High-throughput microfluidic analysis device

Abstract

The application provides a high-throughput microfluidic analysis device, comprising a shell; a conveying mechanism arranged in the shell and configured to carry and continuously convey a plurality of microfluidic chips; a conveying mechanism arranged below the conveying mechanism and configured to receive the microfluidic chips and move them to a detection position; a positioning mechanism arranged at the detection position and configured to fix the microfluidic chips; and a detection module arranged above the detection position and configured to collect color development data of the microfluidic chips. The high-throughput microfluidic analysis device of the application cooperates with the conveying mechanism, the conveying mechanism and the positioning mechanism to form a chip automatic flow pipeline, can sequentially convey the microfluidic chips to the detection position and accurately position them, and automatically collects the chips by a recycling mechanism after detection is completed, without manual shutdown and film replacement, thereby reducing the throughput bottleneck caused by intermittent operation and improving the continuous detection capacity of a single device.

Description

Technical Field

[0001] This invention relates to high-throughput microfluidic analysis equipment and belongs to the field of microfluidic detection technology. Background Technology

[0002] Microfluidic chip technology has attracted widespread attention in the field of rapid colorimetric detection of heavy metal ions in water due to its advantages such as low reagent consumption, high integration, and fast response. For example, by sequentially injecting reaction reagents such as copper ions, mercaptosuccinic acid, and nanocrystalline cellulose, ultracolloidal particles can be generated in situ within the chip, thereby enabling colorimetric identification of lead ions, silver ions, and other heavy metal ions. However, such detection procedures have strict requirements on the injection sequence, injection time, and mixing uniformity of various reaction reagents. If the timing is disrupted or cross-contamination occurs, the reliability of the detection results will be directly affected.

[0003] Currently, most commercially available microfluidic analysis devices operate intermittently. After each test, the chip needs to be manually removed and replaced, or the device needs to be paused to load a new chip. This prevents continuous, automated feeding, testing, and recycling of microfluidic chips, resulting in low overall throughput. In multi-reagent testing scenarios, operators typically need to manually add different reagents step by step. This process is not only cumbersome and inefficient, but repeated plugging and unplugging or switching of injection connectors can easily lead to reagent residues and cross-contamination, further reducing the stability and reproducibility of the tests. Therefore, how to achieve precise sequential injection and efficient mixing of multiple reagents in the same device, while continuously processing a large number of chips, has become a key technical problem that urgently needs to be solved in the field of high-throughput microfluidic analysis. To address this, a high-throughput microfluidic analysis device is proposed. Summary of the Invention

[0004] In view of this, the present invention provides a high-throughput microfluidic analysis device to solve or alleviate the technical problems of existing microfluidic analysis devices that use intermittent operation, have low detection throughput, and cannot achieve continuous automatic feeding, detection and recycling of chips, and at least provides a beneficial alternative.

[0005] The technical solution of the present invention is implemented as follows: a high-throughput microfluidic analysis device, including a housing; A conveying mechanism, disposed within the housing, is configured to carry and continuously convey multiple microfluidic chips; A conveying mechanism is disposed below the conveying mechanism and configured to receive the microfluidic chip and move it to the detection position; A positioning mechanism is provided at the detection position and configured to fix the microfluidic chip; A detection module is located above the detection position and is configured to collect colorimetric data from the microfluidic chip. The puncture and injection mechanism includes a puncture support, a linear drive module, a puncture slide, a needle tube assembly, and a valve core actuator. The valve core actuator is disposed on the puncture slide and its output end is connected to a multi-channel switching valve core. It is configured to insert the needle tube assembly into the microfluidic chip and inject the reaction reagents sequentially. A recycling mechanism is located downstream of the detection position and configured to recycle the microfluidic chip after detection. The microfluidic chip includes multiple sample inlets, a mixing channel, and a detection chamber.

[0006] More preferably, the conveying mechanism includes a conveying bracket, a first motor, a driving wheel, a driven wheel, and a synchronous conveyor belt. Chip carriers are evenly distributed on the synchronous conveyor belt, and the chip carriers are configured to support the microfluidic chip.

