A method for the propagation and application of scandium extract for inactivated probiotics

Abstract

This invention relates to the field of rare metal metallurgy, and provides a method for the preparation and application of an inactivated probiotic scandium metal extractant, solving the problems of unsafety and environmental unfriendliness of existing methods for extracting scandium using sulfuric acid, hydrochloric acid, nitric acid, and high-temperature roasting. The method includes the following processing steps: (1) preparing a 2.0×3... 7 (1) Take one of the following probiotic dormant bacteria, Lactobacillus spores or Bacillus licheniformis, at a concentration of 2.3-4.1 g / L sugar, 5.3-7.1 g / L rice bran, and 3.3-7.1 g / L flour as feed; awaken and incubate the bacteria in a constant temperature environment at pH 7-7.2 and 30-32℃ for 70-72 hours; (2) inactivate the probiotics; (3) separate the solution from the feed using a centrifuge at a speed of not less than 4000 rpm for 5 minutes, and take the remains solution as the scandium extractant for inactivated probiotics.

Description

Technical Field

[0001] This invention relates to the field of rare metal metallurgy, and to a method for extracting scandium from strongly alkaline red mud or other minerals with a pH of 12.1-13.0 using a green probiotic scandium extraction agent technology. In particular, it relates to a method for breeding and applying an inactivated probiotic anti-extraction agent. Background Technology

[0002] Scandium is a rare and dispersed metal on Earth. Due to its unique physical and chemical properties, it holds significant strategic importance and value in aerospace, medical applications, laser crystals, fuel cells, and especially in the development of advanced aluminum-scandium alloys for military use. Traditional scandium extraction methods require high-acid, high-temperature processes. For example, Chinese Patent Publication No. CN1844421, published on October 11, 2006, discloses a method for extracting scandium from red mud, which includes: adding 300L of 24% hydrochloric acid to an acid-resistant reaction vessel, then adding 50kg of red mud, heating to 70℃, reacting for 1 hour, and filtering to obtain 260L of a scandium-containing leachate with a scandium content of 14.3mg / L, representing a leaching rate of 90.7%. For example, Chinese Patent Publication No. CN102653820A discloses a method for extracting scandium from Bayan Obo tailings, comprising: S1, mixing the tailings with one or more activators selected from calcium chloride, barium chloride, magnesium chloride, and strontium chloride in a weight ratio of 1:0.3 to 1.3; S2, roasting the mixture for 0.5 to 4 hours at a roasting temperature of 700℃ to 1200℃; S3, washing the roasted ore obtained by roasting with hot water to form roasted ore and washing solution; S4, dissolving and leaching the washed roasted ore with an inorganic acid of sulfuric acid, hydrochloric acid, or nitric acid, with a leaching rate of over 85%. The problems are as follows: First, patent CN1844421 uses 24% hydrochloric acid (300L) in a "chlorine leaching" method with 50kg of red mud heated to 70℃ to extract metals. The contact between this highly acidic hydrochloric acid (pH 0-1) and the highly alkaline red mud (pH 12.1-13.0) is extremely likely to cause an explosion, even at room temperature (35℃), posing a safety risk in large-scale production. Second, patent CN102653820A reuses sulfuric acid, hydrochloric acid, nitric acid, and barium chloride as leaching agents or activators, causing serious environmental pollution. Summary of the Invention

[0003] Therefore, in view of the above problems, the present invention provides a method for the breeding, preparation and application of scandium metal extractant from inactivated probiotics, which solves the problems of unsafety and environmental unfriendliness of existing methods for extracting scandium using sulfuric acid, hydrochloric acid, nitric acid and high-temperature roasting.

[0004] To achieve the above objectives, the present invention adopts the following technical solution: a method for propagating inactivated probiotic scandium extract, comprising the following processing steps:

[0005] (1) The concentration is 2.0×37 Probiotic dormant cells of either Bacillus spores or Bacillus licheniformis were prepared using one part each of sugar (2.3-4.1 g / L), rice bran (5.3-7.1 g / L), and flour (3.3-7.1 g / L) as feed; and were awakened and propagated for 70-72 hours in a thermostat with pH 7-7.2 and shaking at 30-32℃.

[0006] (2) Inactivate probiotics;

[0007] (3) Use a centrifuge at a speed of not less than 4000 rpm for 5 minutes to separate the solution from the animal remains, and take the solution of the remains as the inactivated probiotic scandium extract.

[0008] Furthermore, in step (1), after 70-72 hours, a concentration of 2.0 × 20⁻⁶ was produced. 7 Probiotics per mL.

[0009] Furthermore, after 70-72 hours of incubation in step (1), the probiotic cell wall is 20-80 nm thick, the thickness of the negatively charged "δ-" peptidoglycan synthesized on and outside the cell membrane is 45-70 nm / cell, the thickness of the negatively charged "δ-" teichoic acid is 5-8 nm / cell, the potential is φ=60mV / mL, and the electronegativity χ=2.2.

[0010] Furthermore, the probiotic scandium extract in step (1) is characterized by the fact that the negative charge-dependent probiotic C-type cytochromes enable the transfer of electron energy between intracellular and extracellular substances.

