Hippocampus live biological bait culture method and application thereof

Abstract

This invention relates to the field of marine aquaculture technology, and discloses a method for cultivating live seahorse feed and its application. The method includes placing broodstock Oriental white shrimp carrying eggs in an indoor culture tank, feeding, wastewater removal, and water exchange under incubation conditions to obtain first-stage zoea larvae of Oriental white shrimp; continuously cultivating the first-stage zoea larvae to juvenile shrimp; further cultivating the juvenile shrimp by feeding them Artemia nauplii and shrimp starter feed to obtain juvenile shrimp with a total length ≤3cm. The live seahorse feed of this invention is nutritious, can be bred and cultivated year-round, is easily consumed by seahorses, and can achieve good aquaculture results. Based on the feeding habits of seahorses of different sizes, it provides zoea larvae, mysid larvae, and juvenile shrimp at different developmental stages, exhibiting good palatability and avoiding nutritional deficiencies and large-scale disease outbreaks during seahorse farming. It also avoids the high mortality rate caused by the acclimatization process of using frozen feed, thus improving the survival rate of seahorse farming.

Description

Technical Field

[0001] This invention relates to the field of marine aquaculture technology, and more specifically, to a method for cultivating live seahorse feed and its application. Background Technology

[0002] Seahorse (Hippocampus) is a precious traditional Chinese medicine with effects such as nourishing essence and qi, and promoting blood circulation and removing blood stasis. Due to habitat destruction and overfishing caused by strong market demand, wild seahorse resources have decreased, and artificial breeding has become an important means to solve resource scarcity and meet market demand.

[0003] Currently, seahorse farming primarily uses live small planktonic organisms as initial feed, including rotifers, copepods, and Artemia nauplii. During the rearing stage, frozen amphipods, mysids, krill, and Artemia (brine shrimp) are mainly fed. However, frozen feed suffers from inconsistent quality and difficulty in adding nutritional enhancers, immune boosters, and disease control agents during the rearing stage. Long-term use of a single frozen feed can lead to nutritional deficiencies, decreased growth rate, and low survival rate in seahorses. Existing live feeds, while readily available and stable, include mysids and krill, which exhibit significant seasonality and inconsistent size distribution. Furthermore, these live feeds are difficult to adapt to artificial feeds, hindering the addition of functional ingredients such as nutritional enhancers, immune boosters, and disease control agents. Therefore, developing a system capable of supplying live feeds of varying sizes and suitable for cultivation with artificial feeds would be ideal for seahorse nutritional fortification, immune enhancement, and disease control, improving growth rate and survival rate, and promoting the development of the seahorse farming industry.

[0004] No effective solutions have yet been proposed to address the problems in the relevant technologies. Summary of the Invention

[0005] In response to the problems in related technologies, this invention proposes a method for cultivating live seahorse food and its application, in order to overcome the aforementioned technical problems existing in the existing related technologies.

[0006] Therefore, the specific technical solution adopted by the present invention is as follows:

[0007] According to one aspect of the present invention, a method for culturing live seahorse food is provided, comprising:

[0008] Parent shrimp carrying eggs were placed in an indoor culture pond, and feeding, waste disposal and water exchange were carried out under incubation conditions to obtain the first-stage zoea larvae of Oriental white shrimp.

[0009] The first-stage zoea larvae were continuously cultured to the first-stage juvenile shrimp;

[0010] The first-stage larvae were further cultured and fed Artemia nauplii and shrimp starter feed to obtain larvae with a total length of ≤3cm.

[0011] Preferably, the incubation conditions are: temperature of 15℃-33℃, salinity of 5‰-35‰, dissolved oxygen ≥5mg / L, and pH value of 7.5-9.0 in the indoor culture tank.

[0012] Preferably, broodstock Oriental white shrimp carrying eggs are placed in an indoor culture pond, and feeding, waste disposal, and water exchange are carried out under incubation conditions to obtain the first-stage zoea larvae of Oriental white shrimp, including:

[0013] Select egg-bearing Oriental white shrimp broodstock from farmed shrimp, at a ratio of 50 shrimp / m 3 -100 tails / m 3 The density was adjusted and the fish were placed in an indoor aquaculture pond with a water depth of 0.8m-1.2m.

