Inverted microscope cell culture flask tilting swivel adapter

Abstract

The application relates to the technical field of microscope auxiliary observation equipment, and relates to a tilting type rotating adapter for a cell culture bottle of an inverted microscope, which comprises a fixed support, a mounting rack arranged on the fixed support, a tilting adjusting mechanism arranged on the mounting rack, a fine adjustment speed reducer fixed on the top of the mounting rack, and a fine adjustment knob arranged on the input end of the fine adjustment speed reducer; the centering and clamping mechanism adopts a driving motor as a single power source, can automatically complete the two actions of bottle opening clamping and bottle body alignment and pressing in sequence through the cooperation of a driving rod, a driving sleeve, a fixing sleeve and a guide protrusion and a guide slide, can be adapted to T-shaped cell culture bottles of different specifications, has a wide adaptation range, can guarantee the posture consistency of the T-shaped cell culture bottle after each clamping, can keep the initial tilting direction of the observation surface of the bottle bottom stable, effectively reduces the clamping and debugging difficulty, and improves the observation consistency.

Description

Technical Field

[0001] This invention belongs to the technical field of microscope auxiliary observation equipment, specifically relating to a tilting rotary adapter for cell culture flasks used in inverted microscopes. Background Technology

[0002] Inverted microscopes are core observation equipment in cell biology research, biomedical detection, and other fields. Their objectives are located below the stage and the light source illuminates from above, allowing observation of adherent cells from the bottom of the sample upwards. This effectively avoids direct contact between the objectives and the culture medium, meeting the core needs of in vivo cell observation. They are widely used in scenarios such as morphological observation, proliferation counting, and activity detection of adherent cells in cell culture flasks. T-type cell culture flasks, as the mainstream carrier for large-scale culture of adherent cells, have the characteristics of large culture area, stable adhesion effect, and offset mouth design for easy liquid addition and sampling. They are highly compatible with the observation principle of inverted microscopes and are the preferred culture container for observation under inverted microscopes.

[0003] Existing inverted microscope stages can only achieve X and Y axis translation and Z axis focusing, lacking a dedicated tilting positioning mechanism for T-type cell culture flasks. This not only makes it difficult to accurately control the tilt angle, but also requires readjustment after each change of T-type cell culture flask, making the operation cumbersome and inefficient. Although some simple adapters can achieve tilting, they lack a reliable positioning structure, making it easy for the flask to wobble and the angle to shift during observation. Moreover, it is impossible to reproduce a uniform tilt angle during batch observation, resulting in inconsistent observation conditions for different samples, affecting the accuracy and repeatability of experimental data. Therefore, it is necessary to design a tilting rotation adapter for cell culture flasks used in inverted microscopes. Summary of the Invention

[0004] The purpose of this invention is to provide a simple and reasonably designed tilting rotary adapter for cell culture flasks used in inverted microscopes in order to solve the above-mentioned problems.

[0005] The present invention achieves the above objectives through the following technical solutions:

[0006] A tilting rotary adapter for cell culture flasks used in inverted microscopes includes a fixed support with a mounting frame on the fixed support. The mounting frame has a tilt adjustment mechanism, which includes a fine-tuning gearbox fixed to the top of the mounting frame. The input end of the fine-tuning gearbox has a fine-tuning knob, and the output end of the fine-tuning gearbox has a docking frame fixed. An alignment ring is slidably connected to the docking frame, and a push frame is rotatably connected to the alignment ring. The push frame is fixedly connected to the output end of a docking electric cylinder, which is fixed to the fine-tuning gearbox. The alignment ring abuts against a driven ring, which is fixed to a connecting frame. A rotating frame is rotatably connected to the connecting frame and rotatably connected to a rotating base. A centering clamping mechanism is fixed to the connecting frame, including a side support fixed to the connecting frame. A flask mouth clamping mechanism is provided on the side support, and a driving mechanism is provided at the bottom of the connecting frame, connected to a downward centering mechanism.

[0007] As a further optimization of the present invention, the driven ring is symmetrically provided with alignment grooves, and the alignment ring is symmetrically provided with alignment protrusions that abut against the alignment grooves.

