A shampoo containing Poria cocos peel extract and its preparation method

Abstract

This invention discloses a shampoo containing Poria cocos peel extract and its preparation method, belonging to the field of daily necessities technology. Poria cocos peel extract, rich in triterpenoids (such as pachymic acid), can effectively inhibit Malassezia furfur, eliminating dandruff at its source. The shampoo of this invention adds Poria cocos peel extract, piroctone olamine salt, and zinc lactate, which synergistically enhance the effects, complementing antibacterial and scalp microecological regulation. It provides excellent dandruff removal, anti-itch, and oil control effects while being gentle and non-irritating, and is less prone to drug resistance with long-term use. The raw materials are derived from waste Poria cocos peel, maximizing resource utilization and ensuring quality control. Furthermore, the shampoo of this invention is formulated with anionic surfactants, amphoteric surfactants, nonionic surfactants, and various conditioning agents, combining excellent foaming and cleaning with repairing and smoothing effects, achieving integrated dandruff removal and hair care.

Description

Technical Field

[0001] This invention relates to the field of daily necessities technology, and more specifically, to a shampoo containing Poria cocos peel extract and its preparation method. Background Technology

[0002] Seborrheic dermatitis (SD) is a chronic, relapsing inflammatory skin disease that primarily occurs in areas rich in sebaceous glands, such as the scalp, face, chest, and back. Epidemiological surveys show that the prevalence of SD is increasing globally, and the affected population is becoming increasingly younger. Current research suggests that the pathogenesis of seborrheic dermatitis is closely related to the excessive proliferation of Malassezia, abnormal sebum secretion, impaired skin barrier function, and immune inflammatory responses. Malassezia breaks down triglycerides in sebum into free fatty acids through secreted lipases, stimulating keratinocytes and immune cells to release pro-inflammatory factors such as TNF-α, IL-6, and IL-1β, inducing and maintaining an inflammatory response, leading to symptoms such as dandruff.

[0003] Currently, most commercially available anti-dandruff shampoos rely on chemically synthesized anti-dandruff agents, such as ketoconazole, piroctone olamine (OCT), and zinc pyrithione (ZPT). For example, Chinese patent application CN202310308934.0 discloses a compound ketoconazole shampoo with multiple antifungal properties and its preparation method, using miconazole nitrate, clobetasol propionate, and ciclopirox olamine as the main antifungal raw materials. However, ketoconazole antifungal drugs have problems such as easy relapse after discontinuation and local adverse reactions with long-term use. Another example is Chinese patent application CN201811149530.7, which discloses a mild anti-dandruff and anti-itch composition, using piroctone olamine and auxiliary anti-dandruff ingredients. However, long-term use of existing chemical anti-dandruff agents may cause side effects such as scalp irritation and hair damage, and some anti-dandruff agents have been restricted in use in many countries and regions due to safety concerns.

[0004] Furthermore, with the increasing demand for natural herbal hair care products, anti-dandruff active ingredients derived from traditional Chinese medicine have received widespread attention, and more products with traditional Chinese medicine active ingredients are gradually appearing on the market. For example, Chinese patent application number CN201410436738.2 discloses an anti-dandruff and anti-itch shampoo, whose raw materials include traditional Chinese medicine ingredients such as Smilax glabra, claiming to have good anti-dandruff and anti-itch effects. Another example is Chinese patent application number CN201811524112.1, which discloses a traditional Chinese medicine shampoo and its preparation method. This traditional Chinese medicine shampoo contains ingredients such as ginger, tea seed, Polygonum multiflorum, wild chrysanthemum, Platycladus orientalis leaf, mulberry leaf, Eclipta prostrata, Salvia miltiorrhiza, Artemisia annua, Poria cocos, Angelica sinensis, Cnidium monnieri, leek seed, ginseng, clove, peppermint oil, and camellia oil. Therefore, current technologies merely combine a large number of traditional Chinese medicine ingredients to prepare shampoos without specifically adding effective active ingredients or specifically screening for the effective components of Poria cocos peel extract with anti-Malaisefelterus activity. In other words, the effective components are unclear, and the mechanism of action is also unclear, making it difficult to achieve precise application and the desired therapeutic and conditioning effects for seborrheic scalp dermatitis. Furthermore, there are few reports on the synergistic effects of Poria cocos peel extract in shampoo formulations. Summary of the Invention

[0005] In view of this, in order to solve one of the above-mentioned technical problems, the present invention provides a shampoo containing Poria cocos peel extract and its preparation method, the specific technical solution of which is as follows: A shampoo containing Poria cocos peel extract, the shampoo comprising Poria cocos peel extract containing triterpenoids, the triterpenoids including pachymic acid; and the amount of Poria cocos peel extract added to the shampoo by weight percentage is 0.05% to 5%.

