Special fertilizer for continuous cropping sugar beet paper tube seedling bed, preparation method and application

Abstract

This invention relates to the field of fertilizer technology for seedbeds, specifically a fertilizer for continuous-cropping sugar beet seedling beds using paper tube cultivation, its preparation method, and its application. The preparation steps include: S1. Mixing corn stalks, soybean meal, mushroom compost, sweet clover, and lignite powder in a mixer with water, followed by three-stage fermentation to obtain a fermentation product; S2. Drying the fermentation product, sieving it, and then mixing it evenly with a functional modifier and biochemical potassium humate to obtain a functional organic base material; S3. Adding vermiculite, bentonite, and modified microbial agents to the functional organic base material, mixing it evenly, and then sieving it to obtain the fertilizer for continuous-cropping sugar beet seedling beds using paper tube cultivation. This invention significantly improves the germination rate, root vitality, and post-transplant recovery speed of sugar beet seedbeds through the synergistic effect of the functional modifier, modified microbial agents, and antibacterial modifier.

Description

Technical Field

[0001] This invention relates to the field of fertilizer technology for seedbeds, specifically to fertilizer for seedbeds used in continuous cropping sugar beet paper tube seedling cultivation, its preparation method, and its application. Background Technology

[0002] Sugar beets are an important sugar crop in my country, especially in major producing areas such as Inner Mongolia and Xinjiang. With the large-scale development of the sugar industry, land resources are limited, and the area under continuous cropping of sugar beets has exceeded 30%. Continuous cropping obstacles lead to a 7.4-28.2% decrease in sugar content and a 0.5-2 degree Brix reduction in sugar beet yield, and a significant increase in the incidence of soil-borne diseases such as root rot and damping-off. Paper-tube seedling transplanting technology is a key agronomic measure to address insufficient accumulated temperature, extend the growing season, and increase sugar yield in northern sugar beets. Although the seedling period only accounts for about 1 / 10 of the total growing season, the quality of the seedbed soil directly determines the stress resistance, root development foundation, and final yield of the sugar beets after transplanting.

[0003] Currently, there has been relevant research on seedbed soil and specialized fertilizer technology for sugar beet paper tube seedling cultivation, but existing technologies still have the following technical pain points and shortcomings: The separation of fertilizer and functional components limits synergistic effects: Current seedbed soil preparation technologies rely on farmers or factories temporarily and physically mixing "chemical fertilizers / special fertilizers," "organic fertilizers (sheep manure)," and "microbial agents." Due to the diverse sources, unstable salt content, and pH values ​​of organic materials such as decomposed sheep manure, the survival rate and colonization of exogenously added beneficial microorganisms such as Bacillus subtilis are often low in the microenvironment of the paper tube, failing to fully exert the "bacterial-based disease control" effect of the microbial agent.

[0004] Misalignment between nutrient release and root absorption during the seedling stage: Existing organic-inorganic fertilizers are mostly mixtures of chemical compound fertilizers and simple organic matter. In the alternating wet and dry environment of frequent watering in paper tube seedling cultivation, nutrients are leached quickly, and seedling hypocotyls are easily burned due to excessively high local salt concentrations. In addition, traditional seedbed soil lacks precise regulation for the specific needs of continuously cropped sugar beet seedlings (such as sensitivity to molybdenum and the need for high phosphorus to promote root growth), resulting in problems such as yellowing cotyledons, weak root vitality, and long recovery period after transplanting.

[0005] There is a lack of physical structural improvements tailored to the characteristics of paper tube seedlings: Paper tube seedling cultivation requires the seedbed soil to remain loose and non-compacted while still moist, ensuring the soil column does not crumble during transplanting. Existing technologies largely rely on organic fertilizers and the natural properties of the seedbed soil, without specifically regulating the soil's colloid and micro-aggregate structure. Under continuous cropping conditions, the organic matter in the seedbed soil is rapidly depleted, leading to a deterioration of its physical structure. This results in increased soil compaction inside the paper tube after watering, restricting root penetration and making root breakage or "tube jamming" more likely during transplanting.

