A composition having a skin barrier repair effect and use thereof

Abstract

The present application belongs to the technical field of cosmetics, and particularly relates to a composition with skin barrier repairing effect, a cosmetic and application thereof. Specifically, the present application combines recombinant humanized collagen, extract of cucurbita pepo, and extract of acanthopanax, and the components synergize after compounding, can significantly improve the expression of desmoglein 1 (DSG1), F-actin and ceramide, so as to achieve the effect of repairing skin barrier. Then, the composition is prepared into a skin care cosmetic as the main effective component, and the test verification has the functions of significantly improving skin redness, reducing water flow, and improving skin water content.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a composition that repairs the skin barrier and its application in cosmetic formulations. Background Technology

[0002] The information disclosed in the background section of this invention is intended only to enhance the understanding of the overall background of the invention and is not necessarily to be construed as an admission or in any way implying that such information constitutes prior art known to those skilled in the art.

[0003] Skin wound repair is an extremely complex biological process involving the orderly interaction of various cell types, extracellular matrix, and cytokines in time and space, ultimately leading to skin barrier repair and maintenance of tissue homeostasis. Therefore, promoting the integrity of the skin barrier is of paramount importance.

[0004] Desmosomes are the main structures connecting keratinocytes. Desmosome core glycoprotein 1 (DSG1) is the main cadherin protein in desmosomes, regulating epidermal differentiation through interactions with desmosome proteins such as Erbin and Plakoglobin. DSG1 deficiency or impaired function leads to skin barrier damage. Actin filaments (F-actin) are a core component of the cytoskeleton, providing mechanical support to cells through assembly with various actin-binding proteins (ABPs). External stimuli are transmitted to the F-actin cortex via receptors such as integrins, stimulating sensitive intracellular signaling pathways. This inhibits F-actin polymerization, leading to decreased cell migration. Intercellular lipids are structural lipids distributed in the intercellular spaces of the stratum corneum of the epidermis, mainly composed of ceramides, cholesterol, free fatty acids, and small amounts of phospholipids. They maintain the skin's permeability barrier function by forming a "brick-and-mortar" structure with keratinocytes.

[0005] Recombinant humanized collagen boasts excellent bioactivity, significantly shortening wound healing time and reducing scarring. It is a novel, high-end repair material used in wound healing, tissue repair, and skin barrier repair, greatly enhancing skin barrier function in individuals with trauma, post-medical aesthetic procedures, and sensitive skin, thus meeting urgent market demand. However, the current market price of recombinant humanized collagen is high, limiting its application in skincare products.

[0006] The inventors have discovered that by combining recombinant collagen with plant-based ingredients, the barrier repair capability of recombinant collagen can be greatly improved, thus saving on product formulation costs. Summary of the Invention

[0007] To address the shortcomings of existing technologies, this invention provides a composition for repairing the skin barrier and its application in cosmetic formulations. Specifically, this invention uses recombinant humanized collagen (referred to as collagen), Trichosanthes kirilowii extract, and Acanthopanax senticosus extract to prepare a composition; then, using this composition as the main active ingredient, a cosmetic product that promotes the integrity of the skin barrier is prepared. Experimental verification has shown that it has excellent skin barrier repairing effects. Based on the above research results, this invention is thus completed.

[0008] To achieve the above-mentioned technical objectives, the present invention provides the following technical solution:

[0009] A first aspect of the present invention provides a composition for repairing the skin barrier, the composition comprising the following components in parts by weight:

[0010] 0.01-1.0 parts of recombinant humanized collagen, 1.0-15.0 parts of Trichosanthes kirilowii extract, and 1.0-15.0 parts of Acanthopanax senticosus extract;

[0011] Furthermore, the composition for repairing the skin barrier comprises the following components in parts by weight:

[0012] 0.1-0.5 parts of recombinant humanized collagen, 5.0-10.0 parts of Trichosanthes kirilowii extract, and 5.0-10.0 parts of Acanthopanax senticosus extract.

[0013] The recombinant humanized collagen is from Shandong Freda Biotechnology Co., Ltd. (molecular weight approximately 70,000 Da).

