A stem cell culture device

Abstract

This invention relates to the field of culture device technology, and more particularly to a stem cell culture device. The technical solution includes a culture chamber, within which a base plate is fixedly installed. A culture dish is slidably mounted on the base plate. The device also includes a first track frame, fixedly mounted on one side of the culture dish. A first motor is fixedly mounted on one side of the first track frame. A first reciprocating screw is rotatably mounted on the first track frame. The output shaft of the first motor is fixedly connected to the first reciprocating screw. A first controller and a first processor are disposed within the culture dish. A first slider is slidably mounted on the first track frame and threadedly connected to the first reciprocating screw. A second track frame is fixedly mounted on one side of the first slider. This invention achieves automatic medium replacement, precise layered liquid aspiration, zero stem cell loss, zero adsorption, low shear, and shock-free medium replacement, protecting cell membrane integrity. The liquid addition process automatically defoams, preventing bubble damage to cells.

Description

Technical Field

[0001] This invention relates to the field of culture device technology, and more particularly to a culture device for stem cells. Background Technology

[0002] Stem cells possess the potential for self-renewal, replication, and multi-lineage differentiation, making them invaluable for research and clinical applications in regenerative medicine, cell therapy, and drug screening. Large-scale, standardized in vitro culture is a crucial prerequisite for stem cell application. Regularly changing the culture medium, promptly removing metabolic wastes such as lactic acid and ammonia, and replenishing nutrients during the culture process are key steps in maintaining stem cell activity and ensuring their stemness and proliferative capacity.

[0003] Currently, traditional stem cell culture devices still have many shortcomings in the culture medium replacement process: First, most devices still rely on manual opening and changing of the medium. During operation, the sealed environment inside the incubator is disrupted, and key culture parameters such as temperature, CO2 concentration, and humidity fluctuate drastically, easily triggering stem cell stress responses, leading to apoptosis, loss of stemness, or premature differentiation. At the same time, external microorganisms can easily enter the culture system, causing contamination and seriously affecting the success rate and cell safety. Second, existing aspiration mechanisms are mostly set at a fixed height, unable to adaptively adjust the aspiration position according to changes in the liquid level. During aspiration, excessive local negative pressure can easily adsorb adherent stem cells or directly aspirate the bottom layer of cell suspension, causing a large loss of stem cells. Furthermore, excessive fluid shear force can directly damage the cell membrane, leading to a significant decrease in cell viability. Even with the addition of filtration structures in some devices, it is still difficult to avoid cell damage and loss caused by improper aspiration positions. Secondly, conventional liquid addition methods often employ direct dripping or point-to-point perfusion, where the liquid flow directly impacts the cell growth layer, generating strong fluid shear forces that can easily cause cell detachment and breakage of 3D cell aggregates. Simultaneously, air bubbles are easily generated during the liquid addition process. These bubbles, adhering to the cell surface, hinder nutrient and gas exchange, and the instantaneous impact force generated when bubbles burst can cause cell membrane perforation, further reducing cell viability. Furthermore, existing defoaming structures typically use independent drive motors for vibration defoaming, which is not only structurally complex and energy-intensive, but also difficult to precisely control the vibration amplitude. Excessive vibration can mechanically stimulate fragile stem cells, disrupting the cell growth microenvironment. Additionally, culture dishes are often rigidly fixed, lacking buffer structures, and the vibration generated by the device operation continuously interferes with cell growth. Moreover, the liquid aspiration and addition mechanisms are mostly point-to-point operations, failing to uniformly perform liquid replacement throughout the culture dish, easily leading to localized waste liquid residue and uneven nutrient replenishment, resulting in poor consistency in cell proliferation and differentiation states, and making it difficult to guarantee batch-to-batch stability. Finally, existing devices have a low level of automation and intelligence. The timing of fluid replacement and the control of fluid volume both rely on human experience and judgment, resulting in large operational errors. They cannot achieve closed-loop automatic control of the entire process and cannot meet the needs of standardized and large-scale clinical stem cell culture. Therefore, this application proposes a stem cell culture device. Summary of the Invention

[0004] The purpose of this invention is to address the problems of cell damage, loss, contamination, and unstable culture environment in the prior art, and to propose a stem cell culture device.

