A cell culture device for convenient sampling

Abstract

This invention discloses a convenient cell culture device for sampling, relating to the field of biological cell culture technology. It includes a culture container with a culture chamber and a sampling bottle placed on a weighing sensor, forming a weighing trigger mechanism. A compression component within the container end cap applies pressure to the cell fluid within the culture chamber via a sliding extrusion slide. An opening and closing mechanism provides access for the squeezed cell fluid. A vibration component on a support base vibrates the cell fluid reaching the sampling bottle via a vibration motor. Throughout the process, a temperature control component maintains an adjustable constant-temperature culture environment for the cell fluid within the culture chamber. This invention, based on a sampling bottle placed on a weighing sensor, triggers a gravity weighing mechanism, enabling independent / synchronous sampling of cell fluid from single or multiple chambers in a sealed environment, ensuring the activity of substances within the cell fluid.

Description

Technical Field

[0001] This invention relates to the field of biological cell culture technology, and in particular to a cell culture device that facilitates sampling. Background Technology

[0002] Cell culture refers to the technique of growing, multiplying, and maintaining the main structure and function of cells under artificially created conditions similar to the in vivo environment. Cell culture is divided into primary culture and passage culture, and can also be divided into adherent culture and suspension culture according to cell growth characteristics. Cell culture technology is frequently used in new agricultural systems that utilize modern biotechnology to improve agricultural production efficiency and sustainability.

[0003] During cell culture, in order to understand the composition of substances in the cell slurry in a timely manner, it is necessary to sample the cell slurry regularly. Generally, this is done manually by using a pipette to directly insert it into the exposed cell slurry through the culture container with the lid open. This process is very easy to cause the cell slurry to be affected by the external environment, resulting in cross-contamination and interference, which reduces the activity of substances in the cell slurry. The sampling method is not rigorous enough. Summary of the Invention

[0004] The purpose of this section is to outline some aspects of embodiments of the present invention and to briefly describe some preferred embodiments. Simplifications or omissions may be made in this section, as well as in the abstract and title of this application, to avoid obscuring the purpose of these documents; however, such simplifications or omissions should not be construed as limiting the scope of the invention.

[0005] In view of the problems existing in the above and / or existing convenient sampling cell culture devices, the present invention is proposed.

[0006] Therefore, the problem to be solved by this invention is how to solve the problem that traditional sampling methods lead to the exposure of cell fluid to the outside, resulting in cross-contamination and interference, which affects the activity of substances.

[0007] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a cell culture device for convenient sampling, comprising a culture container with a culture chamber and a sampling bottle placed on a weighing sensor, forming a weighing trigger mechanism. A compression component inside the container end cap applies pressure to the cell fluid in the culture chamber through a squeezing slide in the culture chamber, and the flow channel of the opening and closing component provides opening and closing conditions for the squeezed cell fluid. A vibration component on the support base applies vibration to the cell fluid reaching the sampling bottle through a vibration motor. Throughout the process, a temperature control component creates a temperature-controlled and adjustable constant temperature culture environment for the cell fluid in the culture chamber.

[0008] As a preferred embodiment of the convenient sampling cell culture device of the present invention, the compression component further includes a servo motor embedded in the end cap of the container, and a main spherical gear is sleeved on the output shaft of the servo motor. A differential gear is arranged in a triangular equidistant shape on the outer side of the main spherical gear, and an electric push rod that rotates with the end cap of the container is fixed at the bottom of the differential gear.

[0009] As a preferred embodiment of the convenient sampling cell culture device of the present invention, wherein: the bottom of the three sets of electric push rods is fixed with a threaded short rod, and the threaded short rod is threadedly connected with a threaded sleeve that slides through the end cap of the container; the threaded sleeve and the extrusion slide are fixed by a bearing seat; and the bottom of the three sets of extrusion slides is fixed with a convex frustum that extrudes the culture chamber.

[0010] As a preferred embodiment of the convenient sampling cell culture device of the present invention, the outer side of the container end cap is provided with a warning light with digital markings, and the warning light is divided into a working light and a pending light. The three sets of weighing sensors form a gravity triggering mechanism based on the gravity sensing generated by the sampling bottle, together with the servo motor, the three sets of electric push rods and the warning light.

[0011] As a preferred embodiment of the convenient sampling cell culture device of the present invention, the opening and closing component further includes a flow equalization port circumferentially opened at the top of the flow channel cavity, and the culture cavity and the flow channel cavity are connected through the flow equalization port. The bottom of the three sets of extrusion slides is fixed with an upper linkage rod, and the top of the flow channel cavity is embedded with an upper sealing slide sleeve that slides with the upper linkage rod.

