A salt- and drought-tolerant sorghum seed initiator, a salt- and drought-tolerant compound sorghum seed initiation coating agent, and an initiation coating method.

Abstract

This invention discloses a salt- and drought-tolerant sorghum seed initiator, a salt- and drought-tolerant composite sorghum seed initiating coating agent, and an initiation and coating method. The salt- and drought-tolerant sorghum seed initiator comprises the following raw material components: initiator including Bacillus thuringiensis 5.5-6.0 g / L, allicin 0.15-0.25 g / L, sugar-vinegar solution 2.0-2.5 g / L, brassinolide 0.5-0.8 g / L, sanguisorbin 27.69-31.54 mg / L, beauveria bassiana 0.01-0.1 g / L, and anhydrous betaine 0.03-0.05 g / L. This invention enhances seed vigor and replenishes seed nutrition. Through organic matter and physical barriers, it improves seed disease and insect resistance, effectively reduces the risk of cross-infection and external environmental contamination of seeds during production, processing, and inspection, and inhibits the threat of harmful pathogens in the soil environment after sowing. It is of great significance for improving seed germination rate, ensuring uniform and strong seedlings, reducing field sowing amount, controlling seed diseases, and protecting the environment. Moreover, it has outstanding salt and drought resistance and can be promoted to arid and saline-alkali areas.

Description

Technical Field

[0001] This invention relates to a salt- and drought-tolerant sorghum seed initiator, a salt- and drought-tolerant composite sorghum seed initiating coating agent, and an initiation coating method, belonging to the technical field of sorghum seed initiating coating agents. Background Technology

[0002] Sorghum (Sorghum bicolour L. Moench) is the fifth most common grain globally, cultivated in over 90 countries. Due to its excellent adaptability, sorghum is widely grown in harsh soils beyond fertile soils, particularly saline-alkali lands unsuitable for most other crops. Sorghum has a wide range of uses, serving not only as food for humans and animals but also as feed, brewing, and a raw material for new energy development. Statistics show that the annual sorghum planting area in Guizhou Province is approximately 4 million mu (about 667,000 hectares). Surveys in major sorghum-producing areas such as Renhuai City, Tongzi County, and Xishui County in Guizhou Province indicate that 80-90% of sorghum planting in Guizhou is direct seeding. However, due to the poor seed emergence ability of sorghum and its inability to withstand low temperatures during the seedling stage, the germination rate of direct seeding is less than 25%. To ensure uniform seedling emergence, the sowing rate must be increased, with the sowing rate per hill being 3-4 times the number of seedlings, reaching 20,000-30,000 seeds per mu (about 0.067 hectares). In actual production, this leads to seed waste, increased thinning costs, and an increase in seed-borne and soil-borne diseases. Furthermore, drought and high-salinity geological conditions can severely affect germination, leading to reduced yields. Summary of the Invention

[0003] This invention provides a salt- and drought-tolerant sorghum seed initiator, a salt- and drought-tolerant compound sorghum seed initiation coating agent, and an initiation coating method. The compound initiation promotes seeds to reach the pre-germination level, which is of great significance for improving seed germination rate, ensuring uniform and strong seedlings, reducing field sowing amount, seed diseases, reducing the rate of underground pests, biological control, and environmental protection. At the same time, it also significantly improves the salt and drought tolerance of sorghum seeds.

[0004] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is as follows:

[0005] A salt- and drought-tolerant sorghum seed initiator, comprising the following raw material components: initiator including Bacillus thuringiensis 5.5–6.0 g / L, allicin 0.15–0.25 g / L, sugar-vinegar solution 2.0–2.5 g / L, brassinolide 0.5–0.8 g / L, sanguisorbin 27.69–31.54 mg / L, beauveria bassiana 0.01–0.1 g / L, and anhydrous betaine 0.03–0.05 g / L.

