A highly dispersible powder and its preparation method

Abstract

This invention discloses a highly dispersible powder and its preparation method, belonging to the field of microbial powder technology. The powder mainly consists of one or more of the spore powders of *Trichoderma harzianum*, *Penicillium barometz*, *Aspergillus niger*, and *Paecilomyces lilacinus*, along with a dispersing agent and a carrier. Through improved additives, this invention significantly enhances the uniformity and stability of spore dispersion in water, eliminates spore aggregation and occlusion problems, and significantly improves the detection quantity and accuracy of viable bacteria. The carrier allows for precise dilution of the spore powder, enabling flexible preparation of microbial agents with different viable bacteria concentrations, suitable for various agricultural applications. This product exhibits excellent dispersibility, a simple process, and wide applicability, showing promising prospects for agricultural applications.

Description

Technical Field

[0001] This invention relates to the field of microbial preparation processing technology, specifically to a multi-strain composite highly dispersed microbial powder and its preparation method. Background Technology

[0002] A compound microbial agent containing Trichoderma harzianum, Penicillium barometz, Aspergillus niger, and Paecilomyces lilacinus is widely used in facility agriculture and field planting due to its multiple advantages, including broad-spectrum antibacterial activity, soil improvement, and growth promotion. The uniformity of water dispersion of the microbial powder is a core indicator determining the effectiveness of the agent and the accuracy of viable bacteria detection.

[0003] Currently available multi-strain microbial powders generally suffer from severe dispersion defects: the spore powder particles are uneven in size and easily aggregate, failing to disperse autonomously upon entering water. Large numbers of spores clump together and settle, leading not only to uneven distribution of effective bacteria and insufficient local efficacy during field application, but also to undercounted results and distorted data during viable bacteria detection due to spore aggregation obscuring the count, thus failing to accurately reflect product quality. Furthermore, traditional powders lack the flexibility to adjust viable bacteria content, making them unsuitable for different standardized production needs and severely hindering the standardization and industrialization of microbial agents. Therefore, developing a highly dispersed, accurately detectable, and adjustable viable bacteria powder is of significant practical importance. Summary of the Invention

[0004] The purpose of this invention is to solve the technical problems of poor dispersibility, severe spore aggregation, low accuracy of viable bacteria detection, and inability to control viable bacteria content in existing multi-strain microbial powders. This invention provides a highly dispersible powder and its preparation method, which effectively improves the uniformity of spore dispersion in water, accurately increases the number of viable bacteria detected, and enables flexible control of the viable bacteria content in the product.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: A highly dispersible powder is composed of the following components: microbial spore powder, dispersant, and carrier; wherein the microbial spore powder is one or more of Trichoderma harzianum spore powder, Penicillium baicalensis spore powder, Aspergillus niger spore powder, and Paecilomyces lilacinus spore powder, and a single strain or any combination of strains can be flexibly selected according to actual prevention and control needs.

[0006] The dispersant is one or more of the following: sodium polyacrylate, sodium salt of maleic anhydride copolymer, ammonium salt of naphthalene sulfonic acid condensate, Span-40, Span-60, Span-65, polyvinylpyrrolidone, polymaleic anhydride, alkylphenol formaldehyde condensate sulfonate, sodium alginate, phosphate salt dispersant, styrene-maleic acid copolymer, and aminosulfonic acid condensate. The addition amount is 1~10 wt%. The high-efficiency dispersant can effectively break the agglomeration structure of microbial spore powder, reduce the surface binding force of spores, promote the rapid, uniform, and stable dispersion of spores in water, and prevent spore aggregation. At the same time, it can eliminate the problem of live bacteria masking caused by spore aggregation, significantly reduce the number of live bacteria missed detections, significantly improve the detection quantity and detection accuracy, and ensure the authenticity and reliability of detection data.

[0007] The carrier is one or more of glucose, sodium chloride, gluten powder, soybean powder, protein powder, diatomaceous earth, soybean protein powder, mica powder, calcite powder, starch, calcium carbonate, and rice husk powder. The carrier has stable physicochemical properties and good dilution uniformity, which can accurately gradient dilute the compound microbial spore powder. It can prepare highly dispersed powders with different effective live bacteria concentrations as needed, and adapt to the production needs of standardized and serialized products.

