A nematicidal composition containing cyclobutrifluram and its application

Abstract

This invention belongs to the field of pesticide insecticide technology and discloses a nematicidal composition containing cyclobutrifluram and its application. The active ingredients of the composition include active ingredient A and active ingredient B. Active ingredient A is cyclobutrifluram, and active ingredient B is any one of the following compounds: (Formula 1), (Formula 2), (Formula 3), (Formula 4), and (Formula 5). The mass ratio of active ingredient A to active ingredient B is 1:50 to 50:1. The nematicidal composition of this invention has a significant synergistic effect on nematodes that harm plants, low toxicity, effectively slows down the development of pesticide resistance in target pests, and reduces the occurrence of soil-borne diseases.

Description

Technical Field

[0001] This invention belongs to the field of pesticide compound technology, specifically relating to a nematicide composition containing cyclobutrifluram and its application. Background Technology

[0002] Plant nematodes are tiny invertebrates that mostly live in soil or plant tissues, making them difficult to detect with the naked eye. Common species include root-knot nematodes and stem nematodes. They pierce plant cells with their stylets, sucking sap for nutrients, making them highly insidious pathogens in agricultural production. Their damage is extremely destructive, causing root malformation, yellowing and wilting of stems and leaves, and spreading fungi and bacteria, exacerbating disease outbreaks, and ultimately leading to reduced crop yields or even crop failure. They threaten a variety of crops, including vegetables, grains, and fruit trees.

[0003] Nematodes pose significant challenges to agricultural nematode control due to their wide host range, rapid reproduction, and tendency to accumulate in soil. Their tiny size and location within plants or deep in the soil make them difficult to reach with conventional pesticides. Furthermore, the long-term use of single-method control approaches can lead to pesticide resistance in nematodes, and some measures may disrupt soil ecosystems, creating a major challenge for integrated pest management. Summary of the Invention

[0004] To address the above problems, this invention provides a nematicidal composition containing cyclobutrifluram. This composition is a rational blend of compounds with different mechanisms of action, exhibiting a significant synergistic effect against nematodes that harm plants. It has low toxicity, effectively slows down the development of drug resistance in target pests, and reduces the occurrence of soil-borne diseases.

[0005] This invention also provides the application of the above-mentioned nematicidal composition for the control of root-knot nematodes, cyst nematodes, etc. on plants.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a nematicidal composition containing cyclobutrifluram, wherein the active ingredients of the composition include active ingredient A and active ingredient B, wherein active ingredient A is cyclobutrifluram, and active ingredient B is any one of the following compounds: (Formula 1) (Formula 2) (Formula 3) (Formula 4) (Formula 5); the mass ratio of active ingredient A to active ingredient B is 1:50 to 50:1.

[0007] Furthermore, the active ingredient B is a compound of formula 1, and the mass ratio of active ingredient A to active ingredient B is 1:40~30:1; The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:36~35:1; The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:30~50:1; The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:48~45:1; The active ingredient B is a compound of formula 5, and the mass ratio of active ingredient A to active ingredient B is 1:50~35:1.

[0008] Furthermore, the active ingredient B is a compound of formula 1, and the mass ratio of active ingredient A to active ingredient B is 1:30~20:1; The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:24~15:1; The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:25~32:1; The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:36~35:1; The active ingredient B is a compound of formula 5, and the mass ratio of the active ingredient A to the active ingredient B is 1:35~25:1; Preferably, the active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 10:1; The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:12 to 8:1. The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:25~12:1; The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:20~24:1; The active ingredient B is a compound of formula 5, and the mass ratio of active ingredient A to active ingredient B is 1:25 to 25:1.

[0009] Further, the active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:40, 1:30, 1:20, 1:10, 1:5, 1:2, 5:1, 10:1, 20:1, or 30:1. The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:36, 1:24, 1:12, 1:6, 2:5, 5:1, 8:1, 15:1, 24:1, or 35:1. The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:30, 1:25, 1:15, 1:8, 1:3, 6:1, 12:1, 25:1, 32:1, or 50:1. The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:48, 1:36, 1:20, 1:10, 1:4, 2:3, 6:1, 12:1, 24:1, 35:1, or 45:1. The active ingredient B is a compound of formula 5, and the mass ratio of active ingredient A to active ingredient B is 1:50, 1:35, 1:25, 1:18, 1:12, 2:15, 10:1, 15:1, 25:1, or 35:1.

[0010] Further, the active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:30, 1:20, 1:10, 1:5, 1:2, 5:1, 10:1, or 20:1; The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:24, 1:12, 1:6, 2:5, 5:1, 8:1, or 15:1. The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:25, 1:15, 1:8, 1:3, 6:1, 12:1, 25:1, or 32:1. The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:36, 1:20, 1:10, 1:4, 2:3, 6:1, 12:1, 24:1, or 35:1. The active ingredient B is a compound of formula 5, and the mass ratio of the active ingredient A to the active ingredient B is 1:35, 1:25, 1:18, 1:12, 2:15, 10:1, 15:1, or 25:1. Preferably, the active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:10, 1:5, 1:2, 5:1, or 10:1. The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:12, 1:6, 2:5, 5:1, or 8:1. The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:25, 1:15, 1:8, 1:3, 6:1, or 12:1. The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:20, 1:10, 1:4, 2:3, 6:1, 12:1, or 24:1. The active ingredient B is a compound of formula 5, and the mass ratio of active ingredient A to active ingredient B is 1:25, 1:18, 1:12, 2:15, 10:1, 15:1, or 25:1.