[0006] Through experiments, the inventors discovered that the aforementioned initiators have significant effects on promoting germination and resisting adverse conditions. The inventors analyzed the reasons for this: Bacillus thuringiensis can secrete insecticidal proteins to reduce seed damage from pests during the seedling stage; allicin can eliminate pathogens (such as Fusarium and Pythium) on the seed surface and surrounding soil, reducing the risk of seed rot, activating seed metabolic enzyme activity, breaking dormancy, and promoting starch hydrolysis into soluble sugars to provide energy for germination; the sugar-vinegar solution can inhibit the reproduction of mid-term pathogens, provide carbon sources and trace elements (iron, zinc, etc.), and promote early seed energy metabolism; brassinolide, along with... It accelerates the decomposition of stored substances in the seed, providing energy for germination; sanguisorbin has strong antibacterial activity (especially against Gram-positive bacteria and fungi), which can prevent seed rot; its antioxidant properties reduce oxidative damage during germination, maintain cell membrane integrity, and ensure normal embryonic development; the indoleacetic acid (IAA) substances secreted by Beauveria bassiana stimulate root hair formation and expand the absorption area; it synergistically enhances the activity of antioxidant enzymes such as SOD and POD, improving stress resistance; betaine, as an osmotic regulator, maintains cell osmotic balance in the early stage of seed germination and prevents germination arrest caused by dehydration stress. The above substances exhibit a significant synergistic effect. Beauveria bassiana secretes chitinase to dissolve fungal cell walls, while Bacillus thuringiensis insecticidal proteins target soil pests, achieving "dual killing of insects and fungi." Ethylene trioxide disrupts the cell membranes of pathogenic bacteria, and sanguisorbin penetrates the bacterial cells to inhibit nucleic acid synthesis, thus doubly blocking fungal / bacterial reproduction (with an efficacy increase of over 50%). Ethylene trioxide and brassinolide accelerate germination. Bacillus thuringiensis, Beauveria bassiana, and sugar-vinegar solution improve seed microbial structure while inhibiting the growth of alkaliphilic pathogens such as Fusarium. Betaine, as a neutral active substance, significantly promotes the effects of sanguisorbin, Beauveria bassiana, and brassinolide, and also increases germination speed under saline-alkali or drought conditions. The comprehensive use of this compound initiator improves seed germination rate, ensures uniform seed emergence, prevents seed-borne diseases, and inhibits insect galls and eggs.

[0007] In order to better promote the dispersion of each component and improve the initiation effect, the solvent used in the above-mentioned salt- and drought-resistant sorghum seed initiator is a mixture of water, anhydrous ethanol and anhydrous methanol in a volume ratio of 80:(12-16):(8-4).

[0008] To enhance the initiation effect, the above-mentioned sweet and sour solution is prepared by mixing brown sugar and acetic acid at a ratio of (2-4) g: 1 ml. The sweet and sour solution of this application primarily serves to provide nutrition and promote initiation when combined with other substances. If utilizing the chemotaxis of pests, water and white wine would need to be added; however, this application does not serve this purpose, and therefore, water and white wine are not required when preparing the sweet and sour solution.

[0009] A salt- and drought-tolerant composite sorghum seed initiator coating agent, the raw material components of which include: the above-mentioned salt- and drought-tolerant sorghum seed initiator, seed coating agent and filler;

[0010] Seed coating agents include selenium pellets, alcohol, lime sulfur mixture, and organic pigment; among which, the amount of alcohol used is 35-45 ml per gram of selenium pellets, and the volume ratio of alcohol, lime sulfur mixture, and organic pigment is 195:(460-500):(300-340).

[0011] The filler is a mixture of limestone powder, zeolite powder, talc powder and bentonite in a mass ratio of 850:210:(1890~2090):(715~815).

[0012] The initiation treatment in this application results in faster and more uniform seed germination, providing a more homogeneous starting material for subsequent coating and pelleting treatments, and improving the seed's tolerance to adverse environments such as low temperature, drought, and salinity. Initiation-treated seeds ensure effective adhesion of the coating material and maintain their physical integrity during pelleting. The coating treatment protects the initiated, physiologically active but potentially "delicate" seeds, enabling them to resist early invasion by soil-borne diseases and underground pests, compensating for the time-limited effect of initiation treatments. Simultaneously, the coating directly delivers trace amounts of highly effective active substances to the microenvironment surrounding the seed / root system. The thin film layer formed by the coating also provides a more uniform and adhesive base surface, facilitating the adhesion and formation of subsequent pelleting materials. Pelleting encapsulates the coated seeds, providing additional physical protection, reducing wear during handling and sowing, and helping to maintain the stability and slow-release properties of the coating's active ingredients, providing more comprehensive initiation support. In summary, initiation treatment activates the seed's inherent potential, coating provides chemical protection and initiation support, and pelleting optimizes physical morphology, ensures precise sowing, and integrates functions. Only when these three processes work in harmony can the value of the seed be maximized.

