New applications of avocado pit powder

Abstract

The application belongs to the field of new daily chemical materials, and discloses application of avocado kernel powder in preparation of cosmetics; the cosmetic is a cosmetic with whitening effect; the application finds that the avocado kernel powder has good whitening and lightening effects, and also has antioxidant and soothing effects, and has good use skin feeling and delicate scrubbing feeling, effectively solving the defect that the skin care effect of traditional scrubbing powder is not strong.

Description

Technical Field

[0001] This invention relates to the field of new materials for daily chemical products, and in particular to a new application of avocado kernel powder. Background Technology

[0002] Avocado is mainly used in cosmetics in the form of fruit extract or oil. It is rich in active ingredients such as unsaturated fatty acids, vitamin E, and phytosterols, and has multiple effects such as moisturizing, anti-oxidation, anti-inflammatory and repair. It is suitable for skin care products such as face cream, serum, mask and cleansing products.

[0003] The applicant previously filed an invention patent application, publication number CN117625720A, entitled "An enzymatic hydrolysate of avocado pomace, its preparation method, and its application in the field of cosmetics," which disclosed an enzymatic hydrolysate of avocado pomace. The application effects of this enzymatic hydrolysate of avocado pomace include: anti-oxidation, anti-aging, and soothing and moisturizing effects.

[0004] The applicant subsequently filed another invention patent application, publication number CN121774853A, which is about an avocado peel extract and its preparation method and application. In the application case, the test results of the hyaluronidase inhibition rate of the enzymatic hydrolysis product of avocado kernel were tested, which was approximately 59.11% (D8).

[0005] The applicant has conducted continuous and in-depth research on avocado products, discovered some new experimental phenomena, and thus proposed this invention. Summary of the Invention

[0006] The purpose of this invention is to provide a new application for avocado kernel powder. Our research has found that avocado kernel powder has a good whitening and brightening effect, as well as antioxidant and soothing effects.

[0007] The technical solution of this invention is as follows:

[0008] Application of avocado kernel powder in the preparation of cosmetics; the cosmetics are whitening cosmetics.

[0009] During the comprehensive research on avocados, this invention discovered that avocado kernels have an excellent tyrosinase inhibitory effect. In addition, when avocado kernel powder is added to cosmetics, it can have a scrubbing effect, further enhancing the whitening and brightening effects.

[0010] The significance of this invention lies in the discovery of a solid powder that has a good friction effect on the skin while also having a good whitening and brightening effect. This powder effectively makes up for the deficiency of traditional exfoliating powders added to cosmetics that do not have additional effects, and has good application prospects.

[0011] In the above applications, the cosmetic also has free radical scavenging or skin soothing functions.

[0012] In the above applications, the avocado pit powder has a particle size of 0.15~0.3mm.

[0013] In the above applications, the amount of avocado kernel powder used is 1.5~2.5wt%.

[0014] This application has at least the following beneficial effects:

[0015] This invention discovers a solid powder that has a good friction effect on the skin and also has a good whitening and brightening effect. This powder effectively makes up for the shortcomings of traditional exfoliating powders added to cosmetics that do not have additional effects, and has good application prospects. Attached Figure Description

[0016] Figure 1 The experimental results of chicken embryo testing before and after the sample of the present invention was applied to the cleaned mud film are shown in the figure.

[0017] Figure 2 The experimental results of chicken embryo testing before and after the sample of the present invention was applied to the cleaned mud film are shown in the figure.

[0018] Figure 3 The sample of this invention was applied to an amino acid facial cleanser, and the top 2 radar chart of the questionnaire index results was obtained 14 days later. Detailed Implementation

[0019] The present invention will now be clearly and completely described in conjunction with embodiments thereof. It should be noted that, unless specific conditions are specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all commercially available conventional products.

[0020] I. Preparation method of avocado pit powder

[0021] Step 1: Preparation of dried avocado pit powder: After separating the fresh avocado skin, flesh, and pit (composed of the soft skin and seeds), dry them in an oven at 55℃. Grind the pits for 20 minutes using the grinding function of a Joyoung high-speed blender to obtain coarsely ground avocado pit powder, which is then bottled, labeled, and stored for later use.

