polypeptide

Abstract

This invention discloses polypeptides, nucleotides encoding these polypeptides, and methods for producing these polypeptides. This invention also discloses detergent compositions comprising these polypeptides, laundry methods, and uses of these polypeptides.

Description

[0001] This application is a divisional application of the invention patent application filed on October 8, 2016, with application number 201680057711.X and invention title "Polypeptide".

[0002] References to sequence lists

[0003] This application contains a sequence list in computer-readable form, which is incorporated herein by reference. Technical Field

[0004] This invention relates to novel polypeptides having deoxyribonuclease (DNase) activity, nucleotides encoding these polypeptides, and methods for producing these polypeptides. The invention also relates to detergent compositions containing DNase, laundry methods, and uses of DNase. Background Technology

[0005] In many natural, industrial, and medical environments, microorganisms typically survive by attaching to surfaces and encapsulating themselves with extracellular substances including biopolymers and macromolecules. The layer produced by microorganisms that have undergone mucus encapsulation is called a biofilm. Biofilms are the primary mode of bacterial growth in the natural environment, and bacteria growing in biofilms exhibit unique physiological characteristics. Compared to their planktonic counterparts, bacteria in biofilms are more resistant to antibiotics, UV radiation, detergents, and host immune responses.

[0006] Biofilms can include one or more microorganisms, including Gram-positive and Gram-negative bacteria, algae, protozoa, and / or yeasts or filamentous fungi and viruses and / or bacteriophages. Examples of problematic biofilms are dental plaque, infections on medical implants, and initial contamination on ship hulls. Biofilms are attributed to the pathogenesis of many human infections and are a significant problem in industry in terms of biofouling of exposed surfaces, where biofilm colonization can form a fundamental component of local ecosystems capable of disrupting and interfering with industrial processes and components.

[0007] When clothing (like T-shirts or sportswear) is used, it is exposed to bacteria from the user's body and the rest of the environment in which it is used. Some of these bacteria are able to adhere to the clothing and form a biofilm on it. The presence of bacteria means that the clothing becomes sticky, and therefore dirt adheres to the sticky areas. This dirt has been shown to be difficult to remove with commercially available detergent compositions. Furthermore, when very dirty clothing is washed with less dirty clothing, the dirt present in the detergent tends to adhere to the biofilm. As a result, the clothing appears "dirtier" after washing than before. In addition, these bacteria are a source of unpleasant odors, producing an unpleasant smell after use on clothing. This unpleasant odor (malodor) is difficult to remove and can persist even after washing. The cause of this unpleasant odor is the adhesion of bacteria to the surface of textiles. Because of this adhesion, the bacteria remain even after washing and continue to be a source of unpleasant odors.

[0008] International patent applications WO 2011 / 098579 (University of Newcastle) and WO2014 / 087011 (Novozymes A / S) relate to deoxyribonuclease compounds and methods for biofilm disruption and prevention. Summary of the Invention

[0009] This invention relates to novel polypeptides having DNase (deoxyribonuclease) activity and polynucleotides encoding these novel polypeptides. One aspect of the invention relates to a composition comprising at least 0.002 ppm of a DNase-active polypeptide, wherein the polypeptide comprises the motif HXXP, where H is histidine, P is proline, and X is any amino acid, wherein the composition further comprises: one or more polyols, preferably selected from glycerol, (mono, di, or tri) propylene glycol, ethylene glycol, polyethylene glycol, sugar alcohols, sorbitol, mannitol, erythritol, galactitol, inositol, xylitol, and ribitol, and / or

[0010] ii. Optionally, one or more enzymes, preferably selected from proteases, amylases, or lipases. iii. Optionally, one or more surfactants, preferably selected from anionic and nonionic surfactants. iv. Optionally one or more polymers; Another aspect of the invention relates to particles comprising i. A core, comprising a polypeptide with DNase activity, and optionally, ii. Coating, which consists of one or more layers surrounding the core.

[0011] In one aspect of the invention, the particle comprises a polypeptide having DNase activity, and wherein the polypeptide comprises one or more motifs selected from the motifs [T / D / S][G / N]PQL (SEQ ID NO 198), [G / T]Y[D / S][R / K / L] (SEQ ID NO 199), [E / D / H]H[I / V / L / F / M]X[P / A / S] (SEQ ID NO 200), [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201) and C[D / N]T[A / R] (SEQ ID NO: 202), and wherein the particle comprises a core and a coating, the core comprising the polypeptide.

[0012] One aspect of the invention relates to a composition comprising a polypeptide having DNase activity, wherein the polypeptide comprises one or more motifs selected from the motifs [T / D / S][G / N]PQL (SEQ ID NO 198), [G / T]Y[D / S][R / K / L] (SEQ ID NO 199), [E / D / H]H[I / V / L / F / M]X[P / A / S] (SEQ ID NO 200), [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201) and C[D / N]T[A / R] (SEQ ID NO: 202).

[0013] In one aspect of the invention, a composition is provided, wherein the polypeptide having DNase activity belongs to the GYS clade and comprises one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205).

[0014] In one aspect, the composition comprises a polypeptide having DNase activity, wherein the polypeptide comprises one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), and wherein the polypeptide comprises an amino acid sequence selected from the group consisting of or having at least 80% sequence identity with the group consisting of, substantially consisting of, or consisting of, an amino acid sequence selected from the group consisting of or having at least 80% sequence identity with the group consisting of, the group consisting of: SEQ ID NO 8, SEQ ID NO 9, SEQ ID NO 11, SEQ ID NO 12, SEQ ID NO 13, SEQ ID NO 14, SEQ ID NO 15, SEQ ID NO 16, SEQ ID NO 17, SEQ ID NO 18, SEQ ID NO 19, SEQ ID NO 20, SEQ ID NO 21, SEQ ID NO 22, SEQ ID NO 23, SEQ ID NO 53. SEQ ID NO 56, SEQ ID NO 59, SEQ ID NO 62, SEQ ID NO 65, SEQ ID NO 68, SEQ ID NO 71, SEQ ID NO 74, SEQ ID NO 77 and SEQ ID NO 80.

[0015] In one aspect, the composition comprises a polypeptide with DNA enzyme activity, the polypeptide belonging to the NAWK clade and containing one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) or NPQL (SEQ ID NO: 207).

[0016] In one aspect, the composition comprises a polypeptide having DNase activity, wherein the polypeptide comprises one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) or NPQL (SEQ ID NO: 207), and wherein the polypeptide comprises an amino acid sequence selected from the group consisting of or having at least 80% sequence identity with the group consisting of, substantially consisting of, an amino acid sequence selected from the group consisting of or having at least 80% sequence identity with the group consisting of, the group consisting of, SEQ ID NO 83, SEQ ID NO 86, SEQ ID NO 89, SEQ ID NO 92, SEQ ID NO 95, SEQ ID NO 98, SEQ ID NO 101, SEQ ID NO 104, SEQ ID NO 107, SEQ ID NO 110, SEQ ID NO 113, SEQ ID NO 116 and SEQ ID NO 119.

[0017] In one aspect, the composition comprises a polypeptide with DNA enzyme activity, and the polypeptide belongs to the KNAW clade and contains one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209).

[0018] In one aspect, the composition comprises a polypeptide having DNase activity, wherein the polypeptide comprises P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), and wherein the polypeptide comprises an amino acid sequence selected from the group consisting of or having at least 80% sequence identity with the group consisting of amino acid sequences selected from the group consisting of or having at least 80% sequence identity with the group consisting of the following: SEQ ID NO 122, SEQ ID NO 125, SEQ ID NO 128, SEQ ID NO 131, SEQ ID NO 134, SEQ ID NO 137, SEQ ID NO 140, SEQ ID NO 143, SEQ ID NO 146, SEQ ID NO 149, SEQ ID NO 152, SEQ ID NO 155 and SEQ ID NO 158.

[0019] In one respect, the composition is a cleaning composition, such as a laundry or dishwashing composition.

[0020] One aspect of the present invention relates to a polypeptide having DNase activity, wherein the polypeptide comprises the motif HXXP, wherein H is histidine and wherein P is proline and X is any amino acid.

[0021] On one hand, the polypeptide with DNase activity comprises one or more motifs selected from the group consisting of: [T / D / S][G / N]PQL (SEQ ID NO 198), [G / T]Y[D / S][R / K / L] (SEQ ID NO 199), [E / D / H]H[I / V / L / F / M]X[P / A / S] (SEQ ID NO 200), [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201), and C[D / N]T[A / R] (SEQ ID NO: 202).

[0022] In one aspect of the invention, the polypeptide with DNase activity belongs to the GYS clade and contains one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205).

[0023] In one respect, the polypeptide is selected from the group consisting of the polypeptides shown in SEQ ID NO 8, SEQ ID NO 9, SEQ ID NO 11, SEQ ID NO 12, SEQ ID NO 13, SEQ ID NO 14, SEQ ID NO 15, SEQ ID NO 16, SEQ ID NO 17, SEQ ID NO 18, SEQ ID NO 19, SEQ ID NO 20, SEQ ID NO 21, SEQ ID NO 22, SEQ ID NO 23, SEQ ID NO 53, SEQ ID NO 56, SEQ ID NO 59, SEQ ID NO 62, SEQ ID NO 65, SEQ ID NO 68, SEQ ID NO 71, SEQ ID NO 74, SEQ ID NO 77 and SEQ ID NO 80, or polypeptides having at least 98% sequence identity with them.

[0024] In one aspect of the invention, the polypeptide with DNase activity belongs to the NAWK clade and wherein the polypeptide contains one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) or NPQL (SEQ ID NO: 207).

[0025] In one aspect, the polypeptide comprises either the motif [V / I]PL[S / A]NAWK (SEQ ID NO: 206) or NPQL (SEQ ID NO: 207), and is selected from the group consisting of the polypeptides shown in SEQ ID NO 83, SEQ ID NO 86, SEQ ID NO 89, SEQ ID NO 92, SEQ ID NO 95, SEQ ID NO 98, SEQ ID NO 101, SEQ ID NO 104, SEQ ID NO 107, SEQ ID NO 110, SEQ ID NO 113, SEQ ID NO 116 and SEQ ID NO 119, or polypeptides having at least 95% sequence identity with them.

[0026] In one aspect of the invention, the polypeptide having DNase activity belongs to the KNAW clade and contains one or two motifs selected from the following: motif P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209).

[0027] In one aspect, the polypeptide comprises the motif P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209) and is selected from the group consisting of the polypeptides shown in SEQ ID NO 122, SEQ ID NO 125, SEQ ID NO 128, SEQ ID NO 131, SEQ ID NO 134, SEQ ID NO 137, SEQ ID NO 140, SEQ ID NO 143, SEQ ID NO 146, SEQ ID NO 149, SEQ ID NO 152, SEQ ID NO 155 and SEQ ID NO 158 or polypeptides having at least 98% sequence identity with them.

[0028] One aspect of the invention relates to a polynucleotide encoding the polypeptide of the invention. The invention further relates to a nucleic acid construct or expression vector comprising the polynucleotide. The invention further relates to a host cell comprising the polypeptide of the invention.

[0029] One aspect relates to the use of the polypeptides of the present invention for reducing or removing biofilms from articles (e.g., textiles), preferably in cleaning processes (e.g., laundry).

[0030] One aspect relates to a method for generating the polypeptide of the present invention, the method comprising: (a) Culture recombinant host cells under conditions conducive to the production of the polypeptide; and (b) The polypeptide was recovered.

[0031] The present invention further relates to

[0032] (a) A polypeptide having at least 60% sequence identity with a mature polypeptide of SEQ ID NO: 2, 4 or 6; (b) A polypeptide encoded by a polynucleotide having at least 60% sequence identity with the mature polypeptide encoding sequence of SEQ ID NO: 1, 3 or 5; (c) Variants of the mature polypeptide of SEQ ID NO: 2, 4, or 6, wherein the variant contains substitution, deletion, and / or insertion at one or more positions; and Fragments of polypeptides (d), (a), (b), or (c) that have DNase activity.

[0033] In another aspect, the present invention relates to detergent compositions comprising a polypeptide having DNase activity and preferably a detergent adjuvant component. One aspect of the invention relates to a composition comprising a polypeptide having DNase activity and a detergent adjuvant, the polypeptide having at least 60% sequence identity with a mature polypeptide of SEQ ID NO: 2, 4, or 6.

[0034] The present invention further relates to a cleaning or washing method for cleaning or washing articles, the method comprising the following steps: a. Exposing articles to a detergent solution containing a polypeptide with DNase activity or to a detergent composition containing a polypeptide with DNase activity; b. Complete at least one washing cycle; and c. Optionally rinse the item. The article is a textile and the polypeptide with DNase activity is a polypeptide that has at least 60% sequence identity with the polypeptide of SEQ ID NO: 8, 9 or 10.

[0035] In addition, protection is sought for the use of DNases to prevent, reduce, or remove biofilms from articles.

[0036] The present invention further relates to nucleotides encoding these polypeptides and methods for generating these polypeptides.

[0037] sequence

[0038] SEQ ID NO 1: Obtained from a species of the genus Bacillus - 62451 DNA sequence

[0039] SEQ ID NO 2 is a polypeptide sequence derived from SEQ ID NO 1.

[0040] SEQ ID NO 3: DNA sequence obtained from Bacillus Horikoshii

[0041] SEQ ID NO 4: is a polypeptide sequence derived from SEQ ID NO 3.

[0042] SEQ ID NO 5: Obtained from a species of the genus Bacillus- 18057 DNA sequence

[0043] SEQ ID NO 6: is a polypeptide sequence derived from SEQ ID NO 3.

[0044] SEQ ID NO 7: Mature polypeptide Benzonase DNA enzyme (WO 2011 / 098579)

[0045] SEQ ID NO 8: Obtained from a species of the genus Bacillus - 62451 Mature polypeptide of SEQ ID NO 2

[0046] SEQ ID NO 9: Mature polypeptide obtained from Bacillus Horikoshii SEQ ID NO 4

[0047] SEQ ID NO 10: Obtained from a species of the genus Bacillus- 18057 Mature polypeptide of SEQ ID NO 6

[0048] SEQ ID NO 11: Obtained from a species of the genus Bacillus - 62520 Mature polypeptides

[0049] SEQ ID NO 12: Obtained from a species of the genus Bacillus- 62520 Mature polypeptides

[0050] SEQ ID NO 13: Mature polypeptide obtained from Bacillus Horikoshii

[0051] SEQ ID NO 14: Mature polypeptide obtained from Bacillus Horikoshii

[0052] SEQ ID NO 15: Obtained from a species of the genus Bacillus - 16840 Mature polypeptides

[0053] SEQ ID NO 16: Obtained from a species of the genus Bacillus - 16840 Mature polypeptides

[0054] SEQ ID NO 17: Obtained from a species of the genus Bacillus -62668 Mature polypeptides

[0055] SEQ ID NO 18: Obtained from a species of the genus Bacillus - 13395 Mature polypeptides

[0056] SEQ ID NO 19: Mature polypeptide obtained from Bacillus thuringiensis

[0057] SEQ ID NO 20: Obtained from a species of the genus Bacillus - 11238 Mature polypeptides

[0058] SEQ ID NO 21: Obtained from Bacillus foodae ( Bacillus cibi Mature polypeptides

[0059] SEQ ID NO 22: Obtained from a species of the genus Bacillus- 18318 Mature polypeptides

[0060] SEQ ID NO 23: Mature polypeptide obtained from Bacillus subtilis at the Institute of Pathogenic Biology

[0061] SEQ ID NO 24: is a secretion signal of Bacillus clausti.

[0062] SEQ ID NO 25: DNA sequence obtained from Bacillus species-62520

[0063] SEQ ID NO: 26: Peptide sequence derived from SEQ ID NO: 25

[0064] SEQ ID NO 27: DNA sequence obtained from Bacillus species-62520

[0065] SEQ ID NO: 28: Peptide sequence derived from SEQ ID NO: 27

[0066] SEQ ID NO 29: DNA sequence obtained from Bacillus Horikoshii

[0067] SEQ ID NO: 30: Polypeptide sequence derived from SEQ ID NO: 29

[0068] SEQ ID NO 31: DNA sequence obtained from Bacillus Horikoshii

[0069] SEQ ID NO: 32: Polypeptide sequence derived from SEQ ID NO: 31

[0070] SEQ ID NO 33: DNA sequence obtained from Bacillus species-16840

[0071] SEQ ID NO: 34: Polypeptide sequence derived from SEQ ID NO: 33

[0072] SEQ ID NO 35: DNA sequence obtained from Bacillus species-16840

[0073] SEQ ID NO: 36: Peptide sequence derived from SEQ ID NO: 35

[0074] SEQ ID NO 37: DNA sequence obtained from Bacillus species-62668

[0075] SEQ ID NO: 38: Peptide sequence derived from SEQ ID NO: 37

[0076] SEQ ID NO 39: DNA sequence obtained from Bacillus species-13395

[0077] SEQ ID NO: 40: Peptide sequence derived from SEQ ID NO: 39

[0078] SEQ ID NO 41: DNA sequence obtained from Bacillus horneri

[0079] SEQ ID NO: 42: Peptide sequence derived from SEQ ID NO: 41

[0080] SEQ ID NO 43: DNA sequence obtained from Bacillus species-11238

[0081] SEQ ID NO: 44: Polypeptide sequence derived from SEQ ID NO: 43

[0082] SEQ ID NO 45: DNA sequence obtained from food Bacillus.

[0083] SEQ ID NO: 46: Peptide sequence derived from SEQ ID NO: 45

[0084] SEQ ID NO 47: DNA sequence obtained from Bacillus species-18318

[0085] SEQ ID NO: 48: Peptide sequence derived from SEQ ID NO: 47

[0086] SEQ ID NO 49: DNA sequence obtained from Bacillus subtilis at the Institute of Pathogenic Biology

[0087] SEQ ID NO 50: Peptide sequence derived from SEQ ID NO: 49

[0088] SEQ ID NO 51: DNA sequence of Bacillus algicola obtained

[0089] SEQ ID NO: 52: Peptide sequence derived from SEQ ID NO: 51

[0090] SEQ ID NO 53 is a mature polypeptide obtained from Bacillus oryzae.

[0091] SEQ ID NO 54: DNA sequence derived from xanthan alkaline community J.

[0092] SEQ ID NO 55: Peptide sequence derived from SEQ ID NO: 54

[0093] SEQ ID NO 56: Mature polypeptide obtained from xanthan gum basic community J

[0094] SEQ ID NO 57: DNA sequence obtained from Bacillus vivax.

[0095] SEQ ID NO 58: Peptide sequence derived from SEQ ID NO: 57

[0096] SEQ ID NO 59: Mature polypeptide obtained from Bacillus vinifera

[0097] SEQ ID NO 60: DNA sequence obtained from Bacillus huajintan

[0098] SEQ ID NO: 61: Peptide sequence derived from SEQ ID NO: 60

[0099] SEQ ID NO 62: Mature polypeptide obtained from Bacillus huajintan

[0100] SEQ ID NO 63: DNA sequence obtained from Bacillus spp.

[0101] SEQ ID NO: 64: Polypeptide sequence derived from SEQ ID NO: 63

[0102] SEQ ID NO 65: Mature polypeptide obtained from Bacillus subtilis

[0103] SEQ ID NO 66: DNA sequence obtained from Bacillus indicus

[0104] SEQ ID NO: 67: Peptide sequence derived from SEQ ID NO: 66

[0105] SEQ ID NO 68: Mature polypeptide obtained from Bacillus indusia

[0106] SEQ ID NO 69: DNA sequence obtained from Bacillus chrysogenum.

[0107] SEQ ID NO 70: Peptide sequence derived from SEQ ID NO: 69

[0108] SEQ ID NO 71: Mature polypeptide obtained from Bacillus chrysogenum

[0109] SEQ ID NO 72: DNA sequence obtained from Bacillus luciferensis

[0110] SEQ ID NO: 73: Peptide sequence derived from SEQ ID NO: 72

[0111] SEQ ID NO 74: Mature polypeptide obtained from Bacillus Lucifer

[0112] SEQ ID NO 75: DNA sequence obtained from Bacillus chrysogenum.

[0113] SEQ ID NO: 76: Peptide sequence derived from SEQ ID NO: 75

[0114] SEQ ID NO 77: Mature polypeptide obtained from Bacillus chrysogenum

[0115] SEQ ID NO 78: DNA sequence obtained from Bacillus species SA2-6

[0116] SEQ ID NO 79: Peptide sequence derived from SEQ ID NO: 78

[0117] SEQ ID NO 80: Mature polypeptide obtained from Bacillus species SA2-6

[0118] SEQ ID NO 81: DNA sequence obtained from a species of the genus *Pyrenochaetopsis*

[0119] SEQ ID NO: 82: Peptide sequence derived from SEQ ID NO: 81

[0120] SEQ ID NO 83: Mature polypeptide obtained from a species of the genus *Echinochloa*

[0121] SEQ ID NO 84: DNA sequence obtained from Vibrissea flavovirens

[0122] SEQ ID NO: 85: Peptide sequence derived from SEQ ID NO: 84

[0123] SEQ ID NO 86: Mature peptide obtained from Vibrissea flavovirens

[0124] SEQ ID NO 87: DNA sequence obtained from Setosphaeria rostrate

[0125] SEQ ID NO: 88: Peptide sequence derived from SEQ ID NO: 87

[0126] SEQ ID NO 89: Mature polypeptide obtained from Setosphaeria rostrate

[0127] SEQ ID NO 90: DNA sequence obtained from Endophragmiella valdina

[0128] SEQ ID NO: 91: Peptide sequence derived from SEQ ID NO: 90

[0129] SEQ ID NO 92: Mature polypeptide obtained from Endophragmiella valdina

[0130] SEQ ID NO 93: DNA sequence obtained from *Corynebacterium multiflorum*

[0131] SEQ ID NO: 94: Polypeptide sequence derived from SEQ ID NO: 93

[0132] SEQ ID NO 95: Mature polypeptide obtained from *Corynebacterium multiflorum*

[0133] SEQ ID NO 96: DNA sequence obtained from species XZ1965 of the genus *Heterostylus*.

[0134] SEQ ID NO 97: Peptide sequence derived from SEQ ID NO: 96

[0135] SEQ ID NO 98: Mature polypeptide obtained from species XZ1965 of the genus *Heterostylium*.

[0136] SEQ ID NO 99: DNA sequence obtained from the fungus causing peach brown rot.

[0137] SEQ ID NO 100: Peptide sequence derived from SEQ ID NO: 99

[0138] SEQ ID NO 101: Mature polypeptide obtained from *Prunus cerevisiae*.

[0139] SEQ ID NO 102: DNA sequence obtained from Curvularia crescentis

[0140] SEQ ID NO: 103: Polypeptide sequence derived from SEQ ID NO: 102

[0141] SEQ ID NO 104: Mature polypeptide obtained from Curvularia crescentis

[0142] SEQ ID NO 105: DNA sequence obtained from Penicillium spp.

[0143] SEQ ID NO: 106: Peptide sequence derived from SEQ ID NO: 105

[0144] SEQ ID NO 107: Mature polypeptide obtained from Penicillium spp.

[0145] SEQ ID NO 108: DNA sequence obtained from Penicillium quercetorum

[0146] SEQ ID NO: 109: Peptide sequence derived from SEQ ID NO: 108

[0147] SEQ ID NO 110: Mature polypeptide obtained from Penicillium quercetorum

[0148] SEQ ID NO 111: DNA sequence obtained from a species of the genus Setophaeosphaeria

[0149] SEQ ID NO: 112: Peptide sequence derived from SEQ ID NO: 111

[0150] SEQ ID NO 113: Mature polypeptide obtained from species of the genus Setophaeosphaeria

[0151] SEQ ID NO 114: DNA sequence obtained from Alternaria species XZ2545

[0152] SEQ ID NO: 115: Peptide sequence derived from SEQ ID NO: 114

[0153] SEQ ID NO 116: Mature polypeptide obtained from Alternaria species XZ2545

[0154] SEQ ID NO 117: DNA sequence obtained from a species of Alternaria.

[0155] SEQ ID NO: 118: Peptide sequence derived from SEQ ID NO: 117

[0156] SEQ ID NO 119: Mature polypeptide obtained from Alternaria species

[0157] SEQ ID NO 120: DNA sequence obtained from Trichoderma reesei

[0158] SEQ ID NO: 121: Peptide sequence derived from SEQ ID NO: 121

[0159] SEQ ID NO 122: Mature polypeptide obtained from Trichoderma reesei

[0160] SEQ ID NO 123: DNA sequence obtained from *Chaetoceros thermophilus*

[0161] SEQ ID NO: 124: Polypeptide sequence derived from SEQ ID NO: 123

[0162] SEQ ID NO 125: Mature polypeptide obtained from *Chaetoceros thermophilus*

[0163] SEQ ID NO 126: DNA sequence obtained from *Lysimachia thermophila*

[0164] SEQ ID NO: 127: Peptide sequence derived from SEQ ID NO: 126

[0165] SEQ ID NO 128: Mature polypeptide obtained from *Lysimachia thermophila*

[0166] SEQ ID NO 129: DNA sequence obtained from Metapochonia suchlasporia

[0167] SEQ ID NO 130: Polypeptide sequence derived from SEQ ID NO: 129

[0168] SEQ ID NO 131: Mature polypeptide obtained from Metapochonia suchlasporia

[0169] SEQ ID NO 132: DNA sequence obtained from *Carya spp.*

[0170] SEQ ID NO: 133: Polypeptide sequence derived from SEQ ID NO: 132

[0171] SEQ ID NO 134: Obtaining mature polypeptides from *Carya spp.*

[0172] SEQ ID NO 135: DNA sequence obtained from species XZ2007 of the genus *Cladosporium*.

[0173] SEQ ID NO: 136: Peptide sequence derived from SEQ ID NO: 135

[0174] SEQ ID NO 137: Mature polypeptide obtained from species XZ2007 of the genus *Cladosporium*

[0175] SEQ ID NO 138: DNA sequence obtained from Acremonium dichromosporum

[0176] SEQ ID NO: 139: Peptide sequence derived from SEQ ID NO: 138

[0177] SEQ ID NO 140: Mature polypeptide obtained from *Acer dichromatosa*

[0178] SEQ ID NO 141: DNA sequence obtained from species XZ2014 of the genus *Pleurotus*.