[0007] More preferably, the conveying mechanism includes a base, a second motor, a lead screw, and a slider, wherein the slider is threadedly connected to the lead screw, and a tray is fixedly connected above the slider.

[0008] More preferably, the positioning mechanism includes a linear drive module and a V-shaped positioning block, wherein the linear drive module drives the V-shaped positioning block to clamp or release the microfluidic chip located at the detection position.

[0009] More preferably, the microfluidic chip is provided with a first liquid storage tank, a second liquid storage tank and a third liquid storage tank, and the first liquid storage tank, the second liquid storage tank and the third liquid storage tank are all connected to the sample inlet.

[0010] More preferably, the multi-channel switching valve core includes a first sealing layer, a second sealing layer and a third sealing layer, wherein the first sealing layer is configured to close all sample inlets, the second sealing layer is configured to connect the first liquid storage tank and the second liquid storage tank, and the third sealing layer is configured to connect the second liquid storage tank and the third liquid storage tank.

[0011] More preferably, the first sealing layer is 0.5 mm away from the bottom of the multi-channel switching valve core, the second sealing layer is 1.5 mm away from the bottom of the valve core, and the third sealing layer is 2.5 mm away from the bottom of the valve core.

[0012] More preferably, the mixing channel of the microfluidic chip includes, in sequence, a right-angled bend channel, a first annular channel, an arc mixing channel, a first arc channel, a second arc channel, a second annular channel, a first right-angle channel, a third arc channel, a third arc channel, a third annular channel, a second right-angle channel, a fourth arc channel, a flow splitting and merging channel, an annular bend channel, and a detection chamber.

[0013] More preferably, the recycling mechanism includes a recycling bin and a pusher rod, the tray has a notch, and the pusher rod is configured to push the detected microfluidic chip into the recycling bin through the notch.

[0014] More preferably, the microfluidic chip is made of polydimethylsiloxane and has dimensions of 50 mm in length, 30 mm in width, and 1 mm in height, and the cross-sectional dimensions of the mixing channel are 0.4 mm in width and 0.4 mm in depth.

[0015] The embodiments of the present invention have the following advantages due to the adoption of the above technical solutions: The high-throughput microfluidic analysis equipment of the present invention forms an automated chip flow line through the coordinated operation of a conveying mechanism, a transport mechanism and a positioning mechanism. It can sequentially transport microfluidic chips to the testing station and accurately position them. After the testing is completed, the chip is automatically collected by the recycling mechanism, eliminating the need for manual shutdown and chip replacement. This reduces the throughput bottleneck caused by intermittent operation and improves the continuous testing capability of a single device.

[0016] The puncture and injection mechanism of this invention is linked with a multi-channel switching valve core. While the positioning mechanism locks the chip, it can sequentially puncture the chip injection port and switch different reagent channels according to a strict timing control, thereby realizing the sequential injection of multiple reaction reagents. The multi-channel switching valve core relies on a specific sealing layer structure to effectively prevent cross-flow between reagents during the switching and holding of different channels, eliminating cross-contamination. This allows reagents such as lead ions, silver ions, and ultracolloidal particles to stably enter the mixing region according to the required reaction timing, laying the foundation for a reliable subsequent colorimetric reaction.

[0017] The above overview is for illustrative purposes only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features of the invention will become readily apparent from the accompanying drawings and the following detailed description. Attached Figure Description To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a structural diagram of the present invention.

[0019] Figure 2 This is a structural diagram of the transmission support and reagent carrier in this invention.

[0020] Figure 3 This is a structural diagram of the conveying mechanism and transport mechanism in this invention.

[0021] Figure 4 This is a structural diagram of the synchronous transmission belt and chip carrier in this invention.

[0022] Figure 5 This is a structural diagram of the tray and positioning clamping mechanism in this invention.

[0023] Figure 6 This is a structural diagram of the detection module and the recycling mechanism in this invention.

[0024] Figure 7 For the present invention Figure 6 Enlarged diagram of area A in the middle.