[0005] The technical solution of the present invention: A stem cell culture device includes a culture chamber, a base plate fixedly installed inside the culture chamber, a culture dish slidably installed on the base plate, and a first track frame fixedly installed on one side of the culture dish. A first motor is fixedly installed on one side of the first track frame, and a first reciprocating screw is rotatably installed on the first track frame. The output shaft of the first motor is fixedly connected to the first reciprocating screw. A first controller and a first processor are provided inside the culture dish. A first slider is slidably installed on the first track frame and threadedly connected to the first reciprocating screw. A second track frame is fixedly installed, a second reciprocating screw is rotatably installed on the second track frame, a second slider is slidably installed on the second track frame, a second connecting pipe is threadedly connected to the second reciprocating screw, a mounting plate is fixedly installed on one side of the second slider, a threaded rod is rotatably installed on the mounting plate, a positioning plate is slidably installed on the mounting plate, the positioning plate is threadedly connected to the threaded rod, a suction chamber is fixedly installed at the bottom of the positioning plate, the suction chamber is a hollow cavity, a first pump body is fixedly installed on one side of the incubator, a first connecting pipe is fixedly installed at one end of the first pump body, and one end of the first connecting pipe communicates with the suction chamber. Multiple suction heads are fixedly installed at the bottom of the suction chamber. A sterilizing filter is fixedly installed at the bottom of each suction head. A side plate is fixedly installed on one side of the suction chamber, and a detection block is fixedly installed on the side plate. A first float plate is slidably installed on the detection block, and a first pressure sensor is fixedly installed at one end of the first float plate. A second motor is fixedly installed on the mounting plate, and the output shaft of the second motor is fixedly connected to a threaded rod. A second controller and a second processor are disposed within the second motor. The output terminal of the first pressure sensor is connected to the input terminal of the second processor, and the output terminal of the second processor is connected to the input terminal of the second controller. The output of the second controller is connected to the start-up end of the second motor. A detection groove is provided inside the petri dish, and a second float plate is slidably installed inside the detection groove. A third pressure sensor is fixedly installed on the top of the second float plate, and a second pressure sensor is fixedly installed on the bottom of the second float plate. The output of the second pressure sensor is connected to the input of the first processor. The output of the first processor is connected to the input of the first controller. The output of the first controller is connected to the start-up ends of the first motor, the second motor, and the first pump body, respectively. The incubator is equipped with a conveying mechanism for uniformly adding liquid and removing air bubbles from the petri dish.

[0006] Optionally, the delivery mechanism includes a storage tank fixedly installed on one side of the incubator, a second pump body fixedly installed on one side of the storage tank, a second connecting pipe fixedly installed at one end of the second pump body, a third track frame fixedly installed on one side of the base plate, a third reciprocating screw rotatably installed inside the third track frame, a third slider slidably installed on the third track frame, the third slider being threadedly connected to the third reciprocating screw, a connecting cavity fixedly installed at the top of the third slider, the connecting cavity being hollow, one end of the second connecting pipe communicating with the connecting cavity, and infusion chambers fixedly installed on both sides of the bottom of the connecting cavity, the infusion chambers being hollow, and the bottom of the infusion chamber being fixedly installed... The culture dish is equipped with multiple infusion tubes. Infusion grooves are formed on both sides of the inner wall of the culture dish. One end of each infusion tube is located in the infusion groove. A guide rod is rotatably mounted on the bottom of the third slider. A first gear is rotatably mounted inside the incubator. A connecting rod is fixedly mounted on the bottom of the first gear. One end of the guide rod is rotatably connected to the connecting rod. A second gear is rotatably mounted on the base plate. The first gear meshes with the second gear. A third gear is rotatably mounted on the base plate. The second gear meshes with the third gear. Multiple first circular blocks arranged circumferentially at equal intervals are fixedly mounted on the third gear. Multiple second circular blocks arranged circumferentially at equal intervals are fixedly mounted on the bottom of the culture dish.

[0007] Optionally, a fourth motor is fixedly installed at one end of the second track frame, and the output shaft of the fourth motor is fixedly connected to the second reciprocating lead screw.

[0008] Optionally, a third motor is fixedly installed at one end of the third track frame, and the output shaft of the third motor is fixedly connected to the third reciprocating lead screw.

[0009] Optionally, the petri dish is equipped with a third controller and a third processor. The output terminal of the third pressure sensor is connected to the input terminal of the third processor, the output terminal of the third processor is connected to the input terminal of the third controller, and the output terminal of the third controller is connected to the start terminal of the third motor and the second pump body.

[0010] Optionally, a first spring is fixedly installed inside the detection block, and the first spring is sleeved on the first floating plate.

