Eutypa lata and methods of using the same

Abstract

This invention discloses a type of Chaetomium globosum Al-EF2 derived from shallots and its applications, belonging to the field of microbial technology. The Chaetomium globosum Al-EF2 is classified and named... Chaetomium globosum Al-EF2, deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: M 20242530, is the first endophytic Chaetomium globosum isolated from scallion leaves, providing a new direction for the exploration of Chaetomium globosum strain resources. Its natural adaptation to the vegetable growth environment makes its growth-promoting effect more targeted. The Al-EF2 strain provided by this invention achieves a triple breakthrough effect of "growth promotion + yield increase + quality improvement" on vegetable crops such as cucumbers and tomatoes. Furthermore, the preparation process of the bacterial solution is simple, and the application method is easy to promote, effectively overcoming the shortcomings of existing technologies.

Description

Technical Field

[0001] This invention relates to the field of microbial technology, specifically to a type of Chaetomium globosum Al-EF2 derived from shallots and its application in promoting growth, increasing yield and improving quality of vegetable crops. Background Technology

[0002] Endophytic fungi are a class of microorganisms that colonize plants, found in various tissues and organs such as roots, stems, leaves, flowers, and fruits. They can infect and colonize the host plant during part or all of its life cycle. Through long-term co-evolution, endophytic fungi have developed harmonious and stable symbiotic relationships with host plants, and have also formed associations with other plants outside the native host. They not only utilize the host plant's nutrients for their own growth, reproduction, and life cycle, but also provide beneficial effects on the host plant's growth and development and enhance its tolerance to environmental stresses.

[0003] Endophytic fungi can promote plant growth and development, increase crop yield, and improve product quality through multiple regulatory pathways. Some endophytic fungi can directly synthesize plant hormones to regulate plant physiological processes, such as Aspergillus niger (…). Aspergillus niger ) produces gibberellin (GA3), Trichoderma ( Trichoderma spp.) secrete indoleacetic acid (IAA), which stimulates root development and cell elongation; Awamori mold ( Aspergillus awamori Endophytic fungi such as *Penicillium* enhance plant absorption and utilization of mineral nutrients through strategies such as activating soil phosphorus and potassium nutrients and producing iron carriers. In addition, some *Penicillium* species... Penicillium spp.) and Fusarium ( Fusarium Endophytic fungi (spp.) can also increase leaf chlorophyll content and Rubisco enzyme activity, optimize photosynthetic carbon assimilation efficiency, and enhance metabolic pathways such as glycolysis and the tricarboxylic acid cycle, providing a material and energy basis for biomass accumulation. More importantly, endophytic fungi can systematically regulate the primary and secondary metabolic networks of the host plant, promoting vegetative growth while guiding the targeted enrichment of economically valuable secondary metabolites such as terpenes and alkaloids, thereby improving crop yield and quality.

[0004] Chaetomium coccidioides ( Chaetomium globosumAs a common type of endophytic fungus, *Chaetoceros* has been widely studied and applied in agricultural production due to its ability to degrade organic matter and antagonize soil-borne pathogens. Current research reports indicate that *Chaetoceros* strains from different sources can be used to promote growth and increase yield in vegetables such as cucumbers, peppers, and tomatoes. For example, *Chaetoceros* strain ND35 applied to cucumbers can achieve a yield increase of approximately 21.85%, while strain DX-THS3 applied to peppers can increase the number of fruits and the weight of individual fruits. However, existing publicly available *Chaetoceros* strains have the following technical shortcomings: First, their effects on promoting growth and increasing yield in tomatoes and cucumbers are limited, with tomato yield increases mostly in the range of 30% to 50%, and cucumber fresh weight increases of only 15% to 20%, which is insufficient to meet the actual needs of the vegetable industry for high yield and quality. Second, the application methods of some strains require complex operations such as peat substrate inoculation and foliar spraying during flowering, resulting in poor adaptability and hindering large-scale field promotion. Third, existing strains are mostly isolated from soil, sweet potatoes, peppers, and other substrates or crops, indicating insufficient exploration of the diversity of strain sources. Meanwhile, some existing methods for preparing and applying Chaetomium globulus culture have problems such as complex culture medium formulations, harsh fermentation conditions, lack of statistical verification of effect data, or low repeatability, which further limit its industrial application.

