A liquid fermentation culture device for morel mushrooms that facilitates mycelial inoculation

CN122563692APending Publication Date: 2026-08-14JINING UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-05-13
Publication Date
2026-08-14

AI Technical Summary

Technical Problem

[0006]本发明的目的是提供一种便于菌丝接种的羊肚菌液体发酵培养设备,通过设计接种与通气一体化的密封通道结构,集成双重对向独立密封、接种工具在线消毒及自动化批量无菌通气功能,以解决现有技术中羊肚菌液体发酵培养存在的接种需开盖导致大面积污染风险高、接种工具易带入外源杂菌、通气与接种分开开孔增加污染位点、批量培养易发生单元间交叉污染,以及操作繁琐、自动化程度低的问题

Benefits of technology

本设备通过盖子实现培养底皿的基础密封,配合推筒开口边缘密封圈与环形槽内壁形成的动密封,构建基础无菌屏障;核心采用梯形板一与梯形板二组成的双重独立密封结构。接种全程无需打开盖子,仅通过推筒的上下移动即可完成操作,彻底避免了传统开盖接种带来的大面积污染风险;同时锥形橡胶密封筒可弹性紧密包裹接种试管外壁实现动态密封,其底部环形盛放槽内的消毒液可对试管外壁进行浸润擦拭消毒,从源头阻断杂菌随接种工具带入培养体系,全面保障菌丝接种过程的无菌安全性;

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Abstract

This invention relates to the field of mycelial fermentation culture, specifically to a liquid fermentation culture device for morel mushrooms that facilitates mycelial inoculation. The device includes a fermentation culture cabinet with a door rotatably connected to one side of the opening, and load-bearing partitions slidably connected sequentially inside the cabinet. This invention achieves a basic seal on the culture dish through a lid, and constructs a basic sterile barrier through a dynamic seal with a pusher. The core employs a double independent sealing structure composed of trapezoidal plate one and trapezoidal plate two. Inoculation does not require opening the lid; the operation is completed solely by the up-and-down movement of the pusher, avoiding the large-area contamination associated with traditional open-lid inoculation. Simultaneously, a conical rubber sealing cylinder wraps around the inoculation tube to achieve a dynamic seal, and the disinfectant at its bottom can be used to wipe the outer wall of the tube online, preventing the introduction of contaminating bacteria. During the culture stage, an electric pusher drives the vent pipe to connect to the pusher. Air is introduced after being filtered through a microfilter, and the independent vent pipe provides gas supply to a single dish, eliminating cross-contamination and significantly improving fermentation success rate and batch stability.
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Description

Technical Field

[0001] This invention relates to the field of mycelial fermentation culture, and specifically to a liquid fermentation culture device for morel mushrooms that facilitates mycelial inoculation. Background Technology

[0002] Morel liquid fermentation culture is an industrial technology that allows morel mycelium to rapidly suspend and grow and reproduce in a sterile liquid culture medium by precisely controlling conditions such as temperature, pH, and dissolved oxygen. Morels are strictly aerobic fungi, and liquid fermentation requires a continuous supply of sterile air. Oxygen supply is the core factor that determines the success or failure of fermentation.

[0003] In patent application CN221807680U, published on 2024-10-08, entitled "A Morel Mycelium Culture Dish for Easy Observation," this application discloses a morel mycelium culture dish for easy observation. It includes a culture dish base and a culture dish lid that can rotate relative to each other; a culture cavity for placing culture medium is formed inside the culture dish base, and a transparent cover portion is formed above the culture cavity on the culture dish lid; a graduated layer is provided on the cover portion, including a graduation line passing through the center of rotation of the cover portion and a reading indicator line perpendicular to the graduation line, with graduation readings provided in the direction of the graduation line's extension; the reading indicator line includes two symmetrically arranged groups of indicator lines, the axis of symmetry of which passes through the center of rotation of the cover portion and is perpendicular to the graduation line. This invention can better achieve direct reading of the colony diameter during the cultivation of morel mycelium.