[0011] Optionally, a second spring is fixedly installed on both sides of the petri dish, and one end of the second spring is fixedly connected to the base plate.

[0012] Optionally, a sealed observation door is provided on one side of the incubator, and the sealed observation door is transparent.

[0013] Optionally, a collection tank is fixedly installed on one side of the incubator, and one side of the collection tank is fixedly connected to the first pump body.

[0014] Optionally, a support frame is fixedly installed on one side of the base plate, and the support frame is fixedly connected to the third track frame.

[0015] Compared with the prior art, the present invention has the following beneficial technical effects: This invention utilizes a liquid level detection system consisting of a floating first float and a pressure sensor, combined with adaptive height adjustment of the aspiration chamber, to precisely adhere to the upper liquid surface of the culture medium for aspiration. Only the upper waste liquid is aspirated, maintaining a safe distance between the aspiration head and the underlying stem cells. Combined with a two-dimensional moving mechanism consisting of a first reciprocating screw and a second reciprocating screw, the aspiration head achieves uniform reciprocating aspiration throughout the entire culture dish, fundamentally avoiding the strong negative pressure adsorption of stem cells caused by prolonged local aspiration, while also preventing damage from high shear forces during the aspiration process.

[0016] Furthermore, by employing a flow guide design in the inner wall of the culture dish at the liquid addition end, fresh culture medium flows in laminarly along the wall surface, rather than vertically scouring the cell layer; in conjunction with the third reciprocating screw driving the liquid addition chamber to reciprocate horizontally, uniform liquid distribution is achieved throughout the culture dish, avoiding fluid impact and shear force caused by continuous liquid addition at a single point, thus solving the problems of cell detachment and aggregate breakage caused by traditional liquid addition methods.

[0017] Furthermore, by utilizing the horizontal movement of the third slider, the rotation of the third gear is driven by the linkage of the guide rod, the first gear, and the second gear. This causes the first circular block to intermittently collide with the second circular block at the bottom of the culture dish, generating low-amplitude micro-vibrations to achieve physical defoaming. No additional vibration motor is required. The structure is compact and consumes no extra energy. With the elastic buffer of the second spring at the bottom of the culture dish, the micro-vibrations only act on the elimination of bubbles and do not transmit to the cell growth layer, avoiding vibration damage to stem cells. The system integrates a first floating plate pressure sensor, a second pressure sensor in the detection tank, and a third pressure sensor to automatically complete the adjustment of the liquid aspiration height, the determination of waste liquid aspiration, the quantitative addition of fresh culture medium, and the mechanism reset. The entire process requires no manual intervention or opening of the lid, realizing unmanned medium replacement of the entire stem cell culture process and eliminating human operation errors.

[0018] This invention achieves automatic liquid replacement, precise stratified liquid aspiration, zero loss and zero adsorption of stem cells, low shear and shock-free liquid replacement, protection of cell membrane integrity, automatic defoaming during liquid addition, and prevention of cell damage from air bubbles. Attached Figure Description

[0019] Figure 1 A schematic diagram of a stem cell culture device. Figure 1 ; Figure 2 A schematic diagram of a stem cell culture device. Figure 2 ; Figure 3 A schematic diagram of a stem cell culture device. Figure 3 ; Figure 4 This is a schematic diagram of the structure of the culture dish, connecting chamber, and infusion chamber; Figure 5 A schematic diagram of the structure of the first gear, the second gear, and the third gear; Figure 6 A schematic diagram of the detection block, suction head, and first float plate; Figure 7 This is a schematic diagram of the internal structure of the detection block; Figure 8 for Figure 3 A magnified schematic diagram of the partial structure at point A in the middle; Figure 9 for Figure 4 A magnified schematic diagram of the local structure at point B; Figure 10 for Figure 4 A magnified schematic diagram of the structure at point C in the middle; Figure 11 for Figure 5 A magnified schematic diagram of the structure at point D.