[0011] Furthermore, the total weight of active ingredient A and active ingredient B accounts for 0.1% to 80% of the composition; Furthermore, the total weight of active ingredient A and active ingredient B accounts for 0.1% to 60% of the composition.

[0012] Furthermore, in addition to the active ingredient, the composition also includes agriculturally permissible auxiliary ingredients selected from one or more of wetting agents, dispersants, thickeners, disintegrants, emulsifiers, defoamers, preservatives, stabilizers, synergists, and carriers.

[0013] Furthermore, the composition is prepared into a pesticide-permitted formulation, wherein the formulation is a solid or liquid formulation.

[0014] Furthermore, the solid formulation is a water-dispersible granule, wettable powder, or pellet; the liquid formulation is a suspension concentrate, emulsifiable concentrate, water emulsion, seed treatment suspension, microemulsion, or dispersible oil suspension.

[0015] The present invention also discloses the application of the nematicidal composition described above in the control of plant nematodes.

[0016] Furthermore, the nematode is any one or more of the following: root-knot nematode (Meloidogyne), cyst nematode (Heterodera), smooth blade nematode (Aphelenchina), stem nematode (Ditylenchus), root nodule nematode (Nacobbus), root rot nematode (Pratylenchus), and pine wood nematode (Bursa phelenchus xylophilus); Furthermore, the nematode mentioned is a root-knot nematode.

[0017] The beneficial effects of this invention are as follows: The nematicidal composition of the present invention can synergistically enhance nematicidal activity, significantly improve the control effect on root-knot nematodes and other nematodes, effectively reduce the nematode population; reduce the dosage of single components, reduce pesticide residues and environmental pressure; delay the development of nematode resistance, extend the life of the agent; at the same time, protect the crop root system, reduce the damage caused by superimposed diseases, help increase crop yield and quality, and have lower application costs and greater practicality. Detailed Implementation

[0018] To better illustrate the effective control effect of the present invention, the agents described in the above embodiments are used. To make the technical solution, objectives, and advantages of the present invention clearer, the present invention is illustrated with the following specific embodiments, but the present invention is not limited to these examples. The efficacy experiments of the present invention employ a combination of indoor bioassays and field trials.

[0019] The following is a preparation process for a formulation.

[0020] 1. Suspension formulation processing technology: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling, and finally homogenized filtration to obtain the required suspension.

[0021] 2. Processing technology of emulsifiable concentrate formulation: According to the formula ratio, the measured active ingredients, solvents and cosolvents are added to the mixing tank and stirred to dissolve them. Then the emulsifier is added, and the remaining solvent is used to make up the balance. The mixture is stirred evenly in the mixing tank and filtered to obtain the desired emulsifiable concentrate.

[0022] 3. Granule formulation processing technology: According to the formula ratio, the active ingredients, dispersants and additives are mixed evenly to form a master powder. The master powder and carrier are added together to the coating granulator and stirred. While stirring, the prepared binder aqueous solution is added. After being fully mixed, the material is discharged, dried and sieved to obtain the granules of the present invention.

[0023] 4. Water-dispersible granule processing technology: According to the formula ratio, the active ingredients are added to the carrier, and surfactants and other functional additives are added to it. After mixing, the mixture is pulverized by air jet milling, and 10-25% water is added. Then, the mixture is kneaded, granulated, dried and sieved to obtain water-dispersible granule products; or the pulverized powder is sprayed with water, granulated and dried in a fluidized bed granulator, and then sieved to obtain water-dispersible granule products.

[0024] 5. Water-in-oil emulsion processing technology: According to the formula ratio, dissolve the active ingredients in the solvent and add the emulsifier to dissolve them into a uniform oil phase. Mix deionized water, antifreeze, etc. together to form a uniform aqueous phase. Under high-speed shearing, add the oil phase to the aqueous phase and shear until the particle size is qualified. Then add the defoamer, thickener, and preservative and stir evenly to form a well-dispersed water-in-oil emulsion product.

[0025] 6. Microemulsion processing technology: According to the formula ratio, the active ingredients, solvents, emulsifiers, etc. are mixed evenly to obtain the oil phase. The antifreeze and water are mixed evenly to obtain the water phase. The oil phase is added to the water phase under stirring and stirred evenly. Shearing is continued for 10 minutes. Then, the defoamer is added and stirred evenly to obtain small droplets with oil phase particles of 0.01~0.1 micrometers, which is the microemulsion product.

[0026] Example of formulation preparation.

[0027] Preparation Example 1: 12% cyclobutrifluram·Formula 1 compound suspension (1:2) Formula composition: 4% cyclobutrifluram, 8% compound of formula 1, 2% sodium dodecyl sulfate, 1% sodium alkyl polyoxyethylene ether sulfonate, 4% arylphenol polyoxyethylene ether phosphate, 0.25% xanthan gum, 1% magnesium aluminum silicate, 5% ethylene glycol, 0.01% potassium benzisothiazolinone, 0.5% silicone oil, deionized water to make up the balance.