[0006] Therefore, developing a compound seedbed fertilizer that integrates slow release of organic nutrients, enhancement of specific microorganisms, and soil structure improvement functions, specifically designed for the continuous cropping sugar beet paper tube seedling environment, is of great practical significance for overcoming the problems of poor component synergy, weak seedling disease control, and unstable physical structure in existing technologies, and further improving the quality of sugar beet seedlings and their resistance to continuous cropping obstacles. Summary of the Invention

[0007] In view of the shortcomings of the existing technology, the purpose of this invention is to provide a special fertilizer for continuous cropping sugar beet paper tube seedling beds, its preparation method and application.

[0008] To achieve the above objectives, the present invention provides the following technical solution: The preparation method of special fertilizer for paper tube seedling beds of continuously cropped sugar beets includes the following preparation steps: S1. By weight, 30-40 parts corn stalks, 15-20 parts soybean meal, 10-15 parts mushroom bran, 10-15 parts sweet clover, and 5-8 parts lignite powder are put into a mixer and mixed. Water is added to a moisture content of 60-65%. After three stages of fermentation, the fermentation product is obtained. S2. Dry the fermentation product obtained in step S1 to a moisture content of 15-18%, pass it through a 7-8 mm sieve, and mix it evenly with 8-10 parts of functional modifier and 2-3 parts of biochemical potassium humate to obtain functional organic base material. S3. Add 6-10 parts vermiculite, 4-6 parts bentonite and 0.4-0.6 parts modified microbial agent to the functional organic base material obtained in step S2, mix evenly and pass through a 2-3 mm sieve to obtain a special fertilizer for continuous cropping beet paper tube seedling bed. The preparation of functional modifiers includes the following steps: S11. By weight, mix 3-5 parts fish bone powder, 0.2-0.3 parts citric acid and 3-5 parts water, stir until a paste is formed, and keep warm in a water bath at 45-48℃ for 1-2 hours to obtain the first-grade mixture; S12. Mix 3-5 parts of kelp residue, 0.05-0.1 parts of ammonium molybdate, 0.02-0.04 parts of disodium ethylenediaminetetraacetate and 3-5 parts of water, and stir evenly to obtain a secondary mixture; S13. Mix the primary and secondary mixtures obtained in steps S11 and S12, dry them at 55-60℃ until the moisture content is 5-8%, and pulverize them through a 100-mesh sieve to obtain the functional modifier.

[0009] Preferably, the preparation of the modified microbial agent includes the following steps: S21. By weight, mix 8-10 parts of bacterial fermentation broth, 15-18 parts of diatomaceous earth and 12-15 parts of modified adsorption carrier, and stir at 50-100 r / min for 20-30 min to adsorb. S22. The mixed bacterial solution obtained in step S21 is dried at 35-40℃ until the water content is 3-5%, and then pulverized to finally obtain the modified microbial agent.

[0010] Preferably, the preparation of the modified adsorbent carrier includes the following steps: S211. By mass, add 0.3-0.5 parts of KH-550 to 2-3 parts of 75% ethanol solution, and hydrolyze for 10-15 minutes to obtain hydrolysate; S212. Spray the hydrolysate obtained in step S211 onto a mixture of 8-10 parts rice husk charcoal powder and 5-8 parts sepiolite powder while stirring. After spraying, dry at 100-105℃ for 1-2 hours. S213. Add 1-2 parts of sodium carboxymethyl cellulose, 0.5-0.8 parts of anhydrous calcium chloride and 4-6 parts of water to the mixture obtained in step S212, and stir until it is loose and in the form of rice grains, and finally obtain the modified adsorption carrier.

[0011] Preferably, the fermentation broth is composed of *Azotobacter chrysogenum*, *Bacillus mucilaginosus*, *Bacillus megaterium*, and *Trichoderma harzianum* in a mass ratio of 1:2:2:1.

