A soothing antibacterial anti-hair loss hair care formulation of a polypeptide in synergy with a plant active and a method of making the same

Abstract

This invention belongs to the field of scalp care formulation technology, and discloses a soothing, antibacterial, anti-hair loss, and hair-strengthening formulation with peptides and plant active ingredients, and its preparation method. The formulation includes a stabilized peptide-plant active ingredient pre-complex liquid, a matrix moisturizer, a scalp conditioner, a thickener, a pH adjuster, a preservative, and deionized water. The pre-complex liquid is prepared by pre-dispersion, weakly acidic pre-complexation, and stabilization treatment of licorice extract, scutellaria baicalensis extract, rosemary extract, hydrolyzed keratin peptides, plant-derived hydrolyzed peptides, glycerin, 1,3-propanediol, β-glucan, and sodium hyaluronate. In preparation, a plant active ingredient pre-dispersion liquid is first formed, then a low-molecular-weight peptide complex is added, followed by β-glucan and sodium hyaluronate for stabilization, and finally mixed with the formulation matrix and the pH is adjusted. This invention is beneficial for improving the dispersion stability, scalp and hair surface retention, and antibacterial persistence of plant active ingredients, and is suitable for soothing, antibacterial, anti-hair loss, and hair-strengthening care.

Description

Technical Field

[0001] This invention belongs to the field of scalp care preparation technology, specifically relating to a soothing, antibacterial, anti-hair loss and hair-strengthening preparation containing polypeptides and plant active ingredients, and its preparation method. Background Technology

[0002] With the increasing demand for scalp care, soothing, antibacterial, and hair-strengthening products targeting scalp sensitivity, sebum imbalance, scalp microecological disorders, increased dandruff, and increased hair loss during combing are gaining attention. Currently, research in this field mainly focuses on the formulation of plant-based active ingredients, the application of peptide-based hair conditioning ingredients, and the construction of gentle scalp care matrices. For example, plant-derived active ingredients such as licorice extract, scutellaria baicalensis extract, and rosemary extract, containing components such as glycyrrhizic acid, baicalin, and rosmarinic acid, are widely used in soothing, antibacterial, and antioxidant scalp care products. Low-molecular-weight peptides such as hydrolyzed keratin peptides and plant-derived hydrolyzed peptides are often used to improve hair surface conditioning, enhance the gentleness of scalp care formulations, and improve the user experience. Meanwhile, polysaccharide raw materials such as β-glucan and sodium hyaluronate are also frequently used as moisturizing, soothing, or system-stabilizing auxiliary ingredients in daily chemical formulations.

[0003] However, existing soothing, antibacterial, and hair loss-preventing preparations still have certain shortcomings. On the one hand, some products mainly rely on the simple superposition of multiple plant extracts or functional raw materials, lacking reasonable compatibility control between active ingredients, which easily leads to batch-to-batch fluctuations in efficacy. On the other hand, the active ingredients in plant extracts are complex in composition and are easily affected by pH, temperature, solvent environment, and storage conditions in aqueous systems, resulting in phenomena such as color deepening, precipitation, or decreased stability. In addition, although peptide raw materials have good hair and scalp conditioning value, their dispersion state in complex systems, compatibility with other active ingredients, and surface retention ability after application still need improvement. Existing products also find it difficult to simultaneously achieve gentleness, antibacterial long-lasting effect, active ingredient stability, and hair loss-preventing and hair-strengthening effects. Summary of the Invention

[0004] To address the shortcomings mentioned in the background art, the present invention aims to provide a soothing, antibacterial, anti-hair loss and hair-strengthening preparation of a polypeptide-plant active ingredient synergistic formula and its preparation method. The preparation comprises a stabilized polypeptide-plant active ingredient pre-complex liquid, a matrix moisturizer, a scalp conditioner, a thickener, a pH adjuster, a preservative, and deionized water. The pre-complex liquid is prepared by pre-dispersion, pre-complexation, and stabilization treatment of licorice extract, scutellaria baicalensis extract, rosemary extract, a low molecular weight polypeptide combination, glycerin, 1,3-propanediol, β-glucan, and sodium hyaluronate.

[0005] The objective of this invention can be achieved through the following technical solutions: A soothing, antibacterial, anti-hair loss and hair-strengthening preparation containing polypeptides and plant active ingredients, comprising, by weight, 15-35 parts of a stabilized polypeptide-plant active ingredient pre-complex solution, 3-10 parts of a matrix moisturizer, 0.5-3 parts of a scalp conditioner, 0.1-0.6 parts of a thickener, 0.05-0.5 parts of a pH adjuster, 0.3-1.0 parts of a preservative, and deionized water to a total of 100 parts; The stabilized polypeptide-plant active ingredient pre-composite solution is prepared by pre-dispersion, pre-composite, and stabilization treatment of standardized plant active ingredients, low molecular weight polypeptide combination, polyol aqueous phase system, and stabilized polysaccharide; the low molecular weight polypeptide combination consists of hydrolyzed keratin peptides and plant-derived hydrolyzed polypeptides, and the molecular weight of both hydrolyzed keratin peptides and plant-derived hydrolyzed polypeptides is 300-3000 Da, and the peptide content is not less than 80%; the standardized plant active ingredients are licorice extract, scutellaria baicalensis extract, and rosemary extract; the low molecular weight polypeptide combination consists of hydrolyzed keratin peptides and plant-derived hydrolyzed polypeptides; the polyol aqueous phase system consists of glycerol, 1,3-propanediol, and deionized water; the stabilized polysaccharide includes β-glucan and sodium hyaluronate.