[0179] SEQ ID NO: 142: Polypeptide sequence derived from SEQ ID NO: 141

[0180] SEQ ID NO 143: Mature polypeptide obtained from species XZ2014 of the genus *Cladosporium*

[0181] SEQ ID NO 144: DNA sequence obtained from species HNA15-2 of the genus Metarhizium.

[0182] SEQ ID NO 145: Peptide sequence derived from SEQ ID NO: 144

[0183] SEQ ID NO 146: Mature polypeptide obtained from Metarhizium species HNA15-2

[0184] SEQ ID NO 147: DNA sequence obtained from species XZ2414 of the genus *Cladosporium*.

[0185] SEQ ID NO 148: Polypeptide sequence derived from SEQ ID NO: 147

[0186] SEQ ID NO 149: Mature polypeptide obtained from species XZ2414 of the genus *Cladosporium*

[0187] SEQ ID NO 150: DNA sequence obtained from *Bracteolesia lataniae*

[0188] SEQ ID NO: 151: Peptide sequence derived from SEQ ID NO: 150

[0189] SEQ ID NO 152: Mature polypeptide obtained from *Bractea spp.*

[0190] SEQ ID NO 153: DNA sequence obtained from Scytalidium circinatum

[0191] SEQ ID NO: 154: Peptide sequence derived from SEQ ID NO: 153

[0192] SEQ ID NO 155: Mature polypeptide obtained from *Hylocereus spiralis*

[0193] SEQ ID NO 156: DNA sequence obtained from Metarhizium lepidiotae

[0194] SEQ ID NO: 157: Peptide sequence derived from SEQ ID NO: 156

[0195] SEQ ID NO 158: Mature polypeptide obtained from Metarhizium anisopliae

[0196] SEQ ID NO 159: DNA sequence obtained from *Thermobispora bispora*

[0197] SEQ ID NO 160: Peptide sequence derived from SEQ ID NO: 159

[0198] SEQ ID NO 161: Mature polypeptide obtained from *Amycium bisporum*

[0199] SEQ ID NO 162: DNA sequence obtained from Spormia fimetaria

[0200] SEQ ID NO: 163: Peptide sequence derived from SEQ ID NO: 162

[0201] SEQ ID NO 164: Mature polypeptide obtained from Spormia fimetaria

[0202] SEQ ID NO 165: DNA sequence obtained from Pycnidiophora cf. dispera

[0203] SEQ ID NO: 166: Peptide sequence derived from SEQ ID NO: 165

[0204] SEQ ID NO 167: Mature polypeptide obtained from Pycnidiophora cf. dispera

[0205] SEQ ID NO 168: DNA sequence obtained from xanthan gum basic community D

[0206] SEQ ID NO: 169: Peptide sequence derived from SEQ ID NO: 168

[0207] SEQ ID NO 170: Mature polypeptide obtained from xanthan gum basic community D

[0208] SEQ ID NO 171: DNA sequence obtained from xanthan gum basic community O

[0209] SEQ ID NO: 172: Peptide sequence derived from SEQ ID NO: 171

[0210] SEQ ID NO 173: Mature polypeptide obtained from xanthan gum basic community O

[0211] SEQ ID NO 174: DNA sequence obtained from species-70249 of the Clavicipitaceae family.

[0212] SEQ ID NO: 175: Peptide sequence derived from SEQ ID NO: 174

[0213] SEQ ID NO 176: Mature polypeptide 175 obtained from species-70249 of the Clavicipitaceae family.

[0214] SEQ ID NO 177: DNA sequence obtained from species AS85-2 of the genus Westerdykella.

[0215] SEQ ID NO 178: Peptide sequence derived from SEQ ID NO: 177

[0216] SEQ ID NO 179: Mature polypeptide obtained from species AS85-2 of the genus *Westernia*.

[0217] SEQ ID NO 180: DNA sequence obtained from Humicolopsis cephalosporioides

[0218] SEQ ID NO 181: Peptide sequence derived from SEQ ID NO: 180

[0219] SEQ ID NO 182: Mature polypeptide obtained from Humicolopsis cephalosporioides

[0220] SEQ ID NO 183: DNA sequence obtained from Neosartorya massa

[0221] SEQ ID NO: 184: Peptide sequence derived from SEQ ID NO: 183

[0222] SEQ ID NO 185: Mature polypeptide obtained from Neosartorya massa

[0223] SEQ ID NO 186: DNA sequence obtained from Roussoella intermedia

[0224] SEQ ID NO 187: Peptide sequence derived from SEQ ID NO: 186

[0225] SEQ ID NO 188: Mature polypeptide obtained from SEQ ID NO 187

[0226] SEQ ID NO 189: DNA sequence obtained from the target cavity of the spore.

[0227] SEQ ID NO 190: Peptide sequence derived from SEQ ID NO: 189

[0228] SEQ ID NO 191: Obtaining mature polypeptide from spore cavity

[0229] SEQ ID NO 192: DNA sequence obtained from the genus *Cyclocarya*.

[0230] SEQ ID NO: 193: Polypeptide sequence derived from SEQ ID NO: 192

[0231] SEQ ID NO 194: Mature polypeptide obtained from *Cyclocarya* spp.

[0232] SEQ ID NO 195: DNA sequence obtained from Didymosphaeria futilis

[0233] SEQ ID NO: 196: Peptide sequence derived from SEQ ID NO: 195

[0234] SEQ ID NO 197: Mature polypeptide obtained from Didymosphaeria futilis

[0235] SEQ ID NO 198: Motif [T / D / S][G / N]PQL

[0236] SEQ ID NO 199: Motif [G / T]Y[D / S][R / K / L]

[0237] SEQ ID NO 200: Motif [E / D / H]H[I / V / L / F / M]X[P / A / S]

[0238] SEQ ID NO 201: Motif [F / L / Y / I]A[N / R]D[L / I / P / V]

[0239] SEQ ID NO 202: Motif C[D / N]T[A / R]

[0240] SEQ ID NO 203: Motif [D / Q][I / V]DH

[0241] SEQ ID NO 204: Motif [D / M / L][S / T]GYSR[D / N]

[0242] SEQ ID NO 205: motif ASXNRSKG

[0243] SEQ ID NO 206: Motif [V / I]PL[S / A]NAWK

[0244] SEQ ID NO 207: Motif NPQL

[0245] SEQ ID NO 208: Motif P[Q / E]L[W / Y]

[0246] SEQ ID NO 209: Motif [K / H / E]NAW

[0247] definition

[0248] Allele variants: The term "allelic variant" refers to any of two or more alternative forms of a gene occupying the same chromosomal locus. Allelic variations arise naturally through mutation and can lead to polymorphism within a population. Gene mutations can be silent (without alteration in the encoded polypeptide) or can encode a polypeptide with a modified amino acid sequence. An allelic variant of a polypeptide is a polypeptide encoded by an allelic variant of a gene.

[0249] biofilm Biofilms are groups of microorganisms whose cells adhere to each other on surfaces such as textiles, tableware, or hard surfaces. These adherent cells are often embedded within a matrix of extracellular polymers (EPS). Biofilms (EPS) are polymeric aggregates generally composed of extracellular DNA, proteins, and polysaccharides. Biofilms can form on living or non-living surfaces. Microbial cells growing in biofilms are physiologically different from planktonic cells of the same organism (in contrast to single cells that can float or float in liquid culture media). Bacteria living in biofilms often possess significantly different characteristics from freely floating bacteria of the same species because the dense and protected environment of the membrane allows them to cooperate and interact in different ways. One benefit of this environment is increased resistance to detergents and antibiotics, as the dense extracellular matrix and outer layer of the cells protect the interior of the community. On clothing, bacteria that produce biofilms can be found in the following species: Acinetobacter species, Aerobicmycium species, Shortwave Monoclonal species, Microbacterium species, Micrococcus flavonoides, Pseudomonas species, Staphylococcus epidermidis, and Oligotrophozoites species.

[0250] Encoded sequence: The term "coding sequence" refers to a polynucleotide that directly specifies the amino acid sequence of a polypeptide. The boundaries of a coding sequence are generally determined by an open reading frame (ORF), which begins with a start codon (such as ATG, GTG, or TTG) and ends with a stop codon (such as TAA, TAG, or TGA). Coding sequences can be genomic DNA, synthetic DNA, or a combination thereof.

[0251] Color difference (L value): The Lab color space is a color space that addresses the contrast between lightness and darkness, with a value represented by the dimension L. The L value represents the contrast between lightness and darkness. Representative in L = 0, the darkest black, and for L = 100 is the brightest white. In the context of this invention, the L value is also referred to as color difference.

[0252] control sequenceThe term "control sequence" refers to the nucleic acid sequence necessary for the expression of a polynucleotide encoding the mature polypeptide of the present invention. Each control sequence may be native (i.e., from the same gene) or exogenous (i.e., from a different gene) for the polynucleotide encoding the polypeptide, or native or exogenous relative to each other. Such control sequences include, but are not limited to, precursors, polyadenylated sequences, propeptide sequences, promoters, signal peptide sequences, and transcription terminators. At a minimum, control sequences include promoters and transcription and translation termination signals. These control sequences may be provided with multiple linkers for the purpose of introducing specific restriction enzyme sites that facilitate the linking of these control sequences to the coding regions of the polynucleotide encoding the polypeptide.

[0253] Deep cleaning: The term "deep cleaning" refers to the disruption or removal of biofilms or components of biofilms, such as polysaccharides, proteins, DNA, dirt, or other components present in biofilms.

[0254] Detergent adjuvant ingredients: The detergent adjuvant components differ from the DNA enzymes of this invention. The precise properties of these additional adjuvant components, and their incorporation levels, will depend on the physical form of the composition and the nature of the operation in which the composition will be used. Suitable adjuvant materials include, but are not limited to, the components described below, such as surfactants, detergent builders, flocculants, chelating agents, dye transfer inhibitors, enzymes, enzyme stabilizers, enzyme inhibitors, catalytic materials, bleaching activators, hydrogen peroxide, hydrogen peroxide sources, pre-formed peracids, polymerized dispersants, clay detergents / anti-reprecipitants, brighteners, antifoaming agents, dyes, fragrances, structural elastic agents, fabric softeners, carriers, water-soluble additives, detergent builders and co-detergent builders, fabric colorants, antifoaming agents, dispersants, processing aids, and / or pigments.

[0255] Detergent compositionThe term "detergent composition" refers to a composition used to remove unwanted compounds from items to be cleaned, such as textiles. This detergent composition can be used, for example, to clean textiles, for both household and industrial cleaning. These terms encompass any material / compound selected for the specific type of cleaning composition and product desired (e.g., liquid, gel, powder, granules, paste, or spray compositions), and include, but are not limited to, detergent compositions (e.g., liquid and / or solid laundry detergents and delicate fabric detergents; fabric fresheners; fabric softeners; and textile and garment pre-treatments / pre-treatments). In addition to the enzymes of this invention, the detergent formulation may also contain one or more other enzymes (such as proteases, amylases, lipases, keratases, cellulases, endoglucanases, xyloglucanases, pectins, pectin lyases, xanthan gums, peroxidases, halogenated peroxidases, catalases, and mannanases, or any mixture thereof), and / or detergent adjuvants such as surfactants, builders, chelators or chelating agents, bleaching systems or bleaching components, polymers, fabric conditioners, foaming agents, defoaming agents, dyes, fragrances, darkening inhibitors, optical brighteners, bactericides, fungicides, dirt suspending agents, corrosion inhibitors, enzyme inhibitors or stabilizers, enzyme activators, an EPA transferase, hydrolases, oxidoreductases, bluing agents and fluorescent dyes, antioxidants, and solubilizers.

[0256] DNA enzyme (deoxyribonuclease) The term "DNase" refers to a polypeptide with DNase activity that catalyzes the hydrolytic breakage of phosphodiester bonds in the DNA backbone, thereby degrading DNA. The terms "DNase" and the expression "polypeptide with DNase activity" are used interchangeably throughout this application. For the purposes of this invention, DNase activity is determined according to the procedure described in Assay I. In one aspect, the polypeptide of this invention has at least 20%, for example at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or at least 100% of the DNase activity of a mature polypeptide of SEQ ID NO: 2, 4, or 6 (preferably SEQ ID NO: 2). In one embodiment, the polypeptide of the present invention has improved DNase activity, for example, such that the DNase activity of the polypeptide is at least 105% of the DNase activity of the mature polypeptide of SEQ ID NO: 2, 4, or 6 (preferably SEQ ID NO: 2), for example, at least 110%, at least 120%, at least 130%, at least 140%, at least 160%, at least 170%, at least 180%, or at least 200%.

[0257] In a preferred embodiment, the DNA enzyme activity of the polypeptide is at least 20% of the DNA enzyme activity of the mature polypeptide of SEQ ID NO: 2 as determined according to the procedure described in Assay I, for example at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 100%, at least 105%, at least 110%, at least 120%, at least 130%, at least 140%, at least 160%, at least 170%, at least 180%, or at least 200%.

[0258] Benefits of enzyme washing The term "enzyme washing benefit" is defined here as the advantageous effect of adding an enzyme to a detergent compared to the same detergent without that enzyme. Important washing benefits that enzymes may provide include stain removal with little or no visible dirt after washing and / or cleaning, prevention or reduction of redeposition of dirt released during washing (also known as anti-redeposition effect), and complete or partial restoration of the whiteness of textiles that were originally white but have acquired a pale gray or yellowish appearance after repeated use and washing. Textile care benefits not directly related to the catalytic removal of stains or the prevention of their redeposition are also important to the enzyme washing benefit. Examples of such textile care benefits include preventing or reducing dye transfer from one fabric to another or another part of the same fabric (also known as dye transfer inhibition or anti-reversion effect), removing protruding or broken fibers from the fabric surface to reduce pilling tendency or removing existing fuzz or fluff (also known as anti-pilling effect), improving fabric softness, clarifying fabric colors, and removing particulate dirt trapped in the fibers of fabrics or garments. Enzymatic bleaching is another benefit of enzymatic washing, where catalytic activity is typically used to catalyze the formation of bleaching components such as hydrogen peroxide or other peroxides.

[0259] Express: The term “expression” includes any step involved in polypeptide production, including but not limited to transcription, post-transcriptional modification, translation, post-translational modification, and secretion.

[0260] Vehicle for expression: The term "expression vector" refers to a linear or circular DNA molecule that contains a polynucleotide encoding a polypeptide and that the polynucleotide is operatively linked to a control sequence for its expression.

[0261] Excerpt:The term "fragment" refers to a polypeptide that has one or more (e.g., several) amino acids deleted from the amino and / or carboxyl ends of a mature polypeptide or domain; wherein the fragment has DNase activity. In one aspect, the fragment comprises at least 206 amino acid residues (e.g., amino acids 1 to 206 of SEQ ID NO: 2), at least 205 amino acid residues (e.g., amino acids 2 to 206 of SEQ ID NO: 2), or at least 204 amino acid residues (e.g., amino acids 3 to 206 of SEQ ID NO: 2). In another aspect, the fragment comprises at least 206 amino acid residues (e.g., amino acids 1 to 206 of SEQ ID NO: 4), at least 205 amino acid residues (e.g., amino acids 4 to 206 of SEQ ID NO: 4), or at least 204 amino acid residues (e.g., amino acids 3 to 206 of SEQ ID NO: 4). In one aspect, the fragment contains at least 206 amino acid residues (e.g., amino acids 1 to 206 of SEQ ID NO: 6), at least 205 amino acid residues (e.g., amino acids 2 to 206 of SEQ ID NO: 6), or at least 204 amino acid residues (e.g., amino acids 3 to 206 of SEQ ID NO: 6).

[0262] Host cell: The term "host cell" refers to any cell type that is readily transformed, transfected, transduced, etc., using nucleic acid constructs or expression vectors containing the polynucleotides of the present invention. The term "host cell" also encompasses any offspring of the parent cell that differs from the parent cell due to mutations occurring during replication.

[0263] Improved washing performance The term "improved washing performance" is defined herein as the washing performance of an enzyme in a detergent composition that exhibits increased washing performance relative to the same detergent composition without the enzyme, for example, by increasing stain removal or reducing redeposition. The term "improved washing performance" includes washing performance in clothing.

[0264] Separate:The term "isolated" means a substance in a form or environment not naturally occurring. Non-limiting examples of isolated substances include (1) any substance not naturally occurring; (2) any substance, including but not limited to any enzyme, variant, nucleic acid, protein, peptide, or cofactor, which is at least partially removed from one or more of the naturally occurring components associated with it; (3) any substance artificially modified relative to a substance found in nature; or (4) any substance modified by increasing the amount of the substance relative to other components naturally associated with it (e.g., recombinant generation in a host cell; multiple copies of the gene encoding the substance; and the use of a promoter stronger than that naturally associated with the gene encoding the substance). Isolated substances may be present in fermentation broth samples; for example, host cells may be genetically modified to express the polypeptides of the invention. Fermentation broth from such host cells will contain the isolated polypeptides.

[0265] Laundry: The term "laundry" encompasses both household and industrial laundry and refers to the process of treating textiles with a solution containing the cleaning or detergent composition of the present invention. The laundry process can be performed, for example, using a household or industrial washing machine or manually.

[0266] The term "foul odor" refers to an undesirable smell on a cleaned item. A cleaned item should smell fresh and clean, without any foul odor adhering to it. An example of foul odor is a compound with an unpleasant smell, which can be produced by microorganisms. Another example is the smell of sweat or body odor that can adhere to an item that has come into contact with humans or animals. Yet another example of foul odor can be the smell of spices that adhere to an item, such as strongly scented curry or other exotic spices. One way to measure the ability of an item to adhere to foul odor is by using the determination II disclosed herein.

[0267] Mature polypeptides: The term "mature polypeptide" refers to a polypeptide in its final form after translation and any post-translational modifications such as N-terminal processing, C-terminal truncation, glycosylation, phosphorylation, etc. In one aspect, a mature polypeptide is amino acids 29 to 210 of SEQ ID NO: 2, amino acids 29 to 210 of SEQ ID NO: 4, or amino acids 23 to 202 of SEQ ID NO: 6, and amino acids 1 to 28 of SEQ ID NO: 2, amino acids 1 to 28 of SEQ ID NO: 4, and amino acids 1 to 22 of SEQ ID NO: 6 are signal peptides.

[0268] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 26.

[0269] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 28.

[0270] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 30.

[0271] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 32.

[0272] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 34.

[0273] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 36.

[0274] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 38.

[0275] On one hand, the mature polypeptide is amino acids 1 to 183 of SEQ ID NO: 40.

[0276] On one hand, the mature polypeptide is amino acids 1 to 185 of SEQ ID NO: 42.

[0277] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 44.

[0278] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 46.

[0279] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 48.

[0280] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 50.

[0281] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 52.

[0282] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 55.

[0283] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 58.

[0284] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 61.

[0285] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 64.

[0286] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 67.

[0287] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 70.

[0288] On one hand, the mature polypeptide is amino acids 1 to 184 of SEQ ID NO: 73.

[0289] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 76.

[0290] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 79.

[0291] On one hand, the mature polypeptide is amino acids 1 to 191 of SEQ ID NO: 82.

[0292] On one hand, the mature polypeptide is amino acids 1 to 190 of SEQ ID NO: 85.

[0293] On one hand, the mature polypeptide is amino acids 1 to 192 of SEQ ID NO: 88.

[0294] On one hand, the mature polypeptide is amino acids 1 to 192 of SEQ ID NO: 91.

[0295] On one hand, the mature polypeptide is amino acids 1 to 190 of SEQ ID NO: 94.

[0296] On one hand, the mature polypeptide is amino acids 1 to 192 of SEQ ID NO: 97.

[0297] On one hand, the mature polypeptide is amino acids 1 to 186 of SEQ ID NO: 100.

[0298] On one hand, the mature polypeptide is amino acids 1 to 190 of SEQ ID NO: 103.

[0299] On one hand, the mature polypeptide is amino acids 1 to 191 of SEQ ID NO: 106.

[0300] On one hand, the mature polypeptide is amino acids 1 to 191 of SEQ ID NO: 109.

[0301] On one hand, the mature polypeptide is amino acids 1 to 192 of SEQ ID NO: 112.

[0302] On one hand, the mature polypeptide is amino acids 1 to 192 of SEQ ID NO: 115.

[0303] On one hand, the mature polypeptide is amino acids 1 to 192 of SEQ ID NO: 118.

[0304] On one hand, the mature polypeptide is amino acids 1 to 186 of SEQ ID NO: 121.

[0305] On one hand, the mature polypeptide is amino acids 1 to 188 of SEQ ID NO: 124.

[0306] On one hand, the mature polypeptide is amino acids 1 to 190 of SEQ ID NO: 127.

[0307] On one hand, the mature polypeptide is amino acids 1 to 186 of SEQ ID NO: 130.

[0308] On one hand, the mature polypeptide is amino acids 1 to 198 of SEQ ID NO: 133.

[0309] On one hand, the mature polypeptide is amino acids 1 to 188 of SEQ ID NO: 136.

[0310] On one hand, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO: 139.

[0311] On one hand, the mature polypeptide is amino acids 1 to 188 of SEQ ID NO: 142.

[0312] On one hand, the mature polypeptide is amino acids 1 to 186 of SEQ ID NO: 145.

[0313] On one hand, the mature polypeptide is amino acids 1 to 188 of SEQ ID NO: 148.

[0314] On one hand, the mature polypeptide is amino acids 1 to 186 of SEQ ID NO: 151.

[0315] On one hand, the mature polypeptide is amino acids 1 to 184 of SEQ ID NO: 154.

[0316] On one hand, the mature polypeptide is amino acids 1 to 186 of SEQ ID NO: 157.

[0317] On one hand, the mature polypeptide is amino acids 1 to 226 of SEQ ID NO: 160.

[0318] On one hand, the mature polypeptide is amino acids 1 to 191 of SEQ ID NO: 163.

[0319] On one hand, the mature polypeptide is amino acids 1 to 193 of SEQ ID NO: 166.

[0320] On one hand, the mature polypeptide is amino acids 1 to 199 of SEQ ID NO: 169.

[0321] On one hand, the mature polypeptide is amino acids 1 to 194 of SEQ ID NO: 172.

[0322] On one hand, the mature polypeptide is amino acids 1 to 186 of SEQ ID NO: 175.

[0323] On one hand, the mature polypeptide is amino acids 1 to 187 of SEQ ID NO: 178.

[0324] On one hand, the mature polypeptide is amino acids 1 to 194 of SEQ ID NO: 181.

[0325] On one hand, the mature polypeptide is amino acids 1 to 190 of SEQ ID NO: 184.

[0326] On one hand, the mature polypeptide is amino acids 1 to 191 of SEQ ID NO: 187.

[0327] On one hand, the mature polypeptide is amino acids 1 to 191 of SEQ ID NO: 190.

[0328] On one hand, the mature polypeptide is amino acids 1 to 192 of SEQ ID NO: 193.

[0329] On one hand, the mature polypeptide is amino acids 1 to 189 of SEQ ID NO: 196.

[0330] It is known in the art that host cells can produce mixtures of two or more different mature polypeptides (i.e., with different C-terminal and / or N-terminal amino acids) expressed from the same polynucleotide. It is also known in the art that different host cells process polypeptides differently, and therefore a host cell expressing a polynucleotide may produce different mature polypeptides (e.g., with different C-terminal and / or N-terminal amino acids) compared to another host cell expressing the same polynucleotide. The mature polypeptide of SEQ ID NO 2 is SEQ ID NO 8, the mature polypeptide of SEQ ID NO 4 is SEQ ID NO 9, and the mature polypeptide of SEQ ID NO 6 is SEQ ID NO 10.

[0331] Mature polypeptide coding sequenceThe term "mature polypeptide coding sequence" refers to a polynucleotide encoding a mature polypeptide with DNase activity. In one aspect, the mature polypeptide coding sequence is nucleotides 85 to 630 of SEQ ID NO: 1, nucleotides 85 to 630 of SEQ ID NO: 3, and nucleotides 67 to 606 of SEQ ID NO: 5.

[0332] Nucleic acid constructs The term "nucleic acid construct" refers to a single-stranded or double-stranded nucleic acid molecule that is isolated from a naturally occurring gene, modified to contain nucleic acid segments in a manner not normally found in nature, or synthesized, and that contains one or more control sequences.

[0333] Operable connection The term "operably linked" refers to a configuration in which a control sequence is positioned relative to the coding sequence of a polynucleotide so that the control sequence guides the expression of the coding sequence.

[0334] Drug adjuvant ingredients This refers to any pharmaceutical excipient suitable for formulating pharmaceutical compounds.

[0335] Such excipients, carriers, delivery systems, etc., are well known to those skilled in the art and are described in textbooks such as the Remington Pharmaceutical Compendium, Mack Publishing, Easton, Pennsylvania, 1985.

[0336] Pharmaceutically acceptable excipients suitable for use in tablet formulations include, for example, inert diluents such as calcium carbonate, sodium carbonate, lactose, calcium phosphate, or sodium phosphate; granulating or disintegrants such as corn starch or alginate; binders such as starch, gelatin, or gum arabic; and lubricants such as magnesium stearate, stearic acid, or talc. Tablets may be uncoated, or they may be coated using known techniques to delay disintegration and absorption in the gastrointestinal tract, thereby providing a prolonged effect over a longer period. For example, delaying materials such as glyceryl monostearate or glyceryl distearate may be used.

[0337] For hard capsule formulations, the active ingredient can be mixed with an inert solid diluent such as calcium carbonate, calcium phosphate, or kaolin. For soft capsule formulations, the active ingredient can be mixed with an aqueous or oil-based medium, such as peanut oil, liquid paraffin, or olive oil.

[0338] Excipients suitable for manufacturing aqueous suspensions include suspending agents, such as sodium carboxymethyl cellulose, methyl cellulose, hydroxypropyl methyl cellulose, sodium alginate, polyvinylpyrrolidone, tragacanth gum, and gum arabic; dispersants or wetting agents may be natural phospholipids, such as lecithin, or condensation products of olefin oxides and fatty acids, such as polyoxyethylene stearate, or condensation products of ethylene oxide and long-chain aliphatic alcohols, such as heptadecetylated cetyl alcohol, or condensation products of ethylene oxide and esters obtained from fatty acids and hexitols, such as polyoxyethylene sorbitan monooleate, or condensation products of ethylene oxide and esters obtained from dehydrated fatty acids and hexitols, such as polyvinyl dehydrated sorbitan monooleate.

[0339] Aqueous suspensions may also contain one or more preservatives, such as benzoates, such as ethylparaben or n-propylparaben; one or more colorants; one or more flavorings; and one or more sweeteners, such as sucrose or saccharin.