[0028] Preparation Example 2: 11% cyclobutrifluram·formula 1 compound emulsion (1:10) Formulation composition: 1% cyclobutrifluram, 10% compound of formula 1, 15% DMF, 15% sorbitan oleate polyoxyethylene ether, 2% naphthalene sulfonate formaldehyde condensate, 20% propylene carbonate, xylene to make up the balance.

[0029] Preparation Example 3: 18% Cyclobutrifluram Formula 1 Compound Granules (5:1) Formula composition: 15% cyclobutrifluram, 3% compound of formula 1, 4% sodium lignosulfonate, 3% potassium humate, 15% silica, 3% carboxymethyl cellulose, and diatomaceous earth granules to make up the balance.

[0030] Preparation Example 4: 16% cyclobutrifluram·Formula 3 compound suspension (1:15) Formula composition: 1% cyclobutrifluram, 15% Formula 3 compound, 2% tristyrylphenol polyoxyethylene ether, 3% arylphenol polyoxyethylene ether phosphate, 1% lignin sulfonate, 2% glycerol fatty acid ester polyoxyethylene ether, 0.25% xanthan gum, 5% glycerol, 0.25% sodium benzoate, 0.5% silicone oil, deionized water to make up the balance.

[0031] Preparation Example 5: 12% cyclobutrifluram·formula 3 compound microemulsion (1:3) Formula composition: 3% cyclobutrifluram, 9% Formula 3 compound, 20% cyclohexanone, 8% phenylethylphenol polyoxyethylene polyoxypropylene ether, 3% Gelbert alcohol polyoxyethylene ether, 1% alkylphenol formaldehyde resin polyoxyethylene ether sulfate, 0.2% silicone defoamer, deionized water to make up the balance.

[0032] Preparation Example 6: 13% Cyclobutrifluram Formula 3 Compound Granules (12:1) Formula composition: 12% cyclobutrifluram, 1% Formula 3 compound, 5% succinate sulfonate, 5% sodium alginate, and attapulgite particles to make up the balance.

[0033] Preparation Example 7: 22% cyclobutrifluram·Formula 4 compound suspension (1:10) Formula composition: 2% cyclobutrifluram, 20% Formula 4 compound, 2% dehydrated sorbitan oleate polyoxyethylene ether, 3% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 3% arylphenol polyoxyethylene ether phosphate, 1% sodium polycarboxylate, 2% magnesium aluminum silicate, 0.15% carboxyethyl cellulose, 1% sodium sorbate, 5% glycerol, 0.5% silicone oil, deionized water to make up the balance.

[0034] Preparation Example 8: 15% cyclobutrifluram·Formula 4 compound water-dispersible granules (2:3) Formula composition: 6% cyclobutrifluram, 9% Formula 4 compound, 10% sodium lignosulfonate, 2% BX (a type of lignosulfonate), 1% fatty alcohol polyoxyethylene ether sulfate, 5% ammonium sulfate, and kaolin to make up the balance.

[0035] Preparation Example 9: 19.5% cyclobutrifluram·formula 4 compound granules (12:1) Formula composition: 18% cyclobutrifluram, 1.5% Formula 4 compound, 5% succinate sulfonate, 3% polyvinyl alcohol, 3% sodium lignosulfonate, 10% silica, and ceramic sand particles to make up the balance.

[0036] Preparation Example 10: 13% cyclobutrifluram·Formula 2 compound suspension (1:12) Formula composition: 1% cyclobutrifluram, 12% Formula 2 compound, 2% ethylene glycol oxyethylene polyoxypropylene ether, 1% sodium octylphenol polyoxyethylene ether sulfonate, 3% glycerol fatty acid ester polyoxyethylene ether phosphate, 2% sorbitan oleate polyoxyethylene ether, 0.25% xanthan gum, 1% magnesium aluminum silicate, 5% ethylene glycol, 0.3% potassium benzisothiazolinone, 0.5% silicone oil, deionized water to make up the balance.

[0037] Preparation Example 11: 7% cyclobutrifluram·Formula 2 compound aqueous emulsion (2:5) Formula composition: 2% cyclobutrifluram, 5% Formula 2 compound, 5% glycerol fatty acid ester polyoxyethylene ether, 3% arylphenol polyoxyethylene ether phosphate, 12% cyclohexanone, 0.25% xanthan gum, 0.1% isothiazolinone, 5% ethylene glycol, 1.5% urea, 0.5% sodium sorbate, 0.1% silicone defoamer, deionized water to make up the balance.

[0038] Preparation Example 12: 18% Cyclobutrifluram Formula 2 Compound Granules (8:1) Formula composition: 16% cyclobutrifluram, 2% compound of formula 2, 5% naphthalene sulfonate formaldehyde condensate NNO, 2% polyvinylpyrrolidone, bentonite granules to make up the balance.