[0006] Furthermore, the preparation method of the Poria cocos peel extract includes the following steps: Poria peel is pretreated to obtain pretreated coarse powder of Poria peel; Add coarse powder of Poria cocos peel to an ethanol solution with a mass percentage concentration of 60-90%, soak for 10-30 minutes, reflux extract for 30-60 minutes, filter, collect the filtrate, recover the ethanol under reduced pressure, concentrate, and obtain Poria cocos peel extract. The Poria cocos peel extract is added to water in a volume equivalent to 1 to 3 times that of the Poria cocos peel extract, and stirred to form a uniformly dispersed suspension to form an aqueous phase. The aqueous phase is first extracted with petroleum ether 1 to 3 times, and the aqueous phase is retained. Then it is extracted with dichloromethane 1 to 3 times, and the aqueous phase is retained. Then it is extracted with ethyl acetate 1 to 3 times. The ethyl acetate layer is collected, and the ethyl acetate is recovered under reduced pressure. After drying, the Poria cocos peel extract is obtained.

[0007] Furthermore, the coarse powder of Poria cocos peel is passed through a 20-60 mesh sieve; The ratio of the coarse powder of Poria cocos peel to the ethanol solution is 1g:6~10mL; The volume ratio of Poria cocos peel extract to petroleum ether is 1:(0.5~2.5); the volume ratio of Poria cocos peel extract to dichloromethane is 1:(0.5~2.5); the volume ratio of Poria cocos peel extract to ethyl acetate is 1:(0.5~2.5).

[0008] Furthermore, the shampoo also includes the following ingredients by weight percentage: synergist A 0.1%~0.5%, synergist B 0.3%~2%, surfactant 15%~30%, conditioning agent 0.5%~2.0%, moisturizer 1%~3%, fragrance 0.1%~1.0%, preservative 0.1%~1.0%, pH adjuster 0.1%~0.3%, chelating agent 0.1%~0.5%, thickener 0.1%~3%, and the balance being water.

[0009] Furthermore, the synergist A is pyrrolidone ethanolamine salt; the synergist B is zinc lactate.

[0010] Further, the surfactant is at least one of cocamidopropyl betaine, sodium lauryl ether sulfate, ammonium lauryl sulfate, sodium cocoyl methyl taurate, polyglycerol-10 lauryl ester, decyl glucoside, and lauryl ether-16.

[0011] Further, the conditioning agent is at least one of hydroxypropyl guar hydroxypropyltrimethylammonium chloride, polyquaternium salt-10, polyquaternium salt-22, polyquaternium salt-52, and amino-terminated polydimethylsiloxane.

[0012] Further, the humectant is 1,3-butanediol; the preservative is at least one of sodium benzoate, p-hydroxyacetophenone, and phenoxyethanol; the pH adjuster is citric acid; and the chelating agent is tetrasodium diacetate of glutamate.

[0013] Furthermore, the thickener is at least one of sodium chloride, cocamidomethyl MEA, hydrolyzed corn starch, hydroxypropyl cellulose, and hydroxypropyl methylcellulose.

[0014] In addition, the present invention also provides a method for preparing a shampoo containing Poria cocos peel extract, the preparation method comprising the following steps: S1. Hydroxypropyl guar gum, hydroxypropyl trimethylammonium chloride, and polyquaternium salt-10 were added to water and stirred until evenly dispersed. Then, tetrasodium diacetate of glutamate, sodium benzoate, and citric acid were added, and the mixture was heated and stirred until dissolved to obtain phase A. S2. Cocamidopropyl betaine, sodium lauryl ether sulfate, ammonium lauryl sulfate, sodium cocoyl methyl taurate, polyglycerol-10 lauryl ester, and stearamide-propyl dimethylamine lactate were added to water, heated and stirred to dissolve, and kept warm to obtain phase B. S3. Mix phase A and phase B evenly, cool down, add glycolipid, polyquaternium-22, polyquaternium-52, hydrolyzed corn starch, and piroctone ethanolamine salt, stir and treat to obtain mixture A; S4. After the mixture A is cooled to below 55°C, zinc lactate, decyl glucoside, PEG-40 hydrogenated castor oil, Poria cocos peel extract, 1,3-butanediol and amino-terminated polydimethylsiloxane are added to the mixture A, and the mixture is stirred. Then, phenoxyethanol and fragrance are added and stirred evenly to obtain mixture B. S5. Add cocamide methyl MEA and sodium chloride to the mixture B, and stir to obtain shampoo.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. The Poria cocos peel extract obtained by this invention contains abundant triterpenoids (including pachymic acid), which have excellent in vitro anti-Malassezia furfur inhibitory effects. It can effectively inhibit the excessive proliferation of Malassezia furfur, which is closely related to seborrheic dermatitis and dandruff, and play a role in removing dandruff from the root.