[0006] More preferably, based on 100 parts of the stabilized polypeptide-plant active ingredient pre-complex solution, the stabilized polypeptide-plant active ingredient pre-complex solution comprises 1.0-3.5 parts of licorice extract, 1.0-4.0 parts of scutellaria baicalensis extract, 0.5-2.5 parts of rosemary extract, 1.5-4.5 parts of hydrolyzed keratin peptide, 1.0-3.5 parts of plant-derived hydrolyzed polypeptide, 0.2-2.0 parts of β-glucan, 0.02-0.5 parts of sodium hyaluronate, 10-25 parts of glycerin, 6-18 parts of 1,3-propanediol, and deionized water to make up to 100 parts.

[0007] More preferably, based on the mass of the corresponding extracts, the licorice extract contains 5% to 20% glycyrrhizic acid, the scutellaria extract contains 10% to 40% baicalin, and the rosemary extract contains 2% to 10% rosmarinic acid; in the stabilized polypeptide-plant active ingredient precomplex solution, the mass ratio of glycyrrhizic acid, baicalin, and rosmarinic acid is 1:(0.8 to 2.5):(0.2 to 1.0).

[0008] More preferably, in the low molecular weight polypeptide combination, the mass ratio of the hydrolyzed keratin peptide to the plant-derived hydrolyzed polypeptide is 1:(0.5-2.0) based on dry weight.

[0009] More preferably, the plant-derived hydrolyzed polypeptide is one or more of hydrolyzed soybean protein peptide, hydrolyzed pea protein peptide, hydrolyzed oat protein peptide, and hydrolyzed wheat protein peptide.

[0010] More preferably, the mass ratio of β-glucan to sodium hyaluronate is 1:(0.05~0.5).

[0011] More preferably, the base moisturizer is one or more of glycerin, 1,3-propanediol, and betaine; the scalp conditioning agent is one or more of panthenol, nicotinamide, allantoin, and sodium pyrrolidone carboxylate; the thickener is one or more of hydroxyethyl cellulose, xanthan gum, and carbomer; the pH adjuster is one or more of citric acid, lactic acid, arginine, and sodium hydroxide; and the preservative is one or more of phenoxyethanol, ethylhexylglycerin, p-hydroxyacetophenone, and capryloyl hydroxamic acid.

[0012] A method for preparing a soothing, antibacterial, anti-hair loss, and hair-strengthening preparation containing polypeptides and plant active ingredients includes the following steps: S1. Glycyrrhiza extract, Scutellaria baicalensis extract and rosemary extract are added to glycerol, 1,3-propanediol and the first part of deionized water, and stirred to disperse to obtain a standardized pre-dispersion of plant active ingredients; S2. Add the first part of the pH adjuster to the standardized plant active substance pre-dispersion solution to adjust the pH of the system, then add hydrolyzed keratin peptide and plant-derived hydrolyzed polypeptide, stir and combine to obtain polypeptide-plant active substance pre-composite solution; S3. After pre-wetting β-glucan and sodium hyaluronate with the second part of deionized water, add them to the polypeptide-plant active ingredient precomplex solution, stir to stabilize, and obtain a stabilized polypeptide-plant active ingredient precomplex solution. S4. Add the thickener to the deionized water in the third part, disperse and hydrate it, then add the matrix moisturizer, scalp conditioner and preservative, stir evenly to obtain the formulation matrix; S5. Add the stabilized polypeptide-plant active ingredient pre-complex solution obtained in step S3 to the formulation matrix, stir evenly, and add the second part of pH adjuster to adjust the pH of the system to obtain the soothing, antibacterial, anti-hair loss and hair strengthening formulation of polypeptide synergistic with plant active ingredients.

[0013] More preferably, in step S2, before adding hydrolyzed keratin peptides and plant-derived hydrolyzed polypeptides, the pH of the standardized plant active ingredient pre-dispersion solution is adjusted to 5.2-5.8. After adding hydrolyzed keratin peptides and plant-derived hydrolyzed polypeptides, the mixture is stirred and compounded at 25-35°C for 30-50 minutes. In step S3, after adding β-glucan and sodium hyaluronate, the mixture is stirred and stabilized for 10-30 minutes.

[0014] More preferably, in step S2, the hydrolyzed keratin peptide and plant-derived hydrolyzed polypeptide are added in 2 to 4 batches, with an interval of 5 to 10 minutes between adjacent batches; in step S3, the β-glucan and sodium hyaluronate are pre-wetted with deionized water and then added to the polypeptide-plant active ingredient pre-complex solution; in step S5, the stabilized polypeptide-plant active ingredient pre-complex solution is added when the temperature of the formulation matrix is ​​not higher than 40°C, and the pH of the resulting formulation is 5.0 to 6.0.

