A skin repair composition and its preparation method

Abstract

This invention relates to a skin repair composition and its preparation method, belonging to the field of cosmetic technology. The composition comprises the following components in weight percentages: 5%–10% grapefruit extract, 0.1%–2% licorice root extract, 15%–50% yeast / barley seed fermentation product filtrate, and 40%–70% polyol, with the polyol content made up to 100%, and the naringin content in the composition is not less than 5000 ppm. The composition is prepared using polyol as the continuous phase through a specific stepwise feeding process, vacuum degassing, static aging, and filtration quality control. This composition achieves stable dispersion of high-content fermentation filtrate and plant extracts under anhydrous conditions, exhibits high naringin retention, possesses excellent antibacterial properties without the need for additional preservatives, and also demonstrates significant antioxidant and anti-inflammatory effects.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a skin repair composition and its preparation method. Background Technology

[0002] The skin is a vital barrier against external stimuli and prevents moisture loss. In daily life, the skin is highly susceptible to damage from both internal and external factors. External damage mainly includes ultraviolet radiation, environmental pollution, chemical irritation, mechanical trauma, and iatrogenic damage such as laser cosmetic procedures and microneedling treatments. Internal damage includes aging, metabolic disorders, oxidative stress, inflammatory responses, and genetic factors. These damaging factors can lead to impaired skin barrier function, increased moisture loss from the stratum corneum, release of inflammatory factors, collagen degradation, and damage to the extracellular matrix (ECM) structure, thereby causing problems such as dry skin, sensitivity, pigmentation, deepened wrinkles, and delayed wound healing.

[0003] Traditional skin repair methods mainly rely on the topical application of single active ingredients such as vitamin C, hyaluronic acid, and ceramides. While these ingredients can alleviate skin symptoms to some extent, they often suffer from low bioavailability, limited target sites, and limited repair effects, making them inadequate for addressing complex skin damage. Currently, plant extracts are often combined with microbial fermentation products to achieve multiple effects such as anti-oxidation, anti-inflammation, and barrier repair. For example, Chinese patent CN105055296A discloses a skincare composition containing yeast fermentation product filtrate, which explicitly includes yeast / barley seed fermentation product filtrate, but in low concentrations, and the composition uses water as the continuous phase. Chinese patent CN112137904A discloses an essence containing licorice root extract and yeast / rice fermentation product filtrate, also using water as the main solvent, and combined with various other plant extracts. However, these combinations have poor compatibility. If the proportion of fermentation filtrate is increased while the aqueous phase is reduced or removed, the system is prone to flocculation, precipitation, or stratification, making it difficult to guarantee physical stability. The active ingredients in plant extracts, such as flavonoids, exhibit poor stability in conventional aqueous systems, with their content decreasing significantly after long-term storage, making it difficult to maintain effective dosage levels. Achieving stable retention of active ingredients while simplifying the formulation system remains a challenge that current technologies have not yet solved.

[0004] Therefore, there is an urgent need to provide a composition that can both stabilize the active ingredients and repair the skin. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a skin repair composition and its preparation method. The composition uses a polyol as the continuous phase and is formulated by blending grapefruit extract, licorice root extract, and yeast / barley seed fermentation product filtrate in a specific ratio, while controlling the naringin content to be no less than 5000 ppm. This achieves excellent physical stability, chemical stability, and consistent efficacy under a high active ingredient load.

[0006] To achieve the above objectives, the present invention provides the following technical solution to address the technical problem: In a first aspect, the present invention provides a skin repair composition comprising, by weight percentage, 5% to 10% grapefruit extract, 0.1% to 2% licorice root extract, 15% to 50% yeast / barley seed fermentation product filtrate, and the balance being polyols. The composition contains not less than 5000 ppm of naringin.

[0007] In the composition, flavonoids such as naringin in grapefruit extract and active substances such as glycyrrhizin chalcone in licorice root extract have a synergistic effect in anti-inflammatory and antioxidant properties. The yeast / barley seed fermentation product filtrate is rich in organic acids, polysaccharides and small molecule active peptides. On the one hand, it can act as a natural solubilizer to help disperse highly polar plant extracts. On the other hand, its weakly acidic environment is conducive to the stability of naringin. As a continuous phase, polyol provides an anhydrous environment to avoid hydrolysis of active ingredients and promotes uniform dispersion of each component through its good compatibility and moisturizing properties.

