An environmentally friendly algae remover

Abstract

This invention belongs to the field of algaecide technology, specifically relating to an environmentally friendly algaecide. The active ingredient of this environmentally friendly algaecide is composed of dialcyl dimethyl ammonium chloride or polyhexamethylene biguanide hydrochloride and menthol in a mass ratio of 1-100:100-1. This invention scientifically combines dialcyl dimethyl ammonium chloride or polyhexamethylene biguanide hydrochloride with the natural terpene compound menthol. Menthol, as a natural plant-derived synergist, possesses certain algae-inhibiting activity and simultaneously enhances the activity of dialcyl dimethyl ammonium chloride or polyhexamethylene biguanide hydrochloride against algae. This helps to reduce the dosage while ensuring algae-removing effectiveness, thereby effectively mitigating the risks associated with using quaternary ammonium compounds for algae removal.

Description

Technical Field

[0001] This invention belongs to the field of algae removal technology, specifically relating to an environmentally friendly algae remover. Background Technology

[0002] Eutrophication can cause massive algal blooms and water quality deterioration. In artificial water environments such as swimming pools, landscape water bodies, and aquaculture water bodies, excessive algal growth not only affects water transparency and sensory experience, but also consumes dissolved oxygen and releases algal toxins, posing a threat to the ecological environment and human health.

[0003] Current technologies for controlling algal pollution mainly fall into three categories: physical, biological, and chemical methods. Among these, chemical methods have become an important approach for water environment management and the remediation of polluted water bodies due to their high algae removal efficiency and ease of use. Of the reported chemical algaecides, quaternary ammonium cationic surfactants, oxidizing bactericides (such as chlorine dioxide, hydrogen peroxide, and hypochlorite), heavy metal salts (such as copper sulfate), and some organic algaecides (such as diuron) have been widely studied and applied due to their broad-spectrum and highly effective algae-killing properties. Furthermore, with the development of environmentally friendly water treatment technologies, natural terpenoid allelochemicals derived from plant secondary metabolites have gradually become a research hotspot in the field of algaecide development due to their relative safety and ease of degradation.

[0004] While quaternary ammonium salts and heavy metal salts are effective algaecides, long-term and excessive use can lead to residues and accumulation in water bodies, posing potential risks to aquatic organisms and human health, which contradicts the development trend of environmentally friendly water treatment agents. Oxidative algaecides (such as chlorine dioxide and hydrogen peroxide) leave no harmful residues, but they are unstable, easily decompose in light, and require repeated application to maintain their algae-suppressing effect, resulting in high costs, cumbersome operation, and poor persistence of algae-suppressing action. Furthermore, the use of plant-derived terpenoid allelochemicals (such as menthol) alone, due to their relatively low algae-suppressing activity, requires high concentrations to achieve the desired algae-suppressing effect, leading to high practical application costs and hindering their widespread adoption in large-scale water treatment. Summary of the Invention

[0005] The purpose of this invention is to provide an environmentally friendly algae remover to solve the problems of the prior art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: An environmentally friendly algaecide, wherein the active ingredient of the environmentally friendly algaecide is compounded by diecryldimethylammonium chloride or polyhexamethylene biguanide hydrochloride and synergist menthol in a mass ratio of 1-100:100-1.

[0007] Furthermore, the mass ratio of the diecryldimethylammonium chloride to menthol is 1-20:5-1.

[0008] Furthermore, the mass ratio of the polyhexamethylene biguanide hydrochloride to menthol is 1-9:9-1.

[0009] Furthermore, the active ingredient accounts for 0.1-80% of the total mass of the environmentally friendly algaecide, with the remainder being permitted auxiliary ingredients.

[0010] Furthermore, the active ingredient accounts for 5-45% of the total mass of the environmentally friendly algaecide.

[0011] The present invention also provides the application of the environmentally friendly algaecide in inhibiting algae growth.

[0012] Furthermore, the algae include Chlorella ( Chlorella vulgaris ) and Microcystis aeruginosa ( Microcystis aeruginosa ).

[0013] Compared with the prior art, the present invention has the following beneficial effects: This invention scientifically combines dedecyl dimethyl ammonium chloride or polyhexamethylene biguanide hydrochloride with the natural terpene compound menthol. The synergistic effect between the two active ingredients was determined using the co-toxicity coefficient method. The results showed that when the mass ratio of dedecyl dimethyl ammonium chloride to menthol was 1-5:20-1 and the mass ratio of polyhexamethylene biguanide hydrochloride to menthol was 1-9:9-1, the co-toxicity coefficient of the combination was greater than 120, indicating that the combination's inhibitory effect on algae was significantly better than that of the individual agents used alone. Furthermore, menthol, as a natural plant-derived synergist, possesses certain algae-inhibiting activity and can enhance the algae-inhibiting activity of dedecyl dimethyl ammonium chloride or polyhexamethylene biguanide hydrochloride, helping to reduce the dosage while ensuring algae control efficacy, thereby effectively mitigating the risks associated with using quaternary ammonium compounds for algae control. Detailed Implementation

[0014] The technical solution of this invention patent will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention.

[0015] 1. Materials and Methods 1.1 Test algae Chlorella (Freshwater Algae Bank, Institute of Hydrobiology, Chinese Academy of Sciences, No. FACHB-2338) and Microcystis aeruginosa (Freshwater Algae Bank, Institute of Hydrobiology, Chinese Academy of Sciences, No. FACHB-905) were cultured in BG-11 medium, and scale-up culture was carried out before the experiment.