[0012] As a preferred embodiment of the convenient sampling cell culture device of the present invention, the bottom of the upper linkage rod is fixed with an inner valve plate that slides with the flow channel cavity, and the outer side of the flow channel cavity is provided with defoaming holes that are separated from the opening and closing of the inner valve plate. The outer side of the defoaming holes is connected to a flow guide head, and the bottom end of the flow guide head is connected to a sampling tube.

[0013] As a preferred embodiment of the convenient sampling cell culture device of the present invention, the homogenizing assembly further includes a lower linkage rod fixed to the bottom of the inner valve plate, and a lower sealing sleeve that slides with the lower linkage rod is embedded in the bottom of the flow channel cavity. The bottoms of the three sets of lower linkage rods penetrate the flow channel cavity and are fixed with main electrode plates. An insulating elastic pad is embedded in the support base, and a secondary electrode plate that is triggered by the compression of the main electrode plate is fixed on the three sets of insulating elastic pads.

[0014] As a preferred embodiment of the convenient sampling cell culture device of the present invention, wherein: a vibration support is fixed on the outer side of the three sets of support feet, the vibration support is embedded with a weighing sensor, and a limiting ring for preventing the sampling bottle from being blocked is fixed on the top of the vibration support, and an arc-shaped opening is provided on the outer side of the limiting ring; the three sets of vibration supports are embedded with a vibration motor that triggers the opening and closing of the main electrode plate and the auxiliary electrode plate.

[0015] As a preferred embodiment of the convenient sampling cell culture device of the present invention, the temperature control component includes a delivery channel opened in the middle of the culture container, and a conduction cavity communicating with the delivery channel is opened on the side of the culture container near the culture chamber. The three sets of conduction cavities are in a blocked state with the culture chamber, and a thin conduction wall is reserved between the conduction cavity and the culture chamber.

[0016] As a preferred embodiment of the convenient sampling cell culture device of the present invention, the culture container is respectively equipped with a small heater, a small cooler and a PLC controller, and the inner sides of the small heater and the small cooler are respectively connected to a heating valve and a cooling valve. The inner ends of the heating valve and the cooling valve are connected to a buffer head, and the top of the buffer head is connected to a conveying pipe connected to the conveying channel.

[0017] The beneficial effects of this invention are as follows: Based on the sampling bottle placed on the weighing sensor, a gravity weighing mechanism is triggered to achieve independent / synchronous sampling of cell fluid in single / multi-chamber environments under a sealed environment. This avoids the cell fluid samples being exposed to the outside and contaminated by external factors, ensuring the activity of substances in the cell fluid. At the same time, the opening and closing size of the flow channel cavity is synchronously controlled according to the amount of cell fluid sample collected. That is, the deeper the extrusion slide is pressed, the larger the channel opening of the flow channel cavity, and the more cell fluid is squeezed out of the culture chamber, which is directly proportional to the sampling. The main electrode plate and the auxiliary electrode plate successively provide trigger conditions for the vibration motor, so that the cell fluid sample is collected while the cell fluid in the sampling bottle is vibrated and homogenized to prevent the substances in the cell fluid sample from coagulating and precipitating, and to maintain a dispersed state. The overall sampling process is continuous and smooth. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the following description of the embodiments will be briefly introduced. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 An initial view of the overall structure of the cell culture device designed for convenient sampling.

[0020] Figure 2 A diagram showing the overall structure and sampling status of a cell culture device for easy sampling.

[0021] Figure 3 A front sectional view of the overall structure of the cell culture device for convenient sampling.

[0022] Figure 4An internal view of the single-chamber sampling configuration of a cell culture apparatus designed for convenient sampling.

[0023] Figure 5 An internal view of the multi-cavity sampling configuration of a cell culture device designed for convenient sampling.

[0024] Figure 6 A bottom view of the compression and opening / closing components of a cell culture apparatus for convenient sampling.

[0025] Figure 7 A top cross-sectional view of the culture container and support legs of a cell culture apparatus designed for easy sampling.

[0026] Figure 8 Exploded cross-sectional view of the culture container and temperature control components of the cell culture device for convenient sampling.