[0016] 2. This invention innovatively applies Poria cocos peel extract to the preparation system of a shampoo. Through scientific compounding with piroctone olamine salt and zinc lactate, a synergistic and gentle anti-dandruff and oil-controlling active system is constructed. Piroctone olamine salt, a recognized broad-spectrum antifungal agent, exerts its anti-dandruff effect by inhibiting lipid synthesis in the Malassezia cell membrane. Its mechanism of action is complementary to that of the triterpenoid active ingredients in Poria cocos peel extract, such as pachymic acid and dehydropachymic acid. The latter reduces abnormal dandruff by regulating the excessive proliferation pathway of scalp keratinocytes. The two work synergistically to block the core pathway of dandruff formation from different targets. Simultaneously, the added zinc lactate can alleviate scalp redness and itching by regulating the balance of scalp surface flora, inhibiting the excessive release of inflammatory factors such as TNF-α, and simultaneously assisting in regulating excessive sebum secretion. The combination of these three ingredients produces a significant synergistic effect, achieving superior triple efficacy of dandruff removal, itch relief, and oil control with far lower active ingredient levels than conventional formulas. Simultaneously, it significantly reduces the potential scalp irritation caused by single high-concentration dandruff-removing ingredients. Furthermore, as a natural plant-derived active ingredient, Poria cocos peel extract is unlikely to induce drug resistance in Malassezia with long-term use and has no significant skin irritation. It effectively avoids the side effects of dry scalp and compensatory dandruff that are easily caused by long-term use of ordinary anti-dandruff shampoos, making it particularly suitable for the daily cleansing and long-term care needs of people with sensitive scalps. Ultimately, this significantly improves the overall gentleness of the shampoo formula of this invention, balancing powerful efficacy with user comfort.

[0017] 3. The Poria cocos peel extract of this invention is derived from the outer skin of the traditional Chinese medicine Poria cocos. It is a functional raw material from a natural plant source. It reuses waste Poria cocos peel, maximizing resource utilization. Moreover, the quality from raw material to finished product is controllable, avoiding the technical defects of traditional Chinese medicine shampoos with complex ingredients and unclear pharmacodynamic material basis. It is also convenient for industrial production and quality control.

[0018] 4. The shampoo of this invention, based on the addition of Poria cocos peel extract, is scientifically formulated with anionic surfactants (sodium lauryl ether sulfate, ammonium lauryl sulfate), amphoteric surfactants (cocamidopropyl betaine), and nonionic surfactants (decyl glucoside, polyglycerol-10 laurate), giving the shampoo excellent foaming, cleansing, and gentleness. Simultaneously, the addition of multiple conditioning agents, including polyquaternium-10, polyquaternium-22, polyquaternium-52, and amino-terminated polydimethylsiloxane, effectively repairs damaged hair, reduces static electricity, and improves hair smoothness and shine, achieving the goals of dandruff removal and conditioning. Attached Figure Description

[0019] The invention will be further understood from the following description taken in conjunction with the accompanying drawings. The components in the drawings are not necessarily drawn to scale, but rather the emphasis is on illustrating the principles of the embodiments. In different views, the same reference numerals designate corresponding parts.

[0020] Figure 1 This is a schematic diagram of the pamoate standard curve in the experimental examples of this invention; Figure 2 This is a schematic diagram of the antibacterial test experiment of Malassezia furfur extract prepared in Example 1 of the present invention. Detailed Implementation

[0021] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to its embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and do not limit the scope of protection of the invention.

[0022] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to limit the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0023] According to one embodiment of the present invention, a shampoo containing Poria cocos peel extract is provided. The shampoo includes Poria cocos peel extract, which contains triterpenoids, including pachymic acid. The amount of Poria cocos peel extract added to the shampoo is 0.05% to 5% by mass percentage.

[0024] In one embodiment, the method for preparing the Poria cocos peel extract includes the following steps: Poria peel is pretreated to obtain pretreated coarse powder of Poria peel; Add coarse powder of Poria cocos peel to an ethanol solution with a mass percentage concentration of 60-90%, soak for 10-30 minutes, reflux extract for 30-60 minutes, filter, collect the filtrate, recover the ethanol under reduced pressure, concentrate, and obtain Poria cocos peel extract. The Poria cocos peel extract is added to water in a volume equivalent to 1 to 3 times that of the Poria cocos peel extract, and stirred to form a uniformly dispersed suspension to form an aqueous phase. The aqueous phase is first extracted with petroleum ether 1 to 3 times, and the aqueous phase is retained. Then it is extracted with dichloromethane 1 to 3 times, and the aqueous phase is retained. Then it is extracted with ethyl acetate 1 to 3 times. The ethyl acetate layer is collected, and the ethyl acetate is recovered under reduced pressure. After drying, the Poria cocos peel extract is obtained.