[0008] Preferably, the polyol is butanediol.

[0009] Preferably, the pH value of the composition is 4.0 to 7.0 under direct measurement at 25°C.

[0010] Preferably, the composition has a relative density of 1.000 to 1.100 and a refractive index of 1.4050 to 1.4250.

[0011] More preferably, the skin repair composition comprises, by weight percentage, 8% to 10% grapefruit extract, 25% to 35% yeast / barley seed fermentation product filtrate, 0.3% to 0.7% licorice root extract, and the balance being butylene glycol.

[0012] In a second aspect, the present invention provides a method for using the above-mentioned skin repair composition, comprising the following steps: S1. Add the polyol of the formula amount into the reactor, heat to 40~60℃, stir and preheat at 100~300 rpm for 10~20 minutes to obtain the preheated polyol phase; S2. Under the condition of maintaining a temperature of 40~60℃, add the formula amount of yeast / barley seed fermentation product filtrate to the preheated polyol phase, and stir at 200~400 rpm for 5~15 minutes to fully disperse it and obtain the first mixed phase; S3. Add the prescribed amount of grapefruit extract to the first mixed phase and stir at 300-500 rpm for 10-20 minutes to obtain the second mixed phase; S4. Add the prescribed amount of Glycyrrhiza uralensis root extract to the second mixed phase, and stir at 200-400 rpm for 5-10 minutes to obtain the third mixed phase; S5. Cool the third mixed phase to 25~35℃, and under a vacuum of -0.06~-0.09 MPa, stir and degas at 100~200 rpm for 10~20 minutes to obtain the degassed mixture. S6. Allow the degassed mixture to stand and age for 12-24 hours at an aging temperature of 20-30°C to obtain the aged composition; S7. After filtration through a 200-400 mesh filter, the naringin content is tested to be no less than 5000 ppm and the pH value is 4.0-7.0. Then, the mixture is filled and discharged to obtain the skin repair composition.

[0013] Preferably, the yeast / barley seed fermentation product filtrate is prepared by the following steps: F1. Inoculate the activated yeast into a fermentation medium containing barley seeds and ferment at 25-35℃ for 24-72 hours to obtain the fermentation broth; F2. Centrifuge or coarsely filter the fermentation broth to remove the bacterial cells and large particulate residues, and obtain the initial filtrate. F3. The initial filtrate is filtered and sterilized through a microfiltration membrane with a pore size of 0.22~0.45 μm, and the filtrate is collected to obtain the yeast / barley seed fermentation product filtrate.

[0014] Thirdly, the present invention also provides the application of the above-mentioned skin repair composition or the skin repair composition prepared by the above-mentioned preparation method in the preparation of skin care products with skin barrier repair, anti-inflammatory or antioxidant effects, wherein the amount of the composition added to the skin care product is 0.5% to 2.0%.

[0015] Fourthly, the present invention also provides a skin repair formulation comprising the above-mentioned skin repair composition and cosmetically acceptable excipients, wherein the amount of the skin repair composition added is 0.5% to 2.0%.

[0016] Preferably, the total bacterial count of the composition is <100 CFU / g, and the total mold and yeast count is <10 CFU / g.

[0017] Compared with the prior art, the present invention has the following beneficial effects: This invention is the first to construct a ternary compound system using polyols as the continuous phase, consisting of yeast / barley seed fermentation product filtrate, grapefruit extract, and licorice root extract. This system solves the technical problems of flocculation and stratification and easy degradation of active ingredients in high-content fermentation filtrate in aqueous systems. It achieves excellent stability with a naringin retention rate of ≥93% under accelerated conditions of 40℃ for 90 days, a DPPH free radical scavenging rate of over 85%, and an LPS-induced NO generation inhibition rate of over 65%, exhibiting both excellent antioxidant and anti-inflammatory activities.

