Device for directional screening of stress-resistant strains and screening method using the same

Abstract

The application discloses a kind of anti-reverse strain directional screening device and its screening use method, it is related to microorganism screening technical field, device includes the shell with first cavity and second cavity, oxygen module, air pressure module, temperature regulating module and controller are established in first cavity, second cavity is sequentially separated into sample room, processing room and buffer room with conveying unit, each conveying surface is linked to each other to transport petri dish, wherein the internal cavity of processing room can be independently sealed and is equipped with intensity adjustable ultraviolet lamp, controller is respectively communicated with oxygen module, intensity adjustable ultraviolet lamp, air pressure module, temperature regulating module and each conveying unit communication connection, to realize the synergic control of oxygen inlet, pressure regulation, temperature regulation and ultraviolet irradiation. Its method includes sterilization treatment, setting environmental parameter, and supplement new to be handled petri dish to cycle operation. It can realize multi-parameter synchronous screening, realize the efficient and accurate directional screening of anti-reverse strain.

Description

Technical Field

[0001] This invention relates to the field of microbial screening technology, and in particular to a targeted screening device for stress-resistant strains and its screening method. Background Technology

[0002] Traditional fermented foods from the plateau region are an important part of the regional food culture and health industry, as well as a vital pillar of the regional economy. Their unique flavor and nutritional value have made them an important geographical indication of the plateau area. However, due to the extreme environment of the plateau (low temperature, low oxygen, low pressure, etc.) and the limitations of natural fermentation methods, the plateau fermented food industry has long faced key technical bottlenecks such as low activity of traditional microbial communities, long fermentation cycles, and unstable quality. These bottlenecks severely restrict the large-scale production and market promotion of the products. Therefore, there is an urgent need to screen new stress-resistant strains to enrich the plateau fermentation microbial strain bank.

[0003] Existing strain screening equipment can only screen under single environmental conditions, making it difficult to fully reflect the actual complex and extreme environmental conditions. The screened strains are not suitable for actual application scenarios. The environmental conditions in plateau areas are complex and extreme, combining harsh conditions such as low temperature, low oxygen, low pressure, and strong ultraviolet radiation. Currently, there is no dedicated equipment that can simultaneously screen plateau stress-resistant strains for multiple environmental parameters, which has become a major technical obstacle restricting the development of the plateau fermentation industry. Summary of the Invention

[0004] The purpose of this invention is to provide a targeted screening device for stress-resistant strains and its screening method to solve the problems existing in the prior art. It can realize simultaneous screening of multiple parameters and achieve efficient and accurate targeted screening of stress-resistant strains.

[0005] To achieve the above objectives, the present invention provides the following solution: This invention provides a targeted screening device for stress-resistant strains, comprising: a shell having a first cavity and a second cavity; the first cavity containing an oxygen module, a pressure module, and a temperature control module; the second cavity being sequentially divided into a sample chamber, a processing chamber, and a buffer chamber, each of which has a conveying unit, the conveying surfaces of which are interconnected, and the conveying surfaces of the conveying units in the sample chamber being used to place culture dishes coated with target microorganisms; the internal cavity of the processing chamber being independently sealable; the oxygen module for introducing oxygen into the processing chamber, the pressure module for adjusting the internal pressure of the processing chamber, and the temperature control module for adjusting the internal temperature of the processing chamber; and at least one adjustable-intensity ultraviolet lamp in the processing chamber; and a controller disposed in the first cavity, which is communicatively connected to the oxygen module, the adjustable-intensity ultraviolet lamp, the pressure module, the temperature control module, and each of the conveying units.

[0006] Preferably, the temperature control module includes a liquid nitrogen storage tank, a cooling pipe, a first pump body, and a gas-liquid separator; the liquid nitrogen storage tank is used to connect to an external liquid nitrogen supply device, and the liquid nitrogen storage tank contains liquid nitrogen medium; the cooling pipe is disposed in the processing chamber; the inlet of the first pump body is connected to the outlet of the liquid nitrogen storage tank; the outlet of the first pump body is connected to one end of the cooling pipe; the other end of the cooling pipe is connected to the inlet of the gas-liquid separator; the liquid outlet of the gas-liquid separator is connected to the liquid nitrogen storage tank; a temperature sensor is placed in the processing chamber; the controller is communicatively connected to both the first pump body and the temperature sensor.