[0011] Furthermore, the method for inactivating probiotics in step (2) is to boil the probiotics for 5-8 minutes to inactivate them.

[0012] Furthermore, after the solution in step (3) is separated from the feed, the feed can be reused several times.

[0013] A method for applying an inactivated probiotic scandium extract includes the following processing steps:

[0014] (1) Add the above-mentioned inactivated probiotic scandium extract to red mud or other scandium-containing minerals with a pH of 12.1-13.0 (no pH or temperature treatment is required);

[0015] (2) Stir at room temperature for 1-1.5 hours at a speed of not less than 150 rpm using a stirrer; separate using a centrifuge at a speed of not less than 4000 rpm for 5-10 minutes to obtain the leached scandium metal.

[0016] Furthermore: When the inactivated probiotic scandium extract described in step (1) is added to red mud or other scandium-containing minerals, the outermost layer of scandium metal is positively charged Sc. 3+The electronegativity is χ = 1.63; in step (2), when the inactivated probiotic scandium extract is added to the red mud and stirred, the negative charge of the inactivated probiotic scandium extract "δ - "and scandium metal δ in red mud" + "charge Sc3 + Energy entanglement occurs, forming an attraction complexation reaction; after stirring for 1-1.5 hours in step (2), stable ionic bonds are formed, and after solid-liquid separation, the scandium recovery rate is over 99.1%.

[0017] By adopting the aforementioned technical solution, the beneficial effects of the present invention are as follows:

[0018] This invention is based on the principle of "opposite charges attract": *Lactobacillus sporeans* or *Bacillus licheniformis* are probiotics with intracellular electron energy transfer and extracellular electron transport mechanisms. These probiotics generate a negative charge "δ" on their cell walls to maintain the stability of the intracellular and extracellular environment and regulate cell growth. - The peptidoglycan and teichoic acid in cytochrome C facilitate electron energy transfer between intracellular and extracellular substances. After inactivation, they carry a negative charge "δ". - "Without changing potential and electronegativity, it reacts with positively charged SC after being added to red mud." 3+ Scandium metal with electronegativity χ = 1.63 forms a tightly entangled complex reaction. After stirring for 1-1.5 hours, the solid and liquid separate, and the metal becomes stable by forming ionic bonds. This is exactly the law of "opposite charges attract each other".

[0019] The advantages of this invention are as follows: First, it achieves a scandium recovery rate of over 99.1% based on the principle of "opposite charges attract". Second, *Lactobacillus spores* or *Bacillus licheniformis* are among the earliest discovered probiotic species, fermenting bacteria that can be used as food products and do not produce substances harmful to the human body. The inactivated scandium extract from these beneficial bacteria exhibits significant environmental benefits in an ecologically green and zero-pollution cycle. Third, *Lactobacillus spores* or *Bacillus licheniformis* are inexpensive and easily propagated bacteria, resulting in low costs for propagating, inactivating, preparing, and applying the scandium extract. Detailed Implementation

[0020] Example 1

[0021] A method for propagating scandium metal extracts from inactivated probiotics includes the following processing steps:

[0022] 1) The concentration is 2.0×3 7 Probiotic dormant cells of *Lactobacillus spores* (at a concentration of 2.0 × 10⁶ mL) were incubated for 70 hours on a shaker at a pH of 7.2 and a constant temperature of 30°C, using equal parts of 2.3 g / L sugar, 5.3 g / L rice bran, and 3.3 g / L flour as feed; the resulting product had a concentration of 2.0 × 10⁶ mL. 7 Probiotics of 1 / mL were inactivated by boiling for 5 minutes.

[0023] 2) The solution was separated from the animal remains using a centrifuge at 4500 rpm for 5 minutes. The solution from the remains was used as an extractant for inactivated probiotics.

[0024] 3) After separating the solution from the feed, the feed can be reused several times.

[0025] The application of an inactivated probiotic scandium extract involves the following processing steps:

[0026] 1) Add the above-bred inactivated probiotic scandium extract to red mud (pH 12.1) without any treatment of pH or temperature;

[0027] 2) Stir at a speed of not less than 150 rpm for 1 hour using a laboratory stirrer at room temperature; then separate in a centrifuge at 4500 rpm for 5 minutes, with a scandium leaching rate of 99.1%.

[0028] Example 2

[0029] A method for preparing a probiotic scandium extract, comprising the following steps:

[0030] 1) The concentration is 2.0×3 8 Probiotic dormant cells of Bacillus licheniformis were propagated using equal parts of 3 g / L sugar, 6 g / L rice bran, and 6 g / L flour in a shaker at pH 7.1 and a constant temperature of 31°C for 71 hours, yielding a concentration of 2.0 × 20⁻⁶. 8 Probiotics were inactivated by boiling at 5 nm for 1 / ML.

[0031] 2) The solution and the animal were separated by centrifugation at 4300 rpm for 5 min. The solution from the remains was used as the scandium extract for inactivated probiotics.

[0032] 3) After separating the solution from the feed, the feed can be reused several times.

