A small portable liquid nitrogen transfer device for embryonic reproductive laboratory

Abstract

This invention discloses a small, portable liquid nitrogen transport device for embryology laboratories, relating to the field of liquid nitrogen transport devices. It includes a transport base comprising a movable base plate and a buffer platform. The buffer platform is fixedly mounted on the upper surface of the movable base plate, and a pump assembly is fixedly mounted on the buffer platform. A liquid storage tank assembly connected to the pump assembly is also fixedly mounted on the movable base plate, and a multi-functional housing is fastened and fixed to the movable base plate. The main body of this invention uses a movable base plate composed of an integrated tray and casters. The casters allow the device to move flexibly within the limited space of a laboratory, facilitating the transport of liquid nitrogen to various required locations. This invention utilizes a transparent measuring container with volume markings on it, which, in conjunction with a drive assembly, precisely controls the movement of the lateral slide, enabling accurate addition of a fixed amount of liquid nitrogen to the liquid nitrogen tank storing embryos.

Description

Technical Field

[0001] This invention relates to the field of liquid nitrogen transport device technology, specifically a small portable liquid nitrogen transport device for use in embryo reproductive laboratories. Background Technology

[0002] In embryology laboratories, the use of liquid nitrogen is crucial for the cryopreservation of embryos. A suitable liquid nitrogen environment can provide stable low-temperature conditions for embryos, maximizing their viability and developmental potential.

[0003] Traditional liquid nitrogen transport equipment is often bulky and lacks flexible mobility. Within the limited space of a laboratory, these devices are difficult to move freely and cannot easily transport liquid nitrogen to various required locations.

[0004] In the liquid nitrogen filling process, existing methods struggle to achieve precise quantitative control. Many laboratories still rely on manual experience to determine the amount of liquid nitrogen to add, which can easily lead to waste or insufficient replenishment. On one hand, adding too much liquid nitrogen not only wastes resources and increases laboratory operating costs but may also put unnecessary stress on equipment such as liquid nitrogen tanks, shortening their lifespan. On the other hand, insufficient liquid nitrogen replenishment cannot provide a adequate low-temperature environment for embryos, potentially affecting the cryopreservation effect and reducing embryo survival rates. Moreover, existing filling equipment is complex to operate; the process of replenishing liquid nitrogen from the main tank to the storage tank is cumbersome, requiring multiple steps and additional tools, significantly reducing work efficiency. Summary of the Invention

[0005] To achieve flexible and safe transport of liquid nitrogen, this application provides a small portable liquid nitrogen transport device for embryo reproductive laboratories.

[0006] This invention is implemented as follows: A small portable liquid nitrogen transport device for embryology laboratories includes a transport base, which comprises a movable base plate and a buffer platform. The buffer platform is fixedly installed on the upper surface of the movable base plate, and a pump assembly is fixedly installed on the buffer platform. A liquid storage tank assembly connected to the pump assembly is also fixedly installed on the movable base plate, and a multi-functional housing is fastened and fixedly mounted on the movable base plate. A quantitative liquid dispensing tank assembly is slidably installed on the multi-functional housing, and a drive assembly for driving the quantitative liquid dispensing tank assembly to extend and retract is also installed on the multi-functional housing.

[0007] By adopting the above technical solution, the transfer base achieves overall integration and portability of the device through the fixed installation of the movable seat plate and buffer platform. The connection between the pump liquid assembly and the liquid storage tank group ensures the closed-loop circulation of liquid nitrogen. The multi-functional housing provides protection and sliding space through its fastening and fixing. The sliding installation of the quantitative liquid addition tank group, combined with the telescopic drive of the drive assembly (such as a stepper motor to precisely control the stroke), realizes the automated quantitative process of liquid nitrogen from storage to filling, avoiding evaporation and spillage caused by manual pouring, and improving the accuracy and safety of laboratory operations.

[0008] Furthermore, the movable seat includes an integrated tray and casters. The casters are installed on the lower end face of the integrated tray near the edge, and the integrated tray has positioning holes for mounting the buffer platform.

[0009] By adopting the above technical solution, the movable seat plate uses an integrated tray and casters. The casters are installed on the lower end face of the integrated tray near the edge, giving the device good mobility. The positioning holes on the integrated tray provide accurate positioning for the buffer platform installation, ensuring the stability and accuracy of the installation, thereby ensuring the stability of the entire device structure and facilitating the normal operation of each component during movement.

[0010] Furthermore, the buffer platform includes a base and a clamping seat. The clamping seat is slidably mounted on the upper end face of the base, and a long screw connected to the base is fixedly mounted on the clamping seat. A locking nut that matches the long screw is mounted on the long screw. A plug-in screw corresponding to the positioning hole is fixedly mounted on the lower end face of the base, and a buffer ring is also fixedly mounted on the lower end face of the base.

[0011] By adopting the above technical solution, the base and clamping seat structure of the buffer platform, through the cooperation of long screws and locking nuts, allows for flexible adjustment of the clamping seat position, facilitating the installation and fixation of the pumping components. The insertion screw on the lower end face of the base, engaging with the positioning hole, further enhances the stability of the connection between the buffer platform and the moving base plate. The buffer ring effectively dampens vibrations generated during device movement, reducing the impact of vibrations on components such as the pumping components, extending the service life of components, and improving the reliability of the device.