[0015] The beneficial effects of this invention are: This invention controls the characteristic active ingredients of licorice extract, scutellaria baicalensis extract, and rosemary extract, and pre-complexes them with hydrolyzed keratin peptides and plant-derived hydrolyzed polypeptides in a weakly acidic polyol aqueous system. The pre-complex is then stabilized using β-glucan and sodium hyaluronate to form a stabilized polypeptide-plant active ingredient pre-complex solution. This pre-complex system allows for a relatively stable dispersion of plant active ingredients and low-molecular-weight polypeptides through intermolecular interactions such as hydrogen bonding, electrostatic adsorption, and hydrophobic association, reducing precipitation, discoloration, and activity fluctuations of plant active ingredients during storage. Hydrolyzed keratin peptides have good affinity for hair and keratin surfaces; combined with plant-derived hydrolyzed polypeptides, they improve the adhesion and retention of active ingredients on the scalp and hair surface, reducing the loss of effective ingredients after rinsing or wiping. β-glucan and sodium hyaluronate further enhance the aqueous stability and gentleness of the system. The resulting formulation exhibits good dispersion stability, scalp compatibility, and antibacterial long-lasting effect, and can play a comprehensive care role in relieving scalp discomfort, maintaining scalp microecology, reducing hair loss during combing, and enhancing hair root stability. Attached Figure Description

[0016] The invention will now be further described with reference to the accompanying drawings.

[0017] Figure 1 This is a comparison chart showing the overall retention rate of active ingredients in the formulations obtained in Examples 1-3 and Comparative Examples 1-3; Figure 2 Comparison chart of the overall residual rates after elution of the formulations obtained in Examples 1-3 and Comparative Examples 1-3. Detailed Implementation

[0018] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0019] In the following examples and comparative examples, unless otherwise stated, all raw materials used are commercially available cosmetic grade raw materials, all water used is deionized water, and all parts are parts by weight.

[0020] The licorice extract, scutellaria baicalensis extract, and rosemary extract are all commercially available cosmetic-grade plant extracts that can be uniformly dispersed in a polyol aqueous phase system composed of glycerol, 1,3-propanediol, and water. Based on the mass of the corresponding extracts, the licorice extract contains 5%–20% glycyrrhizic acid, the scutellaria baicalensis extract contains 10%–40% baicalin, and the rosemary extract contains 2%–10% rosmarinic acid. In the following examples and comparative examples, the licorice extract used contained 10% glycyrrhizic acid, the scutellaria baicalensis extract contained 20% baicalin, and the rosemary extract contained 5% rosmarinic acid. The hydrolyzed keratin peptide is a low-molecular-weight peptide raw material obtained by hydrolysis or enzymatic hydrolysis of keratin-derived substances, and the plant-derived hydrolyzed polypeptide is a low-molecular-weight peptide raw material obtained by hydrolysis or enzymatic hydrolysis of soybean protein, wheat protein, pea protein or oat protein; the molecular weight of both the hydrolyzed keratin peptide and the plant-derived hydrolyzed polypeptide is 300-3000 Da, and the peptide content is not less than 80%.

[0021] Example 1: This example provides a soothing, antibacterial, anti-hair loss, and hair-strengthening preparation of a polypeptide synergistic with plant active ingredients. The preparation method includes the following steps: S1. Weigh 1.0 part of licorice extract, 1.0 part of scutellaria extract, 0.5 part of rosemary extract, 10 parts of glycerin, 6 parts of 1,3-propanediol and 76.78 parts of deionized water (part 1). Add the licorice extract, scutellaria extract and rosemary extract to the glycerin, 1,3-propanediol and deionized water (part 1), and stir and disperse at 25°C for 20 min to obtain a standardized pre-dispersion of plant active ingredients.

[0022] S2. Add the first part of the pH adjuster to the standardized plant active ingredient pre-dispersion obtained in step S1 to adjust the pH of the system to 5.2; then weigh 1.5 parts of hydrolyzed keratin peptide and 1.0 parts of hydrolyzed soybean protein peptide, add the hydrolyzed keratin peptide and hydrolyzed soybean protein peptide in two batches, with an interval of 5 minutes between the two batches. After all the peptides are added, continue to stir and compound at 25°C for 30 minutes to obtain the peptide-plant active ingredient pre-composite solution.

[0023] S3. Weigh 0.2 parts of β-glucan, 0.02 parts of sodium hyaluronate and 2.00 parts of the second part of deionized water. After pre-wetting β-glucan and sodium hyaluronate with the second part of deionized water, add them to the polypeptide-plant active ingredient precomplex solution obtained in step S2. Stir and stabilize at 25°C for 10 min to obtain the stabilized polypeptide-plant active ingredient precomplex solution.

[0024] S4. Weigh 0.1 parts of hydroxyethyl cellulose and add it to 75.00 parts of deionized water. Stir to disperse and fully hydrate to obtain a thickened aqueous phase. Then weigh 3 parts of betaine, 0.3 parts of panthenol, 0.2 parts of allantoin, 0.25 parts of phenoxyethanol and 0.05 parts of ethylhexylglycerin and add them to the thickened aqueous phase. Stir evenly to obtain the formulation matrix.