[0340] Oil suspensions can be formulated by suspending the active ingredient in a vegetable oil, such as peanut oil, olive oil, sesame oil, or coconut oil, or in a mineral oil, such as liquid paraffin. The oil suspension may contain a thickener, such as beeswax, hard paraffin, or hexadecyl alcohol. Sweeteners and flavorings may be added. These compositions can be preserved by adding antioxidants, such as ascorbic acid and vitamin C.

[0341] Reflectance Washability is expressed as the reflectance value of a small piece of stained fabric. After washing and rinsing, the small pieces of fabric were laid flat and allowed to air dry overnight at room temperature. All washed small pieces of fabric were evaluated the day after washing. The reflectance of the small pieces of fabric was evaluated using a Macbeth Color Eye 7000 reflectance spectrophotometer with a very small aperture. Measurements were taken in the absence of UV in the incident light, and the reflectance at 460 nm was extracted.

[0342] Sequence consistency: The correlation between two amino acid sequences or two nucleotide sequences is described by the parameter “sequence consistency”. For the purposes of this invention, the Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, J. Mol. Biol) implemented in the Needle program of the EMBOSS package (The European Molecular Biology Open Software Suite, Rice et al., 2000, Trends Genet. 16: 276-277) (preferably version 5.0.0 or later) is used. .[Journal of Molecular Biology] 48: 443-453) was used to determine sequence identity between two amino acid sequences. The parameters used were a 10-fold nick opening penalty, a 0.5-fold nick expansion penalty, and an EBLOSUM62 (EMBOSS version of BLOSUM62) substitution matrix. The Needle output marked "Longest Identity" (obtained using the -non-simplification option) was used as the percentage identity and calculated as follows: (identical residues x 100) / (alignment length - total number of vacancies in the alignment). For the purposes of this invention, the Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, ibid.) was used to determine sequence consistency between two deoxyribonucleotide sequences, as implemented in the Needle program within the EMBOSS package (EMBOSS: The European Molecular Biology Open Software Suite, Rice et al., 2000, ibid.), preferably version 5.0.0 or later. The parameters used were a vacancy opening penalty of 10, a vacancy extension penalty of 0.5, and an EDNAFULL (EMBOSS version of NCBINUC 4.4) substitution matrix. The Needle output labeled “Longest Consistency” (obtained using the -non-simplification option) was used as the percentage consistency and calculated as follows: (identical deoxyribonucleotides × 100) / (length of alignment - total number of vacancies in alignment).

[0343] Strict conditions: the term" Very low stringency conditions This means that for probes with a length of at least 100 nucleotides, a standard DNA blotting procedure is followed, involving pre-hybridization and hybridization at 42°C in 5X SSPE, 0.3% SDS, 200 μg / ml of cleaved and denatured salmon sperm DNA, and 25% formamide for 12 to 24 hours. Finally, the vector material is washed three times at 45°C with 2X SSC and 0.2% SDS for 15 minutes each time.

[0344] the term" Low stringency conditions This means that for probes at least 100 nucleotides in length, a standard DNA blotting procedure is followed, involving pre-hybridization and hybridization at 42°C in 5X SSPE, 0.3% SDS, 200 μg / ml of cleaved and denatured salmon sperm DNA, and 25% formamide for 12 to 24 hours. Finally, the vector material is washed three times at 50°C with 2X SSC and 0.2% SDS for 15 minutes each time.

[0345] the term" Strict conditions"This refers to the standard DNA blotting procedure for probes at least 100 nucleotides in length, following a pre-hybridization and hybridization process at 42°C in 5X SSPE, 0.3% SDS, 200 μg / ml of cleaved and denatured salmon sperm DNA, and 35% formamide for 12 to 24 hours. Finally, the vector material is washed three times at 55°C with 2X SSC and 0.2% SDS for 15 minutes each time."

[0346] the term" Medium to high stringent conditions This means that for probes at least 100 nucleotides in length, a standard DNA blotting procedure is followed, involving pre-hybridization and hybridization at 42°C in 5X SSPE, 0.3% SDS, 200 μg / ml of cleaved and denatured salmon sperm DNA, and 35% formamide for 12 to 24 hours. Finally, the vector material is washed three times at 60°C with 2X SSC and 0.2% SDS for 15 minutes each time.

[0347] The term "highly stringent conditions" refers to pre-hybridization and hybridization at 42°C for 12 to 24 hours using standard DNA blotting procedures for probes at least 100 nucleotides in length, in 5X SSPE, 0.3% SDS, 200 μg / ml cleaved and denatured salmon sperm DNA, and 50% formamide. Finally, the vector material is washed three times at 65°C with 2X SSC and 0.2% SDS for 15 minutes each time.

[0348] the term" Very high stringent conditions This means that for probes at least 100 nucleotides in length, a standard DNA blotting procedure is followed, involving pre-hybridization and hybridization at 42°C in 5X SSPE, 0.3% SDS, 200 μg / ml cleaved and denatured salmon sperm DNA, and 50% formamide for 12 to 24 hours. Finally, the vector material is washed three times at 70°C with 2X SSC and 0.2% SDS for 15 minutes each time.

[0349] Subsequence: The term "subsequence" refers to a polynucleotide that has one or more (e.g., several) nucleotides deleted from the 5' and / or 3' ends of a mature polypeptide coding sequence; wherein the subsequence encodes a fragment having DNase activity. In one aspect, the subsequence comprises at least 550 nucleotides (e.g., 85 to 630 nucleotides of SEQ ID NO: 1, 3, or 5), at least 400 nucleotides (e.g., 100 to 500 nucleotides of SEQ ID NO: 1, 3, or 5), or at least 300 nucleotides (e.g., 200 to 500 nucleotides of SEQ ID NO: 1, 3, or 5).

[0350] textileThe term "textiles" means any textile material including yarns, yarn intermediates, fibers, nonwoven materials, natural materials, synthetic materials, and any other textile materials, fabrics made from these materials, and products (such as clothing and other articles) made from these fabrics. The textiles or fabrics can be in the form of knitted fabrics, woven fabrics, denim, nonwoven fabrics, felt, yarns, and terry cloth. These textiles can be cellulose-based, such as natural cellulose, including cotton, flax / linen, jute, ramie, sisal, or coconut fiber, or man-made cellulose (e.g., derived from wood pulp), including cellulose glue / rayon, cellulose acetate fiber (tricell), lyocell, or blends thereof. Textiles or fabrics can also be non-cellulose-based, such as natural polyamides, including wool, camel hair, cashmere, mohair, rabbit hair, and silk, or synthetic polymers such as nylon, aramid, polyester, acrylic, polypropylene, and spandex / elastane, or blends thereof, as well as blends of their cellulose-based and non-cellulose-based fibers. Examples of blends are blends of cotton and / or rayon / cellulose with one or more accompanying materials, such as wool, synthetic fibers (e.g., polyamide fibers, acrylic fibers, polyester fibers, polyvinyl chloride fibers, polyurethane fibers, polyurea fibers, aramid fibers) and / or cellulose-containing fibers (e.g., rayon / cellulose, ramie, flax / linen, jute, cellulose acetate fibers, lyocell fibers). Fabrics can be conventional washable garments, such as soiled housecloths. When the terms fabric or clothing are used, it is intended to also include the broader term textiles. In the context of this invention, the term "textiles" also includes fabrics.

[0351] variantsThe term "variant" refers to a polypeptide having the same enzymatic activity as the parent enzyme. For example, in this context, a variant of the present invention has DNase activity, wherein the variant contains alterations (i.e., substitutions, insertions, and / or deletions) at one or more (e.g., several) positions. Substitution means replacing an amino acid occupying a position with a different amino acid; deletion means removing an amino acid occupying a position; and insertion means adding an amino acid adjacent to and immediately following the amino acid occupying a position. In the context of the present invention, the identified variant of the DNase has the enzymatic activity of the parent, i.e., the ability to catalyze the hydrolysis and breakage of phosphodiester bonds in the DNA backbone (deoxyribonuclease activity). In one embodiment, the deoxyribonuclease activity of the variant is increased with respect to the mature polypeptide of the parent DNase, such as SEQ ID NO: 2, 4, or 6.In one embodiment, the polypeptide has DNase activity, and the variant has increased DNase activity compared to the parental DNase, such as SEQ ID NO 2, SEQ ID NO 4, SEQ ID NO 6, SEQ ID NO 26, SEQ ID NO 28, SEQ ID NO 30, SEQ ID NO 32, SEQ ID NO 34, SEQ ID NO 36, SEQ ID NO 38, SEQ ID NO 40, SEQ ID NO 42, SEQ ID NO 44, SEQ ID NO 46, SEQ ID NO 48, SEQ ID NO 50, SEQ ID NO 52, SEQ ID NO 55, SEQ ID NO 58, SEQ ID NO 61, SEQ ID NO 64, SEQ ID NO 67, SEQ ID NO 70, SEQ ID NO 73, SEQ ID NO 76, SEQ ID NO 79, SEQ ID NO 82, SEQ ID NO 85, SEQ ID NO 88, SEQ ID NO 91, SEQ ID NO 94, SEQ ID NO 97. SEQ ID NO 100, SEQ ID NO 103, SEQ ID NO 106, SEQ ID NO 109, SEQ ID NO 112, SEQ ID NO 115, SEQ ID NO 118, SEQ ID NO 121, SEQ ID NO 124, SEQ ID NO 127, SEQ ID NO 130, SEQ ID NO 133, SEQ ID NO 136. SEQ ID NO 139, SEQ ID NO 142, SEQ ID NO 145, SEQ ID NO 148, SEQ ID NO 151, SEQ ID NO 154, SEQ ID NO 157, SEQ ID NO 160, SEQ ID NO 163, SEQ ID NO 166, SEQ ID NO 169, SEQ ID NO 172, SEQ ID NO 175, SEQ ID NO 178, SEQ ID NO 181, SEQ ID NO 184, SEQ ID NO 187, SEQ ID Mature polypeptides of NO 190, SEQ ID NO 193 or SEQ ID NO 196.

[0352] Washing cycle:Here, the term "washing cycle" is defined as a washing operation in which textiles are immersed in a washing solution, some mechanical action is applied to the textiles to remove stains and facilitate the flow of washing solution into and out of the textiles, and finally, excess washing solution is removed. After one or more washing cycles, the textiles are generally rinsed and dried.

[0353] Detergent: The term "washing liquid" is defined herein as a solution or mixture of water and detergent composition that optionally includes the enzymes of the present invention.

[0354] Washing time Here, the term "washing time" is defined as the total time taken for a complete wash cycle. That is, the time taken for one or more wash cycles and one or more rinse cycles to be combined.

[0355] Whiteness Here, the term "whiteness" is defined as a broad term with different meanings in different fields and for different customers. Loss of whiteness can be attributed, for example, to ashing, yellowing, or removal of brighteners / toners. Ashing and yellowing can be attributed to dirt redeposition, body dirt, coloring from, for example, iron and copper ions, or dye transfer. Whiteness can include one or more of the following issues: the effect of colorants or dyes; incomplete stain removal (e.g., body dirt, sebum, etc.); redeposition (ashing, yellowing, or other discoloration of an object) (re-associating removed dirt with other parts of the textile (soiled or unsoiled)); chemical changes in the textile during application; and clarification or fading of color.

[0356] Naming conventions

[0357] For the purposes of this invention, the nomenclature [E / Q] indicates that the amino acid at that position can be glutamic acid (Glu, E) or glutamine (Gln, Q). Similarly, the nomenclature [V / G / A / I] indicates that the amino acid at that position can be valine (Val, V), glycine (Gly, G), alanine (Ala, A), or isoleucine (Ile, I), and so on, as other combinations described herein. Unless further limited otherwise, amino acid X is defined such that it can be any of the 20 naturally occurring amino acids. Attached Figure Description

[0358] Figure 1 shows the alignment of the polypeptides of the present invention contained in the GYS clade.

[0359] Figure 2 shows the alignment of the polypeptides of the present invention contained in the NAWK clade.

[0360] Figure 3 shows the alignment of the polypeptides of the present invention contained in the KNAW clade. Detailed Implementation

[0361] This invention relates to novel polypeptides with deoxyribonuclease (DNase) activity, which can be used to prevent, reduce, or remove biofilms on articles such as textiles and / or fabrics. A polypeptide with DNase activity, or deoxyribonuclease (DNase), is any enzyme that catalyzes the hydrolytic cleavage of phosphodiester bonds in the DNA backbone, thereby degrading DNA. The two terms, polypeptide with DNase activity and DNase, are used interchangeably.

[0362] polypeptide

[0363] An example of a polypeptide with DNase activity is DUF1524, which contains the PFAM domain. http: / / pfam.xfam.org / The peptide of DUF1524 is described in “The Pfam protein families database: towards a more sustainable future”, R.D. Finn et al., Nucleic Acids Research (2016), Volume 44: D279-D285. The DUF1524 domain contains a conserved HXXP sequence motif commonly found in nucleases (MA Machnicka et al., Phylogenomics and sequence-structure-function relationships in the GmrSD family of Type IV restriction enzymes, BMC Bioinformatics, 2015, 16). 336). DUF stands for Domain of Unknown Function, and families of polypeptides containing DUFs, for example, are collected together in the Pfam database. The Pfam database provides sequence alignments and Hidden Markov Models for the bounded collection of protein domains. A protein domain is a conserved part of a given protein sequence and (tertiary) structure that can evolve, function, and exist independently of the rest of the protein chain. Each domain forms a compact three-dimensional structure and is generally stable and foldable independently. Many proteins consist of several domains. A domain may appear in a variety of different proteins.

[0364] A specific DUF can be identified using the prefix DUF followed by a number (e.g., 1524). DUF1524 is a family of proteins (all containing the HXXP motif), where H is the amino acid histidine, P is the amino acid proline, and X is any amino acid.

[0365] In one aspect of the invention, the polypeptide of the invention having DNase activity comprises a DUF1524 domain. Therefore, according to one embodiment, the invention relates to polypeptides having DNase activity, wherein these polypeptides comprise a DUF1524 domain, and to the use of such DNases (e.g., for preventing, reducing, or removing biofilms on articles such as textiles and / or fabrics). The invention further relates to compositions comprising polypeptides having DNase activity, such as HXXP, containing a DUF1524 domain. Such compositions may be, but are not limited to, liquid or powder laundry compositions, tablets, unit doses, sprays, or soap bars.

[0366] In one embodiment, the DNAse of the present invention comprises one or more DUF1524 domains, for example, comprising one or both of the following: the novel motif [T / D / S][G / N]PQL (SEQ ID NO 198); [G / T]Y[D / S][R / K / L] (SEQ ID NO: 199); wherein T is threonine, D is aspartic acid, S is serine, G is glycine, N is asparagine, P is proline, Q is glutamine, L is leucine, Y is tyrosine, R is arginine, and K is lysine, i.e., the amino acids are listed in a single letter code. Parentheses indicate alternative amino acids within brackets separated by vertical lines, or in some cases no lines, such as [TDS]. Thus, [T / D / S][G / N]PQL means that T, D, or S may be in the first position, and G or N may be in the second position, followed by PQL. These motifs can be any of TGPQL, TNPQL, DGPQL, DNPQL, SGPQL, or SNPQL. For the motif [G / T]Y[D / S][R / K / L], the conserved amino acid is Y and either the preceding G or T amino acid, and either the following D or S amino acid. Therefore, the motif can be GYD, TYD, GYS, or TYS.

[0367] Another shared domain among the peptides of DUF1524 is [E / D / H]H[I / V / L / F / M]X[P / A / S] (SEQ ID NO 200), located at positions 87 to 91 relative to Bacillus foodosa (SEQ ID NO 21). H88 is a catalytic residue involved in the catalytic activity of DUF1524 and is part of the HXXP motif. Modifying H88 to another amino acid results in the loss of catalytic activity.

[0368] Peptides possessing DNase activity and containing these motifs have exhibited particularly good deep cleaning properties; that is, the DNase-active peptides of the present invention are particularly effective in removing or reducing biofilms. One aspect of the invention relates to peptides possessing DNase activity, wherein these peptides comprise one or more of the motifs [E / D / H]H[I / V / L / F / M]X[P / A / S] (SEQ ID NO: 200), [T / D / S][G / N]PQL (SEQ ID NO: 198), and [G / T]Y[D / S][R / K / L] (SEQ ID NO: 199). One aspect of the present invention relates to polypeptides having DNase activity, wherein such polypeptides comprise one or more of the motifs [E / D / H]H[I / V / L / F / M]X[P / A / S] (SEQ ID NO 200), [T / D / S][G / N]PQL (SEQ ID NO: 198), and [G / T]Y[D / S][R / K / L] (SEQ ID NO: 199), provided that the polypeptide is not a DNase comprising SEQ ID NO 2 of WO 2015 / 155351.

[0369] One embodiment of the present invention relates to a DNase comprising one or more of the DUF1524 domain and motif SEQ ID NO: 198, SEQ ID NO: 199 or SEQ ID NO: 200, wherein the DNase has deep cleaning properties, i.e., wherein the DNase effectively prevents, reduces or removes biofilms on articles such as fabrics, textiles and / or hard surfaces.

[0370] As already described, the polypeptides of the present invention having DNase activity may contain the domain of DUF1524. Additional domains preferably shared by the DNases of the present invention have been identified. This domain, previously undescribed, is referred to as NUC1, and polypeptides containing this domain, in addition to having DNase activity, are characterized by containing one or more of certain motifs, for example, the motifs [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201) or C[D / N]T[A / R] (SEQ ID NO: 202); as described above, the letters indicate amino acids in the single-letter codes, thus F is phenylalanine, L is leucine, A is alanine, N is asparagine, D is aspartic acid, I is isoleucine, V is valine, H is histidine, G is glycine, C is cysteine, T is threonine, R is arginine, etc. Parentheses indicate that the amino acid within the parentheses is a substitute.

[0371] One embodiment of the present invention relates to polypeptides having DNA enzyme activity, wherein such polypeptides comprise one or both of the motifs [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201) or C[D / N]T[A / R] (SEQ ID NO: Y). One embodiment of the present invention relates to polypeptides having DNase activity, wherein these polypeptides comprise one or both of the motifs [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201) or C[D / N]T[A / R] (SEQ ID NO: 202), preferably wherein the motif [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201) is located at positions 110 to 114 corresponding to SEQ ID NO 21 and / or wherein the motif C[D / N]T[A / R] is located at positions 43 to 46 corresponding to SEQ ID NO 21.

[0372] One embodiment of the present invention relates to a polypeptide having DNase activity, wherein such polypeptide comprises one or both of the motifs [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201) or C[D / N]T[A / R] (SEQ ID NO: 202), provided that the polypeptide is not a DNase containing SEQ ID NO 2 of WO 2015 / 155351, preferably the motif [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201) is located at positions 110 to 114 corresponding to SEQ ID NO 21 and / or wherein the motif C[D / N]T[A / R] is located at positions 43 to 46 corresponding to SEQ ID NO 21.

[0373] Motifs and domains are defined as cross-domains, meaning that both the domain and motif encompass both fungal and bacterial DNases. It is well known that DNases belonging to different taxa can share common structural elements, which can be identified by comparing major structures (e.g., amino acid sequences) and grouping DNases based on sequence homology. However, common structural elements can also be identified by comparing the three-dimensional (3D) structures of various DNases. Both methods have been applied in this invention.

[0374] Structural methods have identified DNases that have different taxa but share common structural elements within the identified groups. Groups (such as, for example, clades) share common functions that may be preferential towards certain biomembranes, etc.

[0375] From the NUC1 domain, the inventors have identified a subdomain, which is referred to as the NUC1_A domain. In addition to containing any of the domains described above, polypeptides possessing DNase activity belonging to the NUC1_A domain share a common motif [D / Q][I / V]DH (SEQ ID NO 203), corresponding to amino acids 85 to 88 in a reference polypeptide (SEQ ID NO: 21). The D at position 85 of SEQ ID NO 21 is predicted to involve the catalytic binding of a metal ion cofactor, where the letters define the amino acids as described above, and the brackets indicate alternative amino acids. In one embodiment, the present invention relates to polypeptides containing the motif [D / Q][I / V]DH (SEQ ID NO: 203), wherein these polypeptides possess DNase activity. In another embodiment, the present invention relates to polypeptides containing the motif [D / Q][I / V]DH (SEQ ID NO: 203), wherein these polypeptides possess DNase activity, provided that the polypeptide is not a DNase containing SEQ ID NO 2 of WO 2015 / 155351. In one embodiment, the present invention relates to polypeptides comprising one or more of the following motifs selected from the motifs [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], and [D / Q][I / V]DH, wherein these polypeptides have DNase activity. In one embodiment, the present invention relates to a polypeptide comprising one or more of the following motifs selected from the motifs [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], and [D / Q][I / V]DH, wherein the polypeptide has DNase activity provided that the polypeptide is not a DNase comprising SEQ ID NO 2 of WO 2015 / 155351.

[0376] Polypeptides possessing DNase activity and comprising one or more of the motifs [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], and [D / Q][I / V]DH effectively prevent, remove, or reduce biofilms, and DNases are particularly useful in cleaning processes (e.g., laundry or dishwashing). One aspect of the invention relates to a polypeptide possessing DNase activity, wherein the polypeptide is selected from the group consisting of,The group consists of SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20. SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 53, SEQ ID NO: 56, SEQ ID NO: 59, SEQ ID NO: 62, SEQ ID NO: 68, SEQ ID NO: 71, SEQ ID NO: 74, SEQ ID NO: 77, SEQ ID NO: 83, SEQ ID NO: 86. SEQ ID NO: 89. SEQ ID NO: 92. SEQ ID NO: 95. SEQ ID NO: 98. SEQ ID NO: 101, SEQ ID NO: 104, SEQ ID NO: 107, SEQ ID NO: 110, SEQ ID NO: 113, SEQ ID NO: 116, SEQ ID NO: 119, SEQ ID NO: 128, SEQ ID NO: 131, SEQ ID NO: 134, SEQ ID NO: 137, SEQ ID NO: 140, SEQ ID NO: 143, SEQ ID NO: 146, SEQ ID NO: 149, SEQ ID NO: 152, SEQ ID NO: 155, SEQ ID NO: 158, SEQ ID NO: 164, SEQ ID NO: 167, SEQ ID NO: 170, SEQ ID NO: 173, SEQ ID NO: 176, SEQ ID NO: 179. SEQ ID NO: 182. SEQ ID NO: 185. SEQ ID NO: 188. SEQ ID NO: The polypeptides with amino acid sequences shown in SEQ ID NO: 191 and SEQ ID NO: 197, or the polypeptides having at least 80% sequence identity with them (e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity), are considered to constitute a polypeptide.The polypeptide further comprises one or all of the following motifs: [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], and [D / Q][I / V]DH. These motifs are novel and have not been previously described. Therefore, the DNases of the present invention share a novel common inventive concept.

[0377] One aspect of the present invention relates to a polypeptide having DNase activity, wherein the polypeptide comprises one or all of the motifs [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], or [D / Q][I / V]DH, wherein the polypeptide is selected from the group consisting of the following items: a) A polypeptide having at least 84% sequence identity with the polypeptide of SEQ ID NO: 8. b) A polypeptide having at least 94% sequence identity with the polypeptide of SEQ ID NO: 9. c) A polypeptide having at least 92% sequence identity with the polypeptide of SEQ ID NO: 11. d) A polypeptide having at least 92% sequence identity with the polypeptide of SEQ ID NO: 12. e) A polypeptide having at least 97% sequence identity with the polypeptide of SEQ ID NO: 13. f) A polypeptide having at least 96% sequence identity with the polypeptide of SEQ ID NO: 14. g) A polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 15. h) A polypeptide having at least 88% sequence identity with the polypeptide of SEQ ID NO: 16. i) A polypeptide having at least 93% sequence identity with the polypeptide of SEQ ID NO: 17. j) A polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 18. k) A polypeptide having at least 89% sequence identity with the polypeptide of SEQ ID NO: 19. l) A polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 20. m) A polypeptide with at least 93% sequence identity to the polypeptide of SEQ ID NO: 21. n) A polypeptide that has at least 90% sequence identity with the polypeptide of SEQ ID NO: 22. o) A polypeptide having at least 93% sequence identity with the polypeptide of SEQ ID NO: 23. p) A polypeptide having at least 85% sequence identity with the polypeptide of SEQ ID NO: 53. q) A polypeptide having at least 98% sequence identity with the polypeptide of SEQ ID NO: 56. r) A polypeptide having at least 94% sequence identity with the polypeptide of SEQ ID NO: 59. s) A polypeptide having at least 85% sequence identity with the polypeptide of SEQ ID NO: 62. t) A polypeptide with at least 97% sequence identity to the polypeptide of SEQ ID NO: 68. u) A polypeptide having at least 99% sequence identity with the polypeptide of SEQ ID NO: 71. v) A polypeptide with at least 91% sequence identity to the polypeptide of SEQ ID NO: 74. w) A polypeptide with at least 98% sequence identity to the polypeptide of SEQ ID NO: 77. x) A polypeptide having at least 81% sequence identity with the polypeptide of SEQ ID NO: 83. y) A polypeptide having at least 89% sequence identity with the polypeptide of SEQ ID NO: 86. z) A polypeptide having at least 99% sequence identity with the polypeptide of SEQ ID NO: 89. aa) A polypeptide that has at least 91% sequence identity with the polypeptide of SEQ ID NO: 92. (bb) A polypeptide that has at least 90% sequence identity with the polypeptide of SEQ ID NO: 95. (cc) A polypeptide with at least 91% sequence identity to the polypeptide of SEQ ID NO: 98. (dd) A polypeptide with at least 92% sequence identity to the polypeptide of SEQ ID NO: 104. (ee) A polypeptide that has at least 90% sequence identity with the polypeptide of SEQ ID NO: 107.

[0378] (ff) A polypeptide having at least 91.5% sequence identity with the polypeptide of SEQ ID NO: 110.