[0012] Furthermore, the pump assembly includes a peristaltic pump housing, a U-shaped hose, and a pump head that mates with the U-shaped hose. The peristaltic pump housing is fixedly installed between the base and the clamping seat. The U-shaped hose is fixedly installed in the peristaltic pump housing, with both ends of the U-shaped hose extending out of the peristaltic pump housing. The pump head is rotatably installed in the peristaltic pump housing, and an internal pump motor for driving the pump head to rotate is fixedly installed in the peristaltic pump housing. The two ends of the U-shaped hose are respectively sealed and connected to a first connecting pipe and a second connecting pipe. The other end of the first connecting pipe is sealed and connected to a liquid storage tank group. The other end of the second connecting pipe is equipped with a three-way valve. The inlet of the three-way valve is sealed and connected to the second connecting pipe, and the first outlet of the three-way valve is sealed and connected to a metering liquid addition tank group. An adsorption hose is sealed and connected to the second outlet of the three-way valve, and an adsorption head is sealed and connected to the outer end of the adsorption hose.

[0013] By adopting the above technical solution, the combined design of the peristaltic pump casing, U-shaped hose, and pump head of the pump assembly, driven by an internal motor, enables the transfer of liquid nitrogen. The U-shaped hose connects to a storage tank and a three-way valve at both ends. With the three-way valve offering different outlet options, it can replenish liquid nitrogen from the main tank to the storage tank and transfer liquid nitrogen from the storage tank to a metered filling tank. The adsorption hose and adsorption head facilitate the extraction of liquid nitrogen from the main tank. When not in use, the adsorption head can be stored in a housing, preventing liquid nitrogen leakage and damage to the adsorption head.

[0014] Furthermore, the liquid storage tank assembly includes a tank shell and auxiliary support blocks. The auxiliary support blocks are evenly fixed on the lower end face of the tank shell and fixedly installed on the upper end face of the integrated support plate. An auxiliary valve is also installed on the upper end face of the tank shell.

[0015] By adopting the above technical solution, the tank shell and auxiliary support structure of the liquid storage tank group are designed so that the auxiliary support blocks are evenly fixed on the lower end face of the tank shell and installed on the integrated support plate, ensuring the stability of the tank shell. The auxiliary valve on the upper end face of the tank shell can easily control the inlet and outlet of liquid nitrogen and the storage pressure, ensuring the safety and stability of liquid nitrogen storage.

[0016] Furthermore, the multifunctional housing includes a snap-on housing and a sleeve housing. The snap-on housing is sleeved and fixed on the integrated tray, and the sleeve housing is fixedly installed on one side of the snap-on housing and sleeved and fixed on the outer side of the barrel shell. A storage shell is also fixedly installed on one side of the snap-on housing. A flip-open top cover is rotatably installed on the upper end of the storage shell. An operating frame is installed on the outer side of the sleeve housing, and the operating frame is rotatably connected to the sleeve housing.

[0017] By adopting the above technical solution, the design of the snap-fit ​​part and the sleeve part of the multi-functional housing, with the snap-fit ​​part sleeved and fixed on the integrated tray and the sleeve part sleeved and fixed on the outer surface of the barrel shell, enhances the compactness and stability of the overall structure of the device. The storage shell and the flip-open top cover facilitate the storage of the adsorption head, and the setting of the operating frame facilitates the movement and operation of the device by a single operator, improving the ease of use of the device.

[0018] Furthermore, the quantitative liquid addition tank assembly includes a horizontal slide, a transparent measuring cylinder, and a large-diameter dispensing nozzle. The horizontal slide is slidably mounted on the snap-fit ​​housing. The transparent measuring cylinder is fixedly mounted on the upper end face of the horizontal slide, and a volume scale is provided on the outer side of the transparent measuring cylinder. The large-diameter dispensing nozzle is fixedly mounted at the center of the lower end face of the transparent measuring cylinder, and a control valve is installed in the large-diameter dispensing nozzle. A top cover is fixedly mounted on the upper end face of the transparent measuring cylinder. A flip cover is mounted on the upper end face of the top cover, and a functional shell is integrally formed on the lower end face of the top cover. A sampling tube extending into the transparent measuring cylinder is sealed and connected to the lower end face of the functional shell. A one-way valve is installed at the head of the sampling tube, and a manual sampling component is provided in the functional shell.

[0019] By adopting the above technical solution, the structure of the quantitative liquid addition tank assembly—including the horizontal slide, transparent measuring cylinder, and large-diameter dispensing nozzle—allows the horizontal slide to slide on the snap-fit ​​section, facilitating adjustment of the liquid addition position. The volume scale on the outer side of the transparent measuring cylinder allows operators to accurately control the liquid addition volume. The large-diameter dispensing nozzle and control valve enable rapid and accurate addition of liquid nitrogen to the required liquid nitrogen tank. The combination of the top seal, flip-top cover, functional housing, sampling tube, one-way dispensing valve, and manual sampling components provides more operational methods and functional options for liquid nitrogen sampling and filling.

[0020] Furthermore, the drive assembly includes an external motor and a transverse screw. The external motor is fixedly mounted on the outer side of the snap-fit ​​housing. One end of the transverse screw is fixed to the output shaft of the external motor, and the inner end of the transverse slide is provided with a threaded hole that mates with the transverse screw.

[0021] By adopting the above technical solution, the external motor of the drive component works in conjunction with the transverse screw. The external motor drives the transverse screw to rotate, and the transverse slide moves using the threaded hole inside the slide block, thereby precisely controlling the extension and retraction of the metering liquid dispensing tank assembly. This design enables precise control of metering liquid dispensing, improving the accuracy and efficiency of dispensing.

[0022] Furthermore, the manual sampling assembly includes an extraction tube, an auxiliary chuck, and a piston rod. The lower end face of the extraction tube is integrally formed with a connector head for connecting to the sampling tube. The auxiliary chuck is fixedly installed at the head of the extraction tube. The piston rod is installed in the extraction tube and is in a sealed sliding connection with the extraction tube.