[0022] Step 2: Preparation of avocado kernel powder with different particle sizes: First, filter the coarsely ground avocado kernel powder through a 50-mesh (0.3mm) sieve. The upper part is avocado kernel powder with a particle size greater than 50 mesh (0.3mm), which is collected, bagged, and sealed. The lower part of avocado kernel powder with a particle size less than 50 mesh (0.3mm) is then filtered through a 100-mesh (0.15mm) sieve. The upper part is avocado kernel powder with a particle size of 50-100 mesh (0.15mm-0.3mm), which is collected, bagged, and sealed. The lower part is avocado kernel powder with a particle size less than 100 mesh (0.15mm), which is collected, bagged, and sealed.

[0023] Avocado kernel powder particles of >50 mesh, 50-100 mesh, and <100 mesh were used to formulate exfoliating cleansers. Five volunteers were recruited to conduct comparative tests, fill out rating forms, describe the feel of the exfoliating particles, and summarize the parameter scores. Finally, avocado kernel powder particles of 50-100 mesh were selected as the particle size of the formula for functional verification.

[0024] The scoring sheet is shown in Table 1 below;

[0025] Table 1. Volunteer Use Rating Form

[0026]

[0027] This invention also used common solid powders in the cosmetics field, such as bamboo charcoal, red beans, loofah sponge, small-fruited coffee seeds, walnut shells, and strawberry seeds, to prepare comparative samples. The preparation methods for each powder are as follows:

[0028] Bamboo charcoal powder: 100-120 mesh. Bamboo charcoal from the stems of green bamboo in Dabie Mountain, Anhui Province, is dried, pulverized, and then sieved through 100-mesh and 120-mesh screens to obtain the middle layer before sterilization and packaging.

[0029] Red bean powder: 30~50 mesh. After the red beans are dried and pulverized, they are then sieved through 30 mesh and 50 mesh to obtain the middle layer, which is then sterilized and packaged.

[0030] Loofah sponge powder: 16-80 mesh. After drying and pulverizing, the loofah sponge is sieved through 16 mesh and 80 mesh to obtain the middle layer, which is then sterilized and packaged.

[0031] Small coffee seed powder: 40~100 mesh. After the coffee beans are dried and ground, they are then sieved through 40 mesh and 100 mesh to obtain the middle layer, which is then sterilized and packaged.

[0032] Walnut shell powder: 40~100 mesh. After the walnut shells are dried and pulverized, they are then sieved through 40 mesh and 100 mesh to obtain the middle layer, which is then sterilized and packaged.

[0033] Strawberry seed powder: No specific mesh size. Fresh strawberries are crushed, and the seeds and pulp are separated. The powder is then dried, sieved, and finally sterilized and packaged.

[0034] II. Preparation method of avocado kernel extract

[0035] Weigh 10g of dried avocado pit powder into a glass bottle with a ground glass stopper and a cap. Add 200ml of deionized water and a magnetic stir bar. After capping, heat in an 80°C water bath and extract with magnetic stirring for 40 minutes. Cool the extract to room temperature and filter through 100-mesh gauze. Extract the residue again with 200ml of deionized water. Combine the filtrates and centrifuge the extract at 10,000 rpm for 10 minutes. Vacuum filter the supernatant through coarse filter paper, 5µm, 1.2µm, 0.45µm, and 0.22µm filter paper. Place the filtered liquid in a screw-capped, heat-resistant glass bottle and sterilize at 105°C for 10 minutes. Cool to room temperature and add 50% butanediol as a preservative.

[0036] III. Tyrosinase Inhibition Rate Test

[0037] This test involved adding the sample to the facial cleanser base at a concentration of 0.5–2 wt%.

[0038] Facial cleanser base formula reference table 3; test sample reference table 4;

[0039] The testing method is as follows:

[0040] Reagent and Sample Preparation

[0041] Disodium hydrogen phosphate-citric acid buffer solution (pH 6.8): Weigh 14.33 g of disodium hydrogen phosphate and dissolve it in 200 mL of water to prepare a 0.2 mol / L disodium hydrogen phosphate aqueous solution; weigh 2.1 g of citric acid and dissolve it in 100 mL of water to prepare a 0.1 mol / L citric acid aqueous solution; take 154.5 mL of the 0.2 mol / L disodium hydrogen phosphate aqueous solution and add 45.5 mL of the 0.1 mol / L citric acid aqueous solution to prepare a 200 mL disodium hydrogen phosphate-citric acid buffer solution.

