A hair breakage prevention and repair composition and its application in hair care products

Abstract

This application belongs to the field of daily chemical products and discloses an anti-breakage repair composition and its application in shampoo and conditioner. The technical solution is as follows: an anti-breakage repair composition, composed of *Elm chinensis* bark extract and *Edelweiss* extract, wherein the mass ratio of *Elm chinensis* bark extract to *Edelweiss* extract is 0.1-3:1-5. *Elm chinensis* bark extract is rich in polysaccharides and polyphenols. Its polysaccharides can form a protective film on the hair shaft, and its polyphenols can repair the scalp barrier. *Edelweiss* extract, on the one hand, is rich in sterols and long-chain fatty acids, which can interact with the lipids of the scalp stratum corneum to further repair the physical barrier and prolong the hair growth cycle. More importantly, both have good antioxidant capabilities, which can further nourish the scalp, improve the quality of new hair growth, reduce oxidative damage to the hair shaft, and enhance the hair's strength. In addition, the composition also contains *Asparagus cochinchinensis* root extract and *Eclipta prostrata* extract.

Description

Technical Field

[0001] This application relates to the field of daily chemical product manufacturing technology, and in particular to an anti-breakage repair composition and its application in hair care products. Background Technology

[0002] In recent years, hair loss, dry and brittle hair, and premature graying have become increasingly common, seriously affecting consumers' image and quality of life. Existing hair care products mostly focus on repairing the hair surface and improving its appearance, such as replenishing lipids, filling gaps in the hair cuticle, and providing temporary shine, but they pay relatively little attention to the fundamental factor of the scalp environment. Research shows that oxidative stress and chronic inflammation are important mechanistic factors leading to damaged hair health and hair loss. The scalp, as the microenvironment for hair follicles, generates reactive oxygen species (ROS) when affected by factors such as ultraviolet radiation, environmental pollution, irritation from unsuitable hair care products, and stress, leading to oxidative stress. Oxidative stress directly damages lipids, proteins, and DNA within hair follicle cells, ultimately causing follicle atrophy, prolonged resting phase, and premature entry into the regression phase, resulting in persistent hair loss. Furthermore, oxidative stress directly attacks keratin in the hair shaft and disrupts disulfide bonds crucial for maintaining the higher structure and function of hair, ultimately leading to increased hair permeability, decreased moisture content, reduced tensile strength, and loss of color and shine.

[0003] In this regard, cosmetic cleansing compositions containing a milky white extract, as disclosed in KR0163118B1, describe a cosmetic detergent composition containing an extract obtained from elm (Yubaekpi, Yukeunpi). Upon use, this extract increases blood flow, has antibacterial and astringent effects, thereby improving blood flow to the skin's capillaries and increasing skin cleansing power. The nutritional supply is effective in relieving symptoms of fungal infections, dandruff, etc., and has antiseptic and antibacterial properties. It can also make the skin more elastic and radiant, and has an astringent effect.

[0004] Chinese patent application 202411676095.9 discloses a plant composition for regulating the scalp microecological balance and its application. The composition describes extracts of Edelweiss and birch bark as active ingredients, which effectively inhibit Staphylococcus aureus and Malassezia restriction without affecting the normal proliferation of Staphylococcus epidermidis. Therefore, it can be used to regulate the scalp microecological balance, target and regulate the scalp microbiota, thereby reducing scalp sebum secretion and relieving scalp problems such as dandruff, scalp itching, scalp papules, sensitivity and redness, and excessive sebum secretion.

[0005] In summary, existing technologies have conducted some research on the scalp protection effects of extracts from elm and edelweiss, but no existing technologies have attempted to combine the two to explore the scalp protection capabilities of the mixture.

[0006] The problem this solution aims to solve is: how to provide a composition with the ability to repair the scalp and hair based on antioxidant properties. Summary of the Invention

[0007] The purpose of this application is to provide a composition that can enhance the scalp barrier function and strengthen hair follicle cell vitality, thereby improving the condition of the scalp itself and thus enhancing the strength of newly grown hair. In addition, it can also repair and reduce oxidative damage to hair through antioxidant effects, thereby enhancing the strength of hair.

[0008] To achieve the above objectives, this application discloses a hair breakage prevention and repair composition, which is composed of elm bark extract and edelweiss extract, wherein the mass ratio of elm bark extract to edelweiss extract is 0.1-3:1-5.

[0009] In the above composition, the bark extract of Elm tree is rich in polysaccharides and polyphenols. The polysaccharides can form a protective film on the hair strands, reducing friction and breakage. The polyphenols have anti-inflammatory effects and can repair the lipid barrier of the scalp. In addition, the phenolic compounds it contains can scavenge free radicals through a hydrogen donation mechanism. Furthermore, due to the protective layer formed on the hair strands and the antioxidant effect of the phenols, it can protect the hair strands from oxidative damage on the one hand, and inhibit the occurrence of oxidation on the other hand.