[0033] The application of an inactivated probiotic scandium extract involves the following processing steps:

[0034] 1) Add the above-bred inactivated probiotic scandium extract to scandium-containing minerals with a pH of 12.5 (no pH or temperature treatment is required);

[0035] 2) Stir at a speed of not less than 150 rpm for 1.3 hours using a laboratory stirrer at room temperature; then separate in a centrifuge at 4300 rpm for 5 minutes, with a scandium leaching rate of 99.2%.

[0036] Example 3

[0037] A method for preparing a probiotic scandium extract, comprising the following steps:

[0038] The concentration is 2.0×3 9 A concentration of *Lactobacillus spores* probiotic dormant bacteria ( / mL) was added to 4.2 g / L sugar, 7.3 g / L rice bran, and 7.3 g / L flour at a solid-liquid ratio of 1:15. The mixture was then awakened and incubated for 72 hours at 32℃ using a laboratory thermostat with shaking, yielding a concentration of 2.0 × 20⁻⁶. 9 The bacteria were inactivated at a concentration of 100 cells / mL and then subjected to solid-liquid separation in a laboratory centrifuge at 4800 rpm for 8 minutes. The solution was then separated from the animal remains, and the remains solution was used as the scandium extract for inactivated probiotics. After separation of the solution from the animal remains, the animal remains could be reused several times.

[0039] A method for applying a probiotic scandium extract includes the following processing steps:

[0040] (1) The above-prepared probiotic scandium extract is added to red mud with a pH of 13.0. No treatment is required for pH or temperature.

[0041] (2) Stir at a speed of not less than 150 rpm for 1.5 h using a laboratory stirrer at room temperature; leaching is performed in a centrifuge at a speed of 4800 rpm for 8 min, with scandium leaching reaching 99.3%.

[0042] Although the invention has been specifically shown and described in conjunction with preferred embodiments, those skilled in the art should understand that various changes in form and detail may be made to the invention without departing from the spirit and scope of the invention as defined in the appended claims, all of which shall be within the scope of protection of the invention.

Claims

1. A method for propagating inactivated probiotic scandium extract, characterized in that, The following processing steps are included: (1) The concentration is 2.0×3 7 Probiotic dormant cells of either Bacillus spores or Bacillus licheniformis were prepared using one part each of sugar (2.3-4.1 g / L), rice bran (5.3-7.1 g / L), and flour (3.3-7.1 g / L) as feed; and were awakened and propagated for 70-72 hours in a thermostat with pH 7-7.2 and shaking at 30-32℃. (2) Inactivate probiotics; (3) Use a centrifuge at a speed of not less than 4000 rpm for 5 minutes to separate the solution from the animal remains, and take the solution of the remains as the inactivated probiotic scandium extract.

2. The method for propagating inactivated probiotics using scandium metal extract according to claim 1, characterized in that, Step (1), after 70-72 hours, a concentration of 2.0×20 was obtained. 7 Probiotics per mL.

3. The method for propagating inactivated probiotics using scandium metal extract according to claim 1, characterized in that, After 70-72 hours of incubation in step (1), the probiotic cell wall is 20-80 nm thick, the thickness of the negatively charged "δ-" peptidoglycan synthesized on and outside the cell membrane is 45-70 nm / cell, the thickness of the negatively charged "δ-" teichoic acid is 5-8 nm / cell, the potential is φ=60mV / mL, and the electronegativity χ=2.

2.

4. The method for propagating inactivated probiotics using scandium metal extract according to claim 3, characterized in that, Step (1) The probiotic scandium extract is characterized by the fact that the negative charge-dependent probiotic C-type cytochromes realize the transfer of electron energy between intracellular and extracellular substances.

5. The method for propagating inactivated probiotics using scandium metal extract according to claim 1, characterized in that, Step (2) The method for inactivating probiotics is to boil the probiotics for 5-8 minutes to inactivate them.

6. A method for propagating inactivated probiotics using scandium metal extract as described in claim 1, characterized in that, After the solution in step (3) is separated from the feed, the feed can be reused several times.

7. A method for applying an inactivated probiotic scandium extract, characterized in that, The following processing steps are included: (1) The inactivated probiotic scandium extract of any one of claims 1 to 6 is added to red mud or other scandium-containing minerals with a pH of 12.1-13.0; (2) Stir at room temperature for 1-1.5 hours at a speed of not less than 150 rpm using a stirrer; separate using a centrifuge at a speed of not less than 4000 rpm for 5-10 minutes to obtain the leached scandium metal.

8. The method of applying the scandium extract of inactivated probiotics according to claim 7, characterized in that: When the scandium extract of inactivated probiotics described in step (1) is added to red mud or other scandium-containing minerals, the outermost layer of scandium metal is positively charged Sc. 3+ The electronegativity is χ=1.63; in step (2), when the inactivated probiotic scandium extract is added to the red mud and stirred, the negative charge of the inactivated probiotic scandium extract "δ - "Scandium metal δ in red mud" + "charge Sc3 + Energy entanglement occurs, forming an attraction complexation reaction; after stirring for 1-1.5 hours in step (2), stable ionic bonds are formed, and after solid-liquid separation, the scandium recovery rate is over 99.1%.