[0004] In the aforementioned patents or prior art, the liquid fermentation culture of morel mushrooms requires placing a culture container filled with sterile liquid culture medium in a constant-temperature incubator, and precisely controlling environmental parameters to achieve mycelial proliferation. To prevent contamination by other microorganisms, the culture system must maintain a strictly sterile isolation state, avoiding direct contact with the unsterilized external environment. However, during the culture process, the inoculation operation requires introducing pure strains into a sealed liquid culture medium; morel mushrooms are strictly aerobic fungi, and mycelial growth depends entirely on a continuous oxygen supply. Both of these key steps inevitably require the culture system to exchange substances with the outside air, which provides a channel for the invasion of other microorganisms, easily causing contamination of the culture system, leading to abnormal mycelial growth or even fermentation failure.

[0005] Therefore, it is necessary to invent a liquid fermentation culture device for morel mushrooms that facilitates mycelial inoculation to solve the above problems. Summary of the Invention

[0006] The purpose of this invention is to provide a liquid fermentation culture device for morel mushrooms that facilitates mycelial inoculation. By designing a sealed channel structure that integrates inoculation and ventilation, it integrates double opposing independent sealing, online disinfection of inoculation tools, and automated batch aseptic ventilation functions. This solves the problems of existing liquid fermentation culture of morel mushrooms, such as the need to open the lid for inoculation leading to a high risk of large-area contamination, easy introduction of exogenous bacteria by inoculation tools, separate openings for ventilation and inoculation increasing contamination sites, easy cross-contamination between units in batch culture, and cumbersome operation and low degree of automation.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a liquid fermentation culture device for morel mushrooms that facilitates mycelial inoculation, comprising a cabinet door rotatably connected to one side of the opening of a fermentation culture cabinet, a support partition sequentially slidably connected inside the fermentation culture cabinet, multiple sets of placement cylinders arrayed on each set of support partitions, a culture dish placed in each set of placement cylinders, a lid fitted onto each set of culture dishes, a through cylinder penetrating through each set of lids, and the interior of the through cylinder communicating with the space above the lid and the interior of the culture dish, respectively, an annular groove being formed inside the through cylinder, with the opening of the annular groove facing upwards from the lid, and a push cylinder penetrating through the annular groove, with the interior of the push cylinder communicating with the interior space of the through cylinder.

[0008] As a preferred embodiment of the present invention, a conical cylinder is installed above the through cylinder, and the conical cylinder communicates with the internal space of the through cylinder, and the conical cylinder is located inside the push cylinder.

[0009] As a preferred embodiment of the present invention, limit blocks are symmetrically installed on the lower outer side of the push cylinder, and limit grooves are symmetrically opened on the inner wall of the annular groove, and the limit grooves are slidably connected with the corresponding limit blocks. A sealing ring is installed at the edge of the push cylinder opening, and the sealing ring is in contact with the inner wall of the annular groove.

[0010] As a preferred embodiment of the present invention, a limiting ring is fixedly sleeved on the upper outer side of the push cylinder, and a spring is sleeved on the push cylinder, with the two ends of the spring respectively fitting against the limiting ring and the surface of the cover.

[0011] As a preferred embodiment of the present invention, a plurality of trapezoidal plates are rotatably connected in a ring shape on the lower part of the inner wall of the push cylinder, and each group of trapezoidal plates is in contact with each other and in contact with the inclined surface of the conical cylinder. A conical rubber sealing gasket is installed on the surface of each group of trapezoidal plates inside the push cylinder, and the conical rubber sealing gasket does not completely cover the protruding connection of each group of trapezoidal plates.

[0012] As a preferred embodiment of the present invention, multiple sets of trapezoidal plates II are rotatably connected in a ring on the upper part of the inner wall of the push cylinder, and each set of trapezoidal plates II is in contact with each other, and the protrusions formed by the trapezoidal plates II are opposite to the protrusions formed by the trapezoidal plates I.