[0014] Feed the adult shrimp with eggs at 6-8% of their body weight with formulated shrimp feed. Divide the formulated shrimp feed into 4:2:4 ratios and feed them three times a day at 6:00-7:00, 11:00-12:00 and 17:00-18:00 respectively. Before feeding, use a siphon to remove uneaten feed and feces.

[0015] The water is changed daily at 4 PM, with 1 / 3 to 1 / 2 of the water being replaced. After 15 to 20 days at a temperature between 15℃ and 30℃, the first-stage zoea larvae are obtained.

[0016] Preferably, continuously culturing stage 1 zoea larvae to stage 1 postlarvae includes:

[0017] First-stage zoea larvae with a total length of 3.0mm-3.5mm were selected for cultivation and fed with *Phaeodactylum tricornutum* or *Chaetoceros*. After 1-2 days of metamorphosis, they developed into second-stage zoea larvae. The cultivation density was 100,000-150,000 larvae / m². 3 The food density is 300,000-400,000 cells / ml;

[0018] Second-stage zoea larvae, with a total length of 3.3mm-3.7mm, were selected for cultivation and fed with *Phaeodactylum tricornutum* or *Chaetoceros*, supplemented with *Brachycota foldis*. After 1-2 days of metamorphosis, they developed into first-stage mysid larvae. The cultivation density was 100,000-150,000 larvae / m². 3 The food density was 200,000 cells / ml, and the Brachiopoda foldis density was 15 cells / ml.

[0019] First-stage mysid larvae with a total length of 3.6mm-3.9mm were selected for rearing and fed with Brachionus rotifer and Artemia nauplii. After 2-3 days of metamorphosis, they developed into second-stage mysid larvae. The rearing density was 100,000-150,000 larvae / m². 3 Brachiopoda foldis was 15 cells / ml, and Artemia nauplii were 5 cells / ml.

[0020] Second-stage mysid larvae with a total length of 4.0mm-5.0mm were selected for rearing, fed with Artemia nauplii and shrimp microparticle feed, and metamorphosed into third-stage mysid larvae in 2-3 days; the rearing density was 80,000-120,000 larvae / m². 3 The concentration of Artemia nauplii was 5 per ml, and the proportion of microparticle feed for shrimp was 0.06 kg per 10,000 mysid larvae.

[0021] Third-stage mysid shrimp larvae with a total length of 5.0mm-5.6mm were selected for rearing, fed with Artemia nauplii and shrimp microparticle feed, and metamorphosed into first-stage larvae in 3-4 days; the rearing density was 80,000-120,000 shrimp / m². 3 The concentration of Artemia nauplii was 5 per ml, and the ratio of microparticle feed to shrimp was 0.10 kg per 10,000 mysid larvae.

[0022] Preferably, the first-stage larvae are further cultured and fed Artemia nauplii and shrimp starter feed to obtain juvenile shrimp with a total length of ≤3cm, including:

[0023] First-stage larvae with a total length of 5.2-5.8 mm were selected and fed with Artemia nauplii and shrimp starter feed. After 1-2 days, they grew into second-stage larvae; the rearing density was 50,000-100,000 larvae / m². 3 The concentration of Artemia nauplii was 5 per ml, and the ratio of the initial feed for shrimp was 0.10 kg / 10,000 juvenile shrimp.

[0024] Second-stage larvae with a total length of 5.7 mm or more were selected and fed Artemia nauplii and shrimp starter feed. After 10-20 days, they entered the juvenile stage; the rearing density was 50,000-100,000 shrimp / m². 3 The concentration of Artemia nauplii was 5 per ml, and the ratio of the initial feed for shrimp was 0.10 kg per 10,000 juvenile shrimp.

[0025] Preferably, nutritional enhancers and / or immune enhancers are added to shrimp microparticle feed and / or shrimp starter feed.