[0010] Edelweiss extract is rich in polysaccharides, sterols, and long-chain fatty acids, which can interact with the lipids of the scalp stratum corneum and repair the physical barrier by supplementing ceramide analogs. At the same time, its active ingredients can also reduce burning and stinging sensations caused by external stimuli to some extent, and inhibit histamine release, relieving sensitivity and discomfort.

[0011] More importantly, its rich polyphenolic compounds can chelate iron ions and other pro-oxidative metal ions, reducing the hydroxyl radicals generated by the Fenton reaction and thus inhibiting oxidative damage to hair.

[0012] Preferably, at least one of asparagus root extract and eclipta prostrata extract is also added.

[0013] Asparagus extract is rich in various steroidal saponins, polysaccharides, amino acids, and natural moisturizing factors. By supplementing natural moisturizing factors (NMF) and promoting the degradation of filaggrin, it powerfully repairs the scalp barrier. Its saponin components can regulate Langerhans cell activity and reduce immune inflammation. In addition, the polysaccharides in asparagus can scavenge superoxide anion free radicals, thereby inhibiting oxidative damage to hair and improving hair strength.

[0014] The extract of *Eclipta prostrata* is rich in terpenoids, alkaloids, polypeptides, flavonoids and polyphenols, which can directly penetrate into the hair follicles, promote the proliferation of hair papilla cells, prolong the hair growth phase, and thus reduce hair loss caused by premature transition of the resting phase. In addition, phenolic active substances can chelate pro-oxidative metal ions, reduce ROS generation, thereby inhibiting oxidative damage to hair and improving the strength of hair.

[0015] Preferably, the mixture contains asparagus root extract and eclipta prostrata extract, and the mass ratio of elm bark extract, edelweiss extract, asparagus root extract and eclipta prostrata extract is 0.1-3:1-5:1-10:1-10.

[0016] Preferably, the preparation method of the *Elm chinensis* bark extract is as follows: pulverize *Elm chinensis* bark into powder, add pure water at a solid-liquid ratio of 1:5-15, stir and extract at 55-65°C for 1-3 hours, filter and centrifuge to obtain *Elm chinensis* bark extract, concentrate the *Elm chinensis* bark extract to extract paste, and obtain *Elm chinensis* bark extract.

[0017] Preferably, the preparation method of the alpine edelweiss extract is as follows: pulverize alpine edelweiss into powder, add ethanol solution with a mass fraction of 45-60% at a solid-liquid ratio of 1:8-18, reflux extract at 60±2℃ for 1-3 hours, filter and centrifuge to obtain alpine edelweiss extract, concentrate alpine edelweiss extract to alcohol-free extract to obtain alpine edelweiss extract.

[0018] Preferably, the preparation method of the asparagus root extract is as follows: pulverize the asparagus root into powder, add pure water at a solid-liquid ratio of 1:10-20, stir and extract at a temperature of 55-70°C for 1-3 hours, filter and centrifuge to obtain the asparagus root extract, concentrate the asparagus root extract to an extract paste, and obtain the asparagus root extract.

[0019] Preferably, the preparation method of the snake intestine extract is as follows: snake intestine is crushed into powder, and an ethanol solution with a mass fraction of 45-65% is added at a solid-liquid ratio of 1:8-20. The mixture is stirred and extracted at a temperature of 45-65°C for 1-3 hours. The extract is obtained by filtration and centrifugation. The extract is then concentrated to an alcohol-free extract to obtain the snake intestine extract.

[0020] Preferably, the concentration process is specifically carried out by rotary evaporation at a temperature of 45-55℃, a pressure of -0.08 to -0.09 MPa, and a rotation speed of 45-60 r / min.

[0021] Furthermore, the preparation method of the anti-breakage repair composition is as follows: butylene glycol, glycerin and pure water are added to the elm bark extract, edelweiss extract, asparagus root extract and eclipta prostrata extract and mixed to obtain the anti-breakage repair composition.

[0022] In addition, this application also discloses the use of the above-described anti-breakage repair composition in the preparation of shampoo and conditioner products.

[0023] In addition, this application also discloses a hair care product containing 0.1 to 15 wt% of the anti-breakage and repair composition as described above.

[0024] The beneficial effects of this application are:

[0025] This application provides a composition that can enhance the scalp barrier function and strengthen hair follicle cell vitality, thereby improving the condition of the scalp itself and thus enhancing the strength of newly grown hair.

[0026] Furthermore, the elm bark extract in the composition is rich in polysaccharides and polyphenols. The polysaccharides can form a protective film on the hair strands, reducing friction and breakage; the polyphenols have anti-inflammatory effects and can repair the scalp lipid barrier. In addition, the abundant phenolic compounds in the composition can scavenge free radicals through a hydrogen donation mechanism.

[0027] Edelweiss extract is rich in polysaccharides, sterols, and long-chain fatty acids, which can interact with the lipids of the scalp stratum corneum and repair the physical barrier by supplementing ceramide analogs. At the same time, its active ingredients can also reduce burning and stinging sensations caused by external stimuli to some extent, and inhibit histamine release, relieving sensitivity and discomfort.