[0012] Preferably, the first stage of the three-stage fermentation is natural heating. When the temperature inside the pile reaches 50-55℃, the pile is turned once using a turner. The second stage controls the temperature at 60-70℃ and maintains it for 7-9 days, turning the pile once every 2 days. The third stage controls the temperature at 30-40℃, adds 3-5 parts of antibacterial modifier, turns the pile evenly, and continues to pile for 3-4 days.

[0013] Preferably, the preparation of the antibacterial modifier includes the following steps: S31. By weight, mix 1-2 parts of Sophora flavescens powder, 2-3 parts of tea seed cake powder and 5-7 parts of water and stir well; S32. Add 0.1-0.3 parts of sodium bicarbonate and 0.2-0.4 parts of chitosan quaternary ammonium salt to the mixture obtained in step S31, disperse it ultrasonically at 35-40℃ for 20-30 min, and pass it through a 200-mesh sieve. S33. Add 2-3 parts of rice husk charcoal powder to the slurry after sieving, mix evenly, dry at 45-50℃ for 4-5 hours, and pulverize through an 80-mesh sieve to finally obtain the antibacterial modifier.

[0014] Preferably, the drying temperature in step S2 is 50-60℃.

[0015] Preferably, the ultrasonic dispersion frequency in step S32 is 40 kHz.

[0016] The special fertilizer for paper tube seedling beds of continuously cropped sugar beets is prepared by the above preparation method.

[0017] Application of special fertilizer for beet paper tube seedling raising in continuous cropping.

[0018] Compared with the prior art, the beneficial effects of the present invention are: 1. The modified adsorption carrier of this invention enhances the survival and activity retention of modified microbial agents. Surface modification firmly fixes the bacterial strains to the inner wall of the carrier pores, effectively resisting bacterial loss and high-temperature inactivation under alternating wet and dry conditions in the seedbed soil. The microgel structure formed by the cross-linked network of sepiolite fibers and sodium-calcium carboxymethyl cellulose ions endows the carrier with excellent water-retention and slow-release properties, providing a continuously suitable micro-domain water activity environment for functional microorganisms under conditions of frequent watering in paper tube seedling cultivation.

[0019] 2. The specific nutrients provided by the functional modifier of this invention induce the root system to secrete chemotactic substances. The modified microbial agent, protected by a carrier, responds rapidly and colonizes. The antibacterial modifier eliminates competitive pressure from rhizosphere pathogens. The colonized functional bacteria further secrete auxins and siderophores, promoting root development and activating the sugar beet systemic resistance. Through the synergistic effect of the functional modifier, modified microbial agent, and antibacterial modifier, the emergence rate, root vigor, and post-transplant recovery speed of sugar beets in the seedbed are significantly improved. Attached Figure Description

[0020] Figure 1 This is a flowchart illustrating the preparation process of the special fertilizer for continuous cropping sugar beet paper tube seedling bed of the present invention. Figure 2 This is a process flow diagram for preparing the functional modifier of the present invention; Figure 3 This is a flow chart of the preparation process of the modified microbial agent of the present invention; Figure 4 This is a flow chart illustrating the preparation process of the antibacterial modifier of the present invention. Figure 5 This is a flow chart of the preparation process of the modified adsorption carrier of the present invention. Detailed Implementation

[0021] The present invention will now be clearly and completely described in conjunction with embodiments thereof. Obviously, the described embodiments are merely some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0022] Please see Figures 1-5 The present invention provides a technical solution: Example 1 Preparation method of special fertilizer for paper tube seedling beds of continuously cropped sugar beets: Before preparing the special fertilizer for continuous cropping sugar beet paper tube seedling beds, the following steps are taken: Preparation of modified adsorption carriers, functional modifiers, modified microbial agents, and antibacterial modifiers. The preparation of the modified adsorbent carrier includes the following steps: S211. Add 0.3g of KH-550 to 2g of 75% ethanol solution and hydrolyze for 10min to obtain hydrolysate; S212. Spray the hydrolysate obtained in step S211 onto a mixture of 8g rice husk charcoal powder and 5g sepiolite powder while stirring. After spraying, dry at 100℃ for 1 hour. S213. Add 1g sodium carboxymethyl cellulose, 0.5g anhydrous calcium chloride and 4g water to the mixture obtained in step S212, and stir until it is loose and in the form of rice grains, and finally obtain the modified adsorption carrier.