[0023] By adopting the above technical solution, the structure of the extraction tube, auxiliary chuck, and piston rod of the first manual sampling component, through the sealed sliding of the piston rod in the extraction tube and the connection between the insertion tube head and the sampling tube, can realize the function of manually extracting liquid nitrogen. This structure is simple and easy to operate.

[0024] Furthermore, the manual sampling assembly includes a sampling scoop and a pull rod. The upper surface of the sampling scoop has a sampling groove, and a liquid inlet check valve is installed on the inner side of the bottom surface of the sampling scoop. The pull rod is vertically fixed to the upper surface of the sampling scoop, and a sealing ring that is sealed to the functional housing is provided on the outer surface of the sampling scoop.

[0025] By adopting the above technical solution, the second manual sampling component, with its sampling scoop, pull rod, liquid inlet check valve, and sealing ring, achieves manual sampling by pulling the sampling scoop with the pull rod and controlling the entry of liquid nitrogen using the liquid inlet check valve. The sealing ring ensures airtightness during the sampling process, preventing liquid nitrogen leakage. This structure is suitable for different sampling scenarios, increasing the practicality and flexibility of the device.

[0026] Compared with the prior art, the beneficial effects of the present invention are: The main body of this invention uses a movable seat composed of an integrated tray and casters. The casters allow the device to move flexibly within the limited space of a laboratory, facilitating the transfer of liquid nitrogen to various required locations. Simultaneously, the use of quiet casters reduces noise interference during movement, ensuring no disruption to the normal working environment of the laboratory.

[0027] This invention utilizes a transparent measuring cylinder with volume markings on its quantitative liquid addition tank assembly. Combined with a drive assembly that precisely controls the movement of the lateral slide, it accurately adds a measured amount of liquid nitrogen to the liquid nitrogen tank storing embryos. This avoids waste and insufficient replenishment of liquid nitrogen, ensuring the effectiveness of embryo cryopreservation. Through the cooperation of the pump assembly and the three-way valve, when the second outlet of the three-way valve is opened, liquid nitrogen can be easily replenished from the main tank to the liquid storage tank assembly. The operation is simple and quick, improving work efficiency.

[0028] The present invention also has the advantage of functional versatility. The device is equipped with two structures for the manual sampling component, and users can choose the appropriate sampling method according to actual needs, which increases the practicality and flexibility of the device. Attached Figure Description

[0029] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.

[0030] Figure 1 This is a perspective view of the overall structure in an embodiment of the present invention; Figure 2 yes Figure 1 A front view of the device shown; Figure 3 This is a perspective view of the transfer base, pump assembly, and liquid storage tank assembly in an embodiment of the present invention. Figure 4 yes Figure 3 A front view of the device shown; Figure 5 yes Figure 3 A secondary view of the device shown; Figure 6 This is a perspective view of the buffer platform and liquid storage tank assembly in an embodiment of the present invention. Figure 7 yes Figure 6 A front view of the device shown; Figure 8 This is a perspective view of the movable seat plate and the pump assembly in cooperation in an embodiment of the present invention; Figure 9 yes Figure 8 A front view of the device shown; Figure 10 yes Figure 8 Side view of the device shown; Figure 11 This is a perspective view of the multifunctional housing and quantitative liquid dispensing tank assembly in an embodiment of the present invention. Figure 12 yes Figure 11 A bottom view of the device shown; Figure 13 This is a perspective view of the flip-top cover in an embodiment of the present invention; Figure 14 yes Figure 13 A front view of the device shown; Figure 15 This is a perspective view of the manual sampling component in Embodiment 1 of the present invention; Figure 16 yes Figure 15 A front view of the device shown; Figure 17 This is a perspective view of the manual sampling component in Embodiment 2 of the present invention; Figure 18 yes Figure 17 Front view of the device shown.

[0031] In the diagram: 1. Transfer base; 11. Movable seat plate; 111. Integrated tray; 112. Casters; 113. Positioning hole; 12. Buffer platform; 121. Base; 122. Clamping seat; 123. Long screw; 124. Buffer ring; 125. Insert screw; 2. Pump assembly; 21. Peristaltic pump housing; 22. U-shaped hose; 221. First connecting pipe; 222. Second connecting pipe; 23. Pump head; 24. Three-way valve; 25. Adsorption hose; 251. Adsorption head; 3. Liquid storage tank assembly; 31. Tank shell; 311. Auxiliary valve; 32. Auxiliary support block; 4. Multifunctional housing; 41. Casing unit; 411. Storage casing; 412. Top cover; 42. Covering unit; 421. Operating frame; 5. Quantitative liquid addition tank assembly; 51. Horizontal slide; 52. Transparent measuring tank; 520. Flip-top cover; 521. Top sealing cover; 522. Functional casing; 523. Sampling tube; 53. Large-diameter liquid outlet; 6. Drive assembly; 61. External motor; 62. Horizontal screw; 8. Manual sampling assembly; 801. Sampling scoop; 802. Pull rod; 803. Liquid inlet check valve; 804. Sealing ring; 81. Extraction tube; 811. Connecting tube head; 82. Auxiliary chuck; 83. Piston rod. Detailed Implementation

[0032] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention.