[0042] Tyrosinase solution: Weigh 2 mg of tyrosinase and dissolve it in 10 mL of disodium hydrogen phosphate-citrate buffer to prepare a 0.2 mg / mL tyrosinase solution. Prepare immediately before use.

[0043] Levodopa solution: Weigh 10 mg of levodopa and dissolve it in 10 mL of disodium hydrogen phosphate-citrate buffer to prepare a 1 mg / mL levodopa solution. Store in the dark.

[0044] Positive control: kojic acid

[0045] Assay - ELISA reader method

[0046] Take 4 test tubes and number them 1, 2, 3, and 4. Add reagents to each test tube according to Table 2 below:

[0047] Table 2 Test Reagent Information Sheet

[0048]

[0049] Take the ELISA plate and use a 200μL pipette to transfer 200μL of liquid into each well. Place the ELISA plate into the ELISA reader at 475nm for scanning and measurement.

[0050] Data processing

[0051] Calculated according to the formula:

[0052]

[0053] In the formula:

[0054] P – Inhibition rate;

[0055] T—Absorbance of the sample group;

[0056] T0—The background absorbance of the sample;

[0057] C—Absorbance of the enzyme reaction group;

[0058] C0 — absorbance of the solvent background group;

[0059] Experimental validity verification

[0060] A positive control test needs to be added to each batch of experiments. The IC50 of the positive control kojic acid (the sample concentration at which 50% inhibition is achieved) should be between 0.05 mg / mL and 0.15 mg / mL for the test system to be considered effective.

[0061] Table 3 Formula Table

[0062]

[0063] Table 4 Test Sample Information Table

[0064]

[0065] Note: The facial cleanser base material + 2wt% avocado kernel particles (50 mesh) shown in Table 4 refers to the facial cleanser base material as 100% with the addition of 2wt% avocado kernel particles with a particle size of 50 mesh.

[0066] D represents the dilution factor, and D0 represents that the test sample is the original solution.

[0067] Results analysis:

[0068] 1. As can be seen from the test results in Table 4, the formula exhibits excellent tyrosinase inhibition rate after the addition of avocado kernel particles;

[0069] 2.2% avocado seed particles can significantly improve the tyrosinase inhibition rate of the substrate, and the effect is better than other types of particles;

[0070] 3. Avocado kernel extract also showed an effect on tyrosinase inhibition, but the effect was not as good as that of avocado kernel particles. In addition, no synergistic effect was observed when avocado kernel particles and avocado kernel extract were used in combination.

[0071] Based on the above information, we believe that the core value of this invention is the development of a solid particle with particularly good whitening effect.

[0072] IV. In vitro antioxidant test

[0073] The test samples were facial cleansers containing the above-mentioned particles. The facial cleanser formula is shown in Table 3; the amount added is shown in Table 5; and the test results are shown in Table 6.

[0074] The DPPH and ABTS free radical scavenging capabilities of facial cleansers with added particles were tested in accordance with the national standard GB / T 39100-2020, "Determination of Antioxidant Properties of Peptides - DPPH and ABTS Methods".

[0075] The DPPH free radical scavenging rate test method is as follows: Weigh 5.0 mg of DPPH powder, protect from light, sonicate to dissolve in anhydrous ethanol, and bring the volume to 100.0 mL to prepare a 50.0 μg / mL DPPH solution. This solution should be prepared fresh each time. Pipette an appropriate amount of pre-diluted supernatant from the added-particle facial cleanser into a 10 mL volumetric flask, add distilled water to bring the volume to 10 mL, and shake thoroughly. In the experimental group, pipette 200 µL of the test solution and 600 µL of DPPH solution (50.0 μg / mL) into a 2 mL disposable centrifuge tube. In the blank group, pipette 200 µL of distilled water and 600 µL of anhydrous ethanol into a 2 mL disposable centrifuge tube. Mix thoroughly using a vortex mixer, react at room temperature in the dark for 30 min, then centrifuge for 5 min. Pipette 180 µL of the supernatant from centrifugation into a 96-well plate, and measure the absorbance at 517 nm using a microplate reader.