[0007] Preferably, the pressure module includes a nitrogen storage tank, which is connected to the gas outlet of the gas-liquid separator; a pressure sensor is installed in the processing chamber; and the controller is communicatively connected to the pressure sensor.

[0008] Preferably, the sample chamber, the processing chamber, and the buffer chamber are all equipped with opening and closing doors; a connecting port is provided between the sample chamber and the processing chamber, and between the processing chamber and the buffer chamber, and an automatic lifting and sealing door is provided at each connecting port; the controller can control the opening and closing of each of the automatic lifting and sealing doors at the connecting port.

[0009] Preferably, the conveying unit has a rotating conveyor belt, and the cooling pipe is disposed below the conveying surface of the conveyor belt.

[0010] Preferably, the sample chamber is equipped with a sterilization ultraviolet lamp.

[0011] Preferably, the processing chamber is equipped with an oxygen concentration sensor that is communicatively connected to the controller.

[0012] Preferably, an L-shaped partition is fixedly provided inside the housing to separate the inner cavity of the housing to form the first cavity and the second cavity; the upper end of the L-shaped partition is fixedly connected to the inner top of the housing and has a gap between it and the inner sidewall of the rear plate of the housing; the other end of the L-shaped partition is fixedly connected to the inner sidewall of the front plate of the housing and has a gap between it and the inner bottom of the housing; the second cavity is separated by two partitions to form the sample chamber, the processing chamber and the buffer chamber respectively; an inclined plate is provided on the front plate of the housing, and the opening and closing doors of the sample chamber and the processing chamber are respectively provided on the inclined plate.

[0013] Preferably, a display screen is fixedly mounted on the inclined plate, and the display screen is communicatively connected to the controller.

[0014] The present invention also provides a screening method based on the stress-resistant strain directional screening device as described in any of the preceding claims, comprising the following steps: S1, the sample chamber, the processing chamber and the buffer chamber are all sterilized; S2, the controller sets various environmental parameters required for sample processing, including temperature, oxygen concentration, air pressure and ultraviolet intensity; S3, place each culture dish coated with the target microorganism on the conveying surface of the conveying unit in the sample chamber; start the controller so that each culture dish passes through the sample chamber and the processing chamber in sequence and then enters the buffer chamber to complete a single processing; S4, remove the processed petri dish and place the new petri dish to be processed on the conveying surface of the conveying unit in the sample chamber.

[0015] The present invention achieves the following technical effects compared to the prior art: The stress-resistant strain targeted screening device provided by this invention constructs a streamlined layout of a sample chamber, a processing chamber, and a buffer chamber through a second cavity within the shell, and realizes automated sample transfer using a connectable transfer unit, thereby replacing traditional manual operation and effectively shortening the processing cycle. More importantly, the device creatively integrates an oxygen module, a pressure module, a temperature control module, and an adjustable intensity ultraviolet lamp within the independent sealed space of the processing chamber. This allows the device to simultaneously simulate various extreme conditions in the plateau environment, such as low oxygen, low pressure, low temperature, and strong ultraviolet irradiation. The controller acts as the central system to coordinate and communicate with these modules, ensuring precise control of environmental parameters and stable application of combined stresses. This solves the problem in existing technologies where single-environment screening makes it difficult for strains to adapt to actual application scenarios, thus achieving targeted and efficient screening of plateau stress-resistant strains.

[0016] This invention also provides a screening method for a stress-resistant strain targeted screening device. Through unified sterilization in S1, the sample chamber, processing chamber, and buffer chamber are ensured to be sterile, eliminating interference from microbial contamination on the screening results. Parameter presets in S2, combined with automated transfer in S3, allow the culture dishes to be processed strictly according to set parameters such as temperature, oxygen concentration, air pressure, and ultraviolet intensity under the unified scheduling of the controller, ensuring the consistency and repeatability of experimental conditions. The cyclical operation step in S4, which involves promptly replenishing new samples after the processed culture dishes are removed, breaks the intermittent limitations of traditional batch processing. Utilizing the connecting characteristics of the transfer unit, a continuous workflow is formed, significantly improving the utilization efficiency of the screening device and the sample processing volume per unit time. This effectively solves the technical problems of low screening efficiency and long cycles caused by incomplete environmental simulation and operational interruptions during the screening of stress-resistant strains in high-altitude areas. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 A schematic diagram of the overall structure of the stress-resistant strain targeted screening device provided by the present invention; Figure 2 This is a schematic diagram of the overall structure of the stress-resistant strain targeted screening device provided by the present invention from another perspective. Figure 3 This is a schematic diagram of the structure of the stress-resistant strain targeted screening device provided by the present invention after removing some of the opening and closing doors and the display screen; Figure 4 A schematic diagram of the internal structure of the stress-resistant strain targeted screening device provided by the present invention from a first perspective. Figure 5 A schematic diagram of the internal structure of the stress-resistant strain targeted screening device provided by the present invention from a second perspective. Figure 6 This is a schematic diagram of the internal structure of the stress-resistant strain targeted screening device provided by the present invention from a third-person perspective.