[0020] Reference numerals: 1. Incubator; 2. Sealed observation door; 3. Collection tank; 4. First pump body; 5. First connecting pipe; 6. Storage tank; 7. Second pump body; 8. Second connecting pipe; 9. Petri dish; 10. Base plate; 11. First motor; 12. First track frame; 13. First slider; 14. First reciprocating screw; 15. Second track frame; 16. Infusion tank; 17. Infusion tube; 18. Second slider; 19. Second reciprocating screw; 20. Mounting plate; 21. Threaded rod; 22. Second motor; 23. Positioning plate; 24. Suction chamber; 25. Detection block; 26. Side plate; 27. Suction head; 28. First float plate; 29. ​​Sterilizing filter; 30. First pressure sensor; 31. First spring; 32. Detection tank; 33. Second float plate; 34. Second pressure sensor; 35. Third pressure sensor; 36. Third track frame; 37. Third motor; 38. Fourth motor; 39. Third slider; 40. Guide rod; 41. First gear; 42. Connecting rod; 43. Second gear; 44. Third gear; 45. First circular block; 46. Second circular block; 47. Second spring; 48. Connecting cavity; 49. Infusion cavity; 50. Third reciprocating screw. Detailed Implementation

[0021] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0022] The components of the embodiments of the invention described and shown in the accompanying drawings can typically be arranged and designed in a variety of different configurations. Therefore, the following detailed description of the embodiments of the invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention.

[0023] Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0024] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0025] It should be noted that the terms "comprising," "including," or any other variations thereof are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0026] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0027] Example 1 like Figure 1-10As shown, the present invention proposes a stem cell culture device, including a culture chamber 1, a base plate 10 fixedly installed inside the culture chamber 1, and a culture dish 9 slidably installed on the base plate 10 for culturing stem cells. It also includes a first track frame 12, which is fixedly installed on one side of the culture dish 9. A first motor 11 is fixedly installed on one side of the first track frame 12, and a first reciprocating screw 14 is rotatably installed on the first track frame 12. The output shaft of the first motor 11 is fixedly connected to the first reciprocating screw 14. A first controller and a first processor are provided inside the culture dish 9. A first slider 13 is slidably mounted on a track frame 12. The first slider 13 is threadedly connected to a first reciprocating screw 14. A second track frame 15 is fixedly mounted on one side of the first slider 13. A second reciprocating screw 19 is rotatably mounted on the second track frame 15. A second slider 18 is slidably mounted on the second track frame 15. A second connecting pipe 8 is threadedly connected to the second reciprocating screw 19. A mounting plate 20 is fixedly mounted on one side of the second slider 18. A threaded rod 21 is rotatably mounted on the mounting plate 20. A positioning plate 23 is slidably mounted on the mounting plate 20. The positioning plate 23 and the threaded rod 21 are connected. 1. A threaded connection is used. A suction chamber 24 is fixedly installed at the bottom of the positioning plate 23. The suction chamber 24 is a hollow cavity. A first pump body 4 is fixedly installed on one side of the incubator 1. A first connecting pipe 5 is fixedly installed at one end of the first pump body 4, and one end of the first connecting pipe 5 communicates with the suction chamber 24. Multiple suction heads 27 are fixedly installed at the bottom of the suction chamber 24. A sterilization filter 29 is fixedly installed at the bottom of the suction head 27 to filter stem cells and prevent them from being aspirated. A side plate 26 is fixedly installed on one side of the suction chamber 24, and a detection block 25 is fixedly installed on the side plate 26 for detection. A first float plate 28 is slidably mounted on block 25. A first pressure sensor 30 is fixedly mounted on one end of the first float plate 28. A second motor 22 is fixedly mounted on mounting plate 20. The output shaft of the second motor 22 is fixedly connected to the threaded rod 21. A second controller and a second processor are provided inside the second motor 22. The output end of the first pressure sensor 30 is connected to the input end of the second processor. The output end of the second processor is connected to the input end of the second controller. The output end of the second controller is connected to the start end of the second motor 22. A detection groove 32 is opened inside the petri dish 9.A second float plate 33 is slidably installed inside the detection tank 32 for detecting the depth of the culture medium. A third pressure sensor 35 is fixedly installed on the top of the second float plate 33, and a second pressure sensor 34 is fixedly installed on the bottom of the second float plate 33. The output end of the second pressure sensor 34 is connected to the input end of the first processor, and the output end of the first processor is connected to the input end of the first controller. The output end of the first controller is connected to the start end of the first motor 11, the second motor 22, and the first pump body 4, respectively. When the culture medium needs to be replaced, the first motor 11 is started. The output shaft of the first motor 11 drives the first reciprocating screw 14 to rotate. The first reciprocating screw 14 drives the first slider 13 to perform horizontal reciprocating motion on the first track frame 12. The second motor 22 is activated, and its output shaft drives the threaded rod 21 to rotate, which in turn moves the positioning plate 23 downward. The positioning plate 23 moves the bottom suction chamber 24 downward, and the suction head 27 enters the upper layer of the culture medium. At the same time, the first float plate 28 contacts the upper liquid surface. As the suction chamber 24 descends, the first float plate 28 drives the first pressure sensor 30 to contact the top of the detection block 25. At this time, the first pressure sensor 30 transmits a signal to the second processor. The second processor determines that the suction chamber 24 is in contact with the upper liquid and transmits a signal to the second controller, activating the first pump body 4. The first pump body 4 draws the upper liquid away from the suction head 27. As the upper liquid continues to decrease... The first float 28 moves downwards, the first pressure sensor 30 separates from the detection block 25, and the second processor determines that the aspiration chamber 24 is separated from the supernatant and cannot complete the aspiration task. It then transmits a signal to the second controller, which starts the second motor 22 to move the aspiration chamber 24 downwards until the first pressure sensor 30 contacts the top of the detection block 25. This, in conjunction with the rotation of the second reciprocating screw 19, causes the aspiration chamber 24 to reciprocate horizontally on the second track frame 15, thoroughly and evenly aspirating the supernatant in the culture dish 9. This prevents the aspiration chamber 24 from remaining in the culture dish 9 for too long and thus aspirating the underlying stem cells. As the supernatant decreases, the second float 33 moves downwards. When the second pressure sensor 34 contacts the bottom of the detection tank 32, it transmits a signal to the first processor. The first processor determines that the upper layer liquid removal is complete and transmits a signal to the first controller. The first controller stops the first pump body 4 and drives the first motor 11 and the second motor 22 to reverse, resetting the suction chamber 24, the first slider 13, and the second slider 18. At this point, the suction program is complete, accurately removing the upper waste liquid while retaining the culture medium containing cell autocrine factors at the bottom layer. This avoids sudden changes in the microenvironment caused by a complete medium change, reduces stem cell stress, and lowers the probability of premature differentiation and apoptosis. The controller's incubator 1 is equipped with a delivery mechanism that uniformly adds liquid to the culture dish 9 and removes air bubbles.

