A POCT card holder

Abstract

This invention belongs to the field of nucleic acid detection technology, specifically relating to a POCT cartridge, including a reaction tube with a first sealing film at its right end; a storage tube, which includes a receiving tube placed inside the reaction tube; a detection tube, which includes a sliding rod connected to a first puncture head, with a channel in the inner cavity of the sliding rod and a viewing window above the sliding rod; a fixing part, which includes a mounting block connected to a second puncture head, with a test strip connected to its right side; and a limiting tube, which includes a sleeve fitted around the outer ring of the sliding rod and the mounting block, with a fixing tube connected to its left end, fitted around the outer ring of the reaction tube. This invention enables the entire RPA detection process to be completed without opening the lid, avoiding aerosol contamination affecting the detection results and preventing damage to the surrounding environment. It also allows for separate storage and rapid installation of the device, making operation simple and fast, and avoiding errors.

Description

Technical Field

[0001] This invention belongs to the field of nucleic acid detection technology, specifically relating to a POCT cartridge. Background Technology

[0002] Recombinase polymerase amplification (RPA) is an isothermal nucleic acid amplification technique that can rapidly amplify target nucleic acids without the need for thermal cycling instruments. Combining RPA with lateral flow spectroscopy (LFD) strips enables visual detection of amplified products, offering advantages such as ease of operation and rapid detection. It is considered a powerful technique suitable for rapid on-site testing. However, existing RPA-LFD detection methods have significant shortcomings in practical applications.

[0003] First, after the RPA amplification reaction is complete, the amplification product needs to be removed from the container and added to a lateral chromatography test strip. This opening operation can easily cause the amplification product to form an aerosol and diffuse into the environment, resulting in cross-contamination in the laboratory and false positive results. This severely restricts the widespread application of this technology in non-professional environments such as clinical settings.

[0004] Secondly, the existing operating procedures are cumbersome: they involve multiple independent steps such as sample addition, amplification reaction, product dilution, buffer mixing and test strip addition, requiring repeated transfer of liquid between multiple containers, which requires a high level of proficiency from the operators. Furthermore, the operation steps are scattered, and improper operation by personnel can easily affect the accuracy of the test results. Summary of the Invention

[0005] The purpose of this invention is to provide a POCT cartridge that enables the entire RPA testing process to be completed without opening the lid, avoiding aerosol contamination that could affect the test results and prevent damage to the surrounding environment. It also allows for separate storage and rapid installation of the device, making the operation simple and quick and avoiding errors.

[0006] The specific technical solution adopted by this invention is as follows: A POCT card holder, comprising: The reaction tube has a first sealing membrane installed at its right end. The storage tube includes a receiving tube, which is placed inside the reaction tube. The detection tube includes a sliding rod, which is located on the right side of the reaction tube. A first puncture head is connected to the left side of the sliding rod. A channel is opened in the inner cavity of the sliding rod, and a viewing window is provided above the sliding rod. The fixing part includes a mounting block connected to the left end of the sliding rod. A second puncture head is connected to the left side of the mounting block, and a test strip is connected to the right side. The limiting tube includes a sleeve that is fitted around the outer ring of the sliding rod and the mounting block. A fixing tube is connected to the left end of the sleeve and is fitted around the outer ring of the reaction tube. The sliding rod slides along the sleeve, causing the first and second puncture heads to pierce the receiving tube, resulting in the liquid in the receiving tube mixing with the liquid in the reaction tube. Then the device is inverted to drive the mixed liquid into the channel to react with the test paper.

[0007] In a preferred embodiment, a reaction chamber is provided on the left side of the inner cavity of the reaction tube, and a receiving chamber is provided on the right side of the inner cavity. The outer ring of the receiving tube is movably fitted into the receiving chamber, and a first sealing membrane is fixedly connected to the outer ring of the right end of the reaction tube.

[0008] In a preferred embodiment, a magnetic stirring rod is placed inside the reaction chamber, and first buckles are fixedly connected to the upper and lower sides of the right end of the outer ring of the reaction tube.

