A method for promoting high-temperature germination of rattlepod seeds
Patent Information
- Application Number
- CN202611062767.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-07-17
- Publication Date
- 2026-08-18
AI Technical Summary
[0003]目前黄花猪屎豆在自然条件下的萌发率较低在30%左右，通过机械摩擦或者70℃热水浸泡能够有效提升种子的萌发率，但是这些萌发率都是在较适宜的环境中萌发，一般温度都是控制在15-20℃，对于荒漠地区或者土壤贫瘠的生态环境脆弱地区来说往往会出现高温或者干旱的环境，干旱时可以通过人工灌溉来处理，但是高温往往无法直接改善，而黄花猪屎豆种子在30℃以上发芽率会显著受到抑制，甚至不发芽，基于此，严重限制了黄花猪屎豆在高温地区对于土壤改良的应用，所以如何提升黄花猪屎豆种子高温环境中的发芽并且保证后续的种苗生长是现阶段一大重要的研究方向
本发明通过对黄花猪屎豆种子进行低温高压处理（0-5℃，0.2-0.3Mpa）后高温常压处理（40-50℃），并控制两次处理的温差（≤40℃），联合后续的淀粉赤霉素溶液超声处理种子，有效促进种子在高温环境中的萌发，同时提升后续萌发后幼苗的生长效果和抗逆效果，为高温干旱地区提供良好的土壤改良植被，能够综合提升后续的改良效果。
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Abstract
Description
Technical Field
[0001] This invention relates to the field of seed germination promotion technology for *Pterocarya stenoptera*, specifically to a method for promoting high-temperature germination of *Pterocarya stenoptera* seeds. Background Technology
[0002] Yellow-flowered pig manure bean is an annual or perennial herb with a well-developed root system that can effectively fix nitrogen and improve soil structure. It is a good desertification improvement plant, and large-scale planting can effectively improve soil desertification.
[0003] Currently, the germination rate of *Gnaphalium affine* under natural conditions is relatively low, around 30%. Mechanical friction or soaking in 70℃ hot water can effectively improve the germination rate. However, these germination rates are achieved in relatively suitable environments, generally with temperatures controlled between 15-20℃. In arid regions or ecologically fragile areas with poor soil, high temperatures or drought are common. Drought can be addressed through artificial irrigation, but high temperatures are often difficult to improve directly. Furthermore, the germination rate of *Gnaphalium affine* seeds is significantly inhibited or even fails to germinate above 30℃. This severely limits the application of *Gnaphalium affine* in soil improvement in high-temperature regions. Therefore, improving the germination rate of *Gnaphalium affine* seeds in high-temperature environments and ensuring subsequent seedling growth is a major research direction at present. Summary of the Invention
[0004] To address the shortcomings of existing technologies, this invention provides a method for promoting the high-temperature germination of *Phyllostachys edulis* seeds, effectively promoting germination in high-temperature environments and enhancing subsequent growth, thereby comprehensively improving the application prospects of *Phyllostachys edulis* for soil improvement in hot and arid regions.
[0005] To achieve the above objectives, the present invention provides the following technical solution: A method for promoting high-temperature germination of *Pteris vittata* seeds, the method comprising the following steps: S1. Select plump, insect-free, undamaged, and well-developed yellow-flowered pig manure bean seeds and disinfect them to obtain disinfected seeds for later use.
[0006] S2. The disinfected seeds are placed in a water bath at 0-5℃ and pressurized to 0.2-0.3 MPa for 10-20 minutes. Then, the pressure is restored to normal and the seeds are placed in warm water at 40-45℃ for 5-10 minutes to obtain pretreated seeds for later use.
[0007] S3. Add the pretreated seeds to a starch solution and sonicate them, then wash them clean, soak them in water and set aside.
[0008] S4. Germinate the soaked seeds at high temperature.
[0009] Preferably, in step S1, the seeds of the yellow-flowered pig manure bean are disinfected by soaking in a 0.1% potassium permanganate solution for 5 minutes, followed by repeated rinsing with clean water.