[0023] The preparation of functional modifiers includes the following steps: S11. Mix 3g fish bone powder, 0.2g citric acid and 3g water, stir until a paste is formed, and keep warm in a water bath at 45℃ for 1 hour to obtain the first-stage mixture; S12. Mix 3g of kelp residue, 0.05g of ammonium molybdate, 0.02g of disodium ethylenediaminetetraacetate and 3g of water, and stir evenly to obtain a secondary mixture; S13. Mix the primary and secondary mixtures obtained in steps S11 and S12, dry them at 55°C until the moisture content is 5%, and pulverize them through a 100-mesh sieve to obtain the functional modifier.

[0024] The preparation of modified microbial agents includes the following steps: S21. Mix 8g of bacterial fermentation broth (composed of Azotobacter chrysogenum, Bacillus mucilaginosus, Bacillus megaterium, and Trichoderma harzianum in a mass ratio of 1:2:2:1), 15g of diatomaceous earth, and 12g of modified adsorption carrier, and stir at 50r / min for 20min for adsorption. S22. The mixed bacterial solution obtained in step S21 is dried at 35°C until the water content is 3%, and then pulverized to finally obtain the modified microbial agent.

[0025] The preparation of the antibacterial modifier includes the following steps: S31. Mix 1g of Sophora flavescens powder, 2g of tea seed cake powder and 5g of water and stir well; S32. Add 0.1g sodium bicarbonate and 0.2g chitosan quaternary ammonium salt to the mixture obtained in step S31, and ultrasonically disperse it at 35℃ and 40kHz for 20min, then pass it through a 200-mesh sieve. S33. Add 2g of rice husk charcoal powder to the slurry after sieving, mix evenly, dry at 45℃ for 4h, and pulverize through an 80-mesh sieve to finally obtain the antibacterial modifier.

[0026] S1. Mix 30g corn stalks, 15g soybean meal, 10g mushroom compost, 10g sweet clover, and 5g lignite powder in a mixer, add water to a moisture content of 60%, and ferment in three stages (the first stage is natural heating; when the temperature inside the pile reaches 50℃, turn the pile once with a turner; the second stage controls the temperature at 60℃ and maintains it for 7 days, turning the pile every 2 days; the third stage controls the temperature at 30℃, adds 3g of antibacterial modifier, turns the pile evenly, and continues to pile for 3 days) to obtain the fermentation product; S2. The fermentation product obtained in step S1 is dried at 50°C to a moisture content of 15%, passed through a 7mm sieve, and then mixed evenly with 8g of functional modifier and 2g of biochemical potassium humate to obtain functional organic base material. S3. Add 6g vermiculite, 4g bentonite and 0.4g modified microbial agent to the functional organic base material obtained in step S2, mix evenly and pass through a 2mm sieve to obtain a special fertilizer for continuous cropping beet paper tube seedling bed.

[0027] Example 2 Preparation method of special fertilizer for paper tube seedling beds of continuously cropped sugar beets: Before preparing the special fertilizer for continuous cropping sugar beet paper tube seedling beds, the following steps are taken: Preparation of modified adsorption carriers, functional modifiers, modified microbial agents, and antibacterial modifiers. The preparation of the modified adsorbent carrier includes the following steps: S211. Add 0.5g of KH-550 to 3g of 75% ethanol solution and hydrolyze for 15min to obtain hydrolysate; S212. Spray the hydrolysate obtained in step S211 onto a mixture of 10g rice husk charcoal powder and 8g sepiolite powder while stirring. After spraying, dry at 105℃ for 2 hours. S213. Add 2g sodium carboxymethyl cellulose, 0.8g anhydrous calcium chloride and 6g water to the mixture obtained in step S212, and stir until it is loose and in the form of rice grains, and finally obtain the modified adsorption carrier.