[0009] In a preferred embodiment, the storage tube is placed inside the storage cavity, and a second sealing film is fixedly connected to both the front and rear ends of the storage tube.

[0010] In a preferred embodiment, the sliding rod is located at the right end of the reaction tube and the receiving tube. A first puncture head is fixedly connected to the lower side of the sliding rod facing the reaction tube. The inner cavity of the sliding rod has a channel with two sets of outlets, both of which are located at the left end of the sliding rod and are distributed vertically.

[0011] In a preferred embodiment, a second slot is provided at the upper end and the left end of the sliding rod, a convex ring is fixedly connected to the middle of the outer ring of the sliding rod, and a through groove is provided at the position between the two sets of convex rings on the outer ring of the sliding rod, and the through groove is connected to the channel, and a viewing window is fixedly connected in the through groove.

[0012] In a preferred embodiment, a guide block is fixedly connected to the lower right end of the sliding rod, a cavity is opened at the upper right side of the outer ring of the sliding rod, and a trapezoidal block is slidably connected in the cavity. A spring is fixedly connected to the lower end of the trapezoidal block, and the lower end of the spring is fixedly connected to the bottom of the cavity of the sliding rod.

[0013] In a preferred embodiment, the mounting block is movably inserted into the upper left side of the sliding rod. A second puncture head is fixedly connected to the left end of the mounting block, and the second puncture head is positioned above the first puncture head. A clamping plate is fixedly connected to the right end of the mounting block, and a test strip is held in the middle of the clamping plate. Both the clamping plate and the test strip are movably inserted into the upper end of the channel cavity. Second buckles are fixedly connected to the right side and lower end of the mounting block, and the second buckles are engaged in the second slots.

[0014] In a preferred embodiment, the sleeve is movably fitted onto the outer ring of the sliding rod and the mounting block. A fixed tube is connected to the left end of the sleeve, and the fixed tube is movably fitted onto the outer ring of the reaction tube. A first slot is provided at the right end of the inner cavity of the fixed tube, and a first buckle is engaged with the inner cavity of the first slot. A through groove is provided at the upper end of the outer ring of the sleeve, and an observation plate is fixedly connected in the through groove. The observation plate is positioned above the viewing window.

[0015] In a preferred embodiment, two sets of grooves are provided in the middle of the inner cavity of the sleeve, and a convex ring is engaged in the groove. A guide groove is provided in the inner cavity at the right end of the sleeve, and a guide block is slidably connected in the guide groove. A mating groove is provided on the upper right side of the inner cavity of the sleeve, and the mating groove is located on the left side of the trapezoidal block.

[0016] The technical effects achieved by this invention are as follows: The reaction tube, storage tube, and detection tube of this invention enable the entire RPA detection process to be completed without opening the cap, avoiding aerosol contamination that could affect the detection results and also preventing damage to the surrounding environment. In use, the sample and the storage tube are connected inside the sleeve and the fixing tube, and the sample is punctured by the puncture head to mix the treatment solution with the sample. After mixing, the device is inverted to allow the mixed sample to flow into the channel and contact the test strip, completing the entire detection process without opening, thus ensuring sealing and safety. The reaction tube, storage tube, detection tube, and fixing part of the present invention enable separate storage and quick installation of the device, making operation simple and fast and avoiding errors. Before testing, the reaction liquid and the treatment liquid are simply filled into the reaction tube and the storage tube for storage. When testing is required, they can be directly assembled and punctured without any extra steps, which greatly simplifies the testing process. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is an overall exploded view of the present invention; Figure 3 This is a schematic cross-sectional view of the entire invention. Figure 4 This is a cross-sectional view of the reaction tube and the receiving tube in this invention. Figure 5 This is a cross-sectional schematic diagram of the detection tube in this invention; Figure 6 This is a schematic diagram showing the disassembly of the detection tube and the sleeve in this invention; Figure 7 This is a schematic diagram of the trapezoidal block in this invention; Figure 8 This is a schematic diagram of the connection between the detection tube and the fixing part in this invention; Figure 9 This is a cross-sectional schematic diagram of the fixing part in this invention; Figure 10 This is a cross-sectional view of the detection tube and the fixing part in this invention; Figure 11 This is a schematic diagram showing the positions of the first puncture head and the second puncture head in this invention.