[0008] As a further optimization of the present invention, the driven ring is provided with a quick reset mechanism, the quick reset mechanism including a driven sleeve fixed in the driven ring, and a first magnet is nested in a groove symmetrically opened on the side wall of the driven sleeve.

[0009] As a further optimization of the present invention, an active sleeve is fixed on the docking frame, and a second magnet that attracts the first magnet is nested in a groove symmetrically opened on the side wall of the active sleeve.

[0010] As a further optimization of the present invention, the bottle mouth clamping mechanism includes an upper clamping block symmetrically slidably connected to the side support, and a limiting post slidably connected in a through groove opened on the upper clamping block, the limiting post being symmetrically fixed to the top of the clamping plate.

[0011] As a further optimization of the present invention, the driving mechanism includes a driving motor fixed to the bottom of the connecting frame, and a square driving rod fixed to the output end of the driving motor, the driving rod being slidably connected in a square groove at the bottom of the driving sleeve.

[0012] As a further optimization of the present invention, a guide protrusion is provided on the side wall of the drive sleeve, the drive sleeve is slidably connected in the fixed sleeve, the guide slide is opened in the inner wall of the fixed sleeve and is slidably connected to the guide protrusion, the fixed sleeve is rotatably connected to the connecting frame, and the clamping disk is fixedly sleeved on the fixed sleeve.

[0013] As a further optimization of the present invention, the pressing centering mechanism includes an upper support rotatably connected to the drive sleeve, a lower support fixed to the connecting frame and slidably connected to the bottom of the upper support, a central rod slidably connected to the upper support on the connecting frame, and a support spring between the upper support and the connecting frame, the support spring being sleeved on the central rod.

[0014] As a further optimization of the present invention, a lifting frame is slidably connected to the connecting frame, the lifting frame is fixed to the top of the upper support, and V-shaped frames are rotatably connected to both sides of the lifting frame. An upper cover plate is fixed on the V-shaped frame, and pressure rollers located inside the upper cover plate are symmetrically fixed on the V-shaped frame.

[0015] As a further optimization of the present invention, the rotating seat is fixed on the rotating support, and both the rotating support and the fixed support are fixed on the microscope stage.

[0016] The beneficial effects of this invention are as follows:

[0017] 1. The centering clamping mechanism of this invention uses a drive motor as a single power source. Through the cooperation of the drive rod, drive sleeve, fixed sleeve, guide protrusion and guide slide, it can automatically complete the two actions of clamping the bottle mouth and aligning and pressing the bottle body in sequence. When clamping T-type cell culture flasks, the upper clamping block first centers and clamps the bottle mouth, and then the pressure roller presses and positions the top inclined surface of the bottle body, so that the cell flask and the side support remain parallel. It can be adapted to T-type cell culture flasks of different specifications and has a wide range of compatibility. At the same time, it can ensure that the posture of the T-type cell culture flask is consistent after each clamping, so that the initial tilt direction of the bottom observation surface of the flask remains stable, effectively reducing the difficulty of clamping and debugging and improving the consistency of observation.

[0018] 2. This invention achieves a close fit and positioning by driving the alignment ring and driven ring with a docking electric cylinder. Based on the magnetic coarse positioning, the alignment protrusion and alignment groove interlock to form a rigid constraint, which can completely restrict circumferential rotation and radial sway. During tilt observation, the alignment ring and driven ring always maintain a tight contact, avoiding tilt angle deviation caused by gaps or looseness, ensuring the stability of the cell bottle's posture during observation, thereby obtaining a clear and stable imaging effect and improving the reliability of observation and the accuracy of data.

