A composition synergistically realizing optical lightening and long-acting moisturizing, and preparation and application thereof

Abstract

This invention discloses a composition that synergistically achieves optical brightening and long-lasting moisturizing, as well as its preparation and application, belonging to the field of biomedical industry technology. The composition comprises an immediate brightening combination and a long-lasting hydrating combination. The immediate brightening combination consists of anti-yellowing, anti-redness, and brightening ingredients, along with a nano-vesicle carrier. The long-lasting hydrating combination consists of epidermal plumping ingredients, dermal reconstruction ingredients, and cell activating ingredients. This invention achieves immediate brightening through a triangular synergistic effect of anti-yellowing, anti-redness, and brightening via "carnosine + sodium heparin + niacinamide + milk phospholipid vesicles." It constructs a three-tiered hydrating system of epidermis-dermis-cell through "sodium hyaluronate + globular collagen + microsphered PDRN," achieving a deep coupling of 1-hour immediate brightening and 48-hour long-lasting moisturizing. Furthermore, the vesicle encapsulation process reduces the irritation of niacinamide, making it suitable for sensitive skin, with a lightweight feel, and exhibiting significant synergistic effects.

Description

Technical Field

[0001] This invention belongs to the field of biomedical industry technology, specifically relating to a composition that synergistically achieves optical brightening and long-lasting moisturizing, as well as its preparation and application. Background Technology

[0002] In recent years, with the popularization of the "natural beauty" concept, consumers have placed higher demands on the efficacy of skin repair agents, urgently requiring products that can simultaneously achieve "instant skin brightening" and "long-lasting hydration." However, existing technologies still have significant shortcomings in achieving deep integration and synergistic effects of these two functions. Currently, the main technical paths for achieving instant optical brightening in the field of skin repair agents include: 1) Physical covering type. Represented by inorganic pigments such as titanium dioxide (TiO2) and zinc oxide (ZnO), instant whitening effects are achieved through optical scattering. For example, Chinese patent application CN111349688A discloses a "no-makeup cream" that uses optical colorants such as titanium dioxide and mica to achieve instant brightening. Although this path can quickly brighten the skin, it suffers from problems such as heavy coverage, unnatural makeup look, and easy clogging of pores, making it difficult to meet the need for naturally clear skin. 2) Exfoliating type. Brightening is achieved by exfoliating the stratum corneum using salicylic acid, fruit acid, azelaic acid, and proteases. Long-term use can damage the skin barrier, leading to decreased skin tolerance, and is especially unsuitable for the growing population with sensitive skin. 3) High-activity whitening type. This approach uses high doses of ingredients such as niacinamide, tranexamic acid, and arbutin for potent intervention. While this method has some effect, its safety is questionable, easily causing irritation (such as niacinamide intolerance), making it difficult to popularize as a daily skincare product. In summary, existing technologies have the following core shortcomings in achieving the synergistic effect of "instant brightening + long-lasting hydration": The synergistic effects are disconnected. Brightening and moisturizing ingredients are mostly simple physical mixtures, lacking a synergistic mechanism that can bridge the gap between immediate optical brightening and long-lasting hydration. Their effects are independent of each other and cannot form a complementary and enhancing closed loop of "hydration promotes brightening, and brightening reveals hydration".

[0003] There are misconceptions regarding the core efficacy. Regarding "brightening," traditional techniques simply equate it with "whitening," neglecting the three-dimensional balance of "transparency, evenness, and rosiness" in Eastern skin aesthetics. They fail to specifically address the "dullness" caused by advanced glycation end products (AGEs), which account for over 40% of skin dullness, and the "dirty redness" caused by capillary dilation. Regarding "hydration," traditional techniques simplify it to simply increasing skin moisture content, ignoring the structural and functional foundations such as extracellular matrix fullness and cellular self-hydration capacity. They lack a progressively layered, three-tiered hydration system design, from the epidermis to the dermis and then to the cells.