[0027] In the diagram: 1. Culture container; 2. Sealing head; 3. Culture chamber; 4. Weighing sensor; 5. Sampling bottle; 6. Container end cap; 71. Servo motor; 72. Main spur gear; 73. Differential gear; 74. Electric push rod; 75. Threaded short rod; 76. Threaded sleeve; 77. Extrusion slide; 8. Warning light; 91. Flow channel cavity; 92. Flow equalization port; 93. Upper linkage rod; 94. Inner valve plate; 95. Defoaming hole; 96. Guide head; 97. Sampling tube; 10. Upper sealing sleeve; 11. Sealing... 12. Plug end; 13. Wall-mounted opening; 141. Support foot; 142. Lower linkage rod; 143. Main electrode plate; 144. Secondary electrode plate; 145. Insulating elastic pad; 146. Vibration motor; 147. Vibration support; 148. Limiting ring; 15. Lower sealing sleeve; 161. Conveying channel; 162. Conducting cavity; 163. Small heater; 164. Small refrigerator; 165. Heating valve; 166. Cooling valve; 167. Buffer head; 168. Conveying pipe; 17. Temperature sensor. Detailed Implementation

[0028] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0029] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0030] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that is mutually exclusive with other embodiments.

[0031] Example 1, referring to Figures 1 to 8 This is the first embodiment of the present invention. This embodiment provides a cell culture device for convenient sampling, including a culture container 1 with a culture chamber 3 and an injection port on the outer side of the culture container 1 near the culture chamber 3. The injection port facilitates the injection of cell fluid into the culture chamber 3, and the injection port is sealed by a sealing head 2 to prevent dust and isolate the cell.

[0032] Culture chamber 3, which is in contact with the cell broth for a long time, is made of polystyrene with a smooth surface. Due to its chemical inertness, it does not nourish bacteria and is not easily attached by microorganisms. After TC surface treatment, it is suitable for cell adhesion. The smooth surface greatly reduces bacterial colonization. Combined with high-pressure sterilization and ultraviolet sterilization, it is not easy for miscellaneous bacteria to grow during long-term culture, thus avoiding bacterial growth.

[0033] The culture container 1 is also provided with an observation window with tempered glass on the outside of the culture chamber 3. Through the observation window with tempered glass, it is convenient to observe the culture and sampling of the cell fluid in the culture chamber 3 from the outside of the culture container 1.

[0034] The sampling bottles 5 placed on the weighing sensor 4 form a weighing trigger mechanism. According to the current sampling position, one or more sets of sampling bottles 5 are placed on one or more sets of weighing sensors 4, up to three sets. While the weighing sensor 4 weighs the gross weight of the sampling bottle 5, the weighing sensor 4 also applies the trigger condition to the weighing sensor 4 under the downward pressure of the gross weight of the sampling bottle 5.

[0035] The compression component inside the container end cap 6 applies pressure to the cell fluid in the culture chamber 3 through the squeezing slide 77 sliding in the culture chamber 3, and the sampling measure is triggered by the sampling bottle 5 placed on the weighing sensor 4. This achieves independent / synchronous sampling of cell fluid in single / multi-chamber environments in a sealed environment, avoiding exposure of the cell fluid sample to external factors and contamination of the cell fluid sample, and ensuring the activity of substances in the cell fluid.

[0036] Specifically, it also includes a servo motor 71 embedded in the container end cap 6, and a main spur gear 72 is sleeved on the output shaft of the servo motor 71. A differential gear 73 is arranged in a triangular equidistant shape on the outer side of the main spur gear 72, and an electric push rod 74 that rotates with the container end cap 6 is fixed at the bottom of the differential gear 73. The meshing stroke between the main spur gear 72 and the single / multiple sets of differential gears 73 is adjusted to the correct position by one or more electric push rods 74.

[0037] In use, based on the weighing trigger mechanism formed by the sampling bottle 5 currently placed on the weighing sensor 4, the servo motor 71 is triggered to start, and through the meshed main spur gear 72, it drives the single / multiple electric push rods 74 on the single / multiple differential gears 73 to rotate accordingly, providing stable gear-type power transmission for the sampling action of cell fluid in one / multiple culture chambers 3.

[0038] Specifically, the bottom of the three sets of electric push rods 74 is fixed with threaded short rods 75, and the threaded short rods 75 are threadedly connected with threaded sleeves 76 that slide through the container end cap 6. The threaded sleeves 76 are fixed to the extrusion slides 77 through bearing seats. The bottom of the three sets of extrusion slides 77 is fixed with convex truncated cones that press against the culture chamber 3. Through the convex truncated cones, the downward extrusion force of the extrusion slides 77 on the culture chamber 3 is increased, forcing the cell fluid under the extrusion force to flow downward quickly and preventing the cell fluid from stagnating.