[0019] 3. This invention sets up paired magnets in the active sleeve and the driven sleeve to form a quick reset mechanism. After the first observation is completed and the fine adjustment knob is locked, when the culture bottle is replaced, the rotating frame can automatically achieve circumferential coarse positioning under the action of magnetic force after reset, so that the alignment groove and the alignment protrusion are quickly aligned. Then, the fine positioning and locking are completed by the electric cylinder. The previous observation posture can be reproduced with high precision without the need to readjust the tilt angle, which greatly shortens the clamping and debugging time and significantly improves the efficiency of batch detection. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0021] Figure 2 This is a schematic diagram showing the position of the tilt adjustment mechanism in this invention;

[0022] Figure 3 This is an assembly diagram of the tilt adjustment mechanism in this invention;

[0023] Figure 4 yes Figure 3 A magnified view of a portion of region A in the middle;

[0024] Figure 5 This is a schematic diagram showing the position of the rapid reset mechanism in this invention;

[0025] Figure 6 This is a schematic diagram of the downward pressing centering mechanism in this invention;

[0026] Figure 7 This is a schematic diagram of the centering clamping mechanism in this invention;

[0027] Figure 8 This is an assembly diagram of the centering clamping mechanism in this invention;

[0028] Figure 9 This is a schematic diagram of the drive mechanism in this invention;

[0029] Figure 10 This is a schematic diagram of the connecting frame in this invention.

[0030] In the diagram: 1. Fixed support; 2. Mounting bracket; 3. Tilting adjustment mechanism; 4. Connecting bracket; 5. Rotating bracket; 6. Rotating seat; 7. Centering clamping mechanism; 8. Rotating support; 31. Fine-tuning gearbox; 32. Fine-tuning knob; 33. Docking bracket; 34. Alignment ring; 35. Pushing bracket; 36. Docking electric cylinder; 37. Driven ring; 38. Quick reset mechanism; 71. Side support; 72. Bottle neck clamping mechanism; 73. Drive mechanism; 74. Downward centering mechanism; 371. Alignment groove; 372. Alignment protrusion 381. Driven sleeve; 382. First magnet; 383. Driving sleeve; 384. Second magnet; 721. Upper clamping block; 722. Limiting post; 723. Clamping plate; 731. Drive motor; 732. Drive rod; 733. Drive sleeve; 734. Guide protrusion; 735. Fixed sleeve; 736. Guide slide; 741. Upper support; 742. Lower support; 743. Center rod; 744. Support spring; 745. Lifting frame; 746. V-shaped frame; 747. Pressure roller; 748. Upper cover plate. Detailed Implementation

[0031] The present application will now be described in further detail with reference to the accompanying drawings. It should be noted that the following specific embodiments are only used to further illustrate the present application and should not be construed as limiting the scope of protection of the present application. Those skilled in the art can make some non-essential improvements and adjustments to the present application based on the above application content.