[0019] In the picture: 1-Shell; 11-Sample chamber; 12-Processing chamber; 13-Buffer chamber; 14-Opening and closing door; 15-L-shaped partition; 16-Separator plate; 17-Automatic lifting sealing door; 18-Display screen; 19-One-way vent valve; 101-Front plate; 102-Inclined plate; 103-Bottom plate; 104-Heat dissipation hole; 2-Liquid nitrogen storage tank; 21-Cooling pipe; 22-Gas-liquid separator; 23-Nitrogen storage tank; 3-Transmission unit; 4- Sterilization UV lamp; 41- Adjustable intensity UV lamp; 5 - Oxygen cylinder; 51 - Oxygen concentration sensor; 6- Petri dish; 7-Controller. Detailed Implementation

[0020] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] The purpose of this invention is to provide a targeted screening device for stress-resistant strains and its screening method to solve the problems existing in the prior art. It can realize simultaneous screening of multiple parameters and achieve efficient and accurate targeted screening of stress-resistant strains.

[0022] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0023] Example 1 This embodiment provides a device for targeted screening of stress-resistant strains, such as... Figures 1-6 As shown, it includes: The housing 1 has a first cavity and a second cavity; the first cavity is equipped with an oxygen module, a pressure module, and a temperature control module; the second cavity is sequentially divided into a sample chamber 11, a processing chamber 12, and a buffer chamber 13, and each of the sample chamber 11, processing chamber 12, and buffer chamber 13 is equipped with a conveying unit 3, the conveying surfaces of each conveying unit 3 can be connected to each other, and the conveying surface of the conveying unit 3 in the sample chamber 11 is used to place each culture dish 6 coated with the target microorganism; the internal cavity of the processing chamber 12 can be independently sealed; the oxygen module is used to introduce oxygen into the processing chamber 12, the pressure module can regulate the internal pressure of the processing chamber 12, the temperature control module is used to regulate the internal temperature of the processing chamber 12, and at least one adjustable intensity ultraviolet lamp 41 is provided in the processing chamber 12. The controller 7 is located in the first cavity and is communicatively connected to the oxygen module, the adjustable intensity ultraviolet lamp 41, the air pressure module, the temperature control module, and each transmission unit 3.

[0024] The streamlined layout of the sample chamber 11, processing chamber 12, and buffer chamber 13 is constructed through the second cavity within the shell 1, and the automated transfer of samples is achieved using the connectable transfer unit 3, thereby replacing traditional manual operation and effectively shortening the processing cycle. More importantly, the device creatively integrates an oxygen module, a pressure module, a temperature control module, and an adjustable intensity ultraviolet lamp 41 within the independent sealed space of the processing chamber 12. This allows the device to simultaneously simulate various extreme conditions in the plateau environment, such as low oxygen, low pressure, low temperature, and strong ultraviolet radiation. The controller 7 acts as the central system to coordinate and communicate with these modules, ensuring precise control of environmental parameters and stable application of combined stresses. This solves the problem in existing technologies where single-environment screening makes it difficult for strains to adapt to actual application scenarios, and enables targeted and efficient screening of plateau-resistant strains.

[0025] The following are the relevant settings for housing 1: Among the optional solutions in this embodiment, the more preferred one is as follows: Figures 1-3As shown, the sample chamber 11, the processing chamber 12 and the buffer chamber 13 are all equipped with opening and closing doors 14; there are connecting ports between the sample chamber 11 and the processing chamber 12 and between the processing chamber 12 and the buffer chamber 13, and automatic lifting sealing doors 17 are provided at the connecting ports; the controller 7 can control the opening and closing of each automatic lifting sealing door 17 at the connecting ports.