[0039] In use, the single / multiple differential gears 73 that trigger rotation drive the single / multiple threaded short rods 75 to rotate forward via the single / multiple electric push rods 74. The single / multiple threaded short rods 75 drive the threaded sleeves 76 on them to move downward. Then, the single / multiple threaded sleeves 76 drive the extrusion slide 77 to slide down in the single / multiple culture chambers 3, and apply downward extrusion force to the cell fluid inside, forcing the cell fluid in the single / multiple chambers to flow downward, thus performing the sampling action.

[0040] The compression slide 77 and the culture chamber 3 are fitted with an interference fit to improve the sealing of the compression slide 77 during the sliding compression in the culture chamber 3. This prevents the cell fluid in the culture chamber 3 from overflowing the compression slide 77 and reaching the area above it, which would cause cleaning trouble. An arc-shaped chamfer is provided around the bottom edge of the compression slide 77 to prevent the compression slide 77 from sliding and getting stuck in the culture chamber 3, thus improving the smoothness of its sliding compression process.

[0041] The outer side of the container end cap 6 is equipped with a warning light 8 with digital markings. The warning light 8 is divided into a working light and a waiting light. The working light is red, indicating that cell fluid sampling is being carried out at this location. The waiting light is green, indicating that cell fluid sampling is not being carried out at this location. This allows for timely awareness of the current sampling location and status of the cell fluid. The three sets of weighing sensors 4, based on the gravity sensing generated by the sampling bottle 5, together with the servo motor 71, the three sets of electric push rods 74, and the warning light 8, form a gravity triggering mechanism. Gravity triggering can be performed individually or synchronously, depending on the number of sampling bottles 5 placed on the three sets of weighing sensors 4.

[0042] Example 2, refer to Figures 1 to 8 This is the second embodiment of the present invention, which is based on the previous embodiment.

[0043] The flow channel cavity 91 of the opening and closing component provides the channel opening and closing conditions for the squeezed cell fluid. The opening and closing size of the flow channel cavity 91 is controlled synchronously according to the amount of cell fluid sample collected. That is, the deeper the squeezing slide 77 is pressed down, the larger the channel opening of the flow channel cavity 91 is, and the more cell fluid is squeezed out of the culture chamber 3. The sampling is carried out in a direct proportional relationship.

[0044] Specifically, it also includes a flow equalization port 92 circumferentially opened at the top of the flow channel cavity 91, and the culture cavity 3 and the flow channel cavity 91 are connected through the flow equalization port 92. The bottom of the three sets of extrusion slides 77 is fixed with an upper linkage rod 93, and the top of the flow channel cavity 91 is embedded with an upper sealing sleeve 10 that slides with the upper linkage rod 93. The upper sealing sleeve 10 plays a sliding sealing role for the upper linkage rod 93 during sliding.

[0045] The bottom of the upper linkage rod 93 is fixed with an inner valve plate 94 that slides with the flow channel cavity 91. The inner valve plate 94 and the flow channel cavity 91 are in an interference fit, and the flow channel cavity 91 is slidably sealed by a rubber ring on the outer edge of the inner valve plate 94. The rubber ring is made of antibacterial rubber for cell culture. It divides the flow channel cavity 91 into an upper channel and a lower channel. The upper channel supplies cell fluid for sampling and ensures that there is no cell fluid leakage in the lower channel. The top circumference of the inner valve plate 94 is fixed with a sealing end 11 that seals the flow equalization port 92. Through the sealing end 11, when the cell fluid does not need to be sampled, the flow equalization port 92 is sealed to prevent the cell fluid in the culture chamber 3 from accidentally flowing into the upper channel of the flow channel cavity 91 and causing trouble.

[0046] Furthermore, the outer side of the flow channel cavity 91 is provided with defoaming holes 95 that are separated from the opening and closing of the inner valve plate 94. These holes are arranged in three rows from top to bottom. Through the defoaming holes 95, the flowing cell fluid is uniformly treated to avoid the cell fluid flowing too fast and generating too much foam, which would affect the sampling operation of the subsequent sampling bottle 5. In addition, the top of the inner valve plate 94 is provided with a slope. The height of the slope is designed to decrease from high to low in the direction of the defoaming holes 95, which facilitates the smooth flow of cell fluid into the opened defoaming holes 95 in the upper channel, accelerates the flow speed of the cell fluid, and prevents the cell fluid from stagnating on the inner valve plate 94.

[0047] The diameter of the equalization port 92 and the pore size of the defoaming hole 95 are both tens of times larger than the cell size, which prevents the cell material in the cell fluid from rupturing after being squeezed by the squeezing slide 77, keeping the cell material in the cell fluid intact and without damage, thus improving the effectiveness of cell fluid sampling.