[0013] To further enhance the seed treatment effect, the seed coating agent also includes 6-benzylaminopurine, and the mass ratio of selenium pellets to 6-benzylaminopurine is 2:(0.5-1).

[0014] The volume-to-mass ratio of the above-mentioned seeds, salt- and drought-tolerant sorghum seed initiator, seed coating agent and filler is 1000g:(8000~10000)ml:(10~15)ml:(2950~3150)g.

[0015] A composite initiation coating method for salt- and drought-tolerant sorghum seeds includes the following steps:

[0016] 1) Soak sorghum seeds in the salt-tolerant and drought-resistant sorghum seed initiator according to any one of claims 1-3 at a temperature of 20-25℃ for 3-6 hours;

[0017] 2) Dry the seeds obtained in step 1) at 35-40℃ until the moisture content is 10-13%;

[0018] 3) Add selenium pellets to alcohol and stir at room temperature (200-300 rpm) for 25-35 minutes; then add lime sulfur mixture, organic pigment and water in sequence, and mix using a vascular jet high shear mixer at 2800-3200 rpm for 25-30 minutes to obtain the seed coating agent, which should be stored at 4℃; the mass-volume ratio of selenium pellets, alcohol, lime sulfur mixture and organic pigment is 5g:(180-220)ml:(460-500)ml:(300-340)ml; the amount of water added is the amount of alcohol lost during the stirring process;

[0019] 4) Pour the seeds obtained in step 2) into a coating machine, add the seed coating agent obtained in step 3), stir at a speed of 25-35 r / min for 10-15 min, and then dry in a constant temperature electric drying oven at 35-40℃ for 20-30 min; wherein, the amount of seed coating agent used is 10-15 ml per kilogram of seeds;

[0020] 5) Take limestone powder, zeolite powder, talc powder and bentonite in a mass ratio of 850:210:(1890~2090):(715~815), pour them into a coating machine, set the speed to 25~30r / min, mix for 10~15min to obtain the filler;

[0021] 6) Pour the seeds obtained in step 4) into a coating machine, set the speed to 25-30 r / min, add the filler, binder, film-forming agent and dye obtained in step 5), stir for 15-25 min, dry in a constant temperature electric drying oven at 35-40℃ for 1-2 h, pack into a sealed seed bag, and store in a seed storage cabinet at 4℃.

[0022] The mass-volume ratio of seeds, salt- and drought-tolerant sorghum seed initiator, seed coating agent, filler, binder, film-forming agent and dye is 1000g:(8000~10000)ml:(10~15)ml:(2950~3150):(280~350):(15~35)(80~120).

[0023] The active substances in the initiator of this application can inhibit pathogenic fungi on the surface and inside of seeds, while promoting seed germination rate and enhancing stress resistance. Furthermore, suitable seed coating agents and fillers for sorghum seeds are screened, and an adhesive is used to seal the coated pelleted seeds. This creates a double-layer barrier between the seeds and external pathogens, containing minerals and an adhesive, effectively inhibiting infection by external pathogens while ensuring the slow release of the initiator and improving its utilization rate. This compound sorghum seed initiation coating is a further treatment after seed production, processing, and inspection. It can effectively alleviate the degree of pathogen infection during harvesting, threshing, and drying, prevent the spread of seed-borne diseases, reduce cross-infection between seeds after production, processing, and inspection, and reduce the threat of harmful pathogens in the soil environment after sowing.

[0024] In order to further improve the seed treatment effect, in step (3) above, 6-benzylaminopurine and selenium pellets are added to alcohol together, and the mass ratio of selenium pellets to 6-benzylaminopurine is 2:(0.5~1).

[0025] This application applies to the treatment of sorghum seeds, and particularly to the treatment of red-tasseled sorghum seeds.

[0026] The organic pigment added in step 3) serves two purposes: first, it acts as a carrier to dissolve the agent and allow it to adhere to the seeds; second, it serves as an identification agent, making it easier to distinguish from the induced seeds.

[0027] Unless otherwise specified, all percentages in this application are percentages by mass.

[0028] The sorghum seeds used in step 1) above are red-tasseled sorghum seeds.

[0029] Any techniques not mentioned in this invention are based on existing technologies.