[0005] Therefore, developing a novel *Chaetoceros globosum* bacterial solution and its application scheme, which significantly promotes growth, increases yield, and improves quality, has a simple preparation process, and is convenient to apply, better meets the actual research and development needs of the current agricultural microbiology field. (Scallions) Allium fistulosum L.) is a vegetable belonging to the Amaryllidaceae family and the Allium genus. There are currently no reports on the targeted application of the endophytic chrysophyte of shallots in cucumbers and tomatoes. Summary of the Invention

[0006] The purpose of this invention is to provide a novel microorganism that has significant effects on promoting growth, increasing yield and improving quality, and whose preparation process for developing into a bio-fertilizer is simple and convenient to apply. This microorganism can be used in the cultivation of vegetable crops to promote growth, increase yield and improve quality.

[0007] To achieve the above objectives, the present invention adopts the following technical solution: Scallions Allium fistulosum L. (Allium chinense) is a widely cultivated vegetable belonging to the Amaryllidaceae family and the Allium genus. Its endophytic fungi, through long-term co-evolution, possess natural advantages adapted to the vegetable's growth environment. This invention isolates a novel *Chaetomium globosum* strain, Al-EF2, from scallion leaves. Its biological characteristics are: white colonies on CM medium, short, hairy hyphae, and good growth. The ITS sequence of this strain is shown in SEQ ID NO.1, and is similar to *Chaetomium globosum* in the GenBank database. Chaetomium globosum The similarity between the two is 100.00%.

[0008] Based on morphological and molecular identification, Al-EF2 belongs to the kingdom Fungi ( ). FungiAscomycota ( Ascomycota ), class of fecal scabies ( Sordariomycetes ), Faecaliales ( Sordariales ), Trichophyceae ( Chaetomiaceae ), genus Chaetomium ( Chaetomium ), Chaetomium coccidioides ( Chaetomium globosum Therefore, this strain was classified and named... Chaetomium globosum Al-EF2 was deposited on November 11, 2024, at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, with accession number CCTCC NO: M 20242530.

[0009] Furthermore, the culture conditions for *Chaetomium globosum* Al-EF2 are as follows: inoculated into CM medium and cultured at 28-30°C; the CM medium comprises: acid-hydrolyzed casein 3 g / L, enzyme-hydrolyzed casein 3 g / L, yeast extract 6 g / L, and sucrose 10 g / L. This invention has found that this medium can rapidly promote spore reproduction and enhance bacterial activity.

[0010] This invention has discovered that *Chaetoceros globosum* Al-EF2 spores exhibit high activity and demonstrate breakthrough effects in promoting growth, increasing yield, and improving quality in vegetable crops such as cucumbers and tomatoes. Therefore, this invention provides the application of *Chaetoceros globosum* Al-EF2 in promoting vegetable crop growth and / or improving vegetable crop yield or quality. The application is root irrigation.

[0011] Specifically, the indicators for promoting growth include: plant biomass and leaf area. The indicators for increasing yield include: single fruit weight and yield per plant. The indicators for improving quality include: soluble solids content of the fruit.

[0012] Furthermore, the vegetable crop can be, but is not limited to, cucumber and tomato. The research of this invention shows that, compared to the control group without bacterial treatment, cucumber plants treated with *Chaetomium globosa* Al-EF2 bacterial solution through root irrigation showed significantly increased overall plant fresh weight and single fruit weight. Compared to the control group without bacterial treatment, tomatoes treated with *Chaetomium globosa* Al-EF2 bacterial solution through root irrigation showed significantly increased leaf area at the third leaf position, significantly increased yield per plant, and significantly increased soluble solids content in the fruit.

[0013] Furthermore, the application includes: applying Chaetomium globosum Al-EF2 bacterial solution to the roots after transplanting vegetable crop seedlings.

