJ-1 seed culture at a concentration of 0.8 was inoculated into biological phosphorus removal optimization culture media containing different carbon sources. After culturing at 27℃ and 180 r / min for 48 h, water samples were taken to calculate the phosphorus removal rate of each group of water bodies.

[0043] Experimental results showed that different carbon sources significantly affected the phosphorus removal capacity of strain NJ-1. When sodium acetate was used as the sole carbon source, the strain exhibited the strongest proliferation activity and the highest phosphorus accumulation efficiency. The effects of sodium acetate and sodium succinate treatments were similar, with both showing better phosphorus removal efficiency than glucose and sodium citrate groups. Therefore, sodium acetate was determined to be the optimal carbon source for strain NJ-1 (see results below). Figure 1 ).

[0044] Example 3: Effect of C / N ratio on phosphorus removal efficiency of strain NJ-1 in water

[0045] Using sodium acetate as the carbon source, the C / N ratio of the biological phosphorus removal medium was adjusted by varying the amount of sodium acetate added. Six gradient groups (0, 2, 5, 10, 15, and 20) were set up, with other conditions remaining constant. The initial OD was inoculated at a rate of 5% v / v. 600 0.8 NJ-1 seed culture was inoculated into biological phosphorus removal optimization medium with different carbon-nitrogen ratios (C / N). After culturing at 27℃ and 180 r / min for 48 h, water samples were taken to calculate the phosphorus removal rate of each group.

[0046] Experimental results are as follows Figure 2As shown, strain NJ-1 achieved the highest phosphorus removal rate in the water sample when the carbon-to-nitrogen ratio (C / N) was 5, which is the optimal C / N ratio parameter. Too low a C / N ratio would restrict the growth and metabolism of the strain and result in insufficient phosphorus accumulation capacity. Too high a C / N ratio would cause an imbalance in the carbon-to-nitrogen ratio, which would not be conducive to the strain maintaining efficient phosphorus accumulation activity and would lead to a decrease in phosphorus removal efficiency.

[0047] Example 4: Effect of phosphorus / nitrogen ratio (P / N) on phosphorus removal efficiency of strain NJ-1 in water

[0048] The phosphorus-to-nitrogen ratio (P / N) of the biological phosphorus removal medium was adjusted by varying the amounts of NH4Cl and KNO3 added, with five gradient groups of 0.05, 0.1, 0.2, 0.4, and 0.8. Simultaneously, the amount of sodium acetate added was adjusted to ensure a C / N ratio of 5 for each group, while keeping other conditions constant. The initial OD was then inoculated at a rate of 5% v / v. 600 Seed culture of 0.8 was inoculated into biological phosphorus removal optimization medium with different phosphorus / nitrogen ratios (P / N). After culturing at 27℃ and 180 r / min for 48 h, water samples were taken to calculate the phosphorus removal rate.

[0049] Experimental results show that an excessively high P / N ratio exceeds the phosphorus accumulation metabolic load of the strain, leading to a significant decrease in phosphorus removal efficiency. *Vibrio alginolyticus* NJ-1 exhibits the highest phosphorus removal rate in the water sample at a P / N ratio of 0.05, representing the optimal phosphorus-nitrogen ratio parameter. (See results below.) Figure 3 As shown.

[0050] Example 5: Effect of phosphorus concentration on phosphorus removal efficiency of strain NJ-1 in water

[0051] The phosphorus concentration in the biological phosphorus removal optimization medium was adjusted by varying the amount of K₂HPO₄ added to 1, 5, 9, 18, and 27 mg / L. Simultaneously, the amounts of other components were adjusted to ensure optimal C / N and P / N ratios, while other conditions remained constant. The initial OD was then inoculated at a 5% v / v inoculum. 600 Seed culture at a concentration of 0.8 was inoculated into biological phosphorus removal optimization culture media with different phosphorus concentrations. After culturing at 27℃ and 180 r / min for 48 h, water samples were taken to calculate the phosphorus removal rate.

[0052] The results showed that at low phosphorus concentrations, the strain had insufficient nutrient substrates, resulting in low phosphorus removal efficiency; high phosphorus concentrations inhibited the strain's metabolism; strain NJ-1 exhibited the best phosphorus removal performance at an initial phosphorus concentration of 9 mg / L, making it suitable for treating saline and phosphorus-containing water bodies with this concentration gradient, as shown in the results. Figure 4 As shown.