[0032] Example: Please refer to Figures 1-10A tilting and rotating adapter for cell culture flasks used in an inverted microscope includes a fixed support 1 and a rotating support 8. A rotating seat 6 is fixed on the rotating support 8, and a rotating frame 5 is rotatably connected to the rotating seat 6. A connecting frame 4 is rotatably connected to one end of the rotating frame 5. Both the rotating support 8 and the fixed support 1 are fixed to the stage of the inverted microscope by bolts. A mounting frame 2 is fixed on the fixed support 1, and a tilt adjustment mechanism 3 is provided on the mounting frame 2. The tilt adjustment mechanism 3 includes a fine-tuning gearbox 31 fixed to the top of the mounting frame 2. A fine-tuning knob 32 is provided at the input end of the fine-tuning gearbox 31, and a docking frame 33 is fixed at the output end of the fine-tuning gearbox 31. (A worm gear drive is provided in the fine-tuning gearbox 31.) The rotating gear set and the fine-tuning reduction gearbox 31 are existing technologies and will not be described in detail here. The rotation angle of the docking frame 33 can be adjusted by rotating the fine-tuning knob 32. The docking frame 33 is slidably connected to the alignment ring 34 through a slide rail on the outer wall. The alignment ring 34 is rotatably connected to the push frame 35, which is fixedly connected to the output end of the docking electric cylinder 36. The docking electric cylinder 36 is fixed on the fine-tuning reduction gearbox 31. Under the output action of the docking electric cylinder 36, the alignment ring 34 abuts against the driven ring 37. The driven ring 37 has symmetrically opened alignment grooves 371, and the alignment ring 34 has symmetrically arranged alignment protrusions 372 that abut against the alignment grooves 371. The alignment protrusions 372 are completely embedded in the alignment grooves 371. After step 1, the position of the driven ring 37 is restricted by the alignment ring 34. The driven ring 37 is fixed on the connecting frame 4. A quick reset mechanism 38 is provided on the driven ring 37. When using the same type of cell culture flask for batch testing, the previous observation tilt angle can be quickly reset using the quick reset mechanism 38 after the cell flask is fixed. The quick reset mechanism 38 includes a driven sleeve 381 fixed in the driven ring 37. A first magnet 382 is nested in a groove symmetrically opened on the side wall of the driven sleeve 381. An active sleeve 383 is fixed on the connecting frame 33. A second magnet 384 is nested in a groove symmetrically opened on the side wall of the active sleeve 383. The magnetic force between the second magnet 384 and the first magnet 382 will drive the driven sleeve. 381 Rotation, the connecting frame 4 is fixed with a centering clamping mechanism 7, the centering clamping mechanism 7 is used to clamp the culture flask containing cell culture medium, the centering clamping mechanism 7 includes a side support 71 fixed to the connecting frame 4 by bolts, the side support 71 is provided with a bottle mouth clamping mechanism 72, the bottle mouth clamping mechanism 72 is used to clamp the bottle mouth of the T-shaped cell culture flask for cell culture, the bottom of the connecting frame 4 is provided with a driving mechanism 73, the driving mechanism 73 is connected to a pressing centering mechanism 74, the driving mechanism 73 can drive the pressing centering mechanism 74 to press down the top slope of the T-shaped cell culture flask, thereby making the cell flask parallel to the side support 71, ensuring that the tilt direction of the bottom observation surface of the cell flask is consistent each time it is clamped and observed;

[0033] When using the adapter, the fixed support 1 and the rotating support 8 need to be fixed to the side wall of the stage of the inverted microscope with bolts, and it should be ensured that the fixed support 1 and the rotating support 8 are installed at the same height. When performing cell observation, first rotate the rotating frame 5 and the connecting frame 4 around the rotating base 6 to move them out of the space above the stage. Then, the bottle mouth clamping mechanism 72 in the centering clamping mechanism 7 clamps the bottle mouth of the T-shaped cell culture flask used for observation. At the same time, the downward pressing centering mechanism 74 presses down the top slope of the culture flask to make the cell flask parallel to the side support 71. Then, rotate the rotating frame 5 in the opposite direction to make the docking frame 33 parallel and aligned with the alignment ring 34 and lock them. Positioning the rotating frame 5 on the rotating support 8, the driven sleeve 381, under the magnetic attraction of the second magnet 384 and the first magnet 382, ​​will drive the driven ring 37 and the connecting frame 4 to rotate, aligning the alignment groove 371 and the alignment protrusion 372. Then, the output end of the docking electric cylinder 36 extends, pushing the alignment ring 34 against the driven ring 37 via the pusher 35. After complete contact, the alignment groove 371 and the alignment protrusion 372 nest together, allowing the alignment ring 34 to rotate synchronously with the driven ring 37. Then, rotating the fine-tuning knob 32, via the fine-tuning reduction gearbox 31, will drive the docking frame 33, the alignment ring 34, and the connecting frame 4 to rotate synchronously, making... The cell flask is tilted until a clear image of the cells is observed through the eyepiece of the inverted microscope. Then, while maintaining the tilt angle, the spatial position of the stage is adjusted to observe and record the cell condition in the flask. When observing a batch of the same type of cell flask, the tilt angle is adjusted using the fine-tuning knob 32 for the first observation. Subsequent observations can be quickly reset using the quick-reset mechanism 38. Specifically, during repeated observations, without rotating the fine-tuning knob 32, the rotation angle of the docking frame 33 is maintained via the fine-tuning gearbox 31. Then, the rotating frame 5 and connecting frame 4 are rotated around the rotating base 6 and moved out of the space above the stage. The T-shaped cell culture flask for the next observation is re-clamped by the centering clamping mechanism 7. Then, the rotating frame 5 is reset. Under the magnetic attraction of the second magnet 384 and the first magnet 382, ​​the driven sleeve 381 will drive the driven ring 37 and the connecting frame 4 to rotate and reset quickly, so that the connecting frame 4 initially returns to its previous rotation position. At this time, the output end of the docking electric cylinder 36 extends and pushes the alignment ring 34 against the driven ring 37 through the push frame 35. After complete contact, the alignment groove 371 and the alignment protrusion 372 nest with each other, preventing the connecting frame 4 and the centering clamping mechanism 7 from swinging, while making the cell flask completely reset, ensuring the processing efficiency of repeated observations.