[0025] S5. When the temperature of the formulation matrix obtained in step S4 is not higher than 40℃, weigh 15 parts of the stabilized polypeptide-plant active ingredient pre-composite solution obtained in step S3, add it to the formulation matrix, and stir evenly; then add the second part of pH adjuster to adjust the pH of the system to 5.0, and add 6.05 parts of deionized water to 100 parts, and continue stirring until the system is uniform, thus obtaining the soothing, antibacterial, anti-hair loss and hair strengthening formulation of polypeptide synergistic with plant active ingredients.

[0026] In step S2 and step S5, the pH adjuster is composed of citric acid and arginine. The amount of pH adjuster used is based on adjusting the system to the target pH, and the total amount used in the final formulation is 0.05 parts.

[0027] Example 2: This example provides a soothing, antibacterial, anti-hair loss, and hair-strengthening preparation of a polypeptide synergistic with plant active ingredients. The preparation method includes the following steps: S1. Weigh 3.5 parts of licorice extract, 4.0 parts of scutellaria extract, 2.5 parts of rosemary extract, 25 parts of glycerin, 18 parts of 1,3-propanediol and 32.50 parts of deionized water (part 1). Add the licorice extract, scutellaria extract and rosemary extract to the glycerin, 1,3-propanediol and deionized water (part 1), and stir and disperse at 35°C for 30 min to obtain a standardized pre-dispersion of plant active ingredients.

[0028] S2. Add the first part of the pH adjuster to the standardized plant active ingredient predispersant obtained in step S1 to adjust the pH of the system to 5.8; then weigh 4.5 parts of hydrolyzed keratin peptide and 3.5 parts of hydrolyzed soybean protein peptide, add the hydrolyzed keratin peptide and hydrolyzed soybean protein peptide in 4 batches, with an interval of 10 min between adjacent batches. After all the peptides are added, continue to stir and compound at 35℃ for 50 min to obtain the peptide-plant active ingredient precomposite solution.

[0029] S3. Weigh 2.0 parts of β-glucan, 0.5 parts of sodium hyaluronate and 4.00 parts of the second part of deionized water. After pre-wetting β-glucan and sodium hyaluronate with the second part of deionized water, add them to the polypeptide-plant active ingredient precomplex solution obtained in step S2. Stir and stabilize at 35°C for 30 min to obtain the stabilized polypeptide-plant active ingredient precomplex solution.

[0030] S4. Weigh 0.3 parts of hydroxyethyl cellulose and 0.3 parts of xanthan gum, add them to 45.00 parts of deionized water (part III), stir to disperse and fully hydrate to obtain a thickened aqueous phase; then weigh 4 parts of glycerol, 4 parts of 1,3-propanediol, 2 parts of betaine, 1 part of panthenol, 1 part of nicotinamide, 1 part of sodium pyrrolidone carboxylate, 0.8 parts of phenoxyethanol and 0.2 parts of ethylhexylglycerol, add them to the thickened aqueous phase, stir evenly to obtain the formulation matrix.

[0031] S5. When the temperature of the formulation matrix obtained in step S4 is not higher than 40℃, weigh 35 parts of the stabilized polypeptide-plant active ingredient pre-composite solution obtained in step S3, add it to the formulation matrix, and stir evenly; then add the second part of pH adjuster to adjust the pH of the system to 6.0, and add 4.90 parts of deionized water to 100 parts, and continue stirring until the system is uniform, thus obtaining the soothing, antibacterial, anti-hair loss and hair strengthening formulation of polypeptide synergistic with plant active ingredients.

[0032] In step S2 and step S5, the pH adjuster is composed of citric acid and arginine, and the total amount of the first part pH adjuster and the second part pH adjuster is 0.5 parts.

[0033] Example 3: This example provides a soothing, antibacterial, anti-hair loss, and hair-strengthening preparation of a polypeptide synergistic with plant active ingredients. The preparation method includes the following steps: S1. Weigh 2.2 parts of licorice extract, 2.5 parts of scutellaria extract, 1.5 parts of rosemary extract, 16 parts of glycerin, 10 parts of 1,3-propanediol and 58.40 parts of deionized water (part 1). Add the licorice extract, scutellaria extract and rosemary extract to the glycerin, 1,3-propanediol and deionized water (part 1), and stir and disperse at 30°C for 25 min to obtain a standardized pre-dispersion of plant active ingredients.

[0034] S2. Add the first part of the pH adjuster to the standardized plant active ingredient predispersant obtained in step S1 to adjust the pH of the system to 5.5; then weigh 3.0 parts of hydrolyzed keratin peptide and 2.2 parts of hydrolyzed soybean protein peptide, add the hydrolyzed keratin peptide and hydrolyzed soybean protein peptide in 3 batches, with an interval of 8 minutes between two adjacent batches. After all the peptides are added, continue to stir and compound at 30°C for 40 minutes to obtain the peptide-plant active ingredient precomposite solution.

[0035] S3. Weigh 1.0 part of β-glucan, 0.2 part of sodium hyaluronate and 3.00 part of the second part of deionized water. After pre-wetting β-glucan and sodium hyaluronate with the second part of deionized water, add them to the polypeptide-plant active ingredient precomplex solution obtained in step S2. Stir and stabilize at 30°C for 20 min to obtain the stabilized polypeptide-plant active ingredient precomplex solution.

