Multifunctional synergistic mosquito-repelling ecological seed package and application thereof

Abstract

The application provides a multifunctional synergistic mosquito-repelling ecological seed package and application thereof, and belongs to the technical field of pest control, and particularly relates to a multifunctional synergistic mosquito-repelling ecological seed package, which is a seed composition, and contains repelling seeds, aromatic auxiliary seeds and long-acting protective seeds, wherein the repelling seeds, the aromatic auxiliary seeds and the long-acting protective seeds are in a mass ratio of 3-5:1-2:2-4; the multifunctional synergistic mosquito-repelling ecological seed package provided by the application is different from spraying pesticides, and the planted plants are a live and sustainable natural mosquito-repelling component factory, which can continuously and stably release effective components in a growing season and provide long-acting protection.

Description

Technical Field

[0001] This invention belongs to the field of pest control technology, specifically a multifunctional synergistic mosquito-repelling ecological seed pack and its application. Background Technology

[0002] As vectors of disease and essential to social development, mosquitoes not only cause skin inflammation through bites and bloodsucking, but also serve as vectors for diseases such as malaria, dengue fever, and Zika virus, posing a long-term threat to human health. To control mosquito damage, humans have successively developed various prevention and control methods, including physical barriers and chemical extermination. Among these, chemically synthesized repellents, represented by DEET, were once considered a major breakthrough, achieving broad-spectrum repellency (protection lasting 4-8 hours) by interfering with mosquitoes' olfactory receptors. However, recent studies have confirmed that DEET has significant drawbacks, including safety risks and environmental pollution. For example, it can penetrate the human skin barrier (24-hour absorption rate > 50%) and inhibit the activity of acetylcholinesterase (IC50) in the central nervous system. 50 =0.8μM), long-term exposure may lead to neurological damage, and DEET has a long degradation half-life, polluting water bodies and causing mosquitoes to develop resistance (resistance gene CYP4G19 expression is upregulated).

[0003] Against this backdrop, plant-derived repellents have once again become a research hotspot due to their environmentally friendly and low-toxicity properties. However, existing plant-based mosquito repellent technologies generally suffer from problems such as insufficient timeliness and limited scope of action.

[0004] Therefore, there is an urgent need to develop a multi-target synergistic, long-lasting, and eco-friendly mosquito repellent solution. This invention, through innovative plant combination strategies and seed treatment technology, constructs an ecological seed package that combines adult insect repellency, larval killing, and environmental restoration functions, providing a systematic technical path to overcome the limitations of chemical repellents and the shortcomings of plant technology. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention provides a multifunctional synergistic mosquito-repelling ecological seed pack and its application, as detailed below: A multifunctional synergistic mosquito-repelling ecological seed pack, wherein the ecological seed pack is a seed composition, the seed composition comprising repellent seeds, aromatic auxiliary seeds and long-lasting protective seeds, wherein the repellent seeds: aromatic auxiliary seeds: long-lasting protective seeds are in a mass ratio of 3-5:1-2:2-4; The repellent seeds are composed of lemongrass, pyrethrum, Elsholtzia ciliata, delphinium, geranium, rosemary and peppermint in equal proportions according to the number of seeds. The aromatic auxiliary seeds are composed of seeds of wild leek, scallion, sage, and catnip mixed in equal proportions according to the number of seeds. The long-lasting protective seed is composed of marigold, artemisia, gentian, calendula, and thyme seeds mixed in equal proportions according to the number of seeds.

[0006] Simply mix all the seeds thoroughly and pack them into seed packets at once; there is no need to pack them by type first.

[0007] Preferably, the ecological seed pack forms an odor barrier through volatile components to repel adult insects, and combines root secretions to kill larvae, thereby disrupting the mosquito breeding chain and achieving multifunctional synergistic mosquito repellency.

[0008] Preferably, the seeds are coated to form coated seeds.

