A herbicidal composition comprising oxazolidinone, oxazolidinone and isoxaflutole and its application.

Abstract

This invention provides a herbicidal composition comprising oxazolidinone, oxazolidinone, and isoxaflutole, wherein the active ingredients are in the following mass ratio: oxazolidinone, oxazolidinone, and isoxaflutole in a mass ratio of 9-13:1:6-10. This herbicidal composition can be used for the control of grass weeds, broadleaf weeds, and sedge weeds. The composition has a significant synergistic effect, can effectively reduce the dosage of active ingredients, and is observed to be safe for crops in field studies.

Description

Technical Field

[0001] This invention relates to the field of pesticides, and in particular to a herbicidal composition comprising oxazolidinone, oxazolidinone and isoxaflutole, and its application. Background Technology

[0002] Oxazosyl-methyl is an aryloxyphenoxypropionic acid-based systemic herbicide for controlling annual grass weeds. Its mechanism of action is as an acetyl-CoA carboxylase (ACCase) inhibitor, which inhibits the synthesis of fatty acids in plants. It is used for foliar application to control various grass weeds such as barnyard grass and Echinochloa crus-galli. It is absorbed through the stems and leaves and translocated to the growing point via the vascular bundles to achieve weed control. Oxacillin is a systemic herbicide with low active ingredient dosage, a long suitable application period, good diffusion, and a long residual effect. It has high selectivity safety for rice and can control various weeds such as barnyard grass, Stellaria media, Echinochloa crus-galli, and Cyperus difformis. Isoxaben is a heterocyclic selective pre-emergence herbicide that controls chlorophyll synthesis in sensitive plants, causing plant death in a short period. It is effective in controlling annual weeds such as barnyard grass, Echinochloa crus-galli, Cyperus difformis, Cyperus rotundus, and Chamaedorea javanica.

[0003] Patent document CN201410598513 discloses a herbicide composition comprising a first active ingredient, oxadiazon, and a second active ingredient, wherein the second active ingredient is selected from the group consisting of oxadiazon, bispyribac-sodium, dicyclosulfuron, isoxaflutole, metsulfuron-methyl, and tetrazolesulfuron. Patent document CN201710223091 discloses a herbicide composition containing oxadiazon and oxadiazon, comprising the active ingredients oxadiazon and oxadiazon in a weight ratio of (19:1) to (1:19). There are no prior art reports of herbicide compositions combining oxadiazon, oxadiazon, and isoxaflutole. Summary of the Invention

[0004] The purpose of this invention is to provide a herbicidal composition comprising oxazolidinone, oxazolidinone, and isoxaflutole. The specific formulation is as follows: A herbicidal composition comprising oxazolidinone, oxazolidinone and isoxaflutole, characterized in that it comprises active ingredients in the following mass ratio: oxazolidinone, oxazolidinone and isoxaflutole in a mass ratio of 5-15:1:4-14.

[0005] Preferably, the mass ratio of oxazolidinyl acetamiprid, oxazolidinyl acetamiprid, and isoxaflutole is 9-13:1:6-10.

[0006] The preferred mass ratio of oxazolidinyl acetamiprid, oxazolidinyl acetamiprid, and isoxaflutole is 11:1:8.

[0007] The herbicidal composition of the present invention contains 1-75% of the active ingredient and includes adjuvant ingredients usable in pesticides. Preferably, the percentage of the active ingredient is 5-60%. The additives contain one or more of the following: dispersants, wetting agents, emulsifiers, thickeners, binders, disintegrants, preservatives, solubilizers, antifreeze agents, fillers, and solvents.

[0008] The formulation of the herbicidal composition can be emulsifiable concentrate, suspension concentrate, dispersible oil suspension, wettable powder, or water-dispersible granule.

[0009] The herbicidal composition of this invention is used for controlling weeds in crop fields. Preferably, the crop is rice; the weeds are grasses, broadleaf weeds, and sedges. Specifically, the weeds are barnyard grass, Echinochloa crus-galli, Stellaria media, Cyperus difformis, Cyperus rotundus, and Monochoria vaginalis.

[0010] Beneficial technical effects When the mass ratios of oxazolidinone, oxazolidinone, and isoxaflutole were 13:1:6, 11:1:8, and 9:1:10, they showed significant synergistic effects on barnyardgrass.

[0011] When oxazolidinyl, oxazolidinyl, and isoxaflutole technical grade are used in combination, the efficacy of the herbicidal composition is significantly improved. Compared with binary compound compositions, it shows a significant synergistic effect, which can effectively reduce the dosage of active ingredients, reduce the development of herbicide resistance in weeds, and reduce environmental pollution. Detailed Implementation

[0012] Example 1: Indoor bioassay of herbicide composition against barnyard grass Test reagents: oxazolidinyl, oxazolidinyl, and isoxaflutole; all of the above components were purchased from the market.