[0018] The composition of the present invention does not require the addition of preservatives and can exert good antibacterial effects in an anhydrous environment and with polyols.

[0019] This invention employs a seven-step preparation process, including step-by-step temperature-controlled feeding, vacuum degassing, static aging, and online quality control of active ingredient content, to ensure the consistency of efficacy and the physicochemical stability of different batches of products during long-term storage. Attached Figure Description

[0020] To more clearly illustrate the technical solution of the present invention, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 This is a flowchart illustrating the preparation process of the skin repair composition of the present invention.

[0022] Figure 2 Thermographs showing the physical stability of different compositions of the present invention.

[0023] Figure 3 This is a graph showing the change in naringin content under accelerated conditions at 40℃ according to the present invention.

[0024] Figure 4 This is a comparison chart of the NO generation inhibition rates of different compositions of the present invention.

[0025] Figure 5 This is a percutaneous permeation curve of isolated pig skin according to the present invention. Detailed Implementation

[0026] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to specific examples. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0027] In this invention, there are no particular limitations on the specific dispersion and stirring methods.

[0028] Unless otherwise specified, the experimental methods used in this invention are conventional methods; unless otherwise specified, the materials and reagents used are commercially available.

[0029] In this invention, the raw materials used include, but are not limited to, the following specifications: pomelo( CITRUS GRANDIS Fruit extract: Flavonoid content (calculated as naringin) ≥10%, derived from commercially available cosmetic raw materials.

[0030] Glycyrrhiza uralensis (Glycyrrhiza uralensis) GLYCYRRHIZA INFLATA Root extract: Licorice chalcone A content ≥5%, derived from commercially available cosmetic raw materials.

[0031] Yeast / barley seed fermentation product filtrate: solid content ≤3%, pH 4.5~5.5.

[0032] The yeast / barley seed fermentation product filtrate is prepared through, but is not limited to, the following steps: 1. Activated *Candida cruzi* (strain number: CICC 31748) was inoculated at a rate of 5% into a fermentation medium containing barley seeds, and fermented at 30°C for 60 hours to obtain the fermentation broth; the viable count of *Candida cruzi* was 1×10⁻⁶. 8 CFU / mL.

[0033] 2. Centrifuge or coarsely filter the fermentation broth to remove the bacterial cells and large particulate residues, and obtain the initial filtrate.

[0034] 3. The initial filtrate is filtered and sterilized through a microfiltration membrane with a pore size of 0.45 μm, and the filtrate is collected to obtain the yeast / barley seed fermentation product filtrate.

[0035] Butylene glycol: Cosmetic grade, purity ≥99%.

[0036] All the above raw materials meet the relevant standards for cosmetics. Other reagents and raw materials not specified are all cosmetic grade or analytical grade.

[0037] Example 1 A skin repair composition comprising, by weight percentage, 8.5% grapefruit extract, 0.5% licorice root extract, 30.0% yeast / barley seed ferment filtrate, and 61.0% butylene glycol.

[0038] like Figure 1 As shown, a method for preparing a skin repair composition includes the following steps: 1. Butanediol is added to a reaction vessel, heated to 50°C, and preheated by stirring at 200 rpm for 15 minutes to obtain the preheated polyol phase; 2. Under the condition of maintaining a temperature of 50℃, add the formula amount of yeast / barley seed fermentation product filtrate to the preheated polyol phase, and stir at 300 rpm for 10 minutes to fully disperse it and obtain the first mixed phase; 3. Add the prescribed amount of grapefruit extract to the first mixed phase and stir at 400 rpm for 15 minutes to obtain the second mixed phase; 4. Add the prescribed amount of Glycyrrhiza uralensis root extract to the second mixed phase, and stir at 300 rpm for 8 minutes to obtain the third mixed phase; 5. Cool the third mixed phase to 30°C, and under a vacuum of -0.08 MPa, stir and degas at 150 rpm for 15 minutes to obtain the degassed mixture. 6. Allow the degassed mixture to stand and age for 18 hours at a temperature of 25°C to obtain the aged composition; 7. Filter the aged composition through a 300-mesh filter, test the naringin content and pH value, and after passing the test, fill and discharge the material to obtain the skin repair composition.