[0033] Example 1 Reference Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5A small portable liquid nitrogen transport device for embryo reproduction laboratory includes a transport base 1, which includes a movable base plate 11 and a buffer platform 12. The buffer platform 12 is fixedly installed on the upper surface of the movable base plate 11. A pump assembly 2 is fixedly installed on the buffer platform 12. A liquid storage tank group 3 connected to the pump assembly 2 is also fixedly installed on the movable base plate 11. A multi-functional housing 4 is fastened and fixed on the movable base plate 11. A quantitative liquid dispensing tank group 5 is slidably installed on the multi-functional housing 4. A drive assembly 6 for driving the quantitative liquid dispensing tank group 5 to extend and retract is also installed on the multi-functional housing 4. The transfer base 1 achieves overall integration and portability of the device through the fixed installation of the movable seat plate 11 and the buffer platform 12. The connection between the pump assembly 2 and the storage tank group 3 ensures the closed-loop circulation of liquid nitrogen. The multi-functional housing 4 provides protection and sliding space through its fastening and fixing. The sliding installation of the quantitative liquid addition tank group 5, combined with the telescopic drive of the drive assembly 6 (such as a stepper motor to precisely control the stroke), realizes the automated quantitative process of liquid nitrogen from storage to filling, avoiding evaporation and spillage caused by manual pouring, and improving the accuracy and safety of laboratory operations.

[0034] Reference Figure 3 , Figure 4 , Figure 6 and Figure 10The movable seat 11 includes an integrated tray 111 and casters 112. The casters 112 are installed near the edge of the lower end face of the integrated tray 111, and the integrated tray 111 has positioning holes 113 for mounting the buffer platform 12. The movable seat 11's design of the integrated tray 111 and casters 112, with the casters 112 installed near the edge of the lower end face of the integrated tray 111, provides excellent mobility. The positioning holes 113 on the integrated tray 111 provide accurate positioning for the buffer platform 12, ensuring installation stability and accuracy, thereby ensuring the overall structural stability of the device and facilitating the normal operation of all components during movement. The buffer platform 12 includes a base 121 and a clamping seat 122. The clamping seat 122 is slidably mounted on the upper surface of the base 121, and a long screw 123 connected to the base 121 is fixedly mounted on the clamping seat 122. A locking nut is installed on the long screw 123. A plug-in screw 125 corresponding to the positioning hole 113 is fixedly mounted on the lower surface of the base 121, and a buffer ring 124 is also fixedly mounted on the lower surface of the base 121. The structure of the base 121 and clamping seat 122 of the buffer platform 12 allows for flexible adjustment of the position of the clamping seat 122 through the cooperation of the long screw 123 and the locking nut, facilitating the installation and fixation of the pump assembly 2. The plug-in screw 125 on the lower surface of the base 121 cooperates with the positioning hole 113, further enhancing the stability of the connection between the buffer platform 12 and the movable base plate 11. The buffer ring 124 can effectively buffer the vibration generated during the movement of the device, reduce the impact of vibration on components such as the pump liquid assembly 2, extend the service life of the components, and improve the reliability of the device.

[0035] Reference Figure 3 , Figure 7 , Figure 8 and Figure 9The pump assembly 2 includes a peristaltic pump housing 21, a U-shaped hose 22, and a pump head 23 that mates with the U-shaped hose 22. The peristaltic pump housing 21 is fixedly installed between the base 121 and the clamping seat 122. The U-shaped hose 22 is fixedly installed in the peristaltic pump housing 21, with both ends of the U-shaped hose 22 extending out of the peristaltic pump housing 21. The pump head 23 is rotatably installed in the peristaltic pump housing 21, and an internal pump motor for driving the pump head 23 to rotate is fixedly installed in the peristaltic pump housing 21. The two ends of the U-shaped hose 22 are respectively sealed... The system is sealed with a first connecting pipe 221 and a second connecting pipe 222. The other end of the first connecting pipe 221 is sealed to the liquid storage tank group 3, and the other end of the second connecting pipe 222 is equipped with a three-way valve 24. The inlet of the three-way valve 24 is sealed to the second connecting pipe 222, and the first outlet of the three-way valve 24 is sealed to the quantitative liquid addition tank group 5. An adsorption hose 25 is sealed to the second outlet of the three-way valve 24, and an adsorption head 251 is sealed to the outer end of the adsorption hose 25. The combination design of the peristaltic pump housing 21, the U-shaped hose 22, and the pump head 23 of the pump assembly 2 enables the pump head 23 to rotate through the internal motor, thereby realizing the function of liquid nitrogen delivery. The two ends of the U-shaped hose 22 are respectively connected to the liquid storage tank group 3 and the three-way valve 24. With the different outlet selections of the three-way valve 24, the system can replenish liquid nitrogen from the main tank to the liquid storage tank group 3 and deliver liquid nitrogen from the liquid storage tank group 3 to the quantitative liquid addition tank group 5. The adsorption hose 25 and adsorption head 251 are designed to facilitate the extraction of liquid nitrogen from the main tank. When not in use, the adsorption head 251 can be placed in the storage shell 411, which avoids liquid nitrogen leakage and damage to the adsorption head 251.

[0036] Reference Figure 5 and Figure 6 The liquid storage tank assembly 3 includes a tank shell 31 and auxiliary support blocks 32. The auxiliary support blocks 32 are evenly fixed to the lower end face of the tank shell 31 and are also fixedly installed on the upper end face of the integrated support plate 111. An auxiliary valve 311 is also installed on the upper end face of the tank shell 31. The structure of the tank shell 31 and auxiliary support blocks 32 of the liquid storage tank assembly 3, with the auxiliary support blocks 32 evenly fixed to the lower end face of the tank shell 31 and installed on the integrated support plate 111, ensures the stability of the tank shell 31. The auxiliary valve 311 on the upper end face of the tank shell 31 can conveniently control the inlet and outlet of liquid nitrogen and the storage pressure, ensuring the safety and stability of liquid nitrogen storage.