[0026] Specifically, the buffer chamber 13 has a display screen 18 positioned on the inclined plate 102 for easy operation. A corresponding opening and closing door 14, a semi-transparent shielded door with a quick-release mechanism, is located at the end of the housing 1. The processed petri dishes 6 can be directly viewed by eye; alternatively, alarm devices can be strategically installed as needed. These devices, in conjunction with corresponding visual sensors, will alert the operator when a processed petri dish 6 is present in the buffer chamber 13.

[0027] Among the optional solutions in this embodiment, the more preferred one is as follows: Figures 4-6 As shown, an L-shaped partition 15 is fixedly provided inside the housing 1, which is used to separate the inner cavity of the housing 1 to form a first cavity and a second cavity; the upper end of the L-shaped partition 15 is fixedly connected to the inner top of the housing 1, and there is a gap between it and the inner side wall of the rear plate of the housing 1; the other end of the L-shaped partition 15 is fixedly connected to the inner side wall of the front plate 101 of the housing 1, and there is a gap between it and the inner bottom of the housing 1; the second cavity is separated by two partitions 16 to form a sample chamber 11, a processing chamber 12 and a buffer chamber 13 respectively; an inclined plate 102 is provided on the front plate 101 of the housing 1, and the opening and closing doors 14 of the sample chamber 11 and the processing chamber 12 are respectively provided on the inclined plate 102.

[0028] Specifically, the exterior of the housing 1 is composed of a top plate, a bottom plate 103, a front plate 101, a rear plate, and two side plates (the opening and closing door 14 of the buffer chamber 13 is set on the corresponding side plate). The inclined plate 102 is set on the front plate 101. The inclined plate 102 is set to provide better visual observation and operation.

[0029] Specifically, the sample to be induced is first placed on the conveyor belt (i.e., conveyor unit 3) of the sample chamber 11, and after being sterilized by the sterilization ultraviolet lamp 4, it is sent to the processing chamber 12 by the conveyor belt.

[0030] Specifically, regarding the processing chamber 12, there are three ultraviolet lamps with adjustable irradiance (i.e., adjustable intensity ultraviolet lamps 41) above the conveyor belt inside the processing chamber 12 to induce mutations in the sample. In the middle of the conveyor belt is a U-shaped cooling pipe 21 to cool the sample. On both sides of the processing chamber 12 are two automatically lifting high-pressure sealing plates (i.e., automatic lifting sealing doors 17, which are existing technologies that can be controlled by the controller 7 to achieve sealing, and will not be described in detail here). After the sample is sent into the processing chamber 12 via the conveyor belt, the high-pressure sealing plates automatically descend to the designated position to seal the corresponding positions on both sides of the processing chamber 12 (i.e., the connecting port seal). When there is insufficient oxygen in the processing chamber 12, the oxygen inlet opens, and oxygen from the oxygen tank 5 is introduced into the processing chamber 12.

[0031] Among the optional solutions in this embodiment, the more preferred one is as follows: Figure 3 and Figure 4 As shown, the conveying unit 3 has a rotating conveyor belt, and the cooling pipe 21 is located below the conveying surface of the conveyor belt (there is a gap between the cooling pipe 21 and the conveying surface of the conveyor belt; the liquid nitrogen medium in the cooling pipe 21 does not directly contact the sample, and the liquid nitrogen medium only serves as an indirect cold source).

[0032] Among the optional solutions in this embodiment, the more preferred one is as follows: Figure 4 As shown, the sample chamber 11 is equipped with a sterilization ultraviolet lamp 4.

[0033] Among the optional solutions in this embodiment, the more preferred one is as follows: Figure 1 As shown, a display screen 18 is fixedly mounted on the inclined plate 102, and the display screen 18 is communicatively connected to the controller 7.

[0034] The following are the settings for temperature control within processing chamber 12: Among the optional solutions in this embodiment, the more preferred one is as follows: Figures 1-6 As shown, the temperature control module includes a liquid nitrogen storage tank 2, a cooling pipe 21, a first pump body, and a gas-liquid separator 22. The liquid nitrogen storage tank 2 is used to connect to an external liquid nitrogen supply device (i.e., the entire device needs to be connected to an external liquid nitrogen supply device, such as a liquid nitrogen generator), and the liquid nitrogen storage tank 2 contains liquid nitrogen. The cooling pipe 21 is located in the processing chamber 12. The inlet of the first pump body is connected to the outlet of the liquid nitrogen storage tank 2, and the outlet of the first pump body is connected to one end of the cooling pipe 21. The other end of the cooling pipe 21 is connected to the inlet of the gas-liquid separator 22, and the liquid outlet of the gas-liquid separator 22 is connected to the liquid nitrogen storage tank 2. A temperature sensor is built into the processing chamber 12. The controller 7 is communicatively connected to both the first pump body and the temperature sensor.