[0036] S4. Weigh 0.2 parts of hydroxyethyl cellulose and 0.1 parts of xanthan gum, add them to 60.00 parts of deionized water (part III), stir to disperse and fully hydrate to obtain a thickened aqueous phase; then weigh 2 parts of glycerol, 2 parts of 1,3-propanediol, 2 parts of betaine, 0.5 parts of panthenol, 0.2 parts of allantoin, 0.8 parts of sodium pyrrolidone carboxylate, 0.6 parts of phenoxyethanol and 0.1 parts of ethylhexylglycerol, add them to the thickened aqueous phase, stir evenly to obtain the formulation matrix.

[0037] S5. When the temperature of the formulation matrix obtained in step S4 is not higher than 40℃, weigh 25 parts of the stabilized polypeptide-plant active ingredient pre-composite solution obtained in step S3, add it to the formulation matrix, and stir evenly; then add the second part of pH adjuster to adjust the pH of the system to 5.6, and add 6.30 parts of deionized water to 100 parts, and continue stirring until the system is uniform, thus obtaining the soothing, antibacterial, anti-hair loss and hair strengthening formulation of polypeptide synergistic with plant active ingredients.

[0038] In step S2 and step S5, the pH adjuster is composed of citric acid and arginine, and the total amount of the first part pH adjuster and the second part pH adjuster is 0.2 parts.

[0039] Comparative Example 1: This comparative example provides a soothing, antibacterial, anti-hair loss, and hair-strengthening preparation with the synergistic effect of peptides and plant active ingredients. This comparative example does not perform pre-dispersion of plant active ingredients, pre-compounding of peptides, or pre-compounding stabilization treatment of β-glucan and sodium hyaluronate. Instead, each active component is directly added to the preparation matrix. S1. Weigh 0.2 parts of hydroxyethyl cellulose and 0.1 parts of xanthan gum, add them to 75.00 parts of deionized water, stir to disperse and fully hydrate to obtain a thickened aqueous phase.

[0040] S2. Weigh 6 parts glycerol, 4.5 parts 1,3-propanediol, 2 parts betaine, 0.5 parts panthenol, 0.2 parts allantoin, 0.8 parts sodium pyrrolidone carboxylate, 0.6 parts phenoxyethanol and 0.1 parts ethylhexylglycerol, add them to the thickening aqueous phase obtained in step S1, stir evenly to obtain the formulation matrix.

[0041] S3. Weigh 0.55 parts of licorice extract, 0.625 parts of scutellaria extract, 0.375 parts of rosemary extract, 0.75 parts of hydrolyzed keratin peptide and 0.55 parts of hydrolyzed soybean protein peptide, and add them directly to the formulation matrix obtained in step S2. Stir at 30°C for 40 minutes to ensure that the components are evenly dispersed.

[0042] S4. Weigh 0.25 parts of β-glucan and 0.05 parts of sodium hyaluronate, add them directly to the system obtained in step S3, and continue stirring for 20 minutes to disperse them evenly.

[0043] S5. Add a pH adjuster to adjust the pH of the system to 5.6. The pH adjuster is composed of citric acid and arginine, and the amount of pH adjuster is 0.2 parts. Then add 6.65 parts of deionized water to 100 parts and continue stirring until the system is uniform to obtain the preparation of Comparative Example 1.

[0044] Comparative Example 2: In this comparative example, hydrolyzed keratin peptides were not added to the stabilized polypeptide-plant active ingredient pre-complex solution, and the 3.0 parts of hydrolyzed keratin peptides in Example 3 were replaced with an equal amount of hydrolyzed soybean protein peptides.

[0045] S1. Weigh 2.2 parts of licorice extract, 2.5 parts of scutellaria extract, 1.5 parts of rosemary extract, 16 parts of glycerin, 10 parts of 1,3-propanediol and 58.40 parts of deionized water (part 1). Add the licorice extract, scutellaria extract and rosemary extract to the glycerin, 1,3-propanediol and deionized water (part 1), and stir and disperse at 30°C for 25 min to obtain a standardized pre-dispersion of plant active ingredients.

[0046] S2. Add the first part of the pH adjuster to the standardized plant active ingredient pre-dispersion obtained in step S1 to adjust the pH of the system to 5.5; then weigh 5.2 parts of hydrolyzed soybean protein peptide, add the hydrolyzed soybean protein peptide in 3 batches, with an interval of 8 minutes between two adjacent batches, and continue to stir and compound at 30℃ for 40 minutes after all the peptides are added to obtain a polypeptide-plant active ingredient pre-composite solution without hydrolyzed keratin peptide.

[0047] S3. Weigh 1.0 part of β-glucan, 0.2 part of sodium hyaluronate and 3.00 part of the second part of deionized water. After pre-wetting β-glucan and sodium hyaluronate with the second part of deionized water, add them to the polypeptide-plant active ingredient precomplex solution obtained in step S2 that does not contain hydrolyzed keratin peptides. Stir and stabilize at 30°C for 20 min to obtain a stabilized polypeptide-plant active ingredient precomplex solution that does not contain hydrolyzed keratin peptides.