[0026] Preferably, the nutritional enhancer is fish oil, which is added to shrimp microparticle feed and / or shrimp starter feed to provide nutritional fortification for multi-stage zoea larvae, mysid larvae and postlarvae.

[0027] Preferably, the immune enhancer is β-glucan, which is added to shrimp microparticle feed and / or shrimp starter feed to enhance the immunity of multi-stage zoea larvae, mysid larvae and postlarvae.

[0028] According to another aspect of the present invention, an application of live seahorse food is provided, comprising:

[0029] Zodiacal larvae, mysid larvae, and shrimp juveniles were removed from the rearing pond, disinfected, and then fed to seahorses. The corresponding body lengths of the seahorses fed to zodiacal larvae, mysid larvae, and shrimp juveniles at different developmental stages were as follows:

[0030] Feed the first-stage zoea larvae with seahorses that are 3.0cm-3.5cm in length;

[0031] Second-stage zoea larvae were fed seahorses with a body length of 3.5cm-4.0cm;

[0032] First-stage mysid larvae were fed seahorses with a body length of 4.0cm-4.5cm;

[0033] Second-stage mysid larvae were fed seahorses with a body length of 4.5cm-5.0cm;

[0034] Third-stage mysid larvae were fed seahorses with a body length of 5.0cm-5.5cm;

[0035] First-stage larvae were fed seahorses with a body length of 5.5cm-6.0cm;

[0036] Juvenile shrimp with a body length of 6mm-10mm were fed seahorses with a body length of 6.0cm-8.0cm;

[0037] Juvenile shrimp with a body length of 10mm-20mm were fed seahorses with a body length of 8.0cm-10.0cm;

[0038] Juvenile shrimp with a body length of 20mm-30mm were fed seahorses with a body length of >10cm.

[0039] Preferably, the process of removing zoea larvae, mysid larvae, and baby shrimp at different developmental stages from the culture pond, disinfecting them, and then feeding them to the seahorse includes:

[0040] Disinfect zoea larvae, mysid larvae, and juvenile shrimp at different developmental stages by immersing them in seawater containing a 10 mg / L-20 mg / L povidone-iodine solution for 10-20 minutes. After disinfection, rinse them 1-2 times with clean seawater and allow them to recover in oxygenated clean seawater for 1-3 minutes before feeding them to seahorses.

[0041] The beneficial effects of this invention are as follows:

[0042] 1. The live seahorse feed of this application is rich in nutrients, can be bred and cultivated in all seasons, is easy for seahorses to eat, and can achieve good breeding results.

[0043] 2. Based on the feeding habits of seahorses of different sizes, we provide zoea larvae, mysid larvae, and juvenile shrimp at different developmental stages, which have good palatability. We also use additives to enhance nutrition, immunity, and disease control, so as to avoid nutritional deficiencies and large-scale disease outbreaks during seahorse farming.

[0044] 3. The live feed in this application can be fed to newly hatched seahorses after they have been raised to a body length of 3.0 cm using copepod and brine shrimp larvae. This allows for the use of live feed throughout the entire breeding process, avoiding the high mortality rate caused by the acclimatization process of using frozen feed and significantly improving the survival rate of seahorse breeding. Attached Figure Description

[0045] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0046] Figure 1 This is a flowchart of a method for cultivating live seahorse food according to an embodiment of the present invention. Detailed Implementation

[0047] To further illustrate the various embodiments, the present invention provides accompanying drawings, which are part of the disclosure of the present invention. These drawings are mainly used to illustrate the embodiments and can be used in conjunction with the relevant descriptions in the specification to explain the operating principles of the embodiments. With reference to these drawings, those skilled in the art should be able to understand other possible implementation methods and the advantages of the present invention. The components in the drawings are not drawn to scale, and similar component symbols are generally used to represent similar components.

[0048] According to an embodiment of the present invention, a method for cultivating live seahorse food and its application are provided.

[0049] The present invention will now be further described in conjunction with the accompanying drawings and specific embodiments, such as... Figure 1 As shown, a method for cultivating live seahorse food according to an embodiment of the present invention includes:

[0050] Parent shrimp carrying eggs were placed in an indoor culture pond, and feeding, waste disposal and water exchange were carried out under incubation conditions to obtain the first-stage zoea larvae of Oriental white shrimp.