[0030] This invention discloses a salt- and drought-tolerant compound sorghum seed initiation coating agent and its initiation coating method. Through the combination of specific compound initiators and coating agents, seed vigor is enhanced, seed nutrition is supplemented, and the disease and insect resistance of seeds is improved through organic matter and physical barriers. It effectively reduces the risk of cross-infection and external environmental contamination of seeds after production, processing, and inspection, and inhibits the threat of harmful pathogens in the soil environment after sowing. It is of great significance for improving seed germination rate, ensuring uniform and strong seedlings, reducing field sowing amount, controlling seed diseases, and protecting the environment. Moreover, it has outstanding salt and drought resistance and can be promoted to arid and saline-alkali areas. Attached Figure Description

[0031] Figure 1 This is a flowchart illustrating the preparation process of the salt- and drought-resistant composite sorghum seed initiating coating agent of the present invention; in the flowchart, green represents the raw material components;

[0032] Figure 2 Figure 1 shows untreated seeds (left, blank) and seeds treated with the composite initiator-induced coated pelleting sorghum seeds of the present invention (right, treated in Example 1).

[0033] Figure 3 Comparison of treated seeds (A) and untreated seeds (blank B) after 7 days of germination in Example 1 of this invention;

[0034] Figure 4 Comparative images of treated seeds (A) and untreated seeds (blank B) after 7 days of hydroponics in Example 1 of this invention;

[0035] Figure 5 A comparison chart of treated seeds (A) and untreated seeds (blank B) at 14 days after germination in Example 1 of this invention; Detailed Implementation

[0036] To better understand the present invention, the following embodiments further illustrate the content of the present invention, but the content of the present invention is not limited to the following embodiments.

[0037] Unless otherwise specified, all cases were conducted at room temperature (15–25°C).

[0038] The sorghum seeds (commercial seeds were sampled and tested using the quartering method after cleaning, impurity removal, and mixing) were provided by Moutai Group Hongyingzi Agricultural Technology Development Co., Ltd.

[0039] Moisture content was measured using a grain moisture analyzer.

[0040] Bacillus thuringiensis, FS suspension concentrate, Shandong Lukang Biological Pesticide Co., Ltd., 8000 IU / ul; Ethylene, EC emulsifiable concentrate, Kaifeng Dadi Agricultural Chemical Biotechnology Co., Ltd., 80% purity; Sweet and sour liquid, made by mixing brown sugar and acetic acid (commercial food-grade brown sugar and red vinegar, with acetic acid content of 5% in the red vinegar, calculated as acetic acid) at a ratio of 3g:1ml; Brassinolide, AS aqueous solution, Chengdu Xinchaoyang Crop Science Co., Ltd., 0.01% active ingredient; Sanguisorbide, Henan Shenlan Food Ingredients Co., Ltd., 60% purity; Beauveria bassiana, 10 billion / g, Beihai Qiangxing Biotechnology Co., Ltd.; Anhydrous betaine, Shandong Yousuo Chemical Technology Co., Ltd., 98% purity.

[0041] Lime sulfur mixture, Yibin Chuan'an Gaoke Pesticide Co., Ltd., content 29%; Selenium pellets, Shanghai Maclean Biochemical Technology Co., Ltd., purity 99.90%; 6-Benzylaminopurine, Shanghai Yuanye Biotechnology Co., Ltd., purity 99%. Organic pigment, H009, Hangzhou Hongjing Coatings Co., Ltd.

[0042] Limestone powder, Henan Borun Foundry Materials Co., Ltd., 1000 mesh; Zeolite powder, Shijiazhuang Huabang Mineral Products Co., Ltd., 1250 mesh; Talc powder, Shanghai Fengxian Bazishi Powder Factory, 1250 mesh; Bentonite, Henan Borun Foundry Materials Co., Ltd., 1000 mesh.

[0043] Binder, KA200, Green New Fujian Food Co., Ltd.; Film-forming agent, HC19, Shenzhen Hangcai Pigment Co., Ltd.; Dyeing agent, HC19, Shenzhen Hangcai Pigment Co., Ltd.