[0008] The present invention also provides a method for preparing the above-mentioned highly dispersed powder, comprising the following steps: premixing the compound microbial spore powder, dispersant and carrier in proportions, stirring at a speed of 20~500 r / min for a stirring time of 10~40 min to achieve initial dispersion of the powder, followed by ultra-fine pulverization, and finally sieving to remove impurities, thereby obtaining a highly dispersed microbial powder product with excellent dispersion performance and uniform particle size.

[0009] Advantages of the product of this invention: 1. This invention adds a special dispersing agent, which completely solves the problems of spore aggregation and uneven dispersion in traditional multi-strain powders, greatly improves the dispersion stability of the powder in water, ensures uniform distribution of the microbial community after field application, and fully exerts the functions of soil improvement and disease control, thereby improving the field application effect.

[0010] 2. The dispersion system of the present invention can effectively expose aggregated spores, avoid the masking and missed detection of live bacteria, significantly improve the accuracy of live bacteria detection and the effective count, solve the pain points of distorted data and difficulty in quality control of bacterial agent detection in the industry, and improve the standardization of products.

[0011] 3. This invention, combined with a controllable dilution carrier system, can flexibly produce a series of products with different live bacteria contents, adapting to the application needs of different crops and different soil conditions, and the products have strong versatility; the preparation process is simple and controllable, the powder particle size is uniform, the product has strong stability, and it is suitable for industrial mass production. Attached Figure Description

[0012] To make the objectives, technical solutions, and beneficial effects of this invention clearer, the following drawings are provided for illustration: Figure 1 This is a comparison of the number of viable bacteria detected in highly dispersed powders (Examples 1-5) and unmodified powders (Comparative Examples 1-5). Detailed Implementation

[0013] The technical effects of a highly dispersible powder and its preparation method according to the present invention will be further explained below with reference to specific embodiments. However, the specific implementation methods mentioned in these embodiments are only examples of the technical solutions of the present invention and are not intended to limit the scope of implementation of the present invention. Any improvements or substitutions based on the above principles and on the basis of the present invention should be within the protection scope of the present invention.

[0014] Example 1 High-quality fungal agent powder is prepared using highly active Trichoderma harzianum spore powder (50 billion / g) as the core raw material, combined with a dispersant (Span-65) and an inert carrier (glucose). The raw materials are precisely proportioned according to a mass ratio of 100:15:400. During the production process, the carrier, dispersant, and Trichoderma harzianum spore powder are added sequentially to a closed mixing device to avoid dust generation and loss of active ingredients. After the materials are added, the stirring speed is set to 200 rpm and stirred continuously for 30 minutes to ensure thorough mixing and uniform initial mixing. The mixed material is then subjected to ultra-fine grinding and grading sieving using an 80-mesh standard sieve to effectively filter out lumps and large particles, further refining the powder particle size. The final product is a highly dispersible Trichoderma harzianum powder with uniform particle size, fine texture, and stable performance.

[0015] Example 2 High-quality fungal agent powder is prepared using highly active Penicillium harzianum spore powder (80 billion / g) as the core raw material, combined with a dispersant (Span-65) and an inert carrier (glucose). The raw materials are precisely proportioned according to a mass ratio of 100:24:700. During the production process, the carrier, dispersant, and Trichoderma harzianum spore powder are added sequentially to a closed mixing device to avoid dust generation and loss of active ingredients. After the materials are added, the stirring speed is set to 200 rpm and stirred continuously for 30 minutes to ensure thorough mixing and uniform initial mixing. The mixed material is then subjected to ultra-fine grinding and grading sieving using an 80-mesh standard sieve to effectively filter out lumps and large particles, further refining the powder particle size. The final product is a highly dispersible Trichoderma harzianum powder with uniform particle size, fine texture, and stable performance.

[0016] Example 3 High-quality fungal agent powder is prepared using highly active Paecilomyces lilacinus spore powder (100 billion / g) as the core raw material, combined with a dispersant (Span-65) and an inert carrier (glucose). The raw materials are precisely proportioned according to a mass ratio of 100:30:900. During the production process, the carrier, dispersant, and Trichoderma harzianum spore powder are added sequentially to a closed mixing device to avoid dust generation and loss of active ingredients. After the materials are added, the stirring speed is set to 200 rpm and stirred continuously for 30 minutes to ensure thorough mixing and uniform initial mixing. The mixed material is then subjected to ultra-fine grinding and grading sieving using an 80-mesh standard sieve to effectively filter out lumps and large particles, further refining the powder particle size. The final product is a highly dispersible Trichoderma harzianum powder with uniform particle size, fine texture, and stable performance.