[0034] The bottle mouth clamping mechanism 72 includes a side guide rail disposed on one side of the side support 71. An upper clamping block 721 is fixed to a slider symmetrically slidably connected to the side guide rail. A limit post 722 is slidably connected in a through groove on the upper clamping block 721. The limit post 722 is symmetrically fixed to the top of the clamping disc 723. During the rotation of the clamping disc 723, the limit post 722 can drive the upper clamping blocks 721 on both sides to move closer or further away synchronously, thereby achieving the clamping and unlocking effect. The drive mechanism 73 includes a drive motor 731 fixed to the bottom of the connecting frame 4. A square drive rod 732 is fixed to the output end of the drive motor 731. The drive rod 732 is slidably connected to a square groove at the bottom of the drive sleeve 733. A spiral guide protrusion 734 is provided on the upper side wall of the drive sleeve 733. The drive sleeve 733 is slidably connected to a fixed sleeve 735. A guide slide 736 is provided on the inner wall of the fixed sleeve 735. The guide slide 736 is slidably connected to the guide protrusion 734. The top of component 5 is rotatably connected to the connecting frame 4, and the clamping plate 723 is fixedly sleeved on the fixed sleeve 735. The downward pressing centering mechanism 74 includes an upper support 741 rotatably connected to the driving sleeve 733, a lower support 742 slidably connected to the bottom of the upper support 741, and the lower support 742 is fixed to the connecting frame 4. A center rod 743 is provided on the connecting frame 4, and the center rod 743 is slidably connected to the upper support 741. A support spring 744 is provided between the upper support 741 and the connecting frame 4. A support spring 744 is sleeved on the central rod 743, and the support spring 744 is in the closed space between the upper support 741 and the lower support 742. A lifting frame 745 is slidably connected to the connecting frame 4. The lifting frame 745 is fixed to the top of the upper support 741. V-shaped frames 746 are rotatably connected to both sides of the lifting frame 745. An upper cover plate 748 is fixed on the V-shaped frame 746, and pressure rollers 747 are symmetrically arranged on the V-shaped frame 746. The pressure rollers 747 are located inside the upper cover plate 748.

[0035] When clamping a T-shaped cell culture flask, first place the mouth of the flask between the two upper clamping blocks 721. Then, drive motor 731 drives drive rod 732 to rotate. Drive rod 732 engages with the square groove at the bottom of drive sleeve 733, causing drive sleeve 733 to rotate synchronously. At this time, under the elastic support of support spring 744, lifting frame 745, upper support 741, and drive sleeve 733 are all in a high position. Drive sleeve 733 transmits rotational torque to fixed sleeve 735 through guide slide 736 and guide protrusion 734. Fixed sleeve 735 drives clamping disk 723 to rotate, and through limit post 722, drives the two... The upper clamping block 721 on the side moves closer to clamp the mouth of the cell culture flask. After being fully clamped, the mouth of the cell culture flask restricts the upper clamping block 721 from moving closer. The position of the clamping plate 723 and the fixing sleeve 735 is restricted. As the drive rod 732 continues to rotate, the drive sleeve 733 will rotate and move down under the action of the guide slide 736. During the downward movement, the upper support 741 and the lifting frame 745 are pulled down. The downward movement of the lifting frame 745 will compress the support spring 744 and drive the V-shaped frame 746 to move down until the pressure rollers 747 on the V-shaped frame 746 are pressed tightly on the top inclined surface of the T-shaped cell culture flask, completing the clamping process.