[0025] In one embodiment, the coarse powder of Poria cocos peel passes through a 20-60 mesh sieve; The ratio of the coarse powder of Poria cocos peel to the ethanol solution is 1g:6~10mL; The volume ratio of Poria cocos peel extract to petroleum ether is 1:(0.5~2.5); the volume ratio of Poria cocos peel extract to dichloromethane is 1:(0.5~2.5); the volume ratio of Poria cocos peel extract to ethyl acetate is 1:(0.5~2.5).

[0026] In one embodiment, an ethanol solution with a percentage concentration of 90% is prepared.

[0027] In one embodiment, the extraction reflux time is preferably 45 minutes.

[0028] In one embodiment, the shampoo further comprises the following ingredients by weight percentage: synergist A 0.1%~0.5%, synergist B 0.3%~2%, surfactant 15%~30%, conditioning agent 0.5%~2.0%, moisturizer 1%~3%, fragrance 0.1%~1.0%, preservative 0.1%~1.0%, pH adjuster 0.1%~0.3%, chelating agent 0.1%~0.5%, thickener 0.1%~3%, and the balance being water.

[0029] In one embodiment, the synergist A is pyrrolidone ethanolamine salt; and the synergist B is zinc lactate.

[0030] In one embodiment, the surfactant is at least one of cocamidopropyl betaine, sodium lauryl ether sulfate, ammonium lauryl sulfate, sodium cocoyl methyl taurate taurate, polyglycerol-10 lauryl ester, decyl glucoside, and lauryl ether-16.

[0031] In one embodiment, the conditioning agent is at least one of hydroxypropyl guar hydroxypropyltrimethylammonium chloride, polyquaternium-10, polyquaternium-22, polyquaternium-52, and amino-terminated polydimethylsiloxane.

[0032] In one embodiment, the humectant is 1,3-butanediol; the preservative is at least one of sodium benzoate, p-hydroxyacetophenone, and phenoxyethanol; the pH adjuster is citric acid; and the chelating agent is tetrasodium diacetate of glutamate.

[0033] In one embodiment, the thickener is at least one of sodium chloride, cocamidomethyl MEA, hydrolyzed corn starch, hydroxypropyl cellulose, and hydroxypropyl methylcellulose.

[0034] In addition, the present invention also provides a method for preparing a shampoo containing Poria cocos peel extract, the preparation method comprising the following steps: S1. Hydroxypropyl guar gum, hydroxypropyl trimethylammonium chloride, and polyquaternium salt-10 were added to water and dispersed evenly at high speed. Then, tetrasodium diacetate of glutamate, sodium benzoate, and citric acid were added, and the mixture was heated and stirred to dissolve, yielding phase A. S2. Cocamidopropyl betaine, sodium lauryl ether sulfate, ammonium lauryl sulfate, sodium cocoyl methyl taurate taurate, polyglycerol-10 lauryl ester, and stearamide-propyl dimethylamine lactate are added to water, heated and stirred to dissolve, and kept warm to obtain phase B. S3. Mix phase A and phase B evenly, cool down, add glycolipid, polyquaternium-22, polyquaternium-52, hydrolyzed corn starch, and piroctone ethanolamine salt, stir and treat to obtain mixture A; S4. After the mixture A is cooled to below 55°C, zinc lactate, decyl glucoside, PEG-40 hydrogenated castor oil, Poria cocos peel extract, 1,3-butanediol and amino-terminated polydimethylsiloxane are added to the mixture A, and the mixture is stirred. Then, phenoxyethanol and fragrance are added and stirred evenly to obtain mixture B. S5. Add cocamide methyl MEA and sodium chloride to the mixture B, and stir to obtain shampoo.

[0035] In one embodiment, in step S1, the rotational speed for high-speed uniform dispersion is 1000~2000 r / min.

[0036] In one embodiment, the conditions for heating, stirring and dissolving in step S1 are: temperature of 70~85℃, stirring speed of 500~1000r / min, and stirring time of 20~60min.

[0037] In one embodiment, the conditions for heating and stirring to dissolve in step S2 are: temperature of 80~85℃, stirring speed of 300~500r / min, stirring time of 20~60min; and holding at this temperature for 10~20min.

[0038] In one embodiment, in step S3, the temperature is lowered to below 60°C.

[0039] In one embodiment, the stirring conditions in step S3 are: a rotation speed of 100~500 r / min and a time of 10~30 min.

[0040] In one embodiment, the stirring conditions in step S4 are: a rotation speed of 100~500 r / min and a time of 10~20 min.

[0041] In one embodiment, the stirring conditions in step S5 are: a rotation speed of 100~500 r / min and a time of 10~15 min.