[0048] The outer side of the defoaming hole 95 is connected to a flow guide head 96, and the bottom end of the flow guide head 96 is connected to a sampling tube 97 made of antibacterial rubber material for cell culture. The inner ends of the three sets of sampling tubes 97 are provided with wall-adhering openings 12 that are in contact with the inner wall of the sampling bottle 5. This forces the cell fluid in the three sets of sampling tubes 97 to flow and sample along the inner wall of the sampling bottle 5 through the wall-adhering openings 12, preventing the cell fluid from being discharged too quickly and splashing to the outside of the sampling bottle 5, causing cell fluid waste and contamination. At the same time, the wall-adhering flow method also reduces the foam agitated by the cell fluid in the sampling bottle 5, thus playing a foam suppression role.

[0049] By using a sampling tube 97 made of antibacterial rubber material specifically for cell culture, the sampling bottle 5 can be quickly passed through the sampling tube 97, forcing the wall-adhering opening 12 on the inner side of the sampling tube 97 to directly adhere to the sampling bottle 5 without tilting it. After sampling, there is no need to tilt the sampling bottle 5 to remove it, preventing cell fluid from overflowing and avoiding bacterial growth.

[0050] The flow channel 91, the flow equalization port 92, the defoaming hole 95, and the flow guide head 96 are all made of polypropylene material. Due to its resistance to high and low temperatures, acid and alkali, and its dense and smooth surface, bacteria have difficulty adhering and growing. It can be repeatedly sterilized by high temperature and high pressure, and can maintain a sterile interface even after repeated use, without releasing nutrients to nourish bacteria.

[0051] In use, when the single / multiple sets of extrusion slides 77 slide downwards in the culture chamber 3, they simultaneously drive the inner valve plates 94 on the single / multiple upper linkage rods 93 to slide down in the single / multiple flow channel cavities 91. This forces the sealing ends 11 on the single / multiple sets of inner valve plates 94 to gradually detach from the flow equalization port 92, thus opening their corresponding channels. Subsequently, the cell fluid in the single / multiple culture chambers 3, after being subjected to the downward extrusion force generated by the extrusion slides 77, is forced to flow downwards through the flow equalization port 92, which is opened, into the upper channel of the single / multiple flow channel cavities 91 formed by the inner valve plates 94.

[0052] At the same time, the inner valve plate 94, which slides down with the single or multiple upper linkage rods 93, gradually opens the three rows of defoaming holes 95 in the flow channel cavity 91. That is, the deeper the extrusion slide 77 and the inner valve plate 94 slide down, the larger the opening of the defoaming hole 95 channels becomes. Until the inner valve plate 94 touches the bottom of the lower channel of the flow channel cavity 91, all three rows of defoaming holes 95 channels are opened. At this time, the amount of cell fluid in the upper channel of the flow channel cavity 91 flows through the defoaming holes 95 and is simultaneously subjected to uniform flow defoaming treatment. Then, the sample tube 97 on the guide head 96 flows through the wall-attached opening 12 and along the inner wall of the sample bottle 5 to carry out the defoaming sampling action.

[0053] When cleaning is required inside the culture chamber 3, cleaning solution can be directly added into the culture chamber 3 through the three filling ports. The cleaning solution flows from the culture chamber 3, through the equalization port 92 and the defoaming hole 95, through the sampling tube 97 on the guide head 96, and into the water storage cup placed on the weighing sensor 4.

[0054] Example 3, referring to Figures 1 to 8 This is the third embodiment of the present invention, which is based on the first two embodiments.

[0055] The vibration unit on the support 13 applies vibration to the cell fluid in the sampling bottle 5 via the vibration motor 145. The main / sub-electrode sheet compression triggering mechanism provides the triggering condition for the vibration motor 145. While collecting cell fluid samples, the cell fluid in the sampling bottle 5 is vibrated to prevent the substances in the cell fluid sample from condensing and precipitating, and to maintain a dispersed state. The overall sampling process is continuous and smooth.

[0056] Specifically, it also includes a lower linkage rod 141 fixed to the bottom of the inner valve plate 94, and a lower sealing sleeve 15 that slides with the lower linkage rod 141 is embedded at the bottom of the flow channel cavity 91. The lower sealing sleeve 15 plays a sliding sealing role for the lower linkage rod 141 during sliding.