[0010] Preferably, in step S2, the temperature difference between the two treatments at 0-5℃ and 40-45℃ is ≤40℃.
[0011] Preferably, the mass concentration of the starch solution in step S3 is 20%-30%.
[0012] Preferably, the starch solution also contains 200 mmol / L of gibberellin.
[0013] Preferably, in step S3, the ultrasonic treatment is performed at a power of 400-600W for 3-5 minutes, followed by resting for 5-8 minutes, and then continuing ultrasonic treatment for another 3-5 minutes. This process is repeated until the total ultrasonic time is 15-25 minutes.
[0014] Preferably, the soaking time in clean water in step S3 is 30-40 minutes.
[0015] Preferably, the temperature for high-temperature germination in step S4 is 30-38℃, and the ambient humidity during germination is 80%-90%.
[0016] Preferably, the light conditions for high-temperature germination in step S4 are a 12-hour light / 12-hour dark cycle, and the light intensity is 2000 lx.
[0017] This invention provides a method for promoting the high-temperature germination of *Pteris vittata* seeds, which has the following advantages compared to existing technologies: This invention involves subjecting yellow-flowered pig manure bean seeds to low-temperature high-pressure treatment (0-5℃, 0.2-0.3 MPa) followed by high-temperature normal-pressure treatment (40-50℃), with the temperature difference between the two treatments controlled (≤40℃). Combined with subsequent ultrasonic treatment of the seeds with starch gibberellin solution, this effectively promotes seed germination in a high-temperature environment and enhances the growth and stress resistance of seedlings after germination. This provides a good soil-improving vegetation for hot and arid regions and comprehensively improves the subsequent improvement effect. Attached Figure Description
[0018] Figure 1 This is a schematic diagram of the seed germination of *Pterocarya stenoptera* in the control group of this invention.
[0019] Figure 2 This is a schematic diagram of the seed germination of *Pteris vittata* in experimental group 1 of this invention.
[0020] Figure 3 This is a schematic diagram of the seed germination of *Pteris vittata* in experimental group 7 of this invention.
[0021] Figure 4 This is a schematic diagram of the germination of seeds of the yellow-flowered pig manure bean in experimental group 8 in an embodiment of the present invention. Detailed Implementation
[0022] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0023] The seeds of the yellow-flowered pig manure bean used below were collected from the rocky desertification ecological protection area in Yunnan Province in the autumn of 2024. The seeds underwent strict selection to ensure that they were plump, free from pests and diseases, free from mechanical damage, and well-developed, and were stored at a constant temperature of 4℃ to maintain their viability.
[0024] Before use, the seeds were disinfected by soaking in a 0.1% potassium permanganate solution for 5 minutes, and then rinsed repeatedly with clean water 5 times to obtain disinfected seeds.
[0025] Example: I. Seed treatment method: 1. Preparation of Seed Treatment 1: (1) The disinfected seeds were treated in a water bath at 3℃, pressurized to 0.2 MPa for 15 min, then restored to normal pressure and placed in warm water at 42℃ for 8 min to obtain pretreated seeds for use.
[0026] (2) Add the pretreated seeds to a solution with a starch mass concentration of 25% and a gibberellin concentration of 200 mmol / L, treat for 5 min at 600 W, let stand for 5 min, then continue to sonicate for 5 min, repeat this operation until the total sonication time is 20 min, then clean and soak in water for 35 min to obtain treated seed 1.
[0027] 2. Preparation of Seed Treatment 2: (1) The disinfected seeds were treated at 3℃ water bath temperature, pressurized to 0.2Mpa for 15min, then restored to normal pressure and placed in 45℃ warm water for 8min to obtain pretreated seeds for use.
[0028] (2) Add the pretreated seeds to a solution with a starch mass concentration of 25% and a gibberellin concentration of 200 mmol / L, treat for 5 min at 600 W, let stand for 5 min, and then continue to sonicate for 5 min. Repeat this operation until the total sonication time is 20 min. Then clean the seeds and soak them in water for 35 min to obtain treated seeds 2.