[0037] Reference Figure 11The multi-functional housing 4 includes a snap-fit ​​portion 41 and a sleeve portion 42. The snap-fit ​​portion 41 is fitted and fixed onto the integrated tray 111, and the sleeve portion 42 is fixedly installed on one side of the snap-fit ​​portion 41 and fitted and fixed onto the outer surface of the barrel shell 31. A storage shell 411 is also fixedly installed on one side of the snap-fit ​​portion 41. A flip-open top cover 412 is rotatably installed on the upper end of the storage shell 411. An operating frame 421 is installed on the outer surface of the sleeve portion 42 and is rotatably connected to the sleeve portion 42. The design of the snap-fit ​​portion 41 and the sleeve portion 42 of the multi-functional housing 4, with the snap-fit ​​portion 41 fitted and fixed onto the integrated tray 111 and the sleeve portion 42 fitted and fixed onto the outer surface of the barrel shell 31, enhances the compactness and stability of the overall structure of the device. The storage shell 411 and the flip-open top cover 412 facilitate the storage of the adsorption head 251, and the operating frame 421 facilitates the movement and operation of the device by a single operator, thus improving the ease of use of the device.

[0038] Reference Figure 2 , Figure 12 , Figure 13 and Figure 14 The quantitative liquid addition tank assembly 5 includes a horizontal slide 51, a transparent measuring cylinder 52, and a large-diameter dispensing nozzle 53. The horizontal slide 51 is slidably mounted on the snap-fit ​​housing 41. The transparent measuring cylinder 52 is fixedly mounted on the upper end face of the horizontal slide 51, and a capacity scale is provided on the outer side of the transparent measuring cylinder 52. The large-diameter dispensing nozzle 53 is fixedly mounted at the center of the lower end face of the transparent measuring cylinder 52, and a control valve is installed in the large-diameter dispensing nozzle 53. A top cover 521 is fixedly mounted on the upper end face of the transparent measuring cylinder 52. A flip cover 520 is installed on the upper end face of the top cover 521, and a functional shell 522 is integrally formed on the lower end face of the top cover 521. A sampling tube 523 extending into the transparent measuring cylinder 52 is sealed and connected to the lower end face of the functional shell 522. A one-way valve for dispensing liquid is installed at the head of the sampling tube 523, and a manual sampling component 8 is provided in the functional shell 522. The quantitative liquid addition tank assembly 5 comprises a horizontal slide 51, a transparent measuring cylinder 52, and a large-diameter dispensing nozzle 53. The horizontal slide 51 can slide on the snap-fit ​​housing 41, facilitating adjustment of the liquid addition position. The volume scale on the outer side of the transparent measuring cylinder 52 allows operators to accurately control the liquid addition volume. The large-diameter dispensing nozzle 53 and control valve enable quick and accurate addition of liquid nitrogen to the required liquid nitrogen tank. The top cover 521 seals and covers the upper part of the transparent measuring cylinder 52, and a flip-top cover 520 is installed on the upper part of the top cover 521 for better liquid dispensing. The combination of the sampling tube 523, the one-way dispensing valve, and the manual sampling component 8 provides more operational methods and functional options for liquid nitrogen sampling.

[0039] Reference Figure 12The drive assembly 6 includes an external motor 61 and a transverse screw 62. The external motor 61 is fixedly mounted on the outer surface of the housing 41. One end of the transverse screw 62 is fixed to the output shaft of the external motor 61, and the inner end of the transverse slide 51 has a threaded hole that mates with the transverse screw 62. The cooperation between the external motor 61 and the transverse screw 62 in the drive assembly 6 allows the external motor 61 to drive the transverse screw 62 to rotate, and the threaded hole inside the transverse slide 51 to move the transverse slide 51, thereby precisely controlling the extension and retraction of the quantitative liquid dispensing tank assembly 5. This design enables precise control of quantitative liquid dispensing, improving the accuracy and efficiency of liquid dispensing.

[0040] Reference Figure 15 and Figure 16 The manual sampling assembly 8 includes an extraction tube 81, an auxiliary chuck 82, and a piston rod 83. The lower end face of the extraction tube 81 is integrally formed with a connector 811 that connects to the sampling tube 523. The auxiliary chuck 82 is fixedly installed at the head of the extraction tube 81. The piston rod 83 is installed in the extraction tube 81 and is in a sealed sliding connection with the extraction tube 81. The structure of the extraction tube 81, auxiliary chuck 82, and piston rod 83 in this first type of manual sampling assembly 8, through the sealed sliding of the piston rod 83 in the extraction tube 81 and the connection between the connector 811 and the sampling tube 523, enables manual extraction of liquid nitrogen. This structure is simple, easy to operate, and allows the manual sampling assembly 8 to be removed after sampling for better handling of the liquid sample.

[0041] Example 2 Reference Figure 1 , Figure 13 , Figure 14 , Figure 17 and Figure 18A small portable liquid nitrogen transport device for embryo reproduction laboratory includes a transport base 1, which includes a movable seat plate 11 and a buffer platform 12. The buffer platform 12 is fixedly installed on the upper surface of the movable seat plate 11. A pump assembly 2 is fixedly installed on the buffer platform 12. A liquid storage tank group 3 connected to the pump assembly 2 is also fixedly installed on the movable seat plate 11. A multi-functional housing 4 is fastened and fixed on the movable seat plate 11. A quantitative liquid addition tank group 5 is slidably installed on the multi-functional housing 4. The quantitative liquid addition tank assembly 5 includes a horizontal slide 51, a transparent measuring cylinder 52, and a large-diameter dispensing nozzle 53. The horizontal slide 51 is slidably mounted on the snap-fit ​​housing 41. The transparent measuring cylinder 52 is fixedly mounted on the upper end face of the horizontal slide 51, and a capacity scale is provided on the outer side of the transparent measuring cylinder 52. The large-diameter dispensing nozzle 53 is fixedly mounted at the center of the lower end face of the transparent measuring cylinder 52, and a control valve is installed in the large-diameter dispensing nozzle 53. A top cover 521 is fixedly mounted on the upper end face of the transparent measuring cylinder 52. A flip cover 520 is installed on the upper end face of the top cover 521, and a functional shell 522 is integrally formed on the lower end face of the top cover 521. A sampling tube 523 extending into the transparent measuring cylinder 52 is sealed and connected to the lower end face of the functional shell 522. A one-way valve for dispensing liquid is installed at the head of the sampling tube 523, and a manual sampling component 8 is provided in the functional shell 522.