[0009] Preferably, by weight ratio, the coated seeds contain 15-20 parts seeds, 16-20 parts microbial fertilizer, 60-70 parts substrate and 1-2 parts soil conditioner.

[0010] Preferably, by weight, the coated seeds comprise 16.67 parts seeds, 16.67 parts microbial fertilizer, 65.00 parts substrate, and 1.66 parts soil conditioner.

[0011] Preferably, the microbial fertilizer is a nitrogen-fixing bacterial fertilizer; the soil conditioner is a nitrogen-fixing bacteria and a phosphorus-solubilizing bacteria; the substrate is well-rotted sheep manure or cow manure, and the coating process uses low-temperature granulation technology to avoid the inactivation of live bacteria.

[0012] On the other hand, the present invention discloses the application of a multifunctional synergistic mosquito repellent ecological seed pack, wherein the ecological seed pack is sown at a sowing rate of 4.5-5.5 grams per square meter (based on the mass of pure plant seeds), and after sowing, it is covered with 1-2 centimeters of soil.

[0013] Furthermore, the sowing method involves row sowing along the regional boundaries or planting around water bodies.

[0014] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. The multifunctional synergistic mosquito repellent ecological seed pack provided by this invention completely eliminates chemically synthesized repellents such as DEET by constructing a system of all-plant-derived ingredients, thus avoiding the toxic risks to the human nervous system and skin irritation. At the same time, it avoids the long-term harm of chemical pollutants to water bodies and soil. Moreover, chemical repellents have a long degradation half-life, which easily leads to mosquito resistance. In contrast, this solution uses natural plant ingredients, which is significantly more environmentally friendly.

[0015] 2. The multifunctional synergistic mosquito-repelling ecological seed pack provided by this invention includes repellent seeds, aromatic auxiliary seeds, and long-lasting protective seeds. Repellent seeds, such as citronellal in citronella, pyrethrin in pyrethrum, and eucalyptol in rosemary, form an odor barrier that not only directly repels adult insects but also interferes with mosquito olfactory receptors. Aromatic auxiliary seeds, such as sage and catnip, release volatile components that have a certain repellency property and can modify, enhance, or mask the odor of repellent seeds, making the entire odor barrier more complex and variable, making it difficult for mosquitoes to recognize and become accustomed to them, thus delaying the development of resistance. Long-lasting protective seeds, such as marigold, artemisia, and thyme, have stable volatility and a long-lasting effect, forming a deep and persistent background odor layer that fills the gaps in concentration fluctuations during the volatilization process of other seeds. At the same time, marigold roots release α-terthiophene, and calendula roots secrete thiothiophene, which can kill larvae and block the mosquito breeding chain, solving the problem of the single-dimensional action of traditional plant repellents and achieving multi-target synergistic mosquito repellency.

[0016] 3. The multifunctional synergistic mosquito-repellent ecological seed pack provided by this invention is different from spraying agents. The plant grown is a living, sustainable natural mosquito-repellent ingredient factory that can continuously and stably release effective ingredients throughout a growing season, providing long-lasting protection. Detailed Implementation

[0017] Example 1 A multifunctional synergistic mosquito-repelling ecological seed pack, wherein the ecological seed pack is a seed composition, the seed composition comprising repellent seeds, aromatic auxiliary seeds and long-lasting protective seeds, wherein the repellent seeds: aromatic auxiliary seeds: long-lasting protective seeds = 3:1:2 by mass ratio; The repellent seeds are composed of lemongrass, pyrethrum, Elsholtzia ciliata, delphinium, geranium, rosemary and peppermint in equal proportions according to the number of seeds. The aromatic auxiliary seeds are composed of seeds of wild leek, scallion, sage, and catnip mixed in equal proportions according to the number of seeds. The long-lasting protective seed is composed of marigold, artemisia, gentian, calendula, and thyme seeds mixed in equal proportions according to the number of seeds.