[0014] Furthermore, the spore concentration in the Chaetomium globosum Al-EF2 bacterial culture is 1×10⁻⁶. 6 cells / mL - 1×10 8 spores / mL. Preferably, the spore concentration in the Chaetomium globosum Al-EF2 bacterial culture is 1×10⁻⁶ spores / mL.7 per mL.

[0015] Furthermore, the application rate of the Chaetomium globulus Al-EF2 bacterial solution is 10-30 mL / strain. Preferably, the application rate of the Chaetomium globulus Al-EF2 bacterial solution is 20 mL / strain.

[0016] Furthermore, the preparation method of the Chaetomium globosum Al-EF2 bacterial solution includes: inoculating the activated Chaetomium globosum Al-EF2 into CM liquid culture medium, culturing it at 28-30℃ and 180-220 rpm for 3-4 days, collecting the bacterial solution, filtering the bacterial solution with gauze to remove mycelial residue, and then diluting it with water to obtain the Chaetomium globosum Al-EF2 bacterial solution.

[0017] Furthermore, after transplanting vegetable seedlings, allow them to recover for 2-3 days, then apply the bacterial solution as a root drench every 2-3 days, for a total of 3-4 treatments. Specifically, after the seedlings have recovered for 2-3 days and the new leaves have stood upright, slowly pour the Al-EF2 bacterial solution around the base of the seedling stem into the substrate. Do not water excessively on the day of root drench; keep the substrate moist but not waterlogged. Water normally between treatments, avoiding excessive irrigation.

[0018] In one specific embodiment of the present invention, when the vegetable crops are cucumber 'Jinyanxin No. 7' and tomato 'Micro-Tom', the seedlings are transplanted after they have grown two cotyledons. After 2-3 days of recovery following transplanting, 20 mL of spores at a concentration of 1×10⁻⁶ per plant is injected into the substrate at the main root of the plant. 7 Al-EF2 bacterial suspension was applied at 2-day intervals for a total of 3 treatments.

[0019] Another object of the present invention is to provide a microbial preparation containing the aforementioned Chaetomium globosum Al-EF2, which is an agricultural microbial preparation for root irrigation. The active ingredient in this preparation is Chaetomium globosum Al-EF2, and agriculturally acceptable adjuvants that do not affect the activity of the bacterial solution, such as carriers and stabilizers, can be added according to field requirements.

[0020] The beneficial effects of this invention are as follows: (1) Novel strain source and filling of technical gap: The Al-EF2 strain provided by this invention is the first endophytic Chaetomium globosum isolated from scallion leaves, which provides a new direction for the exploration of Chaetomium globosum strain resources and fills the technical gap of targeted application of endophytic Chaetomium globosum in cucumber and tomato; it is naturally adapted to the vegetable growth environment and the growth promotion effect is more targeted.

[0021] (2) Breakthrough effect in promoting growth, increasing yield and improving quality, far exceeding the effects of existing reported strains: The Al-EF2 bacterial solution provided by this invention has a significantly better effect on cucumber 'Jinyanxin No. 7' and tomato 'Micro-Tom' than the existing publicly disclosed Chaetomium globosum strains. The fresh weight of cucumber plants is increased by more than 45% compared with existing strains, and the yield increase of single fruit weight far exceeds the effects of single application and compound application of existing reported strains; the yield of single tomato plants is nearly doubled, and the leaf area and soluble solids content of fruits are even better. It achieves a triple breakthrough effect of "promoting growth + increasing yield + improving quality", which can effectively improve the economic benefits of vegetable planting.

[0022] (3) The bacterial culture preparation process is simple and suitable for large-scale production: In this invention, the bacterial culture is prepared using conventional liquid CM medium. The fermentation conditions are 28-30℃ and 180-220 rpm for 3-4 days. No special medium, fermentation equipment or complex process is required. After filtration and dilution, a highly active bacterial culture can be obtained. The preparation cycle is short and the cost is low, making it suitable for large-scale production.

[0023] (4) Convenient application method and suitable for actual field application: The present invention adopts the root irrigation application method, which is simple to operate and easy to master. It does not require complex steps such as peat substrate inoculation and foliar spraying, and is suitable for the promotion and application of large-scale field planting of cucumbers and tomatoes, reducing the operation threshold for farmers.