[0004] Balancing skin feel and efficacy is difficult. Increasing optical powder to achieve immediate brightening can lead to a heavy texture and dry skin feel; increasing oil content to enhance moisturization can interfere with powder dispersibility, creating a technical dilemma where "brightening-moisturizing-skin feel" cannot be achieved simultaneously.

[0005] Insufficient safety and gentleness. Long-term use of high-concentration whitening ingredients or acid-based exfoliants can easily damage the skin barrier. In particular, ingredients such as niacinamide may cause intolerance to niacin, failing to meet the safety needs of sensitive skin groups for "makeup and skincare in one" and "gentle and effective".

[0006] Therefore, how to develop a composition that can simultaneously achieve immediate skin brightening and long-lasting hydration, while also having a good skin feel and high safety, and establish a deep synergistic mechanism between the two, is a technical problem that urgently needs to be solved in this field. Summary of the Invention

[0007] In view of the technical problems of existing skin repair preparations, such as the disconnect between immediate brightening and long-lasting moisturizing effects, the difficulty in balancing skin feel and efficacy, and the unfriendly effect of high concentrations of whitening ingredients on sensitive skin, this invention aims to provide a composition that synergistically achieves optical brightening and long-lasting moisturizing, as well as its preparation and application.

[0008] To achieve the above objectives, the present invention employs the following technical solution: In a first aspect, the present invention provides a composition for synergistically achieving optical brightening and long-lasting moisturizing, comprising, by weight parts: xanthan gum: 0.01-0.5 parts; polyacrylamide® milk phospholipid vesicle extract: 0.1-5.0 parts; niacinamide: 0.5-5.0 parts; sodium heparin: 0.01-0.2 parts; carnosine: 0.05-0.5 parts; sodium hyaluronate: 0.01-0.3 parts; polyacrylamide® spherical collagen: 0.05-8.0 parts; polyacrylamide® microsphereized PDRN: 0.05-10.0 parts; moisturizer: 1-30 parts; skin feel modifier: 0.01-1.5 parts; purified water to make up to 100 parts.

[0009] The polyacrylamide® milk phospholipid vesicle extract, niacinamide, sodium heparin, and carnosine together constitute an instant brightening combination; the sodium hyaluronate, polyacrylamide® spherical collagen, and polyacrylamide® microsphered PDRN together constitute a long-lasting hydrating combination.

[0010] The moisturizer is selected from one or more of glycerin, 1,2-pentanediol, and ethylhexylglycerin. This polyol moisturizer not only provides immediate hydration but also works synergistically with xanthan gum to form a translucent texture, avoiding the greasy feel or uneven powder dispersion problems associated with traditional high-oil formulations.

[0011] The skin feel modifier is methyl gluceth-20. This ingredient can significantly reduce the stickiness of the composition, and when combined with xanthan gum, it forms a light and moisturizing application experience, solving the technical defects of existing instant brightening products that are "heavy, dry, and prone to clogging pores".

[0012] The microsphere-sized PDRN of the aforementioned Polyglycin® has a particle size of 110-130 nm; the microsphere-sized collagen of the aforementioned Polyglycin® has a particle size of less than 120 nm. This nanoscale particle size allows macromolecular active ingredients to penetrate the stratum corneum and reach the dermis. Experiments have shown that the composition containing PDRN and microsphere-sized collagen within this particle size range significantly improves elasticity after 28 days compared to the composition without microsphere technology, thus solving the industry pain point of difficult transdermal penetration and easy degradation of medical aesthetic ingredients.

[0013] The Polymicro® milk phospholipid vesicle extract is a nanoscale vesicle structure with a phospholipid bilayer membrane. This structure can effectively encapsulate niacinamide during high-temperature and high-pressure homogenization. Safety tests have shown that the vesicle structure significantly reduces the irritation of niacinamide, making it safe for people with sensitive skin to use.