[0039] Preparation Example 13: 17% cyclobutrifluram·Formula 5 compound suspension (2:15) Formula composition: 2% cyclobutrifluram, 15% Formula 5 compound, 2% isomeric tridecyl alcohol polyoxyethylene ether, 2% glycerol fatty acid ester polyoxyethylene ether, 1% fatty alcohol polyoxyethylene ether sulfate, 5% castor oil polyoxyethylene ether phosphate, 1.5% magnesium aluminum silicate, 0.2% carboxyethyl cellulose, 1% sodium sorbate, 5% ethylene glycol, 0.5% silicone oil, deionized water to make up the balance.

[0040] Preparation Example 14: 19% cyclobutrifluram·Formula 5 compound microemulsion (1:18) Formula composition: 1% cyclobutrifluram, 18% Formula 5 compound, 12% xylene, 20% cyclohexanone, 15% glycerol fatty acid ester polyoxyethylene ether, 5% EO-PO block copolymer, 1% sodium alkyl polyoxyethylene ether sulfonate, 5% ethylene glycol, 0.05% silicone defoamer, deionized water to make up the balance.

[0041] Preparation Example 15: 22% Cyclobutrifluram Formula 5 Compound Granules (10:1) Formula composition: 20% cyclobutrifluram, 2% Formula 5 compound, 5% naphthalene sulfonate formaldehyde condensate, 2% polyvinyl acetate, 9% silica, and diatomaceous earth particles to make up the balance.

[0042] Example 1: Combined toxicity test on southern root-knot nematodes.

[0043] Experimental basis: The experiment was conducted in accordance with NY / T 1833.1-2009 "Standard for Indoor Bioassay Tests of Pesticides - Nematicides Part 1: Inhibition of Plant Pathogenic Nematodes - Immersion Method".

[0044] Test subject: Southern root-knot nematode [ Meloidogyne incongnita (Kofold&White)Chitwood].

[0045] Test reagents: cyclobutrifluram, compound of formula 1, compound of formula 2, compound of formula 3, compound of formula 4, and compound of formula 5 (original drug).

[0046] Reagent preparation: Dissolve each of the above reagents in a suitable solvent first, then dilute with a 0.1% Tween-80 aqueous solution. Set up 5 series concentration gradients for each reagent, with an aqueous solution containing no reagent and the same solvent content as a control.

[0047] Preparation of nematode suspension: Root-knot nematode egg masses were picked from cucumber roots severely affected by root-knot nematodes, washed with clean water, placed on filter paper in a petri dish, and an appropriate amount of distilled water was added. The dish was then incubated in an incubator at 25±1℃. After 24 hours, second-instar larvae of the same age were collected to prepare a root-knot nematode suspension. Each 1 mL of suspension contained no less than 100 nematodes. The suspension was then set aside for later use.

[0048] Experimental Method: Using a pipette, 3 mL of the drug solution was added sequentially from low to high concentration to each test tube. Then, 3 mL of the prepared nematode suspension was added to each test tube, ensuring equal volumes of drug and nematode suspension were thoroughly mixed. A specific volume of the mixture was then pipetted into the wells of a multi-well biochemical test plate, which was then capped. Each treatment was repeated four times, with an aqueous solution containing the same solvent and no drug as a control. The plates were incubated at 25°C for 24 hours, and the activity of the nematodes was examined. The relative lethality of each drug treatment was calculated.

[0049] Experimental investigation: Take 1 mL of the mixture from each treatment and observe the mortality of nematodes under a dissecting microscope. The number of nematodes observed in each replicate should not be less than 100. Record the total number of nematodes observed and the number of dead nematodes. Dead nematodes are often stiff and cannot bend or move when touched with a hair-thread needle or bamboo needle.

[0050] Calculation method: Based on the survey data, the mortality rates for each treatment were calculated using the following formula, with all results rounded to two decimal places:

[0051] If the control mortality rate is less than 5%, no correction is needed; if the control mortality rate is between 5% and 10%, correction should be performed according to the corrected mortality rate formula; if the control mortality rate is greater than 15%, the trial needs to be repeated.

[0052] Statistical analysis: Based on the logarithmic values ​​of each drug concentration and the corresponding mortality rate, a statistical analysis system was used to determine the toxicity regression line and LC. 50The values ​​and correlation coefficients are used to evaluate the activity of the test reagent on the biological sample.

[0053] The co-toxicity coefficient (CTC) of the mixture was calculated using Sun Yunpei's method to evaluate the type of combined effects: A CTC ≥ 120 indicates a synergistic effect, a CTC ≤ 80 indicates an antagonistic effect, and a CTC between 80 and 120 indicates an additive effect.

[0054] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0055] In the formula: ATI —Measured toxicity index of the mixture; S LC of standard insecticides 50 The unit is milligrams per liter (mg / L); M LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0056]

[0057] In the formula: TTI —Theoretical toxicity index of the mixture; TI A —A. Toxicity index of drug A; P A —The percentage content of drug A in the mixture, expressed as a percentage (%). TI B —Toxicity index of drug B; P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0058]

[0059] In the formula: CTC —Cotoxicity coefficient; ATI —Measured toxicity index of the mixture; TTI —Theoretical toxicity index of mixed preparations.

[0060] The test results are shown in the table below.