[0057] The components that come into direct contact with the cell fluid, such as the sampling bottle 5, the squeeze slide 77, the upper linkage rod 93, the inner valve plate 94, and the lower linkage rod 141, are made of borosilicate glass. Due to its dense and smooth surface, there are no organic precipitates, so it cannot provide carbon sources / nutrients for bacteria. It can be repeatedly sterilized by dry heat and moist heat, and it is resistant to high temperature and disinfectant immersion. It is a classic material for traditional aseptic culture.

[0058] The bottom of the three sets of lower linkage rods 141 passes through the flow channel cavity 91 and is fixed with the main electrode plate 142. The support base 13 is embedded with an insulating elastic pad 144. The three sets of insulating elastic pads 144 are fixed with auxiliary electrode plates 143 that are triggered by the compression of the main electrode plate 142. When the auxiliary electrode plate 143 is subjected to the compression force of the main electrode plate 142 on the lower linkage rod 141, the auxiliary electrode plate 143 generates downward pressure on the insulating elastic pad 144, forcing the insulating elastic pad 144 to compress downward elastically, so as to meet the compression triggering requirements of the main electrode plate 142 and the auxiliary electrode plate 143 under different stroke heights.

[0059] After the secondary electrode 143 loses the squeezing force of the primary electrode 142 on the lower linkage rod 141, the originally compressed insulating elastic pad 144 returns to its initial shape. Under the elastic potential energy force generated by the insulating elastic pad 144, the secondary electrode 143 is forced to move upward synchronously with the primary electrode 142 until the secondary electrode 143 moves to the initial highest position of the insulating elastic pad 144. At this time, the primary electrode 142 also moves upward and resets to the highest position with the lower linkage rod 141, disengaging from the secondary electrode 143 and releasing the triggering mechanism. At the same time, the insulating elastic pad 144 is designed with insulating material, which will not interfere with the triggering action of the primary electrode 142 and the secondary electrode 143, thereby improving the triggering sensitivity of the primary electrode 142 and the secondary electrode 143.

[0060] In use, the single / multiple inner valve plates 94 drive the lower linkage rods 141 to slide down in the lower channel within the single / multiple flow channel cavities 91. At the same time, the single / multiple lower linkage rods 141 also drive the main electrode plate 142 on it to move down and press against the single / multiple auxiliary electrode plates 143. This triggers the vibration motor 145 at the sampling bottle 5 to start. The single / multiple sampling bottles 5 collect cell fluid samples while the single / multiple vibration motors 145 uniformly vibrate the collected cell fluid samples, forcing the substances in the cell fluid samples to disperse, thus preventing coagulation and precipitation.

[0061] Vibration supports 146 are fixed to the outside of the three sets of support feet 13. The vibration supports 146 are embedded with the weighing sensor 4, and the vibration supports 146 are also embedded with the vibration motors 145 that trigger the opening and closing of the main electrode plate 142 and the auxiliary electrode plate 143. The vibration force generated by the single or multiple sets of vibration motors 145 is transmitted through the vibration supports 146 to the sampling bottle 5 placed on the weighing sensor 4. While the single or multiple sets of vibration motors 145 vibrate the cell fluid in the sampling bottle 5, the single or multiple sets of weighing sensors 4 weigh the cell fluid sample collected in the sampling bottle 5. The warning light 8 emits a bright red light when the working light is on throughout the process.

[0062] The weighing sensor 4, which vibrates and weighs simultaneously with the vibration motor 145, only serves as an auxiliary weighing and triggering function. If precise weighing is required, the sampled cell fluid can be sent to the laboratory for testing and weighed using a dedicated electronic scale. After precise weighing by the dedicated electronic scale, the sampled cell fluid can be subjected to closed-loop control with weight feedback.

[0063] Furthermore, the top of the vibration support 146 is fixed with a limiting ring 147 for preventing the sampling bottle 5 from being blocked. The limiting ring 147 plays a role in limiting and preventing the sampling bottle 5 from tilting during vibration, thereby improving the vibration stability of the sampling bottle 5. It also forces the wall-adhering opening 12 on the sampling tube 97 to always be in contact with the inner wall of the sampling bottle 5.

[0064] An arc-shaped opening is provided on the outside of the limiting ring 147. The arc-shaped opening facilitates the removal of the sampling bottle 5 from the limiting ring 147 on the vibration support 146 in a vertical position, preventing the sampling bottle 5 from tilting when it is removed, which would cause the cell fluid sample collected inside to spill out. After the sampling bottle 5 containing the cell fluid sample is removed, it is immediately sealed and sent for testing to reduce the entry of air.