[0044] Example 1

[0045] A method for preparing a salt- and drought-tolerant composite sorghum seed initiating coating agent includes the following steps:

[0046] 1) Soak 1000g of red sorghum seeds in an initiator solution at 20℃ for 6 hours; wherein, the initiator solution components include: Bacillus thuringiensis 6.0g / L, allicin 0.2g / L, sugar-vinegar solution 2.2g / L, brassinolide 0.6g / L, sanguisorbin 29.31mg / L, beauveria bassiana 0.05g / L, anhydrous betaine 0.04g / L, and solvent 10L (pure water, anhydrous ethanol and anhydrous methanol in a volume ratio of 80:15:6);

[0047] 2) Dry the seeds obtained in step 1) at a constant temperature of 40℃ for 9 hours until the moisture content is 12%.

[0048] 3) Take 5g of selenium capsules and add 195ml of alcohol. Stir magnetically (200r / min) for 35 minutes at room temperature. Then add 480ml of lime-sulfur mixture, 320ml of organic pigment, and pure water to a final volume of 1000ml (to compensate for the evaporation of alcohol during stirring). Mix using a vascular jet high-shear mixer at 3000r / min for 30 minutes and store at 4℃.

[0049] 4) Pour the seeds obtained in step 2) into the coating machine, set the speed to 30 r / min, add 12.5 ml of the seed coating agent obtained in step 3), stir for 15 min, and the surface of all sorghum seeds is evenly coated with the agent. Then dry in a constant temperature electric drying oven at 40℃ for 30 min.

[0050] 5) Take 850g of limestone powder, 210g of zeolite powder, 2000g of talc powder and 750g of bentonite, pour them into the BY-300 coating machine, set the speed to 30r / min, mix for 15min to obtain the filler.

[0051] 6) Pour the seeds obtained in step 4) into a BY-300 coating machine, set the speed to 30 r / min, add the filler, binder, film-forming agent, and dye obtained in step 5) 300g, stir for 25 min, until the seeds are evenly coated, uniform in size, and have a smooth surface. Dry in a constant temperature electric drying oven at 40℃ for 2 h, pack into sealed seed bags, and store in a seed storage cabinet at 4℃.

[0052] Example 2

[0053] The difference from Example 1 is that 2g of 6-benzylaminopurine was added to the seed coating agent in this example. 6-Benzylaminopurine was added to the alcohol along with the selenium pellets in step 3). All other steps were the same as in Example 1.

[0054] Example 3

[0055] The difference from Example 1 is that in step 3), the selenium pellets are omitted. All other steps are the same as in Example 1.

[0056] Example 4

[0057] The difference from Example 1 is that steps 3)-4) of the seed coating agent treatment are omitted. The rest are the same as in Example 1.

[0058] Example 5

[0059] The difference from Example 1 is that step 5) of zeolite powder is omitted. The rest is the same as in Example 1.

[0060] Comparative Example 1

[0061] The difference from Example 1 is that ethoxylated sorghum seed initiator in step 1) is omitted. All other steps are the same as in Example 1.

[0062] Comparative Example 2

[0063] The difference from Example 1 is that the sugar-vinegar solution was omitted in step 1) of the salt- and drought-tolerant sorghum seed initiator. All other steps are the same as in Example 1.

[0064] Comparative Example 3

[0065] The difference from Example 1 is that sanguinarine was omitted from the salt- and drought-tolerant sorghum seed initiator in step 1). All other steps are the same as in Example 1.

[0066] Comparative Example 4

[0067] The difference from Example 1 is that beauveria bassiana extract was omitted from the salt- and drought-tolerant sorghum seed initiator in step 1). All other steps are the same as in Example 1.

[0068] Comparative Example 5

[0069] The difference from Example 1 is that anhydrous betaine was omitted from the salt- and drought-tolerant sorghum seed initiator in step 1). All other steps are the same as in Example 1.

[0070] Application examples

[0071] Experimental Location: Indoor experiments were conducted in the laboratory of the Qiannan Academy of Agricultural Sciences. Unless otherwise specified, the following experiments were conducted in an artificial climate chamber with a temperature of 25℃, humidity of 50%, and a photoperiod of 14 hours of light followed by 10 hours of darkness, at a light intensity of 187 μmol / (m²). 2 ·s).

[0072] Germination experiment: Seeds from each treatment were sown in glass petri dishes containing three layers of filter paper (the filter paper was soaked in water until saturated). Each petri dish contained 30 seeds, with 10 petri dishes constituting one treatment, and the experiment was repeated three times. Germination rate and sprout length were measured on day 7. Figure 3 As shown in Table 1, the germination results are as follows.