[0042] In one embodiment, the shampoo has a viscosity of 2000~5000 mPa·s at 25°C.

[0043] The above-mentioned shampoo preparation method adopts a phase-separated temperature-controlled feeding process: Phase A and Phase B are dissolved and emulsified at 80℃; the temperature is lowered to below 60℃ to add glycolipids, polyquaternium salts, and piroctone olamine salts; the temperature is further lowered to below 55℃ to add the heat-sensitive components of Poria cocos peel extract and fragrance. This process effectively avoids the destruction of triterpenoid active ingredients in Poria cocos peel extract by high temperature, thus preserving its antibacterial activity to the maximum extent. At the same time, high-speed dispersion at 1000-2000 r / min is used in step S1 to ensure that polymers such as hydroxypropyl guar gum and polyquaternium-10 are fully hydrated and free of lumps, improving the uniformity of the product's appearance and user experience.

[0044] The implementation schemes of the present invention will now be described in detail with reference to specific embodiments.

[0045] Experimental Example: Effects of Single Factors on Poria Cocos Peel Extract Test for pamoic acid: Chromatographic conditions: Column: Cosmosil C18 (250 mm × 4.6 mm, 5 μm), mobile phase: methanol-0.1% phosphoric acid aqueous solution, flow rate: 1.0 ml·min-1, detection wavelength: 210 nm, column temperature: 35℃.

[0046] Preparation of the standard curve for pachymic acid: 3 mg of pachymic acid was diluted to 20 ml with methanol, and then serially diluted to obtain a series of pachymic acid reference solutions with concentrations of 0.15 mg / ml, 0.075 mg / ml, 0.0375 mg / ml, 0.01875 mg / ml, and 0.009375 mg / ml. 20 µL of each solution was injected according to the chromatographic conditions described above, and the peak area of ​​each concentration was measured. Linear regression was performed with concentration as the abscissa and peak area as the ordinate, yielding the regression equation: Y = 1558116.42 + 7985.08 (R² = 0.9998). This indicates that pachymic acid exhibits good linearity within the concentration range of 0.009375–0.15 mg / ml. Figure 1 As shown.

[0047] a. A method for preparing a Poria cocos peel extract includes the following steps: 25g of Poria cocos peel was pretreated to obtain pretreated Poria cocos peel coarse powder. The coarse powder of Poria cocos peel was added to 200 mL of ethanol solutions of different concentrations and soaked for 20 min. The mixture was then refluxed at 65 °C for 45 min, filtered, and 50 mL of the filtrate was taken. The concentration of pachymic acid was measured and the extraction rate was calculated. The results are shown in Table 1 below.

[0048] Table 1: Extraction rate of pachymic acid at different ethanol concentrations

[0049] As can be seen from the data analysis in Table 1, the preferred concentration of ethanol in the preparation method of Poria cocos peel extract of the present invention is 90%.

[0050] b. A method for preparing a Poria cocos peel extract includes the following steps: 25g of Poria cocos peel was pretreated to obtain pretreated Poria cocos peel coarse powder. The coarse powder of Poria cocos peel was added to 200 mL of 90% ethanol solution and soaked for different times. It was then refluxed at 65℃ for 45 min, filtered, and 50 mL of the filtrate was taken to detect the concentration of pachymic acid and calculate the extraction rate. The results are shown in Table 2 below.

[0051] Table 2: Extraction rate of pachymic acid at different soaking times

[0052] As can be seen from the data analysis in Table 2, the soaking time is preferably 20 min in the preparation method of Poria cocos peel extract of the present invention.

[0053] c. A method for preparing a Poria cocos peel extract includes the following steps: 25g of Poria cocos peel was pretreated to obtain pretreated Poria cocos peel coarse powder. The coarse powder of Poria cocos peel was added to 200 mL of 90% ethanol solution and soaked for 20 min. It was then refluxed at 65℃ for different times, filtered, and 50 mL of the filtrate was taken to measure the concentration of pachymic acid. The extraction rate was calculated, and the results are shown in Table 3 below.

[0054] Table 3: Extraction rate of pachymic acid at different reflux extraction times

[0055] As can be seen from the data analysis in Table 3, the preferred reflux extraction time in the preparation method of Poria cocos peel extract of the present invention is 45 min.

[0056] In addition, a comparative experiment was conducted on Poria cocos extract. The results showed that Poria cocos extract contained more Poria cocos polysaccharides and less Poria cocos acid, which was difficult to extract and separate. This invention uses Poria cocos peel for extraction, which not only makes it easy to obtain the target product (triterpenoids including Poria cocos acid), but also makes use of waste and maximizes resource utilization.