[0014] The *Trichosanthes kirilowii* extract is obtained from dried *Trichosanthes kirilowii* powder through steps such as alcohol extraction and concentration. A preferred method is as follows: Add dried *Trichosanthes kirilowii* powder to a 60%–80% ethanol solution, extract 2–4 times in a water bath at 60–80°C, combine the filtrates, concentrate under reduced pressure, evaporate until no alcohol odor remains, add 0.8–1.2 times the mass of butanediol, stir well, and filter to obtain the *Trichosanthes kirilowii* extract. The concentration, based on the crude drug, is 0.40–0.6 g / mL, meaning 0.40–0.6 g of dried *Trichosanthes kirilowii* extract powder yields 1 mL of *Trichosanthes kirilowii* extract (solution). It can also be obtained commercially available.

[0015] The Acanthopanax senticosus extract was obtained using pulsed ultrasound-assisted subcritical extraction. Specifically, dried Acanthopanax senticosus was pulverized and sieved, then water was added for pulsed ultrasound extraction at room temperature. Water was then added further, and subcritical extraction was performed at 120–140°C and 0.8–1.2 MPa. The filtrate was concentrated under reduced pressure to 4–6 times the mass of the Acanthopanax senticosus powder. Glycerin was added in an amount 1–1.5 times the volume of the concentrate, and the mixture was stirred until homogeneous. After filtration, the Acanthopanax senticosus extract was obtained, with a concentration of 0.3–1.0 g / mL (based on crude drug), meaning 0.3–1.0 g of dried Acanthopanax senticosus powder yielded 1 mL of Acanthopanax senticosus extract (solution). It can also be obtained commercially available.

[0016] Through experimental verification, the present invention demonstrates that the above composition can repair the skin barrier by increasing the expression levels of desmosome core glycoprotein 1 (DSG1), actin filaments (F-actin), and ceramide.

[0017] Therefore, a second aspect of the present invention provides the use of the above-described skin barrier repair composition in the preparation of skin care formulations. Obviously, the skin care formulations have effects such as enhancing the skin barrier and moisturizing.

[0018] A third aspect of the present invention provides a skin care preparation product having the effects of enhancing the skin barrier and moisturizing, wherein the skin care preparation product comprises at least the above-mentioned skin barrier repair composition.

[0019] According to the skin care formulation product of the present invention, the amount of the skin barrier repair composition added is 1-35% (w / w) based on the total mass of the skin care formulation product, such as 1%, 3%, 5%, 7%, 10%, 15%, 17%, 20%, 25%, 30%, 35%, etc., without specific limitation herein.

[0020] The skin care formulation may also contain any other raw materials permitted in the cosmetics field, including but not limited to emulsifiers, emollients, moisturizers, cooling agents, skin conditioning agents, thickeners, and preservatives.

[0021] Furthermore, by rationally adding the above-mentioned raw material components, this invention can also be used to prepare different cosmetic formulations, such as essence water, essence lotion, essence serum, shampoo, conditioner, hair mask, etc., without making specific limitations here.

[0022] The beneficial technical effects of one or more of the above technical solutions are as follows:

[0023] (1) The composition with skin barrier repairing effect obtained by the above technical solution can significantly increase the expression of desmosome core glycoprotein 1 (DSG1), actin filament (F-actin) and ceramide, thereby achieving the effect of repairing skin barrier;

[0024] (2) Recombinant humanized collagen is expensive. This invention combines recombinant humanized collagen, Trichosanthes kirilowii seed extract and Acanthopanax senticosus extract. When achieving similar or more significant effects in promoting skin barrier repair and moisturizing, the amount of recombinant humanized collagen used is lower, which greatly saves costs.

[0025] (3) The preparation method of the skin care preparation with the skin barrier repairing composition obtained by the above technical solution is simple and easy to implement, and saves costs. Through human efficacy test, it has good effects of promoting skin barrier repair and moisturizing, and therefore has good practical application value. Detailed Implementation

[0026] It should be noted that the following detailed description is illustrative and intended to provide further explanation of the invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0027] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of exemplary embodiments according to the invention. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0028] The present invention will now be further illustrated with specific examples. These examples are for illustrative purposes only and do not limit the scope of the invention. Unless otherwise specified, experimental conditions not explicitly stated in the examples are generally performed under conventional conditions or as recommended by the reagent company. Unless otherwise specified, all reagents and consumables used in the following examples are commercially available.