[0042] Reference Figure 17 and Figure 18 The manual sampling assembly 8 includes a sampling scoop 801 and a pull rod 802. The upper surface of the sampling scoop 801 has a sampling groove, and a liquid inlet check valve 803 is installed on the inner side of the bottom surface of the sampling scoop 801. The pull rod 802 is vertically fixed to the upper surface of the sampling scoop 801, and a sealing ring 804, which is sealed to the functional housing 522, is provided on the outer surface of the sampling scoop 801. In this second type of manual sampling assembly 8, the sampling scoop 801, pull rod 802, liquid inlet check valve 803, and sealing ring 804 are designed so that the sampling scoop 801 is pulled by the pull rod 802, and the liquid inlet check valve 803 controls the entry of liquid nitrogen, thus achieving manual sampling. The sealing ring 804 ensures a tight seal during the sampling process, preventing liquid nitrogen leakage. When the operator moves the sampling scoop 801 upward by pulling the lever 802, liquid nitrogen can be adsorbed into the functional shell 522. When liquid nitrogen needs to be taken, the sampling scoop 801 is moved downward, and the liquid nitrogen in the functional shell 522 can enter the sampling groove opened on the upper end face of the sampling scoop 801 through the liquid inlet check valve 803.

[0043] Working principle: The operator pushes the device through the operating frame 421. Because the integrated tray 111 of the movable base plate 11 is equipped with casters 112 near the edge of its lower end face, the device can be flexibly moved to the designated location in the embryology laboratory where liquid nitrogen transfer and filling are required. The insertion screw 125 on the lower end face of the base 121 of the buffer platform 12 is inserted into the positioning hole 113 on the integrated tray 111, ensuring a stable connection between the buffer platform 12 and the movable base plate 11. Simultaneously, the buffer ring 124 cushions vibrations during movement, ensuring the normal operation of all components during movement.

[0044] When replenishing liquid nitrogen, the adsorption head 251 is removed from the housing 411 and placed into the main liquid nitrogen tank. The pump assembly 2 starts working, and the motor inside the pump drives the pump head 23 to rotate in the peristaltic pump housing 21, squeezing the U-shaped hose 22. Liquid nitrogen enters the U-shaped hose 22 through the adsorption head 251 and the adsorption hose 25, and then enters the tank 31 of the liquid storage tank group 3 through the first connecting pipe 221. The auxiliary valve 311 of the liquid storage tank group 3 controls the inlet and outlet of liquid nitrogen and the storage pressure to ensure the safe and stable storage of liquid nitrogen. When the three-way valve 24 opens the second outlet, the liquid is placed into the main tank through the adsorption hose 25 and the adsorption head 251, and the peristaltic pump is started to rotate forward to replenish the liquid storage tank group 3 with liquid nitrogen. In this way, liquid nitrogen can be transported to various liquid nitrogen tanks in the laboratory via the transfer base 1.

[0045] During the quantitative addition of liquid nitrogen, when the first outlet of the three-way valve 24 is opened, the peristaltic pump rotates in the reverse direction to draw liquid nitrogen from the storage tank group 3 into the quantitative addition tank, which then replenishes the liquid nitrogen tank storing the embryos through the quantitative addition tank group 5. Liquid nitrogen enters the three-way valve 24 from the storage tank group 3 via the first connecting pipe 221, the U-shaped hose 22, and the second connecting pipe 222, and then enters the transparent measuring tank 52 of the quantitative addition tank group 5 through the first outlet of the three-way valve 24.

[0046] The operator observes the volume scale on the outer side of the transparent measuring cylinder 52. When the required liquid volume is reached, the pump assembly 2 can be stopped. The horizontal slide 51 slides on the housing 41, adjusting the large-diameter outlet nozzle 53 above the liquid nitrogen tank that needs to be filled. The control valve in the large-diameter outlet nozzle 53 is opened, and liquid nitrogen is quickly and accurately added to the liquid nitrogen tank.

[0047] When sampling liquid nitrogen (optional), if it is necessary to sample liquid nitrogen, the manual sampling component 8 in the functional housing 522 at the lower end of the top cover 521 can be operated. Liquid nitrogen enters the functional housing 522 through the sampling tube 523 and the liquid outlet check valve to realize the liquid nitrogen sampling operation.

[0048] After the filling and sampling operations are completed, close all valves, place the adsorption head 251 into the storage shell 411, and close the top cover 412. The device can then be moved to its storage location for future use.

[0049] The following provides preferred models, materials, and parameter ranges for several key components for reference during selective implementation and further research and development. Note: The parameters below are for preferred reference only and can be adjusted according to actual needs, manufacturer supply, and safety certifications.

[0050] External motor model 61 and driver Recommended models: stepper motors or servo motors, such as the NEMA17 model with high linear accuracy and good torque, for example, the NEMA17 stepper motor with a step angle of 1.8°.

[0051] Rated torque: 0.4–1.5 N·m (depending on the flow resistance of liquid nitrogen and the weight of the metering tank).

[0052] Controller: Paired with microcontrollers / driver boards, such as A4988 or TMC2209 driver chips, it facilitates precise positioning and microstepping control.