[0028] Example 2 like Figure 4 , Figure 5 and Figure 11As shown, the conveying mechanism includes a liquid storage tank 6 fixedly installed on one side of the incubator 1, a second pump body 7 fixedly installed on one side of the liquid storage tank 6, a second connecting pipe 8 fixedly installed at one end of the second pump body 7, a third track frame 36 fixedly installed on one side of the base plate 10, a third reciprocating screw 50 rotatably installed inside the third track frame 36, a third slider 39 slidably installed on the third track frame 36, the third slider 39 being threadedly connected to the third reciprocating screw 50, and a connecting cavity 48 fixedly installed on the top of the third slider 39, the connecting cavity 48 being a hollow cavity.One end of the second connecting pipe 8 is connected to the connecting cavity 48. Infusion cavities 49 are fixedly installed on both sides of the bottom of the connecting cavity 48. The infusion cavities 49 are hollow. Multiple infusion tubes 17 are fixedly installed at the bottom of the infusion cavities 49. Infusion grooves 16 are opened on both sides of the inner wall of the culture dish 9. One end of each infusion tube 17 is located inside the infusion groove 16. A guide rod 40 is rotatably installed at the bottom of the third slider 39. A first gear 41 is rotatably installed inside the incubator 1. A connecting rod 42 is fixedly installed at the bottom of the first gear 41. One end of the guide rod 40 is rotatably connected to the connecting rod 42. A second gear 43 is rotatably installed on the base plate 10, meshing with the first gear 41. A third gear 44 is rotatably installed on the base plate 10, meshing with the second gear 43. The third gear 44 is fixedly mounted with multiple first circular blocks 45 arranged at equal intervals in a circular pattern. The bottom of the culture dish 9 is fixedly mounted with multiple second circular blocks 46 arranged at equal intervals in a circular pattern. The culture dish 9 is equipped with a third controller and a third processor. The output end of the third pressure sensor 35 is connected to the input end of the third processor, the output end of the third processor is connected to the input end of the third controller, and the output end of the third controller is connected to the start end of the third motor 37 and the second pump body 7. After the liquid aspiration program is completed, the second pump body 7 is started. The second pump body 7 draws the culture medium in the storage tank 6 into the connecting cavity 48, and finally delivers it from the connecting cavity 48 to the infusion tube 17, and then from the infusion tube 17 to the infusion tank 16, flowing downwards along the inner wall of the culture dish 9. To minimize air bubbles generated by directly adding culture medium, the third reciprocating screw 50 is rotated. This screw drives the third slider 39 to reciprocate horizontally on the third track frame 36. The slider 39, in turn, drives the connecting cavity 48 to reciprocate horizontally, ensuring the infusion tube 17 evenly adds culture medium to the culture dish 9. This avoids prolonged impact on a single location, preventing stem cell damage. Simultaneously, the third slider moves a guide rod 40 on one side, which compresses or stretches the connecting rod 42. This causes the connecting rod 42 to rotate the first gear 41, which in turn rotates the second gear 43. The second gear 43 then rotates the third gear 44. The first circular block 45 on the third gear 44 continuously contacts the second circular block 46, thereby... The vibration of the culture dish 9 further removes air bubbles generated during the culture medium addition process, preventing residual bubbles from rupturing and causing cell membrane perforation. As culture medium is continuously added, the second float plate 33 moves upward, causing the third pressure sensor 35 to contact the detection tank 32 and transmit a signal to the third processor. The third processor determines that there is enough culture medium and transmits a signal to the third controller. The third controller stops the second pump body 7 and starts the fourth motor 38, moving the third slider 39 to one side to complete the reset. At this point, the culture medium addition process is complete, minimizing damage to stem cells during culture medium replacement and avoiding the instantaneous impact damage caused by air bubble adhesion, which can obstruct cell nutrient exchange, or by air bubble rupture.