[0029] 3. Preparation of Seed Treatment 3: (1) The disinfected seeds were treated in a water bath at 3°C for 15 minutes, and then placed in warm water at 42°C for 8 minutes to obtain pretreated seeds for use.
[0030] (2) Add the pretreated seeds to a solution with a starch mass concentration of 25% and a gibberellin concentration of 200 mmol / L, treat for 5 min at 600 W, let stand for 5 min, then continue to sonicate for 5 min, repeat this operation until the total sonication time is 20 min, then clean and soak in water for 35 min to obtain treated seeds 3.
[0031] 4. Preparation of Seed Treatment 4: Disinfected seeds were added to a solution with a starch concentration of 25% and a gibberellin concentration of 200 mmol / L, treated at 600W for 5 minutes, then allowed to stand for 5 minutes, and then sonicated for another 5 minutes. This process was repeated until the total sonication time was 20 minutes. After cleaning, the seeds were soaked in water for 35 minutes to obtain treated seed 4.
[0032] 5. Preparation of Seed Treatment 5: (1) The disinfected seeds were treated in a water bath at 3℃, pressurized to 0.2 MPa for 15 min, then restored to normal pressure and placed in warm water at 42℃ for 8 min to obtain pretreated seeds for use.
[0033] (2) Add the pretreated seeds to a gibberellin solution with a concentration of 200 mmol / L, treat for 5 min at 600 W, let stand for 5 min, then continue to sonicate for 5 min, repeat this operation until the total sonication time is 20 min, then clean and soak in water for 35 min to obtain treated seeds 5.
[0034] 6. Preparation of Seed Treatment 6: (1) The disinfected seeds were treated in a water bath at 3℃, pressurized to 0.2 MPa for 15 min, then restored to normal pressure and placed in warm water at 42℃ for 8 min to obtain pretreated seeds for use.
[0035] (2) The pretreated seeds were added to a solution with a starch mass concentration of 25% and a gibberellin concentration of 200 mmol / L, and treated for 20 min at a power of 600 W. After cleaning, the seeds were soaked in water for 35 min to obtain treated seeds 6.
[0036] 7. Preparation of Seed Treatment 7 (Mechanical Treatment): The disinfected seeds were rubbed between two sheets of sandpaper for 5 minutes to obtain treated seeds 7.
[0037] 8. Preparation of Seed Treatment 8 (High-Temperature Soaking Treatment): The disinfected seeds were soaked in 70℃ warm water for 5 minutes to obtain treated seeds 8.
[0038] II. Seed high-temperature germination experiment: The experiment was conducted in the laboratory of Yunnan Forestry Vocational and Technical College from June 23 to July 14, 2025. The experimental environment was controlled at a temperature of 37℃±0.5℃, a relative humidity of 86%±2%, and a 12-hour light / 12-hour dark cycle (light intensity approximately 2000 lx). Seeds were treated on June 23, placed in seedbeds on June 24, and germination was recorded from June 25. Each treatment had three replicates, with 50 seeds per replicate. The specific method was as follows: Seeds from each group were placed in 9 cm diameter petri dishes lined with double layers of Whatman No. 1 filter paper and 5 mL of distilled water was added. The petri dishes were placed in a constant temperature incubator, and the filter paper moisture was checked daily, with approximately 1-2 mL of distilled water added to maintain humidity. Seed germination was recorded daily at a fixed time (8:00 AM) for 21 consecutive days. Germination was defined as the radicle breaking through the seed coat and reaching a length of 2 mm or more.
[0039] 1. Record the germination parameters of each group of seeds: (1) Germination rate (%) = (number of germinated seeds / number of tested seeds) × 100%, which reflects the overall germination ability of seeds.
[0040] (2) Relative germination rate (%) = (germination rate of treatment group / germination rate of control group) × 100%, used to compare the germination difference between the treatment group and the control group.