[0018] The attached diagram lists the components represented by each number as follows: 10. Reaction tube; 11. Reaction chamber; 12. Storage chamber; 13. First sealing membrane; 14. Magnetic stirring rod; 15. First snap fastener; 20. Storage tube; 21. Storage tube; 22. Second sealing membrane; 30. Detection tube; 31. Sliding rod; 32. First puncture head; 33. Channel; 34. Second slot; 35. Protruding ring; 36. Viewing window; 37. Guide block; 38. Trapezoidal block; 39. Spring; 40. Fixing part; 41. Mounting block; 42. Second puncture head; 43. Clamping plate; 44. Test paper; 45. Second snap fastener; 50. Limiting tube; 51. Sleeve; 52. Fixing tube; 53. First slot; 54. Observation plate; 55. Groove; 56. Guide groove; 57. Docking groove. Detailed Implementation

[0019] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0020] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0021] Secondly, the term "an embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in a preferred embodiment" appearing in different places throughout this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that mutually excludes other embodiments.

[0022] Secondly, the present invention is described in detail with reference to the schematic diagrams. When detailing the embodiments of the present invention, for ease of explanation, the cross-sectional views illustrating the device structure may be partially enlarged, not according to the usual scale. Furthermore, the schematic diagrams are merely examples and should not limit the scope of protection of the present invention. In addition, actual fabrication should include three-dimensional spatial dimensions of length, width, and depth.

[0023] Please see the appendix Figures 1 to 11 As shown, this embodiment provides a POCT card cartridge, including: The reaction tube 10 has a first sealing membrane 13 at its right end; Storage tube 20, which includes receiving tube 21, is placed inside the reaction tube 10; The detection tube 30 includes a sliding rod 31, which is located on the right side of the reaction tube 10. A first puncture head 32 is connected to the left side of the sliding rod 31. A channel 33 is opened in the inner cavity of the sliding rod 31, and a viewing window 36 is provided above the sliding rod 31. The fixing part 40 includes a mounting block 41, which is connected to the left end of the sliding rod 31. The second puncture head 42 is connected to the left side of the mounting block 41, and the test strip 44 is connected to the right side. Limiting tube 50 includes a sleeve 51, which is sleeved on the outer ring of sliding rod 31 and mounting block 41. The left end of sleeve 51 is connected to fixing tube 52, which is sleeved on the outer ring of reaction tube 10. The sliding rod 31 slides along the sleeve 51, causing the first puncture head 32 and the second puncture head 42 to puncture the receiving tube 21, causing the liquid in the receiving tube 21 to mix with the liquid in the reaction tube 10. Then the device is inverted, causing the mixed liquid to flow into the channel 33 and react with the test paper 44.

[0024] It should be noted that, in order to ensure the stable use of the device, it needs to be used in conjunction with a magnetic stirrer to drive the liquid in the reaction tube 10 to mix quickly.

[0025] In this embodiment, the first sealing film 13 is opened during use, the nucleic acid sample is placed into the reaction tube 10, and then the receiving tube 21 is placed into the reaction tube 10. The reaction tube 10 is then inserted into the fixing tube 52 for fixation. After clamping the test strip 44 on the right side of the mounting block 41, the mounting block 41 is connected and fixed to the sliding rod 31, and the test strip 44 is inserted into the channel 33. The sliding rod 31 is then inserted into the sleeve 51 and gradually pushed, causing the first puncture head 32 and the second puncture head 42 to puncture the receiving tube 21, causing the liquid inside to mix with the nucleic acid sample. After the mixed liquid in the reaction tube 10 has reacted for a period of time, the device is inverted, allowing the mixed liquid to flow downwards and into the channel 33 through the guidance of the two sets of puncture heads, contacting the test strip 44. The reaction of the test strip 44 can then be observed through the viewing window 36.