[0039] The composition of this embodiment was tested and found to have a naringin content of 8030 ppm, a pH value of 5.0, a relative density (D 20 / 4) of 1.032, and a refractive index (N 20 / D) of 1.4160. The total bacterial count and total mold and yeast count of the composition of this embodiment were <10 CFU / g.

[0040] Example 2 A skin repair composition comprising, by weight percentage, 9.5% grapefruit fruit extract, 0.7% licorice root extract, 25.0% yeast / barley seed ferment filtrate, and 64.8% butylene glycol.

[0041] A method for preparing a skin repair composition includes the following steps: 1. Butanediol was added to the reaction vessel and heated to 45°C. It was stirred and preheated at 150 rpm for 18 minutes to obtain the preheated polyol phase. 2. Under the condition of maintaining a temperature of 45℃, add the formula amount of yeast / barley seed fermentation product filtrate to the preheated polyol phase, and stir at 250 rpm for 12 minutes to fully disperse it and obtain the first mixed phase; 3. Add the prescribed amount of grapefruit extract to the first mixed phase and stir at 350 rpm for 18 minutes to obtain the second mixed phase; 4. Add the prescribed amount of Glycyrrhiza uralensis root extract to the second mixed phase, and stir at 250 rpm for 10 minutes to obtain the third mixed phase; 5. Cool the third mixed phase to 28°C, and under a vacuum of -0.08 MPa, stir and degas at 120 rpm for 20 minutes to obtain the degassed mixture. 6. Allow the degassed mixture to stand and age for 24 hours at a temperature of 22°C to obtain the aged composition; 7. Filter the aged composition through a 300-mesh filter, test the naringin content and pH value, and after passing the test, fill and discharge the material to obtain the skin repair composition.

[0042] The composition of this embodiment was tested and found to have a naringin content of 7850 ppm, a pH of 5.2, a relative density (D 20 / 4) of 1.028, and a refractive index (N 20 / D) of 1.4145. The total bacterial count and total mold and yeast count of the composition were both <10 CFU / g.

[0043] Example 3 A skin repair composition comprising, by weight percentage, 7.5% grapefruit fruit extract, 0.3% licorice root extract, 40.0% yeast / barley seed ferment filtrate, and 52.2% butylene glycol.

[0044] A method for preparing a skin repair composition includes the following steps: 1. Butanediol is added to a reaction vessel, heated to 55°C, and preheated by stirring at 250 rpm for 12 minutes to obtain the preheated polyol phase; 2. Under the condition of maintaining a temperature of 55℃, add the formula amount of yeast / barley seed fermentation product filtrate to the preheated polyol phase, and stir at 350 rpm for 8 minutes to fully disperse it and obtain the first mixed phase; 3. Add the prescribed amount of grapefruit extract to the first mixed phase and stir at 450 rpm for 12 minutes to obtain the second mixed phase; 4. Add the prescribed amount of Glycyrrhiza uralensis root extract to the second mixed phase, and stir at 350 rpm for 6 minutes to obtain the third mixed phase; 5. Cool the third mixed phase to 32°C, and under a vacuum of -0.08 MPa, stir and degas at 180 rpm for 12 minutes to obtain the degassed mixture. 6. Allow the degassed mixture to stand and age for 14 hours at a temperature of 28°C to obtain the aged composition; 7. Filter the aged composition through a 300-mesh filter, test the naringin content and pH value, and after passing the test, fill and discharge the material to obtain the skin repair composition.

[0045] The composition of this embodiment was tested and found to contain 7620 ppm of naringin, have a pH of 4.9, a relative density (D 20 / 4) of 1.035, and a refractive index (N 20 / D) of 1.4185. The total bacterial count and total mold and yeast count of the composition were both <10 CFU / g.

[0046] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that grapefruit extract was not added, and the amount of butylene glycol was increased accordingly to make up to 100%, otherwise it was the same as Example 1.

[0047] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that Glycyrrhiza uralensis root extract was not added, and the amount of butylene glycol was increased accordingly to make up to 100%, otherwise it was the same as Example 1.