[0029] The following examples further illustrate the present invention, but do not constitute a limitation thereof. It should be understood that these examples are for illustrative purposes only and not for limiting the scope of the invention. Experimental methods not specifically described in the following examples are generally performed under conventional conditions. It should be noted that the recombinant humanized collagen (hereinafter referred to as "collagen") in the examples and comparative examples of the present invention was purchased from Shandong Freda Biotechnology Co., Ltd., with the trade name "Recombinant Collagen" and INCI name "Collagen," prepared according to the description in patent ZL202410290161.2.

[0030] The Trichosanthes kirilowii seed extract in the embodiments and comparative examples of this invention was obtained using the following method:

[0031] The dried Trichosanthes kirilowii seed powder was passed through a 150-mesh sieve. An appropriate amount of the powder was placed in a reflux jar, and 75% ethanol solution (volume fraction) was added at a material-to-liquid ratio of 1:7. The mixture was then refluxed in a water bath at 65°C for 1 hour. This extraction was repeated four times. The filtrates were combined, concentrated under reduced pressure, and evaporated until no alcohol odor was detected. Butanediol (1.5 times the mass of the concentrate) was added, stirred evenly, and filtered to obtain the Trichosanthes kirilowii seed extract. The extract concentration was 0.464 g / mL, calculated based on the crude drug, meaning 0.464 g of dried Trichosanthes kirilowii seed powder yielded 1 mL of Trichosanthes kirilowii seed extract (solution).

[0032] In the embodiments and comparative examples 1-8 of this invention, the Acanthopanax senticosus extract 1 was obtained by pulsed ultrasound-assisted subcritical extraction, and the specific method is as follows:

[0033] (1) After pulverizing the dried Acanthopanax senticosus, pass it through a 60-mesh sieve, add water and extract twice using pulsed ultrasound (working for 2 seconds and intermittently for 1 second). The material-to-liquid ratio is 1:10 each time, the power of the ultrasound is 400W, the temperature is 25℃, and each extraction lasts for 30 minutes. After the ultrasound is completed, filter the mixture, combine the filtrates, and set them aside for later use.

[0034] (2) Place the liquid prepared in step (1) into the extractor and continue to add water. The amount of water added is based on a mass ratio of Acanthopanax senticosus powder to water of 1:20 (the amount of water used for pulsed ultrasound is small, while the amount of water used for subcritical fluid extraction is large, so water needs to be added). The extraction conditions are: temperature of 140℃, pressure of 1.0MPa, and time of 40min. Filter the extract and concentrate the filtrate under reduced pressure to about 5 times the mass of Acanthopanax senticosus powder. Add 1.2 times the amount of glycerol to the concentrate, stir and mix evenly, and filter to obtain Acanthopanax senticosus extract. The concentration of the extract is 0.364g / mL (based on the amount of raw drug).

[0035] The Acanthopanax senticosus extract 2 was obtained by water extraction and alcohol precipitation, as follows: Dried Acanthopanax senticosus was pulverized and passed through a 60-mesh sieve. It was then extracted twice by reflux with water at a material-to-liquid ratio of 1:10, each extraction lasting 2 hours. The combined extracts were concentrated under reduced pressure to approximately three times the amount of raw material. An appropriate amount of 95% ethanol solution was added to the concentrate until the alcohol content reached 70% (w / w). The mixture was stirred for 10 minutes and refrigerated (4±2℃) for at least 12 hours. The solution was then filtered under reduced pressure using a sterile filter plate and a PP membrane. The ethanol in the filtrate was recovered under reduced pressure at 60℃, and the solution was concentrated until no alcohol odor remained. The solution was further concentrated to approximately three times the amount of raw material. Glycerin was added in an amount 1.3 times the volume of the concentrate, and the mixture was stirred until homogeneous before filtration to obtain the Acanthopanax senticosus extract. Based on the amount of raw material, the concentration of the Acanthopanax senticosus extract was 0.3013 g / mL.