[0026] Secondly, please refer to it again. Figures 1 to 4 The reaction tube 10 has a reaction chamber 11 on the left side and a receiving chamber 12 on the right side. The outer ring of the receiving tube 21 is movably sleeved in the receiving chamber 12. The outer ring of the right end of the reaction tube 10 is fixedly connected to the first sealing membrane 13. A magnetic stirring rod 14 is placed inside the reaction chamber 11, and the upper and lower sides of the right end of the outer ring of the reaction tube 10 are fixedly connected with the first buckle 15.

[0027] It should be noted that a lyophilized reaction solution bulb is placed inside reaction tube 10 for subsequent testing; The first buckle 15 is made of plastic and has a cavity at the bottom for subsequent connection.

[0028] In this embodiment, when in use, the first sealing membrane 13 is opened, the nucleic acid sample is added into the reaction chamber 11, and then the receiving tube 21 is inserted into the receiving chamber 12 to complete the initial assembly of the device.

[0029] Secondly, please refer to it again. Figures 2 to 4 The storage tube 21 is placed inside the storage cavity 12, and the front and rear ends of the storage tube 21 are fixedly connected with the second sealing film 22.

[0030] It should be noted that the receiving tube 21 is filled with processing solution, which is used to mix with the reaction solution and nucleic acid sample in the reaction tube 10 later; Both the first sealing film 13 and the second sealing film 22 are aluminum foil heat-sealing films; During the preparation process, the reaction chamber 11 should first be filled with the freeze-dried reaction solution ball and the magnetic stirring rod 14. Then, the reaction tube 10 should be sealed with the first sealing membrane 13. After filling the receiving tube 21 with the treatment solution, the receiving tube 21 should be sealed with the second sealing membrane 22.

[0031] In this embodiment, during use, the receiving tube 21 is placed inside the receiving cavity 12 and fixed by the inner wall of the receiving cavity 12 to prevent the receiving tube 21 from shaking or shifting inside the reaction tube 10, thus ensuring that the puncture operation can be accurately aligned with the receiving tube 21 to complete the membrane breaking.

[0032] Secondly, please refer to it again. Figure 3 , Figures 5 to 7 The sliding rod 31 is located at the right end of the reaction tube 10 and the receiving tube 21. The first puncture head 32 is fixedly connected to the lower side of the sliding rod 31 facing the reaction tube 10. The inner cavity of the sliding rod 31 is provided with a channel 33. The channel 33 is provided with two sets of outlets, and both sets of outlets are located at the left end of the sliding rod 31, distributed vertically. The upper and left ends of the sliding rod 31 are provided with second slots 34. The middle of the outer ring of the sliding rod 31 is fixedly connected with a protruding ring 35. The outer ring of the sliding rod 31 is provided with a through groove in the middle of the two sets of protruding rings 35. The through groove is connected to the channel 33. A viewing window 36 is fixedly connected in the through groove. A guide block 37 is fixedly connected to the lower right end of the sliding rod 31. A cavity is opened at the upper right side of the outer ring of the sliding rod 31, and a trapezoidal block 38 is slidably connected in the cavity. A spring 39 is fixedly connected to the lower end of the trapezoidal block 38, and the lower end of the spring 39 is fixedly connected to the bottom of the cavity of the sliding rod 31.

[0033] It should be noted that the outer diameter of the first puncture head 32 is adapted to the inner diameter of the receiving tube 21 and the reaction chamber 11, so that when the sliding rod 31 slides, it can drive the first puncture head 32 to directly pass through the receiving tube 21 and enter the reaction chamber 11, so that the liquid in the receiving tube 21 can enter the reaction chamber 11. The channel 33 has a U-shaped structure, and the entrance of the channel 33 is located at the inner ring of the first puncture head 32, so that when the device is inverted, the liquid in the reaction tube 10 can flow into the channel 33 along the inner ring of the first puncture head 32. The upper left and right sides of the trapezoidal block 38 are provided with ramps, which can be used to squeeze the trapezoidal block 38 into the inner cavity of the sliding rod 31.