[0025] Figure 8 This is a structural diagram of the guide groove and the extrusion plate in this invention.

[0026] Figure 9 This is a structural diagram of the syringe and needle tubing assembly in this invention.

[0027] Figure 10 This is a structural diagram of the microfluidic chip in this invention.

[0028] Figure 11 This is a diagram showing the internal structure of the multi-channel switching valve core in this invention.

[0029] Figure 12 This is a top view of the multi-channel switching valve core in this invention.

[0030] in: 1-Shell; 10-Cover plate; 11-Through groove; 12-Reagent carrier rack; 13-Detection mounting rack; 14-Transfer bracket; 2-Microfluidic chip; 20-a First reservoir; 20-b Second reservoir; 20-c Third reservoir; 21-Right-angle bend channel; 22-a First annular channel; 23-Circular arc mixing channel; 24-First circular arc channel; 25-a Second circular arc channel; 22-b Second annular channel; 26-a First right-angle channel; 27-Third circular arc channel; 25-b Fourth circular arc channel; 22-c Third annular channel; 26-b First right-angle channel; 28-Fifth circular arc channel; 29-Split and merge channel; 201-Annular bend channel; 202-Detection chamber; 203-Inlet; 204-Valve chamber; 205-Multi-channel switching valve core; 205-a First inlet channel; 205-b Second inlet channel; 205 -c Third liquid inlet channel; 2051 First sealing layer; 2052 Second sealing layer; 2053 Third sealing layer; 3 Conveying mechanism; 30 First motor; 31 Driving wheel; 32 Driven wheel; 33 Synchronous conveyor belt; 34 Chip carrier; 4 Conveying mechanism; 40 Base; 41 Second motor; 42 Lead screw; 43 Slider; 44 Tray; 45 Notch; 5 Detection module; 51 Camera; 52 Linear drive module; 6 Positioning mechanism; 63 Guide rod; 64 V-shaped positioning block; 65 Auxiliary support block; 7 Injection pump assembly; 71 Guide groove; 73 Squeezing plate; 74 Syringe; 76 Needle tubing assembly; 8 Puncture drive mechanism; 80 Puncture bracket; 82 Puncture slide; 83 Needle guide hole; 84 Valve core driver; 9 Recovery mechanism; 90 Recovery chamber; 91 Push rod. Detailed Implementation To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. However, it should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of the invention. Furthermore, descriptions of well-known structures and technologies are omitted in the following description to avoid unnecessarily obscuring the concept of the invention.

[0031] In the description of this invention, it should be noted that when an element is referred to as being "fixed to" or "set on" another element, it can be directly on or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to or indirectly connected to the other element.

[0032] In the description of this invention, it should be noted that the terms "center," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product of this invention is in use. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly specified. "Several" means one or more, unless otherwise explicitly specified.

[0033] In the description of this invention, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0034] The embodiments of the present invention will now be described in detail with reference to the accompanying drawings.

[0035] like Figure 1-12 As shown, this embodiment of the invention provides a high-throughput microfluidic analysis device, comprising: a housing 1; a conveying mechanism 3, disposed within the housing 1, configured to carry and continuously convey multiple microfluidic chips 2; a conveying mechanism 4, disposed below the conveying mechanism 3, configured to receive the microfluidic chips 2 and move them to a detection position; a positioning mechanism 6, disposed at the detection position, configured to fix the microfluidic chips 2; a detection module 5, disposed above the detection position, configured to collect colorimetric data from the microfluidic chips 2; a puncture injection mechanism 8, comprising a puncture support 80, a linear drive module 52, a puncture slide 82, a needle-tube assembly 76, and a valve core actuator 84, wherein the valve core actuator 84 is disposed on the puncture slide 82 and its output end is connected to a multi-channel switching valve core 205, configured to insert the needle-tube assembly 76 into the microfluidic chips 2 and sequentially inject reaction reagents; and a recovery mechanism 9, disposed downstream of the detection position, configured to recover the microfluidic chips 2 after detection; wherein the microfluidic chips 2 include multiple injection ports 203, a mixing channel, and a detection chamber 202.

