A topical dressing with both moisturizing and anti-inflammatory properties and its preparation method

Abstract

This invention relates to the field of dressing technology and discloses a topical dressing that combines moisturizing and anti-inflammatory properties, as well as its preparation method. The dressing is made from a biomass matrix, an embedded antibacterial agent, peony seed oil, chrysanthemum oil, and dihydroammyl-anaminobenzoic acid, mixed together. The embedded antibacterial agent is a mesoporous bioactive glass with a pectin molecular layer on its surface, loaded with antibacterial functional agents. It can slowly release quaternary ammonium salt antibacterial agents, improving the antibacterial effect of the dressing. Simultaneously, it can synergistically work with the mesoporous bioactive glass to form an orderly sequence of "debridement followed by anti-inflammatory and repair promotion," reducing scab formation and mechanical damage, and providing a suitable hydration environment for cell migration. Furthermore, the specific addition of peony seed oil, chrysanthemum oil, and dihydroammyl-anaminobenzoic acid in this invention can provide auxiliary moisturizing and anti-inflammatory effects.

Description

Technical Field

[0001] This invention relates to the field of dressing technology, specifically to a topical dressing that combines moisturizing and anti-inflammatory properties and its preparation method. Background Technology

[0002] Skin wound repair and regeneration is a fundamental and important issue in clinical medicine. Maintaining a suitable local microenvironment is crucial at each stage of wound healing. An ideal wound dressing has evolved from its initial function of simple physical coverage and isolation to actively regulating the wound environment, such as maintaining adequate moisture, controlling inflammatory responses, and preventing microbial invasion.

[0003] Current clinically used dressings, such as dry gauze and cotton pads, play a role in managing exudate and providing physical protection, but they have significant functional limitations. They easily adhere to newly formed granulation tissue or dried scabs, leading to secondary tearing injuries during dressing changes and increasing patient discomfort. More importantly, these dressings fail to provide the necessary moist healing environment for the wound; instead, excessive dryness may delay epidermal cell migration and proliferation, hindering the healing process.

[0004] To address these issues, modern moist dressings such as hydrocolloids, hydrogels, and foam dressings have been developed. These products perform better in maintaining wound moisture and absorbing exudate. However, the vast majority of them still lack active bioactive functions. For example, many synthetic polymer-based dressings (such as polyurethane films and polyacrylic acid hydrogels) do not possess anti-inflammatory or antibacterial capabilities themselves and usually require additional loading with antibiotics, silver ions, or chemically synthesized anti-inflammatory drugs to achieve the corresponding activity. This approach presents new challenges: antibiotic overuse may induce bacterial resistance; long-term or high-dose use of silver ions poses a potential risk of cytotoxicity; and chemical anti-inflammatory drugs (such as steroids or non-steroidal anti-inflammatory drugs) may have adverse effects on newly formed tissue.

[0005] Meanwhile, polymeric materials derived from renewable biomass resources, such as starch and cellulose, are becoming popular matrix materials for constructing next-generation smart dressings due to their good biocompatibility, biodegradability, wide availability, and low cost. However, natural starch or cellulose itself has significant limitations in wound dressing applications: firstly, most of them lack intrinsic anti-inflammatory activity and cannot effectively intervene in the excessive inflammatory response in the early stages of a wound; secondly, unmodified materials have weak antibacterial properties and are difficult to provide independent protection in the easily infected wound environment. In existing technologies, although some studies have attempted to simply physically mix these materials with antibacterial / anti-inflammatory components, they often face problems such as uncontrollable release of active ingredients, short duration of action, and difficulty in synergistic effects between different functions.

[0006] Therefore, how to develop a topical dressing that can simultaneously achieve long-lasting moisturizing, highly effective anti-inflammatory and broad-spectrum antibacterial effects without relying on traditional antibiotics or chemically synthesized anti-inflammatory drugs, using biomass starch or biomass cellulose as the basic framework and through innovative structural design and functionalization strategies, while ensuring its safety in clinical use, remains a key technical challenge that urgently needs to be solved in this field.

[0007] The present invention aims to overcome the shortcomings of the prior art and provide a novel external dressing with excellent moisturizing properties, significant anti-inflammatory properties and intrinsic antibacterial properties, as well as its preparation method. Summary of the Invention

[0008] In order to solve the problems mentioned in the background art, the purpose of this invention is to provide a topical dressing that combines moisturizing and anti-inflammatory properties and a method for preparing the same.