[0065] After the single / multiple sampling bottles 5 containing cell fluid samples are removed, the weighing sensor 4 loses the downward pressure of the sampling bottle 5 and simultaneously triggers the servo motor 71, three sets of electric push rods 74 and the warning light 8 to perform a reset action. Similarly, the single / multiple squeezing slides 77 and the inner valve plate 94 slide upwards in the single / multiple culture chambers 3 and the flow channel 91, and drive the cell fluid remaining in the upper channel of the flow channel 91 to follow the inner valve plate 94 through the flow equalization port 92 that has not yet been blocked by the blocking end 11, and flow back into the culture chamber 3 until the blocking end 11 re-blocks the flow equalization port 92.

[0066] At this time, the single / multiple inner valve plates 94 also gradually close the three rows of defoaming holes 95 in the flow channel cavity 91 area, preventing the residual cell fluid in the upper channel of the flow channel cavity 91 from flowing into the guide head 96, and simultaneously driving the main electrode plate 142 at the single / multiple lower linkage rods 141 to disengage from the auxiliary electrode plate 143, releasing the trigger action of the single / multiple vibration motors 145, turning it off, stopping the vibration, until all are reset to the initial position, the warning light 8 emits a bright green light in the pending state, and waits for the next batch of cell fluid to be sampled.

[0067] Example 4, refer to Figures 1 to 8 This is the fourth embodiment of the present invention, which is based on the first three embodiments.

[0068] Throughout the process, the temperature control component creates a constant temperature culture environment with adjustable temperature for the cell solution in culture chamber 3, which is conducive to high-quality cell culture operations, avoids interference from external temperature changes on the cell solution culture environment, and also ensures the activity of substances in the cell solution.

[0069] Specifically, it includes a transport channel 161 located in the middle of the culture container 1, and a conduction cavity 162 connected to the transport channel 161 on the side of the culture container 1 near the culture chamber 3. The transport channel 161 and the conduction cavity 162 provide flow channel support for the heat / cold source. The three sets of conduction cavities 162 are in a sealed state with the culture chamber 3 to prevent the cell fluid in the culture chamber 3 from entering the conduction cavity 162, causing cell fluid waste and contamination.

[0070] Furthermore, a thin conductive wall is reserved between the conductive cavity 162 and the culture cavity 3. Through the conductive wall, the conductive thickness between the conductive cavity 162 and the culture cavity 3 is shortened, which accelerates the rapid and effective conduction of heat / cold source to the culture cavity 3, and controls the heating / cooling of the cell fluid cultured inside, thereby reducing the conduction loss of heat / cold source.

[0071] The culture container 1 is equipped with a small heater 163, a small cooler 164 and a PLC controller. The small heater 163 provides a heat source and the small cooler 164 provides a cold source. The inner sides of the small heater 163 and the small cooler 164 are respectively connected to a heating valve 165 and a cooling valve 166. The heating valve 165 and the cooling valve 166 are used to open and close the heat / cold source channels.

[0072] Temperature sensors 17 are embedded in both the heating valve 165 and the cooling valve 166 to monitor the temperature of the heat / cold source flowing through them in real time. This ensures that the temperature of the cell culture medium in the culture chamber 3 is appropriate, avoiding excessively high or low temperatures that could affect the activity of substances in the cell culture medium. This effectively creates a suitable temperature-controlled environment, which is beneficial for cell culture operations.

[0073] The inner ends of the heating valve 165 and the cooling valve 166 are connected to a buffer head 167, and the top of the buffer head 167 is connected to a conveying pipe 168 that is connected to the conveying channel 161. The heat / cold source flowing through is buffered and conveyed through the buffer head 167 and the conveying pipe 168.

[0074] In use, according to changes in the external temperature, the small heater 163 / small refrigerator 164 is turned on accordingly, and the generated heat / cold source is supplied through the buffer head 167 on the heating valve 165 / cooling valve 166 and the delivery pipe 168 into the conduction cavity 162 connected to the delivery channel 161. Based on the principle of heat / cold conduction, the heat / cold source is conducted through the conduction thin wall to the cell liquid area in the three sets of culture chambers 3, and the cell liquid is heated / cooled, forcing the cell liquid in the three sets of culture chambers 3 to be cultured in a constant temperature environment, so as to maintain the activity of the substances in the cell liquid.

[0075] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A cell culture device for convenient sampling, characterized in that: The system includes a culture container (1) with a culture chamber (3) and a sampling bottle (5) placed on a weighing sensor (4), forming a weighing trigger mechanism. The compression component inside the container end cap (6) applies pressure to the cell fluid in the culture chamber (3) through the squeezing slide (77) sliding in the culture chamber (3). The flow channel cavity (91) of the opening and closing component provides the channel opening and closing conditions for the squeezed cell fluid. The vibration component on the support (13) applies vibration to the cell fluid that reaches the sampling bottle (5) through the vibration motor (145). The temperature control component creates a temperature-controlled constant temperature culture environment for the cell fluid in the culture chamber (3) throughout the process.