[0028] Its rich polyphenolic compounds can chelate iron ions and other pro-oxidative metal ions, reducing the hydroxyl radicals generated by the Fenton reaction.

[0029] More importantly, when the two plant extracts are used together, the composition produces a certain synergistic effect in terms of anti-oxidation. On the one hand, the two can improve the scalp microenvironment and enhance the quality of new hair through their antioxidant effects. On the other hand, the synergistic antioxidant ability of the two can also reduce oxidative damage to the hair and enhance the hair's toughness, thereby further enhancing the composition's repair ability. Attached Figure Description

[0030] Figure 1 Scanning electron microscope images of hair before and after using the hair conditioner in Example 12;

[0031] Figure 2 Scanning electron micrographs of hair before and after using the blank control group conditioner;

[0032] Figure 3 This is an observation image of chicken embryos before using the mixed extract;

[0033] Figure 4 This is an observation image of chicken embryos after using the mixed extract. Detailed Implementation

[0034] The present application will be clearly and completely described below with reference to its embodiments. It should be noted that, unless specific conditions are specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all commercially available conventional products.

[0035] Before presenting the embodiments and comparative examples, the source information of the plant extracts used in the embodiments and comparative examples of this application is first explained as follows:

[0036] The bark of the domesticated elm tree, commercially known as elm bark, was purchased from: Bozhou Tianyinge Pharmaceutical Co., Ltd.

[0037] Edelweiss from the Alpine region, commercially known as Edelweiss, purchased from: Qizhou Medicinal Herbs Hall;

[0038] Asparagus root, commercially known as Asparagus, was purchased from: Bozhou Tianyinge Pharmaceutical Co., Ltd.;

[0039] The product, Eclipta prostrata, was purchased from Bozhou Tianyinge Pharmaceutical Co., Ltd., under the trade name Mohanlian.

[0040] Furthermore, it should be noted that the preparation methods for each plant extract in this application are as follows:

[0041] The preparation method of the elm bark extract is as follows: elm bark is crushed into powder, pure water is added at a solid-liquid ratio of 1:10±2, and the mixture is stirred and extracted at 60±5℃ for 2 hours. The elm bark extract is obtained by filtration and centrifugation. The elm bark extract is concentrated to an extract by rotary evaporation at a temperature of 50±5℃, a pressure of -0.09MPa, and a rotation speed of 50±5r / min.

[0042] The preparation method of Edelweiss extract is as follows: Edelweiss is pulverized into powder, and ethanol solution with a mass fraction of 55±5% is added at a solid-liquid ratio of 1:12±2. The mixture is refluxed at 60±2℃ for 2 hours, filtered and centrifuged to obtain Edelweiss extract. The Edelweiss extract is concentrated to extract by rotary evaporation at a temperature of 50±5℃, a pressure of -0.09MPa, and a rotation speed of 50±5r / min to obtain Edelweiss extract.

[0043] The preparation method of Asparagus root extract is as follows: Asparagus root is crushed into powder, pure water is added at a solid-liquid ratio of 1:15±2, and the mixture is stirred and extracted at 65±5℃ for 2 hours. The extract is obtained by filtration and centrifugation. The extract is then concentrated to a paste by rotary evaporation at a temperature of 50±5℃, a pressure of -0.09MPa, and a rotation speed of 50±5r / min to obtain Asparagus root extract.

[0044] The preparation method of snake intestine extract is as follows: snake intestine is crushed into powder, and ethanol solution with a mass fraction of 55±5% is added at a solid-liquid ratio of 1:15±2. The mixture is stirred and extracted at 55±5℃ for 2 hours. After filtration and centrifugation, snake intestine extract is obtained. The snake intestine extract is concentrated by rotary evaporation at a temperature of 50±5℃, a pressure of -0.09MPa, and a rotation speed of 50±5r / min to obtain an alcohol-free extract.

[0045] Preparation method of the anti-breakage repair composition: The plant extracts are added to a mixture of butylene glycol, glycerin and purified water to obtain the anti-breakage repair composition. The content of butylene glycol in the anti-breakage repair composition is 10.2 wt%, the content of glycerin is 20.3 wt%, and the content of purified water is 59.4 wt%.

[0046] In addition, for specific details regarding the selection of plant extracts and the choice of mass ratios between plant extracts, please refer to the specific explanations in each case study.