[0076] The ABTS free radical scavenging rate test method is as follows: Accurately weigh 200.0 mg of 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (hereinafter referred to as ABTS) and 34.4 mg of potassium persulfate using an analytical balance, dissolve them in 50.0 mL of distilled water, shake well, and let stand at room temperature in the dark for 24 h to obtain the ABTS stock solution. Take an appropriate amount of the ABTS stock solution and dilute it with 95% ethanol until the absorbance value is within 0.7 ± 0.02 to obtain the ABTS assay solution. This solution should be prepared and used immediately. Use a pipette to take an appropriate amount of the pre-diluted supernatant of the added particle facial cleanser into a 10 mL volumetric flask, add distilled water to make up to 10 mL, and shake well before use. In the experimental group, 100 µL of the test solution and 900 µL of ABTS solution were pipetted into a 2 mL disposable centrifuge tube. In the blank group, 100 µL of distilled water and 900 µL of 95% ethanol were pipetted into a 2 mL disposable centrifuge tube. After mixing thoroughly with a vortex mixer, the mixture was incubated at room temperature in the dark for 5 min, centrifuged for 5 min, and 180 µL of the supernatant was pipetted into a 96-well plate. The absorbance was measured using a microplate reader at a wavelength of 734 nm.

[0077] Table 5 Test Sample Information Table

[0078]

[0079] Note: D in Table 5 represents the dilution factor of the facial cleanser. For example, in the test sample shown in NO.2, the test sample for DPPH free radical scavenging rate is a sample of "facial cleanser base + 2% avocado kernel particles (50 mesh)" diluted 8 times.

[0080] Results analysis: The facial cleanser base with 2% avocado pit particles showed the highest DPPH and ABTS free radical scavenging rates, which were consistent with its tyrosinase inhibition ability.

[0081] V. In vitro soothing test

[0082] Test Method: Weigh an appropriate amount of sample, dissolve and dilute with deionized water to different concentrations to obtain test sample solutions of different concentrations. Transfer different volumes of the test solution, standard enzyme solution, negative and positive solutions into 96-well plates as the experimental group, blank group, negative group, and positive group, respectively. Transfer each solution to a 2 mL disposable centrifuge tube and vortex to mix. Incubate at 37℃ for 30 min, then in a boiling water bath for 5 min. Take a certain amount of the reaction solution and add DNS solution to a new 2 mL disposable centrifuge tube. Incubate in a boiling water bath for 5 min. Add 200 μL to a 96-well plate. Scan the plate at 540 nm using a microplate reader and calculate the OD value.

[0083]

[0084] In the formula:

[0085] P – Inhibition rate

[0086] Asam-s — Absorbance of the sample test group;

[0087] Asam-c — Absorbance of the blank sample group;

[0088] Ab—Absorbance of the enzyme solution group;

[0089] A0—Absorbance of the reagent blank;

[0090] The test results are shown in Table 6;

[0091] Table 6 Results of Hyaluronidase Inhibition Rate Test

[0092] Experimental group Physicochemical soothing (hyaluronidase inhibition experiment) Cleansing mud mask D0:<0 Cleansing mud mask + 2% avocado pit particles D0:38.16% Cleansing mud mask + 2% avocado pit extract D0:34.02% Cleansing mud mask + 2% red bean particles D0:<0

[0093] The tests above demonstrate that avocado pit particles also show significant advantages in terms of soothing effects.

[0094] VI. Biosafety Testing

[0095] A cleansing mud mask with 2% 50-mesh avocado pit particles was tested on chicken embryos.

[0096] Eye irritation test (chicken embryo test) method: Using the enzymatic hydrolysis supernatant of avocado peel as the sample, the chicken embryo safety and irritation of the sample are tested through the chicken embryo test. The test method is based on SN / T 2329-2009 Chicken embryo chorioallantoic membrane test for eye irritation / corrosiveness in cosmetics, and the specific method is as follows:

[0097] 1) CAM Preparation: Candling of 9-day-old chicken embryos was performed. The air cell portion of the shell was removed using dental serrated forceps to expose the white membrane, handling it carefully to avoid damaging its integrity. A drop of 0.9% sodium chloride (NaCl) solution was added with a pipette to moisten the membrane, and the inner membrane was carefully removed with forceps, ensuring the vascular membrane remained undamaged. The structure of the vascular system was then observed again, and its integrity and suitability for the experiment were assessed.