[0013] Test target: barnyard grass, collected in the field, for later use.

[0014] Cultivation conditions: Select several plastic pots, add sterilized soil, and use bottom irrigation to fully moisten the soil. Sow barnyard grass seeds evenly on the soil surface, cover with an appropriate amount of sifted fine soil, and then move to a greenhouse for conventional cultivation. Water the pots using bottom irrigation. After a period of cultivation, thin the seedlings to 25 plants per pot. When the barnyard grass has 3-4 leaves, spray it with water.

[0015] Chemical treatment: Accurately weigh the test reagent, add a small amount of emulsifier Tween 80 and N,N-dimethylformamide to dissolve, and then prepare a 1% stock solution with a 0.1% Tween 80 aqueous solution. Dilute the stock solution with a 0.1% Tween 80 aqueous solution. Set 7 doses using the double-dilution method for each reagent. Perform foliar spraying according to the designed dosage. Use an automatic control spray tower. Calibrate the spray volume before the experiment. Perform foliar spraying according to the experimental design, from low to high dose. Simultaneously, use a 0.1% Tween 80 aqueous solution containing an equal amount of organic solvent as a blank control. Each treatment is replicated 4 times.

[0016] Survey and statistics: The growth status of the tested weeds was observed and recorded regularly after treatment. Fourteen days after treatment, the above-ground weeds were harvested and their fresh weight was measured. The control efficacy was calculated based on the fresh weight.

[0017] Fresh weight control efficacy = (average fresh weight of weeds in the blank control area - average fresh weight of weeds in the treatment area) / average fresh weight of weeds in the blank control area × 100%.

[0018] Regression analysis was performed on the logarithmic value of the pesticide dosage (x) and the probability value of the weed control effect (y) to obtain the effective dosage (GR50) that inhibits weed fresh weight by 50%.

[0019] The type of synergistic effect of the compound is calculated using the co-toxicity coefficient method according to the following formula.

[0020] Toxicity index = GR50 of standard reagent / GR50 of test reagent × 100; Co-toxicity coefficient of the mixture = Measured value of toxicity index of the mixture / Theoretical value of toxicity index of the mixture × 100.

[0021] The theoretical value of the toxicity index of a mixture = toxicity index of A × proportion of A in the mixture + toxicity index of B × proportion of B in the mixture.

[0022] Judgment criteria: a co-toxicity coefficient of less than 80 indicates an antagonistic effect, between 80 and 120 indicates an additive effect, and greater than 120 indicates a synergistic effect.

[0023] Results and Analysis: As shown in Table 1, the GR50 values ​​of oxazolidinone, oxazolidinone, and isoxaflutole against barnyardgrass were 3.46 g / mu, 1.25 g / mu, and 8.02 g / mu, respectively.

[0024] When the mass ratios of oxadiazon, oxadiazon, and isoxaflutole were 13:1:6, 11:1:8, and 9:1:10, the co-toxicity coefficients against barnyardgrass were 132.7, 176.3, and 144.4, respectively, demonstrating a significant synergistic effect. The synergistic effect was particularly pronounced when the mass ratio of oxadiazon, oxadiazon, and isoxaflutole was 11:1:8. When the ratios of oxadiazon, oxadiazon, and isoxaflutole were other, the effect on barnyardgrass was additive.

[0025] Table 1. Indoor bioassays of the herbicidal composition against barnyardgrass.

[0026] Example 2: Indoor bioassay of herbicide composition on Echinochloa crus-galli and Cyperus difformis Test reagents: oxazolidinyl, oxazolidinyl, and isoxaflutole; all of the above components were purchased from the market.

[0027] Test targets: Echinochloa crus-galli and Cyperus difformis, collected from the field for later use.

[0028] Cultivation conditions: Select several plastic pots, add sterilized soil, and use bottom drip irrigation to fully moisten the soil. Sow weed seeds evenly on the soil surface, cover with an appropriate amount of sifted fine soil, and then move to a greenhouse for conventional cultivation. Water the pots using bottom drip irrigation. After a period of cultivation, thin the seedlings, leaving 25 seedlings per pot.

[0029] Chemical treatment: Foliar spraying was performed according to the designed dosage. An automatic control spray tower was used for spraying. The spray volume was calibrated before the experiment. Foliar spraying was performed according to the experimental design. At the same time, a blank control was prepared by treating with an aqueous solution of 0.1% Tween 80 containing an equal amount of organic solvent. Each treatment was replicated 4 times.