[0073] Hydroponic Experiment: Seeds treated in Example 1 and control seeds (blank) were sown on perforated 96-well PCR plates. A float plate was placed on a plastic square box of equal area, allowing the PCR plate to float on the surface of the culture medium, which was Hogrange solution. 48 seeds were sown in each section. The hydroponic boxes were arranged completely randomly in a light incubator. Root length (underground part) and shoot length (above-ground part) were measured on day 7. Plant morphology is as follows. Figure 4 As shown, Figure 4 Image A shows the growth of the treated seeds (Example 1). Figure 4 In Figure B, the growth of the control seeds is shown. Compared to the control seeds, the above-ground parts grew by an average of 1.51 cm and the underground parts grew by an average of 0.62 cm.

[0074] Germination experiment: Sorghum seeds from each treatment were planted in flowerpots containing a 15cm layer of soil (yellow loam, 40% soil moisture), at a sowing depth of 3cm. Each flowerpot contained 15 seeds, with 10 flowerpots constituting one treatment, and the experiment was repeated three times. The germination rate (%) was measured on day 14 after sowing. Figure 4 As shown.

[0075] Salt stress experiment: Salt stress was simulated using NaCl solution at a concentration of 200 mmol / L. Seeds from each treatment were sown in glass petri dishes containing three layers of filter paper (saturated with NaCl solution). Each petri dish contained 30 seeds, with 10 petri dishes constituting one treatment, and the experiment was repeated three times. Germination potential was measured on day 4, and germination rate and sprout length were measured on day 7. Germination days, germination index, and vigor index were calculated. The results are shown in Table 2.

[0076] Drought stress experiment: Salt stress was simulated using a 20% PEG-6000 solution. The PEG-6000 solution was poured into hydroponic boxes, and dry seeds from each treatment were sown on perforated floating boards with independent sowing areas. The floating boards were secured on both sides with foam boards, allowing them to float on the surface of the PEG-6000 solution. 50 seeds were sown in each area, with 10 areas constituting one treatment, and the experiment was repeated three times. The hydroponic boxes were arranged completely randomly in a light incubator. Germination potential was measured on day 4, and germination rate and sprout length were measured on day 7. Germination days, germination index, and vigor index were calculated. The results are shown in Table 3.

[0077] Table 1 shows the germination rates of the composite sorghum seeds obtained in each case.

[0078]

[0079]

[0080] In the table above, seed germination is defined as the appearance of white seeds, and the number of germinations is recorded until the number remains constant; blank spaces indicate that no seed treatment was performed.

[0081] Table 2 shows the germination of the composite sorghum seeds obtained in each case under salt stress.

[0082] Example Germination rate Germination potential Germination Index Vitality Index Germination days bud growth Example 1 96.33 91.16 36.83 292.06 4.15 7.93 Example 2 96.00 92.03 36.65 283.67 4.13 7.74 Example 3 88.33 85.06 34.18 247.98 4.42 7.26 Example 4 84.33 84.33 31.75 233.36 4.53 7.35 Example 5 90.66 87.83 34.52 250.36 4.38 7.25 blank 68.00 66.33 25.23 167.02 4.53 6.62 Comparative Example 1 76.33 73.66 27.60 185.75 4.09 6.73 Comparative Example 2 78.33 76.33 32.98 227.07 4.44 6.89 Comparative Example 3 72.00 70.66 24.17 163.15 4.09 6.75 Comparative Example 4 80.33 78.16 33.00 228.03 4.33 6.91 Comparative Example 5 82.33 81.33 34.03 233.79 4.18 6.87

[0083] In the table above, seed germination is defined as the appearance of white seeds, and the number of germinations is recorded until the number remains constant; blank spaces indicate that no seed treatment was performed.

[0084] Table 3 shows the germination of the composite sorghum seeds obtained in each case under drought stress.

[0085]

[0086]

[0087] In the table above, seed germination is defined as the appearance of white seeds, and the number of germinations is recorded until the number remains constant; blank spaces indicate that no seed treatment was performed.

Claims

1. A salt- and drought-tolerant sorghum seed initiator, characterized in that: Its raw material components include: initiator including Bacillus thuringiensis 5.5-6.0 g / L, allicin 0.15-0.25 g / L, sugar and vinegar solution 2.0-2.5 g / L, brassinolide 0.5-0.8 g / L, sanguisorbin 27.69-31.54 mg / L, beauveria bassiana 0.01-0.1 g / L, and anhydrous betaine 0.03-0.05 g / L.