[0028] The preparation of functional modifiers includes the following steps: S11. Mix 5g fish bone powder, 0.3g citric acid and 5g water, stir until a paste is formed, and keep warm in a water bath at 48℃ for 2 hours to obtain the first-stage mixture; S12. Mix 5g of kelp residue, 0.1g of ammonium molybdate, 0.04g of disodium ethylenediaminetetraacetate and 5g of water, and stir evenly to obtain a secondary mixture; S13. Mix the primary and secondary mixtures obtained in steps S11 and S12, dry them at 60°C until the moisture content is 8%, and pulverize them through a 100-mesh sieve to obtain the functional modifier.

[0029] The preparation of modified microbial agents includes the following steps: S21. Mix 10g of bacterial fermentation broth (composed of Azotobacter chrysogenum, Bacillus mucilaginosus, Bacillus megaterium, and Trichoderma harzianum in a mass ratio of 1:2:2:1), 18g of diatomaceous earth, and 15g of modified adsorption carrier, and stir at 100r / min for 30min for adsorption. S22. The mixed bacterial solution obtained in step S21 is dried at 40°C until the water content is 5%, and then pulverized to finally obtain the modified microbial agent.

[0030] The preparation of the antibacterial modifier includes the following steps: S31. Mix 2g of Sophora flavescens powder, 3g of tea seed cake powder and 7g of water and stir well; S32. Add 0.3g sodium bicarbonate and 0.4g chitosan quaternary ammonium salt to the mixture obtained in step S31, and ultrasonically disperse it at 40℃ and 40kHz for 30min, and then pass it through a 200-mesh sieve. S33. Add 3g of rice husk charcoal powder to the slurry after sieving, mix evenly, dry at 50℃ for 5h, and pulverize through an 80-mesh sieve to finally obtain the antibacterial modifier.

[0031] S1. Mix 40g corn stalks, 20g soybean meal, 15g mushroom compost, 15g sweet clover, and 8g lignite powder in a mixer, add water to a moisture content of 65%, and ferment in three stages (the first stage is natural heating; when the temperature inside the pile reaches 55℃, turn the pile once with a turner; the second stage controls the temperature at 70℃ and maintains it for 9 days, turning the pile every 2 days; the third stage controls the temperature at 40℃, adds 5g of antibacterial modifier, turns the pile evenly, and continues to pile for 4 days) to obtain the fermentation product; S2. The fermentation product obtained in step S1 is dried at 60°C to a moisture content of 18%, passed through an 8mm sieve, and then mixed evenly with 10g of functional modifier and 3g of biochemical potassium humate to obtain functional organic base material. S3. Add 10g vermiculite, 6g bentonite and 0.6g modified microbial agent to the functional organic base material obtained in step S2, mix evenly and pass through a 3mm sieve to obtain a special fertilizer for continuous cropping beet paper tube seedling bed.

[0032] Example 3 Preparation method of special fertilizer for paper tube seedling beds of continuously cropped sugar beets: Before preparing the special fertilizer for continuous cropping sugar beet paper tube seedling beds, the following steps are taken: Preparation of modified adsorption carriers, functional modifiers, modified microbial agents, and antibacterial modifiers. The preparation of the modified adsorbent carrier includes the following steps: S211. Add 0.4g of KH-550 to 2.5g of 75% ethanol solution and hydrolyze for 12min to obtain hydrolysate; S212. Spray the hydrolysate obtained in step S211 onto a mixture of 9g rice husk charcoal powder and 6g sepiolite powder while stirring. After spraying, dry at 102℃ for 1.5h. S213. Add 1.5g sodium carboxymethyl cellulose, 0.6g anhydrous calcium chloride and 5g water to the mixture obtained in step S212, and stir until it is loose and in the form of rice grains, and finally obtain the modified adsorption carrier.

[0033] The preparation of functional modifiers includes the following steps: S11. Mix 4g fish bone powder, 0.25g citric acid and 4g water, stir until a paste is formed, and keep warm in a water bath at 46℃ for 1.5h to obtain the first-stage mixture; S12. Mix 4g of kelp residue, 0.06g of ammonium molybdate, 0.03g of disodium ethylenediaminetetraacetate and 4g of water, and stir evenly to obtain a secondary mixture; S13. Mix the primary and secondary mixtures obtained in steps S11 and S12, dry them at 56°C until the moisture content is 6%, and pulverize them through a 100-mesh sieve to obtain the functional modifier.