[0035] Specifically, in addition to the aforementioned devices for lowering the temperature of the processing chamber 12, the temperature control module can also include a heating compensation unit. This unit compensates for the temperature drop in the processing chamber 12 when it falls below the target setpoint. The controller 7 coordinates the control of the cryogenic cooling unit (including components such as the liquid nitrogen storage tank 2, cooling pipe 21, first pump, and gas-liquid separator 22) and the heating compensation unit based on feedback signals from the temperature sensor, maintaining the temperature of the processing chamber 12 within the preset range. The purpose of the heating compensation unit is to improve the temperature closed-loop control structure, prevent temperature overshoot during cryogenic control, and enhance temperature stability and device feasibility.

[0036] Specifically, liquid nitrogen is used as a medium to enter the cooling pipe 21 located in the processing chamber 12 through the first pump body. The cooling pipe 21 indirectly transfers heat to the culture dish 6 on the conveyor belt through the space of the processing chamber 12. Then, the unvaporized liquid nitrogen is recovered and the vaporized nitrogen is collected through the gas-liquid separator 22 (by controlling the liquid nitrogen evaporation rate, a stable ultra-low temperature gas flow is obtained to cool external samples and systems or to precisely control the temperature).

[0037] Specifically, through indirect heat exchange and temperature feedback control via cooling pipe 21, the sample is kept within a set low-temperature stress range (i.e., the temperature conditions within the controlled treatment chamber 12) to distinguish the tolerance, survival ability, and recovery growth ability of different strains under low temperature or combined stress.

[0038] Specifically, the L-shaped partition 15, the partition plate 16, and the automatic lifting sealing door 17 are equipped with insulation layers. At the same time, the opening and closing door 14 of the processing chamber 12 uses double-layer insulated glass, thereby achieving a wider range of temperature conditions.

[0039] Specifically, the existing equipment has a narrow range of adjustable environmental condition parameters (such as the temperature cannot be adjusted to below zero), which is only suitable for screening conventional environmental conditions and cannot be applied to some special scenarios. Therefore, a temperature control module is set up to achieve a wider range of temperature conditions.

[0040] Specifically, the prepared liquid nitrogen is temporarily stored in liquid nitrogen storage tank 2. When cooling is required, the liquid nitrogen medium in liquid nitrogen storage tank 2 is pumped into cooling pipe 21 (composed of multiple sections, with two connected sections forming a U-shape) through the first pump body. The sample is indirectly cooled by heat exchange through the U-shaped loop, and the temperature of processing chamber 12 is regulated in a closed loop by temperature sensor and controller 7. The liquid nitrogen enters gas-liquid separator 22 from the liquid nitrogen outlet. The sublimated nitrogen gas will be suspended at the top of gas-liquid separator 22 and collected into nitrogen storage tank 23 to pressurize processing chamber 12. The unsublimated liquid nitrogen remains at the bottom of gas-liquid separator 22 and is collected back into liquid nitrogen storage tank 2.

[0041] Specifically, The following are the settings for pressure control within processing chamber 12: Among the optional solutions in this embodiment, the more preferred one is as follows: Figures 3-6 As shown, the pressure module includes a nitrogen storage tank 23, which is connected to the gas outlet of the gas-liquid separator 22; the processing chamber 12 has a built-in pressure sensor; and the controller 7 is communicatively connected to the pressure sensor.

[0042] The following are the settings for controlling the oxygen concentration in processing chamber 12: Among the optional solutions in this embodiment, the more preferred one is as follows: Figure 4 As shown, the processing chamber 12 is equipped with an oxygen concentration sensor 51 that is connected to the controller 7 in communication.

[0043] Specifically, the oxygen module includes an oxygen tank 5 and a second pump body. The outlet of the oxygen tank 5 is connected to the inlet of the second pump body, and the outlet of the second pump body is connected to the interior of the processing chamber 12.

[0044] Specifically, throttle valves, two-way valves, and other components can be reasonably installed at the outlet of oxygen tank 5 as needed to better regulate the supply flow.

[0045] The following are the settings for controlling the ultraviolet intensity in treatment chamber 12: Specifically, the treatment chamber 12 is equipped with three adjustable intensity ultraviolet lamps 41, whose placement and quantity can be reasonably set according to actual needs.