[0029] Example 3 like Figure 7-11 As shown, a fourth motor 38 is fixedly installed at one end of the second track frame 15. The output shaft of the fourth motor 38 is fixedly connected to the second reciprocating lead screw 19. Starting the fourth motor 38 drives the second reciprocating lead screw 19 to rotate. A third motor 37 is fixedly installed at one end of the third track frame 36. The output shaft of the third motor 37 is fixedly connected to the third reciprocating lead screw 50. Starting the third motor 37 drives the third reciprocating lead screw 50 to rotate. A first spring 31 is fixedly installed inside the detection block 25. The first spring 31 is sleeved on the first float plate 28. A second spring 47 is fixedly installed on both sides of the culture dish 9. One end of the second spring 47 is fixedly connected to the bottom plate 10. A sealed observation door 2 is provided on one side of the incubator 1. The sealed observation door 2 is transparent, which facilitates the observation of cell growth in the culture dish 9. A collection tank 3 is fixedly installed on one side of the incubator 1. One side of the collection tank 3 is fixedly connected to the first pump body 4. A support frame is fixedly installed on one side of the bottom plate 10. The support frame is fixedly connected to the third track frame 36.

[0030] Working principle: When the culture medium needs to be replaced, the first motor 11 is started. The output shaft of the first motor 11 drives the first reciprocating screw 14 to rotate. The first reciprocating screw 14 drives the first slider 13 to perform horizontal reciprocating motion on the first track frame 12. The second motor 22 is started. The output shaft of the second motor 22 drives the threaded rod 21 to rotate, which in turn drives the positioning plate 23 to move downward. The positioning plate 23 drives the bottom suction chamber 24 to move downward, and the suction head 27 enters the upper layer of the culture medium. At the same time, the first float plate 28 contacts the upper liquid surface. As the suction chamber 24 descends, the first float plate 28 drives the first pressure sensor 30 to contact the top of the detection block 25. At this time, the first pressure sensor 30 transmits a signal to the second processor. The second processor determines the current position of the suction chamber. When the aspiration chamber 24 comes into contact with the upper layer of liquid, the second processor transmits a signal to the second controller, activating the first pump 4. The first pump 4 draws the upper layer of liquid away from the aspiration head 27. As the upper layer of liquid decreases, the first float 28 moves downward, and the first pressure sensor 30 separates from the detection block 25. The second processor determines that the aspiration chamber 24 is separated from the upper layer of liquid and cannot complete the aspiration task. It then transmits a signal to the second controller, which activates the second motor 22 to move the aspiration chamber 24 downward until the first pressure sensor 30 contacts the top of the detection block 25. This, in conjunction with the rotation of the second reciprocating screw 19, causes the aspiration chamber 24 to reciprocate horizontally on the second track frame 15, ensuring thorough and uniform aspiration of the upper layer of liquid in the culture dish 9. In addition, to prevent the aspiration chamber 24 from remaining in the culture dish 9 for too long and thus aspirating the stem cells at the bottom layer, as the upper layer liquid decreases, the second float 33 moves downward. When the second pressure sensor 34 contacts the bottom of the detection tank 32, the second pressure sensor 34 transmits a signal to the first processor. The first processor determines that the upper layer liquid aspiration is complete and transmits a signal to the first controller. The first controller controls the first pump body 4 to stop and drives the first motor 11 and the second motor 22 to reverse, causing the aspiration chamber 24, the first slider 13, and the second slider 18 to reset. At this point, the aspiration process is complete, accurately aspirating the upper waste liquid while retaining the culture medium containing cell autocrine factors at the bottom layer. This avoids sudden changes in the microenvironment caused by a complete medium change, reduces stem cell stress response, and lowers the risk of premature differentiation. The apoptosis probability is determined by activating the second pump 7, which draws the culture medium from the storage tank 6 into the connecting chamber 48. The medium is then transported from the connecting chamber 48 to the infusion tube 17, and from the infusion tube 17 to the infusion tank 16, flowing downwards along the inner wall of the culture dish 9. This reduces air bubbles generated by directly adding culture medium. Simultaneously, the third reciprocating screw 50 rotates, causing the third slider 39 to reciprocate horizontally on the third track frame 36. The third slider 39 also causes the connecting chamber 48 to reciprocate horizontally, ensuring that the infusion tube 17 evenly adds culture medium to the culture dish 9, avoiding prolonged impact on a single location that could damage stem cells. At the same time, the third slider moves the guide rod 40 on one side, which compresses