[0047] Examples 1-12

[0048] A hair breakage prevention and repair composition, the formula of which is shown in Table 1 (it should be noted that the values ​​in Table 1 specifically represent the mass ratio between the various plant extracts):

[0049] Table 1: Formulation Tables for Examples 1-12

[0050] Example 1 0.1 5 0 0 Example 2 0.2 3.5 0 0 Example 3 0.5 2.5 0 0 Example 4 1.5 1.5 0 0 Example 5 3 1 0 0 Example 6 0.5 2.5 1 0 Example 7 0.5 2.5 4 0 Example 8 0.5 2.5 10 0 Example 9 0.5 2.5 0 1 Example 10 0.5 2.5 0 3 Example 11 0.5 2.5 0 10 Example 12 0.5 2.5 4 3

[0051] Comparative Examples 1-3

[0052] A composition, the formulation of which is shown in Table 2 (it should be noted that the values ​​in Table 2 specifically represent the mass ratio between the various plant extracts):

[0053] Table 2: Formulation Tables for Comparative Examples 1-3

[0054] Comparative Example 1 0.5 0 0 0 Comparative Example 2 0 2.5 0 0 Comparative Example 3 0 0 4 3

[0055] Performance testing:

[0056] 1. DPPH free radical scavenging rate test

[0057] 1.1 Experimental Objectives and Principles

[0058] Excessive production of free radicals can lead to natural skin aging and photoaging, resulting in wrinkles. Therefore, the ability to scavenge free radicals is one of the important indicators for evaluating anti-aging and antioxidant cosmetics (raw materials).

[0059] For hair, oxidative damage can disrupt the integrity of its structure. The DPPH free radical scavenging rate can evaluate whether hair care ingredients can effectively prevent oxidative damage, thereby indirectly evaluating their anti-breakage efficacy.

[0060] 1.2 Test Indicators

[0061] Criteria for determining DPPH free radical scavenging rate: If the DPPH scavenging rate of the sample is higher than that of the negative control and the difference is statistically significant (P<0.05), then the test sample can be considered to have antioxidant effect.

[0062] 1.3 Test Materials

[0063] 1.3.1 Instruments and Equipment

[0064] BSA224S Analytical Balance

[0065] L6s UV spectrophotometer

[0066] 1.3.2 Reagents

[0067] DPPH (1,1-diphenyl-2-picrylhydrazine), 98%

[0068] 1.3.3 Experimental group treatment

[0069] Sample: The anti-breakage repair compositions prepared in Examples 1-12 and Comparative Examples 1-3 were diluted to a concentration of 5% using pure water.

[0070] Positive control (vitamin E, purity ≥96%): Vitamin E was diluted to 0.1% by mass with a 95% ethanol solution.

[0071] Negative control: pure water.

[0072] 1.4 Experimental Procedure:

[0073] Set up sample tubes, sample background tubes, DPPH tubes, and solvent background tubes. Each group should have 3 parallel tubes. Add different reagent solutions to each of the four groups, shake gently, and let stand at room temperature for 5 minutes. Transfer the reaction solutions of each group into a 1 cm cuvette and measure the absorbance at 517 nm.

[0074] 1.5 Result Calculation

[0075] Calculate the DPPH radical scavenging rate according to Equation 1.

[0076] Formula 1

[0077] In the formula:

[0078] T—is the absorbance of the sample tube, that is, the absorbance of the solution after the sample reacts with DPPH;

[0079] T0—is the background absorbance of the sample;

[0080] C—is the absorbance of the DPPH tube, i.e., the absorbance of the DPPH solution without the sample added;

[0081] C0 — represents the background absorbance of the solution.

[0082] 1.6 Data Analysis

[0083] The independent samples t-test was used to compare the DPPH free radical scavenging rates among the test samples, positive control, and negative control. All statistical analyses were two-tailed tests with a significance level of α=0.05. P>0.05 indicated no significant difference between the two groups; P<0.05 indicated a significant difference between the two groups.

[0084] 1.7 Test results are shown in Table 3.

[0085] Table 3: DPPH Free Radical Scavenging Rate Test Results

[0086]

[0087] 1.7 Results Analysis

[0088] 1. As can be seen from the observation of Examples 1-5, when the mass ratio between the bark extract of Elm tree and the extract of Edelweiss was changed in Examples 1-5, the free radical scavenging ability of Examples 1-5 fluctuated to a certain extent, but the fluctuation range was relatively small overall.

[0089] 2. Further observation of Examples 3 and 6-11 shows that when Asparagus root extract or Eclipta prostrata extract is further added to the composition, the free radical scavenging ability of Examples 6-11 is improved compared with that of Example 3. This may be due to the strong free radical scavenging ability of Asparagus root extract or Eclipta prostrata extract itself.

[0090] On the other hand, it cannot be ruled out that the mixture of Asparagus root extract or Eclipta prostrata extract with Elm bark extract and Edelweiss extract may have a synergistic effect to enhance the free radical scavenging ability of the composition.

[0091] 3. Observations of Examples 7, 10, and 12 show that the free radical scavenging rate of Example 7 reached approximately 42.18%, and the free radical scavenging rate of Example 10 reached approximately 38.54%.

[0092] Therefore, theoretically, the expected value of Example 12 should be approximately 40.36% (free radical scavenging rate of Example 7 / 2 + free radical scavenging rate of Example 10 / 2).

[0093] However, observing that the actual value of Example 12 reached 46.10%, it can be seen that the actual value increased by about 5.8% of the expected value. Therefore, it can be seen from Examples 7, 10, and 12 that there is at least a synergistic effect between Asparagus root extract and Eclipta prostrata extract.