[0036] In one embodiment, see Figures 1 to 5 The conveying mechanism 3 includes a conveying bracket 14, a first motor 30, a driving wheel 31, a driven wheel 32, and a synchronous conveyor belt 33. Chip carriers 34 are equidistantly distributed on the synchronous conveyor belt 33, and the chip carriers 34 are configured to support the microfluidic chip 2. The conveying mechanism 4 includes a base 40, a second motor 41, a lead screw 42, and a slider 43. The slider 43 is threadedly connected to the lead screw 42, and a tray 44 is fixedly connected above the slider 43. Two first motors 30 are installed inside the conveying bracket 14. The output ends of both first motors 30 are connected to the driving wheel 31, and the other end of the driving wheel 31 rotates on the conveying bracket 14. Multiple driven wheels 32 are arranged above each driving wheel 31. A synchronous conveyor belt 33 is installed on each side of the driving wheel 31 and driven wheel 32. The chip carriers 34, equidistantly distributed on the synchronous conveyor belt 33, are symmetrically arranged in pairs to support the microfluidic chip 2. By starting the first motor 30, the driving wheel 31 is driven to rotate the synchronous conveyor belt 33, releasing microfluidic energy at the bottom. The microfluidic chip 2 is placed into the tray 44 on the conveying mechanism 4; and a through slot 11 is provided at the corresponding position of the housing 1 to facilitate the placement of the microfluidic chip 2; the pulley diameter of the synchronous conveyor belt 33 is 32mm, the distance between the driving wheel 31 and the driven wheel 32 is 66.6mm, the distance between the left and right synchronous conveyor belts 33 is 31mm, and the distance between the upper and lower chip carriers 34 is 12mm. A maximum of 14 microfluidic chips 2 can be stored at one time; when in use, the first motor 30 drives the synchronous conveyor belt 33 to convey 12mm at a time, delivering one microfluidic chip 2 to the tray 44.

[0037] The conveying mechanism 4 is a moving mechanism connecting the conveying mechanism 3 and the detection position. It moves the slider 43 and the microfluidic chip 2, which is supported on the tray 44, to the detection position by activating the second motor 41. The base 40 has a total length of 220mm, and the slider 43 can move 171mm. The tray 44 receives the microfluidic chip 2 conveyed by the synchronous conveyor belt 33, delivers it to the detection position, pauses, and proceeds to the next detection step.

[0038] In one embodiment, see Figure 6 The positioning mechanism 6 includes a linear drive module 52 and a V-shaped positioning block 64. The linear drive module 52 drives the V-shaped positioning block 64 to clamp or release the microfluidic chip 2 located at the detection position. The linear drive module 52 adopts a ball screw structure, which is a well-known linear drive mechanism, and its working principle will not be described in detail here. The drive end of the linear drive module 52 drives the V-shaped positioning block 64 through the guide rod 63 to position and clamp the microfluidic chip 2 on the tray 44. The movement and extension distance of the V-shaped positioning block 64 are controlled by controlling the motor.

[0039] In one embodiment, see Figures 6-9The puncture drive module 8 includes a puncture support 80, a linear drive module 52, and a puncture slide 82. The second linear module 52 drives the puncture slide 82 to insert or withdraw the needle tubing assembly 76 into or out of the injection port 203. A reagent carrier 12 is also installed inside the housing 1. The linear drive module 52 is installed on one side of the reagent carrier 12, and a guide groove 71 is provided on the reagent carrier 12. The drive end of the linear drive module 52 is connected to a squeeze plate 73. An injection pump assembly 7 is installed on the other side of the reagent carrier 12. The injection pump assembly 7 consists of three syringes 74 arranged in a row. The bottom of each syringe 74 is connected to the needle tubing assembly 76. The squeeze plate 73 passes through the guide groove 71 and rests above the syringes 74. The drive end of the linear drive module 52 squeezes the syringes 74 through the squeeze plate 73 to inject liquid, allowing three 50mL syringes 74 to simultaneously inject liquid into the needle tubing assembly 76.