[0048] S4. Weigh 0.2 parts of hydroxyethyl cellulose and 0.1 parts of xanthan gum, add them to 60.00 parts of deionized water (part III), stir to disperse and fully hydrate to obtain a thickened aqueous phase; then weigh 2 parts of glycerol, 2 parts of 1,3-propanediol, 2 parts of betaine, 0.5 parts of panthenol, 0.2 parts of allantoin, 0.8 parts of sodium pyrrolidone carboxylate, 0.6 parts of phenoxyethanol and 0.1 parts of ethylhexylglycerol, add them to the thickened aqueous phase, stir evenly to obtain the formulation matrix.

[0049] S5. When the temperature of the formulation matrix obtained in step S4 is not higher than 40℃, weigh 25 parts of the stabilized polypeptide-plant active ingredient pre-composite liquid without hydrolyzed keratin peptides obtained in step S3, add it to the formulation matrix, and stir evenly; then add the second part of pH adjuster to adjust the pH of the system to 5.6, and add 6.30 parts of deionized water to 100 parts, and continue stirring until the system is uniform, thus obtaining the formulation of Comparative Example 2.

[0050] In step S2 and step S5, the pH adjuster is composed of citric acid and arginine, and the total amount of the first part pH adjuster and the second part pH adjuster is 0.2 parts.

[0051] Comparative Example 3: In this comparative example, β-glucan and sodium hyaluronate were not added in the peptide-plant active ingredient pre-complex stage, but were added directly to the formulation matrix.

[0052] S1. Weigh 2.2 parts of licorice extract, 2.5 parts of scutellaria extract, 1.5 parts of rosemary extract, 16 parts of glycerin, 10 parts of 1,3-propanediol and 58.40 parts of deionized water (part 1). Add the licorice extract, scutellaria extract and rosemary extract to the glycerin, 1,3-propanediol and deionized water (part 1), and stir and disperse at 30°C for 25 min to obtain a standardized pre-dispersion of plant active ingredients.

[0053] S2. Add the first part of the pH adjuster to the standardized plant active ingredient predispersant obtained in step S1 to adjust the pH of the system to 5.5; then weigh 3.0 parts of hydrolyzed keratin peptide and 2.2 parts of hydrolyzed soybean protein peptide, add the hydrolyzed keratin peptide and hydrolyzed soybean protein peptide in 3 batches, with an interval of 8 minutes between two adjacent batches. After all the peptides are added, continue to stir and compound at 30°C for 40 minutes to obtain the peptide-plant active ingredient precomposite solution.

[0054] S3. Weigh 4.20 parts of the second part of deionized water and add it to the polypeptide-plant active ingredient precomplex solution obtained in step S2. Continue stirring at 30°C for 20 minutes to obtain the polypeptide-plant active ingredient precomplex solution without added stabilized polysaccharides.

[0055] S4. Weigh 0.2 parts of hydroxyethyl cellulose and 0.1 parts of xanthan gum, add them to 60.00 parts of deionized water (part III), stir to disperse and fully hydrate to obtain a thickened aqueous phase; then weigh 0.25 parts of β-glucan, 0.05 parts of sodium hyaluronate, 2 parts of glycerol, 2 parts of 1,3-propanediol, 2 parts of betaine, 0.5 parts of panthenol, 0.2 parts of allantoin, 0.8 parts of sodium pyrrolidone carboxylate, 0.6 parts of phenoxyethanol and 0.1 parts of ethylhexylglycerin, add them to the thickened aqueous phase, stir evenly to obtain the formulation matrix.

[0056] S5. When the temperature of the formulation matrix obtained in step S4 is not higher than 40℃, weigh 25 parts of the unstabilized polysaccharide polypeptide-plant active ingredient precomposite solution obtained in step S3, add it to the formulation matrix, and stir evenly; then add the second part of pH adjuster to adjust the pH of the system to 5.6, and add 6.00 parts of deionized water to 100 parts, and continue stirring until the system is uniform to obtain the formulation of Comparative Example 3.

[0057] In step S2 and step S5, the pH adjuster is composed of citric acid and arginine, and the total amount of the first part pH adjuster and the second part pH adjuster is 0.2 parts.

[0058] Performance testing To verify the technical effects of the present invention, performance tests were conducted on the formulations obtained in Examples 1-3 and Comparative Examples 1-3. All samples were equilibrated at 25°C for 24 hours before testing.

[0059] (1) Stability test: The test method of heat resistance, cold resistance and centrifugation test in GB / T 29665-2013 "Skin Care Lotion" was followed. Each sample was subjected to heat resistance at 40±1℃ for 24h, cold resistance at -8±2℃ for 24h and centrifugation at 2000r / min for 30min. After the treatment, the samples were restored to room temperature and the presence of stratification, precipitation, flocculation or obvious color change was observed. The samples were scored according to the appearance changes. 0 points indicated no stratification, no precipitation or flocculation, 1 point indicated slight turbidity or slight precipitation, 2 points indicated visible precipitation or flocculation, and 3 points indicated obvious stratification or obvious precipitation.