[0009] The objective of this invention can be achieved through the following technical solutions: A topical dressing that combines moisturizing and anti-inflammatory properties is made from the following ingredients measured in parts by weight: Biomass matrix: 46-58 parts; Encapsulated antibacterial agent: 2.5-6.4 parts; Peony seed oil: 5-8 parts; Bozhou chrysanthemum oil: 5-10 parts; Dihydroamaryl-o-aminobenzoic acid: 3-5 parts.

[0010] As a further aspect of the present invention, the biomass matrix is ​​at least one of starch, cellulose, guar gum, or gelatin.

[0011] As a further aspect of the present invention, the encapsulated and supported antibacterial agent is prepared by the following method: Step 1: Add mesoporous bioactive glass to deionized water, stir and disperse evenly to form a uniform dispersion; add antibacterial functional agent to deionized water, stir and mix evenly to prepare a functional solution; Step 2: Under continuous stirring, add the functional liquid to the dispersion. After the addition is complete, control the ultrasonic frequency to 80-120kHz and ultrasonically treat for 1-2 hours. Filter to separate the solid material, wash and vacuum dry to obtain the supported bioactive glass. Step 3: Add the supported bioactive glass to deionized water and stir to disperse it evenly. Then add pectin to the dispersion. After the addition is complete, stir continuously at 70-80℃ for 4-6 hours. Then stop heating, cool down and discharge the material. Collect the solid material, wash and vacuum dry it to obtain the encapsulated supported antibacterial agent.

[0012] As a further aspect of the present invention, in step one, the mesoporous bioactive glass is prepared by the following method: Add hexadecyltrimethylammonium bromide to deionized water and stir at 30-40℃ until homogeneous. Then add tetraethyl orthosilicate and calcium nitrate, and adjust the pH to 2 with nitric acid. After hydrolysis for 3-5 hours, add ammonium dihydrogen phosphate and adjust the pH to 10 with ammonia. Continue stirring and maintaining the temperature for 9-12 hours. Then raise the temperature to 90-100℃ and maintain the temperature for 24-48 hours. Centrifuge to collect the solid material, and then wash and freeze-dry it to obtain the final product.

[0013] As a further aspect of the present invention, in step one, the mass fraction of the dispersion is 5-15%.

[0014] As a further aspect of the present invention, in step one, the antibacterial functional agent is any one of (2,3-dihydroxypropyl)trimethylammonium chloride, bis(2-hydroxyethyl)dimethylammonium chloride, or di(hydroxyethyl)methyldodecylammonium chloride.

[0015] As a further aspect of the present invention, in step one, the mass fraction of the functional liquid is 3-6%.

[0016] As a further aspect of the present invention, in step two, the volume ratio of the functional liquid to the dispersion liquid is 2-3:1.

[0017] As a further aspect of the present invention, in step three, the mass ratio of the supported bioactive glass to pectin is 1:2-4.

[0018] It should be noted that in the above technical solution, a bioactive glass with a rich porous structure, namely mesoporous bioactive glass, is first prepared using a template method. Because it initially exhibits negative charge in aqueous solution, it can electrostatically attract and adsorb antibacterial functional agents, significantly increasing the concentration of antibacterial functional agents around the mesoporous bioactive glass. Under ultrasonic action, the antibacterial functional agents can pass through the pore port barrier of the mesoporous bioactive glass, enter the pore interior, and be loaded within the pores. Simultaneously, the mesoporous bioactive glass... The surface can also adsorb a large number of antibacterial functional agents, forming a loaded bioactive glass. Then, the negatively charged pectin further aggregates and adsorbs around the loaded bioactive glass through electrostatic interaction. Then, the carboxyl groups in the pectin structure and the hydroxyl groups in the structure of the antibacterial functional agent loaded on the surface of the bioactive glass generate hydrogen bonds. Therefore, under the dual action of electrostatic adsorption and hydrogen bonding, a pectin molecular layer is coated on the surface of the mesoporous bioactive glass, and a mesoporous bioactive glass loaded with antibacterial functional agents and with a pectin molecular layer on the surface is obtained, that is, a loaded antibacterial agent is embedded.

[0019] A method for preparing a topical dressing that combines moisturizing and anti-inflammatory properties includes the following steps: Step 1: Weigh and prepare all ingredients according to the recipe, and set aside for later use; The second step is to add all the raw materials into a container and stir them together until a uniform material is formed, then discharge the material.