[0379] A polypeptide (gg) that has at least 93% sequence identity with the polypeptide of SEQ ID NO: 113. (hh) A polypeptide that has at least 96% sequence identity with the polypeptide of SEQ ID NO: 116. ii) A polypeptide having at least 99.5% sequence identity with the polypeptide of SEQ ID NO: 119. (jj) A polypeptide with at least 99.5% sequence identity to the polypeptide of SEQ ID NO: 128. A polypeptide that has at least 93% sequence identity with the polypeptide of SEQ ID NO: 131. ll) A polypeptide having at least 79% sequence identity with the polypeptide of SEQ ID NO: 134. A polypeptide (mm) having at least 72% sequence identity with the polypeptide of SEQ ID NO: 137. (nn) A polypeptide having at least 77% sequence identity with the polypeptide of SEQ ID NO: 140. (oo) A polypeptide with at least 74% sequence identity to the polypeptide of SEQ ID NO: 143. A polypeptide (pp) having at least 97% sequence identity with the polypeptide of SEQ ID NO: 146. A polypeptide (qq) having at least 71% sequence identity with the polypeptide of SEQ ID NO: 149. (rr) A polypeptide having at least 96% sequence identity with the polypeptide of SEQ ID NO: 152. A polypeptide that has at least 72% sequence identity with the polypeptide of SEQ ID NO: 155. (tt) A polypeptide with at least 93% sequence identity to the polypeptide of SEQ ID NO: 158. The polypeptide (uu) has at least 85% sequence identity with the polypeptide of SEQ ID NO: 164. A polypeptide that has at least 88% sequence identity with the polypeptide of SEQ ID NO: 167. A polypeptide that has at least 87% sequence identity with the polypeptide of SEQ ID NO: 176. (xx) A polypeptide with at least 81% sequence identity to the polypeptide of SEQ ID NO: 179. (yy) is a polypeptide that has at least 89% sequence identity with the polypeptide of SEQ ID NO: 182. (zz) A polypeptide with at least 96% sequence identity to the polypeptide of SEQ ID NO: 185. aaa) is a polypeptide that has at least 88% sequence identity with the polypeptide of SEQ ID NO: 188. (bbb) A polypeptide that has at least 87% sequence identity with the polypeptide of SEQ ID NO: 191. A polypeptide having at least 94% sequence identity with the polypeptide of SEQ ID NO: 194, and (ddd) A polypeptide that has at least 90% sequence identity with the polypeptide of SEQ ID NO: 197.

[0380] The NUC_1A domain was first identified in this invention and described above. This domain can be further divided into different clades. A clade is a group of polypeptides grouped together based on homology characteristics traced back to a common ancestor. A polypeptide clade can be viewed as a phylogenetic tree, where a clade is a group of polypeptides consisting of a common ancestor and all its direct descendants. Example 11 illustrates the generation of a phylogenetic tree.

[0381] A GYS clade, or GYS-clade, is a group of DNases that are all related to a common ancestor and share common characteristics. Unknown clades here comprise polypeptides that form groups within domains (e.g., NUC1_A in a phylogenetic tree) that share common characteristics and are more closely related than other polypeptides in that domain.

[0382] Peptides of the GYS clade share the conserved motif [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), where the letter represents an amino acid (a one-letter code), X represents any amino acid, and the brackets indicate that the amino acid is a substitute. Furthermore, peptides of the GYS clade may contain any one of the motifs [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], and [D / Q][I / V]DH.

[0383] One aspect of the invention relates to polypeptides of the GYS clade comprising one or more of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein these polypeptides possess DNase activity. In one aspect, the ASXNRSKG motif corresponds to positions 125 to 133 of SEQ ID NO 21. In another aspect, the [D / M / L][S / T]GYSR[D / N] motif corresponds to positions 26 to 32 of SEQ ID NO 21.

[0384] The GYS clade comprises polypeptides with DNase activity as shown in SEQ ID NO 8, SEQ ID NO 9, SEQ ID NO 11, SEQ ID NO 12, SEQ ID NO 13, SEQ ID NO 14, SEQ ID NO 15, SEQ ID NO 16, SEQ ID NO 17, SEQ ID NO 18, SEQ ID NO 19, SEQ ID NO 20, SEQ ID NO 21, SEQ ID NO 22, SEQ ID NO 23, SEQ ID NO 53, SEQ ID NO 56, SEQ ID NO 59, SEQ ID NO 62, SEQ ID NO 65, SEQ ID NO 68, SEQ ID NO 71, SEQ ID NO 74, SEQ ID NO 77 and SEQ ID NO 80.

[0385] The polypeptides with DNase activity shown in SEQ ID NO 65 and SEQ ID NO 80 are common sequences with UniProt accession numbers (H6NAU2 and A0A0M2T1U6).

[0386] One aspect of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) and ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is selected from SEQ ID NO 8, SEQ ID NO 9, SEQ ID NO 11, SEQ ID NO 12, SEQ ID NO 13, SEQ ID NO 14, SEQ ID NO 15, SEQ ID NO 16, SEQ ID NO 17, SEQ ID NO 18, SEQ ID NO 19, SEQ ID NO 20, SEQ ID NO 21, SEQ ID NO 22, SEQ ID NO 23, SEQ ID NO 53, SEQ ID NO 56, SEQ ID NO 59, SEQ ID NO 62, SEQ ID NO 68, SEQ ID NO 71, SEQ ID NO 74 and SEQ ID NO Any of the polypeptides shown in 77 or a variant thereof having 1-25 (e.g., 1-20, 1-15, 1-10, 1-5) amino acid changes (e.g., substitutions).

[0387] One aspect of the present invention relates to a polypeptide of the GYS clade, the polypeptide comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N], ASXNRSKG, and further comprising one or more of the motifs [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], and [D / Q][I / V]DH, wherein the polypeptide has DNase activity and wherein the polypeptide is selected from SEQ ID NO 8, SEQ ID NO 9, SEQ ID NO 11, SEQ ID NO 12, SEQ ID NO 13, SEQ ID NO 14, SEQ ID NO 15, SEQ ID NO 16, SEQ ID NO 17, SEQ ID NO 18, SEQ ID NO 19, SEQ ID NO 10 ... Any of the polypeptides shown in SEQ ID NO 19, SEQ ID NO 20, SEQ ID NO 21, SEQ ID NO 22, SEQ ID NO 23, SEQ ID NO 53, SEQ ID NO 56, SEQ ID NO 59, SEQ ID NO 62, SEQ ID NO 68, SEQ ID NO 71, SEQ ID NO 74 and SEQ ID NO 77, or a variant thereof having 1-25 (e.g. 1-20, 1-15, 1-10, 1-5) amino acid changes (e.g. substitutions).

[0388] One embodiment of the present invention relates to a polypeptide of the GYS clade, the polypeptide having DNase activity and comprising one or more of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) and ASXNRSKG (SEQ ID NO: 205), provided that the polypeptide is not the polypeptide shown in SEQ ID NO 65 or SEQ ID NO 80.

[0389] One embodiment of the present invention relates to a polypeptide of the GYS clade, the polypeptide having DNase activity, wherein the polypeptide comprises one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) and ASXNRSKG (SEQ ID NO: 205), and wherein the polypeptide is selected from the following polypeptides: a) A polypeptide having at least 84% sequence identity with the polypeptide shown in SEQ ID NO: 8. b) A polypeptide having at least 94% sequence identity with the polypeptide shown in SEQ ID NO: 9. c) A polypeptide having at least 92% sequence identity with the polypeptide shown in SEQ ID NO: 11. d) A polypeptide having at least 92% sequence identity with the polypeptide shown in SEQ ID NO: 12. e) A polypeptide having at least 97% sequence identity with the polypeptide shown in SEQ ID NO: 13. f) A polypeptide having at least 96% sequence identity with the polypeptide shown in SEQ ID NO: 14. g) A polypeptide having at least 90% sequence identity with the polypeptide shown in SEQ ID NO: 15. h) A polypeptide having at least 88% sequence identity with the polypeptide shown in SEQ ID NO: 16. i) A polypeptide having at least 93% sequence identity with the polypeptide shown in SEQ ID NO: 17. j) A polypeptide having at least 90% sequence identity with the polypeptide shown in SEQ ID NO: 18. k) A polypeptide having at least 89% sequence identity with the polypeptide shown in SEQ ID NO: 19. l) A polypeptide having at least 90% sequence identity with the polypeptide shown in SEQ ID NO: 20. m) A polypeptide having at least 93% sequence identity with the polypeptide shown in SEQ ID NO: 21. n) A polypeptide having at least 90% sequence identity with the polypeptide shown in SEQ ID NO: 22. o) A polypeptide having at least 93% sequence identity with the polypeptide shown in SEQ ID NO: 23. p) A polypeptide having at least 85% sequence identity with the polypeptide shown in SEQ ID NO: 53. q) A polypeptide having at least 98% sequence identity with the polypeptide shown in SEQ ID NO: 56. r) A polypeptide having at least 94% sequence identity with the polypeptide shown in SEQ ID NO: 59. s) A polypeptide having at least 85% sequence identity with the polypeptide shown in SEQ ID NO: 62. t) A polypeptide having at least 97% sequence identity with the polypeptide shown in SEQ ID NO: 68. u) A polypeptide having at least 99% sequence identity with the polypeptide shown in SEQ ID NO: 71. v) A polypeptide having at least 91% sequence identity with the polypeptide shown in SEQ ID NO: 74, and w) A polypeptide having at least 98% sequence identity with the polypeptide shown in SEQ ID NO: 77.

[0390] One embodiment of the present invention relates to a polypeptide of the GYS clade, the polypeptide having DNase activity and wherein the polypeptide comprises one or more of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) and ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide having DNase activity is selected from the group consisting of: SEQ ID NO 8, SEQ ID NO 9, SEQ ID NO 11, SEQ ID NO 12, SEQ ID NO 13, SEQ ID NO 14, SEQ ID NO 15, SEQ ID NO 16, SEQ ID NO 17, SEQ ID NO 18, SEQ ID NO 19, SEQ ID NO 20, SEQ ID NO 21, SEQ ID NO 22, SEQ ID NO 23, SEQ ID NO 53, SEQ ID NO 56, SEQ ID NO 59, SEQ ID NO 62, SEQ ID NO 68, SEQ ID NO 71, SEQ ID NO 74 and SEQ ID NO 77 or a polypeptide that has at least 99% sequence identity with it.

[0391] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 8 or a polypeptide having at least 84%, for example at least 85%, for example at least 90%, for example at least 95%, or for example at least 100% sequence identity with it.

[0392] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is shown in SEQ ID NO 9 or has at least 94%, for example at least 95%, or for example at least 100% sequence identity with it.

[0393] One embodiment of the present invention relates to a polypeptide of the GYS clade, the polypeptide comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide of SEQ ID NO 11 or a polypeptide having at least 92%, for example at least 95%, for example at least 96%, for example at least 98%, or for example at least 100% sequence identity with it.

[0394] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 12 or a polypeptide having at least 92%, for example at least 95%, for example at least 97%, for example at least 98%, or for example at least 100% sequence identity with it.

[0395] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 13 or a polypeptide having at least 97%, for example at least 98%, for example at least 99%, or for example at least 100% sequence identity with it.

[0396] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 14 or a polypeptide having at least 96%, for example at least 97%, for example at least 98%, for example at least 99%, or for example at least 100% sequence identity with it.

[0397] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 15 or a polypeptide having at least 90%, for example at least 93%, for example at least 95%, for example at least 97%, or for example at least 100% sequence identity with it.

[0398] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 16 or a polypeptide having at least 88%, for example, at least 90%, for example, at least 93%, for example, at least 95%, for example, at least 98%, or for example, at least 100% sequence identity with it.

[0399] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 17 or a polypeptide having at least 93%, for example at least 95%, for example at least 96%, for example at least 97%, for example at least 98%, or for example at least 100% sequence identity with it.

[0400] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 18 or a polypeptide having at least 90%, for example at least 91%, for example at least 93%, for example at least 95%, for example at least 98%, or for example at least 100% sequence identity with it.

[0401] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 21), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 19 or a polypeptide having at least 89%, for example, at least 90%, for example, at least 93%, for example, at least 95%, for example, at least 98%, or for example, at least 100% sequence identity with it.

[0402] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 20 or a polypeptide having at least 90%, for example at least 91%, for example at least 93%, for example at least 95%, for example at least 98%, or for example at least 100% sequence identity with it.

[0403] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 21 or a polypeptide having at least 93%, for example at least 94%, for example at least 95%, for example at least 96%, for example at least 97%, for example at least 98%, or for example at least 100% sequence identity with it.

[0404] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 22 or a polypeptide having at least 90% (at least 93%, for example at least 94%, for example at least 95%, for example at least 96%, for example at least 97%, for example at least 98%, or for example at least 100%) sequence identity with it.

[0405] One embodiment of the present invention relates to a polypeptide of the GYS clade, the polypeptide comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 23 or a polypeptide having at least 93%, for example at least 94%, for example at least 95%, for example at least 96%, for example at least 97%, for example at least 98%, for example at least 99%, or for example at least 100% sequence identity with it.

[0406] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptides have DNase activity and wherein the polypeptides are the polypeptide shown in SEQ ID NO 53 or a polypeptide having at least 85% (at least 90%, for example at least 93%, for example at least 95%, for example at least 96%, for example at least 97%, for example at least 98%, or for example at least 100%) sequence identity with it.

[0407] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 56 or a polypeptide having at least 98%, for example at least 99%, or for example at least 100% sequence identity with it.

[0408] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 59 or a polypeptide having at least 94%, for example at least 95%, for example at least 96%, for example at least 97%, for example at least 98%, or for example at least 100% sequence identity with it.

[0409] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or more of the motif [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 62 or a polypeptide having at least 85% (at least 90%, for example at least 95%, for example at least 95%, for example at least 96%, for example at least 97%, for example at least 98%, or for example at least 100%) sequence identity with it.

[0410] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or more of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 68 or a polypeptide having at least 97%, for example at least 98%, or for example at least 100% sequence identity with it.

[0411] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or more of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 71 or a polypeptide having at least 99% or 100% sequence identity with it.

[0412] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or more of the motif [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 74 or a polypeptide having at least 91% (at least 93%, for example at least 94%, for example at least 95%, for example at least 96%, for example at least 97%, for example at least 98%, or for example at least 100%) sequence identity with it.

[0413] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or more of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 77 or a polypeptide having at least 98%, for example at least 99% or 100% sequence identity with it.

[0414] Another distinguishable clade is the NAWK clade. The NAWK clade, or NAWK clade, is a group of DNases that are all related to a common ancestor and share common characteristics. Unknown clades here consist of polypeptides that form groups within domains (e.g., NUC1_A in a phylogenetic tree) that can share common characteristics and are more closely related than other polypeptides in that domain.

[0415] Peptides of the NAWK clade share the conserved motif [V / I]PL[S / A]NAWK (SEQ ID NO: 206) or NPQL (SEQ ID NO: 207), where the letters are amino acids (one-letter codes) and the amino acids in parentheses are substitutes. Furthermore, peptides of the NAWK clade may contain any one of the motifs [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], and [D / Q][I / V]DH.

[0416] One aspect of the invention relates to polypeptides of the NAWK clade, these polypeptides comprising one or more of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) or NPQL (SEQ ID NO: 207), wherein these polypeptides possess DNase activity. In one aspect, the [VI]PL[S / A]NAWK motif corresponds to positions 87 to 94 of SEQ ID NO 68. In another aspect, the NPQL motif corresponds to positions 114 to 117 of SEQ ID NO 68.

[0417] The NAWK clade includes polypeptides with DNase activity as shown in SEQ ID NO 83, SEQ ID NO 86, SEQ ID NO 89, SEQ ID NO 92, SEQ ID NO 95, SEQ ID NO 98, SEQ ID NO 101, SEQ ID NO 104, SEQ ID NO 107, SEQ ID NO 110, SEQ ID NO 113, SEQ ID NO 116 and SEQ ID NO 119.

[0418] The polypeptide shown in SEQ ID NO 119 has 99.48% sequence identity with the polypeptide of UniProt sequence with accession number A0A178DM75.

[0419] One aspect of the invention relates to a polypeptide of the NAWK clade comprising one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) or NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity and wherein the polypeptide is selected from any one of the polypeptides shown in SEQ ID NO 83, SEQ ID NO 86, SEQ ID NO 89, SEQ ID NO 92, SEQ ID NO 95, SEQ ID NO 98, SEQ ID NO 101, SEQ ID NO 104, SEQ ID NO 107, SEQ ID NO 110, SEQ ID NO 113, SEQ ID NO 116 and SEQ ID NO 119 or a variant thereof having 1-25 (e.g. 1-20, 1-15, 1-10, 1-5) amino acid alterations (e.g., substitutions).

[0420] One aspect of the present invention relates to a polypeptide of the NAWK clade, the polypeptide comprising one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) or NPQL (SEQ ID NO: 207) and further comprising one or more of the motifs [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], and [D / Q][I / V]DH, wherein the polypeptide has DNase activity and wherein the polypeptide is selected from SEQ ID NO 83, SEQ ID NO 86, SEQ ID NO 89, SEQ ID NO 92, SEQ ID NO 95, SEQ ID NO 98, SEQ ID NO 101, SEQ ID NO Any of the polypeptides shown in SEQ ID NO 104, SEQ ID NO 107, SEQ ID NO 110, SEQ ID NO 113, SEQ ID NO 116 and SEQ ID NO 119 or a variant thereof having 1-25 (e.g. 1-20, 1-15, 1-10, 1-5) amino acid changes (e.g. substitutions).

[0421] One embodiment of the present invention relates to a polypeptide of the NAWK clade, the polypeptide having DNase activity and comprising one or more of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) and NPQL (SEQ ID NO: 207), provided that the polypeptide is not the polypeptide shown in SEQ ID NO 119.

[0422] One embodiment of the present invention relates to a polypeptide of the NAWK clade, the polypeptide having DNase activity and wherein the polypeptide comprises one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) or NPQL (SEQ ID NO: 207), wherein the polypeptide is selected from the following polypeptides: a) A polypeptide having at least 81% sequence identity with the polypeptide shown in SEQ ID NO: 83. b) A polypeptide having at least 88.5% sequence identity with the polypeptide shown in SEQ ID NO: 86. c) A polypeptide having at least 99% sequence identity with the polypeptide shown in SEQ ID NO: 89 d) A polypeptide having at least 91% sequence identity with the polypeptide shown in SEQ ID NO: 92. e) A polypeptide having at least 90% sequence identity with the polypeptide shown in SEQ ID NO: 95. f) A polypeptide having at least 91% sequence identity with the polypeptide shown in SEQ ID NO: 98. g) A polypeptide having at least 89% sequence identity with the polypeptide shown in SEQ ID NO: 101. h) A polypeptide having at least 92% sequence identity with the polypeptide shown in SEQ ID NO: 104. i) A polypeptide having at least 90% sequence identity with the polypeptide shown in SEQ ID NO: 107 j) A polypeptide having at least 91.5% sequence identity with the polypeptide shown in SEQ ID NO: 110. k) A polypeptide having at least 93% sequence identity with the polypeptide shown in SEQ ID NO: 113, and l) A polypeptide having at least 96% sequence identity with the polypeptide shown in SEQ ID NO: 116. One embodiment of the present invention relates to a polypeptide of the NAWK clade, the polypeptide comprising one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) and NPQL (SEQ ID NO: 207), wherein the polypeptides have DNase activity and wherein the polypeptides are selected from the group consisting of: SEQ ID NO 83, SEQ ID NO 86, SEQ ID NO 92, SEQ ID NO 95, SEQ ID NO 98, SEQ ID NO 101, SEQ ID NO 104, SEQ ID NO 107, SEQ ID NO 110, SEQ ID NO 113 and SEQ ID NO 116 or polypeptides having at least 96% sequence identity with them.

[0423] One embodiment of the present invention relates to a polypeptide of the NAWK clade, the polypeptide comprising one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) and NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 83 or a polypeptide having at least 81%, for example at least 83%, for example at least 85%, for example at least 87%, for example at least 90%, for example at least 93%, for example at least 95%, for example at least 97%, for example at least 98%, for example at least 99%, or 100% sequence identity with it.

[0424] One embodiment of the present invention relates to a polypeptide of the NAWK clade, the polypeptide comprising one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) and NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 86 or a polypeptide having at least 88.5%, for example at least 90%, for example at least 93%, for example at least 95%, for example at least 97%, for example at least 98%, for example at least 99%, or 100% sequence identity with it.

[0425] One embodiment of the present invention relates to a polypeptide of the NAWK clade, the polypeptide comprising one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) and NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 89 or a polypeptide having at least 99% or 100% sequence identity with it.

[0426] One embodiment of the present invention relates to a polypeptide of the NAWK clade, the polypeptide comprising one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) and NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 92 or a polypeptide having at least 91%, for example at least 92%, for example at least 93%, for example at least 95%, for example at least 97%, for example at least 98%, for example at least 99%, or 100% sequence identity with it.

[0427] One embodiment of the present invention relates to a polypeptide of the NAWK clade, the polypeptide comprising one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) and NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 95 or a polypeptide having at least 90%, for example at least 92%, for example at least 93%, for example at least 95%, for example at least 97%, for example at least 98%, for example at least 99%, or 100% sequence identity with it.

[0428] One embodiment of the present invention relates to a polypeptide of the NAWK clade, the polypeptide comprising one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) and NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 98 or a polypeptide having at least 91%, for example at least 92%, for example at least 93%, for example at least 95%, for example at least 97%, for example at least 98%, for example at least 99%, or 100% sequence identity with it.

[0429] One embodiment of the present invention relates to a polypeptide of the NAWK clade, the polypeptide comprising one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) and NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 101 or a polypeptide having at least 89%, for example, at least 90%, for example, at least 93%, for example, at least 95%, for example, at least 97%, for example, at least 98%, for example, at least 99%, or 100% sequence identity with it.

[0430] One embodiment of the present invention relates to a polypeptide of the NAWK clade, the polypeptide comprising one or more of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 68) and NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 104 or a polypeptide having at least 92%, for example at least 93%, for example at least 95%, for example at least 97%, for example at least 98%, for example at least 99%, or 100% sequence identity with it.

[0431] One embodiment of the present invention relates to a polypeptide of the NAWK clade, the polypeptide comprising one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) and NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 107 or a polypeptide having at least 90%, for example at least 92%, for example at least 93%, for example at least 95%, for example at least 97%, for example at least 98%, for example at least 99%, or 100% sequence identity with it.

[0432] One embodiment of the present invention relates to a polypeptide of the NAWK clade, the polypeptide comprising one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) and NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 110 or a polypeptide having at least 91.5%, for example at least 92%, for example at least 93%, for example at least 95%, for example at least 97%, for example at least 98%, for example at least 99%, or 100% sequence identity with it.

[0433] One embodiment of the present invention relates to a polypeptide of the NAWK clade, the polypeptide comprising one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) and NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 113 or a polypeptide having at least 93%, for example at least 94%, for example at least 95%, for example at least 97%, for example at least 98%, for example at least 99%, or 100% sequence identity with it.

[0434] One embodiment of the present invention relates to a polypeptide of the NAWK clade, the polypeptide comprising one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) and NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 116 or a polypeptide having at least 96%, for example at least 97%, for example at least 98%, for example at least 99%, or 100% sequence identity with it.

[0435] One embodiment of the present invention relates to a polypeptide of the NAWK clade, the polypeptide comprising one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) and NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity and wherein the polypeptide comprises or is composed of the polypeptide shown in SEQ ID NO 119.

[0436] The third distinct clade is the KNAW clade. The KNAW clade, or KNAW-clade, is a group of DNases that are all related to a common ancestor and share common characteristics. Unknown clades here comprise polypeptides that form groups within domains (e.g., NUC1_A in a phylogenetic tree) that can share common characteristics and are more closely related than other polypeptides in that domain.

[0437] Peptides of the KNAW clade share the conserved motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) and [K / H / E]NAW (SEQ ID NO: 209), where the letters are amino acids (one-letter codes) and the amino acids in parentheses are substitutions. Furthermore, peptides of the KNAW clade may contain any one of the motifs [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], or [D / Q][I / V]DH.

[0438] One aspect of the invention relates to polypeptides of the KNAW clade, wherein these polypeptides comprise one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) and / or [K / H / E]NAW (SEQ ID NO: 209), wherein these polypeptides have DNase activity.

[0439] The KNAW clade includes polypeptides with DNase activity as shown in SEQ ID NO 122, SEQ ID NO 125, SEQ ID NO 128, SEQ ID NO 131, SEQ ID NO 134, SEQ ID NO 137, SEQ ID NO 140, SEQ ID NO 143, SEQ ID NO 146, SEQ ID NO 149, SEQ ID NO 152, SEQ ID NO 155 and SEQ ID NO 158.

[0440] The polypeptides shown in SEQ ID NO 122 and SEQ ID NO 125 are common sequences.

[0441] One aspect of the invention relates to a polypeptide of the KNAW clade comprising one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity and wherein the polypeptide is selected from any one of the polypeptides shown in SEQ ID NO 128, SEQ ID NO 131, SEQ ID NO 134, SEQ ID NO 137, SEQ ID NO 140, SEQ ID NO 143, SEQ ID NO 146, SEQ ID NO 149, SEQ ID NO 152, SEQ ID NO 155 and SEQ ID NO 158 or a variant thereof having 1-25 (e.g. 1-20, 1-15, 1-10, 1-5) amino acid alterations (e.g., substitutions).

[0442] One aspect of the present invention relates to a polypeptide of the KNAW clade, the polypeptide comprising one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), and further comprising one or more of the motifs [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], and [D / Q][I / V]DH, wherein the polypeptide has DNase activity and wherein the polypeptide is selected from SEQ ID NO 128, SEQ ID NO 131, SEQ ID NO 134, SEQ ID NO 137, SEQ ID NO 140, SEQ ID NO 143, SEQ ID NO Any of the polypeptides shown in SEQ ID NO 146, SEQ ID NO 149, SEQ ID NO 152, SEQ ID NO 155 and SEQ ID NO 158 or a variant thereof having 1-25 (e.g. 1-20, 1-15, 1-10, 1-5) amino acid changes (e.g. substitutions).

[0443] One embodiment of the present invention relates to a polypeptide of the KNAW clade, the polypeptide having DNase activity and wherein the polypeptide comprises one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), provided that the polypeptide is not the polypeptide shown in SEQ ID NO 122 and SEQ ID NO 125 and provided that the polypeptide is not the Trichoderma harzianum DNase shown in SEQ ID NO 2 of WO 2015 / 155351.