[0046] Regarding other relevant explanations: Specifically, the key to screening stress-resistant strains is not to allow microorganisms to continue metabolic growth at extremely low temperatures, but rather to apply standardized environmental stresses to different strains and then assess their tolerance differences through recovery culture and phenotypic evaluation after treatment. For example, in screening strains for fermented foods in high-altitude areas, the temperature of the treatment chamber can be set to a range close to or even more stringent than the low-temperature environment of high altitudes, combined with hypoxia, low pressure, and ultraviolet treatment, to screen candidate strains that still exhibit good survival, recovery growth, or fermentation performance after stress. This screening logic differs from liquid nitrogen cryopreservation and belongs to a targeted screening process of "low temperature / complex environmental stress—recovery culture—performance evaluation".

[0047] Specifically, the effects achievable by using the stress-resistant strain targeted screening device provided in this embodiment are further explained below: 1. Synchronous parameter control and high degree of automation: The four core parameters of temperature, ultraviolet intensity, oxygen concentration and air pressure can be set synchronously through the environmental condition setting interface. After the device is started, it will automatically complete the precise control of each condition without manual intervention.

[0048] 2. Liquid nitrogen recycling and reuse, streamlined and efficient structure: Low temperature control is achieved by using liquid nitrogen sublimation, which can lower the temperature of the treatment chamber 12 to as low as -20℃ (a temperature sensor is installed in the treatment chamber 12, and the controller 7 is connected to the temperature sensor and the first pump body. It can adjust the flow rate, on / off time, or pump working status of the liquid nitrogen medium entering the cooling pipe 21 according to the feedback signal of the temperature sensor, so as to control the temperature of the treatment chamber 12 within the preset screening range; the minimum temperature of the treatment chamber is preferably -20℃, and the specific screening temperature is set according to the strain type and screening purpose). The nitrogen gas generated by sublimation can be directly used for gas pressure and oxygen concentration regulation. A single circulation system realizes multi-parameter control, which not only improves work efficiency, but also greatly simplifies the device structure and reduces equipment costs.

[0049] 3. Fully automatic continuous operation with excellent sterility: The fully automatic sample feeding and discharging is achieved by relying on the conveyor belt. The loading and unloading operations can be completed without opening the processing chamber 12. Multiple samples can be processed continuously. With the pretreatment UV sterilization and the sealed design of the processing process, the sterile environment of the processing chamber 12 can be maintained at all times, avoiding the influence of other bacteria on the screening results.

[0050] 4. Easy to operate and highly applicable: The device has a simple overall structure, and all operation settings can be completed through the display screen 18, making it easy to learn; multiple environmental parameters can be flexibly adjusted to meet the targeted screening needs of different stress-resistant strains.

[0051] Example 2 This embodiment provides a screening method based on the stress-resistant strain targeted screening device of Embodiment 1, including the following steps: S1, sterilize the sample chamber 11, processing chamber 12 and buffer chamber 13; S2, through controller 7, sets various environmental parameters required for sample processing, including temperature, oxygen concentration, air pressure and ultraviolet intensity; S3, place each culture dish 6 coated with the target microorganism on the conveying surface of the conveying unit 3 in the sample chamber 11; start the controller 7 so that each culture dish 6 passes through the sample chamber 11 and the processing chamber 12 in sequence and then enters the buffer chamber 13 to complete a single processing. S4, the processed petri dish 6 is removed, and a new petri dish 6 to be processed is placed on the conveying surface of the conveying unit 3 in the sample chamber 11.

[0052] The unified sterilization process in S1 ensures that the sample chamber 11, processing chamber 12, and buffer chamber 13 are in a sterile state, eliminating the interference of microbial contamination on the screening results. The parameter preset in S2, combined with the automated transfer in S3, allows the petri dishes 6 to be processed strictly according to the set multi-dimensional parameters such as temperature, oxygen concentration, air pressure, and ultraviolet intensity under the unified scheduling of the controller 7, ensuring the consistency and repeatability of experimental conditions. The cyclic operation step in S4, which replenishes new samples to be processed in a timely manner after the processed petri dishes 6 are removed, breaks the intermittent limitation of traditional batch processing. It utilizes the connecting characteristics of the transfer unit 3 to form a continuous workflow, thereby significantly improving the utilization efficiency of the screening device and the sample processing volume per unit time. This effectively solves the technical problems of low screening efficiency and long cycle caused by incomplete environmental simulation and operation interruption in the screening of plateau stress-resistant strains.