[0057] Example 1: Based on the above results regarding the influence of single factors on the effective components of Poria cocos peel extract, the present invention preferably provides the following method for preparing Poria cocos peel extract, comprising the following steps: The Poria cocos peel was pretreated to obtain coarse powder of pretreated Poria cocos peel that passed through an 80-mesh sieve. At a material-to-liquid ratio of 25g:200mL, coarse Poria cocos peel powder was added to a 90% ethanol solution and soaked for 20min. The solution was then refluxed at 65℃ for 45min, filtered, and the filtrate was collected. The ethanol was recovered under reduced pressure and concentrated to obtain Poria cocos peel extract. Add water to the Poria cocos peel extract in an amount equivalent to 1 times that of the Poria cocos peel extract, stir to form a uniformly dispersed suspension to form an aqueous phase, first extract with 1 / 2 volume of petroleum ether 3 times, retain the aqueous phase, then extract with 1 / 2 volume of dichloromethane 1 to 3 times, retain the aqueous phase, then extract with 1 / 2 volume of ethyl acetate 1 to 3 times, take the ethyl acetate layer, recover the ethyl acetate under reduced pressure, and dry to obtain the Poria cocos peel extract.

[0058] Performance testing: The Poria cocos peel extract prepared in Example 1 was subjected to an antibacterial test against Malassezia furfur. The results showed that the Poria cocos peel extract inhibited Malassezia furfur, and the minimum inhibitory concentration was 12.5 μg / mL.

[0059] Example 2: The shampoo in Example 2 includes the raw materials shown in Table 4. Table 4: Raw materials for preparing the shampoo in Example 2

[0060] A method for preparing a shampoo containing Poria cocos peel extract, the method comprising the following steps: S1. Hydroxypropyl guar gum, hydroxypropyl trimethylammonium chloride, and polyquaternium salt-10 were added to water and dispersed evenly at a high speed of 1000 r / min. Then, tetrasodium diacetate of glutamic acid, sodium benzoate, and citric acid were added. The mixture was heated and stirred at 500 r / min at 75°C for 35 min to dissolve the mixture, thus obtaining phase A. S2. Cocamidopropyl betaine, sodium lauryl ether sulfate, ammonium lauryl sulfate, sodium cocoyl methyl taurate taurate, polyglycerol-10 lauryl ester, and stearamide dimethylamine lactate were added to water and heated and stirred at 300 r / min at 85°C for 35 min to dissolve, and kept at this temperature for 20 min to obtain phase B. S3. Mix phase A and phase B evenly, cool to 60°C, add glycolipid, polyquaternium-22, polyquaternium-52, hydrolyzed corn starch, and piroctone ethanolamine salt, and stir at 300 r / min for 25 min to obtain mixture A; S4. After the mixture A is cooled to 45°C, zinc lactate, decyl glucoside, PEG-40 hydrogenated castor oil, Poria cocos peel extract, 1,3-butanediol and amino-terminated polydimethylsiloxane are added to the mixture A. The mixture is stirred at 300 r / min for 20 min. Then, phenoxyethanol and fragrance are added and stirred evenly to obtain mixture B. S5. Add cocamide methyl MEA and sodium chloride to the mixture B, and stir at 300 r / min for 15 min to obtain shampoo.

[0061] Example 3: The shampoo of Example 3 comprises the raw materials shown in Table 5, and is otherwise the same as that of Example 2.

[0062] Table 5: Raw materials for preparing the shampoo in Example 3

[0063] Example 4: The shampoo of Example 4 comprises the raw materials shown in Table 6, and is otherwise the same as that of Example 2.

[0064] Table 6: Raw materials for preparing the shampoo in Example 4

[0065] Example 5: The shampoo of Example 5 comprises the raw materials shown in Table 7, and is otherwise the same as that of Example 2.

[0066] Table 7: Raw materials for preparing the shampoo in Example 5

[0067] Comparative Example 1: The difference between Comparative Example 1 and Example 5 is that no Poria cocos peel extract was added in Comparative Example 1, but otherwise it is the same as Example 5.

[0068] Comparative Example 2: The difference between Comparative Example 2 and Example 5 is that no pyrrolidone ethanolamine salt was added in Comparative Example 2, but otherwise it was the same as Example 5.

[0069] Comparative Example 3: The difference between Comparative Example 3 and Example 5 is that zinc lactate was not added in Comparative Example 3, but otherwise the same as in Example 5.

[0070] Comparative Example 4: The difference between Comparative Example 4 and Example 5 is that the preparation method in Comparative Example 4 is different, while the rest is the same as in Example 5; The preparation method of the shampoo in Comparative Example 4 is as follows: the raw materials for the preparation of shampoo in Table 7 are added one by one to the mixing tank, and the mixture is stirred at 75°C for 60 minutes to obtain the shampoo.