[0098] 2) Pre-experiment test: Take 2 chicken embryos for the experiment to check the reactivity of this batch of chicken embryos and determine whether the reaction time method should be used in the formal experiment. The reaction time is limited to 5 minutes.

[0099] 3) Preliminary test: Using 6 chicken embryos, take 0.3 mL of the test substance, ensuring that at least 50% of the CAM surface is covered by the test substance. Immediately after the test substance is applied, observe the CAM reaction for 5 minutes and record the results. Determine whether the test substance is suitable for this method and determine whether the reaction time method or the endpoint evaluation method should be used in the formal test.

[0100] The formula for cleansing mud masks is shown in Table 7.

[0101] Table 7 Formulation Table

[0102]

[0103] Test results are for reference. Figure 1 and Figure 2 The test results showed that neither the cleansing mud mask base nor the cleansing mud mask containing avocado pit particles caused any irritation.

[0104] VII. Volunteer Test

[0105] 7.1 Consumer testing process for avocado seed cleanser:

[0106] I. Test Plan:

[0107] 1.1 Test Sample

[0108] Avocado seed amino acid facial cleanser, with an avocado seed content of 2 wt%;

[0109] 1.2 Testing Process

[0110] Consumer testing involves distributing test products to qualified consumers and having them use the products at home according to the product's usage instructions and their actual usage habits. After a certain period of continuous use, the effectiveness of the test products is evaluated based on the consumers' subjective feelings about the products through methods such as interviews, questionnaires, and consumer diaries.

[0111] 1.3 Test Evaluation Format

[0112] This test used a questionnaire to observe and record the consumer evaluation process. For details of the questionnaire, please see Appendix II.

[0113] 1.4 Parameter Meaning

[0114] The questionnaire options are divided into five levels: very dissatisfied / very unpleasant, somewhat dissatisfied / somewhat unpleasant, neutral, somewhat satisfied / somewhat mild, and very satisfied / very mild.

[0115] The top two categories are the percentage of people who rated themselves as "relatively satisfied / relatively mild" plus those who rated themselves as "very satisfied / very mild".

[0116] II. Data Analysis

[0117] Data is presented as a percentage.

[0118] 2.1 Descriptive Statistics

[0119] Calculate the percentage of each option for the tested product in each questionnaire indicator, especially the percentage of the top two.

[0120] 2.2 Evaluation Criteria

[0121] At any survey point after the product was used, for each evaluation indicator on the questionnaire, if the percentage of respondents who agreed that the product was satisfactory / mild was higher than the percentage of respondents who disagreed / did not agree, and a binomial distribution test (proportion 0.5) showed a statistically significant difference between the number of respondents who expressed satisfaction / mildness and those who disagreed / did not agree (p<0.050), then the respondents agreed that the product had the corresponding effect in this consumer use test. If the statistical significance level of the number of respondents who expressed satisfaction / mildness and those who disagreed / did not agree (p≥0.050), then the respondents did not agree that the product had the corresponding effect in this consumer use test.

[0122] Note: Satisfied number = Number of people who selected "Very Satisfied" + Number of people who selected "Somewhat Satisfied"; Dissatisfied number = Number of people who selected "Neutral" + Number of people who selected "Somewhat Dissatisfied" + Number of people who selected "Very Dissatisfied"; Moderate number = Number of people who selected "Very Moderate" + Number of people who selected "Neutral"; Unmoderate number = Number of people who selected "Neutral" + Number of people who selected "Unmoderate" + Number of people who selected "Very Unmoderate"; III. Test Results:

[0123] Refer to Table 8, which shows the results of various questionnaire indicators after the subjects used avocado seed particle facial cleanser continuously for 14 days.