[0030] Survey and statistics: The growth status of the tested weeds was observed and recorded regularly after treatment. Fourteen days after treatment, the above-ground weeds were harvested and their fresh weight was measured. The control efficacy was calculated based on the fresh weight.

[0031] Fresh weight control efficacy = (average fresh weight of weeds in the blank control area - average fresh weight of weeds in the treatment area) / average fresh weight of weeds in the blank control area × 100%.

[0032] Data were statistically analyzed according to the "Guidelines for Indoor Bioassay Testing of Pesticides," and the Gowing method was used to test the combined effects of the herbicides and determine their synergistic efficacy. E0 represents the theoretical fresh weight control effect (hereinafter referred to as theoretical efficacy), and E represents the measured fresh weight control effect (hereinafter referred to as actual efficacy). Where E0 = a + ba × b / 100, a and b represent the measured fresh weight control effects of the two agents at different dosages, respectively; When -10 ≤ E - E0 ≤ 10, it is an additive effect; when E - E0 > 10, it is an synergistic effect; and when E - E0 < -10, it is an antagonistic effect. Results and Analysis: As shown in Table 2-3, oxadiazon, oxadiazon, and isoxaflutole, when used alone or in combination, have poor control effects on grasses and sedges. However, when oxadiazon, oxadiazon, and isoxaflutole technical grade herbicides are used in combination, the efficacy of the herbicidal composition is significantly improved, and a significant synergistic effect is observed. This can effectively reduce the dosage of active ingredients, reduce the development of herbicide resistance in weeds, and reduce environmental pollution.

[0033] Table 2. Indoor bioassays of the herbicidal composition on Echinochloa crus-galli.

[0034] Table 3. Indoor bioassays of the herbicidal composition on Cyperus difformis.

[0035] Example 3 Field efficacy test of the herbicidal composition against weeds in direct-seeded rice fields Experimental site: Direct-seeded rice field. Types of weeds in the field: Barnyard grass, Stellaria media, Echinochloa crus-galli, Cyperus difformis.

[0036] Test reagents 30% EC: Contains the following components by weight percentage: oxazolidinone 16.5%, oxazolidinone 1.5%, isoxaflutole 12%, agricultural emulsion 500# 6%, Tween 80 4%, dimethylformamide 2%, and methyl oleate to make up 100%.

[0037] Experimental methods: The plot area was 25m2, randomly arranged in a block design, with 3 replicates per treatment, and a water control was included. Manual foliar spraying was used. The fresh weight control efficacy of weeds was assessed 14 days after spraying. Five points were set in each plot, with each point covering 0.2m2, and the fresh weight of the aboveground parts of the weeds was measured to calculate the fresh weight control efficacy.

[0038] Fresh weight control efficacy = (fresh weight of weeds in control area - fresh weight of weeds in treatment area) / fresh weight of weeds in control area × 100%.

[0039] Results and Analysis: As shown in Table 5, the active ingredient of the 30% emulsifiable concentrate of the present invention has excellent control effect on rice weeds when the dosage is 10g / mu and 12g / mu, and the control efficacy is greater than 90%.

[0040] Table 4 Field control efficacy of herbicidal compositions against weeds in rice paddies

[0041] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it.

Claims

1. A herbicidal composition comprising oxazolidinone, oxazolidinone, and isoxaflutole, characterized in that, The active ingredients contain the following mass ratio: oxazolidinyl acetamiprid, oxazolidinyl acetamiprid and isoxaflutole in a mass ratio of 9-13:1:6-10.

2. The herbicidal composition according to claim 1, characterized in that, The mass ratio of oxazolidinyl acetamiprid, oxazolidinyl acetamiprid and isoxaflutole is 11:1:

8.

3. The herbicidal composition according to any one of claims 1-2, characterized in that, The herbicidal composition contains 1-75% of the active ingredient and includes adjuvants that are available in pesticides.

4. The herbicidal composition according to claim 3, characterized in that, The additive components include one or more of the following: dispersant, wetting agent, emulsifier, thickener, binder, disintegrant, preservative, solubilizer, antifreeze, filler, and solvent.

5. The herbicidal composition according to claim 4, characterized in that, The formulation of the herbicidal composition can be emulsifiable concentrate, suspension concentrate, dispersible oil suspension concentrate, wettable powder, or water-dispersible granule.

6. Use of the herbicidal composition according to any one of claims 1-5 for controlling weeds in paddy fields.

7. The use according to claim 6, characterized in that, The weeds mentioned are grass weeds, broadleaf weeds, and sedge weeds.

8. The use according to claim 7, characterized in that, The weeds mentioned are barnyard grass, Echinochloa crus-galli, Stellaria media, Cyperus difformis, Acer palmatum, and Monochoria vaginalis.

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