[0061] Table 1. Indoor bioactivity assays of cyclobutrifluram and compound of formula 1 against southern root-knot nematodes. cyclobutrifluram (A) / / y = 4.6924 + 1.5389x 0.9933 1.5843 / Compound of Formula 1 (B) / / y = 4.5315 + 1.6151x 0.9986 1.9503 / A:B 1 40 y = 4.7074 + 1.4147x 0.9907 1.6100 120.458 A:B 1 30 y = 4.7877 + 1.3962x 0.9885 1.4192 136.406 A:B 1 20 y = 4.8097 + 1.5039x 0.9902 1.3382 144.155 A:B 1 10 y = 4.8650 + 1.5097x 0.9897 1.2287 155.464 A:B 1 5 y = 5.0158 + 1.4861x 0.9999 0.9759 192.437 A:B 1 2 y = 5.0806 + 1.5760x 0.9979 0.8889 203.719 A:B 5 1 y = 5.0598 + 1.4606x 0.9962 0.9100 179.720 A:B 10 1 y = 4.9860 + 1.5035x 0.9987 1.0217 157.756 A:B 20 1 y = 4.8954 + 1.4179x 0.9930 1.1852 134.879 A:B 30 1 y = 4.8276 + 1.4773x 0.9886 1.3082 121.843 A:B 50 1 y = 4.7559 + 1.4996x 0.9908 1.4547 109.311 Indoor experiments showed that the combination of cyclobutrifluram and compound of formula 1 exhibited good activity against southern root-knot nematodes. When the mass ratio of cyclobutrifluram to compound of formula 1 was 1:40–30:1, the co-toxicity coefficient was greater than 120, indicating a synergistic effect; when the mass ratio was 1:30–20:1, the co-toxicity coefficient was greater than 130, showing a significant synergistic effect; and when the mass ratio was 1:10–10:1, the co-toxicity coefficient was greater than 150, indicating a remarkable synergistic effect.

[0062] Among them, the mass ratios of cyclobutrifluram to the compound of formula 1 are 1:40, 1:30, 1:20, 1:10, 1:5, 1:2, 5:1, 10:1, 20:1, and 30:1, with a co-toxicity coefficient greater than 120, showing a synergistic effect; the mass ratios of cyclobutrifluram to the compound of formula 1 are 1:30, 1:20, 1:10, 1:5, 1:2, 5:1, 10:1, and 20:1, with a co-toxicity coefficient greater than 130, showing a significant synergistic effect; the mass ratios of cyclobutrifluram to the compound of formula 1 are 1:10, 1:5, 1:2, 5:1, and 10:1, with a co-toxicity coefficient greater than 150, showing a significant synergistic effect.

[0063] Table 2. Indoor bioactivity assays of cyclobutrifluram and compound of formula 3 against southern root-knot nematodes. cyclobutrifluram (A) / / y = 4.6924 + 1.5389x 0.9933 1.5843 / Compound of Formula 3 (B) / / y = 4.6739 + 1.2471x 0.9948 1.8260 / A:B 1 50 y = 4.5784 + 1.5708x 0.9973 1.8551 98.138 A:B 1 30 y = 4.7947 + 1.3876x 0.9941 1.4059 129.245 A:B 1 25 y = 4.8817 + 1.3096x 0.9951 1.2311 147.457 A:B 1 15 y = 4.9518 + 1.4436x 0.9995 1.0799 167.493 A:B 1 8 y = 5.0312 + 1.3724x 0.9972 0.9490 189.206 A:B 1 3 y = 5.0897 + 1.4019x 0.9967 0.8630 203.814 A:B 6 1 y = 5.1091 + 1.4580x 0.9983 0.8417 191.854 A:B 12 1 y = 5.0255 + 1.4171x 0.9952 0.9594 166.833 A:B 25 1 y = 4.9081 + 1.5473x 0.9957 1.1466 138.881 A:B 32 1 y = 4.8569 + 1.6751x 0.9903 1.2173 130.673 A:B 50 1 y = 4.8476 + 1.5014x 0.9941 1.2633 125.736 Indoor experiments showed that the combination of cyclobutrifluram and compound 3 exhibited good activity against southern root-knot nematodes. When the mass ratio of cyclobutrifluram to compound 3 was 1:30–50:1, the co-toxicity coefficient was greater than 120, indicating a synergistic effect; when the mass ratio was 1:25–32:1, the co-toxicity coefficient was greater than 130, showing a significant synergistic effect; and when the mass ratio was 1:25–12:1, the co-toxicity coefficient was greater than 140, indicating a remarkable synergistic effect.

[0064] Among them, the mass ratios of cyclobutrifluram to the compound of formula 3 are 1:30, 1:25, 1:15, 1:8, 1:3, 6:1, 12:1, 25:1, 32:1, and 50:1, with a co-toxicity coefficient greater than 120, showing a synergistic effect; the mass ratios of cyclobutrifluram to the compound of formula 3 are 1:25, 1:15, 1:8, 1:3, 6:1, 12:1, 25:1, and 32:1, with a co-toxicity coefficient greater than 130, showing a significant synergistic effect; the mass ratios of cyclobutrifluram to the compound of formula 3 are 1:25, 1:15, 1:8, 1:3, 6:1, and 12:1, with a co-toxicity coefficient greater than 140, showing a significant synergistic effect.