[0024] (5) Green and environmentally friendly, in line with the needs of agricultural development: The bacterial liquid and microbial preparations of the present invention can effectively replace some chemical fertilizers and pesticides, reduce the application of chemicals in vegetable planting, alleviate soil degradation problems, and improve the safety and marketability of vegetable products. They are in line with the development direction of green agriculture and ecological agriculture and have broad application prospects. Attached Figure Description

[0025] Figure 1 The colony morphology of strain Al-EF2 cultured in solid CM medium.

[0026] Figure 2 The image shows the hyphal spore structure of strain Al-EF2 under a microscope.

[0027] Figure 3 Phylogenetic tree of strain Al-EF2.

[0028] Figure 4 Morphological diagram showing the effect of Al-EF2 bacterial solution treatment on cucumber plant growth.

[0029] Figure 5 This is a statistical graph showing the effect of Al-EF2 bacterial solution treatment on the fresh weight of cucumber plants. A t-test was used for significance analysis. * indicates... p <0.05.

[0030] Figure 6 Morphological diagram showing the effect of Al-EF2 bacterial solution treatment on cucumber fruits.

[0031] Figure 7 This is a statistical graph showing the effect of Al-EF2 bacterial treatment on the weight of a single cucumber fruit. A t-test was used for significance analysis. * indicates... p <0.05.

[0032] Figure 8 Morphological diagram showing the effect of Al-EF2 bacterial solution treatment on tomato plant growth.

[0033] Figure 9 This is a statistical graph showing the effect of Al-EF2 bacterial solution treatment on leaf area at the same leaf position (3rd leaf) of tomato plants. A t-test was used for significance analysis. * indicates... p <0.05.

[0034] Figure 10 Morphological diagram showing the effect of Al-EF2 bacterial solution treatment on red-ripe tomato fruits at the same stage.

[0035] Figure 11 This is a statistical graph showing the effect of Al-EF2 bacterial treatment on the yield of individual tomato plants. A t-test was used for significance analysis. *** indicates... p <0.001.

[0036] Figure 12 This is a statistical graph showing the effect of Al-EF2 bacterial solution treatment on the soluble solids content of tomato fruits. A t-test was used for significance analysis. * indicates... p <0.05. Detailed Implementation

[0037] The present invention will be further described below with reference to specific embodiments. These embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Any modifications or substitutions made to the methods, steps, or conditions of the present invention without departing from the spirit and essence of the invention are within the scope of the invention.

[0038] Unless otherwise specified, the experimental methods used in the following examples are conventional methods; the materials and reagents used are commercially available unless otherwise specified.

[0039] Example 1: Isolation and identification of Al-EF2, an endophytic fungus of scallions 1. Isolation, purification and morphological observation of Al-EF2 Four-season shallots collected from Anqing City, Anhui Province, were transplanted and allowed to recover after transplanting. Fresh shallot leaves were then used in experiments, and tissue isolation was performed. Specifically: Rinse the scallion leaves with clean water for 1-2 minutes, place them in a test tube containing 75% ethanol, gently shake for 1-2 minutes, rinse three times with ddH2O, place them in a test tube containing 1.5% NaClO, gently shake for 1-2 minutes, and rinse three times with sterile water to complete the sterilization process. Tissue sterilization must also be performed in a laminar flow hood, and all instruments used must be autoclaved at 121℃ for 20 minutes. After the sterilization and disinfection process, take 5 μL of the sterilized water from the final tissue cleaning and spread it onto solid malt extract agar (MEA) medium as a control.

[0040] Cut the leaves into 0.5 cm × 0.5 cm pieces and spread them evenly on solid MEA medium. Incubate at 28°C. Once mycelia have grown around the tissue, inoculate the tissue onto fresh solid MEA medium and CM medium. After 2-3 generations of purification, growth statistics and strain identification can be performed.