[0071] Comparative Example 5: The difference between Comparative Example 5 and Example 5 is that ketoconazole was used instead of Poria cocos peel extract in Comparative Example 5, while the rest is the same as in Example 5.

[0072] The shampoo samples prepared in Examples 2-5 and the shampoo samples prepared in Comparative Examples 1-5 were subjected to performance tests. The test methods and test results are as follows.

[0073] 1. Irritation Test: The Heteroalanine Transformation Test (HET-CAM) was used. Each shampoo sample (10% aqueous solution) was directly applied to the chorioalanine membrane of the chicken embryo. Bleeding, coagulation, and angiolysis were observed within 30 seconds. Irritation scores (IS, 0–20 points) were calculated: 0–2 for no irritation, 2–5 for mild irritation, 5–9 for moderate irritation, and ≥9 for strong irritation. The results are shown in Table 8.

[0074] 2. Stability test: Each shampoo sample was placed in a 40℃ constant temperature incubator and a -10℃ refrigerator for 3 months to observe whether there was any layering, precipitation, discoloration, etc. At the same time, the centrifugal stability (3000 r / min, 30 min) was measured and the layering was recorded. The test results are shown in Table 9.

[0075] 3. Antimicrobial activity against Malassezia furfur: Referring to the "Antimicrobial Test" method in the "Cosmetic Safety Technical Specifications," the diameter of the inhibition zone against Malassezia furfur in each shampoo sample was determined using the agar diffusion method (drilling method). The sample concentration was 10% (w / v, diluted with sterile water), with 100 μL added to each well. The samples were incubated at 30℃ for 48 h, and the diameter of the inhibition zone (mm) was measured. The test results are shown in Table 10.

[0076] 4. Clinical evaluation of dandruff-removing efficacy: Sixty volunteers (18-45 years old) with moderate dandruff were randomly divided into 10 groups (6 people in each group). They used the shampoos from Examples 2-5 and Comparative Examples 1-5, respectively, three times a week for four consecutive weeks. Dandruff severity was assessed using a scale of 0-4 (0=no dandruff, 1=mild, 2=moderate, 3=severe, 4=extremely severe), and the rate of decrease in score before and after use was calculated. Dandruff-removing efficacy = (score before use - score after use) / score before use × 100%. The test results are shown in Table 11.

[0077] Table 8: Stimulation Performance Test Results

[0078] The test results in Table 8 show that the shampoo formula of the present invention has a mild overall composition, while the chemical antidandruff agent ketoconazole used in Comparative Example 5 has a significant irritant effect on the chorioallantoic membrane of chicken embryos, and the natural Poria cocos peel extract is safer.

[0079] Table 9: Stability Test Results

[0080] As can be seen from the stability test in Table 9, the shampoo obtained by the present invention through optimization of ingredients and process has excellent overall stability, while the overall stability of the formulation system in Comparative Example 4 is not as good as that in Example 5.

[0081] Table 10: Results of Anti-Malassezia furfur Activity Test

[0082] Analysis of the data in Table 10 shows that the antibacterial activity increases with the increase of the content of Poria cocos peel extract. The activity of Comparative Examples 1-3 decreased significantly after the absence of any synergistic component, demonstrating that Poria cocos peel extract, piroctone olamine salt, and zinc lactate have a synergistic effect. Comparative Example 4 showed reduced efficacy due to the destruction of some active ingredients by high temperature. Although Comparative Example 5 (ketoconazole) exhibited the strongest activity, it was irritating (see Table 8), and long-term use could easily lead to drug resistance.

[0083] Table 11: Clinical evaluation results of dandruff-reducing efficacy (4 weeks)

[0084] Analysis of the data in Table 11 shows that the addition of Poria cocos peel extract in this invention achieves excellent dandruff removal effect. The lack of active ingredients in Comparative Examples 1-3 resulted in a less effective dandruff removal effect compared to Example 5, indicating that the ingredients in this formulation have a synergistic effect, enhancing the dandruff removal effect of the shampoo. The one-step high-temperature preparation method in Comparative Example 4 destroys some active substances, resulting in a less effective shampoo compared to Example 5. In Comparative Example 5, ketoconazole was used to replace the Poria cocos peel extract, achieving a dandruff removal effect of 96.7%, comparable to 95.8% in Example 5. This demonstrates that while achieving the same dandruff removal effect as chemical dandruff removers, this invention also possesses significant safety advantages (see Table 8), and the naturally derived Poria cocos peel extract is less prone to drug resistance, making it more suitable for long-term use.

[0085] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0086] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. A shampoo containing Poria cocos peel extract, characterized in that, The shampoo includes a poria cocos peel extract, which contains triterpenoids, including pachymic acid; and the amount of poria cocos peel extract added to the shampoo is 0.05% to 5% by mass percentage.