[0014] Secondly, the present invention provides a method for preparing the composition that synergistically achieves optical brightening and long-lasting moisturizing, comprising the following steps: Xanthan gum, humectant and purified water were mixed, heated and stirred until homogeneous to obtain phase A; Polytetrafluoroethylene® milk phospholipid vesicle extract, nicotinamide, heparin sodium, carnosine and purified water were mixed and homogenized under high pressure to obtain phase B; Sodium hyaluronate, polyglucono-polystyrene® spherical collagen, polyglucono-polystyrene® microsphereized PDRN and purified water were mixed to obtain phase C; Cool phase A, then add phases B and C sequentially, and stir at high speed to obtain a composition that provides immediate optical brightening and long-lasting water-light synergy.

[0015] The heating and stirring temperature is 70 ℃~85 ℃, the high-pressure homogenization temperature is 75 ℃~85 ℃, the pressure is -0.09~-0.1 MPa, and the homogenization is performed 5 to 10 times, each time for 1 min to 2 min.

[0016] Phase A is cooled to 30℃~45℃, and the high-speed stirring speed is 2000 rpm~5000 rpm for 15 min~30 min. This ensures that PDRN and collagen do not undergo thermal denaturation during preparation, while high-speed stirring ensures that the brightening and hydrating components are uniformly dispersed without damaging the vesicle structure.

[0017] Thirdly, the present invention provides a skin repair preparation comprising the aforementioned composition that synergistically achieves optical brightening and long-lasting moisturizing.

[0018] Compared with the prior art, the present invention achieves the following technical effects: The composition provided by this invention, which synergistically achieves optical brightening and long-lasting moisturizing, integrates for the first time the triangular synergistic mechanism of "removing yellowing-removing redness-brightening" with the three-level water-light plumping system of "epidermis-dermis-cells" into the same formula. Experimental data shows that the composition significantly increases the L value and significantly decreases the a and b values ​​within 1 hour, and maintains the skin moisture content above 69.1% and the TEWL value as low as 7.0 after 48 hours. At the same time, the elasticity R2 value increases by more than 60%, achieving a deep coupling of immediate brightening and long-lasting moisturizing.

[0019] The preparation method provided by this invention involves a stepwise process of phase A-phase-phase-phase-phase-phase-phase. First, a thickening matrix is ​​constructed. Then, a brightening complex encapsulated in vesicles is formed through high-pressure homogenization. Finally, active water-light components are added at low temperature. This method effectively protects the activity of heat-sensitive components PDRN and collagen, while reducing the irritation of niacinamide. The process is simple, highly reproducible, and suitable for industrial production.

[0020] The skin repair formulation containing the composition provided by this invention, when applied to skin repair formulations such as ointments, gels, and films, can simultaneously provide the product with the dual effects of "1-hour instant optical brightening" and "48-hour long-lasting hydration," and is friendly to sensitive skin with a light and thin texture. It meets consumers' core demand for skin repair formulations that combine nourishment and care, and has broad market application prospects. Detailed Implementation

[0021] To enable those skilled in the art to better understand the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present invention.

[0022] For experiments not specifically described in the examples, the procedures or conditions should be followed according to the conventional experimental procedures described in the literature in this field. Reagents or instruments whose manufacturers are not specified are all commercially available conventional reagent products.

[0023] Polymicro® Milk Phospholipid Vesicle Extract: refers to nanoscale vesicles with a phospholipid bilayer structure extracted from fresh milk, with a particle size ranging from 50 to 200 nm. These vesicles can encapsulate and deliver active ingredients, improving their stability and transdermal absorption rate. The Polymicro® Milk Phospholipid Vesicle Extract used in this invention was purchased from Microsec Biotechnology (Yantai) Co., Ltd.