[0053] Example 6: Effect of salinity on phosphorus removal efficiency of strain NJ-1 in water

[0054] The salinity of the biological phosphorus removal optimization medium was adjusted to eight gradients: 0%, 1%, 2%, 3%, 4%, 5%, 6%, and 8%. The initial OD was increased to 5% v / v. 600 Seed culture at a concentration of 0.8 was inoculated into optimized biological phosphorus removal media with different salinities. After culturing at 27℃ and 180 r / min for 48 h, water samples were taken to calculate the phosphorus removal rate.

[0055] Experimental results showed that strain NJ-1 possesses excellent salt tolerance, stably performing phosphorus removal within a salinity range of 1%–6%. At a salinity of 3%, the strain exhibited the strongest proliferation and polyphosphate accumulation activity, resulting in the highest phosphorus removal rate in the water sample. However, when the salinity exceeded 6%, the strain's metabolic activity was inhibited, and the phosphorus removal efficiency significantly decreased. The results are as follows: Figure 5 As shown.

[0056] Example 7: Investigation on the effect of pH on phosphorus removal efficiency of strain NJ-1 in water

[0057] The pH of the biological phosphorus removal medium was adjusted to optimize five acid-base gradients: 5, 6, 7, 8, and 9. The initial OD was then inoculated at a rate of 5% v / v. 600 Seed culture at 0.8 was inoculated into biological phosphorus removal optimization culture media with different pH values. After culturing at 27℃ and 180 r / min for 48 h, water samples were taken to calculate the phosphorus removal rate of each group.

[0058] Experimental results showed that strain NJ-1 is adapted to a weakly alkaline water environment, while acidic and strongly alkaline environments inhibit its enzyme activity and metabolic function. At pH 8, the strain exhibited the optimal polyphosphate metabolism efficiency and the highest phosphorus removal rate in the water sample. (See results below.) Figure 6 As shown.

[0059] Example 8: Effect of temperature on phosphorus removal efficiency of strain NJ-1 in water

[0060] The shaker temperatures were adjusted to 22℃, 27℃, 32℃, 37℃, and 42℃, and the initial OD was inoculated at a rate of 5% v / v. 600 The seed culture solution of 0.8 was inoculated into the optimized biological phosphorus removal medium. After culturing for 48 h at different temperatures and 180 r / min, water samples were taken to calculate the phosphorus removal rate of each group.

[0061] The results showed that low temperatures slowed the growth and metabolism of the strain, while high temperatures led to protein denaturation and enzyme inactivation. 27℃ was the optimal culture temperature for strain NJ-1, at which temperature the strain achieved the highest phosphorus removal rate. (See results below.) Figure 7 As shown.

[0062] Verification of optimal process parameters: According to Examples 2 to 7, it can be concluded that strain NJ-1 can achieve a maximum phosphorus removal rate of 98.63% under the following conditions: sodium acetate as carbon source, C / N ratio of 5, P / N ratio of 0.05, phosphorus concentration of 9 mg / L, salinity of 3%, pH of 8, and temperature of 27°C.

[0063] Example 9: Detection of nitrogen removal function of strain NJ-1 in water

[0064] Strain NJ-1 was inoculated into LB liquid medium and cultured at 180 rpm and 27°C for 2 days. After culture, the bacterial cells were collected by centrifugation and resuspended in nitrogen- and phosphorus-rich medium, and cultured at 180 rpm and 27°C for 2 days. After culture, the supernatant was collected by centrifugation, and the changes in ammonia nitrogen and nitrate nitrogen content were measured.

[0065] The test results showed that after 48 hours of cultivation, strain NJ-1 removed 53.77 mg / L of ammonia nitrogen and 31.38 mg / L of nitrate nitrogen from the water. This indicates that strain NJ-1, in addition to its polyphosphate accumulation ability, also possesses the ability to degrade both ammonia nitrogen and nitrate nitrogen, thus enabling the treatment of nitrogen and phosphorus in saline water bodies.