[0060] (2) Active ingredient retention rate test: Each sample was placed at a constant temperature of 45℃ for 30 days. Samples were taken on day 0 and day 30 respectively. The contents of glycyrrhizic acid, baicalin and rosmarinic acid in the sample were determined by high performance liquid chromatography. The active ingredient retention rate was calculated according to "content on day 30 / content on day 0 × 100%". The average value of the retention rates of the three characteristic active ingredients was recorded as the comprehensive active ingredient retention rate.

[0061] (3) Keratin binding rate and residual rate after elution test: Cleaned and dried human hair bundles were used as the keratin substrate for simulating hair. In the keratin binding rate test, an equal volume of sample dilution was contacted with human hair bundles at 32°C for 30 min. After separating the supernatant, the residual contents of glycyrrhizic acid, baicalin, and rosmarinic acid were determined by high performance liquid chromatography. The keratin binding rate of each active ingredient was calculated according to "keratin binding rate = (initial active ingredient content - residual active ingredient content in supernatant) / initial active ingredient content × 100%". The average value of the binding rates of the three marker active ingredients was recorded as the comprehensive keratin binding rate. In the residual rate test after elution, an equal volume of sample was coated on the surface of human hair bundles. After standing for 30 min, it was rinsed with a fixed volume of deionized water. After drying, the residual active ingredients on the surface of the hair bundles were extracted, and the contents of glycyrrhizic acid, baicalin, and rosmarinic acid were determined by high performance liquid chromatography. The average value of the residual rates of the three marker active ingredients was recorded as the comprehensive residual rate after elution.

[0062] (4) Antibacterial efficacy test: The test was conducted in accordance with QB / T 2738-2023 "Evaluation Method for Antibacterial and Antimicrobial Effects of Daily Chemical Products" and combined with simulated rinsing treatment. Staphylococcus aureus and Escherichia coli were selected as test bacteria. Each sample was coated on the surface of a keratin simulated substrate, left to stand for 30 minutes, then subjected to simulated rinsing, and then left to stand for 4 hours before the antibacterial rate was measured.

[0063] The results are shown in Tables 1 and 2 below.

[0064] Table 1. Stability and active ingredient retention test results of the examples and comparative examples.

[0065] Table 2. Results of keratin binding rate, elution residue rate, and antibacterial persistence tests for the examples and comparative examples.

[0066] As shown in Tables 1 and 2, Examples 1-3 are superior to the comparative examples in terms of stability, overall active ingredient retention rate, overall keratin binding rate, overall residue rate after elution, and antibacterial rate after simulated rinsing. This indicates that the stabilized polypeptide-plant active ingredient pre-complex system constructed in this invention can effectively exert a synergistic effect. Compared with Comparative Example 1, which was directly mixed, the sample of the examples did not show obvious precipitation or flocculation, and the active ingredient retention rate was significantly improved, indicating that the dispersion state and storage stability of plant active ingredients were improved after pre-dispersion of licorice extract, scutellaria baicalensis extract, and rosemary extract in a polyol aqueous phase and pre-complexation with polypeptides. Compared with Comparative Example 2, the keratin binding rate and residue rate after elution of the examples were significantly increased, indicating that hydrolyzed keratin peptides can enhance the affinity of the pre-complex system to the surface of hair keratin. Compared with Comparative Example 3, the examples are superior in terms of stability and antibacterial persistence, indicating that β-glucan and sodium hyaluronate participate in the stabilization treatment during the pre-complexation stage, which can further enhance the stability of the system and the retention effect of active ingredients after use.

[0067] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0068] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed invention.

Claims

1. A soothing, antibacterial, anti-hair loss, and hair-strengthening preparation containing polypeptides and synergistic plant active ingredients, characterized in that, By weight, the mixture includes 15-35 parts of stabilized polypeptide-plant active ingredient pre-complex solution, 3-10 parts of matrix moisturizer, 0.5-3 parts of scalp conditioner, 0.1-0.6 parts of thickener, 0.05-0.5 parts of pH adjuster, 0.3-1.0 parts of preservative, and deionized water to make up to 100 parts. The stabilized polypeptide-plant active ingredient pre-composite solution is prepared by pre-dispersion, pre-composite, and stabilization treatment of standardized plant active ingredients, low molecular weight polypeptide combination, polyol aqueous phase system, and stabilized polysaccharide; the low molecular weight polypeptide combination consists of hydrolyzed keratin peptides and plant-derived hydrolyzed polypeptides, and the molecular weight of both hydrolyzed keratin peptides and plant-derived hydrolyzed polypeptides is 300-3000 Da, and the peptide content is not less than 80%; the standardized plant active ingredients are licorice extract, scutellaria baicalensis extract, and rosemary extract; the low molecular weight polypeptide combination consists of hydrolyzed keratin peptides and plant-derived hydrolyzed polypeptides; the polyol aqueous phase system consists of glycerol, 1,3-propanediol, and deionized water; the stabilized polysaccharide includes β-glucan and sodium hyaluronate.