[0020] The beneficial effects of this invention are: This invention prepares mesoporous bioactive glass loaded with antibacterial functional agents and having a pectin molecular layer on its surface, serving as an embedded, loaded antibacterial agent. On one hand, the pectin molecular layer, acting as a polysaccharide interface, effectively improves the hydrophilic compatibility between the inorganic carrier mesoporous bioactive glass and the biomass matrix. On the other hand, the spatial confinement of the mesoporous channels and the swelling barrier of the pectin layer jointly inhibit the initial burst release of the quaternary ammonium salt antibacterial functional agent, achieving a long-lasting and stable antibacterial release, while simultaneously delaying the rapid dissolution of bioactive glass ions, resulting in a synergistic effect of anti-inflammatory and antibacterial actions over time.

[0021] When the dressing is applied to the wound, the pectin layer swells more rapidly in acidic or hyperexudative environments, leading to increased release of quaternary ammonium salt antibacterial agents, achieving on-demand antibacterial action. Release slows down in normally healing tissue, avoiding unnecessary drug exposure. Simultaneously, pectin itself has immunomodulatory effects, and its gel layer absorbs exudate, maintains wound moisture, reduces scab formation and mechanical damage, and provides a suitable hydration environment for cell migration. Furthermore, the mesoporous bioactive glass inhibits the expression of pro-inflammatory factors while promoting the secretion of vascular endothelial growth factor. The sustained-release properties extend the anti-inflammatory effect beyond the inflammatory phase, completely overlapping with the antibacterial window of the quaternary ammonium salt antibacterial agents, forming a well-ordered "debridement-anti-inflammatory-promoting repair" sequence.

[0022] This invention specifically adds peony seed oil and chrysanthemum oil as moisturizing ingredients, which synergistically enhance the moisturizing effect of the dressing with the biomass matrix and the encapsulated antibacterial agent. At the same time, the addition of dihydroamaryl-o-aminobenzoic acid plays an auxiliary role in anti-inflammatory effect.

[0023] Of course, any product implementing this invention does not necessarily need to achieve all of the advantages described above at the same time. Detailed Implementation

[0024] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0025] Preparation Example Preparation of encapsulated antibacterial agents: Step A: Add 12g of hexadecyltrimethylammonium bromide to deionized water and stir at 35°C until well mixed. Then add 3.5g of tetraethyl orthosilicate and 3.2g of calcium nitrate, and adjust the pH to 2 with nitric acid. After hydrolysis for 4 hours, add 0.34g of ammonium dihydrogen phosphate and adjust the pH to 10 with ammonia. Continue stirring and maintaining the temperature for 12 hours. Then raise the temperature to 95°C and maintain the temperature for 48 hours. Centrifuge to collect the solid material, wash and freeze-dry to obtain mesoporous bioactive glass. Step B: Add mesoporous bioactive glass to deionized water, stir and disperse evenly to form a uniform dispersion with a mass fraction of 12%; add (2,3-dihydroxypropyl)trimethylammonium chloride to deionized water, stir and mix evenly to prepare a functional solution with a mass fraction of 5%. Step C: Under continuous stirring, add 35 mL of functional liquid to 15 mL of dispersion. After the addition is complete, control the ultrasonic frequency to 100 kHz and ultrasonically treat for 2 hours. Filter to separate the solid material, wash and vacuum dry to obtain supported bioactive glass. Step D: Add 1.8g of the supported bioactive glass to deionized water and stir to disperse evenly. Then add 6.5g of pectin to the dispersion. After the addition is complete, stir continuously at 75℃ for 4 hours, then stop heating, cool down and discharge the material. Collect the solid material, wash and vacuum dry it to obtain the encapsulated supported antibacterial agent.

[0026] Example 1 A topical dressing that combines moisturizing and anti-inflammatory properties is made from the following ingredients measured in parts by weight: Guar gum: 46 parts; Encapsulated antibacterial agent: 2.5 parts; Bozhou peony seed oil: 5 parts; Bozhou chrysanthemum oil: 5 parts; Dihydroamaryl-o-aminobenzoic acid: 3 parts.

[0027] The preparation method of the dressing includes the following steps: Step 1: Weigh and prepare all ingredients according to the recipe, and set aside for later use; The second step is to add all the raw materials into a container and stir them together until a uniform material is formed, then discharge the material.