[0041] Solid MEA medium formulation: 15 g malt extract, 15 g soybean peptone, 15 g agar, add ddH2O to 1 L, and adjust pH to 5.6±0.2.

[0042] Solid CM culture medium formula: acid-hydrolyzed casein 3 g, enzyme-hydrolyzed casein 3 g, yeast extract 6 g, sucrose 10 g, agar 15 g, supplement with ddH2O to 1 L.

[0043] Experimental Results: A strain, named Al-EF2, was obtained through the above isolation and purification process. After two weeks of cultivation on solid CM medium, the endophytic fungus Al-EF2 showed the following results: Figure 1 As shown, the colonies are white, with short, hairy hyphae, and are growing well. The mycelial spore structure is as follows. Figure 2 As shown.

[0044] 2. Molecular biological identification of Al-EF2 Obtain 1-2 pieces of culture medium containing mycelia using a punch, remove as much of the medium as possible, place them in a grinding tube with steel balls, and immediately freeze them in liquid nitrogen before grinding. Extract genomic DNA from the ground samples using a rapid fungal genomic DNA extraction kit (purchased from Sangon Biotech (Shanghai) Co., Ltd.).

[0045] Using the crude DNA extract solution as a template, universal primers (ITS1: 5'-CTTGGCATTTAGAGGAAGTAA-3'; ITS4: 5'-GCTGCGTTCTTCATGGATGC-3') were used as upstream and downstream primers to amplify the target gene (ITS) fragment of the identified strain by PCR.

[0046] The PCR amplification system consisted of 25 μL: 12.5 μL Green Taq Mix, 1 μL forward primer ITS1, 1 μL reverse primer ITS4, 2 μL DNA template, and 8.5 μL ddH2O.

[0047] Amplification conditions: 95℃ pre-denaturation for 3 min; 95℃ denaturation for 15 s, 60℃ annealing for 15 s, 72℃ extension for 15 s, 35 cycles, 72℃ complete extension for 5 min.

[0048] After PCR products were detected by 1% agarose gel electrophoresis, they were sent to the company for sequencing. The sequencing results were compared with all ITS sequences in the NCBI GenBank database using the BLAST program, and the 10 sequences with the highest similarity were selected for sequence alignment analysis.

[0049] Experimental Results: The sequence returned from sequencing was assembled to obtain the ITS gene sequence of Al-EF2, which is 548 bp in length. The nucleotide sequence is shown in SEQ ID NO.1. The ITS gene sequence of this strain was searched for homology in the GenBank database (NCBI), and BLAST analysis was used to find the strain with the highest similarity. A phylogenetic tree was constructed, as shown below. Figure 3 The results showed that this strain was related to Chaetomium globosum (…). Chaetomium globosum The similarity was 100.00%, confirming that Al-EF2 is *Chaetoceros globosa*.

[0050] Based on the above morphological characteristics and molecular identification, Al-EF2 belongs to the kingdom Fungi ( ). Fungi Ascomycota ( Ascomycota ), class of fecal scabies ( Sordariomycetes ), Faecaliales ( Sordariales ), Trichophyceae ( Chaetomiaceae ), genus Chaetomium ( Chaetomium ), Chaetomium coccidioides ( Chaetomium globosum Therefore, Al-EF2 is named... Chaetomium globosum Al-EF2.

[0051] Al-EF2 was deposited at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, on November 11, 2024, with accession number CCTCC NO: M 20242530; and was identified as viable on November 18, 2024.

[0052] Example 2: Effects of Al-EF2 root irrigation on cucumber growth promotion and yield increase 1. Experimental Materials Vegetable crop tested: cucumber 'Jinyanxin No. 7'.

[0053] Test strain: Al-EF2, an endophytic fungus of scallions.

[0054] 2. Experimental Methods The cucumber variety 'Jinyanxin No. 7' was sown in 72-cell trays. After the seedlings grew two cotyledons, seedlings of uniform size were selected and transplanted into seedling pots with a diameter of 10 cm, one seedling per pot. The cultivation substrate used was sterilized by high-pressure steam at 121℃ for 20 min. Each treatment was replicated with at least 10 seedlings.