[0053] Specifically, regarding S1: Pretreatment sterilization: Turn on all the UV lamps in sample chamber 11 and processing chamber 12 in advance (the sterilization equipment in buffer chamber 13 can be reasonably set according to actual needs, such as the same UV lamps) to perform comprehensive sterilization on both chambers and ensure a sterile environment for subsequent screening processes.

[0054] Specifically, regarding S2: Environmental parameter setting and control: After starting the device, set the various environmental parameters required for sample processing via display screen 18, including temperature, oxygen concentration, air pressure, and ultraviolet intensity. The specific control mechanism is as follows: Temperature control: This is achieved through liquid nitrogen sublimation. The liquid nitrogen medium in the liquid nitrogen storage tank 2 is pumped to the cooling pipe 21 via the first pump, and the U-shaped cooling pipe 21 directly cools the treatment chamber 12 to the target temperature. The nitrogen gas produced by sublimation rises to the upper part of the gas-liquid separator 22 and is collected in the nitrogen storage tank 23 for recycling; the unsublimated liquid nitrogen remains at the bottom of the gas-liquid separator 22 and is also recycled to the liquid nitrogen storage tank 2, forming a closed loop (the low-temperature treatment is a stress screening step; after treatment, the culture dish 6 can be transferred to a suitable temperature condition for recovery culture, and the stress resistance can be evaluated based on the colony formation ability, growth rate, or fermentation performance).

[0055] Pressure regulation: This is achieved by introducing or releasing nitrogen gas. The nitrogen gas used for regulation is derived from the sublimation products of liquid nitrogen, and no additional gas source is required.

[0056] Oxygen concentration control: When the concentration is lower than the set value, the oxygen in the oxygen tank 5 is directly delivered to the treatment chamber 12 via the second pump body; when the concentration is higher than the set value, the one-way vent valve 19 is opened (the bottom of the treatment chamber 12 is provided with an exhaust port, the exhaust port is provided with a one-way vent valve 19, and the bottom plate 103 of the shell 1 is provided with a heat dissipation hole 104, which is connected to the first cavity, and the four corners of the outer part of the shell 1 are respectively fixed with support legs) and nitrogen is pumped into the treatment chamber 12 to replace and discharge some air to reduce the oxygen concentration (the position of nitrogen entering the treatment chamber 12 can be reasonably set according to the effect to meet the purpose of replacing and discharging some air to reduce the oxygen concentration).

[0057] Ultraviolet intensity control: The intensity can be directly set by the adjustable ultraviolet lamp equipped with the device to meet different mutagenesis requirements.

[0058] Specifically, regarding S3: The culture dishes 6 coated with the target microorganisms are placed sequentially on the feed conveyor belt (i.e., the conveyor belt of the sample chamber 11). After starting the drive motor (each conveyor belt is driven to rotate by its corresponding drive motor to achieve the conveying function), the conveyor belt moves the culture dishes 6 towards the processing chamber 12. At this time, the automatic lifting sealing door 17 on the left side of the processing chamber 12 (i.e., the sealing door at the connection between the sample chamber 11 and the processing chamber 12) automatically opens. After the culture dishes 6 have completely entered the processing chamber 12, the automatic lifting sealing door 17 on the left side immediately closes, and the device automatically starts the mutagenesis treatment program (i.e., the controller 7 controls each component to provide the set environmental conditions).

[0059] Specifically, regarding S4: Sample discharge and continuous operation: After the mutagenesis treatment is completed, the automatic lifting and sealing door 17 on the right side of the treatment chamber 12 automatically opens, and the petri dish 6 is moved out of the treatment chamber 12 by the conveyor belt, completing a single treatment. At this time, a new petri dish 6 to be treated can be placed directly on the feed end (i.e., on the conveyor belt in the sample chamber 11), and the device automatically enters the next sample processing flow, realizing continuous operation.

[0060] Specific examples have been used to illustrate the principles and implementation methods of this invention. The descriptions of the above embodiments are only for the purpose of helping to understand the method and core ideas of this invention. Furthermore, those skilled in the art will recognize that, based on the ideas of this invention, there will be changes in the specific implementation methods and application scope. Therefore, the content of this specification should not be construed as a limitation of this invention.