[0053] Operating voltage / current: 12–24VDC, current can be finely adjusted via stepper driver.

[0054] Horizontal Screw 62 / Drive Structure Screw type: ball screw or high-precision worm screw, diameter 8–12mm, pitch 2–4mm, ensuring smooth propulsion and high repeatability.

[0055] Screw material: stainless steel (such as 316L) to improve corrosion resistance and low-temperature performance.

[0056] Nut material: low-friction alloy or ceramic coating to reduce wear.

[0057] Base and wheels 112 Casters: Low-temperature resistant, high-quality polyurethane wheels with a diameter of 40–60 mm, equipped with a locking device to ensure stable positioning.

[0058] Base material: aluminum alloy or high-strength engineering plastics (such as PEEK / low-temperature resistant polymers) to reduce weight and improve cold resistance.

[0059] Liquid storage tank group 3 Barrel shell material 31: stainless steel or high-strength composite material to withstand low-temperature impact.

[0060] Auxiliary support block 32 material: polyurethane foam / high-strength plastic, keeping it lightweight while providing cushioning.

[0061] Auxiliary valve 311: Low-temperature resistant safety valve, made of stainless steel or polymer materials, with an operating temperature range of -196°C to room temperature.

[0062] Pump assembly 2 Pump casing material: stainless steel or cold-resistant materials such as high-strength polyimide (PI).

[0063] U-shaped flexible hose 22: Low temperature resistant rubber / silicone, working temperature -196°C to 100°C, bending radius should not be too small to prevent damage.

[0064] Pump head 23: Low-friction, low-temperature resistant injection molded or metal parts to ensure long-term stability.

[0065] Pump motor: small DC motor or servo micro motor, equipped with temperature control protection to prevent overheating.

[0066] Three-way valve 24 Materials: Stainless steel or low-temperature resistant engineering plastics.

[0067] Connection port: The sealing port uses a metal sealing ring 804 or an O-ring 804 to ensure a tight seal at -196°C.

[0068] Control: Electrically controlled switching with short response time ensures no dead zone during operation mode transitions.

[0069] Quantitative dosing tank set 5 The transverse slide 51 is made of aluminum alloy or steel to ensure rigidity during sliding.

[0070] Transparent measuring cylinder 52: made of low-temperature resistant transparent plastic (such as high-density polyethylene HDPE or polypropylene PPO), with volume graduations. The graduation material is chemical-resistant and resistant to low-temperature cracking.

[0071] Large-diameter liquid outlet 53: Stainless steel or high-temperature corrosion-resistant alloy, with polished inner wall to reduce pipe wall residue.

[0072] Top cover 521 and flip cover 520: low temperature resistant plastic / metal combination, with a functional shell 522 inside the top cover 412 to accommodate the sampling tube 523 and the sampling mechanism.

[0073] Sampling tube 523: made of low-temperature resistant stainless steel or low-temperature resistant plastic, with a one-way valve at the head.

[0074] Manual sampling component 8: Used for on-site sampling, with multiple implementation methods (extraction tube 81 head connection, sampling scoop 801, etc.) to meet different sampling volume and speed requirements.

[0075] Preferred implementation form of sampling component Sampling scoop material 801: Low-temperature resistant high-strength polymer or stainless steel, with a sound-absorbing surface to prevent contamination of embryo samples.

[0076] Piston rod 83 seal: Utilizes fluororubber or PTFE sealing ring 804 to ensure sliding sealing performance, maintaining low friction and good sealing even at -196°C.

[0077] Extraction tube 81 head material: stainless steel or high-strength polymer, with tight fit and low temperature resistance.

[0078] Low-temperature safety design: The entire casing is well insulated, and key components are equipped with temperature control, over-temperature protection, and over-voltage protection to ensure safe operation over long periods of time.

[0079] Ease of cleaning and sterilization: The surface of the materials is made of washable materials as much as possible, and the components are detachable for easy daily disinfection and maintenance.

[0080] Data recording and traceability: The volume scale, filling time, sampling records and other data of the quantitative liquid addition tank group 5 can be stored in log form through the controller, which facilitates traceability and compliance review.

[0081] Easy to maintain: The modular design allows for quick disassembly and replacement of components such as the pump assembly 2, the storage tank group 3, and the metering tank group, reducing downtime.

[0082] This invention systematically and modularly designs the core components and working pathways of a small, portable liquid nitrogen transport device for embryo reproductive laboratories. It addresses the shortcomings of existing technologies in terms of portability, quantitative perfusion accuracy, and on-site sampling and recovery, providing a complete and practical solution. This device improves operational efficiency and data traceability while ensuring safety, demonstrating significant practical application value and industrialization potential.

[0083] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A small portable liquid nitrogen transport device for embryo reproduction laboratories, comprising a transport base (1), characterized in that: The transfer base (1) includes a movable base plate (11) and a buffer platform (12). The buffer platform (12) is fixedly installed on the upper surface of the movable base plate (11). A pump assembly (2) is fixedly installed on the buffer platform (12). A liquid storage tank group (3) connected to the pump assembly (2) is also fixedly installed on the movable base plate (11). A multi-functional housing (4) is fastened and fixed on the movable base plate (11). A quantitative liquid addition tank group (5) is slidably installed on the multi-functional housing (4). A drive assembly (6) for driving the quantitative liquid addition tank group (5) to extend and retract is also installed on the multi-functional housing (4).

2. The small-sized portable liquid nitrogen transfer device for embryo laboratory of claim 1, wherein, The movable seat plate (11) includes an integrated tray (111) and casters (112). The casters (112) are installed on the lower end face of the integrated tray (111) near the edge, and the integrated tray (111) has positioning holes (113) for mounting the buffer platform (12).