[0055] Take a freshly cultured mycelial cake (Example 1) using a punch and place it in 300-500 mL of CM liquid culture medium. Incubate at 28°C and 200 rpm for 3-4 days, then collect the mycelial solution. Filter the solution through gauze to remove mycelial residue, then dilute with ddH2O to the desired concentration. The spore concentration is 1×10⁻⁶. 7 The formula for liquid CM medium is as follows: 3g acid-hydrolyzed casein, 3g enzymatically hydrolyzed casein, 6g yeast extract, 10g sucrose, ddH2O added to 1L, and autoclaved at 121℃ for 20 min.

[0056] Two treatments were set up for each plant: a control group treated with sterile liquid CM culture medium and a group treated with Al-EF2 bacterial solution for root irrigation. During treatment, 20 mL of sterile CM culture medium or bacterial solution was injected into the substrate at the main root of each plant using a syringe.

[0057] After transplanting, the seedlings were allowed to recover for 2-3 days before being drenched in bacterial solution. This treatment was repeated every 2 days for a total of 3 times. After treatment, the overall growth of the plants was observed regularly, and the flowering and fruiting periods were recorded. Once the fruits were fully mature, the fruit yield and quality indicators were statistically analyzed. When differences in growth were observed, the root length of the cucumber plants was measured with a tape measure, and the fresh weight of the plants and the quality of the fruits were measured with a balance.

[0058] 3. Experimental Results Effects on promoting growth and increasing yield: such as Figure 4 and Figure 5 As shown, compared with the control group, the cucumber plants in the Al-EF2 bacterial solution treatment group grew more robustly, and the fresh weight of the whole plant increased by 29.09% compared with the control group. p <0.05).

[0059] like Figure 6 and Figure 7 As shown, compared with the control group, the cucumbers in the Al-EF2 bacterial solution treatment group were larger and fuller, with a single fruit weight increase of 35.56% ( p <0.05).

[0060] The above results indicate that root irrigation with Al-EF2 bacterial solution has a significant effect on promoting growth and increasing yield in cucumbers.

[0061] Example 3: Effects of Al-EF2 root irrigation on growth promotion and yield increase in tomatoes 1. Experimental Materials The tested vegetable crop was tomato 'Micro-Tom'.

[0062] Test strain: Al-EF2, an endophytic fungus of scallions.

[0063] 2. Experimental Methods Tomato 'Micro-Tom' was sown in 72-cell trays. After the seedlings had grown two cotyledons, seedlings of the same size were selected and transplanted into seedling pots with a diameter of 10 cm, one seedling per pot. The cultivation substrate used was sterilized by high-pressure steam at 121℃ for 20 min. Each treatment was replicated with at least 10 seedlings.

[0064] Take a freshly cultured mycelial cake (Example 1) using a punch and place it in 300-500 mL of CM liquid culture medium. Incubate at 28°C and 200 rpm for 3-4 days, then collect the mycelial solution. Filter the solution through gauze first, then dilute it with ddH2O to the required concentration, with a spore concentration of 1×10⁻⁶. 7 per mL.

[0065] Two treatments were set up for each plant: a control group treated with sterile liquid CM culture medium and a group treated with Al-EF2 bacterial solution for root irrigation. During treatment, 20 mL of sterile CM culture medium or bacterial solution was injected into the main root of each plant using a syringe.

[0066] Two to three days after transplanting, the seedlings were treated with a bacterial solution for root irrigation, repeated every two days for a total of three treatments. After treatment, the overall growth of the plants was regularly observed and relevant growth indicators were recorded. The flowering and fruiting periods were also recorded. Fruit yield and quality indicators were calculated after the fruits were fully mature. ImageJ software was used to calculate the leaf area of ​​the tomato plants, a balance was used to measure the fresh weight of the plants and the weight of the fruits, and an Aituo PAL-1 saccharimeter was used to determine the soluble solids content of the tomato fruits.

[0067] 3. Experimental Results Effects of "Micro-Tom" on promoting growth, increasing yield, and improving quality in tomatoes: such as Figure 8 and Figure 9 As shown, compared with the control group, the tomato plants in the Al-EF2 bacterial solution treatment group were more robust, and the leaf area of ​​the third leaf position increased by 27.79% compared with the control group. p <0.05).