[0024] Polyglycinate® microspheres of PDRN: These are polydeoxyribonucleic acid (PDRN) microspheres prepared using a stacked microsphere controlled-release technology. The PDRN molecular weight ranges from 50 to 1500 kDa, and the microspheres have a particle size of 121 nm. This technology assembles PDRN into structurally stable microspheres through weak intermolecular interactions, thereby improving transdermal absorption and activity retention. The polyglycinate® microspheres of PDRN used in this invention were prepared according to patent CN117017824B and purchased from Baihong Synthetic Biotechnology (Yantai) Co., Ltd. Polysaccharide® Spherical Collagen: refers to a nanoscale spherical structure formed by compressing humanized type III recombinant collagen through a layered microsphere controlled-release technology, with a particle size of less than 120 nm. This structure enables transdermal absorption of collagen, which dissociates after entering the dermis and plays a role in rebuilding the collagen fiber network. The Polysaccharide® Spherical Collagen used in this invention was purchased from Baihong Synthetic Biotechnology (Yantai) Co., Ltd. Sodium heparin: purchased from Hebei Changshan Kailuonite Biotechnology Co., Ltd., CAS: 9041-08-1; Carnosine: Symrise, CAS: 305-84-0; Nicotinamide: DSM, 100% purity; Sodium hyaluronate: purchased from Shandong Anhua, molecular weight 200,000-500,000; Xanthan gum: purchased from CPKelco; the remaining excipients are all conventional raw materials in the field of skin repair preparations.

[0025] Unless otherwise specified, all experimental materials used in this invention are commercially available products well known to those skilled in the art.

[0026] Example 1: A composition that synergistically achieves optical brightening and long-lasting moisturizing This embodiment provides a composition that synergistically achieves optical brightening and long-lasting moisturizing, comprising the following raw materials by weight: 0.2 parts xanthan gum, 2 parts glycerin, 2.8 parts 1,2-pentanediol, 1.5 parts ethylhexylglycerin, 0.2 parts methyl gluceth-20, a long-lasting composition (1.5 parts polyglycinate® microsphere PDRN + 1.0 part polyglycinate® spherical collagen + 0.1 part sodium hyaluronate), a short-lasting composition (2.0 parts polyglycinate® milk phospholipid vesicle extract + 0.1 part carnosine + 0.1 part sodium heparin + 0.5 parts niacinamide), and purified water to a final volume of 100 parts. The specific preparation method is as follows: Xanthan gum, glycerol, 1,2-pentanediol, ethylhexylglycerol, methyl gluceth-20 and 60 parts of purified water were mixed and heated to 75-80°C and stirred until homogeneous to obtain phase A. The mixture was then cooled to 40°C for later use.

[0027] Polytetrafluoroethylene® milk phospholipid vesicle extract, nicotinamide, heparin sodium, carnosine and 10 parts purified water were mixed and homogenized 8 times for 2 minutes each time by a high pressure homogenizer at -0.08 MPa pressure and 80℃ to obtain phase B.

[0028] Sodium hyaluronate, polyglucono-polysaccharide® spherical collagen, polyglucono-polysaccharide® microsphereized PDRN, and 10 parts purified water were mixed evenly to obtain phase C.

[0029] Under stirring conditions, phases B and C were added to phase A in sequence and stirred at high speed (3000 rpm) for 20 min. Then, purified water was added to make up to 100 parts and stirred evenly to obtain a composition with immediate optical brightening and long-lasting water-light synergy.

[0030] Example 2 Based on Example 1, this embodiment provides a composition that synergistically achieves optical brightening and long-lasting moisturizing, comprising the following raw materials by weight: 0.1 parts xanthan gum, 2 parts glycerin, 2.8 parts 1,2-pentanediol, 1.5 parts ethylhexylglycerin, 0.2 parts methyl gluceth-20, 1.0 part polytetrafluoroethylene® milk phospholipid vesicle extract, 1.0 part niacinamide, 0.05 parts sodium heparin, 0.2 parts carnosine, 0.5 parts polyglycinate® microsphere PDRN, 0.5 parts polyglycinate® globular collagen, 0.05 parts sodium hyaluronate, and purified water to make up to 100 parts.

[0031] The specific preparation method is the same as in Example 1.