Claims

1. A device for targeted screening of stress-resistant strains, characterized in that: include: The housing has a first cavity and a second cavity; the first cavity contains an oxygen module, a pressure module, and a temperature control module; the second cavity is sequentially divided into a sample chamber, a processing chamber, and a buffer chamber, each of which is equipped with a transfer unit, the transfer surfaces of which are interconnected; the transfer surface of the transfer unit in the sample chamber is used to place petri dishes coated with target microorganisms; the internal cavity of the processing chamber can be independently sealed; the oxygen module is used to introduce oxygen into the processing chamber, the pressure module can regulate the internal pressure of the processing chamber, the temperature control module is used to regulate the internal temperature of the processing chamber, and at least one adjustable ultraviolet lamp is provided in the processing chamber. The controller is located in the first cavity and is communicatively connected to the oxygen module, the adjustable ultraviolet lamp, the air pressure module, the temperature control module, and each of the transmission units.

2. The targeted screening device for stress-resistant strains according to claim 1, characterized in that: The temperature control module includes a liquid nitrogen storage tank, a cooling pipe, a first pump body, and a gas-liquid separator. The liquid nitrogen storage tank is used to connect to an external liquid nitrogen supply device, and the liquid nitrogen storage tank contains liquid nitrogen medium. The cooling pipe is located in the processing chamber. The inlet of the first pump body is connected to the outlet of the liquid nitrogen storage tank. The outlet of the first pump body is connected to one end of the cooling pipe. The other end of the cooling pipe is connected to the inlet of the gas-liquid separator. The liquid outlet of the gas-liquid separator is connected to the liquid nitrogen storage tank. A temperature sensor is installed in the processing chamber; The controller is communicatively connected to both the first pump body and the temperature sensor.

3. The targeted screening device for stress-resistant strains according to claim 2, characterized in that: The pressure module includes a nitrogen storage tank, which is connected to the gas outlet of the gas-liquid separator; A pressure sensor is installed in the processing chamber; The controller is communicatively connected to the pressure sensor.

4. The targeted screening device for stress-resistant strains according to claim 1, characterized in that: The sample chamber, the processing chamber, and the buffer chamber are all equipped with openable and closed doors; A connecting opening is provided between the sample chamber and the processing chamber, and between the processing chamber and the buffer chamber, and an automatic lifting and sealing door is provided at the connecting opening; The controller can control the opening and closing of the communication port by each of the automatic lifting and sealing doors.

5. The targeted screening device for stress-resistant strains according to claim 2, characterized in that: The conveying unit has a rotating conveyor belt, and the cooling pipe is disposed below the conveying surface of the conveyor belt.

6. The targeted screening device for stress-resistant strains according to claim 1, characterized in that: The sample chamber is equipped with a sterilization ultraviolet lamp.

7. The targeted screening device for stress-resistant strains according to claim 1, characterized in that: The processing chamber is equipped with an oxygen concentration sensor that is communicatively connected to the controller.

8. The targeted screening device for stress-resistant strains according to claim 4, characterized in that: An L-shaped partition is fixedly provided inside the housing to separate the inner cavity of the housing to form the first cavity and the second cavity; the upper end of the L-shaped partition is fixedly connected to the inner top of the housing and has a gap between it and the inner sidewall of the rear plate of the housing; the other end of the L-shaped partition is fixedly connected to the inner sidewall of the front plate of the housing and has a gap between it and the inner bottom of the housing. The second cavity is divided by two partition plates to form the sample chamber, the processing chamber and the buffer chamber respectively; the front plate of the housing is provided with an inclined plate, and the opening and closing doors of the sample chamber and the processing chamber are respectively provided on the inclined plate.

9. The targeted screening device for stress-resistant strains according to claim 8, characterized in that: A display screen is fixedly mounted on the inclined plate, and the display screen is communicatively connected to the controller.

10. A method for screening using the stress-resistant strain directional screening device based on any one of claims 1 to 9, characterized in that: Includes the following steps: S1, the sample chamber, the processing chamber and the buffer chamber are all sterilized; S2, the controller sets various environmental parameters required for sample processing, including temperature, oxygen concentration, air pressure and ultraviolet intensity; S3, place each culture dish coated with the target microorganism on the conveying surface of the conveying unit in the sample chamber; start the controller so that each culture dish passes through the sample chamber and the processing chamber in sequence and then enters the buffer chamber to complete a single processing; S4, remove the processed petri dish and place the new petri dish to be processed on the conveying surface of the conveying unit in the sample chamber.

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