[0048] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that the content of yeast / barley seed fermentation product filtrate was reduced to 5.0%, and the amount of butanediol was increased accordingly to make up to 100%, otherwise it was the same as Example 1.

[0049] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that butanediol was replaced with an equal mass of deionized water (i.e., an aqueous system). The preparation method was adjusted to a conventional aqueous phase mixing method: deionized water was heated to 50°C, and yeast / barley seed fermentation product filtrate, grapefruit extract, and licorice root extract were added sequentially. The mixture was stirred for 30 minutes, cooled, and then discharged. Everything else was the same as in Example 1.

[0050] Comparative Example 5 The difference between Comparative Example 5 and Example 1 is that Comparative Example 5 is prepared by a simple mixing method, which is the same as that of Example 1.

[0051] According to the formula, all components are added to the reactor at once and stirred at 300 rpm for 30 minutes at room temperature (25℃). The product is obtained without step feeding, vacuum degassing, or static aging.

[0052] Test Example 1 Appearance and physical stability tests.

[0053] Experimental materials: Skin repair compositions prepared in Examples 1-3 and Comparative Examples 1-5.

[0054] Experimental methods: Room temperature storage: Dispense each sample into a 30 mL transparent glass bottle, seal, and store in a 25℃ incubator for 90 days. Observe the appearance (color, transparency, presence or absence of precipitation or stratification) on days 0, 30, 60, and 90.

[0055] High temperature acceleration: Each sample was stored in a 40℃ constant temperature chamber for 30 days, and the appearance changes were observed.

[0056] Freeze-thaw cycle: Place each sample in a -18℃ freezer for 24 hours, then thaw it at 25℃ for 24 hours. This is one cycle. Repeat three times. Observe the appearance after each cycle.

[0057] Table 1. Results of Appearance and Physical Stability Tests From Table 1 and Figure 2 It can be seen that Examples 1-3 and Comparative Examples 1-3 remained clear and transparent after 90 days at room temperature, 30 days at 40°C, and three freeze-thaw cycles, without precipitation or stratification, indicating that the anhydrous polyol system of the present invention has excellent physical stability. The stability of Comparative Example 4 decreased significantly, proving that removing the aqueous phase is key to improving stability. The stability of Comparative Example 5 was slightly lower than that of the Example groups, indicating that the stepwise feeding, vacuum degassing, and aging processes of the present invention contribute positively to long-term stability.

[0058] Test Example 2 Stability test of naringin content.

[0059] Experimental materials: Examples 1-3, Comparative Example 1, Comparative Example 4, Comparative Example 5.

[0060] Experimental methods: The naringin content in the samples was determined by high performance liquid chromatography (HPLC). Chromatographic conditions: C18 column (4.6 × 250 mm, 5 μm), mobile phase: acetonitrile-0.1% phosphoric acid aqueous solution (25:75), flow rate: 1.0 mL / min, detection wavelength: 283 nm, column temperature: 30℃. Samples were aliquoted and stored in a 40℃ incubator for 90 days. Naringin content was measured on days 0, 30, 60, and 90, and the retention rate was calculated as (content on day N / content on day 0 × 100%).

[0061] Table 2. Retention rate of naringin content (%) From Table 2 and Figure 3It can be seen that after 90 days of accelerated storage at 40°C, the naringin retention rate in Examples 1-3 was all above 93%, indicating that the anhydrous polyol system of the present invention has an excellent protective effect on naringin. In contrast, the retention rate in Comparative Example 4 was only 58.4% after 90 days, indicating severe degradation of naringin. The retention rate in Comparative Example 5 was 89.5%, lower than that in the Example groups, demonstrating that the preparation process of the present invention helps reduce the oxidative degradation of the active ingredient.

[0062] Test Example 3 pH stability test.

[0063] Experimental materials: Examples 1-3, Comparative Example 4, Comparative Example 5.

[0064] Experimental method: After each sample was dispensed, it was stored at 4℃, 25℃ and 40℃ for 90 days. The pH value was measured every 30 days using a pH meter (calibrated with standard buffer solution before each measurement). Each sample was measured 3 times and the average value was taken.