[0032] Example 3 Based on Example 1, this embodiment provides a composition that synergistically achieves optical brightening and long-lasting moisturizing, comprising the following raw materials by weight: 0.4 parts xanthan gum, 2 parts glycerin, 2.8 parts 1,2-pentanediol, 1.5 parts ethylhexylglycerin, 0.2 parts methyl gluceth-20, 5.0 parts polytetrafluoroethylene® milk phospholipid vesicle extract, 2.5 parts niacinamide, 0.15 parts sodium heparin, 0.3 parts carnosine, 5.0 parts polyglucono-Pacific® microsphere PDRN, 3.0 parts polyglucono-Pacific® spherical collagen, 0.2 parts sodium hyaluronate, and purified water to make up to 100 parts.

[0033] The specific preparation method is the same as in Example 1.

[0034] Example 4 This embodiment, based on Example 1, provides a composition that synergistically achieves optical brightening and long-lasting moisturizing. The raw material composition is the same as in Example 1, the only difference being that the high-pressure homogenization conditions in the preparation method are: 80 MPa, 85°C, and 5 cycles of homogenization. The remaining steps are the same as in Example 1.

[0035] Comparative Examples 1-9 The comparative example was prepared according to the formula in Table 1. The preparation method was basically the same as in Example 1, except that some components were missing or high-pressure homogenization was not performed (see the table below for the specific formula, where the numbers are parts by weight, and purified water was added to 100).

[0036] Table 1: Formulas for each comparative example

[0037] Note: Comparative Example 1 is a blank matrix; Comparative Example 2 lacks globular collagen and sodium hyaluronate; Comparative Example 3 lacks PDRN; Comparative Example 4 is a complete long-acting combination; Comparative Example 5 is a brightening combination without vesicle encapsulation (without milk phospholipid vesicles); Comparative Example 6 only contains milk phospholipid vesicles; Comparative Example 7 is a complete short-acting combination (without long-acting combination); Comparative Example 8 only contains short-acting combination; Comparative Example 9 only contains long-acting combination.

[0038] II. Test Methods and Results 1. Test Subjects and Conditions We recruited female volunteers aged 20-55, 10 in each group, for a total of 100 participants. Testing environment: temperature 22℃±1℃, humidity 50%±5%. Participants cleaned their faces and sat quietly for 30 minutes, then received 2.0 mg / cm³. 2 The sample was applied evenly. Subjects included individuals with dull skin tone, weakened skin barrier function, or insufficient skin radiance. Tests were performed before use and at 1, 2, 4, 6, 8, 24, and 48 hours after use. All data were calculated as the average of three parallel measurements.

[0039] 2. Instant brightening effect (Lab value measurement) Using a Demelab combo single-microphone tester, L value (brightness, the higher the value, the brighter the skin), a value (redness, the lower the value, the less redness), and b value (yellowness, the lower the value, the less yellowing) were measured before application (initial value) and at 1h, 2h, 8h, and 24h after application. The results are shown in Table 2.

[0040] Table 2: Changes in Lab values ​​of different samples over time

[0041] As shown in Table 2, the Lab values ​​of Comparative Example 1 did not differ significantly from the initial values ​​at 1h, 2h, 8h, and 24h. Comparative Example 5 (without Polytetrafluoroethylene® milk phospholipid vesicle extract, containing only carnosine, heparin sodium, and niacinamide) had higher L and b value change rates than Comparative Example 6, but lower a value change rate. This indicates that without Polytetrafluoroethylene® milk phospholipid vesicle extract, niacinamide would damage the skin barrier, resulting in minimal change in the redness value of Comparative Example 5. This also suggests that the components of the short-acting brightening composition have a synergistic effect. Comparative Example 6 (without carnosine, heparin sodium, and niacinamide, containing only Polytetrafluoroethylene® milk phospholipid vesicle extract) had a slightly higher Lab value change rate than Comparative Example 1, indicating that Polytetrafluoroethylene® milk phospholipid vesicle extract has a certain brightening effect. In Example 1, the rate of change of Lab value showed the best efficacy. Therefore, the short-term brightening composition of Polymicro® milk phospholipid vesicle extract, carnosine, niacinamide, and heparin sodium needs to work synergistically to achieve both short-term brightening effect and to improve the tolerance of niacinamide.