2. The shampoo according to claim 1, characterized in that, The preparation method of the Poria cocos peel extract includes the following steps: Poria peel is pretreated to obtain pretreated coarse powder of Poria peel; Add coarse powder of Poria cocos peel to an ethanol solution with a mass percentage concentration of 60-90%, soak for 10-30 minutes, reflux extract for 30-60 minutes, filter, collect the filtrate, recover the ethanol under reduced pressure, concentrate, and obtain Poria cocos peel extract. The Poria cocos peel extract is added to water in a volume equivalent to 1 to 3 times that of the Poria cocos peel extract, and stirred to form a uniformly dispersed suspension to form an aqueous phase. The aqueous phase is first extracted with petroleum ether 1 to 3 times, and the aqueous phase is retained. Then it is extracted with dichloromethane 1 to 3 times, and the aqueous phase is retained. Then it is extracted with ethyl acetate 1 to 3 times. The ethyl acetate layer is collected, and the ethyl acetate is recovered under reduced pressure. After drying, the Poria cocos peel extract is obtained.

3. The shampoo according to claim 2, characterized in that, The coarse powder of Poria cocos peel is passed through a 20-60 mesh sieve; The ratio of the coarse powder of Poria cocos peel to the ethanol solution is 1g:6~10mL; The volume ratio of Poria cocos peel extract to petroleum ether is 1:(0.5~2.5); the volume ratio of Poria cocos peel extract to dichloromethane is 1:(0.5~2.5); the volume ratio of Poria cocos peel extract to ethyl acetate is 1:(0.5~2.5).

4. The shampoo according to claim 1, characterized in that, The shampoo also includes the following ingredients by weight percentage: synergist A 0.1%~0.5%, synergist B 0.3%~2%, surfactant 15%~30%, conditioning agent 0.5%~2.0%, moisturizer 1%~3%, fragrance 0.1%~1.0%, preservative 0.1%~1.0%, pH adjuster 0.1%~0.3%, chelating agent 0.1%~0.5%, thickener 0.1%~3%, and the balance being water.

5. The shampoo according to claim 4, characterized in that, Synergist A is pyrrolidone ethanolamine salt; synergist B is zinc lactate.

6. The shampoo according to claim 5, characterized in that, The surfactant is at least one of cocamidopropyl betaine, sodium lauryl ether sulfate, ammonium lauryl sulfate, sodium cocoyl methyl taurate taurate, polyglycerol-10 lauryl ester, decyl glucoside, and lauryl ether-16.

7. The shampoo according to claim 6, characterized in that, The conditioning agent is at least one of hydroxypropyl guar hydroxypropyltrimethylammonium chloride, polyquaternium salt-10, polyquaternium salt-22, polyquaternium salt-52, and amino-terminated polydimethylsiloxane.

8. The shampoo according to claim 7, characterized in that, The humectant is 1,3-butanediol; the preservative is at least one of sodium benzoate, p-hydroxyacetophenone, and phenoxyethanol; the pH adjuster is citric acid; and the chelating agent is tetrasodium diacetate of glutamate.

9. The shampoo according to claim 8, characterized in that, The thickener is at least one of sodium chloride, cocamidomethyl MEA, hydrolyzed corn starch, hydroxypropyl cellulose, and hydroxypropyl methylcellulose.

10. A method for preparing a shampoo containing Poria cocos peel extract, characterized in that, The preparation method is used to prepare the shampoo containing Poria cocos peel extract as described in claim 9, and the preparation method includes the following steps: S1. Hydroxypropyl guar gum, hydroxypropyl trimethylammonium chloride, and polyquaternium salt-10 were added to water and stirred until evenly dispersed. Then, tetrasodium diacetate of glutamate, sodium benzoate, and citric acid were added, and the mixture was heated and stirred until dissolved to obtain phase A. S2. Cocamidopropyl betaine, sodium lauryl ether sulfate, ammonium lauryl sulfate, sodium cocoyl methyl taurate taurate, polyglycerol-10 lauryl ester, and stearamide-propyl dimethylamine lactate are added to water, heated and stirred to dissolve, and kept warm to obtain phase B. S3. Mix phase A and phase B evenly, cool down, add glycolipid, polyquaternium-22, polyquaternium-52, hydrolyzed corn starch, and piroctone ethanolamine salt, stir and treat to obtain mixture A; S4. After the mixture A is cooled to below 55°C, zinc lactate, decyl glucoside, PEG-40 hydrogenated castor oil, Poria cocos peel extract, 1,3-butanediol and amino-terminated polydimethylsiloxane are added to the mixture A, and the mixture is stirred. Then, phenoxyethanol and fragrance are added and stirred evenly to obtain mixture B. S5. Add cocamide methyl MEA and sodium chloride to the mixture B, and stir to obtain shampoo.

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