[0444] One embodiment of the present invention relates to a polypeptide of the KNAW clade, the polypeptide having DNase activity and wherein the polypeptide comprises one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), and wherein the polypeptide is selected from the following polypeptides: a) A polypeptide having at least 99.5% sequence identity with the polypeptide shown in SEQ ID NO: 128. b) A polypeptide having at least 93% sequence identity with the polypeptide shown in SEQ ID NO: 131. c) A polypeptide having at least 79% sequence identity with the polypeptide shown in SEQ ID NO: 134. d) A polypeptide having at least 72% sequence identity with the polypeptide shown in SEQ ID NO: 137. e) A polypeptide having at least 77% sequence identity with the polypeptide shown in SEQ ID NO: 140. f) A polypeptide having at least 74% sequence identity with the polypeptide shown in SEQ ID NO: 143. g) A polypeptide having at least 97% sequence identity with the polypeptide shown in SEQ ID NO: 146. h) A polypeptide having at least 71% sequence identity with the polypeptide shown in SEQ ID NO: 149. i) A polypeptide having at least 96% sequence identity with the polypeptide shown in SEQ ID NO: 152. j) A polypeptide having at least 72% sequence identity with the polypeptide shown in SEQ ID NO: 155, and k) A polypeptide having at least 98% sequence identity with the polypeptide shown in SEQ ID NO: 158.

[0445] One embodiment of the present invention relates to a polypeptide of the KNAW clade, the polypeptide comprising one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide having DNase activity is selected from the group consisting of: SEQ ID NO 131, SEQ ID NO 134, SEQ ID NO 137, SEQ ID NO 140, SEQ ID NO 143, SEQ ID NO 146, SEQ ID NO 149, SEQ ID NO 152, SEQ ID NO 155 and SEQ ID NO 158 or a polypeptide having at least 98% sequence identity with it.

[0446] One embodiment of the present invention relates to a polypeptide of the KNAW clade, the polypeptide comprising one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 128 or a polypeptide having at least 99.5% or 100% sequence identity with it.

[0447] One embodiment of the present invention relates to a polypeptide of the KNAW clade, the polypeptide comprising one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 131 or a polypeptide having at least 93%, for example at least 94%, for example at least 95%, for example at least 98%, for example at least 99%, or 100% sequence identity with it.

[0448] One embodiment of the present invention relates to a polypeptide of the KNAW clade, the polypeptide comprising one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 134 or a polypeptide having at least 79%, for example, at least 80%, for example, at least 85%, for example, at least 86%, for example, at least 88%, for example, at least 90%, for example, at least 93%, for example, at least 95%, for example, at least 98%, for example, at least 99%, or 100% sequence identity with it.

[0449] One embodiment of the present invention relates to a polypeptide of the KNAW clade, the polypeptide comprising one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 137 or a polypeptide having at least 72%, for example, at least 75%, for example, at least 80%, for example, at least 85%, for example, at least 86%, for example, at least 88%, for example, at least 90%, for example, at least 93%, for example, at least 95%, for example, at least 98%, for example, at least 99%, or 100% sequence identity with it.

[0450] One embodiment of the present invention relates to a polypeptide of the KNAW clade, the polypeptide comprising one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 140 or a polypeptide having at least 77%, for example, at least 80%, for example, at least 85%, for example, at least 86%, for example, at least 88%, for example, at least 90%, for example, at least 93%, for example, at least 95%, for example, at least 98%, for example, at least 99%, or 100% sequence identity with it.

[0451] One embodiment of the present invention relates to a polypeptide of the KNAW clade, the polypeptide comprising one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 143 or a polypeptide having at least 74%, for example, at least 80%, for example, at least 85%, for example, at least 86%, for example, at least 88%, for example, at least 90%, for example, at least 93%, for example, at least 95%, for example, at least 98%, for example, at least 99%, or 100% sequence identity with it.

[0452] One embodiment of the present invention relates to a polypeptide of the KNAW clade, the polypeptide comprising one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 146 or a polypeptide having at least 97%, for example at least 98%, for example at least 99%, or 100% sequence identity with it.

[0453] One embodiment of the present invention relates to a polypeptide of the KNAW clade, the polypeptide comprising one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 149 or a polypeptide having at least 71%, for example, at least 75%, for example, at least 80%, for example, at least 85%, for example, at least 86%, for example, at least 88%, for example, at least 90%, for example, at least 93%, for example, at least 95%, for example, at least 98%, for example, at least 99%, or 100% sequence identity with it.

[0454] One embodiment of the present invention relates to a polypeptide of the KNAW clade, the polypeptide comprising one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 152 or a polypeptide having at least 96%, for example at least 97%, for example at least 98%, for example at least 99%, or 100% sequence identity with it.

[0455] One embodiment of the present invention relates to a polypeptide of the KNAW clade, the polypeptide comprising one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 155 or a polypeptide having at least 72%, for example, at least 75%, for example, at least 80%, for example, at least 85%, for example, at least 86%, for example, at least 88%, for example, at least 90%, for example, at least 93%, for example, at least 95%, for example, at least 98%, for example, at least 99%, or 100% sequence identity with it.

[0456] One embodiment of the present invention relates to a polypeptide of the KNAW clade, the polypeptide comprising one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity and wherein the polypeptide is the polypeptide shown in SEQ ID NO 158 or a polypeptide having at least 98%, for example at least 99% or 100% sequence identity with it.

[0457] One embodiment of the present invention relates to a polypeptide having DNase activity, wherein the polypeptide is selected from the group consisting of the following items: a) A polypeptide having at least 84% sequence identity with the polypeptide shown in SEQ ID NO: 8. b) A polypeptide having at least 94% sequence identity with the polypeptide shown in SEQ ID NO: 9. c) A polypeptide having at least 92% sequence identity with the polypeptide shown in SEQ ID NO: 11. d) A polypeptide having at least 92% sequence identity with the polypeptide shown in SEQ ID NO: 12. e) A polypeptide having at least 97% sequence identity with the polypeptide shown in SEQ ID NO: 13. f) A polypeptide having at least 96% sequence identity with the polypeptide shown in SEQ ID NO: 14. g) A polypeptide having at least 90% sequence identity with the polypeptide shown in SEQ ID NO: 15. h) A polypeptide having at least 88% sequence identity with the polypeptide shown in SEQ ID NO: 16. i) A polypeptide having at least 93% sequence identity with the polypeptide shown in SEQ ID NO: 17. j) A polypeptide having at least 90% sequence identity with the polypeptide shown in SEQ ID NO: 18. k) A polypeptide having at least 89% sequence identity with the polypeptide shown in SEQ ID NO: 19. l) A polypeptide having at least 90% sequence identity with the polypeptide shown in SEQ ID NO: 20. m) A polypeptide having at least 93% sequence identity with the polypeptide shown in SEQ ID NO: 21. n) A polypeptide having at least 90% sequence identity with the polypeptide shown in SEQ ID NO: 22. o) A polypeptide having at least 93% sequence identity with the polypeptide shown in SEQ ID NO: 23. p) A polypeptide having at least 85% sequence identity with the polypeptide shown in SEQ ID NO: 53. q) A polypeptide having at least 98% sequence identity with the polypeptide shown in SEQ ID NO: 56. r) A polypeptide having at least 94% sequence identity with the polypeptide shown in SEQ ID NO: 59. s) A polypeptide having at least 85% sequence identity with the polypeptide shown in SEQ ID NO: 62. t) A polypeptide having at least 97% sequence identity with the polypeptide shown in SEQ ID NO: 68. u) A polypeptide having at least 99% sequence identity with the polypeptide shown in SEQ ID NO: 71. v) A polypeptide having at least 91% sequence identity with the polypeptide shown in SEQ ID NO: 74. w) A polypeptide having at least 98% sequence identity with the polypeptide shown in SEQ ID NO: 77. x) A polypeptide having at least 81% sequence identity with the polypeptide shown in SEQ ID NO: 83. y) A polypeptide having at least 89% sequence identity with the polypeptide shown in SEQ ID NO: 86. z) A polypeptide having at least 99% sequence identity with the polypeptide shown in SEQ ID NO: 89. aa) A polypeptide having at least 91% sequence identity with the polypeptide shown in SEQ ID NO: 92. (bb) A polypeptide having at least 90% sequence identity with the polypeptide shown in SEQ ID NO: 95. (cc) A polypeptide having at least 91% sequence identity with the polypeptide shown in SEQ ID NO: 98. (dd) A polypeptide having at least 92% sequence identity with the polypeptide shown in SEQ ID NO: 104. (ee) A polypeptide having at least 90% sequence identity with the polypeptide shown in SEQ ID NO: 107.

[0458] (ff) A polypeptide having at least 91.5% sequence identity with the polypeptide shown in SEQ ID NO: 110.

[0459] (gg) A polypeptide having at least 93% sequence identity with the polypeptide shown in SEQ ID NO: 113. (hh) A polypeptide having at least 96% sequence identity with the polypeptide shown in SEQ ID NO: 116. ii) A polypeptide having at least 99.5% sequence identity with the polypeptide shown in SEQ ID NO: 119. (jj) A polypeptide having at least 99.5% sequence identity with the polypeptide shown in SEQ ID NO: 128. (kk) is a polypeptide that has at least 93% sequence identity with the polypeptide shown in SEQ ID NO: 131. ll) A polypeptide having at least 79% sequence identity with the polypeptide shown in SEQ ID NO: 134. A polypeptide having at least 72% sequence identity with the polypeptide shown in SEQ ID NO: 137 (mm). (nn) A polypeptide having at least 77% sequence identity with the polypeptide shown in SEQ ID NO: 140, (oo) A polypeptide having at least 74% sequence identity with the polypeptide shown in SEQ ID NO: 143, (pp) A polypeptide having at least 97% sequence identity with the polypeptide shown in SEQ ID NO: 146. (qq) A polypeptide having at least 71% sequence identity with the polypeptide shown in SEQ ID NO: 149. (rr) A polypeptide having at least 96% sequence identity with the polypeptide shown in SEQ ID NO: 152. ss) a polypeptide having at least 72% sequence identity with the polypeptide shown in SEQ ID NO: 155, (tt) A polypeptide having at least 93% sequence identity with the polypeptide shown in SEQ ID NO: 158. uu) is a polypeptide that has at least 85% sequence identity with the polypeptide shown in SEQ ID NO: 164. (vv) A polypeptide having at least 88% sequence identity with the polypeptide shown in SEQ ID NO: 167 The polypeptide having at least 99.8% sequence identity with the polypeptide shown in SEQ ID NO: 170. (xx) A polypeptide having at least 97% sequence identity with the polypeptide shown in SEQ ID NO: 173. (yy) A polypeptide having at least 87% sequence identity with the polypeptide shown in SEQ ID NO: 176 (zz) A polypeptide having at least 81% sequence identity with the polypeptide shown in SEQ ID NO: 179. aaa) is a polypeptide that has at least 89% sequence identity with the polypeptide shown in SEQ ID NO: 182. (bbb) A polypeptide having at least 96% sequence identity with the polypeptide shown in SEQ ID NO: 185. (ccc) A polypeptide having at least 88% sequence identity with the polypeptide shown in SEQ ID NO: 188. (ddd) A polypeptide having at least 87% sequence identity with the polypeptide shown in SEQ ID NO: 191. eee) is a polypeptide that has at least 94% sequence identity with the polypeptide shown in SEQ ID NO: 194. fff) A polypeptide having at least 90% sequence identity with the polypeptide shown in SEQ ID NO: 197, and optionally ggg) One or more of the motifs [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V] or C[D / N]T[A / R] and [D / Q][I / V]DH.

[0460] In one aspect of the invention, the DNase is obtained from a species of the genus Bacillus. 62451 Bacillus Horikoshi or species of the genus Bacillus- 18057 The DNase of the present invention comprises a mature polypeptide of SEQ ID NO: 2, 4 or 6 or a polypeptide having at least 60%, for example at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with the mature polypeptide of SEQ ID NO: 2, 4 or 6, and wherein such polypeptides have DNase activity.

[0461] The DNase groups contained in the GYS-clade, as described above, share similar structural and functional characteristics, such as common motifs. The DNases of the GYS-clade are preferably obtained from the genus *Bacillus*. Individual DNases within the GYS group are described in detail below.

[0462] DNA enzymes can be obtained from Bacillus species, with a preference for Bacillus species. 62451 The DNase can be a polypeptide containing the mature polypeptide of SEQ ID NO 2 or a polypeptide closely related to it, for example, a polypeptide having at least 60%, for example, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, or for example, at least 95% sequence identity with it. The DNase according to the invention can be obtained from the genus Bacillus, for example, species of the genus Bacillus- 62451And comprising a polypeptide having at least 60%, such as at least 65%, at least 70%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 8. Containing SEQ ID NO 21 (a mature polypeptide obtained from Bacillus foodae) and SEQ ID NO 22 (a polypeptide obtained from Bacillus species- 18318 The polypeptides of SEQ ID NO 23 (mature polypeptide obtained from Bacillus subtilis) and SEQ ID NO 23 (mature polypeptide obtained from Bacillus subtilis) are, for example, homologous polypeptides having at least 80% sequence identity with SEQ ID NO 8.

[0463] Contains SEQ ID NO 21 (mature polypeptide obtained from Bacillus foodae) and SEQ ID NO 22 (obtained from Bacillus species- 18318 The polypeptides of SEQ ID NO 21 (mature polypeptide) and SEQ ID NO 23 (mature polypeptide obtained from Bacillus cereus) can also be used to prevent or remove biofilms on articles such as textiles and / or fabrics as shown in Example 2. The DNase can be a polypeptide comprising the mature polypeptide shown in SEQ ID NO 21 or a polypeptide closely related thereto. Therefore, one aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 21, preferably obtained from Bacillus cereus. 。The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 22. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 22, preferably obtained from a Bacillus species. 18318 The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 23. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 23, preferably obtained from Bacillus subtilis.

[0464] The DNase can be obtained from the genus Bacillus, preferably Bacillus Horikoshi. The DNase can be a polypeptide containing the mature polypeptide of SEQ ID NO4 or a polypeptide closely related to it, for example, a polypeptide having at least 60%, for example, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, or for example, at least 95% sequence identity with it. The DNase according to the invention can be obtained from the genus Bacillus, such as Bacillus Horikoshi, and comprises a polypeptide having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 9. SEQ ID NO 11 (obtained from Bacillus species- 62520Mature polypeptide), SEQ ID NO 12 (obtained from Bacillus species- 62520 Mature polypeptides), SEQ ID NO 13 (mature polypeptide obtained from Bacillus Horikoshii), SEQ ID NO 14 (mature polypeptide obtained from Bacillus Horikoshii), SEQ ID NO 15 (mature polypeptide obtained from Bacillus species- 16840 Mature polypeptide), SEQ ID NO 16 (obtained from Bacillus species- 16840 Mature polypeptide), SEQ ID NO 17 (obtained from Bacillus species- 62668 Mature polypeptide), SEQ ID NO 18 (obtained from Bacillus species- 13395 Mature polypeptide), SEQ ID NO 19 (mature polypeptide obtained from Bacillus honekianis) or SEQ ID NO 20 (mature polypeptide obtained from Bacillus species- 11238 The homologous polypeptide contained in the mature polypeptide is a homologous polypeptide with at least 80% sequence identity to SEQ ID NO 9. It contains SEQ ID NO 11 (obtained from Bacillus species- 62520 Mature polypeptide), SEQ ID NO 12 (obtained from Bacillus species- 62520 Mature polypeptides), SEQ ID NO 13 (mature polypeptide obtained from Bacillus Horikoshii), SEQ ID NO 14 (mature polypeptide obtained from Bacillus Horikoshii), SEQ ID NO 15 (mature polypeptide obtained from Bacillus species- 16840 Mature polypeptide), SEQ ID NO 16 (obtained from Bacillus species- 16840 Mature polypeptide), SEQ ID NO 17 (obtained from Bacillus species- 62668 Mature polypeptide), SEQ ID NO 18 (obtained from Bacillus species- 13395 Mature polypeptide), SEQ ID NO 19 (mature polypeptide obtained from Bacillus honekianis) or SEQ ID NO 20 (mature polypeptide obtained from Bacillus species- 11238The mature polypeptide (of which the polypeptide is present) can also be used to prevent or remove biofilms on articles such as textiles and / or fabrics as shown in Example 2. The DNase can be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 11. Therefore, one aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 11, preferably obtained from a Bacillus species. 62520 The DNase can be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 12. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 12, preferably obtained from Bacillus species. 62520The DNase may be a polypeptide comprising the mature polypeptide shown in SEQ ID NO 13 or a polypeptide closely related thereto. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 13, preferably obtained from *Bacillus Horikoshii*. The DNase may be a polypeptide comprising the mature polypeptide shown in SEQ ID NO 14 or a polypeptide closely related thereto. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 14, preferably obtained from Bacillus Horikoshii. The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 15. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 15, preferably obtained from a Bacillus species. 16840The DNase can be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 16. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 16, preferably obtained from Bacillus species. 16840 The DNase can be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 17. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 17, preferably obtained from Bacillus species. 62668 The DNase can be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 18. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 18, preferably obtained from Bacillus species. 13395The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 19. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 19, preferably obtained from Bacillus thunbergii.

[0465] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 20. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 20, preferably obtained from Bacillus species. 11238 .

[0466] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 53. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 53, preferably obtained from Bacillus oryzae.

[0467] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 56. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with xanthan gum communities, preferably obtained from xanthan gum communities. J .

[0468] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 59. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with Bacillus viviparus, preferably obtained from Bacillus vivax.

[0469] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 62. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 62, preferably obtained from Bacillus hanamida.

[0470] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 68. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 68, preferably obtained from Bacillus indicus.

[0471] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 71. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 71, preferably obtained from Bacillus thuringiensis.

[0472] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 74. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 74, preferably obtained from Bacillus Lucifer.

[0473] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 77. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 77, preferably obtained from Bacillus thuringiensis.

[0474] The DNase groups contained in the NAWK clade, as described above, share similar structural and functional characteristics, such as common motifs. DNases of the NAWK clade can be obtained from any of the genera and species listed below. Individual DNases within the NAWK group are described in detail below.

[0475] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 83. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 83, preferably obtained from *Echinochloa* species.

[0476] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 86. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 86, preferably obtained from… Vibrissea flavovirens .

[0477] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 89. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 89, preferably obtained from… Setosphaeria rostrata .

[0478] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 92. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 92, preferably obtained from… Endophragmiella valdina .

[0479] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 95. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 95, preferably obtained from *Corynebacterium multiflorum*.

[0480] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 98. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 98, preferably obtained from species of the genus *Heterostigma*. XZ1965 .

[0481] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 101. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with *Prunus persica*, preferably obtained from *Prunus persica*.

[0482] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 104. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 104, preferably obtained from Curvularia crescentis.

[0483] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 107. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 107, preferably obtained from *Penicillium spp.*

[0484] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 110. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 110, preferably obtained from… Penicillium quercetorum .

[0485] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 113. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 113, preferably obtained from… Setophaeosphaeria Species.

[0486] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 116. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 116, preferably obtained from Alternaria species. XZ2545 .

[0487] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 119. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 119, preferably obtained from Alternaria species.

[0488] The DNase groups contained in the KNAW clade, as described above, share similar structural and functional characteristics, such as common motifs. DNases of the NAWK clade are preferably obtained from any of the genera and species listed below. Individual DNases in the NAWK group are described in detail below.

[0489] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 128. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 128, preferably obtained from thermophilic chromophores.

[0490] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 131. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 131, preferably obtained from… Metapochonia suchlasporia .

[0491] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 134. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 134, preferably obtained from *Cyclocarya paliurus*.

[0492] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 137. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 137, preferably obtained from species of the genus *Cladosporium*. XZ2007 .

[0493] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 140. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with *Acer dichromatosa*, preferably obtained from *Acer dichromatosa*.

[0494] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 143. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 143, preferably obtained from species of the genus *Pleurotus*. XZ2014 .

[0495] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 146. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 146, preferably obtained from species of the genus *Metarhizium*. HNA15-2 .

[0496] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 149. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 149, preferably obtained from species of the genus *Cladosporium*. XZ2414 .

[0497] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 152. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 152, preferably obtained from *Cladosporium spp.*

[0498] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 155. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 155, preferably obtained from *Arthropoda spiralis*.

[0499] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 158. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 158, preferably obtained from Metarhizium anisopliae.

[0500] The DNase-active peptides listed below can also be used for deep cleaning, for example, to prevent, reduce, or remove biofilms on fabrics such as textiles (e.g., cotton and polyester). The DNase-active peptides listed below contain NUC1 and NUC1_A domains and NUC1 and NUC1_A motifs, and are similar to peptides belonging to any of the clades GYS, NAWK, and KNAW, which also contain NUC1 and NUC1_A domains and motifs.

[0501] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 164. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 164, preferably obtained from… Sporormia fimetaria .

[0502] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 167. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 167, preferably obtained from… Pycnidiophora cf. dispera .

[0503] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 170. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with xanthan gum basic communities, preferably obtained from xanthan gum basic communities. D .

[0504] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 173. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with xanthan gum basic community O, preferably obtained from xanthan gum basic community O.

[0505] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 176. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 176, preferably obtained from species of the Clavicipitaceae family. 70249 .

[0506] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 179. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 179, preferably obtained from species of the genus *Westergasse*. AS85-2 .

[0507] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 182. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 182, preferably obtained from… Humicolopsis cephalosporioides .

[0508] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 185. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 185, preferably obtained from… Neosartorya massa .

[0509] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 188. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 188, preferably obtained from… Roussoella intermedia .

[0510] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 191. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 191, preferably obtained from the order Pleuronectiformes.

[0511] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 194. One aspect of the invention relates to a DNase having at least 60%, for example at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 194, preferably obtained from species of the genus *Cyclocarya*.

[0512] The DNase may be a polypeptide comprising or closely related to the mature polypeptide shown in SEQ ID NO 197. One aspect of the invention relates to a DNase having at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 197, preferably obtained from… Didymosphaeria futilis .

[0513] DNase can be obtained from Bacillus species, with a preference for Bacillus species. 18057 The DNase can be a polypeptide containing the mature polypeptide of SEQ ID NO 6 or a polypeptide closely related thereto. The DNase according to the present invention can be obtained from Bacillus species- 18057 And contains a polypeptide that has at least 60%, such as at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, or 100% sequence identity with SEQ ID NO 10.

[0514] In one aspect of the invention, the polypeptide with DNase activity is obtained from Bacillus species, specifically from Bacillus species - 62451 In one aspect of the invention, the polypeptide with DNase activity is obtained from the genus *Bacillus*, specifically from *Bacillus Horikoshi*. In another aspect of the invention, the polypeptide with DNase activity is obtained from the genus *Bacillus*, specifically from species of the genus *Bacillus*. 62520 In one aspect of the invention, the polypeptide with DNase activity is obtained from Bacillus species, specifically from Bacillus species - 16840 In one aspect of the invention, the polypeptide with DNase activity is obtained from Bacillus species, specifically from Bacillus species - 62668In one aspect of the invention, the polypeptide with DNase activity is obtained from Bacillus species, specifically from Bacillus species - 13395 In one aspect of the invention, the polypeptide with DNase activity is obtained from Bacillus species, specifically from Bacillus species - 11238 In one aspect of the invention, the polypeptide with DNase activity is obtained from the genus *Bacillus*, specifically from *Bacillus oryzae*. In another aspect of the invention, the polypeptide with DNase activity is obtained from the genus *Bacillus*, specifically from species of the genus *Bacillus*. 18318 In one aspect of the invention, the polypeptide with DNase activity is obtained from Bacillus, specifically from Bacillus strains from the Institute of Pathogenic Biology. In one aspect of the invention, the polypeptide with DNase activity is obtained from... Vibrissea flavovirens The polypeptide, and comprises or is composed of the polypeptide shown in SEQ ID NO 86, is also present in one aspect of the invention. In another aspect, the DNase-active polypeptide is obtained from *Acer dichromatosa* and comprises or is composed of the polypeptide shown in SEQ ID NO 140. In yet another aspect, the DNase-active polypeptide is obtained from a species of the Clavicipitaceae family. 70249 The polypeptide, having DNase activity, is obtained from *Penicillium spp.* and comprises or is composed of the polypeptide shown in SEQ ID NO 176. In one aspect of the invention, the polypeptide having DNase activity is obtained from... Pycnidiophora cf. dispera And contains or constitutes the polypeptide shown in SEQ ID NO 167. In one aspect of the invention, the polypeptide having DNase activity is obtained from... Metapochonia suchlasporia And contains or constitutes the polypeptide shown in SEQ ID NO 131. In one aspect of the invention, the polypeptide having DNase activity is obtained from a species of the genus *Cladosporium*. XZ2007 And contains or constitutes the polypeptide shown in SEQ ID NO 137. In one aspect of the invention, the polypeptide having DNase activity is obtained from... Setosphaeria rostrata And it contains or is composed of the polypeptide shown in SEQ ID NO 89. In one aspect of the invention, the polypeptide having DNase activity is obtained from a species of the genus *Pleurotus*. XZ2014 And it comprises or is composed of the polypeptide shown in SEQ ID NO 143. In one aspect of the invention, the polypeptide having DNase activity is obtained from a species of the genus *Metarhizium*. HNA15-2 And contains or constitutes the polypeptide shown in SEQ ID NO 146. In one aspect of the invention, the polypeptide having DNase activity is obtained from... Endophragmiella valdina And contains or constitutes the polypeptide shown in SEQ ID NO 92. In one aspect of the invention, the polypeptide having DNase activity is obtained from... Humicolopsis cephalosporioides The polypeptide, having DNase activity, is obtained from *Hypertriciscus* species and comprises or is composed of the polypeptide shown in SEQ ID NO 182. In one aspect of the invention, the polypeptide having DNase activity is obtained from *Hypertriciscus* species. XZ1965 The polypeptide, having DNase activity, is obtained from *Curvularia crescentis* and comprises or is composed of the polypeptide shown in SEQ ID NO 98. In one aspect of the invention, the polypeptide having DNase activity is obtained from a species of the genus *Curvularia*. XZ2414 The polypeptide, having DNase activity, is obtained from *Clematis chinensis* and comprises or is composed of the polypeptide shown in SEQ ID NO 149. In one aspect of the invention, the polypeptide having DNase activity is obtained from... Roussoella intermedia And it comprises or is composed of the polypeptide shown in SEQ ID NO 188. In one aspect of the invention, the polypeptide having DNase activity is obtained from *Arthropoda spiralis*, and comprises or is composed of the polypeptide shown in SEQ ID NO 155. In one aspect of the invention, the polypeptide having DNase activity is obtained from... Setophaeosphaeria The species, and comprising or consisting of the polypeptide shown in SEQ ID NO 158. In one aspect of the invention, the polypeptide having DNase activity is obtained from a species of the genus Alternaria. XZ2545 The polypeptide, having DNase activity, is obtained from a species of the genus *Alternaria* and comprises or is composed of the polypeptide shown in SEQ ID NO 116. In one aspect of the invention, the polypeptide having DNase activity is obtained from *Metarhizium anisopliae* and comprises or is composed of the polypeptide shown in SEQ ID NO 158. In one aspect of the invention, the polypeptide having DNase activity is obtained from the order *Plasmodium* and comprises or is composed of the polypeptide shown in SEQ ID NO 191. In one aspect of the invention, the polypeptide having DNase activity is obtained from a species of the genus *Cyclophorus* and comprises or is composed of the polypeptide shown in SEQ ID NO 194. In one aspect of the invention, the polypeptide having DNase activity is obtained from... Didymosphaeria futilisThe polypeptide, having DNase activity, is obtained from *Bacillus viminalis* and comprises or is composed of the polypeptide shown in SEQ ID NO 197. In one aspect of the invention, the polypeptide having DNase activity is obtained from *Bacillus hanajinda* and comprises or is composed of the polypeptide shown in SEQ ID NO 62. In one aspect of the invention, the polypeptide having DNase activity is obtained from xanthan gum basic communities. J The polypeptide, having DNase activity, is obtained from *Bacillus indicus* and comprises or is composed of the polypeptide shown in SEQ ID NO 56. In one aspect of the invention, the polypeptide having DNase activity is obtained from *Bacillus indicus* and comprises or is composed of the polypeptide shown in SEQ ID NO 68. In one aspect of the invention, the polypeptide having DNase activity is obtained from *Bacillus lutea* and comprises or is composed of the polypeptide shown in SEQ ID NO 71. In one aspect of the invention, the polypeptide having DNase activity is obtained from *Bacillus lucifera* and comprises or is composed of the polypeptide shown in SEQ ID NO 74. In one aspect of the invention, the polypeptide having DNase activity is obtained from *Bacillus lutea* and comprises or is composed of the polypeptide shown in SEQ ID NO 77. In one aspect of the invention, the polypeptide having DNase activity is obtained from... Sporormia fimetaria The polypeptide, having DNase activity, is obtained from *Cyclocarya paliurus* species and comprises or is composed of the polypeptide shown in SEQ ID NO 164. In one aspect of the invention, the polypeptide having DNase activity is obtained from *Cyclocarya paliurus* species and comprises or is composed of the polypeptide shown in SEQ ID NO 83. In one aspect of the invention, the polypeptide having DNase activity is obtained from *Cyclocarya paliurus* species. AS85-2 The polypeptide, having DNase activity, is obtained from *Prunus cerevisiae*, and comprises or is composed of the polypeptide shown in SEQ ID NO 179. In one aspect of the invention, the polypeptide having DNase activity is obtained from... Neosartorya massa And contains or constitutes the polypeptide shown in SEQ ID NO 185. In one aspect of the invention, the polypeptide having DNase activity is obtained from... Penicillium quercetorum And it comprises or is composed of the polypeptide shown in SEQ ID NO 110. In one aspect of the invention, the polypeptide having DNase activity is obtained from xanthan gum basic communities. DThe polypeptide, and comprises or is composed of the polypeptide shown in SEQ ID NO 170, is also present in one aspect of the invention. In another aspect, the DNase-active polypeptide is obtained from *Bacillus alginate* and comprises or is composed of the polypeptide shown in SEQ ID NO 53. In yet another aspect, the DNase-active polypeptide is obtained from xanthan gum alkaline communities. O The polypeptide contains or is composed of the polypeptide shown in SEQ ID NO 173. In one aspect of the invention, the polypeptide having DNase activity is obtained from *Lysimachia thermophila* and contains or is composed of the polypeptide shown in SEQ ID NO 128.