[0013] As a preferred embodiment of the present invention, each group of trapezoidal plates two has an inclined surface on the side away from the inside of the push cylinder, and the inclined surfaces of each group are combined to form a conical groove. A conical rubber sealing cylinder is fixedly installed between the two groups of trapezoidal plates two on the side of the inclined surface, and the inside of the conical rubber sealing cylinder is in communication with the inside space of the conical groove formed by the inclined surface.

[0014] As a preferred embodiment of the present invention, the upper part of the conical rubber sealing cylinder is fixedly connected to the edge of the inner wall of the push cylinder, and the bottom of the conical rubber sealing cylinder is provided with an annular holding groove, which is located at the connection point with the conical groove formed by the inclined surface.

[0015] As a preferred embodiment of the present invention, guide rods are symmetrically installed on the inner wall of the fermentation culture cabinet, and a support frame is slidably connected between each group of guide rods. Electric push rods are symmetrically installed between the support frame and the inner wall of the fermentation culture cabinet, and three groups of support frames are arranged sequentially inside the fermentation culture cabinet, with each group of support frames located above the corresponding support partition.

[0016] As a preferred embodiment of the present invention, multiple sets of miniature air filters are installed in an array above the support frame, and each set of miniature air filters is connected by a ventilation pipe, with each set of ventilation pipes and the corresponding placement cylinder at the same vertical and horizontal position.

[0017] Compared with the prior art, the technical effects and advantages provided by the present invention in the above technical solution are as follows: This device achieves a basic seal on the culture dish through the lid, and a dynamic seal is formed by the sealing ring at the edge of the push tube opening and the inner wall of the annular groove, constructing a basic sterile barrier. The core adopts a double independent sealing structure composed of trapezoidal plate one and trapezoidal plate two. The entire inoculation process does not require opening the lid; the operation can be completed simply by moving the push tube up and down, completely avoiding the risk of large-area contamination caused by traditional open-lid inoculation. At the same time, the conical rubber sealing cylinder can elastically and tightly wrap the outer wall of the inoculation tube to achieve a dynamic seal, and the disinfectant in the annular groove at its bottom can wet and wipe the outer wall of the tube for disinfection, preventing the introduction of contaminants into the culture system with the inoculation tool from the source, and comprehensively ensuring the sterility and safety of the mycelial inoculation process. The electric push rod drives the support frame to move smoothly downwards along the guide rod, precisely inserting the venting tube into the corresponding push cylinder and ultimately penetrating the interior of the culture dish. Outside air must first pass through a micro-air filter to thoroughly remove airborne microbial spores and suspended particulate matter before entering the culture system through the venting tube. Simultaneously, the array of independently distributed venting tubes allows for individual air supply to each culture dish, eliminating cross-contamination between different culture units and significantly improving the success rate and batch stability of morel liquid fermentation culture. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this invention. For those skilled in the art, other drawings can be obtained based on these drawings.

[0019] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the internal structure of the fermentation incubator of the present invention; Figure 3 This is a schematic diagram of the slicing structure of the fermentation culture cabinet of the present invention; Figure 4 This is a schematic diagram of the overall structure of the petri dish of the present invention; Figure 5 This is a schematic diagram of the conical rubber sealing cylinder structure of the present invention; Figure 6 This is a schematic diagram of the overall slicing structure of the petri dish of the present invention; Figure 7 This is a schematic diagram of the combined structure of the conical rubber sealing cylinder and the trapezoidal plate of the present invention; Figure 8 This is a schematic diagram of the mating structure of the conical rubber sealing gasket and the trapezoidal plate of the present invention; Figure 9 This is a schematic diagram of the overall planed structure of the lid of the present invention; Figure 10 This is a schematic diagram of the support frame structure of the present invention; Figure 11 This is a schematic diagram of the placement cylinder layout structure of the present invention.