[0034] The preparation of modified microbial agents includes the following steps: S21. Mix 9g of bacterial fermentation broth (composed of Azotobacter chrysogenum, Bacillus mucilaginosus, Bacillus megaterium, and Trichoderma harzianum in a mass ratio of 1:2:2:1), 16g of diatomaceous earth, and 13g of modified adsorption carrier, and stir at 60r / min for 22min for adsorption. S22. The mixed bacterial solution obtained in step S21 is dried at 36°C until the water content is 4%, and then pulverized to finally obtain the modified microbial agent.

[0035] The preparation of the antibacterial modifier includes the following steps: S31. Mix 1.5g of Sophora flavescens powder, 2.5g of tea seed cake powder and 6g of water and stir well; S32. Add 0.2g sodium bicarbonate and 0.3g chitosan quaternary ammonium salt to the mixture obtained in step S31, and ultrasonically disperse it at 36℃ and 40kHz for 22min, and then pass it through a 200-mesh sieve. S33. Add 2.5g of rice husk charcoal powder to the slurry after sieving, mix evenly, dry at 46℃ for 4.5h, and pulverize through an 80-mesh sieve to finally obtain the antibacterial modifier.

[0036] S1. Mix 32g corn stalks, 16g soybean meal, 12g mushroom compost, 12g sweet clover, and 6g lignite powder in a mixer, add water to a moisture content of 62%, and ferment in three stages (the first stage is natural heating; when the temperature inside the pile reaches 52℃, turn the pile once with a turner; the second stage controls the temperature at 62℃ and maintains it for 8 days, turning the pile every 2 days; the third stage controls the temperature at 32℃, adds 4g of antibacterial modifier, turns the pile evenly, and continues to pile for 3 days) to obtain the fermentation product; S2. The fermentation product obtained in step S1 is dried at 52°C to a moisture content of 16%, passed through a 7mm sieve, and then mixed evenly with 9g of functional modifier and 2.5g of biochemical potassium humate to obtain functional organic base material. S3. Add 7g vermiculite, 5g bentonite and 0.5g modified microbial agent to the functional organic base material obtained in step S2, mix evenly and pass through a 2mm sieve to obtain a special fertilizer for continuous cropping beet paper tube seedling bed.

[0037] Comparative Example 1 The only difference between Comparative Example 1 and Example 1 is that no functional modifier was added in this comparative example; the other steps are exactly the same in Comparative Example 1 and Example 1.

[0038] Comparative Example 2 The only difference between Comparative Example 2 and Example 1 is that no antibacterial modifier was added in this comparative example; the other steps are exactly the same in Comparative Example 2 and Example 1.

[0039] Comparative Example 3 The only difference between Comparative Example 3 and Example 1 is that the modified adsorption carrier in this comparative example is replaced with rice husk charcoal powder. The other steps are exactly the same in Comparative Example 3 and Example 1.

[0040] Comparative Example 4 Comparative Example 4 used commercially available conventional beet seedling fertilizer as a control.