[0036] Examples 1-3 and Comparative Examples 1-8:

[0037] The specific plan is shown in Table 1 below, calculated by mass parts. The preparation method is as follows: recombinant humanized collagen, Trichosanthes kirilowii seed extract and Acanthopanax senticosus extract are mixed according to the dosages of each embodiment and comparative ratio, and dissolved in purified water.

[0038] Table 1. List of components for specific embodiments and comparative examples.

[0039]

[0040] Note: Acanthopanax senticosus extract 1 was obtained by pulsed ultrasound-assisted subcritical extraction; Acanthopanax senticosus extract 2 was obtained by water extraction and alcohol precipitation.

[0041] Example 4: Skin Barrier Repair Essence

[0042] The above composition is used to prepare an essence product containing this skin barrier repair composition, and the formula is shown in Table 2.

[0043] Table 2. Formulas of Skin Barrier Repair Essence Products

[0044]

[0045] Preparation method of skin barrier repair serum:

[0046] S1: Add the raw materials in phase A separately, heat to 80~85℃ and stir to dissolve, and start cooling after all the raw materials have been dissolved;

[0047] S2: When the temperature drops to about 40~45℃, add phases B and C, stir and disperse evenly, and continue to cool down after dissolution is complete.

[0048] The preparation of Comparative Example 9 was the same as that of Example 4, except that Comparative Example 9 did not contain the composition of Example 2, and the other formulation components were the same.

[0049] Experiment 1: Detection of DSG1, F-actin and Ceramide Content

[0050] The experiment used UVB radiation to treat EpiKutis ® A 3D skin model was used for the study. According to the test groups in Table 3, drug administration was performed in groups, with three replicates per group. Except for the blank control (BC) group, all other groups received UVB irradiation at a dose of 600 mJ / cm². 2For groups BC and NC, 0.9 mL of model culture medium (EpiGrowth medium, Guangdong Boxi Shaanxi Branch) was added to each well. For group PC, 0.9 mL of culture medium containing the corresponding positive control was added to each well. For the sample groups, the corresponding concentration of working solution was evenly spread on the model surface. After irradiation, the 6-well plates were placed in an incubator (37℃, 5% CO2) and incubated for 24 hours. After incubation, the residual test material on the model surface was cleaned with sterile PBS (Solepro), and residual liquid inside and outside the model was wiped away with sterile cotton swabs.

[0051] Immunofluorescence detection: The fixation was performed with 4% paraformaldehyde (Biosharp) for 24 hours, followed by immunofluorescence detection.

[0052] Ceramide Assay: Lipid Sample Extraction—Place the model in a centrifuge tube, add proteinase K solution (Thermo, 17916), and incubate at 50°C for 2 hours. Use tweezers to peel off the viscous material from the stratum corneum, blot dry, and then place the stratum corneum into a glass tube. Add 1 mL of a chloroform-methanol mixture (chloroform to methanol volume ratio 1:1) to each glass tube, sonicate on ice, transfer the supernatant to a sample vial, and dry. Ceramide Detection—Add a mixture of acetonitrile and isopropanol (1:1) to the nitrogen-dried sample vial, centrifuge at 12000 rpm for 10 minutes, and transfer 100 μL of the upper layer to an inner liner tube for ceramide detection using LC-MS.

[0053]

[0054] Experimental results are expressed as Mean ± SD. Comparisons between groups were performed using... t -test Statistical analysis. All statistical analyses are two-tailed. 0.01 < P A value <0.05 is considered statistically significant. P A value <0.01 is considered highly significant. * indicates significance compared to the NC group. P- A value less than 0.05 is represented as *. P- A value less than 0.01 is represented as **.

[0055] Table 3 Test Plan

[0056]

[0057] Note: Sample concentration 5 mg / mL (diluted with deionized water).

[0058] Table 4 Summary of DSG1 Immunofluorescence Analysis Results

[0059]

[0060] (Note: ## indicates comparison with the blank control (BC))p <0.01; ** indicates a difference compared to the negative control (NC). p <0.01; * indicates that compared with the negative control (NC), 0.01 < p <0.05.