[0515] In one aspect of the invention, the polypeptide with DNase activity is obtained from the genus *Bacillus*, specifically from a species of the genus *Bacillus*. 18057 In one aspect of the invention, the polypeptide with DNase activity is obtained from the genus *Bacillus* and comprises a mature polypeptide of SEQ ID NO 2, 4, or 6, i.e., a mature polypeptide of SEQ ID NO 8, 9, or 10. In a preferred aspect of the invention, the DNase is obtained from a species of the genus *Bacillus*. 62451 The DNase contains a polypeptide sequence having SEQ ID NO 8. In a preferred aspect of the invention, the DNase is obtained from *Bacillus Horikoshii* and contains a polypeptide sequence having SEQ ID NO 9. In a preferred aspect of the invention, the DNase is obtained from a species of the genus *Bacillus*. 18057 And contains any one of the polypeptide sequences having SEQ ID NO 10. In a preferred aspect of the invention, the DNase is obtained from a Bacillus species- 62520 And it contains a polypeptide sequence having SEQ ID NO 11. In a preferred aspect of the invention, the DNase is obtained from a Bacillus species- 62520 The DNase contains a polypeptide sequence having SEQ ID NO 12. In a preferred aspect of the invention, the DNase is obtained from *Bacillus Horikoshii* and contains a polypeptide sequence having SEQ ID NO 13. In a preferred aspect of the invention, the DNase is obtained from *Bacillus Horikoshii* and contains a polypeptide sequence having SEQ ID NO 14. In a preferred aspect of the invention, the DNase is obtained from a species of the genus *Bacillus*. 16840 And it contains a polypeptide sequence having SEQ ID NO 15. In a preferred aspect of the invention, the DNase is obtained from a Bacillus species- 16840 And it contains a polypeptide sequence having SEQ ID NO 16. In a preferred aspect of the invention, the DNase is obtained from a Bacillus species- 62668And it contains a polypeptide sequence having SEQ ID NO 17. In a preferred aspect of the invention, the DNase is obtained from a species of the genus Bacillus. 13395 The DNAase contains a polypeptide sequence having SEQ ID NO 18. In a preferred aspect of the invention, the DNAase is obtained from *Bacillus honekianis* and contains a polypeptide sequence having SEQ ID NO 19. In a preferred aspect of the invention, the DNAase is obtained from a species of the genus *Bacillus*. 11238 And contains a polypeptide sequence having SEQ ID NO 20. In a preferred aspect of the invention, the DNase is obtained from food Bacillus and contains a polypeptide sequence having SEQ ID NO 21. In a preferred aspect of the invention, the DNase is obtained from a species of the genus Bacillus. 18318 The DNAse is derived from Bacillus subtilis and contains a polypeptide sequence having SEQ ID NO 22. In a preferred aspect of the invention, the DNAse is obtained from Bacillus subtilis and contains a polypeptide sequence having SEQ ID NO 23.

[0516] In a preferred aspect of the invention, the DNase is obtained from a species of the genus Bacillus. 62451 And it consists of a polypeptide sequence having SEQ ID NO 8. In another preferred aspect of the invention, the DNase is obtained from Bacillus Horikoshii and consists of a polypeptide sequence having SEQ ID NO 9. In another preferred aspect of the invention, the DNase is obtained from a species of the genus Bacillus- 18057 And it consists of a polypeptide sequence having SEQ ID NO 10. In a preferred aspect of the invention, the DNase is obtained from a species of the genus Bacillus. 62520 And it consists of a polypeptide sequence having SEQ ID NO 11. In a preferred aspect of the invention, the DNase is obtained from a species of the genus Bacillus. 62520 The DNase is derived from *Bacillus horikosa* and consists of a polypeptide sequence having SEQ ID NO 12. In a preferred aspect of the invention, the DNase is obtained from *Bacillus horikosa* and consists of a polypeptide sequence having SEQ ID NO 13. In a preferred aspect of the invention, the DNase is obtained from *Bacillus* species... 16840 And it consists of a polypeptide sequence having SEQ ID NO 15. In a preferred aspect of the invention, the DNase is obtained from a species of the genus Bacillus. 16840 And it consists of a polypeptide sequence having SEQ ID NO 16. In a preferred aspect of the invention, the DNase is obtained from a species of the genus Bacillus. 62668And it consists of a polypeptide sequence having SEQ ID NO 17. In a preferred aspect of the invention, the DNase is obtained from a species of the genus Bacillus. 13395 And it consists of a polypeptide sequence having SEQ ID NO 18. In a preferred aspect of the invention, the DNase is obtained from Bacillus honekianis and consists of a polypeptide sequence having SEQ ID NO 19. In a preferred aspect of the invention, the DNase is obtained from a species of Bacillus. 11238 And it consists of a polypeptide sequence having SEQ ID NO 20. In a preferred aspect of the invention, the DNase is obtained from food-bacterial Bacillus and consists of a polypeptide sequence having SEQ ID NO 21. In a preferred aspect of the invention, the DNase is obtained from a species of the genus Bacillus- 18318 Furthermore, it comprises a polypeptide sequence having SEQ ID NO 22. In a preferred aspect of the invention, the DNase is obtained from Bacillus subtilis and comprises a polypeptide sequence having SEQ ID NO 23.

[0517] Biofilms form on textiles when microorganisms are present on the surface and adhere to it. Some microorganisms tend to adhere to the surface of items such as textiles. Some microorganisms adhere to such surfaces and form biofilms. Biofilms can be sticky, and the adhered microorganisms and / or biofilms can be difficult to remove. Furthermore, due to the sticky nature of biofilms, they adhere to dirt. Commercially available laundry detergent compositions do not remove such adhered microorganisms, microbial fragments, or biofilms.

[0518] This invention relates to polypeptides with DNase activity and the use of such polypeptides to prevent, reduce, or remove biofilms from articles such as textiles. In one embodiment of the invention, the polypeptide with DNase activity is used to prevent, reduce, or remove the stickiness of articles. In one embodiment of the invention, the polypeptide with DNase activity improves the whiteness of articles such as textiles. In one embodiment, the polypeptide of the invention with DNase activity helps maintain the color on textiles. When textiles are washed repeatedly, the color tends to become less bright. In one embodiment, the polypeptide of the invention with DNase activity has an improving effect on maintaining the color of colored textiles, even after repeated washing. In one embodiment, the polypeptide of the invention can also reduce the staining of non-colored portions of the same or other textiles present during washing.

[0519] Peptides with DNase activity can be further used to pretreat stains on textiles, such as textiles with a significant amount of biofilm adhering to them.

[0520] This DNase-active polypeptide can be further used to prevent, reduce, or remove static electricity from items that accumulate static electricity, such as textiles or hard surfaces. It can also be used to prevent, reduce, and / or remove biofilms from items that are hard surfaces, such as tableware, knives, porcelain, ceramics, pottery, etc. Therefore, in some respects, this DNase-active polypeptide can be used in ADW (Automatic Dishwashing) processes.

[0521] Furthermore, this invention relates to the use of polypeptides with DNase activity in preventing, reducing, or removing dirt redeposition during a washing cycle. When this polypeptide is used, for example, in the washing of textiles, it prevents dirt present in the washing liquid from depositing on the textiles.

[0522] Furthermore, the present invention relates to the use of polypeptides with DNase activity for preventing, reducing, or removing the adhesion of dirt to articles. In one embodiment, the article is a textile. When the dirt is not adhered to the article, the article appears cleaner. Therefore, the present invention further relates to the use of polypeptides with DNase activity for maintaining or improving the whiteness of the article.

[0523] When items (like T-shirts or sportswear) are used, they are exposed to bacteria from the user's body and the rest of the environment in which they are used. This can cause unpleasant odors on the items, even after washing. This invention relates to the removal or reduction of unpleasant odors on textiles. These odors may be caused by bacteria that produce compounds with unpleasant smells. One example of such compounds with unpleasant odors is E-2-nonenal. The odor can be present on freshly washed textiles that are still damp, or it can be present on freshly washed textiles that are essentially dry. The odor can also be present on textiles that have been stored for a period of time after washing. This invention relates to reducing or removing unpleasant odors such as E-2-nonenal from wet or dry textiles.

[0524] The polypeptides with DNase activity (i.e., the DNase of the present invention) have very good cleaning properties in powder and liquid detergents. Examples of beneficial effects of DNases and homologous DNases having SEQ ID NO 8, 9, and 10, such as polypeptides having DNase activity and having the polypeptide sequences shown in the following items: SEQ ID NO 11, SEQ ID NO 12, SEQ ID NO 13, SEQ ID NO 14, SEQ ID NO 15, SEQ ID NO 16, SEQ ID NO 17, SEQ ID NO 18, SEQ ID NO 19, SEQ ID NO 20, SEQ ID NO 21, SEQ ID NO 22, SEQ ID NO 23, SEQ ID NO 53, SEQ ID NO 56, SEQ ID NO 59, SEQ ID NO 62, SEQ ID NO 65, SEQ ID NO 68, SEQ ID NO 71, SEQ ID NO 74, SEQ ID NO 77, SEQ ID NO 80, SEQ ID NO 83, SEQ ID NO 86, SEQ ID NO 89, SEQ ID NO 92, SEQ ID NO 95, SEQ ID NO 98, SEQ ID NO 101. SEQ ID NO 104, SEQ ID NO 107, SEQ ID NO 110, SEQ ID NO 113, SEQ ID NO 116, SEQ ID NO 119, SEQ ID NO 122, SEQ ID NO 125, SEQ ID NO 128, SEQ ID NO 131, SEQ ID NO 134, SEQ ID NO 137, SEQ ID NO 140. SEQ ID NO 143, SEQ ID NO 146, SEQ ID NO 149, SEQ ID NO 152, SEQ ID NO 155, SEQ ID NO 158, SEQ ID NO 161, SEQ ID NO 164, SEQ ID NO 167, SEQ ID NO 170, SEQ ID NO 173, SEQ ID NO 176, SEQ ID NO 179, SEQ ID NO 182, SEQ ID NO 185, SEQ ID NO 188, SEQ ID NO 191, SEQ ID NO 194 and SEQ ID NO 197. The deep cleaning effect is shown in Examples 2 and 3, one effect being the prevention of clothes from turning gray and the removal of odors.The peptides containing SEQ ID NO 8, 9 and 10 are novel peptides with DNase activity, which have a deep cleaning effect in powder detergents and liquid detergents. Contains SEQ ID NO 11, SEQ ID NO 12, SEQ ID NO 13, SEQ ID NO 14, SEQ ID NO 15, SEQ ID NO 16, SEQ ID NO 17, SEQ ID NO 18, SEQ ID NO 19, SEQ ID NO 20, SEQ ID NO 21, SEQ ID NO 22, SEQ ID NO 23, SEQ ID NO 53, SEQ ID NO 56. SEQ ID NO 59, SEQ ID NO 62, SEQ ID NO 68, SEQ ID NO 71, SEQ ID NO 74, SEQ ID NO 77, SEQ ID NO 80, SEQ ID NO 83, SEQ ID NO 86, SEQ ID NO 89, SEQ ID NO 92, SEQ ID NO 95, SEQ ID NO 98, SEQ ID NO 104, SEQ ID NO 107, SEQ ID NO 110, SEQ ID NO 113, SEQ ID NO 116, SEQ ID NO 119, SEQ ID NO The peptides 122, SEQ ID NO 125, SEQ ID NO 128, SEQ ID NO 131, SEQ ID NO 134, SEQ ID NO 137, SEQ ID NO 140, SEQ ID NO 143, SEQ ID NO 146, SEQ ID NO 149, SEQ ID NO 152, SEQ ID NO 158, SEQ ID NO 161, SEQ ID NO 164, SEQ ID NO 167, SEQ ID NO 170, SEQ ID NO 176, SEQ ID NO 179, SEQ ID NO 185, SEQ ID NO 188, SEQ ID NO 191, and SEQ ID NO 197 are also peptides with DNase activity, and these peptides have a deep cleaning effect in powder detergents and liquid detergents.

[0525] Benzonase (SIGMA-E1014) (SEQ ID NO 7) is a commercially available DNase. The inventors state that this DNase also exhibits deep cleaning effects, as seen in Example 2. Deep cleaning helps prevent dust accumulation on clothing and removes odors.

[0526] Another embodiment relates to the use of DNases selected from the group consisting of SEQ ID NO 7, 8, 9 and 10 for reducing odors from clothing and / or textiles. Another embodiment relates to the use of DNases selected from the group consisting of SEQ ID NO 7, 8, 9 and 10 for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0527] Some aspects of the present invention relate to the use of a DNase-active polypeptide for preventing, reducing, or removing biofilms from an article, wherein the DNase-active polypeptide comprises an HXXP motif, and wherein H is the amino acid histidine, P is the amino acid proline, and X is any amino acid. The article is preferably a fabric, such as textiles, for example cotton and / or polyester.

[0528] Some aspects of the present invention relate to the use of polypeptides having DNase activity for preventing, reducing, or removing biofilms from articles, wherein the polypeptide having DNase activity comprises one or more of the motifs [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L]. The article is preferably a fabric, such as textiles, for example cotton and / or polyester.

[0529] Some aspects of the present invention relate to the use of polypeptides having DNase activity for preventing, reducing, or removing biofilms from articles, wherein the polypeptide having DNase activity comprises one or both of the motifs [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R]. The article is preferably a fabric, such as textiles, for example cotton and / or polyester.

[0530] Some aspects of the present invention relate to the use of a DNase-active polypeptide for preventing, reducing, or removing biofilms from an article, wherein the DNase-active polypeptide belongs to the GYS clade and contains one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205). The article is preferably a fabric, such as textiles, like cotton and / or polyester.

[0531] Some aspects of the present invention relate to the use of DNase-active polypeptides for preventing, reducing, or removing biofilms from articles, wherein the DNase-active polypeptide belongs to the GYS clade and comprises polypeptides selected from SEQ ID NO 8, SEQ ID NO 9, SEQ ID NO 11, SEQ ID NO 12, SEQ ID NO 13, SEQ ID NO 14, SEQ ID NO 15, SEQ ID NO 16, SEQ ID NO 17, SEQ ID NO 18, SEQ ID NO 19, SEQ ID NO 20, SEQ ID NO 21, SEQ ID NO 22, SEQ ID NO 23, SEQ ID NO 53, SEQ ID NO 56, SEQ ID NO 59, SEQ ID NO 62, SEQ ID NO 65, SEQ ID NO 68, SEQ ID NO 71, SEQ ID NO 74, SEQ ID NO 77 and SEQ ID NO The article is a polypeptide shown in 80 or a polypeptide having at least 80%, for example, at least 85%, for example, at least 90%, for example, at least 95%, or 100% sequence identical. The article is preferably a fabric, such as textiles, such as cotton and / or polyester.

[0532] Some aspects of the present invention relate to the use of a DNase-active polypeptide for preventing, reducing, or removing biofilms from an article, wherein the DNase-active polypeptide belongs to the NAWK clade and contains one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 206) or NPQL (SEQ ID NO: 207). The article is preferably a fabric, such as textiles, like cotton and / or polyester.

[0533] Some aspects of the present invention relate to the use of DNase-active polypeptides for preventing, reducing, or removing biofilms from articles, wherein the DNase-active polypeptide belongs to the NAWK clade and comprises polypeptides selected from those shown in SEQ ID NO 83, SEQ ID NO 86, SEQ ID NO 89, SEQ ID NO 92, SEQ ID NO 95, SEQ ID NO 98, SEQ ID NO 101, SEQ ID NO 104, SEQ ID NO 107, SEQ ID NO 110, SEQ ID NO 113, SEQ ID NO 116, and SEQ ID NO 119, or polypeptides having at least 80%, for example, at least 85%, for example, at least 90%, for example, at least 95%, or 100% sequence identity with them. The article is preferably a fabric, such as textiles, such as cotton and / or polyester.

[0534] Some aspects of the present invention relate to the use of a DNase-active polypeptide for preventing, reducing, or removing biofilms from an article, wherein the DNase-active polypeptide belongs to the KNAW clade and contains one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209). The article is preferably a fabric, such as textiles, like cotton and / or polyester.

[0535] Some aspects of the present invention relate to the use of DNase-active polypeptides for preventing, reducing, or removing biofilms from articles, wherein the DNase-active polypeptide belongs to the KNAW clade and comprises polypeptides selected from those shown in SEQ ID NO 122, SEQ ID NO 125, SEQ ID NO 128, SEQ ID NO 131, SEQ ID NO 134, SEQ ID NO 137, SEQ ID NO 140, SEQ ID NO 143, SEQ ID NO 146, SEQ ID NO 149, SEQ ID NO 152, SEQ ID NO 155, and SEQ ID NO 158, or polypeptides having at least 80%, for example, at least 85%, for example, at least 90%, for example, at least 95%, or 100% sequence identity with them. The article is preferably a fabric, such as textiles, such as cotton and / or polyester.

[0536] Some aspects of the present invention relate to the use of DNase-active polypeptides for preventing, reducing, or removing biofilms from articles, wherein the DNase-active polypeptide comprises: polypeptides shown in SEQ ID NO 161, SEQ ID NO 164, SEQ ID NO 167, SEQ ID NO 170, SEQ ID NO 173, SEQ ID NO 176, SEQ ID NO 179, SEQ ID NO 182, SEQ ID NO 185, SEQ ID NO 188, SEQ ID NO 191, SEQ ID NO 194, and SEQ ID NO 197, or polypeptides having at least 80%, for example, at least 85%, for example, at least 90%, for example, at least 95%, or 100% sequence identity with them. The article is preferably a fabric, such as textiles, such as cotton and / or polyester.

[0537] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 11 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0538] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 12 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0539] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 13 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0540] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 14 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0541] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 15 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0542] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 16 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0543] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 17 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0544] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 18 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0545] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 19 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0546] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 20 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0547] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 21 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0548] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 22 or having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0549] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 23 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0550] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 53 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0551] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 56 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0552] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 59 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0553] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 62 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0554] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 65 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0555] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 68 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0556] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 71 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0557] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 74 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0558] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 77 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0559] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 80 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0560] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 83 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0561] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 86 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0562] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 89 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0563] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 92 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0564] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 95 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0565] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 98 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0566] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 101 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0567] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 104 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0568] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 107 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0569] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 110 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0570] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 113 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0571] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 116 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0572] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 119 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0573] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 122 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0574] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 125 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0575] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 128 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0576] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 131 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0577] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO134 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0578] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 137 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0579] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 140 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0580] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 143 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0581] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 146 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0582] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 149 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0583] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 152 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0584] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 155 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0585] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 158 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0586] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 161 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0587] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 164 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0588] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 167 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0589] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 170 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0590] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 173 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0591] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 176 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0592] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 179 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0593] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 182 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0594] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 185 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0595] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 188 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0596] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 191 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0597] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 194 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0598] Some aspects of the present invention relate to the use of polypeptides having DNase activity and comprising the amino acid sequence shown in SEQ ID NO 197 or a polypeptide having at least 80% sequence identity therewith for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0599] A particularly preferred aspect of the invention relates to DNases derived from the genus Bacillus, such as Bacillus species, preferably Bacillus species. 62451 DNases or DNases selected from the group consisting of closely related DNases, such as those with SEQ ID NO 8 (Bacillus species- 62451 The polypeptides with amino acid sequences shown in the diagram have at least 80% sequence identity with *Bacillus foodae*, *Bacillus* species-18318, and *Bacillus virologica* DNase. Preferred aspects of the invention relate to DNases obtained from *Bacillus* species (e.g., *Bacillus* species-18318). 62451 The use of a DNAseizure containing a polypeptide having at least 60%, such as at least 65%, at least 70%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 8 for the purpose of reducing odor from clothing and / or textiles, for anti-redeposition, and for maintaining or improving the whiteness of textiles. Preferred aspects of the invention relate to the use of a DNase derived from Bacillus (e.g., Bacillus foodae) comprising a polypeptide having at least 60%, for example, at least 65%, at least 70%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 21, for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles. Preferred aspects of the invention relate to the use of a DNase derived from the genus Bacillus (e.g., Bacillus species - 18318The use of a DNAseizure containing a polypeptide having at least 60%, such as at least 65%, at least 70%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 22 for the purpose of reducing odor from clothing and / or textiles, for anti-redeposition, and for maintaining or improving the whiteness of textiles. Preferred aspects of the invention relate to the use of a DNase derived from Bacillus (e.g., Bacillus cereus) containing a polypeptide having at least 60%, for example, at least 65%, at least 70%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with SEQ ID NO 23, for reducing odors from clothing and / or textiles, for preventing redeposition, and for maintaining or improving the whiteness of textiles.

[0600] As described above, the DNase peptides of the present invention possess exceptional deep cleaning capabilities; for example, the DNases of the present invention are particularly effective in disrupting or removing biofilms or components of biofilms (e.g., polysaccharides, proteins, DNA, dirt, or other components present in biofilms). Therefore, the DNase peptides of the present invention are particularly effective in preventing, reducing, or removing biofilms from articles such as textiles and hard surfaces.

[0601] The polypeptide with DNase activity is preferably obtained from Bacillus or Bacillus-like organisms. This invention relates to polypeptides having at least 60% sequence identity with any one of the mature polypeptides in SEQ ID NO: 2, 4, or 6, these polypeptides having DNase activity and wherein these polypeptides are used to prevent, reduce, or remove biofilms from articles. The invention further relates to polypeptides having at least 60%, for example 70%, for example 80%, or at least 90% sequence identity with any one of the mature polypeptides in SEQ ID NO: 2, 4, or 6, these polypeptides having DNase activity and wherein these polypeptides are used to prevent, reduce, or remove biofilms from articles. The present invention further relates to a polypeptide having at least 60%, for example 70%, for example 80%, or at least 90% sequence identity with any one of the polypeptides in SEQ ID NO 11, SEQ ID NO 12, SEQ ID NO 13, SEQ ID NO 14, SEQ ID NO 15, SEQ ID NO 16, SEQ ID NO 17, SEQ ID NO 18, SEQ ID NO 19, SEQ ID NO 20, SEQ ID NO 21, SEQ ID NO 22, or SEQ ID NO 23, having DNase activity and wherein such polypeptides can be used to prevent, reduce, or remove biofilms from an article.

[0602] In the embodiments, the present invention relates to polypeptides having at least 60% sequence identity with the polypeptide of SEQ ID NO: 8, these polypeptides having DNase activity and wherein the polypeptide is used to prevent, reduce or remove biofilms from an article.

[0603] In the embodiments, the present invention relates to polypeptides having at least 60% sequence identity with the polypeptide of SEQ ID NO: 9, these polypeptides having DNase activity and wherein the polypeptide is used to prevent, reduce or remove biofilms from an article.