[0040] A linear drive module 52 is installed on the puncture support 80. The drive end of the linear drive module 52 is connected to the puncture slide 82. The puncture slide 82 has a needle guide hole 83. One end of the needle tube assembly 76 is fixed to the puncture slide 82. The needle of the needle tube assembly 76 is inserted into the needle guide hole 83 for positioning. The linear drive module 52 drives the puncture slide 82 to move, thereby driving the needle of the needle tube assembly 76 to insert into or pull out of the injection port 203 on the microfluidic chip 2 for liquid injection.

[0041] In one embodiment, see Figure 7 , Figures 11-12 The puncture slide 82 is also equipped with a valve core actuator 84. The output of the valve core actuator 84 is connected to a multi-channel switching valve core 205, which is configured to control the on / off state and mixing sequence of different injection ports 203. The valve core actuator 84 is an electric actuator. When the microfluidic chip 2 enters the detection position, the valve core actuator 84 can move down together with the puncture slide 82. The valve core actuator 84 is inserted into the valve cavity 204 on the microfluidic chip 2.

[0042] In one embodiment, see Figure 10The microfluidic chip 2 is provided with a first liquid reservoir 20a, a second liquid reservoir 20b, and a third liquid reservoir 20c, and each of the first liquid reservoir 20a, the second liquid reservoir 20b, and the third liquid reservoir 20c is connected to an inlet 203. The mixing channel of the microfluidic chip 2 includes a right-angle bent channel 21, a first annular channel 22a, an arc mixing channel 23, a first arc channel 24, a second arc channel 25a, a second annular channel 22b, a first right-angle channel 26a, a third arc channel 27, a third arc channel 25b, a third annular channel 22c, a second right-angle channel 26b, a fourth arc channel 28, a splitting and merging channel 29, an annular bent channel 201, and a detection chamber 202. The microfluidic chip 2 is made of polydimethylsiloxane and has dimensions of 50 mm in length, 30 mm in width, and 1 mm in height. The cross-sectional dimensions of the mixing channel are 0.4 mm in width and 0.4 mm in depth. The first reservoir 20a, the second reservoir 20b, and the third reservoir 20c in the microfluidic chip 2 have an arc radius of 2mm and a tangent length of 5mm. The sample inlet 203 is 0.2mm. Lead ion reagent, ultracolloidal particle reagent, and silver ion reagent are injected into the first reservoir 20a, the second reservoir 20b, and the third reservoir 20c, respectively, and then enter the multi-channel switching valve core 205. The fluid initially mixes through a right-angle bend channel 21 (0.4 mm wide) and enters a first annular channel 22a (1.6 mm inner diameter) for further mixing. It then flows through a circular arc mixing channel 23 (0.22 mm inner diameter), through a first circular arc channel 24 (2.42 mm inner diameter), and a second circular arc channel 25a (1.68 mm inner diameter) before entering a second annular channel 22b with the same structure for further mixing. It then flows through a first right-angle channel 26a, through a third circular arc channel 27 (3.56 mm inner radius), and then into a fourth circular arc channel 25b, a third annular channel 22c, and a second right-angle channel 26b for mixing. Afterward, it flows through a fifth circular arc channel 28 (1.45 mm inner radius) and enters a final merging and splitting channel 29 (0.6 mm inner diameter). Finally, it is mixed through an annular bend channel 201 (2.4 mm inner diameter) and enters a detection chamber 202 (16 mm long, 7 mm wide).

[0043] In one embodiment, see Figure 7 , Figures 11-12 The multi-channel switching valve core 205 includes a first sealing layer 2051, a second sealing layer 2052, and a third sealing layer 2053. The first sealing layer 2051 is configured to close all sample inlets 203. The second sealing layer 2052 is configured to connect the first liquid storage tank 20a and the second liquid storage tank 20b. The third sealing layer 2053 is configured to connect the second liquid storage tank 20b and the third liquid storage tank 20c. The first sealing layer 2051 is 0.5 mm away from the bottom of the valve core of the multi-channel switching valve core 205, the second sealing layer 2052 is 1.5 mm away from the bottom of the valve core, and the third sealing layer 2053 is 2.5 mm away from the bottom of the valve core.