[0041] The specific test results are shown in Table 1 below: Table 1
[0042] 2. Detection of physiological and biochemical indicators: At the end of day 21 of the experiment, seedlings (including aboveground parts and roots) were collected from the control group, experimental group 1, experimental group 3, experimental group 6 (groups with relatively high germination rates), and experimental group 7 and experimental group 8, and the following physiological and biochemical indicators were measured: (1) Malondialdehyde (MDA): The absorbance was measured at wavelengths of 532 nm and 600 nm using the thiobarbituric acid method. The content of membrane lipid peroxidation products was calculated, and the unit was nmol / g fresh weight.
[0043] (2) Soluble sugars: The absorbance was measured at a wavelength of 620 nm using the anthrone colorimetric method to reflect the carbohydrate metabolism level of seedlings. The unit was mg / g fresh weight.
[0044] (3) Proline: The absorbance was measured at a wavelength of 520 nm using the acidic ninhydrin method to reflect the osmotic regulation capacity of seedlings. The unit was μg / g fresh weight.
[0045] (4) Antioxidant enzyme activity: Using kits provided by Nanjing Jiancheng Bioengineering Research Institute, the activities of peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD) were measured to reflect the seedlings' defense against oxidative stress. The units were U / g fresh weight. The measurement process was strictly carried out in accordance with the kit instructions. The samples were homogenized at 4℃ and analyzed immediately.
[0046] The specific results are shown in Table 2 below: Table 2
[0047] Based on the above tests, experimental group 1 showed good technical results, effectively promoting the germination of *Phyllanthus urinaria* seeds in a high-temperature environment and enhancing the seedlings' resistance to adverse conditions, providing a good method for soil improvement in arid and hot regions.
[0048] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims
1. A method for promoting high-temperature germination of *Pteris vittata* seeds, characterized in that, The method includes the following steps: S1. Select plump, insect-free, undamaged, and well-developed yellow-flowered pig manure bean seeds and disinfect them to obtain disinfected seeds for later use. S2. The disinfected seeds are placed in a water bath at 0-5℃ and pressurized to 0.2-0.3 MPa for 10-20 minutes. Then, the pressure is restored to normal and the seeds are placed in warm water at 40-45℃ for 5-10 minutes to obtain pretreated seeds for later use. S3. Add the pretreated seeds to a starch solution and sonicate them, then wash them clean, soak them in water and set aside. S4. Germinate the soaked seeds at high temperature.
2. The method for promoting high-temperature germination of *Pteris vittata* seeds according to claim 1, characterized in that: In step S1, the seeds of the yellow-flowered pig manure bean are disinfected by soaking them in a 0.1% potassium permanganate solution for 5 minutes, followed by repeated rinsing with clean water.
3. The method for promoting high-temperature germination of *Pteris vittata* seeds according to claim 1, characterized in that: In step S2, the temperature difference between the two treatments at 0-5℃ and 40-45℃ is ≤40℃.
4. The method for promoting high-temperature germination of *Pteris vittata* seeds according to claim 1, characterized in that: In step S3, the mass concentration of the starch solution is 20%-30%.
5. The method for promoting high-temperature germination of *Pteris vittata* seeds according to claim 4, characterized in that: The starch solution also contains 200 mmol / L of gibberellin.
6. The method for promoting high-temperature germination of *Pteris vittata* seeds according to claim 1, characterized in that: In step S3, the ultrasonic treatment is performed at a power of 400-600W for 3-5 minutes, followed by resting for 5-8 minutes, and then continuing ultrasonic treatment for another 3-5 minutes. This process is repeated until the total ultrasonic time is 15-25 minutes.
7. The method for promoting high-temperature germination of *Pteris vittata* seeds according to claim 1, characterized in that: The soaking time in clean water in step S3 is 30-40 minutes.
8. The method for promoting high-temperature germination of *Pteris vittata* seeds according to claim 1, characterized in that: In step S4, the temperature for high-temperature germination is 30-38℃, and the ambient humidity during germination is 80%-90%.
9. The method for promoting high-temperature germination of *Pteris vittata* seeds according to claim 1, characterized in that: In step S4, the light conditions for high-temperature germination are a 12-hour light / 12-hour dark cycle, and the light intensity is 2000 lx.