[0034] In this embodiment, when the sliding rod 31 is pushed along the sleeve 51 towards the receiving tube 21, the guide block 37 slides along the guide groove 56 to ensure that the sliding rod 31 does not rotate circumferentially, so that the first puncture head 32 and the second puncture head 42 are always aligned with the corresponding positions of the receiving tube 21, and the puncture operation is completed smoothly. When the sliding rod 31 moves into place, the trapezoidal block 38 will pop out under the elastic force of the spring 39 and lock into the docking groove 57 to lock the position of the sliding rod 31, prevent the sliding rod 31 from retracting, and ensure that the liquid mixing and subsequent detection process proceed stably.

[0035] Please refer to it again. Figures 8 to 11 The mounting block 41 is movably inserted into the upper left side of the sliding rod 31. The left end of the mounting block 41 is fixedly connected to the second puncture head 42, which is positioned above the first puncture head 32. The right end of the mounting block 41 is fixedly connected to the clamping plate 43, which holds the test strip 44 in the middle. Both the clamping plate 43 and the test strip 44 are movably inserted into the upper end of the inner cavity of the channel 33. The right side and lower end of the mounting block 41 are fixedly connected to the second buckle 45, which are both engaged in the second slot 34.

[0036] It should be noted that the inner ring of the second puncture head 42 is provided with an arc-shaped ramp, which is designed to guide the liquid flowing down when the device is inverted into the inlet of the channel 33 through the ramp. The clamp 43 has a U-shaped structure and an anti-slip pad on the inner ring, which is designed to ensure the stability of clamping the test paper 44. The test strip 44 is a colloidal gold test strip, and the end that contacts the sample extends into the inner cavity of the channel 33. The side that displays the test results is set at the position of the perspective window 36 for easy observation later. The mounting block 41 blocks and seals the outlet above the channel 33 to prevent sample liquid from covering the surface of the test strip 44, thus affecting the accuracy of the test and the observation effect.

[0037] In this embodiment, the test strip 44 is first clamped and fixed inside the clamping plate 43. Then, the mounting block 41 is aligned with the insertion position on the upper left side of the sliding rod 31 and pushed in, so that the second buckle 45 is correspondingly inserted into the second slot 34, completing the pre-assembly with the sliding rod 31. At this time, the clamping plate 43 and the test strip 44 enter the upper end of the channel 33 together. The result display area of ​​the test strip 44 is aligned with the viewing window 36. The second puncture head 42 protrudes from the left end of the sliding rod 31 and is aligned vertically with the first puncture head 32 with the storage tube 21, preparing for subsequent puncture operations.

[0038] Please refer to it again. Figures 2 to 7 The sleeve 51 is movably sleeved on the outer ring of the sliding rod 31 and the mounting block 41. The left end of the sleeve 51 is connected to the fixing tube 52, which is movably sleeved on the outer ring of the reaction tube 10. The right end of the inner cavity of the fixing tube 52 is provided with a first slot 53, and the first buckle 15 is engaged in the inner cavity of the first slot 53. The upper end of the outer ring of the sleeve 51 is provided with a through groove, and an observation plate 54 is fixedly connected in the through groove. The observation plate 54 is set above the viewing window 36. Two sets of grooves 55 are provided in the middle of the inner cavity of the sleeve 51, and the protruding ring 35 is engaged in the groove 55. A guide groove 56 is provided in the inner cavity of the right end of the sleeve 51, and the guide block 37 is slidably connected in the guide groove 56. A docking groove 57 is provided on the upper right side of the inner cavity of the sleeve 51, and the docking groove 57 is located on the left side of the trapezoidal block 38.