[0065] Table 3 pH value changes As shown in Table 3, the pH changes in Examples 1-3 after 90 days of storage at 40℃ did not exceed 0.1, indicating that the compositions have excellent acid-base stability. In Comparative Example 4, the pH continuously increased, with a change of up to 0.79, which may be related to microbial metabolism or component hydrolysis in the water. The pH change in Comparative Example 5 was -0.30, significantly greater than that of the Example groups, indicating that the preparation process of the present invention helps maintain the pH stability of the system.

[0066] Test Example 4 Microbial testing.

[0067] Experimental materials: Example 1, Comparative Example 4.

[0068] Experimental method: Referring to the microbial challenge test method in the "Cosmetic Safety Technical Specifications", a mixed bacterial suspension (containing Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger) was inoculated into the sample to make the initial bacterial count approximately 10^6~10^7 CFU / g. Samples were taken on days 0, 2, 7, 14, and 28 to determine the number of surviving bacteria.

[0069] Table 4. Microbial Challenge Test Results (Log CFU / g) As shown in Table 4, no viable bacteria were detected in Example 1 on the second day (<10 CFU / g), demonstrating excellent antibacterial effect without the need for additional preservatives. In Comparative Example 4, although the bacterial count decreased throughout the testing period, it remained at a high level, indicating that the aqueous system required preservatives and the antibacterial effect was still unsatisfactory.

[0070] Test Example 5 DPPH free radical scavenging experiment.

[0071] Experimental materials: Skin repair compositions prepared in Examples 1-3 and Comparative Examples 1-5. Positive control: Vitamin C (VC, 0.1 mg / mL). Blank control: Deionized water.

[0072] Experimental methods: Prepare DPPH free radical working solution (0.1 mM, dissolved in anhydrous ethanol). Take 2 mL of each sample, add 2 mL of DPPH working solution, mix well, and let stand in the dark for 30 minutes. Measure the absorbance value A at 517 nm. sample Simultaneously, the background absorbance A of the sample was measured. background (2 mL sample + 2 mL anhydrous ethanol) and blank control absorbance A control (2 mL deionized water + 2 mL DPPH working solution). Calculate the DPPH free radical scavenging rate according to the following formula (1).

[0073] (1).

[0074] Each sample was measured three times, and the average value was taken.

[0075] Table 5. DPPH Free Radical Scavenging Rate Test Results As shown in Table 5, the DPPH free radical scavenging rates of Examples 1-3 all reached over 85%, significantly higher than those of the comparative examples. Comparative Example 1, without grapefruit extract, had a scavenging rate of only 32.5%; Comparative Example 2, without licorice extract, had a scavenging rate of 51.8%, indicating that both grapefruit extract and *Glycyrrhiza uralensis* root extract are major contributors to the antioxidant activity of the compositions of this invention, and that they exhibit synergistic effects. Comparative Example 3, using a low-content fermentation filtrate, achieved a scavenging rate of 63.2%, lower than the example groups, indicating that a high-content yeast / barley seed fermentation product filtrate also has antioxidant activity. Although the scavenging rates of Comparative Examples 4 and 5 were higher than those of Comparative Examples 1-3, they were still lower than those of the example groups, indicating that the anhydrous polyol system and the preparation process of this invention help protect the antioxidant efficacy of the active ingredients.

[0076] Test Example 6 Experiment to inhibit NO generation.

[0077] Experimental materials: Skin repair compositions prepared in Examples 1-3 and Comparative Examples 1-5. Positive control: Dexamethasone (1 μM). Blank control: Culture medium containing an equal volume of serum-free medium. Cell line: Mouse macrophages RAW 264.7.

[0078] Experimental methods: RAW 264.7 cells were seeded in 96-well plates (1×10^5 cells / well) and cultured for 24 hours. Then, samples (diluted 500-fold, final concentration approximately 0.2%) were added for 1 hour of pretreatment, followed by stimulation with lipopolysaccharide (LPS, final concentration 1 μg / mL) for 24 hours. Cell supernatants were collected, and nitrite content was determined using the Griess reagent method, representing NO production. An LPS-stimulated group without samples was set as a model control (NO production was set to 100%). The NO inhibition rate was calculated using the following formula (2): Inhibition rate (%) = (NO amount in LPS model group - NO amount in sample group) / NO amount in LPS model group × 100% (2).