[0017] Example 4 High-quality fungal agent powder is prepared using highly active Aspergillus niger spore powder (10 billion / g) as the core raw material, combined with a dispersant (Span-65) and an inert carrier (glucose). The raw materials are precisely proportioned according to a mass ratio of 100:3:0. During the production process, the carrier, dispersant, and Trichoderma harzianum spore powder are added sequentially to a closed mixing device to avoid dust generation and loss of active ingredients. After the materials are added, the stirring speed is set to 200 rpm and stirred continuously for 30 minutes to ensure thorough mixing and uniform initial mixing. The mixed material is then subjected to ultra-fine grinding and grading sieving using an 80-mesh standard sieve to effectively filter out lumps and large particles, further refining the powder particle size. The final product is a highly dispersible Trichoderma harzianum powder with uniform particle size, fine texture, and stable performance.

[0018] Example 5 High-quality fungal agent powder is prepared using highly active *Trichoderma harzianum* (50 billion / g) and *Penicillium baicalensis* (80 billion / g) spore powder as the core raw materials, combined with a dispersant (Span-65) and an inert carrier (glucose). The *Trichoderma harzianum* spore powder, *Penicillium baicalensis* spore powder, dispersant, and carrier are precisely proportioned in a mass ratio of 50:50:19.5:550. During production, the carrier, dispersant, *Trichoderma harzianum* spore powder, and *Penicillium baicalensis* spore powder are added sequentially to a closed mixing device to avoid raw material dust and loss of active ingredients. After feeding, the equipment stirring speed is set to 200 rpm and stirred continuously for 30 minutes to ensure thorough mixing and uniform initial mixing of the various raw materials. The mixed material is then subjected to ultra-fine grinding and grading sieving, using an 80-mesh standard sieve to effectively filter out lumps and large particles, further refining the powder particle size. The final product is a highly dispersible powder of Trichoderma harzianum with uniform particle size, fine texture, and stable performance.

[0019] Comparative Example 1 High-quality fungal agent powder is prepared using highly active Trichoderma harzianum spore powder (50 billion / g) as the core raw material, combined with an inert carrier (glucose). The raw materials are precisely proportioned at a mass ratio of 100:400. During production, the carrier and Trichoderma harzianum spore powder are added sequentially to a closed mixing device to avoid dust generation and loss of active ingredients. After feeding, the equipment is set to a stirring speed of 200 rpm and continuously stirred for 30 minutes to ensure thorough mixing and uniform initial mixing. The mixed material then undergoes ultra-fine grinding and grading sieving, using an 80-mesh standard sieve to effectively filter out lumps and large particles, further refining the powder particle size. The final product is a Trichoderma harzianum powder with uniform particle size, fine texture, and stable performance.

[0020] Comparative Example 2 High-quality fungal agent powder is prepared using highly active Penicillium baicaleum spore powder (80 billion / g) as the core raw material, combined with an inert carrier (glucose). The raw materials are precisely proportioned at a mass ratio of 100:700. During the production process, the carrier and Penicillium baicaleum spore powder are added sequentially to a closed mixing device to avoid dust generation and loss of active ingredients. After the materials are added, the stirring speed is set to 200 rpm and stirred continuously for 30 minutes to ensure thorough mixing and uniform initial mixing. The mixed material is then subjected to ultra-fine grinding and grading sieving, using an 80-mesh standard sieve to effectively filter out lumps and large particles, further refining the powder particle size. The final product is a Trichoderma harzianum powder with uniform particle size, fine texture, and stable performance.

[0021] Comparative Example 3 High-quality fungal agent powder is prepared using highly active Paecilomyces lilacinus spore powder (100 billion / g) as the core raw material, combined with an inert carrier (glucose). The raw materials are precisely proportioned at a mass ratio of 100:900. During the production process, the carrier and Paecilomyces lilacinus spore powder are added sequentially to a closed mixing device to avoid dust generation and loss of active ingredients. After the materials are added, the stirring speed is set to 200 rpm and stirred continuously for 30 minutes to ensure thorough mixing and uniform initial mixing. The mixed material is then subjected to ultra-fine grinding and grading sieving, using an 80-mesh standard sieve to effectively filter out lumps and large particles, further refining the powder particle size. The final product is a Trichoderma harzianum powder with uniform particle size, fine texture, and stable performance.