[0044] The puncture and injection mechanism 8 works in conjunction with the multi-channel switching valve core 205. The valve core driver 84 drives the multi-channel switching valve core 205 to move axially within the valve cavity 204 of the microfluidic chip 2. By switching the axial positions of the first sealing layer 2051, the second sealing layer 2052, and the third sealing layer 2053, the first liquid reservoir 20a, the second liquid reservoir 20b, and the third liquid reservoir 20c are sequentially connected and isolated, preventing cross-contamination of different reagents before injection.

[0045] The multi-channel switching valve core 205 is divided into a first sealing layer 2051, a second sealing layer 2052, and a third sealing layer 2053. When the needle of the puncture slide 82 moves and drives the needle of the tubing assembly 76 to insert into the corresponding injection port 203, it drives the multi-channel switching valve core 205 on the valve core driver 84 to move and insert into the valve chamber 204, ensuring that the first sealing layer 2051 seals the liquid in the first liquid storage tank 20a, the second liquid storage tank 20b, and the third liquid storage tank 20c. The second sealing layer is provided with a first liquid inlet channel 205a and a second liquid inlet channel 205b. One end of the first liquid inlet channel 205a is connected to the first liquid storage tank 20a, and one end of the second liquid inlet channel 205b is connected to the second liquid storage tank 20b. The other ends of the first liquid inlet channel 205a and the second liquid inlet channel 205b are connected side by side to the right-angle bend channel. 21. Mixing: The third sealing layer 2053 is provided with a second liquid inlet channel 205b and a third liquid inlet channel 205c. One end of the second liquid inlet channel 205b is connected to the second liquid storage tank 20b, and one end of the third liquid inlet channel 205c is connected to the third liquid storage tank 20c. The second liquid inlet channel 205b and the third liquid inlet channel 205c are connected in parallel and enter the right-angle bend channel 21 for mixing. After injection, the valve core actuator 84 drives the multi-channel switching valve core 205 to the second sealing layer 2052 to mix the first liquid storage tank 20a and the second liquid storage tank 20b, and blocks the third liquid storage tank 20c. After mixing, the valve core actuator 84 drives the multi-channel switching valve core 205 to the third sealing layer 2053 to mix the second liquid storage tank 20b and the third liquid storage tank 20c, and blocks the first liquid storage tank 20a. Different detection liquids are mixed through multiple channels.

[0046] In one embodiment, see Figure 6 The recycling mechanism 9 includes a recycling chamber 90 and a pusher rod 91. A notch 45 is provided on the tray 44. The pusher rod 91 is configured to pass through the notch 45 to push the tested microfluidic chip 2 into the recycling chamber 90. The pusher rod 91 is an electric pusher rod. The tested microfluidic chip 2 is transported to the recycling chamber 90 by the conveying mechanism 4. The pusher rod 91 passes through the notch 45 to push the tested microfluidic chip 2 into the recycling chamber 90. A detection mounting bracket 13 is also installed inside the housing 1. The detection module 5 is located on the mounting bracket 13. The detection module 5 includes a linear drive module 52 and a camera 51. The linear drive module 52 is configured to adjust the lateral distance between the camera 51 and the detection chamber 202 for fine-tuning the acquisition position.