[0039] It should be noted that both the first clip 15 and the second clip 45 are made of plastic and have cavities at their lower ends. This allows the clips to be pressed into the cavities first and then popped out when aligned with the slots to complete the snap-fit ​​fixing. The convex ring 35 is made of a flexible material that can be squeezed and folded. The left and right sides of the sliding rod 31 where the convex ring 35 is installed should be provided with annular grooves with a narrow diameter, so that when the sliding rod 31 is inserted into the sleeve 51, the convex ring 35 can be squeezed and fitted into the annular groove, thus avoiding affecting the connection of the sliding rod 31. Both the observation plate 54 and the viewing window 36 are made of transparent material (such as plastic), which allows for direct observation of the test strip 44 without opening the device or touching the test strip 44. The distance between the docking groove 57 and the right end of the sleeve 51 is the same as the length of the sliding rod 31 inserted into the reaction tube 10, so as to prevent the sliding rod 31 from falling off when the tube is inverted after it is fully inserted into the reaction tube 10, which would cause sample contamination. The length of the guide groove 56 is greater than the distance between the mating groove 57 and the right end of the sleeve 51, so as to avoid affecting the sliding of the sliding rod 31.

[0040] In this embodiment, during use, the reaction tube 10 is first inserted into the fixing tube 52, and the first buckle 15 is engaged in the first slot 53, completing the fixed connection between the reaction tube 10 and the limiting tube 50. At this time, the reaction tube 10 is stable inside the fixing tube 52, providing stable support for subsequent puncture. During the sliding of the sliding rod 31 along the sleeve 51, the convex ring 35 will first conform to the sliding rod 31 and retract into the annular groove at the initial position. When the sliding rod 31 is pushed into place and the trapezoidal block 38 is engaged in the docking groove 57 to complete the locking, the convex ring 35 will spring into the corresponding groove 55, further improving the stability of the sliding rod 31. At this time, the viewing window 36 is aligned with the observation plate 54, and the test results of the test strip 44 can be read directly through the observation plate 54 and the viewing window 36. The entire process can be completed without opening the device, effectively avoiding the risk of contamination caused by sample leakage during the test, and also improving the convenience of the test operation.

[0041] The working principle of this invention is as follows: When in use, open the first sealing film 13, put the nucleic acid sample into the reaction tube 10, then put the receiving tube 21 into the reaction tube 10, then insert the receiving tube 21 into the receiving cavity 12, and then insert the reaction tube 10 into the fixing tube 52 so that the first buckle 15 is engaged in the first slot 53, thus completing the fixed connection between the reaction tube 10 and the fixing tube 52. Next, the test strip 44 is clamped and fixed inside the clamping plate 43. Then, the mounting block 41 is aligned with the insertion position on the upper left side of the sliding rod 31 and pushed in, so that the second buckle 45 is correspondingly engaged in the second slot 34, completing the pre-assembly with the sliding rod 31. Then, the sliding rod 31 is pushed along the sleeve 51 to move towards the receiving tube 21, so that the first puncture head 32 and the second puncture head 42 are aligned with the receiving tube 21 to puncture. At this time, the processing liquid in the receiving tube 21 mixes with the sample and reaction liquid in the reaction chamber 11. At the same time, the magnetic stirring rod 14 is driven to rotate by the external magnetic stirring device to mix the liquid and react. After the reaction is completed, the whole device is inverted, and the mixed liquid flows downward and flows into the channel 33 through the guide of the two sets of puncture heads to contact the test strip 44. Then, the test strip 44 reacts with the liquid to obtain the result, which can be observed through the observation plate 54 and the viewing window 36.

[0042] The above description is merely a preferred embodiment of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention. Structures, devices, and operating methods not specifically described or explained in this invention are implemented according to conventional methods in the art unless otherwise specified or limited.