[0042] 3. Long-lasting hydration effect (skin elasticity and radiance) Using a cutometer, skin elasticity parameters R2 (net elasticity, rebound ability without negative pressure), R5 (elasticity ratio, reflecting skin viscoelasticity), and R7 (rebound ability, immediate rebound rate after the removal of negative pressure) were measured before use (D0), 14 days after use (D14), and 28 days after use (D28). Each area was measured three times in parallel, and the average value was taken. The rate of change (%) compared to D0 was calculated. Simultaneously, subjects rated their overall glossiness satisfaction (expressed as a percentage of satisfied subjects). The results are shown in Table 3.

[0043] Table 3: Changes in skin elasticity and satisfaction with skin radiance after 14 and 28 days of use.

[0044] As shown in Table 3, the changes in overall skin radiance satisfaction and skin elasticity values ​​in Comparative Example 1 were not high at 14 days, 28 days, and 0 days. Comparative Example 2 (a long-lasting hydrating composition containing only sodium hyaluronate and polystyrene® spheroidized collagen, without polystyrene® microsphered PDRN) showed some changes in radiance satisfaction and skin elasticity values ​​compared to Comparative Example 1. Comparative Example 3 (a long-lasting hydrating composition containing only polystyrene® microsphered PDRN, without sodium hyaluronate or polystyrene® spheroidized collagen) showed higher changes in overall skin radiance satisfaction and skin elasticity values ​​than Comparative Example 1 and Comparative Example 2, indicating that the long-lasting hydrating composition had some effect, but was lower than Comparative Example 4. Comparative Example 4 showed the best efficacy in both overall skin radiance satisfaction and skin elasticity values, indicating that the three components in the long-lasting composition need to work synergistically to achieve the desired long-lasting hydrating effect. Therefore, the long-lasting hydrating composition, consisting of sodium hyaluronate, polystyrene® spheroidized collagen, and polystyrene® microsphered PDRN, needs to work synergistically to achieve the desired long-lasting hydrating effect. Example 1 showed an elasticity improvement of over 60% and a gloss satisfaction rate of 98% at D28, significantly outperforming any comparative example lacking a component. Comparative Example 2 (lacking collagen and hyaluronic acid) and Comparative Example 3 (lacking PDRN) both showed significantly reduced effects, demonstrating that all three components are indispensable in the long-lasting combination.

[0045] 4. Skin moisture content (MMV) Moisture content is a core evaluation indicator linking immediate optical brightening and long-lasting moisturizing effects. Its changing patterns can objectively reflect the temporal characteristics and coupling degree of their synergistic effect. Specifically: During the immediate brightening phase, the instantaneous increase in the moisture content of the stratum corneum directly alters the skin's optical properties—the refractive index and light transmittance of the hydrated stratum corneum are significantly better than in the dry state. The scattering pattern of light on the skin surface changes from diffuse reflection to directional transmission, visually presenting a "transparent and radiant" water-like glow. At the same time, the increased moisture causes the keratinocytes to swell and arrange themselves more tightly, reducing the microscopic roughness of the skin surface and improving optical smoothness, further enhancing the immediate optical brightening effect. In the long-lasting hydration phase, the sustained maintenance and deep delivery of moisture content demonstrate the synergistic efficacy of a three-tiered hydration system: sodium hyaluronate in the epidermis forms a physical water reservoir, spheroidal collagen in the dermis reconstructs the extracellular matrix gel network, and cellular-level microsphere-derived PDRN activates mitochondrial energy metabolism and endogenous hyaluronic acid synthase expression, driving the cell's self-hydration capacity. The synergy of these three factors ensures that moisture content not only reaches an immediate peak at 1 hour but also remains significantly higher than baseline levels for 48 hours and even after discontinuation, forming a closed-loop hydration maintenance mechanism of "exogenous replenishment - endogenous driving - structural locking." Therefore, the efficacy coupling degree and overall superiority of the synergistic composition of immediate optical brightening and long-lasting hydration are evaluated by dynamically monitoring the temporal changes in moisture content.