[0036] It should be noted that the tilting rotary adapter for the cell culture flask used in this inverted microscope requires the following steps: First, the fixed support 1 and the rotating support 8 are fixed to the side wall of the stage of the inverted microscope with bolts, ensuring that their installation heights are consistent. Before observation, the rotating frame 5 and the connecting frame 4 are rotated out of the area above the stage around the rotating base 6. The T-shaped cell culture flask is then clamped using the centering clamping mechanism 7. During this process: the mouth of the T-shaped cell culture flask is placed between the two upper clamping blocks 721, and the drive motor 731 is started. The drive motor 731 drives the drive sleeve 733 to rotate via the drive rod 732. Under the support of the support spring 744, the drive... The moving sleeve 733 first drives the fixed sleeve 735 and the clamping plate 723 to rotate through the guide protrusion 734 and the guide slide 736. The clamping plate 723 drives the upper clamping blocks 721 on both sides to move closer simultaneously through the limiting post 722, so as to achieve the centering clamping of the bottle mouth. After the bottle mouth is clamped in place, the clamping plate 723 and the fixed sleeve 735 are restricted from rotating. The driving sleeve 733 continues to rotate and move downward under the action of the guide slide 736, pulling the upper support 741, the lifting frame 745 and the V-shaped frame 746 to move downward, so that the pressure roller 747 presses against the top slope of the T-shaped cell culture bottle, so that the cell bottle and the side support 71 remain parallel, and the clamping and centering are completed.

[0037] After clamping, rotate the rotating frame 5 back so that the connecting frame 4 is opposite to the docking frame 33. At this time, the second magnet 384 in the active sleeve 383 and the first magnet 382 in the driven sleeve 381 generate a magnetic attraction, which drives the driven sleeve 381, the driven ring 37 and the connecting frame 4 to rotate circumferentially, realizing magnetic coarse positioning, so that the alignment groove 371 and the alignment protrusion 372 are roughly aligned. Then the docking electric cylinder 36 extends and drives the alignment ring 34 to move closer to the driven ring 37 through the push frame 35, so that the alignment protrusion 372 is embedded in the alignment groove 371, completing the circumferential fine positioning and restricting the rotational freedom of the driven ring 37 and the connecting frame 4.

[0038] During the initial debugging, rotating the fine-tuning knob 32 causes the fine-tuning gearbox 31 to rotate the docking frame 33, alignment ring 34, driven ring 37, and connecting frame 4 as a whole, adjusting the tilt angle of the T-shaped cell culture flask until a clear cell observation image is obtained in the eyepiece, thus completing the angle calibration. When observing cell flasks of the same specification in batches, keep the fine-tuning knob 32 stationary to lock the angle of the docking frame 33. After changing the cell flask and repeating the clamping and resetting of the rotating frame 5, the driven ring 37 quickly resets circumferentially under the magnetic attraction of the first magnet 382 and the second magnet 384. Then, the docking electric cylinder 36 pushes the alignment ring 34 to complete the mechanical fine positioning, which can quickly reproduce the previous tilt angle without further fine-tuning. After the angle remains unchanged, the bottom observation surface of the cell flask can be fully observed and recorded by moving the microscope stage.

[0039] The embodiments described above are merely examples of several implementations of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention.