2. The soothing, antibacterial, anti-hair loss, and hair-strengthening preparation of peptides and plant active ingredients according to claim 1, characterized in that, Based on 100 parts of the stabilized polypeptide-plant active ingredient pre-complex solution, the stabilized polypeptide-plant active ingredient pre-complex solution includes 1.0-3.5 parts of licorice extract, 1.0-4.0 parts of scutellaria baicalensis extract, 0.5-2.5 parts of rosemary extract, 1.5-4.5 parts of hydrolyzed keratin peptide, 1.0-3.5 parts of plant-derived hydrolyzed polypeptide, 0.2-2.0 parts of β-glucan, 0.02-0.5 parts of sodium hyaluronate, 10-25 parts of glycerin, 6-18 parts of 1,3-propanediol, and deionized water to make up to 100 parts.

3. The soothing, antibacterial, anti-hair loss, and hair-strengthening preparation of peptides and plant active ingredients according to claim 1, characterized in that, Based on the mass of the corresponding extracts, the licorice extract contains 5% to 20% glycyrrhizic acid, the scutellaria extract contains 10% to 40% baicalin, and the rosemary extract contains 2% to 10% rosmarinic acid; in the stabilized polypeptide-plant active ingredient pre-complex solution, the mass ratio of glycyrrhizic acid, baicalin, and rosmarinic acid is 1:(0.8 to 2.5):(0.2 to 1.0).

4. The soothing, antibacterial, anti-hair loss, and hair-strengthening preparation of peptides and plant active ingredients according to claim 1, characterized in that, In the low molecular weight polypeptide combination, the mass ratio of the hydrolyzed keratin peptide to the plant-derived hydrolyzed polypeptide is 1:(0.5-2.0) based on dry weight.

5. The soothing, antibacterial, anti-hair loss, and hair-strengthening preparation of peptides and plant active ingredients according to claim 1, characterized in that, The plant-derived hydrolyzed polypeptide is one or more of hydrolyzed soybean protein peptide, hydrolyzed pea protein peptide, hydrolyzed oat protein peptide, and hydrolyzed wheat protein peptide.

6. The soothing, antibacterial, anti-hair loss, and hair-strengthening preparation of peptides and synergistic plant active ingredients according to claim 1, characterized in that, The mass ratio of β-glucan to sodium hyaluronate is 1:(0.05~0.5).

7. The soothing, antibacterial, anti-hair loss, and hair-strengthening preparation of peptides and plant active ingredients according to claim 1, characterized in that, The base moisturizer is one or more of glycerin, 1,3-propanediol, and betaine; the scalp conditioning agent is one or more of panthenol, nicotinamide, allantoin, and sodium pyrrolidone carboxylate; the thickener is one or more of hydroxyethyl cellulose, xanthan gum, and carbomer; the pH adjuster is one or more of citric acid, lactic acid, arginine, and sodium hydroxide; and the preservative is one or more of phenoxyethanol, ethylhexylglycerin, p-hydroxyacetophenone, and capryloyl hydroxamic acid.

8. A method for preparing a soothing, antibacterial, anti-hair loss, and hair-strengthening preparation of a polypeptide synergistic with plant active ingredients according to any one of claims 1 to 7, characterized in that, Includes the following steps: S1. Glycyrrhiza extract, Scutellaria baicalensis extract and rosemary extract are added to glycerol, 1,3-propanediol and the first part of deionized water, and stirred to disperse to obtain a standardized pre-dispersion of plant active ingredients; S2. Add the first part of the pH adjuster to the standardized plant active substance pre-dispersion solution to adjust the pH of the system, then add hydrolyzed keratin peptide and plant-derived hydrolyzed polypeptide, stir and combine to obtain polypeptide-plant active substance pre-composite solution; S3. After pre-wetting β-glucan and sodium hyaluronate with the second part of deionized water, add them to the polypeptide-plant active ingredient precomplex solution, stir to stabilize, and obtain a stabilized polypeptide-plant active ingredient precomplex solution. S4. Add the thickener to the deionized water in the third part, disperse and hydrate it, then add the matrix moisturizer, scalp conditioner and preservative, stir evenly to obtain the formulation matrix; S5. Add the stabilized polypeptide-plant active ingredient pre-complex solution obtained in step S3 to the formulation matrix, stir evenly, and add the second part of pH adjuster to adjust the pH of the system to obtain the soothing, antibacterial, anti-hair loss and hair strengthening formulation of polypeptide synergistic with plant active ingredients.

9. The preparation method according to claim 8, characterized in that, In step S2, before adding hydrolyzed keratin peptides and plant-derived hydrolyzed polypeptides, the pH of the standardized plant active ingredient pre-dispersion solution is adjusted to 5.2-5.

8. After adding hydrolyzed keratin peptides and plant-derived hydrolyzed polypeptides, the mixture is stirred and compounded at 25-35°C for 30-50 min. In step S3, after adding β-glucan and sodium hyaluronate, the mixture is stirred and stabilized for 10-30 min.

10. The preparation method according to claim 8, characterized in that, In step S2, the hydrolyzed keratin peptides and plant-derived hydrolyzed polypeptides are added in 2 to 4 batches, with an interval of 5 to 10 minutes between adjacent batches; in step S3, the β-glucan and sodium hyaluronate are pre-wetted with deionized water and then added to the polypeptide-plant active ingredient pre-complex solution; in step S5, the stabilized polypeptide-plant active ingredient pre-complex solution is added when the temperature of the formulation matrix is ​​not higher than 40°C, and the pH of the resulting formulation is 5.0 to 6.0.

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