[0068] like Figure 10 and Figure 11 As shown, compared with the control group, the Al-EF2 bacterial solution treatment group produced more fruits per plant, larger fruits, and a 72.58% increase in yield per plant compared with the control group.p <0.001).

[0069] like Figure 12 As shown, compared with the control group, the soluble solids content of tomato fruits in the Al-EF2 bacterial solution treatment group increased by 12.92% ( p <0.05).

[0070] The above results indicate that Al-EF2 bacterial solution root irrigation treatment achieves a triple effect on promoting growth, increasing yield, and improving quality in tomatoes.

[0071] All the above experiments included a blank control in sterile liquid CM culture medium, with at least 10 replicates for each treatment. Statistical analysis was performed using the t-test, which clarified key technical indicators such as vegetable varieties, cultivation conditions, bacterial concentration, and application parameters. The experimental design is reproducible and the effect data is verifiable, providing a solid scientific foundation for subsequent industrial applications.

[0072] The above experiments have verified that the *Chaetomium globosum* Al-EF2 provided by this invention has a breakthrough effect on promoting growth, increasing yield, and improving quality in cucumbers and tomatoes. Furthermore, the preparation process of the bacterial solution is simple, and the application method is easy to promote, effectively overcoming the shortcomings of existing technologies. The bacterial agent provided by this invention can effectively replace some chemical fertilizers and pesticides, reducing the application of chemicals in vegetable cultivation, alleviating soil degradation problems, and improving the safety and marketability of vegetable products. It aligns with the development direction of green agriculture and ecological agriculture, and has broad application prospects.

Claims

1. A Chaetomium globosum Al-EF2, characterized by, The classification name of the *Chaetomium globosum* Al-EF2 is... Chaetomium globosum Al-EF2 is deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: M 20242530.

2. The Chaetomium globosum Al-EF2 of claim 1, wherein, The culture conditions for Chaetomium globosum Al-EF2 are as follows: inoculated into CM medium and cultured at 28-30℃; the composition of the CM medium includes: acid-hydrolyzed casein 3 g / L, enzyme-hydrolyzed casein 3 g / L, yeast extract 6 g / L, and sucrose 10 g / L.

3. The application of Chaetomium globosum Al-EF2 as described in claim 1 or 2 in promoting vegetable crop growth and / or improving vegetable crop yield or quality.

4. Use according to claim 3, wherein the compound is ###0002### The vegetable crop mentioned is either cucumber or tomato.

5. Use according to claim 3 or 4, wherein the compound is ###0002### The application includes: applying Chaetomium globosum Al-EF2 bacterial solution to the roots after transplanting vegetable crop seedlings.

6. The use according to claim 5, wherein the compound is ###0002### The spore concentration in the *Chaetoceros globosum* Al-EF2 bacterial culture was 1×10⁻⁶. 6 cells / mL - 1×10 8 per mL.

7. Use according to claim 6, wherein The application rate of the Chaetomium globosum Al-EF2 bacterial solution is 10-30 mL / strain.

8. The use according to claim 5, wherein the compound is ###0002### The preparation method of the Chaetomium globosum Al-EF2 bacterial solution includes: inoculating the activated Chaetomium globosum Al-EF2 into CM liquid culture medium, culturing it at 28-30℃ and 180-220 rpm for 3-4 days, collecting the bacterial solution, filtering the bacterial solution with gauze to remove mycelial residue, and then diluting it with water to obtain the Chaetomium globosum Al-EF2 bacterial solution.

9. The use according to claim 5, wherein the compound is ###0005### After transplanting, vegetable seedlings should be allowed to recover for 2-3 days. Then, the roots should be drenched with bacterial solution every 2-3 days for a total of 3-4 treatments.

10. A microbial preparation, characterized in that, The preparation contains Chaetomium globosum Al-EF2 as described in claim 1 or 2, and the preparation is an agricultural microbial preparation for root irrigation.

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