[0041] Performance testing: This invention uses seedling paper tubes with a diameter of 1.9cm and a height of 13cm, containing 1400 tubes per volume. Topsoil from corn stubble that has not been planted with sugar beets for over 5 years (passed through a 6mm sieve, moisture content 15-16%) is used as the basic seedling soil. The seedbed soil for each paper tube is prepared according to the following ratio: 60kg of basic seedling soil per tube, and 0.5kg of the tested fertilizer (obtained in Examples 1-3 and Comparative Examples 1-3) per tube. During seedling management, water thoroughly after sowing (water temperature 20-25℃), do not water before emergence, and supplement water according to soil moisture after emergence. Maintain warmth from sowing to emergence, with nighttime temperatures ≥5℃; after emergence, maintain daytime temperatures of 15-20℃ and nighttime temperatures ≥5℃. After emergence, when daytime temperatures exceed 25℃, open the film for ventilation in a sheltered location; harden the seedlings by keeping the greenhouse open day and night for 7 days before transplanting. Spray with potassium dihydrogen phosphate (20g / 10L water) 4 days before transplanting, and water thoroughly 1 day before transplanting. The transplanting fields were all sugar beet fields that had been continuously cropped for 3 years. The final test data are shown in Tables 1-4 below: Table 1. Performance test data of paper tube seedling raising and transplanting. Table 2 Dynamic changes in beet plant height after transplanting Table 3. Dynamic changes in dry matter accumulation of sugar beets after transplanting Table 4. Root rot disease and changes in yield and quality of sugar beets during harvest. As shown in Tables 1-4, the embodiment exhibits significantly better performance than the comparative embodiment in terms of germination rate, seedling growth, root vigor, damping-off disease incidence, and transplant survival rate in paper tube seedling cultivation, and is also significantly superior to commercially available conventional beet seedling fertilizers. The germination rate and seedling growth indicate that the present invention, through the synergistic effect of citric acid activating fish bone meal phosphate and kelp residue alginic acid in the functional modifier, effectively promotes seed germination energy supply and hypocotyl elongation. Furthermore, the modified adsorption carrier ensures rapid colonization of functional bacteria in the early sowing stage, producing auxin-like substances that stimulate seedling growth. Root vigor verifies the protective effect of the modified adsorption carrier on functional bacteria and the crucial role of the functional modifier in providing specific nutrients for beet seedling root development. The transplant survival rate and disease control demonstrate that the matrine-tea saponin-chitosan quaternary ammonium salt complex in the antibacterial modifier inhibits the germination of pathogens such as Rhizoctonia solani during the seedling stage, while the robust root system promoted by the functional modifier also improves the plant's resistance to adverse conditions. The synergistic effect of these two factors establishes a dual barrier of "antibacterial inhibition and growth promotion" in the seedbed, resulting in rapid seedling recovery, strong resistance to adverse conditions, and fewer diseases after transplanting. After transplanting, the example significantly outperformed the control group in terms of growth (plant height, dry matter accumulation) and other performance aspects throughout the entire growth cycle of continuously cropped sugar beets, and this effect was even more pronounced than that of commercially available conventional sugar beet seedling fertilizers. The final yield, sugar content, and sugar production at harvest fully demonstrate that the synergistic effect of the functional modifier (precise nutrient regulation), modified microbial agents (rhizosphere microecological reconstruction), and antibacterial modifier (pathogen inhibition) is the core mechanism for overcoming the obstacles of continuous cropping of sugar beets and improving product quality.

[0042] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a special fertilizer for continuous-cropping sugar beet paper tube seedling beds, characterized in that, The preparation steps include the following: S1. By weight, 30-40 parts corn stalks, 15-20 parts soybean meal, 10-15 parts mushroom bran, 10-15 parts sweet clover, and 5-8 parts lignite powder are put into a mixer and mixed. Water is added to a moisture content of 60-65%. After three stages of fermentation, the fermentation product is obtained. S2. Dry the fermentation product obtained in step S1 to a moisture content of 15-18%, pass it through a 7-8 mm sieve, and mix it evenly with 8-10 parts of functional modifier and 2-3 parts of biochemical potassium humate to obtain functional organic base material. S3. Add 6-10 parts vermiculite, 4-6 parts bentonite and 0.4-0.6 parts modified microbial agent to the functional organic base material obtained in step S2, mix evenly and pass through a 2-3 mm sieve to obtain a special fertilizer for continuous cropping beet paper tube seedling bed. The preparation of the functional modifier includes the following steps: S11. By weight, mix 3-5 parts fish bone powder, 0.2-0.3 parts citric acid and 3-5 parts water, stir until a paste is formed, and keep warm in a water bath at 45-48℃ for 1-2 hours to obtain the first-grade mixture; S12. Mix 3-5 parts of kelp residue, 0.05-0.1 parts of ammonium molybdate, 0.02-0.04 parts of disodium ethylenediaminetetraacetate and 3-5 parts of water, and stir evenly to obtain a secondary mixture; S13. Mix the primary and secondary mixtures obtained in steps S11 and S12, dry them at 55-60℃ until the moisture content is 5-8%, and pulverize them through a 100-mesh sieve to obtain the functional modifier.