[0079] Each sample was measured three times, and the average value was taken.

[0080] Table 6. Results of NO generation inhibition rate test From Table 6 and Figure 4 It can be seen that the NO inhibition rates of Examples 1-3 all reached over 65%, indicating that the compositions of the present invention have good anti-inflammatory activity. Comparative Example 1 showed an inhibition rate of only 28.7%, and Comparative Example 2 showed an inhibition rate of 36.5%, indicating that both have anti-inflammatory effects, and the combined effect is significantly enhanced. Comparative Example 3, using a low-content fermentation filtrate, showed an inhibition rate of 45.3%, lower than the Example group, indicating that a high content of yeast / barley seed fermentation product filtrate plays an important role in enhancing anti-inflammatory efficacy. The inhibition rates of Comparative Examples 4 and 5 were 59.6% and 61.2%, respectively, slightly lower than the Example group, further verifying the positive significance of the anhydrous polyol system and specific preparation process of the present invention in maintaining efficacy.

[0081] Test Example 7 Transdermal permeation experiment of isolated pig skin.

[0082] Experimental materials: Skin model: Fresh excised pig skin (taken from the abdominal skin of a healthy pig approximately 6 months old), subcutaneous fat tissue removed, washed with physiological saline, frozen at -20℃, and thawed naturally before use.

[0083] Samples to be tested: Example 1, Comparative Example 4 (aqueous system), Comparative Example 5 (simple mixture).

[0084] Reference sample: 0.5% solution of naringin standard (purity ≥98%) (solvent: butanediol:water = 60:40).

[0085] Instrument: Franz diffusion cell (effective diffusion area approximately 1.77 cm²)2 (The receiving cell volume is approximately 12 mL); high-performance liquid chromatograph (HPLC, under the same conditions as test example 2).

[0086] Experimental methods: Thawed pigskin was fixed between the supply and receiving cells of a Franz diffusion cell, with the cuticle facing upwards. Phosphate-buffered saline (PBS) at pH 7.4 was added to the receiving cell as the receiving solution, and the cell was kept in a constant temperature water bath at 32°C with magnetic stirring (300 rpm). After equilibration for 30 minutes, 1.0 g of the test sample or control sample was added to the supply cell. At 2, 4, 6, 8, 10, 12, and 24 hours after addition, 1 mL samples were taken from the receiving cell, and an equal volume of fresh receiving solution was added. The samples were filtered through a 0.22 μm microporous membrane, and the naringin content was determined by HPLC. The cumulative permeate volume per unit area (μg / cm³) was calculated. 2 ).

[0087] Table 7. Cumulative permeation of naringin per unit area at different time points (μg / cm²) 2 )surface From Table 7 and Figure 5 It can be seen that in Example 1, the cumulative permeation of naringin reached 38.2 μg / cm³ within 24 hours. 2 The concentration of naringin was significantly higher than that of Comparative Example 4, Comparative Example 5, and the control sample. This indicates that the anhydrous polyol system is more conducive to the transdermal absorption of naringin compared to the aqueous system. This is presumably because polyols have a certain penetration-enhancing effect and avoid the dilution and retention of active ingredients by water. Furthermore, the stepwise feeding, vacuum degassing, and aging processes of this invention help to uniformly disperse the active ingredients in the matrix, thereby improving their bioavailability. At the same time, the higher transdermal accumulation of naringin indicates that the composition of this invention can more effectively deliver the active ingredients to the deep layers of the skin, thereby exerting antioxidant, anti-inflammatory, and skin-repairing effects.