or stretches the connecting rod 42.The connecting rod 42 drives the first gear 41 to rotate, which in turn drives the second gear 43 to rotate. The second gear 43 drives the third gear 44 to rotate. The first circular block 45 on the third gear 44 continuously contacts the second circular block 46, causing the culture dish 9 to vibrate. This further removes air bubbles generated during the liquid addition process, preventing residual bubbles from rupturing and causing cell membrane perforation. As the culture medium is continuously added, the second float plate 33 moves upward, causing the third pressure sensor 35 to contact the detection tank 32 and transmit a signal to the third processor. The third processor determines that there is enough culture medium and transmits a signal to the third controller. The third controller stops the second pump body 7 and starts the fourth motor 38, moving the third slider 39 to one side to complete the reset. At this point, the liquid addition program is complete, achieving automatic liquid replacement, precise stratified liquid aspiration, zero stem cell loss, zero adsorption, low shear, and impact-free liquid replacement, protecting cell membrane integrity. The liquid addition process automatically defoams, preventing air bubbles from damaging cells.

[0031] The above specific embodiments are merely several optional embodiments of the present invention. Based on the technical solutions of the present invention and the relevant teachings of the above embodiments, those skilled in the art can make various alternative improvements and combinations to the above specific embodiments.

Claims

1. A stem cell culture device, comprising a culture chamber (1), wherein a base plate (10) is fixedly installed inside the culture chamber (1), and a culture dish (9) is slidably installed on the base plate (10), characterized in that, It also includes a first track frame (12), which is fixedly installed on one side of the petri dish (9). A first motor (11) is fixedly installed on one side of the first track frame (12). A first reciprocating screw (14) is rotatably installed on the first track frame (12). The output shaft of the first motor (11) is fixedly connected to the first reciprocating screw (14). A first controller and a first processor are provided inside the petri dish (9). A first slider (13) is slidably installed on the first track frame (12). The first slider (13) is threadedly connected to the first reciprocating screw (14). A second track frame (15) is fixedly installed on one side of the first slider (13). A second track frame (15) is rotatably installed on the second track frame (15). The second reciprocating screw (19) is slidably mounted on the second track frame (15), the second connecting pipe (8) is threadedly connected to the second reciprocating screw (19), the second slider (18) is fixedly mounted on one side of the second slider (18), the threaded rod (21) is rotatably mounted on the mounting plate (20), the positioning plate (23) is slidably mounted on the mounting plate (20), the positioning plate (23) is threadedly connected to the threaded rod (21), the bottom of the positioning plate (23) is fixedly mounted with a suction chamber (24), the suction chamber (24) is a cavity, the first pump body (4) is fixedly mounted on one side of the incubator (1), and the first connecting pipe (5) is fixedly mounted on one end of the first pump body (4). One end of the first connecting pipe (5) is connected to the suction chamber (24). Multiple suction heads (27) are fixedly installed at the bottom of the suction chamber (24). A sterilization filter (29) is fixedly installed at the bottom of the suction head (27). A side plate (26) is fixedly installed on one side of the suction chamber (24). A detection block (25) is fixedly installed on the side plate (26). A first float plate (28) is slidably installed on the detection block (25). A first pressure sensor (30) is fixedly installed at one end of the first float plate (28). A second motor (22) is fixedly installed on the mounting plate (20). The output shaft of the second motor (22) is fixedly connected to the threaded rod (21). A second control is provided inside the second motor (22). The system comprises a device and a second processor. The output of the first pressure sensor (30) is connected to the input of the second processor. The output of the second processor is connected to the input of the second controller. The output of the second controller is connected to the start-up terminal of the second motor (22). A detection groove (32) is provided inside the petri dish (9). A second float plate (33) is slidably installed inside the detection groove (32). A third pressure sensor (35) is fixedly installed on the top of the second float plate (33). A second pressure sensor (34) is fixedly installed on the bottom of the second float plate (33). The output of the second pressure sensor (34) is connected to the input of the first processor. The output of the first processor is connected to the input of the first controller.The output of the first controller is connected to the starting terminals of the first motor (11), the second motor (22), and the first pump body (4), respectively. The incubator (1) is equipped with a conveying mechanism for uniformly adding liquid and removing air bubbles from the culture dish (9).