2. The method for preparing the special fertilizer for continuous cropping sugar beet paper tube seedling beds according to claim 1, characterized in that, The preparation of the modified microbial agent includes the following steps: S21. By weight, mix 8-10 parts of bacterial fermentation broth, 15-18 parts of diatomaceous earth and 12-15 parts of modified adsorption carrier, and stir at 50-100 r / min for 20-30 min to adsorb. S22. The mixed bacterial solution obtained in step S21 is dried at 35-40℃ until the water content is 3-5%, and then pulverized to finally obtain the modified microbial agent.

3. The method for preparing the special fertilizer for continuous cropping sugar beet paper tube seedling beds according to claim 2, characterized in that, The preparation of the modified adsorbent carrier includes the following steps: S211. By mass, add 0.3-0.5 parts of KH-550 to 2-3 parts of 75% ethanol solution, and hydrolyze for 10-15 minutes to obtain hydrolysate; S212. Spray the hydrolysate obtained in step S211 onto a mixture of 8-10 parts rice husk charcoal powder and 5-8 parts sepiolite powder while stirring. After spraying, dry at 100-105℃ for 1-2 hours. S213. Add 1-2 parts of sodium carboxymethyl cellulose, 0.5-0.8 parts of anhydrous calcium chloride and 4-6 parts of water to the mixture obtained in step S212, and stir until it is loose and in the form of rice grains, and finally obtain the modified adsorption carrier.

4. The method for preparing the special fertilizer for continuous cropping sugar beet paper tube seedling bed according to claim 2, characterized in that, The fermentation broth is composed of *Azotobacter chrysogenum*, *Bacillus mucilaginosus*, *Bacillus megaterium*, and *Trichoderma harzianum*, in a mass ratio of 1:2:2:

1.

5. The method for preparing the special fertilizer for continuous cropping sugar beet paper tube seedling beds according to claim 1, characterized in that, The first stage of the three-stage fermentation is natural heating. When the temperature inside the pile reaches 50-55℃, the pile is turned once using a turner. The second stage controls the temperature at 60-70℃ and maintains it for 7-9 days, turning the pile every 2 days. The third stage controls the temperature at 30-40℃, adds 3-5 parts of antibacterial modifier, turns the pile evenly, and continues to pile for 3-4 days.

6. The method for preparing the special fertilizer for continuous cropping sugar beet paper tube seedling bed according to claim 5, characterized in that, The preparation of the antibacterial modifier includes the following steps: S31. By weight, mix 1-2 parts of Sophora flavescens powder, 2-3 parts of tea seed cake powder and 5-7 parts of water and stir well; S32. Add 0.1-0.3 parts of sodium bicarbonate and 0.2-0.4 parts of chitosan quaternary ammonium salt to the mixture obtained in step S31, disperse it ultrasonically at 35-40℃ for 20-30 min, and pass it through a 200-mesh sieve. S33. Add 2-3 parts of rice husk charcoal powder to the slurry after sieving, mix evenly, dry at 45-50℃ for 4-5 hours, and pulverize through an 80-mesh sieve to finally obtain the antibacterial modifier.

7. The method for preparing the special fertilizer for continuous cropping sugar beet paper tube seedling bed according to claim 1, characterized in that, The drying temperature in step S2 is 50-60℃.

8. The method for preparing the special fertilizer for continuous cropping sugar beet paper tube seedling bed according to claim 6, characterized in that, The ultrasonic dispersion frequency in step S32 is 40 kHz.

9. A special fertilizer for continuous cropping sugar beet paper tube seedling beds, characterized in that, It is prepared by the preparation method described in any one of claims 1-8.

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