[0094] 4. As can be seen from Examples 1-5 and Comparative Examples 1-2, Comparative Example 1, when using only the bark extract of Elm chinensis, achieved a free radical scavenging rate of 27.61%;

[0095] Comparative Example 2, when only Edelweiss extract was used, achieved a free radical scavenging rate of approximately 25.76%;

[0096] Therefore, theoretically, the expected value of Example 3 should be the free radical scavenging rate of Comparative Example 1 × the mass percentage of Elm bark extract in the composition of Example 3 + the free radical scavenging rate of Comparative Example 2 × the mass percentage of Edelweiss extract in the composition of Example 3, that is, the expected value of Example 1 is approximately 25.80%;

[0097] Similarly, the expected value for Example 2 is 25.86%;

[0098] The expected value for Example 3 was 26.07%;

[0099] The expected value for Example 4 was 26.69%;

[0100] The expected value for Example 5 was 27.15%;

[0101] It is evident that the actual values ​​of Examples 1-5 are all higher than the expected values ​​of Examples 1-5. This indicates that within the mass ratio range recorded in this application, the combination of Elm bark extract and Edelweiss extract has a certain synergistic effect, and the synergistic effect of Examples 1-3 is particularly obvious.

[0102] Further observation of Examples 3, 12 and Comparative Example 3 shows that the expected value of Example 12 predicted using the above method should be between that of Example 3 and Comparative Example 3. However, the free radical scavenging ability of Example 12 is even higher than that of Example 3. This shows that the mixture of Elm bark extract and Edelweiss extract in this scheme also has a certain synergistic effect with the mixture of Asparagus root extract and Eclipta prostrata extract.

[0103] 2. Cell viability test

[0104] 2.1 Experimental Objectives and Principles

[0105] Cell viability assays are based on MTT (Osprey Blue), a reducing agent for live cell metabolites. MTT acts on the respiratory chain in the mitochondria of live cells, producing blue crystals under the action of succinate dehydrogenase and cytochrome. The amount of crystals produced is directly proportional to the number of live cells.

[0106] 2.2 Test Indicators

[0107] Criteria for determining cell activity: If the cell activity value of the sample group is higher than that of the negative group, it is considered that the sample can promote the proliferation of keratinocytes and the proliferation and metabolism of the stratum corneum at this concentration, and thus it is inferred that the sample has a certain repair effect.

[0108] 2.3 Instruments and Equipment

[0109] RT-6100 ELISA Analyzer

[0110] 2.4 Cells used in the experiment

[0111] keratinocytes

[0112] 2.5 Test Methods

[0113] (1) Sample processing

[0114] Sample group: The original sample solution was filtered through a 0.22 μm filter, and the filtrate was collected as the sample stock solution. Then, the sample stock solution was diluted with pure water to a mass fraction of 0.0625% in the solution.

[0115] Negative control: basal culture medium.

[0116] (2) Experimental operation procedures

[0117] Cells were seeded into 96-well plates, and after 24 hours, the culture medium was discarded. Basic culture medium containing different concentrations of the sample was added, and OD was measured using the MTT assay after 24 hours. 490nm The effects of the samples on cell viability were determined through data analysis.

[0118] 2.6 Calculation Formula

[0119] The calculation formula is shown in Equation 2:

[0120] Formula 2

[0121] 2.7 Test results are shown in Table 4.

[0122] Table 4: Results of Cell Viability Test

[0123]

[0124] 2.8 Results Analysis

[0125] Results Analysis

[0126] 1. As can be seen from the observation of Examples 1-5, when the mass ratio between the bark extract of Elm tree and the extract of Edelweiss was changed in Examples 1-5, the cell activity promoting ability of Examples 1-5 fluctuated to a certain extent, but the overall fluctuation range was relatively small.

[0127] 2. Further observation of Examples 3 and 6-11 shows that when Asparagus root extract or Eclipta prostrata extract is further added to the composition, the cell activity promoting ability of Examples 6-11 is improved compared with that of Example 3. This may be due to the strong cell activity promoting ability of Asparagus root extract or Eclipta prostrata extract itself.

[0128] On the other hand, it cannot be ruled out that the mixture of Asparagus root extract or Eclipta prostrata extract with Elm bark extract and Edelweiss extract may have a synergistic effect to enhance the cell activity promoting ability of the composition.

[0129] 3. Observations of Examples 7, 10, and 12 show that the cell viability of Example 7 reached approximately 113.34%, and the cell viability of Example 10 reached approximately 110.72%.

[0130] Therefore, theoretically, the expected value of Example 12 should be approximately 112.03% (cell viability of Example 7 / 2 + cell viability of Example 10 / 2).

[0131] However, observing that the actual value of Example 12 reached 115.70%, it can be seen that the actual value increased by about 3.7% of the expected value. Therefore, it can be seen from Examples 7, 10, and 12 that there is at least a synergistic effect between Asparagus root extract and Eclipta prostrata extract.