[0065] Table 3. Indoor bioactivity assays of cyclobutrifluram and compound of formula 4 against southern root-knot nematodes. cyclobutrifluram (A) / / y = 4.6924 + 1.5389x 0.9933 1.5843 / Compound of Formula 4 (B) / / y = 4.5314 + 1.5106x 0.9994 2.0426 / A:B 1 48 y = 4.6686 + 1.5726x 0.9965 1.6246 124.992 A:B 1 36 y = 4.7119 + 1.6200x 0.9922 1.5061 134.570 A:B 1 20 y = 4.8465 + 1.3844x 0.9962 1.2909 156.081 A:B 1 10 y = 4.8864 + 1.3908x 0.9967 1.2070 164.893 A:B 1 4 y = 4.9623 + 1.6359x 0.9971 1.0545 183.109 A:B 2 3 y = 5.0366 + 1.4548x 0.9997 0.9437 193.998 A:B 6 1 y = 4.9915 + 1.4390x 0.9832 1.0137 161.464 A:B 12 1 y = 4.9564 + 1.5896x 0.9970 1.0652 151.345 A:B 24 1 y = 4.9444 + 1.4082x 0.9912 1.0952 145.969 A:B 35 1 y = 4.8874 + 1.6306x 0.9805 1.1724 135.981 A:B 45 1 y = 4.8334 + 1.6498x 0.9801 1.2617 126.184 Indoor experiments showed that the combination of cyclobutrifluram and compound 4 exhibited good activity against southern root-knot nematodes. When the mass ratio of cyclobutrifluram to compound 4 was 1:48–45:1, the co-toxicity coefficient was greater than 120, indicating a synergistic effect; when the mass ratio was 1:36–35:1, the co-toxicity coefficient was greater than 130, showing a significant synergistic effect; and when the mass ratio was 1:20–24:1, the co-toxicity coefficient was greater than 140, also showing a remarkable synergistic effect.

[0066] Among them, the mass ratios of cyclobutrifluram to compounds of formula 4 are 1:48, 1:36, 1:20, 1:10, 1:4, 2:3, 6:1, 12:1, 24:1, 35:1, and 45:1, with a co-toxicity coefficient greater than 120, exhibiting a synergistic effect; the mass ratios of cyclobutrifluram to compounds of formula 4 are 1:36, 1:20, 1:10, 1:4, 2:3, 6:1, 12:1, 24:1, and 35:1, with a co-toxicity coefficient greater than 130, showing a significant synergistic effect; the mass ratios of cyclobutrifluram to compounds of formula 4 are 1:20, 1:10, 1:4, 2:3, 6:1, 12:1, and 24:1, with a co-toxicity coefficient greater than 140, showing a significant synergistic effect.

[0067] Table 4. Indoor bioactivity assays of cyclobutrifluram and compound of formula 2 against southern root-knot nematodes. cyclobutrifluram (A) / / y = 4.6924 + 1.5389x 0.9933 1.5843 / Compound of Formula 2 (B) / / y = 4.46 0.9885 2.2814 / ​ 1 48 ​ 0.9965 2.6273 86.062 ​ 1 36 ​ 0.9983 1.7843 126.357 ​ 1 24 ​ 0.9966 1.6494 135.925 ​ 1 12 ​ 0.9948 1.3917 158.562 ​ 1 6 ​ 0.9930 1.3149 163.243 A:B 2 5 y = 4.9408 + 1.5427x 0.9985 1.0924 185.520 A:B 5 1 y = 4.9430 + 1.6476x 0.9852 1.0829 154.152 A:B 8 1 y = 4.9101 + 1.6238x 0.9812 1.1359 144.377 A:B 15 1 y = 4.8984 + 1.5492x 0.9996 1.1636 138.806 A:B 24 1 y = 4.8552 + 1.5453x 0.9989 1.2408 129.264 A:B 35 1 y = 4.8265 + 1.4979x 0.9982 1.3056 122.385 Indoor experiments showed that the combination of cyclobutrifluram and compound 2 exhibited good activity against southern root-knot nematodes. When the mass ratio of cyclobutrifluram to compound 2 was 1:36–35:1, the co-toxicity coefficient was greater than 120, indicating a synergistic effect; when the mass ratio was 1:24–15:1, the co-toxicity coefficient was greater than 130, showing a significant synergistic effect; and when the mass ratio was 1:12–8:1, the co-toxicity coefficient was greater than 140, indicating a remarkable synergistic effect.

[0068] Among them, the mass ratios of cyclobutrifluram to the compound of formula 2 are 1:36, 1:24, 1:12, 1:6, 2:5, 5:1, 8:1, 15:1, 24:1, and 35:1, with a co-toxicity coefficient greater than 120, showing a synergistic effect; the mass ratios of cyclobutrifluram to the compound of formula 2 are 1:24, 1:12, 1:6, 2:5, 5:1, 8:1, and 15:1, with a co-toxicity coefficient greater than 130, showing a significant synergistic effect; the mass ratios of cyclobutrifluram to the compound of formula 2 are 1:12, 1:6, 2:5, 5:1, and 8:1, with a co-toxicity coefficient greater than 140, showing a significant synergistic effect.