[0020] Explanation of reference numerals in the attached figures: 001. Fermentation and cultivation cabinet; 101. Cabinet door; 102. Supporting partition; 103. Placement cylinder; 201. Culture dish; 202. Lid; 203. Through cylinder; 204. Annular groove; 205. Conical cylinder; 206. Push cylinder; 207. Spring; 208. Limiting block; 209. Limiting groove; 210. Sealing ring; 211. Limiting ring; 301. Trapezoidal plate one; 302. Conical rubber sealing gasket; 303. Trapezoidal plate two; 304. Conical rubber sealing cylinder; 305. Annular holding groove; 306. Inclined surface; 401. Support frame; 402. Vent pipe; 403. Miniature air filter; 404. Guide rod; 405. Electric push rod. Detailed Implementation

[0021] To enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0022] This invention provides, for example Figure 1-11 The illustrated morel liquid fermentation culture device facilitates mycelial inoculation and includes a fermentation culture cabinet 001 with a door 101 rotatably connected to one side of the opening. Supporting partitions 102 are slidably connected inside the fermentation culture cabinet 001. Multiple placement cylinders 103 are arrayed on each set of supporting partitions 102, and each set of placement cylinders 103 contains a culture dish 201. A lid 202 is fitted onto each set of culture dishes 201. A through-tube 203 is connected through each set of lids 202, and the interior of the through-tube 203 communicates with the space above the lid 202 and the interior of the culture dish 201. An annular groove 204 is formed inside the through-tube 203, with the opening of the annular groove 204 facing upwards towards the lid 202. A pusher 206 is connected through the annular groove 204, and the interior of the pusher 206 communicates with the interior of the through-tube 203.

[0023] The sliding support partition 102 facilitates the batch handling and transfer of culture dishes 201, while the placement cylinder 103 precisely positions and limits the culture dishes 201 to prevent them from tipping over or shifting. The lid 202 provides a basic seal for the culture dishes 201. The through cylinder 203 constructs a unified channel for inoculation and ventilation, and the annular groove 204 provides a guide and limiting space for the sliding of the push cylinder 206, while also providing an installation base for the sealing structure. The push cylinder 206 serves as a shared execution carrier for inoculation and ventilation operations, allowing all operations to be completed without opening the lid 202, significantly reducing the risk of large-area contamination caused by opening the lid.

[0024] Furthermore, in the above structure, a conical cylinder 205 is installed above the through cylinder 203, and the conical cylinder 205 communicates with the internal space of the through cylinder 203, and the conical cylinder 205 is located inside the push cylinder 206.

[0025] The conical cylinder 205 can be used as a trigger for the first sealing valve. The sealing valve can be automatically opened and closed by pushing it with an inclined surface, without the need for an additional drive mechanism.

[0026] Furthermore, in the above structure, limit blocks 208 are symmetrically installed on the lower outer side of the push cylinder 206, and limit grooves 209 are symmetrically opened on the inner wall of the annular groove 204. The limit grooves 209 are slidably connected to the corresponding limit blocks 208. A sealing ring 210 is installed at the edge of the opening of the push cylinder 206, and the sealing ring 210 is in contact with the inner wall of the annular groove 204.

[0027] By cooperating with the limiting block 208 and the limiting groove 209, the vertical sliding stroke of the push cylinder 206 is limited, preventing the push cylinder 206 from coming out of the annular groove 204. At the same time, the circumferential rotation of the push cylinder 206 is completely restricted, ensuring the alignment accuracy of the upper and lower sealing structures. The sealing ring 210 achieves a dynamic seal between the outer wall of the push cylinder 206 and the inner wall of the annular groove 204, blocking outside air from entering the culture system through the gap between the two.

[0028] Furthermore, in the above structure, a limiting ring 211 is fixedly sleeved on the upper outer side of the push cylinder 206, and a spring 207 is sleeved on the push cylinder 206, with the two ends of the spring 207 respectively attached to the limiting ring 211 and the surface of the cover 202.