[0061] Table 4 shows that the DSG1 content in the PC group was significantly higher than that in the NC group, indicating that the positive control test was effective. Examples 1-3 all significantly upregulated DSG1 content, indicating that recombinant humanized collagen has a significant synergistic effect with Trichosanthes kirilowii seed extract and Acanthopanax senticosus extract 1. Single collagen also significantly promoted DSG1 expression, but the effect was not as pronounced as in Examples 1-3. Furthermore, the synergistic effect of recombinant humanized collagen with Acanthopanax senticosus extract 1 or Trichosanthes kirilowii seed extract was not significant. Acanthopanax senticosus extract 2 showed no significant upregulation effect on DSG1 compared to Acanthopanax senticosus extract 1, and Comparative Example 8 also indicates that recombinant humanized collagen did not have a synergistic effect with Acanthopanax senticosus extract 2 and Trichosanthes kirilowii seed extract.

[0062] Table 5 Summary of F-actin Immunofluorescence Analysis Results

[0063]

[0064] (Note: ## indicates comparison with the blank control (BC)) p <0.01; ** indicates a difference compared to the negative control (NC). p <0.01; * indicates that compared with the negative control (NC), 0.01 < p <0.05.

[0065] Table 5 shows that the F-actin content in the PC group was significantly higher than that in the NC group, indicating that the positive control test was effective. Examples 1-3 all significantly promoted F-actin expression, indicating that recombinant humanized collagen has a significant synergistic effect with Trichosanthes kirilowii seed extract and Acanthopanax senticosus extract 1. Single collagen also significantly promoted F-actin expression, but the effect was not as pronounced as in Examples 1-3, and the synergistic effect between collagen and Acanthopanax senticosus extract 1 or Trichosanthes kirilowii seed extract was not significant. Acanthopanax senticosus extract 2 alone did not promote F-actin expression, and Comparative Example 8 also shows that recombinant humanized collagen did not have a synergistic effect with Acanthopanax senticosus extract 2 and Trichosanthes kirilowii seed extract.

[0066] Table 6 Summary of Total Ceramide Concentration Detection Results

[0067]

[0068] (Note: ## indicates comparison with the blank control (BC)) p <0.01; ** indicates a difference compared to the negative control (NC). p <0.01; * indicates that compared with the negative control (NC), 0.01 < p <0.05.

[0069] Table 6 shows that the total ceramide concentration in the PC group was significantly higher than that in the NC group, indicating the effectiveness of this positive control test. Examples 1-3 all significantly promoted ceramide synthesis, indicating that collagen has a significant synergistic effect with Trichosanthes kirilowii seed extract and Acanthopanax senticosus extract 1. Trichosanthes kirilowii seed extract alone did not promote ceramide synthesis, and the synergistic effect between collagen and Trichosanthes kirilowii seed extract or Acanthopanax senticosus extract 1 was not significant. Acanthopanax senticosus extract 1 and Acanthopanax senticosus extract 2 had similar upregulation effects on ceramides, but recombinant humanized collagen did not have a synergistic effect with Acanthopanax senticosus extract 2 and Trichosanthes kirilowii seed extract.

[0070] Experimental Example 2: Evaluation of the efficacy of human skin barrier repair

[0071] The serums from Example 4 and Comparative Example 9 were tested for their skin repair efficacy on human skin. A total of 35 healthy Chinese men and women were tested, including 2 men and 33 women, aged 25 to 45 years, who met the criteria for voluntary inclusion and exclusion of subjects.

[0072] Directions for use: After cleansing and applying toner in the morning and evening, apply an appropriate amount of essence evenly to the face and gently massage until absorbed. Use twice daily, once in the morning and once in the evening, for 28 consecutive days.

[0073] Detection time: before using the sample (D0), 15 minutes after using the sample (15 min), 14 days after using the sample (D14), and 28 days after using the sample (D28).

[0074] Short-term test: Randomized half-face controlled trial. After cleansing their faces, subjects patted them dry with lint-free absorbent paper towels and sat quietly for at least 30 minutes until their skin condition stabilized. Then, testers applied appropriate amounts of the sample to the left cheek (Example 4) and the right cheek (Comparative Example 9). Before and 15 minutes after application, facial imaging tests, skin radiance tests, and skin moisture content tests were used to analyze the immediate differences between Example 4 and Comparative Example 9 (Tables 7-10). The results showed that Example 4 had a better immediate moisturizing and redness-reducing effect than Comparative Example 9, thus meeting consumers' immediate skincare needs.