2. The salt- and drought-tolerant sorghum seed initiator according to claim 1, characterized in that: The solvent used is a mixture of water, anhydrous ethanol and anhydrous methanol in a volume ratio of 80:(12-16):(8-4).

3. The salt- and drought-tolerant sorghum seed initiator according to claim 1 or 2, characterized in that: Sweet and sour sauce is made by mixing brown sugar and acetic acid in a ratio of (2-4)g:1ml.

4. A salt- and drought-tolerant composite sorghum seed initiator coating agent, characterized in that: Its raw material components include: the salt-tolerant and drought-resistant sorghum seed initiator, seed coating agent and filler as described in any one of claims 1-3; Seed coating agents include selenium pellets, alcohol, lime sulfur mixture, and organic pigment; among which, the amount of alcohol used is 35-45 ml per gram of selenium pellets, and the volume ratio of alcohol, lime sulfur mixture, and organic pigment is 195:(460-500):(300-340). The filler is a mixture of limestone powder, zeolite powder, talc powder and bentonite in a mass ratio of 850:210:(1890~2090):(715~815).

5. The salt-tolerant and drought-resistant composite sorghum seed initiator coating agent according to claim 4, characterized in that: The seed coating agent also includes 6-benzylaminopurine, and the mass ratio of selenium pellets to 6-benzylaminopurine is 2:(0.5-1).

6. The salt-tolerant and drought-resistant composite sorghum seed initiator coating agent according to claim 4 or 5, characterized in that: The volume-to-mass ratio of seeds, salt- and drought-tolerant sorghum seed initiator, seed coating agent and filler is 1000g:(8000~10000)ml:(10~15)ml:(2950~3150)g.

7. A composite initiation coating method for salt- and drought-tolerant sorghum seeds, characterized in that: Includes the following steps: 1) Soak sorghum seeds in the salt-tolerant and drought-resistant sorghum seed initiator according to any one of claims 1-3 at a temperature of 20-25℃ for 3-6 hours; 2) Dry the seeds obtained in step 1) at 35-40℃ until the moisture content is 10-13%; 3) Add selenium pellets to alcohol and stir at room temperature for 25-35 minutes; then add lime sulfur mixture and organic pigment in sequence, and mix using a vascular jet high shear mixer at 2800-3200 r / min for 25-30 minutes to obtain seed coating agent, which is stored at 4℃; wherein, the mass-volume ratio of selenium pellets, alcohol, lime sulfur mixture and organic pigment is: 5g:(180-220)ml:(460-500)ml:(300-340)ml; 4) Pour the seeds obtained in step 2) into a coating machine, add the seed coating agent obtained in step 3), stir at a speed of 25-35 r / min for 10-15 min, and then dry at 35-40℃ for 20-30 min; wherein, the amount of seed coating agent used is 10-15 ml per kilogram of seeds; 5) Take limestone powder, zeolite powder, talc powder and bentonite in a mass ratio of 850:210:(1890~2090):(715~815), pour them into a coating machine, set the speed to 25~30r / min, mix for 10~15min to obtain the filler; 6) Pour the seeds obtained in step 4) into a coating machine, set the speed to 25-30 r / min, add the filler, binder, film-forming agent and dye obtained in step 5), stir for 15-25 min, dry at 35-40℃ for 1-2 h, pack into a sealed seed bag and store in a seed storage cabinet at 4℃. The mass-volume ratio of seeds, salt- and drought-tolerant sorghum seed initiator, seed coating agent, filler, binder, film-forming agent and dye is 1000g:(8000~10000)ml:(10~15)ml:(2950~3150):(280~350):(15~35)(80~120).

8. The composite initiation coating method for salt-tolerant and drought-resistant sorghum seeds according to claim 7, characterized in that: In step (3), 6-benzylaminopurine and selenium capsules are added to alcohol together, with the mass ratio of selenium capsules to 6-benzylaminopurine being 2:(0.5-1).

9. The composite initiation coating method for salt-tolerant and drought-resistant sorghum seeds according to claim 7 or 8, characterized in that: In step 1), the sorghum seeds are red-tasseled sorghum seeds.

Images