2. The convenient sampling cell culture device as described in claim 1, characterized in that: The compression assembly also includes a servo motor (71) embedded in the container end cap (6), and a main spur gear (72) is sleeved on the output shaft of the servo motor (71). A differential gear (73) is arranged in a triangular equidistant shape on the outer side of the main spur gear (72), and an electric push rod (74) that rotates with the container end cap (6) is fixed at the bottom of the differential gear (73).

3. The convenient sampling cell culture device as described in claim 2, characterized in that: The bottom of the three sets of electric push rods (74) is fixed with a threaded short rod (75), and the threaded short rod (75) is threadedly connected with a threaded sleeve (76) that slides through the container end cap (6). The threaded sleeve (76) is fixed to the extrusion slide (77) through a bearing seat. The bottom of the three sets of extrusion slides (77) is fixed with a convex frustum that is extruded by the culture chamber (3).

4. The convenient sampling cell culture device as described in claim 2, characterized in that: The outer side of the container end cap (6) is equipped with a warning light (8) with digital markings, and the warning light (8) is divided into a working light and a waiting light. The three sets of weighing sensors (4) form a gravity triggering mechanism based on the gravity sensing generated by the sampling bottle (5), the servo motor (71), the three sets of electric push rods (74) and the warning light (8).

5. The convenient sampling cell culture device as described in claim 1, characterized in that: The opening and closing assembly also includes a flow equalization port (92) circumferentially opened at the top of the flow channel cavity (91), and the culture cavity (3) and the flow channel cavity (91) are connected through the flow equalization port (92). The bottom of the three sets of extrusion slides (77) is fixed with an upper linkage rod (93), and the top of the flow channel cavity (91) is fitted with an upper sealing slide sleeve (10) that slides with the upper linkage rod (93).

6. The convenient sampling cell culture device as described in claim 5, characterized in that: The bottom of the upper linkage rod (93) is fixed with an inner valve plate (94) that slides with the flow channel cavity (91), and the outer side of the flow channel cavity (91) is provided with defoaming holes (95) that are separated from the opening and closing of the inner valve plate (94). The outer side of the defoaming holes (95) is connected to a flow guide head (96), and the bottom end of the flow guide head (96) is connected to a sampling tube (97).

7. The convenient sampling cell culture device as described in claim 6, characterized in that: The vibration oscillation assembly also includes a lower linkage rod (141) fixed to the bottom of the inner valve plate (94), and a lower sealing sleeve (15) that slides with the lower linkage rod (141) is embedded in the bottom of the flow channel cavity (91). The bottom of the three sets of lower linkage rods (141) penetrates the flow channel cavity (91) and is fixed with a main electrode plate (142). An insulating elastic pad (144) is embedded in the support base (13). A secondary electrode plate (143) that is triggered by the compression of the main electrode plate (142) is fixed on the three sets of insulating elastic pads (144).

8. The convenient sampling cell culture device as described in claim 7, characterized in that: Vibration supports (146) are fixed on the outside of the three sets of support feet (13). The vibration supports (146) are embedded with the weighing sensor (4). A limiting ring (147) for blocking the sampling bottle (5) is fixed on the top of the vibration supports (146). An arc-shaped opening is provided on the outside of the limiting ring (147). The three sets of vibration supports (146) are embedded with the vibration motor (145) triggered by the main electrode plate (142) and the auxiliary electrode plate (143).

9. The convenient sampling cell culture device as described in claim 1, characterized in that: The temperature control component includes a delivery channel (161) in the middle of the culture container (1), and a conduction cavity (162) connected to the delivery channel (161) is provided on the side of the culture container (1) near the culture chamber (3). The three sets of conduction cavities (162) are in a blocked state with the culture chamber (3), and a thin conduction wall is reserved between the conduction cavity (162) and the culture chamber (3).

10. The convenient sampling cell culture device as described in claim 1, characterized in that: The culture container (1) is equipped with a small heater (163), a small refrigerator (164) and a PLC controller. The inner sides of the small heater (163) and the small refrigerator (164) are respectively connected to a heating valve (165) and a cooling valve (166). The inner ends of the heating valve (165) and the cooling valve (166) are connected to a buffer head (167), and the top of the buffer head (167) is connected to a conveying pipe (168) that is connected to the conveying channel (161).

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