[0132] 4. As can be seen from Example 3 and Comparative Examples 1-2, in Comparative Example 1, the cell activity reached 103.75% when only the bark extract of *Ulmus pumila* was used;

[0133] Comparative Example 2, when only Edelweiss extract was applied, showed a cell activity of approximately 102.22%;

[0134] Therefore, theoretically, the expected value for Example 3 should be approximately 102.25%;

[0135] However, the actual value (106.61) of Example 3 was higher than its expected value, which shows that the combination of Elm bark extract and Edelweiss extract has a certain synergistic effect.

[0136] Further observation of Examples 3, 12 and Comparative Example 3 shows that the expected value of Example 12 predicted using the above method should be between that of Example 3 and Comparative Example 3. However, the cell activity promoting ability of Example 12 is even higher than that of Example 3. It can be seen that the mixture of Elm bark extract and Edelweiss extract in this scheme also has a certain synergistic effect with the mixture of Asparagus root extract and Eclipta prostrata extract.

[0137] 3. Hair strength test

[0138] 3.1 Experimental Objectives and Principles

[0139] The strength of hair strands is determined by their tensile properties; strands that are difficult to stretch or break are considered strong. Strength is typically expressed by parameters such as tensile force (load), stress, elongation, and work done throughout the process at the fracture point, yield point, or a specified point. This method involves uniformly stretching a single hair strand of equal length on a tensile testing instrument until the strand breaks, and then calculating the tensile strength and breaking work based on the curve. Since ambient temperature and humidity affect the tensile properties of hair, temperature and humidity control measures are required during the test to ensure stability of the test environment throughout the experiment.

[0140] This test method is an in vitro method, suitable for testing the hair breakage prevention efficacy of cosmetics.

[0141] 3.2 Test indicators, see Table 5.

[0142] Table 5: Test Indicators

[0143] tensile strength The higher the measured value, the stronger the hair strand; compared with the baseline test, the tensile strength of the sample treatment test increased significantly, indicating that the sample has the ability to improve the strength of the hair strand and has the effect of preventing hair breakage.

[0144] 3.3 Experimental Materials and Methods

[0145] 3.3.1 Instruments and Equipment

[0146] Combing tester: XJ810

[0147] Electronic balance: JJ1000

[0148] Digital micrometer: 0-25mm / 0.001mm

[0149] Mildly damaged hair strands: length ≥10cm, net weight ≥6g

[0150] 3.3.2 Reagents

[0151] Cleaning solution: K12 (sodium dodecyl sulfate)

[0152] 3.3.3 Test Environment

[0153] The test environment was 26°C ± 2°C and the relative humidity was 60% ± 10%.

[0154] 3.4 Test Methods

[0155] (1) Sample processing test

[0156] Apply 0.2g of sample per gram of hair strand evenly to the surface of the hair strand for approximately 20-30 seconds; then rinse and pat dry the surface of the hair strands. Place the real human hair strands in a constant temperature and humidity chamber and let them stand for 24 hours. After that, remove the hair strands and perform a single-strand tensile test.

[0157] The formulations of the above samples are shown in Table 6:

[0158] Table 6: Sample Formulation Table

[0159]

[0160] In Example 12, the hair conditioner's E phase contained the anti-breakage repair composition prepared in Example 12, while the blank control group's hair conditioner's E phase contained pure water.

[0161] The preparation method of the above samples:

[0162] Step 1: Disperse and dissolve phase D in advance until it is a transparent, particle-free liquid; Disperse and dissolve phase F in pure water above 85 degrees Celsius in advance until it is a transparent, particle-free liquid;

[0163] Step 2: Add the pure water from phase A to the homogenizing pot, and add hydroxyethyl cellulose under homogenization conditions, and homogenize and disperse evenly.

[0164] Step 3: Heat to 80-85℃ and add each raw material of phase B in sequence. Stir evenly, then vacuum homogenize for 5 minutes, keep warm for 30 minutes, vacuum homogenize for 5 minutes, and then cool down.

[0165] Step 4: Cool down to 60-65℃, add phase C and phase D in sequence, stir evenly, and then vacuum homogenize for 5 minutes.

[0166] Step 5: Cool to 45-50℃, add the E phase sequentially and stir until homogeneous, then vacuum homogenize for 3-5 minutes. Next, add the F phase raw material and stir slowly until homogeneous to obtain the finished product.

[0167] (2) Experimental operation procedures

[0168] Rinse the isolated hair sample with running, constant-temperature water or skip rinsing according to the sample usage instructions. Apply 0.2 grams of sample per gram of hair strand, evenly to the hair surface, then rinse and blot dry or skip rinsing. Place the isolated hair in a constant-temperature and humidity environment to air dry naturally for 24 hours, then perform a single-strand tensile test.

[0169] 3.5 Calculation Formula

[0170] The calculation formula is shown in Equation 3:

[0171] Formula 3

[0172] 3.5 Test results are shown in Table 7.