Claims

1. A tilting rotation adapter for cell culture flasks used in inverted microscopes, comprising a fixed support (1), characterized in that: A mounting frame (2) is provided on the fixed support (1), and a tilt adjustment mechanism (3) is provided on the mounting frame (2). The tilt adjustment mechanism (3) includes a fine-tuning gearbox (31) fixed on the top of the mounting frame (2). A fine-tuning knob (32) is provided at the input end of the fine-tuning gearbox (31), and a docking frame (33) is fixed at the output end of the fine-tuning gearbox (31). An alignment ring (34) is slidably connected on the docking frame (33), and a push frame (35) is rotatably connected on the alignment ring (34). The push frame (35) is fixedly connected to the output end of the docking electric cylinder (36), and the docking electric cylinder (36) is fixed on the... On the fine-tuning gearbox (31), the alignment ring (34) abuts against the driven ring (37), the driven ring (37) is fixed on the connecting frame (4), the connecting frame (4) is rotatably connected to the rotating frame (5), the rotating frame (5) is rotatably connected to the rotating seat (6), the connecting frame (4) is fixed with the centering clamping mechanism (7), the centering clamping mechanism (7) includes a side support (71) fixed on the connecting frame (4), the side support (71) is provided with a bottle mouth clamping mechanism (72), the bottom of the connecting frame (4) is provided with a driving mechanism (73), the driving mechanism (73) is connected to the pressing centering mechanism (74).

2. The tilting rotary adapter for cell culture flasks for inverted microscopes according to claim 1, characterized in that: The driven ring (37) is symmetrically provided with alignment grooves (371), and the alignment ring (34) is symmetrically provided with alignment protrusions (372) that abut against the alignment grooves (371).

3. The tilting rotary adapter for cell culture flasks for inverted microscopes according to claim 1, characterized in that: The driven ring (37) is provided with a quick reset mechanism (38), which includes a driven sleeve (381) fixed in the driven ring (37), and a first magnet (382) is nested in a groove symmetrically opened on the side wall of the driven sleeve (381).

4. The tilting rotary adapter for cell culture flasks for inverted microscopes according to claim 3, characterized in that: An active sleeve (383) is fixed on the docking frame (33), and a second magnet (384) that attracts the first magnet (382) is nested in a groove symmetrically opened on the side wall of the active sleeve (383).

5. The tilting rotary adapter for cell culture flasks for inverted microscopes according to claim 1, characterized in that: The bottle mouth clamping mechanism (72) includes an upper clamping block (721) symmetrically slidably connected to the side support (71), and a limiting post (722) slidably connected in a through groove on the upper clamping block (721), and the limiting post (722) is symmetrically fixed to the top of the clamping plate (723).

6. The tilting rotary adapter for cell culture flasks for inverted microscopes according to claim 5, characterized in that: The drive mechanism (73) includes a drive motor (731) fixed to the bottom of the connecting frame (4), and a square drive rod (732) fixed to the output end of the drive motor (731). The drive rod (732) is slidably connected in a square groove at the bottom of the drive sleeve (733).

7. The tilting rotary adapter for cell culture flasks for inverted microscopes according to claim 6, characterized in that: The drive sleeve (733) has a guide protrusion (734) on its side wall. The drive sleeve (733) is slidably connected in the fixed sleeve (735). The guide slide (736) opened in the inner wall of the fixed sleeve (735) is slidably connected to the guide protrusion (734). The fixed sleeve (735) is rotatably connected to the connecting frame (4). The clamping plate (723) is fixedly sleeved on the fixed sleeve (735).

8. The tilting rotary adapter for cell culture flasks for inverted microscopes according to claim 6, characterized in that: The lowering centering mechanism (74) includes an upper support (741) rotatably connected to the drive sleeve (733), a lower support (742) fixed to the connecting frame (4) slidably connected to the bottom of the upper support (741), a center rod (743) slidably connected to the upper support (741) on the connecting frame (4), and a support spring (744) between the upper support (741) and the connecting frame (4), the support spring (744) being sleeved on the center rod (743).

9. The tilting rotary adapter for cell culture flasks for inverted microscopes according to claim 8, characterized in that: A lifting frame (745) is slidably connected to the connecting frame (4). The lifting frame (745) is fixed on the top of the upper support (741). V-shaped frames (746) are rotatably connected to both sides of the lifting frame (745). An upper cover plate (748) is fixed on the V-shaped frame (746), and pressure rollers (747) located inside the upper cover plate (748) are symmetrically fixed on the V-shaped frame (746).

10. The tilting rotary adapter for cell culture flasks for inverted microscopes according to claim 1, characterized in that: The rotating seat (6) is fixed on the rotating support (8), and both the rotating support (8) and the fixed support (1) are fixed on the microscope stage.

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