[0069] Table 5. Indoor bioactivity assays of cyclobutrifluram and compound of formula 5 against southern root-knot nematodes. cyclobutrifluram (A) / / y = 4.6924 + 1.5389x 0.9933 1.5843 / Compound (B) of Formula 5 / / y = 4.3881 + 1.4964x 0.9991 2.5640 / A:B 1 50 y = 4.5310 + 1.4831x 0.9931 2.0713 122.304 A:B 1 35 y = 4.4696 + 1.8542x 0.9955 1.9322 130.458 A:B 1 25 y = 4.5899 + 1.6665x 0.9965 1.7622 142.120 A:B 1 18 y = 4.6466 + 1.7069x 0.9996 1.6107 154.168 A:B 1 12 y = 4.7790 + 1.5797x 0.9968 1.3801 177.348 A:B 2 15 y = 4.8539 + 1.4554x 0.9895 1.2601 189.677 A:B 10 1 y = 5.0458 + 1.3997x 0.9976 0.9274 176.980 A:B 15 1 y = 4.9617 + 1.4234x 0.9979 1.0640 152.543 A:B 25 1 y = 4.9341 + 1.4936x 0.9952 1.1069 145.264 A:B 35 1 y = 4.8362 + 1.4350x 0.9904 1.3006 123.120 A:B 45 1 y = 4.7375 + 1.7420x 0.9983 1.4148 112.918 Indoor experiments showed that the combination of cyclobutrifluram and compound 5 exhibited good activity against southern root-knot nematodes. When the mass ratio of cyclobutrifluram to compound 5 was 1:50–35:1, the co-toxicity coefficient was greater than 120, indicating a synergistic effect; when the mass ratio was 1:35–25:1, the co-toxicity coefficient was greater than 130, showing a significant synergistic effect; and when the mass ratio was 1:25–25:1, the co-toxicity coefficient was greater than 140, also showing a remarkable synergistic effect.

[0070] Among them, the mass ratios of cyclobutrifluram to compounds of formula 5 of 1:50, 1:35, 1:25, 1:18, 1:12, 2:15, 10:1, 15:1, 25:1, and 35:1 have a co-toxicity coefficient greater than 120, exhibiting a synergistic effect; the mass ratios of cyclobutrifluram to compounds of formula 5 of 1:35, 1:25, 1:18, 1:12, 2:15, 10:1, 15:1, and 25:1 have a co-toxicity coefficient greater than 130, showing a significant synergistic effect; the mass ratios of cyclobutrifluram to compounds of formula 5 of 1:25, 1:18, 1:12, 2:15, 10:1, 15:1, and 25:1 have a co-toxicity coefficient greater than 140, showing a significant synergistic effect.

[0071] Example 2: Field efficacy test of pesticide for controlling root-knot nematodes in melons.

[0072] Experimental basis: The experiment was conducted in accordance with GB / T 17980.38-2000 "Field Efficacy Test Guidelines for Pesticides (I) Nematicides for the Control of Root Nematode Diseases".

[0073] Experimental target: root-knot nematodes ( Meloidogyne spp.).

[0074] Experimental crop: Melon (Elizabeth).

[0075] Experimental location: a solar greenhouse in Tianma Town, Shouguang City, Shandong Province. Greenhouses with a history of severe root-knot nematode disease and relatively even nematode distribution were selected as experimental greenhouses.

[0076] Experimental treatment: The experimental cells were arranged in a randomized block design, with a cell area of ​​30m². 2 Each treatment was repeated four times. Root irrigation was performed on the day the melons were transplanted, and the treatment was applied once throughout the experiment.

[0077] Investigation method: 60 days after application of the pesticide, suspected diseased plants were searched in the community. A random 5-point sampling method was used, with 2 plants investigated at each point. The incidence rate, disease index and control effect were calculated.

[0078] Methods for grading diseased plants: Grade 0: No insect galls on the roots; Grade 1: The root system has a small number of small insect galls; Level 3: Two-thirds of the root system is covered with small galls; Level 5: The root system is covered with small galls and secondary galls; Level 7: The root system forms a cluster of fibrous roots.

[0079] Methods for calculating drug efficacy:

[0080] The results of the field efficacy trials are shown in the table below: Table 6 Results of field efficacy trials against root-knot nematodes in melon Preparation Example 5: 12% cyclobutrifluram (Formula 3) microemulsion (1:3) 5 2.50 95.98 Preparation Example 1: 12% cyclobutrifluram (Formula 1) suspension (1:2) 5 3.57 94.25 Preparation Example 8: 15% cyclobutrifluram (Formula 4) water-dispersible granules (2:3) 5 5.36 91.38 Preparation Example 13: Cyclobutrifluram • Formula 5 compound suspension (2:15) 5 6.07 90.23 Preparation Example 11: 7% cyclobutrifluram • Formula 2 compound aqueous emulsion (2:5) 5 8.93 85.63 450g / L Cyclobutrifluram Suspension 7 12.50 79.89 20% Formula 1 Compound Suspension 8 15.71 74.71 18% Formula 3 Compound Suspension 8 17.50 71.84 12% Formula 4 compound water emulsion 8 20.00 67.82 20% Formula 2 compound water-dispersible granules 8 21.79 64.94 10% Formula 5 compound microemulsion 8 21.07 66.09 Blank control / 62.14 / The results of field efficacy trials show that the compound combination has a good control effect on melon root-knot nematodes, and the overall control efficacy of each compound agent treatment group against melon root-knot nematodes is over 85%.