[0018] Moreover, the ecological seed packs repel adult insects by forming an odor barrier through volatile components, and kill larvae by combining root secretions, thus disrupting the mosquito breeding chain and achieving multifunctional synergistic mosquito control.

[0019] Furthermore, the seeds are coated to form coated seeds.

[0020] Moreover, by weight ratio, the coated seeds contain 15 parts seeds, 16 parts microbial fertilizer, 70 parts substrate and 1 part soil conditioner.

[0021] Furthermore, the microbial fertilizer is a nitrogen-fixing bacterial fertilizer; the soil conditioner is composed of nitrogen-fixing bacteria and phosphorus-solubilizing bacteria; and the substrate is well-rotted sheep manure.

[0022] Example 2 A multifunctional synergistic mosquito-repellent ecological seed pack, wherein the ecological seed pack is a seed composition, the seed composition comprising repellent seeds, aromatic auxiliary seeds and long-lasting protective seeds, wherein the repellent seeds: aromatic auxiliary seeds: long-lasting protective seeds = 5:2:4 by mass ratio; The repellent seeds are composed of lemongrass, pyrethrum, Elsholtzia ciliata, delphinium, geranium, rosemary and peppermint in equal proportions according to the number of seeds. The aromatic auxiliary seeds are composed of seeds of wild leek, scallion, sage, and catnip mixed in equal proportions according to the number of seeds. The long-lasting protective seed is composed of marigold, artemisia, gentian, calendula, and thyme seeds mixed in equal proportions according to the number of seeds.

[0023] Moreover, the ecological seed packs repel adult insects by forming an odor barrier through volatile components, and kill larvae by combining root secretions, thus disrupting the mosquito breeding chain and achieving multifunctional synergistic mosquito control.

[0024] Furthermore, the seeds are coated to form coated seeds.

[0025] Moreover, according to the mass ratio, the coated seeds contain 20 parts seeds, 20 parts microbial fertilizer, 60 parts substrate and 2 parts soil conditioner.

[0026] Furthermore, the microbial fertilizer is a nitrogen-fixing bacterial fertilizer; the soil conditioner is composed of nitrogen-fixing bacteria and phosphorus-solubilizing bacteria; and the substrate is well-rotted cow manure.

[0027] Example 3 A multifunctional synergistic mosquito-repelling ecological seed pack, wherein the ecological seed pack is a seed composition, the seed composition comprising repellent seeds, aromatic auxiliary seeds and long-lasting protective seeds, wherein the repellent seeds: aromatic auxiliary seeds: long-lasting protective seeds = 4:1.5:3 by mass ratio; The repellent seeds are composed of lemongrass, pyrethrum, Elsholtzia ciliata, delphinium, geranium, rosemary and peppermint in equal proportions according to the number of seeds. The aromatic auxiliary seeds are composed of seeds of wild leek, scallion, sage, and catnip mixed in equal proportions according to the number of seeds. The long-lasting protective seed is composed of marigold, artemisia, gentian, calendula, and thyme seeds mixed in equal proportions according to the number of seeds.

[0028] Moreover, the ecological seed packs repel adult insects by forming an odor barrier through volatile components, and kill larvae by combining root secretions, thus disrupting the mosquito breeding chain and achieving multifunctional synergistic mosquito control.

[0029] Furthermore, the seeds are coated to form coated seeds.

[0030] Furthermore, by weight, the coated seeds contain 16.67 parts seeds, 16.67 parts microbial fertilizer, 65.00 parts substrate, and 1.66 parts soil conditioner.

[0031] Furthermore, the microbial fertilizer is a nitrogen-fixing bacterial fertilizer; the soil conditioner is a nitrogen-fixing bacteria and a phosphorus-solubilizing bacteria; and the substrate is well-rotted sheep manure and cow manure.

[0032] Example 4 The application of a multifunctional synergistic mosquito-repelling ecological seed pack involves sowing the ecological seed pack at a sowing rate of 4.5 grams per square meter (based on the mass of pure plant seeds), and then covering it with 1.5 centimeters of soil.