[0051] The first-stage zoea larvae were continuously cultured to the first-stage juvenile shrimp;

[0052] The first-stage larvae were further cultured and fed Artemia nauplii and shrimp starter feed to obtain larvae with a total length of ≤3cm.

[0053] Preferably, the incubation conditions are: temperature of 15℃-33℃, salinity of 5‰-35‰, dissolved oxygen ≥5mg / L, and pH value of 7.5-9.0 in the indoor culture tank.

[0054] As a preferred embodiment, broodstock Oriental white shrimp carrying eggs are placed in an indoor culture pond, and feeding, waste disposal, and water exchange are carried out under incubation conditions to obtain the first-stage zoea larvae of Oriental white shrimp, including:

[0055] Select egg-bearing Oriental white shrimp broodstock from farmed shrimp, at a ratio of 50 shrimp / m 3 -100 tails / m 3 The density was adjusted and the fish were placed in an indoor aquaculture pond with a water depth of 0.8m-1.2m.

[0056] Feed the adult shrimp with eggs at 6-8% of their body weight with formulated shrimp feed. Divide the formulated shrimp feed into 4:2:4 ratios and feed them three times a day at 6:00-7:00, 11:00-12:00 and 17:00-18:00 respectively. Before feeding, use a siphon to remove uneaten feed and feces.

[0057] The water is changed daily at 4 PM, with 1 / 3 to 1 / 2 of the water being replaced. After 15 to 20 days at a temperature between 15℃ and 30℃, the first-stage zoea larvae are obtained.

[0058] Preferably, continuously culturing stage 1 zoea larvae to stage 1 postlarvae includes:

[0059] First-stage zoea larvae with a total length of 3.0mm-3.5mm were selected for cultivation and fed with *Phaeodactylum tricornutum* or *Chaetoceros*. After 1-2 days of metamorphosis, they developed into second-stage zoea larvae. The cultivation density was 100,000-150,000 larvae / m². 3 The food density is 300,000-400,000 cells / ml;

[0060] Second-stage zoea larvae, with a total length of 3.3mm-3.7mm, were selected for cultivation and fed with *Phaeodactylum tricornutum* or *Chaetoceros*, supplemented with *Brachycota foldis*. After 1-2 days of metamorphosis, they developed into first-stage mysid larvae. The cultivation density was 100,000-150,000 larvae / m². 3 The food density was 200,000 cells / ml, and the Brachiopoda foldis density was 15 cells / ml.

[0061] First-stage mysid larvae with a total length of 3.6mm-3.9mm were selected for rearing and fed with Brachionus rotifer and Artemia nauplii. After 2-3 days of metamorphosis, they developed into second-stage mysid larvae. The rearing density was 100,000-150,000 larvae / m². 3 Brachiopoda foldis was 15 cells / ml, and Artemia nauplii were 5 cells / ml.

[0062] Second-stage mysid larvae with a total length of 4.0mm-5.0mm were selected for rearing, fed with Artemia nauplii and shrimp microparticle feed, and metamorphosed into third-stage mysid larvae in 2-3 days; the rearing density was 80,000-120,000 larvae / m². 3The concentration of Artemia nauplii was 5 per ml, and the proportion of microparticle feed for shrimp was 0.06 kg per 10,000 mysid larvae.

[0063] Third-stage mysid shrimp larvae with a total length of 5.0mm-5.6mm were selected for rearing, fed with Artemia nauplii and shrimp microparticle feed, and metamorphosed into first-stage larvae in 3-4 days; the rearing density was 80,000-120,000 shrimp / m². 3 The concentration of Artemia nauplii was 5 per ml, and the ratio of microparticle feed to shrimp was 0.10 kg per 10,000 mysid larvae.