[0029] Spring 207 provides an upward reset force to push cylinder 206. After inoculation or ventilation is completed, push cylinder 206 can be automatically pushed back to the initial sealing position without manual reset, simplifying the operation process. Limiting ring 211 provides an upper support point for spring 207 and precisely limits the upper limit of push cylinder 206 reset, ensuring that the sealing structure can be completely closed.

[0030] Furthermore, in the above structure, multiple sets of trapezoidal plates 301 are rotatably connected in a ring shape on the lower part of the inner wall of the push cylinder 206, and each set of trapezoidal plates 301 is in contact with each other. Each set of trapezoidal plates 301 is in contact with the inclined surface of the conical cylinder 205. A conical rubber sealing gasket 302 is installed on the surface of each set of trapezoidal plates 301 inside the push cylinder 206, and the conical rubber sealing gasket 302 does not completely cover the protruding connection of each set of trapezoidal plates 301.

[0031] A sealing structure is formed by multiple sets of mutually fitting trapezoidal plates 301. In the initial state, it is tightly fitted with the inclined surface of the conical cylinder 205, blocking the inoculation ventilation channel. When the push cylinder 206 moves downward, the trapezoidal plates 301 are pushed outward by the inclined surface of the conical cylinder 205 and open, allowing the channel to be opened. The conical rubber sealing gasket 302 enhances the surface sealing effect between the trapezoidal plates 301 and the conical cylinder 205, and can also reset the trapezoidal plates 301 afterward. The not fully covered protruding connection can ensure that the trapezoidal plates 301 can rotate smoothly and avoid the sealing gasket interfering with its opening and closing action.

[0032] Furthermore, in the above structure, multiple sets of trapezoidal plates 303 are rotatably connected in a ring on the upper part of the inner wall of the push cylinder 206, and each set of trapezoidal plates 303 fits into each other, and the protrusions formed by the trapezoidal plates 303 are arranged opposite to the protrusions formed by the trapezoidal plates 301.

[0033] The sealing structure is formed by multiple sets of interlocking trapezoidal plates 303, which together with trapezoidal plate 301 form a double independent sealing structure, greatly improving the sealing reliability of the channel. The protrusions on the opposite side allow the test tube for inoculating mycelium to push open trapezoidal plate 303 first when it is inserted. At the same time, the position of the push tube 206 remains unchanged under the action of spring 207. At this time, the sealing structure of trapezoidal plate 301 is in a closed state. When the test tube for inoculating mycelium is inserted, the sealing structure of trapezoidal plate 301 is opened by manually pressing the entire push tube 206, which in turn drives the test tube into the culture dish 201 for mycelial inoculation.

[0034] Furthermore, in the above structure, each group of trapezoidal plates 303 has an inclined surface 306 on the side away from the inside of the push cylinder 206, and the inclined surfaces 306 are combined to form a conical groove. A conical rubber sealing cylinder 304 is fixedly installed between each group of trapezoidal plates 303 on the side of the inclined surface 306, and the interior of the conical rubber sealing cylinder 304 is in communication with the interior space of the conical groove formed by the inclined surface 306.

[0035] The conical groove formed by the inclined surface 306 can guide the inoculated test tube to be accurately inserted into the center of the channel, avoiding splashing of the inoculation liquid. At the same time, the trapezoidal plate 303 rotates and opens synchronously. At this time, the conical rubber sealing cylinder 304 can elastically and tightly wrap the outer wall of the inoculated test tube, realizing a dynamic seal between the inoculated test tube and the channel, preventing outside air from entering the culture dish 201 with the test tube during the inoculation process.

[0036] Furthermore, in the above structure, the upper part of the conical rubber sealing cylinder 304 is fixedly connected to the inner wall edge of the push cylinder 206, and the bottom of the conical rubber sealing cylinder 304 is provided with an annular holding groove 305, and the annular holding groove 305 is located at the connection point of the conical groove formed by the inclined surface 306.