[0604] In the embodiments, the present invention relates to polypeptides having at least 60% sequence identity with the polypeptide of SEQ ID NO: 10, these polypeptides having DNase activity and wherein the polypeptide is used to prevent, reduce or remove biofilms from an article.

[0605] In the embodiments, the present invention relates to polypeptides having at least 60% sequence identity with the polypeptide of SEQ ID NO: 11, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0606] In the embodiments, the present invention relates to polypeptides having at least 60% sequence identity with the polypeptide of SEQ ID NO: 12, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0607] In the embodiments, the present invention relates to polypeptides having at least 60% sequence identity with the polypeptide of SEQ ID NO: 13, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0608] In the embodiments, the present invention relates to polypeptides having at least 60% sequence identity with the polypeptide of SEQ ID NO: 14, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0609] In the embodiments, the present invention relates to polypeptides having at least 60% sequence identity with the polypeptide of SEQ ID NO: 15, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0610] In the embodiments, the present invention relates to polypeptides having at least 60% sequence identity with the polypeptide of SEQ ID NO: 16, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0611] In the embodiments, the present invention relates to polypeptides having at least 60% sequence identity with the polypeptide of SEQ ID NO: 17, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0612] In the embodiments, the present invention relates to polypeptides having at least 60% sequence identity with the polypeptide of SEQ ID NO: 18, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0613] In the embodiments, the present invention relates to polypeptides having at least 60% sequence identity with the polypeptide of SEQ ID NO: 19, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0614] In the embodiments, the present invention relates to polypeptides having at least 60% sequence identity with the polypeptide of SEQ ID NO: 20, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0615] In the embodiments, the present invention relates to polypeptides having at least 60% sequence identity with the polypeptide of SEQ ID NO: 21, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0616] In the embodiments, the present invention relates to polypeptides having at least 60% sequence identity with the polypeptide of SEQ ID NO: 22, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0617] In the embodiments, the present invention relates to polypeptides having at least 60% sequence identity with the polypeptide of SEQ ID NO: 23, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0618] In the embodiments, the present invention relates to polypeptides having at least 70% sequence identity with the polypeptide of SEQ ID NO: 8, the polypeptides having DNase activity and wherein the polypeptide is used to prevent, reduce or remove biofilms from an article.

[0619] In the embodiments, the present invention relates to polypeptides having at least 70% sequence identity with the polypeptide of SEQ ID NO: 9, these polypeptides having DNase activity and wherein the polypeptide is used to prevent, reduce or remove biofilms from an article.

[0620] In the embodiments, the present invention relates to polypeptides having at least 70% sequence identity with the polypeptide of SEQ ID NO: 10, the polypeptides having DNase activity and wherein the polypeptide is used to prevent, reduce or remove biofilms from an article.

[0621] In the embodiments, the present invention relates to polypeptides having at least 70% sequence identity with the polypeptide of SEQ ID NO: 11, having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0622] In the embodiments, the present invention relates to polypeptides having at least 70% sequence identity with the polypeptide of SEQ ID NO: 12, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0623] In the embodiments, the present invention relates to polypeptides having at least 70% sequence identity with the polypeptide of SEQ ID NO: 13, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0624] In the embodiments, the present invention relates to polypeptides having at least 70% sequence identity with the polypeptide of SEQ ID NO: 14, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0625] In the embodiments, the present invention relates to polypeptides having at least 70% sequence identity with the polypeptide of SEQ ID NO: 15, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0626] In the embodiments, the present invention relates to polypeptides having at least 70% sequence identity with the polypeptide of SEQ ID NO: 16, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0627] In the embodiments, the present invention relates to polypeptides having at least 70% sequence identity with the polypeptide of SEQ ID NO: 17, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0628] In the embodiments, the present invention relates to polypeptides having at least 70% sequence identity with the polypeptide of SEQ ID NO: 18, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0629] In the embodiments, the present invention relates to polypeptides having at least 70% sequence identity with the polypeptide of SEQ ID NO: 19, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0630] In the embodiments, the present invention relates to polypeptides having at least 70% sequence identity with the polypeptide of SEQ ID NO: 20, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0631] In the embodiments, the present invention relates to polypeptides having at least 70% sequence identity with the polypeptide of SEQ ID NO: 21, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0632] In the embodiments, the present invention relates to polypeptides having at least 70% sequence identity with the polypeptide of SEQ ID NO: 22, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0633] In the embodiments, the present invention relates to polypeptides having at least 70% sequence identity with the polypeptide of SEQ ID NO: 23, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0634] In the embodiments, the present invention relates to polypeptides having at least 80% sequence identity with the polypeptide of SEQ ID NO: 8, these polypeptides having DNase activity and wherein the polypeptide is used to prevent, reduce or remove biofilms from an article.

[0635] In the embodiments, the present invention relates to polypeptides having at least 80% sequence identity with the polypeptide of SEQ ID NO: 9, the polypeptides having DNase activity and wherein the polypeptide is used to prevent, reduce or remove biofilms from an article.

[0636] In the embodiments, the present invention relates to polypeptides having at least 80% sequence identity with the polypeptide of SEQ ID NO: 10, the polypeptides having DNase activity and wherein the polypeptide is used to prevent, reduce or remove biofilms from an article.

[0637] In the embodiments, the present invention relates to polypeptides having at least 80% sequence identity with the polypeptide of SEQ ID NO: 11, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0638] In the embodiments, the present invention relates to polypeptides having at least 80% sequence identity with the polypeptide of SEQ ID NO: 12, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0639] In the embodiments, the present invention relates to polypeptides having at least 80% sequence identity with the polypeptide of SEQ ID NO: 13, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0640] In the embodiments, the present invention relates to polypeptides having at least 80% sequence identity with the polypeptide of SEQ ID NO: 14, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0641] In the embodiments, the present invention relates to polypeptides having at least 80% sequence identity with the polypeptide of SEQ ID NO: 15, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0642] In the embodiments, the present invention relates to polypeptides having at least 80% sequence identity with the polypeptide of SEQ ID NO: 16, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0643] In the embodiments, the present invention relates to polypeptides having at least 80% sequence identity with the polypeptide of SEQ ID NO: 17, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0644] In the embodiments, the present invention relates to polypeptides having at least 80% sequence identity with the polypeptide of SEQ ID NO: 18, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0645] In the embodiments, the present invention relates to polypeptides having at least 80% sequence identity with the polypeptide of SEQ ID NO: 19, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0646] In the embodiments, the present invention relates to polypeptides having at least 80% sequence identity with the polypeptide of SEQ ID NO: 20, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0647] In the embodiments, the present invention relates to polypeptides having at least 80% sequence identity with the polypeptide of SEQ ID NO: 21, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0648] In the embodiments, the present invention relates to polypeptides having at least 80% sequence identity with the polypeptide of SEQ ID NO: 22, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0649] In the embodiments, the present invention relates to polypeptides having at least 80% sequence identity with the polypeptide of SEQ ID NO: 23, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0650] In the embodiments, the present invention relates to polypeptides having at least 85% sequence identity with the polypeptide of SEQ ID NO: 8, these polypeptides having DNase activity and wherein the polypeptide is used to prevent, reduce or remove biofilms from an article.

[0651] In the embodiments, the present invention relates to polypeptides having at least 85% sequence identity with the polypeptide of SEQ ID NO: 9, these polypeptides having DNase activity and wherein the polypeptide is used to prevent, reduce or remove biofilms from an article.

[0652] In the embodiments, the present invention relates to polypeptides having at least 85% sequence identity with the polypeptide of SEQ ID NO: 10, these polypeptides having DNase activity and wherein the polypeptide is used to prevent, reduce or remove biofilms from an article.

[0653] In the embodiments, the present invention relates to polypeptides having at least 85% sequence identity with the polypeptide of SEQ ID NO: 11, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0654] In the embodiments, the present invention relates to polypeptides having at least 85% sequence identity with the polypeptide of SEQ ID NO: 12, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0655] In the embodiments, the present invention relates to polypeptides having at least 85% sequence identity with the polypeptide of SEQ ID NO: 13, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0656] In the embodiments, the present invention relates to polypeptides having at least 85% sequence identity with the polypeptide of SEQ ID NO: 14, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0657] In the embodiments, the present invention relates to polypeptides having at least 85% sequence identity with the polypeptide of SEQ ID NO: 15, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0658] In the embodiments, the present invention relates to polypeptides having at least 85% sequence identity with the polypeptide of SEQ ID NO: 16, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0659] In the embodiments, the present invention relates to polypeptides having at least 85% sequence identity with the polypeptide of SEQ ID NO: 17, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0660] In the embodiments, the present invention relates to polypeptides having at least 85% sequence identity with the polypeptide of SEQ ID NO: 18, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0661] In the embodiments, the present invention relates to polypeptides having at least 85% sequence identity with the polypeptide of SEQ ID NO: 19, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0662] In the embodiments, the present invention relates to polypeptides having at least 85% sequence identity with the polypeptide of SEQ ID NO: 20, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0663] In the embodiments, the present invention relates to polypeptides having at least 85% sequence identity with the polypeptide of SEQ ID NO: 21, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0664] In the embodiments, the present invention relates to polypeptides having at least 85% sequence identity with the polypeptide of SEQ ID NO: 22, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0665] In the embodiments, the present invention relates to polypeptides having at least 85% sequence identity with the polypeptide of SEQ ID NO: 23, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0666] In the embodiments, the present invention relates to polypeptides having at least 90% sequence identity with the polypeptide of SEQ ID NO: 8, these polypeptides having DNase activity and wherein the polypeptide is used to prevent, reduce or remove biofilms from an article.

[0667] In the embodiments, the present invention relates to polypeptides having at least 90% sequence identity with the polypeptide of SEQ ID NO: 9, these polypeptides having DNase activity and wherein the polypeptide is used to prevent, reduce or remove biofilms from an article.

[0668] In the embodiments, the present invention relates to polypeptides having at least 90% sequence identity with the polypeptide of SEQ ID NO: 10, having DNase activity and wherein the polypeptide is used to prevent, reduce or remove biofilms from an article.

[0669] In the embodiments, the present invention relates to polypeptides having at least 90% sequence identity with the polypeptide of SEQ ID NO: 11, having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0670] In the embodiments, the present invention relates to polypeptides having at least 90% sequence identity with the polypeptide of SEQ ID NO: 12, having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0671] In the embodiments, the present invention relates to polypeptides having at least 90% sequence identity with the polypeptide of SEQ ID NO: 13, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0672] In the embodiments, the present invention relates to polypeptides having at least 90% sequence identity with the polypeptide of SEQ ID NO: 14, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0673] In the embodiments, the present invention relates to polypeptides having at least 90% sequence identity with the polypeptide of SEQ ID NO: 15, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0674] In the embodiments, the present invention relates to polypeptides having at least 90% sequence identity with the polypeptide of SEQ ID NO: 16, having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0675] In the embodiments, the present invention relates to polypeptides having at least 90% sequence identity with the polypeptide of SEQ ID NO: 17, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0676] In the embodiments, the present invention relates to polypeptides having at least 90% sequence identity with the polypeptide of SEQ ID NO: 18, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0677] In the embodiments, the present invention relates to polypeptides having at least 90% sequence identity with the polypeptide of SEQ ID NO: 19, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0678] In the embodiments, the present invention relates to polypeptides having at least 90% sequence identity with the polypeptide of SEQ ID NO: 20, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0679] In the embodiments, the present invention relates to polypeptides having at least 90% sequence identity with the polypeptide of SEQ ID NO: 21, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0680] In the embodiments, the present invention relates to polypeptides having at least 90% sequence identity with the polypeptide of SEQ ID NO: 22, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0681] In the embodiments, the present invention relates to polypeptides having at least 90% sequence identity with the polypeptide of SEQ ID NO: 23, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0682] In the embodiments, the present invention relates to polypeptides having at least 95% sequence identity with the polypeptide of SEQ ID NO: 8, these polypeptides having DNase activity and wherein the polypeptide is used to prevent, reduce or remove biofilms from an article.

[0683] In the embodiments, the present invention relates to polypeptides having at least 95% sequence identity with the polypeptide of SEQ ID NO: 9, these polypeptides having DNase activity and wherein the polypeptide is used to prevent, reduce or remove biofilms from an article.

[0684] In the embodiments, the present invention relates to polypeptides having at least 95% sequence identity with the polypeptide of SEQ ID NO: 10, these polypeptides having DNase activity and wherein the polypeptide is used to prevent, reduce or remove biofilms from an article.

[0685] In the embodiments, the present invention relates to polypeptides having at least 95% sequence identity with the polypeptide of SEQ ID NO: 11, having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0686] In the embodiments, the present invention relates to polypeptides having at least 95% sequence identity with the polypeptide of SEQ ID NO: 12, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0687] In the embodiments, the present invention relates to polypeptides having at least 95% sequence identity with the polypeptide of SEQ ID NO: 13, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0688] In the embodiments, the present invention relates to polypeptides having at least 95% sequence identity with the polypeptide of SEQ ID NO: 14, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0689] In the embodiments, the present invention relates to polypeptides having at least 95% sequence identity with the polypeptide of SEQ ID NO: 15, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0690] In the embodiments, the present invention relates to polypeptides having at least 95% sequence identity with the polypeptide of SEQ ID NO: 16, having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0691] In the embodiments, the present invention relates to polypeptides having at least 95% sequence identity with the polypeptide of SEQ ID NO: 17, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0692] In the embodiments, the present invention relates to polypeptides having at least 95% sequence identity with the polypeptide of SEQ ID NO: 18, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0693] In the embodiments, the present invention relates to polypeptides having at least 95% sequence identity with the polypeptide of SEQ ID NO: 19, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0694] In the embodiments, the present invention relates to polypeptides having at least 95% sequence identity with the polypeptide of SEQ ID NO: 20, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from an article.

[0695] In the embodiments, the present invention relates to polypeptides having at least 95% sequence identity with the polypeptide of SEQ ID NO: 21, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0696] In the embodiments, the present invention relates to polypeptides having at least 95% sequence identity with the polypeptide of SEQ ID NO: 22, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0697] In the embodiments, the present invention relates to polypeptides having at least 95% sequence identity with the polypeptide of SEQ ID NO: 23, the polypeptides having DNase activity and wherein the polypeptide can be used to prevent, reduce or remove biofilms from articles.

[0698] The preferred polypeptide of this invention is derived from Bacillus species- 62451 The claimed DNase (the mature polypeptide of SEQ ID NO 8 or SEQ ID NO 2), and is a closely related polypeptide having at least 80% sequence identity with the mature polypeptide having SEQ ID NO 8. Also claimed is Bacillus foodae SEQ ID NO 21, a species of the Bacillus genus. 18318 SEQ ID NO 22 and Bacillus spp. from the Institute of Pathogenic Biology SEQ ID NO 23, and a DNAseed that has at least 80% sequence identity with them.

[0699] The preferred polypeptide of the present invention is a DNase from Bacillus Horikoshii (the mature polypeptide of SEQ ID NO 9 or SEQ ID NO 4), and is a closely related polypeptide having at least 80% sequence identity with the mature polypeptide having SEQ ID NO 9. Homologous polypeptides also claimed are from Bacillus species— 62520 SEQ ID NO 11, Bacillus species - 62520 SEQ ID NO 12, Bacillus Horikoshi SEQ ID NO 13, Bacillus Horikoshi SEQ ID NO 14, Species of the genus Bacillus - 16840 SEQ ID NO 15, Bacillus species - 16840 SEQ ID NO 16, Bacillus species - 62668 SEQ ID NO 17, Bacillus species - 13395 SEQ ID NO 18, Bacillus honek-B, SEQ ID NO 19, Bacillus species- 11238 SEQ ID NO 20 and a DNA enzyme having at least 80% sequence identity with it.

[0700] The preferred polypeptide of this invention is derived from a species of the genus *Bacillus*. 18057The DNA enzyme (the mature polypeptide of SEQ ID NO 10 or SEQ ID NO 6), and is a closely related polypeptide with at least 80% sequence identity to the mature polypeptide having SEQ ID NO 10.

[0701] The deep cleaning effect of peptides with DNase activity, peptides having SEQ ID NO 8, 9 and 10 and homologous peptides having at least 80% identity with SEQ ID NO 8, 9 and 10 is shown in Example 2.

[0702] As stated above, the term "deep cleaning" refers to the disruption or removal of biofilms or components of biofilms, such as polysaccharides, proteins, DNA, dirt, or other components present in biofilms.

[0703] The polypeptide of the present invention preferably comprises or consists of the following: an amino acid sequence of any one of the amino acid sequences shown in SEQ ID NO: 8, 9 or 10, or an allelic variant thereof; or a fragment thereof having DNase activity. In another aspect, the polypeptide comprises or consists of the following: a mature polypeptide having any one of the amino acid sequences shown in SEQ ID NO: 2, 4 or 6.The polypeptides of the present invention preferably comprise or consist of the following items: SEQ ID NO 11, SEQ ID NO 12, SEQ ID NO 13, SEQ ID NO 14, SEQ ID NO 15, SEQ ID NO 16, SEQ ID NO 17, SEQ ID NO 18, SEQ ID NO 19, SEQ ID NO 20, SEQ ID NO 21, SEQ ID NO 22, SEQ ID NO 23, SEQ ID NO 53, SEQ ID NO 56, SEQ ID NO 59, SEQ ID NO 62, SEQ ID NO 65, SEQ ID NO 68, SEQ ID NO 71, SEQ ID NO 74, SEQ ID NO 77, SEQ ID NO 80, SEQ ID NO 83, SEQ ID NO 86, SEQ ID NO 89, SEQ ID NO 92, SEQ ID NO 95, SEQ ID NO 98, SEQ ID NO 101, SEQ ID NO 104, SEQ ID NO 107, SEQ ID NO 110, SEQ ID NO 104, SEQ ID NO 107, SEQ ID NO 110, SEQ ID NO 104, SEQ ID NO 105, SEQ ID NO 110, SEQ ID NO 106, SEQ ID NO 107, SEQ ID NO 110, SEQ ID NO 106, SEQ ID NO 107, SEQ ID NO 108, SEQ ID NO 109 ...9, SEQ ID NO 109, SEQ NO 113, SEQ ID NO 116, SEQ ID NO 119, SEQ ID NO 122, SEQ ID NO 125, SEQ ID NO 128, SEQ ID NO 131, SEQ ID NO 134, SEQ ID NO 137, SEQ ID NO 140, SEQ ID NO 143, SEQ ID NO 146, SEQ ID NO 149, SEQ ID NO 152, SEQ ID NO 155, SEQ ID NO 158, SEQ ID NO 161, SEQ ID NO 164, SEQ ID NO 167, SEQ ID NO 170, SEQ ID NO 173, SEQ ID NO 176, SEQ ID NO 179, SEQ ID NO 182, SEQ ID NO 185, SEQ ID NO 188, SEQ ID NO 191, SEQ ID NO 194 or SEQ ID NO The amino acid sequence of any of the amino acid sequences shown in 197, or its allele variants; or a fragment thereof having DNase activity.In another aspect, the polypeptide comprises or consists of any of the following: any one of SEQ ID NO 11, SEQ ID NO 12, SEQ ID NO 13, SEQ ID NO 14, SEQ ID NO 15, SEQ ID NO 16, SEQ ID NO 17, SEQ ID NO 18, SEQ ID NO 19, SEQ ID NO 20, SEQ ID NO 21, SEQ ID NO 22, SEQ ID NO 23, SEQ ID NO 53, SEQ ID NO 56, SEQ ID NO 59, SEQ ID NO 62, SEQ ID NO 65, SEQ ID NO 68, SEQ ID NO 71, SEQ ID NO 74, SEQ ID NO 77, SEQ ID NO 80, SEQ ID NO 83, SEQ ID NO 86, SEQ ID NO 89, SEQ ID NO 92, SEQ ID NO 95, SEQ ID NO 98, SEQ ID NO 101, SEQ ID NO 104, SEQ ID NO 107, SEQ ID NO 110. SEQ ID NO 113, SEQ ID NO 116, SEQ ID NO 119, SEQ ID NO 122, SEQ ID NO 125, SEQ ID NO 128, SEQ ID NO 131, SEQ ID NO 134, SEQ ID NO 137, SEQ ID NO 140, SEQ ID NO 143, SEQ ID NO 146, SEQ ID NO 149. SEQ ID NO 152, SEQ ID NO 155, SEQ ID NO 158, SEQ ID NO 161, SEQ ID NO 164, SEQ ID NO 167, SEQ ID NO 170, SEQ ID NO 173, SEQ ID NO 176, SEQ ID NO 179, SEQ ID NO 182, SEQ ID NO 185, SEQ ID NO 188, SEQ ID NO 191, SEQ ID NO 194 or SEQ ID NO The mature polypeptide of the polypeptide with the amino acid sequence shown in 197.

[0704] In one embodiment, the polypeptide has been isolated.

[0705] In another embodiment, the present invention relates to variants of mature polypeptides of SEQ ID NO: 2, 4, or 6, or having SEQ ID NO 11, SEQ ID NO 12, SEQ ID NO 13, SEQ ID NO 14, SEQ ID NO 15, SEQ ID NO 16, SEQ ID NO 17, SEQ ID NO 18, SEQ ID NO 19, SEQ ID NO 20, SEQ ID NO 21, SEQ ID NO 22, SEQ ID NO 23, SEQ ID NO 53, SEQ ID NO 56, SEQ ID NO 59, SEQ ID NO 62, SEQ ID NO 65, SEQ ID NO 68, SEQ ID NO 71, SEQ ID NO 74, SEQ ID NO 77, SEQ ID NO 80, SEQ ID NO 83, SEQ ID NO 86, SEQ ID NO 89, SEQ ID NO 92, SEQ ID NO 95, SEQ ID NO 98, SEQ ID NO 101, SEQ ID NO 104, SEQ ID NO 107, SEQ ID NO 108, SEQ ID NO 109, SEQ ID NO 10 ...9, SEQ ID NO 100, SEQ ID NO 109, SEQ ID NO 100, SEQ ID NO 100, SEQ ID NO 109, SEQ ID NO 100, SEQ ID NO 100, SEQ ID NO 100, SEQ ID NO 100, SEQ ID NO 100, SEQ ID NO 100, SEQ ID NO 100, SEQ ID NO 100, SEQ ID NO 100, SEQ ID ID NO 110, SEQ ID NO 113, SEQ ID NO 116, SEQ ID NO 119, SEQ ID NO 122, SEQ ID NO 125, SEQ ID NO 128, SEQ ID NO 131, SEQ ID NO 134, SEQ ID NO 137, SEQ ID NO 140, SEQ ID NO 143, SEQ ID NO 146, SEQ ID NO 149, SEQ ID NO 152, SEQ ID NO 155, SEQ ID NO 158, SEQ ID NO 161, SEQ ID NO 164, SEQ ID NO 167, SEQ ID NO 170, SEQ ID NO 173, SEQ ID NO 176, SEQ ID NO 179, SEQ ID NO 182, SEQ ID NO 185, SEQ ID NO 188, SEQ ID NO 191, SEQ ID NO 194 or SEQ ID NO Variants of any homologous polypeptide of the amino acid sequence shown in 197, which include substitutions, deletions, and / or insertions at one or more (e.g., several) positions.In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the mature polypeptide of SEQ ID NO: 2, 4, or 6 is up to 10, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0706] In another embodiment, the present invention relates to variants of a mature polypeptide comprising substitutions, deletions, and / or insertions of SEQ ID NO: 8 at one or more (e.g., several) locations. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 8 is up to 10, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0707] In another embodiment, the present invention relates to variants of a mature polypeptide comprising substitutions, deletions, and / or insertions of SEQ ID NO: 9 at one or more (e.g., several) locations. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 9 is up to 10, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0708] In another embodiment, the present invention relates to variants of the mature polypeptide of SEQ ID NO: 10 comprising substitutions, deletions, and / or insertions at one or more (e.g., several) locations. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 10 is up to 10, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0709] In another embodiment, the present invention relates to variants of the mature polypeptide of SEQ ID NO: 11 comprising substitutions, deletions, and / or insertions at one or more (e.g., several) sites. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 11 is up to 10, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0710] In another embodiment, the present invention relates to variants of a mature polypeptide comprising substitutions, deletions, and / or insertions of SEQ ID NO: 12 at one or more (e.g., several) locations. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions in the polypeptide incorporating SEQ ID NO: 12 is up to 10, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0711] In another embodiment, the present invention relates to variants of a mature polypeptide comprising substitutions, deletions, and / or insertions of SEQ ID NO: 13 at one or more (e.g., several) locations. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions in the polypeptide incorporating SEQ ID NO: 13 is up to 10, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0712] In another embodiment, the present invention relates to variants of a mature polypeptide comprising substitutions, deletions, and / or insertions of SEQ ID NO: 14 at one or more (e.g., several) locations. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions in the polypeptide incorporating SEQ ID NO: 14 is up to 10, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0713] In another embodiment, the present invention relates to variants of a mature polypeptide comprising substitutions, deletions, and / or insertions of SEQ ID NO: 15 at one or more (e.g., several) locations. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions in the polypeptide incorporating SEQ ID NO: 15 is up to 10, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0714] In another embodiment, the present invention relates to variants of a mature polypeptide comprising substitutions, deletions, and / or insertions of SEQ ID NO: 16 at one or more (e.g., several) locations. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions in the polypeptide incorporating SEQ ID NO: 16 is up to 10, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0715] In another embodiment, the present invention relates to a variant of a mature polypeptide comprising substitutions, deletions, and / or insertions of SEQ ID NO: 17 at one or more (e.g., several) locations. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions in the polypeptide incorporating SEQ ID NO: 17 is up to 10, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0716] In another embodiment, the present invention relates to variants of a mature polypeptide comprising substitutions, deletions, and / or insertions of SEQ ID NO: 18 at one or more (e.g., several) locations. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions in the polypeptide incorporating SEQ ID NO: 18 is up to 10, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0717] In another embodiment, the present invention relates to variants of a mature polypeptide comprising substitutions, deletions, and / or insertions of SEQ ID NO: 19 at one or more (e.g., several) locations. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions in the polypeptide incorporating SEQ ID NO: 19 is up to 10, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0718] In another embodiment, the present invention relates to variants of a mature polypeptide comprising substitutions, deletions, and / or insertions of SEQ ID NO: 20 at one or more (e.g., several) sites. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 20 is up to 10, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0719] In another embodiment, the present invention relates to variants of a mature polypeptide comprising substitutions, deletions, and / or insertions of SEQ ID NO: 21 at one or more (e.g., several) locations. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions in the polypeptide incorporating SEQ ID NO: 21 is up to 10, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0720] In another embodiment, the present invention relates to variants of a mature polypeptide comprising substitutions, deletions, and / or insertions of SEQ ID NO: 22 at one or more (e.g., several) locations. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions in the polypeptide incorporating SEQ ID NO: 22 is up to 10, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0721] In another embodiment, the present invention relates to a variant of a mature polypeptide comprising substitutions, deletions, and / or insertions of SEQ ID NO: 23 at one or more (e.g., several) locations. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions in the polypeptide incorporating SEQ ID NO: 23 is up to 10, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0722] These amino acid alterations can be minor, i.e., conserved amino acid substitutions or insertions that do not significantly affect protein folding and / or activity; small deletions, typically 1–30 amino acids; small N-terminal or C-terminal extensions, such as methionine residues at the N-terminus; small linker peptides of up to 20–25 residues; or small extensions that facilitate purification by altering net charge or another function, such as polyhistidine fragments, antigenic epitopes, or binding domains.