[0173] Table 7: Tensile Strength Improvement Rate

[0174] Example 12 Hair Conditioner 3.91 P=0.048 Effective Blank control group hair conditioner 1.37 P=0.416 No effect

[0175] 3.6 Results Analysis

[0176] As shown in Table 7, the tensile strength of hair strands treated with the hair conditioner of Example 12 increased by 3.91% compared to before treatment. There was a significant difference between the treated hair strands and the data from the two groups before treatment (P < 0.050), indicating that the sample has anti-breakage efficacy. However, for the hair strands using the blank control group hair conditioner, there was no significant difference between the treated hair strands and the data from the two groups before treatment (P > 0.050), indicating that the sample did not have anti-breakage efficacy. Table 6 shows that the only difference between the two groups of samples was the addition of the anti-breakage repair composition prepared in Example 12 or pure water to phase E. Therefore, it can be concluded that the anti-breakage repair composition prepared in Example 12 has anti-breakage efficacy.

[0177] 4. Scanning electron microscope

[0178] 4.1 Experimental Objectives and Principles

[0179] Hair consists of three parts: the cuticle, cortex, and medulla. The cuticle is the outermost structure of the hair, tightly wrapping the cortex like scales to protect hair fibers from mechanical and environmental damage. Due to daily factors such as perming, bleaching, sun exposure, combing, and blow-drying, the cuticles can be damaged, causing them to lift or fall off, resulting in dry, dull hair. This method involves treating damaged hair strands externally and observing the cuticles using scanning electron microscopy. By comparing the changes in the cuticles before and after treatment, the repair efficacy of the sample on the hair cuticle is evaluated.

[0180] 4.2 Experimental Materials and Methods

[0181] 4.2.1 Instruments and Equipment

[0182] Scanning electron microscope: KYKY-EM6200

[0183] Mildly damaged hair strands: length ≥10cm, net weight >6g

[0184] 4.2.2 Reagents

[0185] Cleaning solution: K12 (sodium dodecyl sulfate)

[0186] 4.2.3 Test Environment

[0187] The test environment temperature was 22°C ± 2°C, and the relative humidity was 50% ± 10%.

[0188] 4.3 Test Methods

[0189] Sample processing test

[0190] Apply 0.2 grams of sample evenly to the surface of each hair bundle for about 30 seconds, let stand for about 3-5 minutes, place in a constant temperature and humidity chamber for more than 4 hours until the hair is dry, remove the hair bundle and fix it in the test position, and start the scanning electron microscope test.

[0191] 4.5 Test Results

[0192] SEM images of mildly damaged in vitro hair before and after using the conditioner in Example 12 are shown below. Figure 1 As shown, the scanning electron microscope images of hair before and after using the blank control group conditioner are as follows. Figure 2 As shown. (Through) Figure 1 and Figure 2 It can be seen that before using the two groups of samples, the hair cuticles were raised, and some were peeled off unevenly. After using the two groups of samples, it can be seen that some hair cuticles were slightly closed, indicating that both groups of samples have a certain repairing effect on the hair cuticles. However, the hair cuticle closure after using the hair conditioner of Example 12 was better than that after using the blank control group hair conditioner, indicating that the repairing effect of the hair conditioner of Example 12 was better.

[0193] 5. Chicken embryo chorioallantoic membrane irritation test

[0194] 5.1 Instruments and Equipment

[0195] Fully automatic incubator, stereomicroscope, SPF chicken embryos.

[0196] 5.2 Reagents

[0197] Sodium chloride;

[0198] Sodium dodecyl sulfate.

[0199] 5.3 Test Methods

[0200] (1) Experimental group treatment and reagents

[0201] Sample group: The bark extract of Elm tree, the extract of Edelweiss, the root extract of Asparagus cochinchinensis and the extract of Eclipta prostrata were mixed in a weight ratio of 1:1:1:1. Then, the mixture of the above raw materials was added to butylene glycol, glycerin and pure water to obtain a mixed extract, wherein the content of butylene glycol was 10.2 wt%, the content of glycerin was 20.3 wt% and the content of pure water was 9.5 wt%.

[0202] Negative control group: 0.9% sodium chloride solution;

[0203] Positive control group: 1% sodium dodecyl sulfate solution.

[0204] (2) Experimental procedures

[0205] In this test, 6 chicken embryos were selected for each group. The condition of the chorioallantoic membrane was recorded using a photographic device. The sample to be tested was added to the chorioallantoic membrane of the chicken embryo, the time of sample addition was recorded, and the air cell was covered with a moistened plastic wrap. The chicken embryos were then transferred to a constant temperature and humidity incubator for culture. The degree of change of each toxic effect was observed and photographed.

[0206] (3) Observation of results

[0207] Observe and record bleeding, coagulation, and vascularization manifestations, and score them according to their severity.

[0208] (4) Data Analysis

[0209] The scoring criteria for bleeding, coagulation, and angiogenesis are shown in Table 8:

[0210] Table 8: Scoring Criteria

[0211]

[0212] The endpoint evaluation method was used for the experiment, and the endpoint evaluation (ES) was calculated. The results were retained to two decimal places. The score for each chicken embryo was the sum of the observed bleeding, coagulation and vascularization in each chicken embryo. The ES = 6 was the average of the mathematical sums obtained from the chicken embryos.