3. The small portable liquid nitrogen transfer device for embryo laboratory according to claim 2, characterized in that, The buffer platform (12) includes a base (121) and a clamping seat (122). The clamping seat (122) is slidably mounted on the upper end face of the base (121), and a long screw (123) connected to the base (121) is fixedly mounted on the clamping seat (122). A locking nut is installed on the long screw (123). A plug-in screw (125) corresponding to the positioning hole (113) is fixedly mounted on the lower end face of the base (121), and a buffer ring (124) is also fixedly mounted on the lower end face of the base (121).

4. The small-sized portable liquid nitrogen transfer device for embryo laboratory according to claim 3, characterized in that, The pump assembly (2) includes a peristaltic pump housing (21), a U-shaped hose (22), and a pump head (23) that mates with the U-shaped hose (22). The peristaltic pump housing (21) is fixedly installed between the base (121) and the clamping seat (122). The U-shaped hose (22) is fixedly installed in the peristaltic pump housing (21), with both ends of the U-shaped hose (22) extending out of the peristaltic pump housing (21). The pump head (23) is rotatably installed in the peristaltic pump housing (21), and an internal pump motor for driving the pump head (23) to rotate is fixedly installed in the peristaltic pump housing (21). The two ends of the U-shaped hose (22) extend out of the peristaltic pump housing (21). A first connecting pipe (221) and a second connecting pipe (222) are respectively sealed and connected. The other end of the first connecting pipe (221) is sealed and connected to the liquid storage tank group (3). A three-way valve (24) is installed at the other end of the second connecting pipe (222). The inlet of the three-way valve (24) is sealed and connected to the second connecting pipe (222). The first outlet of the three-way valve (24) is sealed and connected to the quantitative liquid addition tank group (5). An adsorption hose (25) is sealed and connected to the second outlet of the three-way valve (24). An adsorption head (251) is sealed and connected to the outer end of the adsorption hose (25).

5. A small portable liquid nitrogen transport device for an embryonic reproductive laboratory according to claim 4, characterized in that, The liquid storage tank group (3) includes a tank shell (31) and an auxiliary support block (32). The auxiliary support block (32) is evenly fixed on the lower end face of the tank shell (31), and the auxiliary support block (32) is fixedly installed on the upper end face of the integrated support plate (111). An auxiliary valve (311) is also installed on the upper end face of the tank shell (31).

6. The small portable liquid nitrogen transfer device for embryo laboratory according to claim 5, characterized in that, The multi-functional housing (4) includes a snap-fit ​​part (41) and a sleeve part (42). The snap-fit ​​part (41) is sleeved and fixed on the integrated tray (111). The sleeve part (42) is fixedly installed on one side of the snap-fit ​​part (41) and sleeved and fixed on the outer side of the barrel shell (31). A storage shell (411) is also fixedly installed on one side of the snap-fit ​​part (41). A flip-open top cover (412) is rotatably installed on the upper end of the storage shell (411). An operating frame (421) is installed on the outer side of the sleeve part (42). The operating frame (421) is rotatably connected to the sleeve part (42).

7. The small portable liquid nitrogen transfer device for embryo laboratory according to claim 6, characterized in that, The quantitative liquid dispensing tank assembly (5) includes a horizontal slide (51), a transparent measuring cylinder (52), and a large-diameter dispensing nozzle (53). The horizontal slide (51) is slidably mounted on the casing (41). The transparent measuring cylinder (52) is fixedly mounted on the upper end face of the horizontal slide (51), and a capacity scale is provided on the outer side of the transparent measuring cylinder (52). The large-diameter dispensing nozzle (53) is fixedly mounted at the center of the lower end face of the transparent measuring cylinder (52), and a control valve is installed in the large-diameter dispensing nozzle (53). The upper end face of the transparent measuring cylinder (52) is fixedly installed with a top cover (521), the upper end face of the top cover (521) is installed with a flip cover (520), and the lower end face of the top cover (521) is integrally formed with a functional shell (522). The lower end face of the functional shell (522) is sealed with a sampling tube (523) that extends into the transparent measuring cylinder (52). The head of the sampling tube (523) is equipped with a liquid discharge check valve, and the functional shell (522) is provided with a manual sampling component (8).

8. The small portable liquid nitrogen transfer device for embryo laboratory according to claim 7, characterized in that, The drive assembly (6) includes an external motor (61) and a transverse screw (62). The external motor (61) is fixedly installed on the outer side of the snap-on housing (41). One end of the transverse screw (62) is fixed to the output shaft of the external motor (61), and the inner end of the transverse slide (51) is provided with a threaded hole that cooperates with the transverse screw (62).

9. The small portable liquid nitrogen transfer device for embryo laboratory according to claim 7, characterized in that, The manual sampling assembly (8) includes an extraction tube (81), an auxiliary chuck (82), and a piston rod (83). The lower end face of the extraction tube (81) is integrally formed with a connector (811) that is connected to the sampling tube (523). The auxiliary chuck (82) is fixedly installed at the head of the extraction tube (81). The piston rod (83) is installed in the extraction tube (81) and is in a sealed sliding connection with the extraction tube (81).

10. A small portable liquid nitrogen transport device for an embryonic reproductive laboratory according to claim 7, characterized in that, The manual sampling assembly (8) includes a sampling scoop (801) and a pull rod (802). The upper surface of the sampling scoop (801) is provided with a sampling groove, and a liquid inlet check valve (803) is installed on the inner side of the bottom surface of the sampling scoop (801). The pull rod (802) is vertically fixed on the upper surface of the sampling scoop (801), and a sealing ring (804) is provided on the outer surface of the sampling scoop (801) to be sealed and connected to the functional housing (522).

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