[0033] Moreover, the sowing method involves planting around the water area.

[0034] Moreover, when applied to community parks and planted around waterways, it helps to prevent mosquito breeding.

[0035] Example 5 The application of a multifunctional synergistic mosquito-repellent ecological seed pack involves sowing the ecological seed pack at a rate of 5.5 grams per square meter (based on the mass of pure plant seeds), and then covering it with 2 centimeters of soil.

[0036] Moreover, the sowing method involves planting around the water area.

[0037] Moreover, when applied to community parks and planted around waterways, it helps to prevent mosquito breeding.

[0038] Experimental Section Experiment 1 I. Experimental Materials Plant seeds: Seeds that repel pests: lemongrass, pyrethrum, Elsholtzia ciliata, delphinium, geranium, rosemary, and peppermint; Aromatic aid seeds: wild leek, scallion, sage, catnip; Long-lasting protective seeds: marigold, mugwort, fragrant orchid, calendula.

[0039] Experimental mosquitoes: 300 healthy adult Culex pipiens genus, Culex pipiens pallens, 3-5 days after emergence, and non-sucking females, artificially bred in the laboratory.

[0040] Experimental setup: Mosquito repulsion box (divided into multiple independent areas to count mosquito distribution).

[0041] II. Experimental Procedure and Results Experiment 1 1. Group planting The plant seeds of each group were sown in flower pots with a diameter of 20cm and a height of 18cm. The substrate was sterilized garden soil: well-rotted sheep manure = 3:1 (volume ratio). Each pot contained 1.5kg of soil, and the sowing amount was calculated according to the ratio in Example 3, which was 2g of pure seeds per pot.

[0042] All flowerpots were placed in an artificial climate incubator at a temperature of 26±1℃, relative humidity of 65±5%, light intensity of 16L:8D and light intensity of 2000±200 lux, until the leaves were fully expanded.

[0043] The control group used flowerpots of the exact same size and the same amount of substrate as the experimental group, placed them in adjacent positions in the same incubator, and received the same watering management, but did not sow any plants.

[0044] Experimental Group 1: Plant repellent seeds and aromatic auxiliary seeds, and cultivate them in the same environment until the leaves are fully expanded.

[0045] Experimental Group 2: Planting repellent seeds, aromatic auxiliary seeds, and long-lasting protective seeds, with cultivation conditions and planting density completely consistent with Experimental Group 1.

[0046] Control group: Blank areas without plants, serving as a reference for the natural distribution of mosquitoes.

[0047] 2. Mosquito release and observation Each group of flowerpots was placed in a separate area of ​​the repulsion box, ensuring uniform ventilation in all areas. One hundred healthy Culex mosquitoes were placed in the repulsion box, and the box was sealed. The number of mosquitoes in each area was counted every 30 minutes for 2 hours. The repulsion rate was calculated as: Repulsion rate = (Number of mosquitoes in the control group - Number of mosquitoes in the experimental group) / Number of mosquitoes in the control group × 100%.

[0048] 3. Experimental Results The avoidance rate of experimental group 1 was 85.6%; the avoidance rate of experimental group 2 was 95.6%, with the most significant effect; the avoidance rate of the control group was 0%, with no plant-based mosquito repellent effect and no obvious mosquito avoidance behavior.

[0049] Experiment 2 1. Group planting The experimental plant seeds were sown in the following groups, with 3 replicate flower pots in each group, each 25cm in diameter and filled with 2kg of soil, to eliminate individual differences.

[0050] Experimental Group 1: Aromatic auxiliary seeds and long-lasting protective seeds were planted and cultured in the same environment until the leaves were fully expanded. They were then mixed and sown according to the mass ratio calculated in Example 1.