[0066] This invention provides a salt-tolerant, highly efficient polyphosphate-accumulating Vibrio alginolyticus strain with denitrification capabilities, named Vibrio alginolyticus NJ-1. This strain possesses both polyphosphate accumulation metabolism and denitrification metabolism, enabling it to degrade phosphorus and nitrogen pollutants in water bodies, respectively. Since the polyphosphate accumulation pathway and the denitrification pathway compete for carbon sources, this invention ensures the stable expression of both physiological functions by precisely controlling the C / N and P / N ratios. This invention is the first to develop Vibrio alginolyticus into a salt-tolerant polyphosphate-accumulating strain, creating a new application for the strain. Most existing salt-tolerant strains only possess single phosphorus removal or single denitrification capabilities; this strain can tolerate salinity of 1%–6%, achieving a phosphorus removal rate of up to 98.63% under optimal conditions, while simultaneously degrading ammonia nitrogen and nitrate nitrogen, thus combining two types of water treatment functions in a single strain. Conventional freshwater polyphosphate-accumulating bacteria rapidly lose their polyphosphate activity when salinity exceeds 1%. This strain overcomes the inhibition of polyphosphate accumulation by high salt osmotic pressure, solving the industry problem of low biological phosphorus removal efficiency under high salinity conditions in marine aquaculture tailwater. Through multiple sets of single-factor gradient experiments, the complete set of process parameters, including carbon source, carbon-nitrogen ratio, phosphorus-nitrogen ratio, salinity, pH, temperature, and inoculum size, were systematically optimized to form a replicable saline wastewater treatment process.

[0067] The above embodiments are merely preferred embodiments of the present invention and do not constitute a limitation on the technical solution of the present invention. Conventional modifications made by those skilled in the art to the strain culture conditions, inoculation processes, and wastewater treatment parameters without departing from the concept of the present invention should be included within the protection scope of the present invention.

Claims

1. A salt-tolerant, highly efficient polyphosphate-accumulating Vibrio alginolyticus with denitrification function, characterized in that... The strain is Vibrio alginolyticus NJ-1, which was deposited at the Guangdong Provincial Center for Microbial Culture Collection on April 22, 2026, with accession number GDMCC No: 68133.

2. The salt-tolerant, high-efficiency polyphosphate-rich alginolytic Vibrio bacterium with denitrification function according to claim 1, characterized in that, The 16S rRNA gene sequence of Vibrio alginolyticus NJ-1 is shown in SEQ ID NO.1 of the sequence listing; the strain was grown on a solid medium with sodium acetate as the carbon source, KNO3 as the nitrogen source and K2HPO4 as the phosphorus source.

3. The application of Vibrio alginolyticus NJ-1 according to claim 1 in phosphorus removal from saline and phosphorus-containing wastewater or marine aquaculture tailwater.

4. The application of Vibrio alginolyticus NJ-1 according to claim 1 in reducing ammonia nitrogen and / or nitrate nitrogen in saline bodies.

5. The application according to claim 3, characterized in that, The saline and phosphorus-containing wastewater includes marine aquaculture tailwater and saline industrial phosphorus-containing wastewater.

6. The application according to claim 3 or 4, characterized in that, It is suitable for environments with a salinity of 1% to 6%.

7. A method for removing phosphorus from saline and phosphorus-containing wastewater, characterized in that, The Vibrio alginolyticus NJ-1 described in claim 1 was inoculated into the saline and phosphorus-containing water body to be treated for cultivation treatment to reduce the total phosphorus content of the water body.

8. The method according to claim 7, characterized in that, The cultivation conditions were as follows: C / N ratio of 2–10, P / N ratio of 0.05–0.2, initial phosphorus concentration of 1–18 mg / L, salinity of 1%–6%, pH of 7–9, and cultivation temperature of 22–32℃; inoculum size of 3%–8% v / v, and OD of the inoculum solution of [missing information]. 600 =0.6~1.0, shaking speed 150~200r / min, processing time 36~60h.

9. The method according to claim 8, characterized in that, The optimal conditions for cultivation were: C / N ratio of 5, P / N ratio of 0.05, initial phosphorus concentration of 9 mg / L, salinity of 3%, pH of 8, and temperature of 27℃; inoculum size of 5% v / v; and OD of the inoculum solution. 600 =0.8, shaking speed 180r / min, processing time 48h.

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