[0075] Long-term testing: Subjects used the sample continuously for 28 days according to the instructions. Facial imaging, skin moisture content, and transepidermal water loss (TEWL) tests were performed before, 14 days after, and 28 days after use of sample example 4 to evaluate the sample's efficacy.

[0076] Test methods and test parameters:

[0077] 1. Facial Imaging Test (VISIA-CR)

[0078] The VISIA-CR facial image analyzer (Canfield, USA) provides safe and stable standard lighting conditions. All camera settings and imaging parameters are software-controlled to ensure image stability and standardization. The instrument features multiple light source modes: standard light, blue light, orange light, cross-polarized light, and parallel-polarized light, enabling multi-angle imaging of facial skin with image quality up to 21 megapixels. It also performs image analysis on red zone images to obtain a quantitative indicator, the red zone analysis a* value, used to assess the improvement in facial skin redness and sensitivity.

[0079] 2. Skin moisture content test (Corneometer)

[0080] The Corneometer (CM 825, Courage and Khazaka, Germany) is a skin moisture meter that tests skin moisture content based on the principle of capacitance. It is used to detect the relative water content on the skin surface; the higher the value, the higher the water content.

[0081] 3. Transdermal Water Loss (TEWL) Test (Aqua Flux)

[0082] This value is used to detect transepidermal water loss (TWEL) in the skin and assess skin barrier function. The lower the value, the better the water retention capacity.

[0083] The test results are shown in Tables 7-11.

[0084] 1. Red zone analysis a* value

[0085] The results of the red zone analysis a* value test before sample use (D0), 15 minutes after use (15 min), 14 days after use (D14), and 28 days after use (D28) are shown in the table below. The higher the red zone analysis a* value, the more severe the skin redness and the more severe the barrier damage.

[0086] Table 7. Descriptive statistics of a* values ​​in the red zone analysis (n=35)

[0087]

[0088] Table 8. Descriptive statistics of a* values ​​in red zone analysis (n=35)

[0089]

[0090] Statistical analysis results: "-": no statistically significant difference ( P ≥0.05); "*" "#": The difference is statistically significant (0.01≤ P < 0.05); "**" "##": The difference is statistically significant (0.001 ≤ P < 0.01); "***" "###": The difference is statistically significant ( P < 0.001).

[0091] 2. Skin moisture content test

[0092] The skin moisture content test results before sample use (D0), 15 minutes after use (15 min), 14 days after use (D14), and 28 days after use (D28) are shown in the table below. The higher the skin moisture content value, the higher the skin moisture content.

[0093] Table 9. Descriptive statistics of skin moisture content (n=35)

[0094]

[0095] Table 10. Statistical results of skin moisture content (n=35)

[0096]

[0097] Statistical analysis results: "-": no statistically significant difference ( P ≥ 0.05); "*" "#": The difference is statistically significant (0.01 ≤ P < 0.05); "**" "##": The difference is statistically significant (0.001 ≤ P < 0.01); "***" "###": The difference is statistically significant ( P < 0.001).

[0098] 3. Transdermal water loss (TEWL) test

[0099] The transdermal water loss (TEWL) values ​​were measured before use (D0), 14 days after use (D14), and 28 days after use (D28), as shown in the table below. A lower TEWL value indicates slower transdermal water loss and a more intact skin barrier.

[0100] Table 11 Descriptive statistics of transcutaneous water loss (TEWL) values ​​(n=35)

[0101]

[0102] Statistical analysis results: "-": no statistically significant difference ( P ≥ 0.05); "*": The difference is statistically significant (0.01 ≤ P < 0.05); "**": The difference is statistically significant (0.001 ≤ P < 0.01); "***": The difference is statistically significant ( P < 0.001).

[0103] As shown in Tables 7-11, during the 4-week testing period, the product containing the skin barrier repair composition (Example 4) significantly improved skin redness, reduced water loss, and increased skin hydration. Therefore, the essence product containing this skin barrier repair composition has good skin barrier repair and moisturizing effects.