[0051] Experimental Group 2: Planting repellent seeds, aromatic auxiliary seeds, and long-lasting protective seeds, with cultivation conditions and planting density completely consistent with Experimental Group 1, and mixed and sown according to the mass ratio calculated in Example 1.

[0052] Control group: The same amount of substrate from the same batch was filled into the flowerpots, and the same watering management was carried out. No plants were sown.

[0053] All flowerpots were placed in an artificial climate incubator, with the temperature controlled at 26±1℃, relative humidity at 65±5%, photoperiod at 16L:8D, and light intensity at 2000±200 lux. The soil was watered regularly to keep it moist. The plants were cultivated for about 4-6 weeks until the leaves of the plants in each experimental group were fully expanded.

[0054] 2. Mosquito release and observation Using the same mosquito release and observation methods as in Experiment 1, the avoidance rate was statistically analyzed and calculated.

[0055] 3. Experimental Results The avoidance rate of experimental group 1 was 81.2%; the avoidance rate of experimental group 2 was 96.2%, showing the best effect; the avoidance rate of the control group was 0%, indicating no mosquito-repelling effect of the plants.

[0056] Experiment 3 1. Group planting Experimental Group 1: Plant repellent seeds and long-lasting protective seeds, and cultivate them in the same environment until the leaves are fully expanded.

[0057] Experimental Group 2: Planting repellent seeds, aromatic auxiliary seeds, and long-lasting protective seeds, with cultivation conditions and planting density completely consistent with Experimental Group 1.

[0058] Control group: Blank area without plants.

[0059] 2. Mosquito release and observation Using the same mosquito release and observation methods as in Experiment 1, the avoidance rate was statistically analyzed and calculated.

[0060] 3. Experimental Results The avoidance rate of experimental group 1 was 90.3%; the avoidance rate of experimental group 2 was 94.8%, with the most significant effect; the avoidance rate of the control group was 0%, with no plant-based mosquito repellent effect.

[0061] Experiment 2 The ecological seeds from Example 3 were planted along the ponds and waterways of Hohhot Xiaocao Park at a rate of 5 grams per square meter. After sowing, the seeds were covered with 1-2 centimeters of soil and sown evenly in rows along the perimeter of the water area.

[0062] I. Methods for monitoring adult mosquito density The adult mosquito density was monitored using the mosquito-attracting lamp method as specified in the national standard GB / T23797-2020 "Methods for Monitoring Vector-borne Disease Density - Mosquitoes". The specific steps are as follows: Monitoring point layout: Three monitoring points were selected along the waterway in the planting area, with a distance of ≥50m between each monitoring point, avoiding interfering light sources such as streetlights. At the same time, a control point in a non-planting area was set up on the opposite bank of the waterway, 100m away from the planting area, for comparison.

[0063] Monitoring equipment: A carbon dioxide mosquito-attracting lamp was used, with a 6W ultraviolet fluorescent lamp as the light source, equipped with a small CO2 cylinder (release flow rate 200mL / min) as the attractant. The mosquito-attracting lamp was hung at a height of 1.5m above the ground.

[0064] For each monitoring session, the mosquito-attracting lamps were turned on one hour before sunset and turned off one hour after sunrise the following day. The captured mosquitoes were collected, with a trapping period of approximately 12 hours. Monitoring began approximately 6–8 weeks after sowing, after the tested plant population had entered a stable growth period (major functional leaves were fully expanded and overall plant development tended to be stable). The monitoring period was 21 days, conducted on days 0, 3, 6, 9, 12, 15, 18, and 21, for a total of 8 monitoring sessions. Each monitoring session was conducted at the same monitoring location, during the same time period, and using the same type of mosquito-attracting lamp.

[0065] The next day, after the lights were removed, the mosquitoes in the mosquito collection bag were placed in a -20°C freezer to kill them. After being removed, they were morphologically identified under a dissecting microscope and classified according to mosquito species. Only the number of female mosquitoes was counted (male mosquitoes do not suck blood and are not included in the density).