[0124] Table 8 Statistical Results of Questionnaire Indicators

[0125] Evaluation indicators after 14 days Very dissatisfied / Very unpleasant Dissatisfied / Not very gentle generally Quite satisfied / Quite mild Very satisfied / Very mild TOP2 P (Satisfied / Moderate vs. Unsatisfied / Non-Moderate) Particle size satisfaction 0.00% 0.00% 23.81% 52.38% 23.81% 76.19% ＜0.050 Particle friction satisfaction 0.00% 0.00% 19.05% 61.90% 19.05% 80.95% ＜0.050 Product mildness and satisfaction 0.00% 0.00% 0.00% 52.38% 47.62% 100.00% ＜0.050 Satisfaction with particle cleaning effect 0.00% 0.00% 19.05% 47.62% 33.33% 80.95% ＜0.050 Overall satisfaction with particles 0.00% 0.00% 19.05% 42.86% 38.10% 80.95% ＜0.050 Overall product satisfaction 0.00% 0.00% 23.81% 52.38% 23.81% 76.19% ＜0.050

[0126] Note: Each option for the evaluation indicator is "Very Dissatisfied / Very Unsatisfactory", "Somewhat Dissatisfied / Somewhat Unsatisfactory", "Average", "Somewhat Satisfied / Somewhat Satisfactory", or "Very Satisfied / Very Satisfactory".

[0127] TOP2 = "Very satisfied / Very mild" + "Somewhat satisfied / Somewhat mild";

[0128] Figure 3 A radar chart showing the top two indicators from the 14-day questionnaire.

[0129] IV. Test Conclusion

[0130] In this consumer testing, 21 participants used the avocado seed amino acid facial cleanser twice daily, morning and evening, for 14 consecutive days. The results of their self-evaluation questionnaires are as follows:

[0131] 4.1 After 7 days of use

[0132] 1) 76.19% of the subjects were satisfied with the particle size after using avocado kernel particle amino acid facial cleanser for 14 days, and there was a statistically significant difference between the number of people who expressed satisfaction and those who were not satisfied (p<0.050).

[0133] 2) 80.95% of the subjects were satisfied with the avocado kernel particle amino acid facial cleanser after 14 days of use, and there was a statistically significant difference between the number of people who expressed satisfaction and those who were not satisfied (p<0.050).

[0134] 3) 100% of the subjects were satisfied with the gentleness of the product after using the avocado kernel particle amino acid cleanser for 14 days, and there was a statistically significant difference between the number of people who expressed satisfaction and those who were not satisfied (p<0.050).

[0135] 4) 80.95% of the subjects were satisfied with the cleansing effect of the avocado kernel particle amino acid facial cleanser after 14 days, and there was a statistically significant difference between the number of people who expressed satisfaction and those who were not satisfied (p<0.050).

[0136] 5) 80.95% of the subjects were satisfied with the overall satisfaction of the particles after using avocado kernel particle amino acid cleanser for 14 days, and there was a statistically significant difference between the number of people who expressed satisfaction and those who were not satisfied (p<0.050).

[0137] 6) 76.19% of the subjects were satisfied with the overall product satisfaction after using the avocado kernel particle amino acid facial cleanser for 14 days, and there was a statistically significant difference between the number of people who expressed satisfaction and those who were not satisfied (p<0.050).

[0138] 7.2 Human testing process for avocado pit particle mud film:

[0139] On the day of the visit, after washing the inside of the subjects' arms with facial cleanser, two 4cm*4cm areas were selected on the inside of each subject's left and right arms and marked as No. 1-4. Skin-related indicators were measured before histamine stimulation. Then, the test sites were stimulated with a membrane soaked in 1.5% histamine solution for 15 minutes, and after removing the membrane, the subjects waited for 10-15 minutes and measured skin-related indicators after stimulation. Finally, samples were applied to the marked sites at a dosage of 0.02g / cm2. Avocado seed particle mud film was applied to No. 1 on the left and No. 2 on the right, while No. 2 on the left served as a blank group and no mud film was applied. No. 1 on the right was applied with mud film base material. After 15 minutes, the samples were washed off with water and skin-related indicators were measured.

[0140] The test results are shown in Table 9.

[0141] Table 9 Human Test Results

[0142]

[0143] The test results above show that when the product of this invention is applied to facial cleanser, it can better inhibit histamine stimulation and has a significant soothing effect compared to the base material.

Claims

1. Use of avocado pit powder in the preparation of a cosmetic product; the cosmetic product is a cosmetic product with whitening effect.

2. Use according to claim 1, characterized in that, The cosmetic product also has a free radical scavenging function or a skin soothing function.

3. Use according to claim 1, characterized in that, The particle size of the avocado pit powder is 0.15-0.3 mm.

4. Use according to claim 1, characterized in that, The amount of avocado pit powder in the cosmetic product is 1.5-2.5 wt%.

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