[0046] The stratum corneum moisture content of the test area was measured using a Corneometer 825 before application and at 1 h, 2 h, 4 h, 6 h, 8 h, 24 h, and 48 h after application. Each area was measured in triplicate, and the average value was taken. The results are shown in Table 4.

[0047] Table 4: Changes in skin moisture content

[0048] As shown in Table 4, the moisture content of Comparative Example 1 did not change significantly. In Comparative Example 8 (only the short-acting brightening composition), the moisture content of the short-acting component increased, but the moisture content of the long-acting component continued to decrease, failing to maintain a stable state. In Comparative Example 9 (only the long-acting hydrating composition), the moisture content of the long-acting component remained stable, but the short-acting effect was not as good as that of Comparative Example 8. In Example 1, the moisture content reached 72.3% after 1 hour and remained at 69.1% after 48 hours, which was much higher than that of the short-acting combination alone (Comparative Example 8, which decreased to 42.7% after 48 hours) or the long-acting combination alone (Comparative Example 9, which was only 52.1% after 1 hour). This demonstrates that the synergistic effect of the short-acting and long-acting components achieved "1-hour immediate hydration + 48-hour long-term moisture retention".

[0049] 5. Transdermal water loss (TEWL) This value effectively reflects the product's immediate brightening and long-lasting moisturizing effects. The principle behind this is that the skin's moisture content is closely related to its barrier function. After using the product, the TEWL value may show a positive change due to instantaneous hydration, which together confirms that the skin immediately gains a feeling of hydration and plumpness, accompanied by an improvement in visual brightness and radiance.

[0050] TEWL values ​​(unit: g / h·m) were measured using a TM300 before application and at 1 h, 2 h, 4 h, 6 h, 8 h, 24 h, and 48 h after application. 2 The lower the value, the better the skin barrier function and the less moisture loss. Each area was measured three times in parallel, and the average value was taken. The results are shown in Table 5.

[0051] Table 5: Changes in TEVL values

[0052] As shown in Table 5, the transepidermal water dispersion value (TEWL) of Comparative Example 1 did not change significantly. In Comparative Example 8 (only the short-acting brightening composition), the TWEL decreased for the short-acting version, but the TWEL for the long-acting version continued to increase, failing to maintain a stable state. In Comparative Example 9 (only the long-acting hydrating composition), the TWEL for the long-acting version remained stable, but the short-acting effect was not as good as that of Comparative Example 8. The TEWL value of Example 1 decreased from an initial 11.5 to 7.5 at 1 hour and remained stable until 48 hours. Both the short-acting and long-acting versions showed the best efficacy in terms of TWEL, indicating that a synergistic effect between the short-acting and long-acting versions is necessary to achieve both effects. This demonstrates a significant enhancement of skin barrier function and a prominent synergistic effect between immediate and long-acting effects.

[0053] 6. Security Testing Ten volunteers were in each group. After use, the volunteers were observed for any irritation reactions such as redness, swelling, heat, pain, or itching. Safety was subjectively evaluated: if one or more volunteers did not experience any of these reactions, the sample was considered unsafe; if any volunteer experienced any of these symptoms, the sample was considered unsafe; if all volunteers experienced no irritation, the sample was considered safe. Results are shown in Table 6.

[0054] Table 6: Safety Evaluation

[0055] As shown in Table 6, Comparative Example 5, which did not use Polymicro® milk phospholipid vesicles to encapsulate niacinamide, caused irritation in those with niacinamide intolerance and failed the safety verification. In contrast, Example 1, through vesicle encapsulation and high-pressure homogenization, significantly reduced the irritation of niacinamide, achieving tolerance in all sensitive skin cases.

[0056] As can be seen from the above examples and comparative examples, the composition of the present invention achieves a significant increase in L value and a significant decrease in a value and b value within 1 hour through the synergistic effect of the immediate brightening combination formed by "polytetrafluoroethylene® milk phospholipid vesicles + carnosine + sodium heparin + niacinamide" and the long-lasting water-light combination formed by "sodium hyaluronate + polyglycan® spherical collagen + polyglycan® microsphereized PDRN". It also maintains high moisture content and low TEWL value within 48 hours, while exhibiting good skin elasticity and radiance enhancement effects, and is safe and non-irritating to sensitive skin. The comparative examples, lacking any component or not employing a specific preparation process, could not achieve the same synergistic effect.