[0037] The fixed connection between the upper part of the conical rubber sealing cylinder 304 and the inner wall of the push cylinder 206 ensures that it can retract synchronously with the inoculation nozzle when it is pulled out, thereby driving the trapezoidal plate 303 to close automatically. The annular holding groove 305 can hold disinfectant. When the test tube is inserted, it will fit and wrap with the outer side of the conical rubber sealing cylinder 304. As the test tube continues to move downward, the edge of the fit will be concave downward, so that the disinfectant will adhere to the surface of the test tube. At the same time, the continuous downward movement will cause the wrapping part to wipe the surface of the test tube, thereby achieving the effect of disinfection and wiping.

[0038] Furthermore, in the above structure, guide rods 404 are symmetrically installed on the inner wall of the fermentation culture cabinet 001, and a support frame 401 is slidably connected between each set of guide rods 404. Electric push rods 405 are symmetrically installed between the support frame 401 and the inner wall of the fermentation culture cabinet 001. Three sets of support frames 401 are arranged in sequence inside the fermentation culture cabinet 001, and each set of support frames 401 is located above the corresponding support partition 102.

[0039] The guide rod 404 provides guidance for the up-and-down sliding of the support frame 401, ensuring its stability and verticality. The electric push rod 405 can drive the support frame 401 to move up and down precisely, realizing the automatic docking and separation of the venting pipe 402 and the push cylinder 206. The layered support frame 401 can correspond one-to-one with each layer of support partition 102, realizing synchronous ventilation of batch culture dishes 201, improving the automation level and production efficiency of the equipment. After the venting pipe 402 is inserted into the push cylinder 206, the continuous downward pressure of the support frame 401 will push the push cylinder 206 to move down, thereby opening the trapezoidal plate 301. In addition, during the culture process, the venting pipe 402 can remain in the sealed structure formed by the trapezoidal plate 303 inside the push cylinder 206 unless necessary, so the opening and closing of the trapezoidal plate 301 can be controlled independently.

[0040] Furthermore, in the above structure, multiple sets of miniature air filters 403 are arranged in an array above the support frame 401, and each set of miniature air filters 403 is connected by a ventilation pipe 402, and each set of ventilation pipes 402 and the corresponding placement cylinder 103 are at the same vertical and horizontal position.

[0041] The micro air filter 403 can efficiently filter the air entering the culture dish 201, removing bacteria and particulate matter from the air and ensuring the absolute sterility of the air supply; the vent pipe 402 can be precisely connected to the push tube 206 to directly introduce the filtered sterile air into the culture dish 201, meeting the aerobic requirements for morel mycelial growth; the array of independent vent pipes 402 can achieve independent ventilation for each culture dish 201, completely avoiding cross-contamination between different culture units.

[0042] like Figure 1-11 As shown, when mycelial culture is required, the culture medium can be put into the culture dish 201 and the lid 202 can be closed. At this time, the pusher 206 is in the highest reset position under the upward elastic force of the spring 207 sleeved on its outside, so that the multiple sets of trapezoidal plates 301 and 303 inside the pusher 206 form a seal, isolating the culture dish 201 from the outside world. During mycelial inoculation, disinfectant solution needs to be pre-placed in the annular container 305. Simultaneously, the inoculation tube containing morel mycelium is inserted into the conical groove above the pusher 206. The inclined surface 306 guides the tube to precisely align with the center of the channel. As the tube moves downwards, it first pushes open the two trapezoidal plates 303, simultaneously causing the conical rubber sealing cylinder 304 to stretch and deform downwards. At this point, the elastic force of the spring 207 is greater than the downward pressure of the tube, and the pusher 206 remains in its position, keeping the trapezoidal plate 301 closed, preventing outside air from entering. The tube enters the culture dish 201, while the conical rubber sealing cylinder 304 elastically and tightly wraps around the outer wall of the tube, achieving a dynamic seal between the tube and the channel. As the tube continues to be inserted downwards, the disinfectant in the annular holding groove 305 will wet the outer wall of the tube, and the wrapping edge of the conical rubber sealing cylinder 304 will wipe and disinfect the surface of the tube, further blocking the possibility of introducing bacteria with the tube. Continue to press the tube downwards to move it to a position above the trapezoidal plate 301 without contacting it, leaving a certain amount of extra space. At this point, manually press the push cylinder 206 to compress the spring 207 and slide it downwards along the annular groove 204. The limiting block 208 moves downwards along the limiting groove 209 to ensure smooth movement without circumferential rotation. During the downward movement of the push cylinder 206, the trapezoidal plate 301 slides relative to the inclined surface of the conical cylinder 205 and rotates outwards under the push of the inclined surface, opening the channel and connecting it with the interior of the culture dish 201. The test tube then extends into the liquid culture medium in the culture dish 201 to complete the mycelial inoculation. After inoculation, the push cylinder 206 can be reset under the action of the spring 207. At this time, the trapezoidal plate 301 automatically resets and closes under the elastic force of the conical rubber sealing gasket 302. Then, slowly pull the test tube upwards. At the same time, the conical rubber sealing cylinder 304 contracts synchronously with the test tube, causing the trapezoidal plate 303 to automatically reset and close. The entire inoculation process does not require opening the lid 202. The operation is completed only by the up-and-down movement of the push cylinder 206 and the sequential opening and closing of the two sealing structures.