[0066] Calculate mosquito density: Mosquito density (number of mosquitoes / lamp / night) = Total number of female mosquitoes captured at the monitoring point ÷ Number of mosquito-attracting lamps; The final density of each monitoring point was taken as the average of three monitoring points and one control point to exclude interference from occasional factors.

[0067] II. Methods for Monitoring Bird Diversity A combination of transect and sampling methods was used to establish four fixed transects (each 2 km long) and six fixed observation points within a 100-meter radius of the planting area. Bird species and numbers were counted 1-3 hours after sunrise. Monitoring was conducted every 7 days for a total of 4 times: on days 0, 7, 14, and 21.

[0068] III. Monitoring Results A 21-day mosquito density monitoring study showed that the average adult mosquito density at the three monitoring sites in the planting area decreased sharply from an initial 11,650 mosquitoes / light / night to 198 mosquitoes / light / night on the 21st day, a drop of 98.3%. Meanwhile, the adult mosquito density at the control site decreased from 11,230 mosquitoes / light / night to 9,880 mosquitoes / light / night during the same period. This indicates that the planting of ecological seed packs was the direct cause of the significant decrease in mosquito density in the planting area.

[0069] Meanwhile, bird diversity increased from an initial 1.2 to 1.68, indicating that the application of this ecological seed package achieved the ecological goal of controlling mosquitoes without killing beneficial insects. Because no chemical pesticides were used and the plant community provided habitat and foraging grounds for birds, bird diversity increased simultaneously.

[0070] Experiment 3 In a residential community in Hohhot, a resident survey showed that 92% of respondents did not experience mosquito bites when staying in the green space for more than 30 minutes, and 87% of residents said that the mosquito-free environment significantly improved their living comfort.

Claims

1. A multifunctional, synergistic mosquito-repelling ecological seed packet, characterized in that, The ecological seed package is a seed composition, which contains repellent seeds, aromatic auxiliary seeds and long-lasting protective seeds, and the repellent seeds: aromatic auxiliary seeds: long-lasting protective seeds are in a mass ratio of 3-5:1-2:2-4. The repellent seeds are composed of lemongrass, pyrethrum, Elsholtzia ciliata, delphinium, geranium, rosemary and peppermint in equal proportions according to the number of seeds. The aromatic auxiliary seeds are composed of seeds of wild leek, scallion, sage, and catnip mixed in equal proportions according to the number of seeds. The long-lasting protective seed is composed of marigold, artemisia, gentian, calendula, and thyme seeds mixed in equal proportions according to the number of seeds.

2. The multifunctional synergistic mosquito-repelling ecological seed pack according to claim 1, characterized in that, Seeds are coated to form coated seeds.

3. The multifunctional synergistic mosquito-repelling ecological seed pack according to claim 2, characterized in that, According to the mass ratio, the coated seeds contain 15-20 parts seeds, 16-20 parts microbial fertilizer, 60-70 parts substrate and 1-2 parts soil conditioner.

4. The multifunctional synergistic mosquito-repelling ecological seed pack according to claim 3, characterized in that, By weight, the coated seeds comprise 16.67 parts seeds, 16.67 parts microbial fertilizer, 65.00 parts substrate, and 1.66 parts soil conditioner.

5. The multifunctional synergistic mosquito-repelling ecological seed pack according to claim 3, characterized in that, The microbial fertilizer is a nitrogen-fixing bacterial fertilizer; the soil conditioner is a nitrogen-fixing bacteria and a phosphorus-solubilizing bacteria; the substrate is well-rotted sheep manure and cow manure.

6. The application of a multifunctional synergistic mosquito-repelling ecological seed pack according to any one of claims 1-5, characterized in that, After sowing the ecological seed packets, cover them with 1-2 cm of soil.

7. The application according to claim 6, characterized in that, The sowing method involves row sowing along the regional boundaries or planting around water bodies.