[0028] The preparation method of the encapsulated and supported antibacterial agent is shown in the preparation example, and the same applies to the following.

[0029] Example 2 A topical dressing that combines moisturizing and anti-inflammatory properties is made from the following ingredients measured in parts by weight: Guar gum: 50 parts; Encapsulated antibacterial agent: 6 parts; Peony seed oil: 6 parts; Bozhou chrysanthemum oil: 8 parts; Dihydroamaryl-o-aminobenzoic acid: 4 parts.

[0030] The preparation method of the dressing includes the following steps: Step 1: Weigh and prepare all ingredients according to the recipe, and set aside for later use; The second step is to add all the raw materials into a container and stir them together until a uniform material is formed, then discharge the material.

[0031] Example 3 A topical dressing that combines moisturizing and anti-inflammatory properties is made from the following ingredients measured in parts by weight: Guar gum: 58 parts; Encapsulated antibacterial agent: 6.4 parts; Peony seed oil: 8 parts; Bozhou chrysanthemum oil: 10 parts; Dihydroamaryl-o-aminobenzoic acid: 5 parts.

[0032] The preparation method of the dressing includes the following steps: Step 1: Weigh and prepare all ingredients according to the recipe, and set aside for later use; The second step is to add all the raw materials into a container and stir them together until a uniform material is formed, then discharge the material.

[0033] Comparative Example 1 A topical dressing that combines moisturizing and anti-inflammatory properties is made from the following ingredients measured in parts by weight: Guar gum: 50 parts; Supported bioactive glass: 6 parts; Peony seed oil: 6 parts; Bozhou chrysanthemum oil: 8 parts; Dihydroamaryl-o-aminobenzoic acid: 4 parts.

[0034] The preparation method of the dressing includes the following steps: Step 1: Weigh and prepare all ingredients according to the recipe, and set aside for later use; The second step is to add all the raw materials into a container and stir them together until a uniform material is formed, then discharge the material.

[0035] The preparation method of the supported bioactive glass is shown in the preparation example.

[0036] Comparative Example 2 A topical dressing that combines moisturizing and anti-inflammatory properties is made from the following ingredients measured in parts by weight: Guar gum: 50 parts; Mesoporous bioactive glass: 6 parts; Peony seed oil: 6 parts; Bozhou chrysanthemum oil: 8 parts; Dihydroamaryl-o-aminobenzoic acid: 4 parts.

[0037] The preparation method of the dressing includes the following steps: Step 1: Weigh and prepare all ingredients according to the recipe, and set aside for later use; The second step is to add all the raw materials into a container and stir them together until a uniform material is formed, then discharge the material.

[0038] The preparation method of mesoporous bioactive glass is shown in the preparation example.

[0039] Comparative Example 3 A topical dressing that combines moisturizing and anti-inflammatory properties is made from the following ingredients measured in parts by weight: Guar gum: 50 parts; Peony seed oil: 6 parts; Bozhou chrysanthemum oil: 8 parts; Dihydroamaryl-o-aminobenzoic acid: 4 parts.

[0040] The preparation method of the dressing includes the following steps: Step 1: Weigh and prepare all ingredients according to the recipe, and set aside for later use; The second step is to add all the raw materials into a container and stir them together until a uniform material is formed, then discharge the material.

[0041] Test case ① Weigh out m (g) of each dressing sample from the examples and comparative examples, pour it into water, remove it after it is saturated with adsorption, filter out the saturated sample, and drain it until no more water drips out. Record the mass at this time as n (g). Use the formula (nm) / m to calculate the water absorption ratio and evaluate the moisturizing performance of the dressing. ② The dressings from the examples and comparative examples were pressed into test samples with a diameter of 10 mm, left to stand at room temperature for 2 months, and then coated on the surface of nutrient agar medium with a Staphylococcus aureus suspension at a concentration of 10%. 5 CFU / mL, then the test sample was placed on nutrient agar medium and incubated at 37°C for 12 hours before the diameter of the inhibition zone was measured. ③ Seventy mice weighing 20±2g were randomly divided into seven groups. All mice had their back fur shaved, and a 1cm×1cm wound was created on the back of each mouse using a laser. Dressings prepared according to Examples 1-3 and Comparative Examples 1-3 of this invention were applied to the wound surface, and the wound healing phenomenon was recorded. The test results are shown in the table below: The test results are recorded in the table below:

[0042] This document uses specific examples to illustrate the principles and implementation methods of the present invention. The descriptions of these embodiments are merely to aid in understanding the method and core ideas of the present invention, including the best mode, and to enable any person skilled in the art to practice the present invention, including manufacturing and using any device or system, and implementing any combined method. It should be noted that those skilled in the art can make various improvements and modifications to the present invention without departing from its principles, and these improvements and modifications also fall within the scope of protection of the claims. The scope of protection of this patent is defined by the claims and may include other embodiments that can be conceived by those skilled in the art. If these other embodiments have structural elements similar to those expressed in the claims, or if they include equivalent structural elements that are not substantially different from those expressed in the claims, then these other embodiments should also be included within the scope of the claims.

[0043] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A topical dressing that combines moisturizing and anti-inflammatory properties, characterized in that, It is made from the following raw materials, measured in parts by weight: Biomass matrix: 46-58 parts; Encapsulated antibacterial agent: 2.5-6.4 parts; Peony seed oil: 5-8 parts; Bozhou chrysanthemum oil: 5-10 parts; Dihydroamaryl-o-aminobenzoic acid: 3-5 parts.

2. The topical dressing with both moisturizing and anti-inflammatory properties according to claim 1, characterized in that, The biomass matrix is ​​at least one of starch, cellulose, guar gum, or gelatin.

3. The topical dressing with both moisturizing and anti-inflammatory properties according to claim 1, characterized in that, The encapsulated and loaded antibacterial agent is prepared using the following method: Step 1: Add mesoporous bioactive glass to deionized water, stir and disperse evenly to form a uniform dispersion; add antibacterial functional agent to deionized water, stir and mix evenly to prepare a functional solution; Step 2: Under continuous stirring, add the functional liquid to the dispersion. After the addition is complete, control the ultrasonic frequency to 80-120kHz and ultrasonically treat for 1-2 hours. Filter to separate the solid material, wash and vacuum dry to obtain the supported bioactive glass. Step 3: Add the supported bioactive glass to deionized water and stir to disperse it evenly. Then add pectin to the dispersion. After the addition is complete, stir continuously at 70-80℃ for 4-6 hours. Then stop heating, cool down and discharge the material. Collect the solid material, wash and vacuum dry it to obtain the encapsulated supported antibacterial agent.

4. A topical dressing with both moisturizing and anti-inflammatory properties according to claim 3, characterized in that, In step one, the mesoporous bioactive glass is prepared using the following method: Add hexadecyltrimethylammonium bromide to deionized water and stir at 30-40℃ until homogeneous. Then add tetraethyl orthosilicate and calcium nitrate, and adjust the pH to 2 with nitric acid. After hydrolysis for 3-5 hours, add ammonium dihydrogen phosphate and adjust the pH to 10 with ammonia. Continue stirring and maintaining the temperature for 9-12 hours. Then raise the temperature to 90-100℃ and maintain the temperature for 24-48 hours. Centrifuge to collect the solid material, and then wash and freeze-dry it to obtain the final product.

5. A topical dressing with both moisturizing and anti-inflammatory properties according to claim 3, characterized in that, In step one, the mass fraction of the dispersion is 5-15%.

6. A topical dressing with both moisturizing and anti-inflammatory properties according to claim 3, characterized in that, In step one, the antibacterial functional agent is any one of (2,3-dihydroxypropyl)trimethylammonium chloride, bis(2-hydroxyethyl)dimethylammonium chloride, or di(hydroxyethyl)methyldodecylammonium chloride.

7. A topical dressing with both moisturizing and anti-inflammatory properties according to claim 3, characterized in that, In step one, the mass fraction of the functional liquid is 3-6%.

8. A topical dressing with both moisturizing and anti-inflammatory properties according to claim 3, characterized in that, In step two, the volume ratio of the functional liquid to the dispersion is 2-3:

1.

9. A topical dressing with both moisturizing and anti-inflammatory properties according to claim 3, characterized in that, In step three, the mass ratio of the supported bioactive glass to pectin is 1:2-4.

10. A method for preparing a topical dressing that combines moisturizing and anti-inflammatory properties as described in claim 1, characterized in that, Includes the following steps: Step 1: Weigh and prepare all ingredients according to the recipe, and set aside for later use; The second step is to add all the raw materials into a container and stir them together until a uniform material is formed, then discharge the material.

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