[0064] Preferably, the first-stage larvae are further cultured and fed Artemia nauplii and shrimp starter feed to obtain juvenile shrimp with a total length of ≤3cm, including:

[0065] First-stage larvae with a total length of 5.2-5.8 mm were selected and fed with Artemia nauplii and shrimp starter feed. After 1-2 days, they grew into second-stage larvae; the rearing density was 50,000-100,000 larvae / m². 3 The concentration of Artemia nauplii was 5 per ml, and the ratio of the initial feed for shrimp was 0.10 kg / 10,000 juvenile shrimp.

[0066] Second-stage larvae with a total length of 5.7 mm or more were selected and fed Artemia nauplii and shrimp starter feed. After 10-20 days, they entered the juvenile stage; the rearing density was 50,000-100,000 shrimp / m². 3 The concentration of Artemia nauplii was 5 per ml, and the ratio of the initial feed for shrimp was 0.10 kg per 10,000 juvenile shrimp.

[0067] As a preferred embodiment, nutritional enhancers and / or immune enhancers are added to shrimp microparticle feed and / or shrimp starter feed.

[0068] As a preferred embodiment, the nutritional enhancer is fish oil, which is added to shrimp microparticle feed and / or shrimp starter feed to nutritionally enhance multi-stage zoea larvae, mysid larvae and postlarvae.

[0069] As a preferred embodiment, the immune enhancer is β-glucan, which is added to shrimp microparticle feed and / or shrimp starter feed to enhance the immunity of multi-stage zoea larvae, mysid larvae and juvenile shrimp.

[0070] In addition, once the juvenile shrimp enter the larval stage, the rearing environment is transferred to cement ponds, earthen ponds, or mulched ponds, with a stocking density of 200-500 shrimp / m². 2Feed the shrimp with shrimp feed (0.5mm diameter pellets). The feeding amount is 1.0kg / 10,000 shrimp when the shrimp are 3cm, 4cm, and 5cm in length, 1.5kg / 10,000 shrimp when they are 10,000 shrimp when they are 3cm, 10,000 shrimp when they are 4cm, and 2.25kg / 10,000 shrimp when they are 5cm in length. The feeding amount is divided into three feedings in a ratio of 4:2:4. Feed the shrimp at 6:00-7:00, 11:00-12:00 and 17:00-18:00 respectively. Discharge the sewage in the aquaculture pond 30 minutes before feeding if the pond has sewage discharge facilities.

[0071] Broodstock shrimp are sexually mature individuals obtained through further rearing of juvenile shrimp. They are used for mating, carrying eggs, and hatching to produce first-stage zoea larvae. The rearing environment for broodstock shrimp is also cement ponds, earthen ponds, or film-lined ponds, with a stocking density of 100-200 shrimp / m². 2 Feed shrimp with a vitamin-added feed (particle diameter 1mm) at a rate of 3kg / 10,000 shrimp, divided into 3 feedings in a 4:2:4 ratio, at 6:00-7:00, 11:00-12:00, and 17:00-18:00 respectively. Discharge the wastewater in the aquaculture pond 30 minutes before feeding.

[0072] According to another embodiment of the present invention, an application of live seahorse food is provided, comprising:

[0073] Zoea larvae, mysid larvae, and shrimp juveniles at different developmental stages were removed from the rearing pond, disinfected, and then fed to seahorses. The corresponding body lengths of the seahorses fed to the zoea larvae, mysid larvae, and shrimp juveniles at different developmental stages were as follows:

[0074] Feed the first-stage zoea larvae with seahorses that are 3.0cm-3.5cm in length;

[0075] Second-stage zoea larvae were fed seahorses with a body length of 3.5cm-4.0cm;

[0076] First-stage mysid larvae were fed seahorses with a body length of 4.0cm-4.5cm;

[0077] Second-stage mysid larvae were fed seahorses with a body length of 4.5cm-5.0cm;

[0078] Third-stage mysid larvae were fed seahorses with a body length of 5.0cm-5.5cm;

[0079] First-stage larvae were fed seahorses with a body length of 5.5cm-6.0cm;

[0080] Juvenile shrimp with a body length of 6mm-10mm were fed seahorses with a body length of 6.0cm-8.0cm;

[0081] Juvenile shrimp with a body length of 10mm-20mm were fed seahorses with a body length of 8.0cm-10.0cm;

[0082] Juvenile shrimp with a body length of 20mm-30mm were fed seahorses with a body length of >10cm.