Claims

1. A POCT card holder, characterized in that: include: The reaction tube has a first sealing membrane installed at its right end. The storage tube includes a receiving tube, which is placed inside the reaction tube. The detection tube includes a sliding rod, which is located on the right side of the reaction tube. A first puncture head is connected to the left side of the sliding rod. A channel is opened in the inner cavity of the sliding rod, and a viewing window is provided above the sliding rod. The fixing part includes a mounting block connected to the left end of the sliding rod. A second puncture head is connected to the left side of the mounting block, and a test strip is connected to the right side. The limiting tube includes a sleeve that is fitted around the outer ring of the sliding rod and the mounting block. A fixing tube is connected to the left end of the sleeve and is fitted around the outer ring of the reaction tube. The sliding rod slides along the sleeve, causing the first and second puncture heads to pierce the receiving tube, resulting in the liquid in the receiving tube mixing with the liquid in the reaction tube. Then the device is inverted to drive the mixed liquid into the channel to react with the test paper.

2. The POCT card holder according to claim 1, characterized in that: The reaction tube has a reaction chamber on the left side and a receiving chamber on the right side. The outer ring of the receiving tube is movably fitted into the receiving chamber, and the outer ring of the right end of the reaction tube is fixedly connected to a first sealing membrane.

3. The POCT card holder according to claim 2, characterized in that: A magnetic stirring rod is placed inside the reaction chamber, and the upper and lower sides of the right end of the outer ring of the reaction tube are fixedly connected with the first buckle.

4. The POCT card holder according to claim 2, characterized in that: The storage tube is placed inside the storage cavity, and a second sealing film is fixedly connected to both the front and rear ends of the storage tube.

5. The POCT card holder according to claim 2, characterized in that: The sliding rod is located at the right end of the reaction tube and the receiving tube. The first puncture head is fixedly connected to the lower side of the sliding rod facing the reaction tube. The inner cavity of the sliding rod has a channel with two sets of outlets, both of which are located at the left end of the sliding rod and are distributed vertically.

6. The POCT card holder according to claim 5, characterized in that: The upper and left ends of the sliding rod are provided with second slots. A convex ring is fixedly connected to the middle of the outer ring of the sliding rod. A through groove is provided between the two sets of convex rings on the outer ring of the sliding rod. The through groove is connected to the channel. A viewing window is fixedly connected inside the through groove.

7. The POCT card holder according to claim 6, characterized in that: A guide block is fixedly connected to the lower right end of the sliding rod. A cavity is opened at the upper right side of the outer ring of the sliding rod, and a trapezoidal block is slidably connected inside the cavity. A spring is fixedly connected to the lower end of the trapezoidal block, and the lower end of the spring is fixedly connected to the bottom of the cavity of the sliding rod.

8. The POCT card holder according to claim 6, characterized in that: The mounting block is movably inserted into the upper left side of the sliding rod. The left end of the mounting block is fixedly connected to a second puncture head, which is positioned above the first puncture head. The right end of the mounting block is fixedly connected to a clamping plate, which holds the test strip in the middle. Both the clamping plate and the test strip are movably inserted into the upper end of the channel cavity. The right side and lower end of the mounting block are fixedly connected to second buckles, which are engaged in second slots.

9. The POCT card holder according to claim 8, characterized in that: The sleeve is movably fitted onto the outer ring of the sliding rod and the mounting block. The left end of the sleeve is connected to a fixed tube, which is movably fitted onto the outer ring of the reaction tube. The right end of the inner cavity of the fixed tube has a first slot, and the first buckle is engaged in the inner cavity of the first slot. The upper end of the outer ring of the sleeve has a through groove, and an observation plate is fixedly connected in the through groove. The observation plate is positioned above the viewing window.

10. The POCT card holder according to claim 9, characterized in that: Two sets of grooves are provided in the middle of the inner cavity of the sleeve, and the convex ring is engaged in the groove. A guide groove is provided in the inner cavity of the right end of the sleeve, and the guide block is slidably connected in the guide groove. A docking groove is provided on the upper right side of the inner cavity of the sleeve, and the docking groove is located on the left side of the trapezoidal block.

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