[0081] Indoor bioassays and field efficacy tests show that the nematicidal composition of the present invention has a significant synergistic effect on plant nematode diseases, reducing the amount of single-agent application while reducing pesticide residues in agricultural products, and is safe and efficient.

[0082] The above description, in conjunction with specific preferred embodiments, provides a further detailed explanation of the present invention. It should not be construed that the specific implementation of the present invention is limited to these descriptions. For those skilled in the art, various simple deductions or substitutions can be made without departing from the concept of the present invention, and all such modifications and substitutions should be considered within the scope of protection of the present invention.

Claims

1. A nematicidal composition containing cyclobutrifluram, characterized in that, The active ingredients of the composition described herein include active ingredient A and active ingredient B, wherein active ingredient A is cyclobutrifluram and active ingredient B is any one of the following compounds: (Formula 1) (Formula 2) (Formula 3) (Formula 4) (Formula 5); the mass ratio of active ingredient A to active ingredient B is 1:50 to 50:

1.

2. The nematicide composition according to claim 1, characterized in that, The active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:40~30:1; The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:36~35:1; The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:30~50:1; The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:48~45:1; The active ingredient B is a compound of formula 5, and the mass ratio of active ingredient A to active ingredient B is 1:50~35:

1.

3. The nematicide composition according to claim 2, characterized in that, The active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:30~20:1; The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:24~15:1; The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:25~32:1; The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:36~35:1; The active ingredient B is a compound of formula 5, and the mass ratio of the active ingredient A to the active ingredient B is 1:35~25:1; Preferably, the active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 10:1; The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:12 to 8:

1. The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:25~12:1; The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:20~24:1; The active ingredient B is a compound of formula 5, and the mass ratio of active ingredient A to active ingredient B is 1:25 to 25:

1.

4. The nematicide composition according to claim 1, characterized in that, The active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:40, 1:30, 1:20, 1:10, 1:5, 1:2, 5:1, 10:1, 20:1, or 30:

1. The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:36, 1:24, 1:12, 1:6, 2:5, 5:1, 8:1, 15:1, 24:1, or 35:

1. The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:30, 1:25, 1:15, 1:8, 1:3, 6:1, 12:1, 25:1, 32:1, or 50:

1. The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:48, 1:36, 1:20, 1:10, 1:4, 2:3, 6:1, 12:1, 24:1, 35:1, or 45:

1. The active ingredient B is a compound of formula 5, and the mass ratio of active ingredient A to active ingredient B is 1:50, 1:35, 1:25, 1:18, 1:12, 2:15, 10:1, 15:1, 25:1, or 35:

1.

5. The nematicide composition according to claim 4, characterized in that, The active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:30, 1:20, 1:10, 1:5, 1:2, 5:1, 10:1, or 20:

1. The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:24, 1:12, 1:6, 2:5, 5:1, 8:1, or 15:

1. The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:25, 1:15, 1:8, 1:3, 6:1, 12:1, 25:1, or 32:

1. The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:36, 1:20, 1:10, 1:4, 2:3, 6:1, 12:1, 24:1, or 35:

1. The active ingredient B is a compound of formula 5, and the mass ratio of the active ingredient A to the active ingredient B is 1:35, 1:25, 1:18, 1:12, 2:15, 10:1, 15:1, or 25:

1. Preferably, the active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:10, 1:5, 1:2, 5:1, or 10:

1. The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:12, 1:6, 2:5, 5:1, or 8:

1. The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:25, 1:15, 1:8, 1:3, 6:1, or 12:

1. The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:20, 1:10, 1:4, 2:3, 6:1, 12:1, or 24:

1. The active ingredient B is a compound of formula 5, and the mass ratio of active ingredient A to active ingredient B is 1:25, 1:18, 1:12, 2:15, 10:1, 15:1, or 25:

1.

6. The nematicide composition according to claim 1, characterized in that, The total weight of active ingredient A and active ingredient B accounts for 0.1% to 80% of the composition, preferably 0.1% to 60%.

7. The nematicide composition according to claim 1, characterized in that, In addition to the active ingredient, the composition also includes agriculturally permissible auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, thickeners, disintegrants, emulsifiers, defoamers, preservatives, stabilizers, synergists, and carriers.

8. The nematicide composition according to claim 1, characterized in that, The composition is prepared into a pesticide formulation, which may be a solid or liquid formulation.

9. The nematicide composition according to claim 8, characterized in that, The solid formulation is a water-dispersible granule, wettable powder, or pellet; the liquid formulation is a suspension concentrate, emulsifiable concentrate, water emulsion, seed treatment suspension, microemulsion, or dispersible oil suspension.

10. The application of the nematicidal composition according to any one of claims 1-9 in the control of plant nematodes, characterized in that, The nematodes mentioned are any one or more of the following: root-knot nematode (Meloidogyne), cyst nematode (Heterodera), smooth blade nematode (Aphelenchina), stem nematode (Ditylenchus), root nodule nematode (Nacobbus), root rot nematode (Pratylenchus), and pine wood nematode (Bursa phelenchus xylophilus).

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