[0016] 1.2 Test Culture Medium See Table 1-2.

[0017] Table 1. BG-11 Culture Medium Formulation

[0018] Table 2. Formula for A5 Trace Element Solution

[0019] 1.3 Test reagents 95% Didecyl dimethyl ammonium chloride, 98% Polyhexamethylene biguanide hydrochloride, 98% Menthol (Shanghai Yuanye Biotechnology Co., Ltd., Product No. B21886) 1.4 Test Methods (Refer to "Guidelines for Indoor Bioassay Testing of Pesticides - Herbicides Part 10: Assay Tests for Photosynthetic Inhibitor Herbicides - Chlorella Method") After the test reagent is dissolved, it is diluted with 0.05% Tween-80 aqueous solution to prepare a high-concentration single-agent stock solution of 5000 mg / L, and multiple formulations are set up.

[0020] The tested algae were inoculated into BG-11 medium, sealed with sealing film, and pre-cultured for 7 days at 25℃, under continuous light at 5000 lx, and with rotating vibration at 100 r / min. A certain amount of the pre-cultured algal solution was then inoculated into 15 mL of BG-11 medium to achieve an initial algal cell concentration of 8 × 10⁻⁶ cells / mL. 5 The test solution was added to the above system to create five mass concentration gradients for each single-agent stock solution and each group of mixed solutions. A blank control was set up with no added reagent. The culture medium was then incubated for 4 days at 25℃, 5000 lx continuous light, and 100 r / min rotation. Using BG-11 medium as a reference solution, the absorbance of the algal solution at 680 nm was measured under a microscope using a hemocytometer. A linear regression equation between algal cell concentration and absorbance was established to calculate the growth inhibition rate of different treatments on algal cell concentration.

[0021]

[0022] 1.5 Data Analysis Regression analysis was performed on the logarithmic values ​​of drug concentrations and the probability values ​​of inhibition rates to calculate the ED of each treatment drug. 50 The co-toxicity coefficient (CTC value) of the mixture was calculated using the Sun Yunpei method.

[0023] The type of action of drug combination was evaluated based on the co-toxicity coefficient (CTC): CTC≤80 indicates antagonistic effect, 80<CTC<120 indicates additive effect, and CTC≥120 indicates synergistic effect. The results are shown in Table 3-4.

[0024] Table 3. Effects of the combination of didecyl dimethyl ammonium chloride and menthol on Chlorella vulgaris

[0025] As shown in Table 3, when the mass ratio of diecryldimethylammonium chloride to menthol is 1-20:5-1, the co-toxicity coefficient of Chlorella is 120.859-161.103, which is greater than 120, indicating a synergistic effect.

[0026] Table 4. Effects of polyhexamethylene biguanide hydrochloride combined with menthol on Microcystis aeruginosa

[0027] As shown in Table 4, when the mass ratio of polyhexamethylene biguanide hydrochloride to menthol is 1-9:9-1, the co-toxicity coefficient of Microcystis aeruginosa is 125.419-311.427, which is greater than 120, indicating a synergistic effect.

[0028] In summary, this invention scientifically combines decanted dimethyl ammonium chloride or polyhexamethylene biguanide hydrochloride with the natural terpene compound menthol. The synergistic effect between the two active ingredients was determined using the co-toxicity coefficient method. The results showed that when the mass ratio of decanted dimethyl ammonium chloride to menthol was 1-5:20-1 and the mass ratio of polyhexamethylene biguanide hydrochloride to menthol was 1-9:9-1, the co-toxicity coefficient of the combination was greater than 120, indicating that the combination's inhibitory effect on algae was significantly better than that of any single agent used alone. Furthermore, menthol, as a natural plant-derived synergist, possesses certain algae-inhibiting activity and can enhance the algae-inhibiting activity of decanted dimethyl ammonium chloride or polyhexamethylene biguanide hydrochloride, helping to reduce the dosage while ensuring algae control efficacy, thereby effectively mitigating the risks associated with using quaternary ammonium compounds for algae control.

[0029] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. An environmentally friendly algaecide, characterized in that, The active ingredient of the environmentally friendly algaecide is a compound of diecryldimethylammonium chloride or polyhexamethylene biguanide hydrochloride and menthol as a synergist in a mass ratio of 1-100:100-1.

2. The environmentally friendly algaecide according to claim 1, characterized in that, The mass ratio of diecryldimethylammonium chloride to menthol is 1-20:5-1.

3. The environmentally friendly algaecide according to claim 1, characterized in that, The mass ratio of polyhexamethylene biguanide hydrochloride to menthol is 1-9:9-1.

4. The environmentally friendly algaecide according to claim 1, characterized in that, The active ingredient accounts for 0.1-80% of the total mass of the environmentally friendly algaecide, with the remainder being permitted auxiliary ingredients.

5. The environmentally friendly algaecide according to claim 1, characterized in that, The active ingredient accounts for 5-45% of the total mass of the environmentally friendly algaecide.

6. The application of the environmentally friendly algaecide according to any one of claims 1-5 in inhibiting algae growth.

7. The application according to claim 6, characterized in that, The algae include Chlorella ( Chlorella vulgaris ) and Microcystis aeruginosa ( Microcystis aeruginosa ).

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