[0043] After mycelial inoculation, the culture dish 201 is placed into the placement cylinder 103, and the cabinet door 101 is closed. During the cultivation process, the electric push rod 405 on the inner wall of the fermentation culture cabinet 001 is activated. The electric push rod 405 drives the support frame 401 to move smoothly downward along the guide rod 404, so that the venting pipes 402 distributed in the array below the support frame 401 are precisely aligned and inserted into the corresponding push cylinder 206. The venting pipes 402 push down to open the trapezoidal plate 2 303. At this time, the push cylinder 206 still maintains its initial position, and the trapezoidal plate 301 is in a closed state. The support frame 401 continues to press down, eventually pushing the push cylinder 206 downward, which in turn drives the trapezoidal plate 301 to open under the action of the conical cylinder 205, allowing the venting pipes 402 to open. The air is connected to the interior of the culture dish 201. At this time, the outside air is efficiently filtered by the micro air filter 403 above the support frame 401 to remove bacteria and particulate matter, and then directly introduced into the interior of the culture dish 201 through the vent pipe 402 to meet the oxygen requirements for the growth of morel mycelium. The array of independent vent pipes 402 enables independent ventilation of each culture dish 201, completely avoiding cross-contamination between different culture units. During the entire culture process, the vent pipe 402 can remain connected to the push cylinder 206. By controlling the extension and retraction of the electric push rod 405, the opening and closing of the trapezoidal plate 301 can be independently controlled to achieve precise ventilation as needed, effectively isolating external bacteria throughout the process and ensuring the smooth progress of the liquid fermentation culture of morel.

[0044] The foregoing has only described certain exemplary embodiments of the present invention by way of illustration. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the foregoing drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A liquid fermentation culture device for morel mushrooms that facilitates mycelial inoculation, comprising a fermentation culture cabinet (001) with a cabinet door (101) rotatably connected to one side of the opening, wherein supporting partitions (102) are sequentially slidably connected inside the fermentation culture cabinet (001), and multiple sets of placement cylinders (103) are arrayed on each set of supporting partitions (102), characterized in that: Each group of placement tubes (103) contains a culture dish (201), and each group of culture dishes (201) is fitted with a lid (202). Each group of lids (202) is connected to a through tube (203), and the inside of the through tube (203) is connected to the space above the lid (202) and the inside of the culture dish (201). The inside of the through tube (203) is provided with an annular groove (204), and the opening of the annular groove (204) faces the top of the lid (202). A push tube (206) is connected to the annular groove (204), and the inside of the push tube (206) is connected to the space inside the through tube (203).