[0088] In summary, the skin repair composition provided by this invention significantly outperforms the comparative examples in terms of physical stability, naringin content stability, pH stability, microbial stability, and efficacy in antioxidation, anti-inflammation, and transdermal absorption of active ingredients. Specifically, the anhydrous polyol system, the specific ratio of the three components (grapefruit extract, licorice root extract, and high-content yeast / barley seed fermentation product filtrate), and the stepwise feeding, vacuum degassing, and static aging process achieve long-term stable storage and high efficacy under high active ingredient load. In particular, the in vitro transdermal permeation experiment using pig skin confirms that the composition of this invention can significantly promote the transdermal absorption of active ingredients, providing direct evidence for its application in the field of skin repair.

[0089] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A skin repair composition, characterized in that, By mass percentage, it includes 5% to 10% grapefruit extract, 0.1% to 2% licorice root extract, 15% to 50% yeast / barley seed ferment filtrate, and the balance polyols. The composition contains not less than 5000 ppm of naringin.

2. The skin repair composition according to claim 1, characterized in that, The polyol is butanediol.

3. The skin repair composition according to claim 1, characterized in that, The pH value of the composition is 4.0~7.0 under direct measurement at 25°C.

4. The skin repair composition according to claim 1, characterized in that, The composition has a relative density of 1.000~1.100 and a refractive index of 1.4050~1.4250.

5. The skin repair composition according to claim 1, characterized in that, By weight percentage, it includes 8%–10% grapefruit extract, 25%–35% yeast / barley seed fermentation product filtrate, 0.3%–0.7% licorice root extract, and the balance butylene glycol.

6. A method for preparing the skin repair composition according to any one of claims 1 to 5, characterized in that, Includes the following steps: S1. Add the polyol of the formula amount into the reactor, heat to 40~60℃, stir and preheat at 100~300 rpm for 10~20 minutes to obtain the preheated polyol phase; S2. Under the condition of maintaining a temperature of 40~60℃, add the formula amount of yeast / barley seed fermentation product filtrate to the preheated polyol phase, and stir at 200~400 rpm for 5~15 minutes to fully disperse it and obtain the first mixed phase; S3. Add the prescribed amount of grapefruit extract to the first mixed phase and stir at 300-500 rpm for 10-20 minutes to obtain the second mixed phase; S4. Add the prescribed amount of Glycyrrhiza uralensis root extract to the second mixed phase, and stir at 200-400 rpm for 5-10 minutes to obtain the third mixed phase; S5. Cool the third mixed phase to 25~35℃, and under a vacuum of -0.06~-0.09 MPa, stir and degas at 100~200 rpm for 10~20 minutes to obtain the degassed mixture. S6. Allow the degassed mixture to stand and age for 12-24 hours at an aging temperature of 20-30°C to obtain the aged composition; S7. After filtration through a 200-400 mesh filter, the naringin content is tested to be no less than 5000 ppm and the pH value is 4.0-7.

0. Then, the mixture is filled and discharged to obtain the skin repair composition.

7. A method for preparing the yeast / barley seed fermentation product filtrate in the skin repair composition according to any one of claims 1 to 5, characterized in that, Includes the following steps: F1. Inoculate the activated yeast into a fermentation medium containing barley seeds and ferment at 25-35℃ for 24-72 hours to obtain the fermentation broth; F2. Centrifuge or coarsely filter the fermentation broth to remove the bacterial cells and large particulate residues, and obtain the initial filtrate. F3. The initial filtrate is filtered and sterilized through a microfiltration membrane with a pore size of 0.22~0.45 μm, and the filtrate is collected to obtain the yeast / barley seed fermentation product filtrate.

8. The use of the skin repair composition according to any one of claims 1 to 5 or the skin repair composition prepared by the method according to claim 6 in the preparation of skin care products having skin barrier repair, anti-inflammatory, or antioxidant effects, characterized in that, The composition is added to skin care products at a rate of 0.5% to 2.0%.

9. A skin repair preparation, characterized in that, The product comprises the skin repair composition according to any one of claims 1 to 5 and cosmetically acceptable excipients, wherein the amount of the skin repair composition added is 0.5% to 2.0%.

10. The skin repair composition according to claim 1, characterized in that, The total bacterial count of the composition is <100 CFU / g, and the total mold and yeast count is <10 CFU / g.

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