[0104] Experimental Example 3:

[0105] The samples from Examples 1-3 and Comparative Examples 1-8 were placed at room temperature, -18℃, 4℃, and 45℃ respectively, and subjected to thermal cycling (-18℃, 4℃, 45℃, once every 3 days) for the corresponding time to observe stability. The stability of the product was judged by whether precipitation occurred. If no precipitation or instability occurred, the stability was considered passed. The specific results are shown in Table 12 below.

[0106] Table 12 Stability test results

[0107]

[0108] Note: √ indicates that the stability has passed and there is no precipitation; × indicates slight precipitation; ×× indicates severe precipitation; ○ indicates that the solution color has changed and the color has darkened slightly; ○○ indicates that the solution color has changed and the color has darkened significantly.

[0109] Table 12 shows that Trichosanthes kirilowii seed extract alone exhibits color darkening under room temperature, high temperature, and thermal cycling conditions. Acanthopanax senticosus extract 1 alone shows precipitation under low temperature and thermal cycling conditions. The stability issues cannot be effectively resolved when either is combined with recombinant humanized collagen. A mixture of Trichosanthes kirilowii seed extract and Acanthopanax senticosus extract also fails the stability test. However, when recombinant humanized collagen, Trichosanthes kirilowii seed extract, and Acanthopanax senticosus extract are present together, the stability test is passed. Acanthopanax senticosus extract 2 alone also shows precipitation under low temperature and thermal cycling conditions, but the precipitation is more pronounced than that of Acanthopanax senticosus extract 1. When recombinant humanized collagen, Trichosanthes kirilowii seed extract, and Acanthopanax senticosus extract 2 are present together, the stability test is also failed.

[0110] The above embodiments are only for illustrating the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand the content of the present invention and implement it accordingly. They should not be construed as limiting the scope of protection of the present invention. All equivalent changes or modifications made in accordance with the spirit and essence of the present invention should be covered within the scope of protection of the present invention.

Claims

1. A composition having a skin barrier repair effect, characterized by, The ingredients, by weight, include: 0.01-1.0 parts of recombinant humanized collagen, 1.0-15.0 parts of Trichosanthes kirilowii extract, and 1.0-15.0 parts of Acanthopanax senticosus extract.

2. The composition of claim 1, wherein The ingredients, by weight, include: 0.1-0.5 parts recombinant humanized collagen, 5.0-10.0 parts Trichosanthes kirilowii extract, and 5.0-10.0 parts Acanthopanax senticosus extract.

3. The composition according to claim 1, characterized in that, The recombinant humanized collagen has a molecular weight of 70,000 Da.

4. The composition according to claim 1, characterized in that, The extract of the gourd seed is obtained by alcohol extraction and concentration of dried gourd seed powder.

5. The composition according to claim 4, characterized in that, The preparation method of the melon seed extract is as follows: take dried melon seed powder, add 60%~80% ethanol solution, extract by water bath heating at 60~80℃ 2~4 times, combine the filtrates, concentrate under reduced pressure, volatilize until there is no alcohol odor, add 0.8~1.2 times the mass of butanediol, stir evenly, filter, and the extract is obtained.

6. The composition according to claim 1, characterized in that, The Acanthopanax senticosus extract was prepared using pulsed ultrasound-assisted subcritical extraction.

7. The composition according to claim 6, characterized in that, The preparation method of the Acanthopanax senticosus extract is as follows: (1) The dried Acanthopanax senticosus was pulverized and sieved, and water was added for pulsed ultrasonic extraction at room temperature. The filtrate was then filtered. (2) Add water to the filtrate in step (1) and carry out subcritical extraction at a temperature of 120-140℃ and a pressure of 0.8-1.2MPa. Concentrate the filtrate under reduced pressure to 4-6 times the mass of Acanthopanax senticosus powder, add 1-1.5 times the amount of glycerol to the concentrate, stir and mix evenly, and then filter to obtain the final product.

8. The use of the composition according to any one of claims 1-7 in the preparation of a product having a skin barrier repairing effect, further wherein the product is a cosmetic.

9. A cosmetic product, characterized in that, The cosmetic product comprises at least the composition according to any one of claims 1-7, and further, the cosmetic product is a skin care product.

10. The cosmetic as claimed in claim 9, characterized in that the amount of the composition added is 1-35% based on the total mass of the cosmetic.

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