2. The morel liquid fermentation culture equipment for easy mycelial inoculation according to claim 1, characterized in that: A conical cylinder (205) is installed above the through cylinder (203), and the conical cylinder (205) communicates with the internal space of the through cylinder (203), and the conical cylinder (205) is located inside the push cylinder (206).

3. The morel liquid fermentation culture equipment for easy mycelial inoculation according to claim 2, characterized in that: Limiting blocks (208) are symmetrically installed on the lower outer side of the push cylinder (206). Limiting grooves (209) are symmetrically opened on the inner wall of the annular groove (204), and the limiting grooves (209) are slidably connected to the corresponding limiting blocks (208). A sealing ring (210) is installed at the edge of the opening of the push cylinder (206), and the sealing ring (210) is in contact with the inner wall of the annular groove (204).

4. The morel liquid fermentation culture equipment for easy mycelial inoculation according to claim 3, characterized in that: A limiting ring (211) is fixedly sleeved on the upper outer side of the push cylinder (206), and a spring (207) is sleeved on the push cylinder (206), with the two ends of the spring (207) respectively attached to the surface of the limiting ring (211) and the cover (202).

5. The morel liquid fermentation culture equipment for easy mycelial inoculation according to claim 4, characterized in that: The inner wall of the push cylinder (206) is rotatably connected in a ring shape with multiple sets of trapezoidal plates (301), and each set of trapezoidal plates (301) is in contact with each other. Each set of trapezoidal plates (301) is in contact with the inclined surface of the conical cylinder (205). Each set of trapezoidal plates (301) is equipped with a conical rubber sealing gasket (302) on the surface inside the push cylinder (206), and the conical rubber sealing gasket (302) does not completely cover the protruding connection of each set of trapezoidal plates (301).

6. The morel liquid fermentation culture equipment for easy mycelial inoculation according to claim 5, characterized in that: The push cylinder (206) has multiple sets of trapezoidal plates (303) arranged in a ring on the upper part of its inner wall. Each set of trapezoidal plates (303) fits into each other, and the protrusions formed by the trapezoidal plates (303) are opposite to the protrusions formed by the trapezoidal plates (301).

7. The morel liquid fermentation culture equipment for easy mycelial inoculation according to claim 6, characterized in that: Each trapezoidal plate 2 (303) of each group has an inclined surface (306) on the side away from the inside of the push cylinder (206), and the inclined surfaces (306) of each group are combined to form a conical groove. A conical rubber sealing cylinder (304) is fixedly installed between the two sides of the inclined surface (306) of each group of trapezoidal plates 2 (303), and the interior of the conical rubber sealing cylinder (304) is connected to the interior space of the conical groove formed by the inclined surface (306).

8. The morel liquid fermentation culture equipment for easy mycelial inoculation according to claim 7, characterized in that: The conical rubber sealing cylinder (304) is fixedly connected to the inner wall edge of the push cylinder (206) at the top. The bottom of the conical rubber sealing cylinder (304) is provided with an annular holding groove (305), and the annular holding groove (305) is located at the connection point of the conical groove formed by the inclined surface (306).

9. The morel liquid fermentation culture equipment for easy mycelial inoculation according to claim 1, characterized in that: Guide rods (404) are symmetrically installed on the inner wall of the fermentation culture cabinet (001). A support frame (401) is slidably connected between each group of guide rods (404). Electric push rods (405) are symmetrically installed between the support frame (401) and the inner wall of the fermentation culture cabinet (001). There are three groups of support frames (401) arranged in sequence in the fermentation culture cabinet (001), and each group of support frames (401) is located above the corresponding support partition (102).

10. The morel liquid fermentation culture equipment for easy mycelial inoculation according to claim 9, characterized in that: Multiple sets of miniature air filters (403) are installed in an array above the support frame (401). Each set of miniature air filters (403) has a ventilation pipe (402) installed underneath it, and each set of ventilation pipes (402) is at the same vertical and horizontal position as the corresponding placement cylinder (103).

Citation Information

Patent Citations

  • Morchella hypha culture dish suitable for observation

    CN221807680U