[0047] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any person skilled in the art can easily conceive of various variations or substitutions within the technical scope disclosed in the present invention, and these should all be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A high-throughput microfluidic analysis device, characterized in that, include: Shell (1); The conveying mechanism (3) is disposed inside the housing (1) and configured to carry and continuously convey multiple microfluidic chips (2). The conveying mechanism (4) is located below the conveying mechanism (3) and is configured to receive the microfluidic chip (2) and move it to the detection position; A positioning mechanism (6) is set at the detection position and configured to fix the microfluidic chip (2). The detection module (5) is located above the detection position and is configured to collect the colorimetric data of the microfluidic chip (2); The puncture injection mechanism (8) includes a puncture support (80), a linear drive module (52), a puncture slide (82), a needle tube assembly (76), and a valve core actuator (84). The valve core actuator (84) is disposed on the puncture slide (82) and its output end is connected to a multi-channel switching valve core (205). It is configured to insert the needle tube assembly (76) into the microfluidic chip (2) and inject the reaction reagents sequentially. A recycling mechanism (9) is located downstream of the detection position and is configured to recycle the microfluidic chip (2) after detection. The microfluidic chip (2) includes multiple sample inlets (203), a mixing channel, and a detection chamber (202).

2. The high-throughput microfluidic analysis device according to claim 1, characterized in that: The conveying mechanism (3) includes a conveying bracket (14), a first motor (30), a driving wheel (31), a driven wheel (32), and a synchronous conveyor belt (33). Chip carriers (34) are evenly distributed on the synchronous conveyor belt (33), and the chip carriers (34) are configured to carry the microfluidic chip (2).

3. The high-throughput microfluidic analysis device according to claim 1, characterized in that: The conveying mechanism (4) includes a base (40), a second motor (41), a lead screw (42) and a slider (43). The slider (43) is threadedly connected to the lead screw (42), and a tray (44) is fixedly connected above the slider (43).

4. The high-throughput microfluidic analysis device according to claim 1, characterized in that: The positioning mechanism (6) includes a linear drive module (52) and a V-shaped positioning block (64). The linear drive module (52) drives the V-shaped positioning block (64) to clamp or release the microfluidic chip (2) located at the detection position.

5. The high-throughput microfluidic analysis device according to claim 1, characterized in that: The microfluidic chip (2) is provided with a first liquid storage tank (20a), a second liquid storage tank (20b) and a third liquid storage tank (20c), and the first liquid storage tank (20a), the second liquid storage tank (20b) and the third liquid storage tank (20c) are all connected to the sample inlet (203).

6. The high-throughput microfluidic analysis device according to claim 1, characterized in that: The multi-channel switching valve core (205) includes a first sealing layer (2051), a second sealing layer (2052), and a third sealing layer (2053). The first sealing layer (2051) is configured to close all sample inlets (203), the second sealing layer (2052) is configured to connect the first liquid storage tank (20a) and the second liquid storage tank (20b), and the third sealing layer (2053) is configured to connect the second liquid storage tank (20b) and the third liquid storage tank (20c).

7. The high-throughput microfluidic analysis device according to claim 6, characterized in that: The first sealing layer (2051) is 0.5 mm away from the bottom of the valve core of the multi-channel switching valve core (205), the second sealing layer (2052) is 1.5 mm away from the bottom of the valve core, and the third sealing layer (2053) is 2.5 mm away from the bottom of the valve core.

8. The high-throughput microfluidic analysis device according to claim 1, characterized in that: The microfluidic chip (2) includes a right-angle bend channel (21), a first annular channel (22a), an arc mixing channel (23), a first arc channel (24), a second arc channel (25a), a second annular channel (22b), a first right-angle channel (26a), a third arc channel (27), a fourth arc channel (25b), a third annular channel (22c), a second right-angle channel (26b), a fifth arc channel (28), a flow splitting and merging channel (29), an annular bend channel (201), and a detection chamber (202).

9. The high-throughput microfluidic analysis device according to claim 3, characterized in that: The recycling mechanism (9) includes a recycling bin (90) and a pusher (91). A notch (45) is provided on the tray (44). The pusher (91) is configured to push the tested microfluidic chip (2) into the recycling bin (90) through the notch (45).

10. The high-throughput microfluidic analysis device according to claim 1, characterized in that: The microfluidic chip (2) is made of polydimethylsiloxane and has dimensions of 50 mm in length, 30 mm in width, and 1 mm in height. The cross-sectional dimensions of the mixing channel are 0.4 mm in width and 0.4 mm in depth.

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