[0213] 5.4 Result Judgment Criteria:

[0214] ES≤4, non-irritating; 4<ES≤12, mildly irritating; 12<ES<16, moderately irritating; ES≥16, highly irritating / corrosive.

[0215] 5.5 Results Analysis

[0216] The following are observation images of chicken embryos before and after using the mixed extract: Figure 3-4 As shown, where Figure 3 This is an observation image of chicken embryos before using the mixed extract. Figure 4 The image shows the chicken embryos after using the mixed extract. The sample group had an ES value of 3.67, indicating that it was non-irritating.

[0217] Since the concentration of each extract in the mixed extract is 15wt%, there is no irritation when mixed at high concentrations, which further proves that the technical solution of the present invention is mild and non-irritating.

[0218] Application Example 1

[0219] A hair breakage prevention and repair product is prepared according to the formula in Table 9. The preparation method is as follows: the hair breakage prevention composition prepared in Example 12 is prepared by mixing all raw materials according to the formula in Table 9 and sterilizing at 80±3℃ for 1 hour.

[0220] Table 9: Ingredient List of Hair Breakage Prevention and Repair Products

[0221] The anti-hair breakage composition prepared in Example 12 98.5 1,2-Hexanediol 1 p-Hydroxyacetophenone 0.5

[0222] Application Example 2

[0223] A hair breakage prevention and repair shampoo, the formula of which is shown in Table 10:

[0224] Table 10: Formula Table for Anti-Hair Breakage and Repair Shampoo

[0225]

[0226] The preparation method of the above-mentioned anti-breakage and repair shampoo includes the following steps:

[0227] Step 1: First, disperse phase A1 in cold water by stirring until there are no particles (no white particles). While stirring, heat the mixture to 60°C, then add phase A2 and keep it warm for 10 minutes.

[0228] Step 2: Add the raw materials of phase B to the main beaker in sequence, stir to dissolve, and keep warm for 5 minutes;

[0229] Step 3: Cool down to 45℃, add phase C and phase D, adjust the pH to 5.0-5.5, and then add phase E;

[0230] Step 4: Add phase F and stir well to obtain anti-breakage repair shampoo.

Claims

1. A hair breakage prevention and repair composition, characterized in that, It includes elm bark extract and edelweiss extract, wherein the mass ratio of elm bark extract to edelweiss extract is 0.1-3:1-5.

2. The anti-breakage repair composition according to claim 1, characterized in that, It also contains at least one of the following: asparagus root extract and eclipta prostrata extract.

3. The anti-breakage and repair composition according to claim 1, characterized in that, It also contains a mixture of Asparagus root extract and Eclipta prostrata extract, and the mass ratio of Elm bark extract, Edelweiss extract, Asparagus root extract and Eclipta prostrata extract is 0.1-3:1-5:1-10:1-10.

4. The anti-breakage repair composition according to claim 1, characterized in that, The preparation method of the *Elm chinensis* bark extract is as follows: pulverize *Elm chinensis* bark into powder, add pure water at a solid-liquid ratio of 1:5-15, stir and extract at 55-65℃ for 1-3 hours, filter and centrifuge to obtain *Elm chinensis* bark extract, concentrate the *Elm chinensis* bark extract to extract paste, and obtain *Elm chinensis* bark extract.

5. The anti-breakage and repair composition according to claim 1, characterized in that, The preparation method of the alpine edelweiss extract is as follows: pulverize alpine edelweiss into powder, add ethanol solution with a mass fraction of 45-60% at a solid-liquid ratio of 1:8-18, reflux extract at 60±2℃ for 1-3 hours, filter and centrifuge to obtain alpine edelweiss extract, concentrate alpine edelweiss extract to alcohol-free extract to obtain alpine edelweiss extract.

6. The anti-breakage repair composition according to claim 2, characterized in that, The preparation method of the asparagus root extract is as follows: pulverize the asparagus root into powder, add pure water at a solid-liquid ratio of 1:10-20, stir and extract at a temperature of 55-70℃ for 1-3 hours, filter and centrifuge to obtain the asparagus root extract, concentrate the asparagus root extract to extract paste, and obtain the asparagus root extract.

7. The anti-breakage repair composition according to claim 2, characterized in that, The preparation method of the snake intestine extract is as follows: snake intestine is crushed into powder, and ethanol solution with a mass fraction of 45-65% is added at a solid-liquid ratio of 1:8-20. The mixture is stirred and extracted at a temperature of 45-65°C for 1-3 hours. The extract is obtained by filtration and centrifugation. The extract is then concentrated to an alcohol-free extract to obtain the snake intestine extract.

8. The anti-breakage repair composition according to any one of claims 4-7, characterized in that, The concentration process specifically involves rotary evaporation at a temperature of 45–55°C, a pressure of -0.08–0.09 MPa, and a rotation speed of 45–60 r / min.

9. Use of the anti-breakage repair composition according to any one of claims 1-8 in the preparation of shampoo and conditioner products.

10. A personal care product, characterized in that, Containing 0.1 to 15 wt% of the anti-breakage repair composition as described in any one of claims 1-8.

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