[0723] Examples of conserved substitutions are found in the following groups: basic amino acids (arginine, lysine, and histidine), acidic amino acids (glutamic acid and aspartic acid), polar amino acids (glutamine and asparagine), hydrophobic amino acids (leucine, isoleucine, and valine), aromatic amino acids (phenylalanine, tryptophan, and tyrosine), and small amino acids (glycine, alanine, serine, threonine, and methionine). Amino acid substitutions that generally do not alter specific reactivity are known in the art and, for example, by H. Neurath and RL Hill, 1979. The Proteins [Proteins], Academic Press, New York. Common substitutions are Ala / Ser, Val / Ile, Asp / Glu, Thr / Ser, Ala / Gly, Ala / Thr, Ser / Asn, Ala / Val, Ser / Gly, Tyr / Phe, Ala / Pro, Lys / Arg, Asp / Asn, Leu / Ile, Leu / Val, Ala / Glu, and Asp / Gly.

[0724] Alternatively, these amino acid changes have the property of altering the physicochemical properties of the peptide. For example, amino acid changes can improve the peptide's thermal stability, alter substrate specificity, change the optimal pH, etc.

[0725] It can be performed according to methods known in the art, such as site-directed mutagenesis or alanine scanning mutagenesis (Cunningham and Wells, 1989, Science (244: 1081-1085) to identify essential amino acids in polypeptides. In the latter technique, a single alanine mutation is introduced at each residue in the molecule, and the DNase activity of the resulting mutant molecule is tested to identify amino acid residues essential for the molecule's activity. See also Hilton et al., 1996. J. Biol.Chem [Journal of Biochemistry] 271: 4699-4708. The active site of the enzyme or other biological interactions can also be determined by physical analysis of the structure, such as by techniques like nuclear magnetic resonance, crystallography, electron diffraction, or photoaffinity labeling, based on mutations in the amino acids at the hypothetical contact site. See, for example, de Vos et al., 1992. Science [Science] 255: 306-312; Smith et al., 1992, J. Mol. Biol. [Journal of Molecular Biology] 224:899-904; Wlodaver et al., 1992. FEBS Lett.[Circular of the Federation of European Biochemical Societies] 309: 59-64. Use of a polypeptide having DNase activity [Uses of polypeptides with DNase activity] 3: 568-576; Svetina et al., 2000, J. Biotechnol [Journal of Biotechnology] 76: 245-251; Rasmussen-Wilson et al., 1997, Appl.Environ.Microbiol. [Applied and Environmental Microbiology] 63: 3488-3493; Ward et al., 1995. Biotechnology [Biotechnology] 13: 498-503; and Contreras et al., 1991, Biotechnology [Biotechnology] 9: 378-381; Eaton et al., 1986, Biochemistry [Biochemistry] 25: 505-512; Collins-Racie et al., 1995, Biotechnology [Biotechnology] 13: 982-987; Carter et al., 1989, Proteins: Structure, Function, and Genetics [Proteins: Structure, Function, and Genetics] 6: 240-248; and Stevens, 2003. Drug Discovery World [Drug Discovery World] 4:35-48.

[0726] Polynucleotides

[0727] The present invention also relates to polynucleotides encoding polypeptides as described herein. In one embodiment, the polynucleotide encoding the polypeptide of the present invention has been isolated. In another embodiment, the polynucleotide encoding the polypeptide of the present invention comprises, or is composed of, the polynucleotide sequence illustrated in SEQ ID NO: 1, 3 or 5.

[0728] In another embodiment, the present invention relates to an isolated polypeptide with DNase activity, the isolated polypeptide being encoded by a polynucleotide hybridized under low stringency conditions to (i) the mature polypeptide coding sequence of SEQ ID NO: 1, 3 or 5, or (ii) the full-length complement of (i) (Sambrook et al., 1989, Molecular Cloning, A Laboratory Manual, 2nd Edition, Cold Spring Harbor, New York). In one embodiment, the polypeptide has been isolated.

[0729] In another embodiment, the present invention relates to an isolated polypeptide having DNase activity, the isolated polypeptide being encoded by a polynucleotide hybridized under low-to-medium stringent conditions to (i) the coding sequence of a mature polypeptide of SEQ ID NO: 1, 3 or 5, or (ii) the full-length complement of (i). In one embodiment, the polypeptide has been isolated.

[0730] In another embodiment, the present invention relates to an isolated polypeptide having DNase activity, the isolated polypeptide being encoded by a polynucleotide hybridized under stringent conditions to (i) the coding sequence of a mature polypeptide of SEQ ID NO: 1, 3 or 5, or (ii) the full-length complement of (i). In one embodiment, the polypeptide has been isolated.

[0731] In another embodiment, the present invention relates to an isolated polypeptide with DNase activity, the isolated polypeptide being encoded by a polynucleotide hybridized under medium- to high stringency conditions to (i) the coding sequence of a mature polypeptide of SEQ ID NO: 1, 3 or 5, or (ii) the full-length complement of (i). In one embodiment, the polypeptide has been isolated.

[0732] In another embodiment, the present invention relates to an isolated polypeptide having DNase activity, the isolated polypeptide being encoded by a polynucleotide hybridized under highly stringent conditions to (i) the coding sequence of a mature polypeptide of SEQ ID NO: 1, 3 or 5, or (ii) the full-length complement of (i). In one embodiment, the polypeptide has been isolated.

[0733] In another embodiment, the present invention relates to an isolated polypeptide with DNase activity, the isolated polypeptide being encoded by a polynucleotide hybridized under very stringent conditions to (i) the coding sequence of a mature polypeptide of SEQ ID NO: 1, 3 or 5, or (ii) the full-length complement of (i). In one embodiment, the polypeptide has been isolated.

[0734] Nucleic acid probes can be designed using polynucleotides or subsequences thereof of SEQ ID NO: 1, 3, or 5, together with polypeptides or fragments thereof of SEQ ID NO: 2, 4, or 6, or polypeptides or fragments thereof of SEQ ID NO: 8, 9, or 10, to identify and clone DNA encoding polypeptides with DNase activity from strains of different genera or species, according to methods well known in the art. Specifically, such probes can be used to hybridize with the genomic DNA of cells of interest following standard DNA blotting procedures to identify and isolate the corresponding genes therein. Such probes may be significantly shorter than the complete sequence, but should be at least 15, for example at least 25, at least 35, or at least 70 nucleotides in length. Preferably, the nucleic acid probe is at least 100 nucleotides in length, for example at least 200, at least 300, at least 400, at least 500, or at least 600 nucleotides in length. Both DNA and RNA probes can be used. Probes are typically labeled (e.g., with...) 32 P, 3 H, 35 (S, biotin, or avidin) to detect the corresponding gene. This invention covers such probes.

[0735] DNA from libraries of genomic DNA or cDNA (when the polypeptide contains introns) prepared from other strains of this type can be screened for DNA that hybridizes to the probes described above and encodes polypeptides with DNase activity. Genomic DNA or other DNA from other strains of this type can be separated by agarose or polyacrylamide gel electrophoresis or other separation techniques. DNA from the library or separated DNA can be transferred and immobilized on nitrocellulose or other suitable carrier materials. To identify clones or DNA that hybridize to SEQ ID NO:1, 3, or 5 or their subsequences, the carrier material is used for DNA blotting.

[0736] For the purposes of this invention, hybridization refers to the hybridization of a polynucleotide to a nucleic acid probe corresponding to the following labeled: (i) SEQ ID NO: 1, 3, or 5; (ii) the coding sequence of a mature polypeptide of SEQ ID NO: 1, 3, or 5; (iii) its full-length complement; or (iv) its subsequence; performed under very low, low stringency, low-to-medium stringency, medium stringency, medium-to-high stringency, high stringency, and very high stringency conditions. Molecules hybridizing with the nucleic acid probe under these conditions can be detected using, for example, X-ray film or any other detection method known in the art.

[0737] In another embodiment, the present invention relates to a polypeptide having DNase activity, encoded by a polynucleotide having at least 60%, for example at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with the mature polypeptide coding sequence of SEQ ID NO: 1, 3, or 5. In another embodiment, the polypeptide has been isolated.

[0738] In another embodiment, the present invention relates to a polypeptide having DNase activity, comprising SEQ ID NO25, SEQ ID NO27, SEQ ID NO29, SEQ ID NO31, SEQ ID NO33, SEQ ID NO35, SEQ ID NO37, SEQ ID NO39, SEQ ID NO41, SEQ ID NO43, SEQ ID NO45, SEQ ID NO47, SEQ ID NO49, SEQ ID NO51, SEQ ID NO54, SEQ ID NO57, SEQ ID NO60, SEQ ID NO63, SEQ ID NO66, SEQ ID NO69, SEQ ID NO72, SEQ ID NO75, SEQ ID NO78, SEQ ID NO81, SEQ ID NO84, SEQ ID NO87, SEQ ID NO90, SEQ ID NO93, SEQ ID NO96, SEQ ID NO99, SEQ ID NO102, SEQ ID NO105, SEQ ID NO108, SEQ ID NO109 ... ID NO 111, SEQ ID NO 114, SEQ ID NO 117, SEQ ID NO 120, SEQ ID NO 123, SEQ ID NO 126, SEQ ID NO 129, SEQ ID NO 132, SEQ ID NO 135, SEQ ID NO 138, SEQ ID NO 141, SEQ ID NO 144, SEQ ID NO 147, SEQ ID NO 150, SEQ ID NO 153, SEQ ID NO 156, SEQ ID NO 159, SEQ ID NO 162, SEQ ID NO 165, SEQ ID NO 168, SEQ ID NO 171, SEQ ID NO 174, SEQ ID NO 177, SEQ ID NO 180, SEQ ID NO 183, SEQ ID NO 186, SEQ ID NO 189、SEQ ID NO 192、SEQ ID NO The mature polypeptide coding sequence of 195 has a polynucleotide coding with at least 60%, for example, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence consistency.In another embodiment, the polypeptide has been isolated.

[0739] Techniques for isolating or cloning polynucleotides are known in the art and include isolation from genomic DNA, or combinations thereof. Cloning polynucleotides from genomic DNA can be achieved, for example, by using well-known polymerase chain reaction (PCR) or antibody screening of expression libraries to detect cloned DNA fragments with shared structural features. See, for example, Innis et al., 1990. PCR: A Guide to Methods and Application [PCR: Methods and Applications Guide], Academic Press, New York. Other nucleic acid amplification procedures can be used, such as ligase chain reaction (LCR), ligation-activated transcription (LAT), and polynucleotide-based amplification (NASBA).

[0740] Modification of the polynucleotide encoding the polypeptide of the present invention may be necessary for the synthesis of polypeptides substantially similar to the polypeptide. The term "substantially similar" to the polypeptide refers to a non-naturally occurring form of the polypeptide. These polypeptides may differ from polypeptides isolated from their natural sources due to some form of engineering, for example, in variations in specific activity, thermal stability, optimal pH, etc. Variants can be constructed as follows: based on a polynucleotide proposed as the coding sequence (e.g., a subsequence of) a mature polypeptide as SEQ ID NO: 1, 3, or 5, and / or by introducing nucleotide substitutions that do not result in a change in the amino acid sequence of the polypeptide but correspond to the codon usage intended for use in the host organism producing the enzyme, or by introducing nucleotide substitutions that can produce different amino acid sequences. For a general description of nucleotide substitutions, see, for example, Ford et al., 1991. Protein Expression and Purification [Protein Expression and Purification] 2: 95-107.

[0741] Nucleic acid constructs

[0742] The present invention also relates to nucleic acid constructs comprising a polynucleotide of the present invention efficiently linked to one or more control sequences, which, under conditions compatible with the control sequences, guide the expression of a coding sequence in a suitable host cell.

[0743] The polynucleotide can be manipulated in many ways to provide polypeptide expression. Depending on the expression vector, manipulating the polynucleotide prior to insertion into the vector may be desirable or necessary. Techniques for modifying polynucleotides using recombinant DNA methods are known in the art.

[0744] The control sequence may be a promoter, i.e., a polynucleotide recognized by the host cell for expressing a polynucleotide encoding the polypeptide of the present invention. The promoter contains a transcriptional control sequence that mediates the expression of the polypeptide. The promoter may be any polynucleotide exhibiting transcriptional activity in the host cell, including mutant, truncated, and heterozygous promoters, and may be derived from a gene encoding an extracellular or intracellular polypeptide that is homologous or heterologous to the host cell.

[0745] Examples of suitable promoters for directing the transcription of the nucleic acid constructs of the present invention in bacterial host cells are promoters obtained from the following gene: Bacillus amyloliquefaciens α-amylase gene ( amyQ ), Bacillus licheniformis α-amylase gene ( amyL) Bacillus licheniformis penicillinase gene ( penP ), Bacillus stearothermophilus maltose amylase gene ( amyM ), Bacillus subtilis fructan sucrase gene ( sacB Bacillus subtilis xylA and xylB Genes, Bacillus thuringiensis cryIIIA Genes (Agaisse and Lereclus, 1994, Molecular Microbiology [Molecular Microbiology] 13: 97-107), Escherichia coli lac operon, E. coli trc Promoters (Egon et al., 1988, Gene [Gene] 69: 301-315), Streptomyces agar hydrolase gene ( dagA ) and prokaryotic β-lactamase genes (Villa-Kamaroff et al., 1978, Proc. Natl. Acad. Sci. USA [Proceedings of the National Academy of Sciences of the United States of America] 75: 3727-3731) and tac Promoters (DeBoer et al., 1983, Proc. Natl. Acad. Sci. USA [Proceedings of the National Academy of Sciences of the United States of America] 80: 21-25). Other promoters are described in Gilbert et al., 1980. Scientific American In “Useful proteins from recombinant bacteria”, 242: 74-94; and Sambrook et al., 1989, see above. Examples of tandem promoters are disclosed in WO 99 / 43835.

[0746] In filamentous fungal host cells, an example of a suitable promoter for guiding the transcription of the nucleic acid constructs of this invention is a promoter derived from the gene of the enzyme Aspergillus nidulans (…). Aspergillus nidulans Acetamipridase, Aspergillus niger ( Aspergillus niger Neutral α-amylase, aspergillus acid-stable α-amylase, Aspergillus niger or Aspergillus foetida ( Aspergillus awamori ) glucosylamylase ( glaA Aspergillus oryzae ( ) Aspergillus oryzae TAKA amylase, Aspergillus oryzae alkaline protease, Aspergillus oryzae triose phosphate isomerase, Fusarium oxysporum ( Fusarium oxysporum Trypsin-like protease (WO 96 / 00787), Fusarium moniliforme ( Fusarium venenatum Amylase (WO 00 / 56900), *Daria porphyria* (WO 00 / 56900), *Quinn. porphyria* (WO 00 / 56900), *Rhizopus mellea* (WO 00 / 56900) Rhizomucor miehei Lipase, aspartic acid protease from *Rhizopus oryzae*, and *Trichoderma reesei* ( Trichoderma reesei β-glucosidase, *Trichoderma reesei* cellobiase I, *Trichoderma reesei* cellobiase II, *Trichoderma reesei* endoglucanase I, *Trichoderma reesei* endoglucanase II, *Trichoderma reesei* endoglucanase III, *Trichoderma reesei* endoglucanase V, *Trichoderma reesei* xylanase I, *Trichoderma reesei* xylanase II, *Trichoderma reesei* xylanase III, *Trichoderma reesei* β-xylosidase, and *Trichoderma reesei* translation elongation factor, and NA2-tpi promoter ( Modified promoters from Aspergillus neutral α-amylase genes, wherein the untranslated leader sequence has been replaced with an untranslated leader sequence from Aspergillus triose phosphate isomerase genes; non-limiting examples include modified promoters from Aspergillus niger neutral α-amylase genes, wherein the untranslated leader sequence has been replaced with an untranslated leader sequence from Aspergillus nidulans or Aspergillus oryzae triose phosphate isomerase genes, and their mutant, truncated, and heterozygous promoters. Other promoters are described in U.S. Patent No. 6,011,147.

[0747] In yeast hosts, useful promoters are obtained from the genes of the following enzymes: Saccharomyces cerevisiae (Saccharomyces cerevisiae). Saccharomyces cerevisiae Enolase (ENO-1), *Saccharomyces cerevisiae* galactokinase (GAL1), *Saccharomyces cerevisiae* alcohol dehydrogenase / glyceraldehyde-3-phosphate dehydrogenase (ADH1, ADH2 / GAP), *Saccharomyces cerevisiae* triose phosphate isomerase (TPI), *Saccharomyces cerevisiae* metallothionein (CUP1), and *Saccharomyces cerevisiae* 3-phosphate glycerate kinase. Romanos et al., 1992, Yeast [Yeast] 8: 423-488 describes other useful promoters for yeast host cells.

[0748] The control sequence can also be a transcription terminator recognized by the host cell to terminate transcription. This terminator is effectively linked to the 3' end of the polynucleotide encoding the polypeptide. Any terminator that is functional in the host cell can be used in this invention.

[0749] The preferred terminator for bacterial host cells is obtained from the genes of the following enzymes: Bacillus clausti ( Bacillus clausii Alkaline protease (aprH), Bacillus licheniformis α-amylase (amyL), and Escherichia coli ribosomal RNA (rrnB).

[0750] Preferred terminators for filamentous fungal host cells are derived from the genes of the following enzymes: Aspergillus nidulans acetamase, Aspergillus nidulans o-aminobenzoic acid synthase, Aspergillus niger glucosylamylase, Aspergillus niger α-glucosidase, Aspergillus oryzae TAKA amylase, Fusarium oxysporum trypsin-like protease, Trichoderma reesei β-glucosidase, Trichoderma reesei cellobiose hydrolase I, Trichoderma reesei cellobiose hydrolase II, Trichoderma reesei endoglucanase I, Trichoderma reesei endoglucanase II, Trichoderma reesei endoglucanase III, Trichoderma reesei endoglucanase V, Trichoderma reesei xylanase I, Trichoderma reesei xylanase II, Trichoderma reesei xylanase III, Trichoderma reesei β-xylosidase, and Trichoderma reesei translation elongation factor.

[0751] Preferred terminators for yeast host cells are derived from the genes of the following enzymes: *Saccharomyces cerevisiae* enolase, *Saccharomyces cerevisiae* cytochrome C (CYC1), and *Saccharomyces cerevisiae* glyceraldehyde-3-phosphate dehydrogenase. Other useful terminators for yeast host cells are described above in Romanos et al., 1992.

[0752] Control sequences can also be mRNA stabilizing regions downstream of the promoter and upstream of the gene's coding sequence, which increase the expression of the gene.

[0753] Examples of suitable mRNA stable regions were obtained from Bacillus thuringiensis. cryIIIA Gene (WO94 / 25612) and Bacillus subtilis SP82 gene (Hue et al., 1995, Journal of Bacteriology 177: 3465-3471).

[0754] This control sequence can also be a leader sequence, the untranslated region of mRNA important for translation in the host cell. This leader sequence is operatively linked to the 5' end of the polynucleotide encoding the polypeptide. Any leader sequence that is functional in the host cell can be used.

[0755] The preferred leader sequence for use in filamentous fungal host cells was obtained from the genes of Aspergillus oryzae TAKA amylase and Aspergillus nidulans triose phosphate isomerase.

[0756] Suitable leader sequences for yeast host cells are obtained from the genes of the following enzymes: Saccharomyces cerevisiae enolase (ENO-1), Saccharomyces cerevisiae 3-phosphoglycerate kinase, Saccharomyces cerevisiae α factor, and Saccharomyces cerevisiae alcohol dehydrogenase / glyceraldehyde-3-phosphate dehydrogenase (ADH2 / GAP).

[0757] The control sequence can also be a polyadenylation sequence, a sequence that is efficiently linked to the 3' end of the polynucleotide and, when transcribed, is recognized by the host cell as a signal to add polyadenylation residues to the transcribed mRNA. Any polyadenylation sequence that is functional in the host cell can be used.

[0758] Preferred polyadenylated sequences for use in filamentous fungal host cells were obtained from the genes of the following enzymes: Aspergillus nidulans o-aminobenzoic acid synthase, Aspergillus niger glucosidase, Aspergillus niger α-glucosidase, Aspergillus oryzae TAKA amylase, and Fusarium oxysporum trypsin-like protease.

[0759] The polyadenylation sequences useful for yeast host cells were proposed by Guo and Sherman, 1995. Mol. Molecular cell biology (Mol. Cellular Biol.) 15: 5983-5990 Description.

[0760] The control sequence can also be a signal peptide coding region encoding a signal peptide that is linked to the N-terminus of a polypeptide and guides the polypeptide into the secretory pathway of the cell. The 5' end of the coding sequence of a polynucleotide may inherently contain a signal peptide coding sequence naturally linked to a segment of the coding sequence of the polypeptide within the translation reading frame. Alternatively, the 5' end of the coding sequence may contain a signal peptide coding sequence that is exogenous to the coding sequence. In cases where the coding sequence does not naturally contain a signal peptide coding sequence, ...

Claims

1. A detergent composition comprising at least 0.002 ppm of a polypeptide with DNase activity and at least one detergent adjuvant ingredient, wherein the polypeptide with DNase activity is selected from the group consisting of: a) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

8. b) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

9. c) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

10. d) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

11. e) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

12. f) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

13. g) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

14. h) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

15. i) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

16. j) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

17. k) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

18. l) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

19. m) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

20. n) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

21. o) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

22. p) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

23. q) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

53. r) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

56. s) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

59. t) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

62. u) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

65. v) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

68. w) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

71. x) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

74. y) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

77. z) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

80. aa) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

83. (bb) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

86. (cc) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

89. (dd) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

92. (ee) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

95. (ff) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

98. A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

101. (hh) A polypeptide that has at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

104. ii) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

107. (jj) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO: 110, (kk) A polypeptide that has at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

113. ll) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

116. A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO: 119 (mm). (nn) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO: 122, (oo) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO: 125, (pp) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

128. (qq) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

131. (rr) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

134. ss) a polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO: 137, (tt) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO: 140, (uu) is a polypeptide that has at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

143. (vv) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

146. A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

149. (xx) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

152. (yy) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

155. (zz) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

158. aaa) is a polypeptide that has at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

161. (bbb) A polypeptide that has at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

164. A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

167. (ddd) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

170. eee) is a polypeptide that has at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

173. (fff) A polypeptide that has at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

176. (ggg) is a polypeptide that has at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

179. (hhh) A polypeptide that has at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

182. iii) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

185. (jjj) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO: 188, (kkk) is a polypeptide that has at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

191. (lll) A polypeptide having at least 80% sequence identity with the polypeptide shown in SEQ ID NO: 194, and The polypeptide (mmm) has at least 80% sequence identity with the polypeptide shown in SEQ ID NO:

197.

2. The detergent composition according to claim 1, wherein the polypeptide having DNase activity comprises the motif HXXP, wherein H is histidine, P is proline, and X is any amino acid.

3. The detergent composition of claim 1, wherein the polypeptide having DNase activity comprises: One or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO:204) or ASXNRSKG (SEQ ID NO:205); or One or both motifs of [V / I]PL[S / A]NAWK (SEQ ID NO: 206) or NPQL (SEQ ID NO: 207); or One or both of the motifs of P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209).

4. The detergent composition of claim 1, wherein the polypeptide having DNase activity has at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO:

122.

5. The detergent composition of claim 1, wherein the detergent adjuvant component comprises at least one surfactant or detergent builder.

6. The detergent composition of claim 1, wherein the composition comprises at least one anionic surfactant.

7. The detergent composition of claim 1, wherein the composition is formulated as a strip, a uniform tablet, a tablet having two or more layers, a bag having one or more chambers, a regular or compressed powder, granules, paste, gel, or a regular, compressed or concentrated liquid.

8. The detergent composition of claim 1, wherein the composition comprises: i. One or more polyols, preferably selected from glycerol, (mono, di, or tri) propylene glycol, ethylene glycol, polyethylene glycol, sugar alcohols, sorbitol, mannitol, erythritol, galactitol, inositol, xylitol, and adonitol. ii. Optionally, one or more enzymes, preferably selected from proteases, amylases, or lipases. iii. Optionally, one or more surfactants, preferably selected from anionic and nonionic surfactants, and iv. Optionally, one or more polymers.

9. The detergent composition of claim 1, wherein the composition is in the form of granules, the granules comprising... i. A core, comprising a polypeptide with DNase activity, and optionally, ii. Coating, which consists of one or more layers surrounding the core.

10. The detergent composition of claim 1 further comprises at least one enzyme selected from the following: protease, lipase, keratinase, amylase, glycosylase, cellulase, pectinase, mannanase, arabinase, galactanase, xylanase, and oxidase.

11. The detergent composition according to claim 1, wherein the composition is a laundry detergent composition.

12. Use of a polypeptide having DNase activity for preventing, reducing or removing biofilms from articles, wherein the polypeptide having DNase activity is as defined in any one of claims 1-4.

13. The use according to claim 12, wherein the article is a textile.

14. Use of a polypeptide having DNase activity for reducing odor from clothing and / or textiles, for preventing redeposition, or for maintaining or improving the whiteness of textiles, wherein the polypeptide having DNase activity is as defined in any one of claims 1-4.

15. A method for washing textiles, comprising: a) Exposing the textile to a detergent containing a polypeptide with DNase activity, wherein the polypeptide with DNase activity is defined as in any one of claims 1-4, or exposing it to a detergent composition defined as in any one of claims 1-11; b) Complete at least one washing cycle; and optionally c) Rinse the textile.

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