[0022] Comparative Example 4 High-quality fungal agent powder is prepared using highly active Aspergillus niger spore powder (10 billion / g) as the core raw material, combined with an inert carrier (glucose). The raw materials are precisely proportioned at a 100:0 mass ratio. During production, the carrier and Aspergillus niger spore powder are added sequentially to a closed mixing device to avoid dust generation and loss of active ingredients. After feeding, the equipment is set to a stirring speed of 200 rpm and continuously stirred for 30 minutes to ensure thorough mixing and uniform initial mixing. The mixed material is then subjected to ultra-fine grinding and grading sieving using an 80-mesh standard sieve to effectively filter out lumps and large particles, further refining the powder particle size. The final product is a Trichoderma harzianum powder with uniform particle size, fine texture, and stable performance.

[0023] Comparative Example 5 High-quality fungal agent powder is prepared using highly active *Trichoderma harzianum* spore powder (50 billion / g) and *Penicillium baicalensis* spore powder (80 billion / g) as core raw materials, combined with an inert carrier (glucose). The *Trichoderma harzianum* spore powder, *Penicillium baicalensis* spore powder, and carrier are precisely proportioned in a 50:50:550 mass ratio. During production, the carrier, *Trichoderma harzianum* spore powder, and *Penicillium baicalensis* spore powder are added sequentially to a closed mixing device to avoid raw material dust and loss of active ingredients. After feeding, the equipment is set to a stirring speed of 200 rpm and continuously stirred for 30 minutes to ensure thorough mixing and uniform initial mixing. The mixed material is then subjected to ultra-fine grinding and grading sieving, using an 80-mesh standard sieve to effectively filter out lumps and large particles, further refining the powder particle size. The final product is a *Trichoderma harzianum* powder with uniform particle size, fine texture, and stable performance.

[0024] The actual number of viable bacteria in the wettable, highly dispersible microbial powders prepared in Examples 1-5 was tested, and the actual number of viable bacteria was determined according to GB 20287-2006 standard.

[0025] Table 1

[0026] As shown in Table 1, the actual number of viable bacteria in the highly dispersed microbial powders prepared in Examples 1-5 of this invention is closer to the theoretical value and is much higher than the actual number of viable bacteria in the microbial agents prepared in Comparative Examples 1-5.

[0027] The above description is merely a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A highly dispersible powder, characterized in that, It is composed of the following components: microbial spore powder, dispersant, and carrier; wherein the microbial spore powder is one or more of Trichoderma harzianum spore powder, Penicillium baicalensis spore powder, Aspergillus niger spore powder, and Paecilomyces lilacinus spore powder.

2. The highly dispersible powder according to claim 1, characterized in that, The dispersant is one or more of the following: sodium polyacrylate, sodium salt of maleic anhydride copolymer, ammonium salt of naphthalene sulfonic acid condensate, Span-40, Span-60, Span-65, polyvinylpyrrolidone, polymaleic anhydride, alkylphenol formaldehyde condensate sulfonate, sodium alginate, phosphate ester dispersant, styrene-maleic acid copolymer, and aminosulfonic acid condensate. The amount of the dispersant added is 1 to 10 wt.

3. The highly dispersible powder according to claim 1, characterized in that, The carrier is one or more of glucose, sodium chloride, gluten powder, soybean powder, protein powder, diatomaceous earth, soybean protein powder, mica powder, calcite powder, starch, calcium carbonate, and rice husk powder. The carrier can precisely dilute the compound microbial spore powder to adapt to the large-scale production of products with different live bacteria contents.

4. The highly dispersible powder according to claim 1, characterized in that, The compound microbial spore powder contains four types of spore powders in independent proportions and can be arbitrarily combined, with the content of each spore powder adjustable according to the needs of prevention and control.

5. A method for preparing a highly dispersible powder according to any one of claims 1 to 4, characterized in that, The process includes the following steps: premixing the compound microbial spore powder, dispersant, and carrier in appropriate proportions, dispersing them at high speed, then pulverizing them into ultrafine powder, and finally sieving them to obtain a highly dispersible microbial powder.

6. The preparation method according to claim 5, characterized in that, The stirring speed is 20~500 r / min, and the stirring time is 10~40 min.

7. The application of the highly dispersible powder according to any one of claims 1 to 4 or the powder prepared by the preparation method according to any one of claims 5 to 6 in improving the accuracy of microbial viable bacteria detection, soil improvement, and control of soil-borne crop diseases.

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