[0057] The above content is only for illustrating the technical concept of the present invention and should not be construed as limiting the scope of protection of the present invention. Any modifications made to the technical solution based on the technical concept proposed in this invention shall fall within the scope of protection of the claims of this invention.

Claims

1. A composition synergistically achieving optical brightening and long-lasting moisturization, characterized in that, By weight, it includes the following ingredients: Xanthan gum: 0.01–0.5 parts; Polytetrafluoroethylene® milk phospholipid vesicle extract 0.1–5.0 parts; niacinamide 0.5–5.0 parts; sodium heparin 0.01–0.2 parts; carnosine 0.05–0.5 parts; sodium hyaluronate 0.01–0.3 parts; polyglycin® spheroidized collagen 0.05–8.0 parts; polyglycin® microsphereized PDRN 0.05–10.0 parts; moisturizer 1–30 parts; skin feel modifier 0.01–1.5 parts; purified water to make up to 100 parts.

2. The composition synergistically achieving optical brightening and long-lasting moisturization of claim 1, wherein, The polyacrylamide® milk phospholipid vesicle extract, niacinamide, sodium heparin, and carnosine together constitute an instant brightening combination; the sodium hyaluronate, polyacrylamide® spherical collagen, and polyacrylamide® microsphered PDRN together constitute a long-lasting hydrating combination.

3. The composition synergistically achieving optical blurring and long- lasting moisturization of claim 1, wherein, The moisturizer is selected from one or more of glycerin, 1,2-pentanediol, and ethylhexylglycerin.

4. The composition synergistically achieving optical blurring and long- lasting moisturization of claim 1, wherein, The skin feel modifier is methyl glucetol polyether-20.

5. The composition synergistically achieving optical blurring and long- lasting moisturization of claim 1, wherein, The particle size of the polyglycoside® microsphere PDRN is 110~130nm; the particle size of the polyglycoside® spherical collagen is less than 120nm.

6. The composition for synergistically achieving optical brightening and long-lasting moisturizing according to claim 1, characterized in that, The Polymicro® milk phospholipid vesicle extract has a nanoscale vesicle structure with a phospholipid bilayer membrane.

7. The method for preparing the composition synergistically achieving optical whitening and long-lasting moisturization according to any one of claims 1 to 6, characterized in that, Includes the following steps: Xanthan gum, humectant and purified water were mixed, heated and stirred until homogeneous to obtain phase A; Polytetrafluoroethylene® milk phospholipid vesicle extract, nicotinamide, heparin sodium, carnosine and purified water were mixed and homogenized under high pressure to obtain phase B; Sodium hyaluronate, polyglucono-polystyrene® spherical collagen, polyglucono-polystyrene® microsphereized PDRN and purified water were mixed to obtain phase C; Cool phase A, then add phases B and C sequentially, and stir at high speed to obtain a composition that synergistically achieves optical brightening and long-lasting moisturizing.

8. The method for preparing the composition for synergistically achieving optical brightening and long-lasting moisturizing according to claim 7, characterized in that, The heating and stirring temperature is 70 ℃~85 ℃, the high-pressure homogenization temperature is 75 ℃~85 ℃, the pressure is -0.09~0.1MPa, and the homogenization is performed 5 to 10 times, each time for 1 min to 2 min.

9. The method for preparing the composition for synergistically achieving optical brightening and long-lasting moisturizing according to claim 7, characterized in that, Phase A is cooled to 30 ℃~45 ℃, and the high-speed stirring speed is 2000 rpm~5000 rpm for 15 min~30 min.

10. A skin repair formulation comprising, A composition comprising any one of claims 1 to 6 that synergistically achieves optical brightening and long-lasting moisturizing.

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