2. The stem cell culture device according to claim 1, characterized in that, The delivery mechanism includes a storage tank (6) fixedly installed on one side of the incubator (1), a second pump body (7) fixedly installed on one side of the storage tank (6), a second connecting pipe (8) fixedly installed at one end of the second pump body (7), a third track frame (36) fixedly installed on one side of the base plate (10), a third reciprocating screw (50) rotatably installed inside the third track frame (36), a third slider (39) slidably installed on the third track frame (36), the third slider (39) being threadedly connected to the third reciprocating screw (50), a connecting cavity (48) fixedly installed on the top of the third slider (39), the connecting cavity (48) being hollow, one end of the second connecting pipe (8) communicating with the connecting cavity (48), infusion chambers (49) fixedly installed on both sides of the bottom of the connecting cavity (48), the infusion chambers (49) being hollow, and multiple infusion chambers (49) fixedly installed at the bottom of the infusion chambers (49). The inner wall of the petri dish (9) is provided with infusion tanks (16) on both sides. One end of the infusion tube (17) is located in the infusion tank (16). The bottom of the third slider (39) is rotatably mounted with a guide rod (40). The first gear (41) is rotatably mounted in the incubator (1). The bottom of the first gear (41) is fixedly mounted with a connecting rod (42). One end of the guide rod (40) is rotatably connected to the connecting rod (42). The second gear (43) is rotatably mounted on the base plate (10). The first gear (41) meshes with the second gear (43). The third gear (44) is rotatably mounted on the base plate (10). The second gear (43) meshes with the third gear (44). Multiple first circular blocks (45) are fixedly mounted on the third gear (44) at equal intervals. Multiple second circular blocks (46) are fixedly mounted on the bottom of the petri dish (9).

3. The stem cell culture device according to claim 1, characterized in that, A fourth motor (38) is fixedly installed at one end of the second track frame (15), and the output shaft of the fourth motor (38) is fixedly connected to the second reciprocating lead screw (19).

4. A stem cell culture device according to claim 2, characterized in that, A third motor (37) is fixedly installed at one end of the third track frame (36), and the output shaft of the third motor (37) is fixedly connected to the third reciprocating lead screw (50).

5. A stem cell culture device according to claim 2, characterized in that, The petri dish (9) is equipped with a third controller and a third processor. The output end of the third pressure sensor (35) is connected to the input end of the third processor. The output end of the third processor is connected to the input end of the third controller. The output end of the third controller is connected to the start end of the third motor (37) and the second pump body (7).

6. A stem cell culture device according to claim 1, characterized in that, A first spring (31) is fixedly installed inside the detection block (25), and the first spring (31) is sleeved on the first float plate (28).

7. A stem cell culture device according to claim 2, characterized in that, The culture dish (9) is fixedly installed with a second spring (47) on both sides, and one end of the second spring (47) is fixedly connected to the base plate (10).

8. A stem cell culture device according to claim 1, characterized in that, A sealed observation door (2) is provided on one side of the incubator (1), and the sealed observation door (2) is transparent.

9. A stem cell culture device according to claim 1, characterized in that, A collection tank (3) is fixedly installed on one side of the incubator (1), and one side of the collection tank (3) is fixedly connected to the first pump body (4).

10. A stem cell culture device according to claim 2, characterized in that, A support frame is fixedly installed on one side of the base plate (10), and the support frame is fixedly connected to the third track frame (36).

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