[0083] Preferably, the process of removing zoea larvae, mysid larvae, and baby shrimp at different developmental stages from the culture pond, disinfecting them, and then feeding them to the seahorse includes:

[0084] Disinfect zoea larvae, mysid larvae, and juvenile shrimp at different developmental stages by immersing them in seawater containing a 10 mg / L-20 mg / L povidone-iodine solution for 10-20 minutes. After disinfection, rinse them 1-2 times with clean seawater and allow them to recover in oxygenated clean seawater for 1-3 minutes before feeding them to seahorses.

[0085] The specific implementation of the present invention will be further described in detail below with reference to examples:

[0086] Example 1

[0087] This embodiment provides a method for cultivating live seahorse feed. Egg-bearing parent shrimp of the Oriental white shrimp are placed in an indoor culture tank. Under incubation conditions, feeding, waste removal, and water exchange are performed to obtain the first-stage zoea larvae of the Oriental white shrimp. The first-stage zoea larvae are continuously cultured to the first-stage juvenile shrimp. The first-stage juvenile shrimp are further cultured, fed with Artemia nauplii and shrimp starter feed, to obtain juvenile shrimp with a total length ≤3cm.

[0088] Select broodstock shrimp that are carrying eggs (eggs are pale yellow or pale green) from the cultured shrimp population, at a rate of 50 shrimp / m². 3 -100 tails / m 3 The shrimp were placed in indoor rearing ponds at a density of 0.8m-1.2m. They were fed a formulated shrimp feed daily at a ratio of 6-8% of their body weight, divided into three feedings in a 4:2:4 ratio: 6:00-7:00, 11:00-12:00, and 17:00-18:00. Uneaten feed and feces were removed using a siphon before each feeding. One-third to one-half of the water was changed daily at 16:00. At a temperature of 15-30℃, the first-stage zoea larvae hatched in 15-20 days.

[0089] First-stage zoea larvae with a total length of 3.0mm-3.5mm were selected for cultivation and fed with *Phaeodactylum tricornutum* or *Chaetoceros*. After 1-2 days of metamorphosis, they developed into second-stage zoea larvae. The cultivation density was 100,000-150,000 larvae / m². 3 The food density is 300,000-400,000 cells / ml.

[0090] Second-stage zoea larvae, with a total length of 3.3mm-3.7mm, were selected for cultivation and fed with *Phaeodactylum tricornutum* or *Chaetoceros*, supplemented with *Brachycota foldis*. After 1-2 days of metamorphosis, they developed into first-stage mysid larvae. The cultivation density was 100,000-150,000 larvae / m². 3 The food density was 200,000 cells / ml, and the density of Brachiopoda foldis was 15 cells / ml.

[0091] First-stage mysid larvae with a total length of 3.6mm-3.9mm were selected for rearing and fed with Brachionus rotifer and Artemia nauplii. After 2-3 days of metamorphosis, they developed into second-stage mysid larvae. The rearing density was 100,000-150,000 larvae / m². 3 The concentration of Brachiopoda foldis was 15 per ml, and the concentration of Artemia nauplii was 5 per ml.

[0092] Second-stage mysid larvae with a total length of 4.0mm-5.0mm were selected for rearing, fed with Artemia nauplii and shrimp microparticle feed, and metamorphosed into third-stage mysid larvae in 2-3 days; the rearing density was 80,000-120,000 larvae / m². 3 The concentration of Artemia nauplii was 5 per ml, and the ratio of microparticle feed to shrimp was 0.06 kg per 10,000 mysid larvae.

[0093] Third-stage mysid shrimp larvae with a total length of 5.0mm-5.6mm were selected for rearing, fed with Artemia nauplii and shrimp microparticle feed, and metamorphosed into first-stage larvae in 3-4 days; the rearing density was 80,000-120,000 shrimp / m². 3 The concentration of Artemia nauplii was 5 per ml, and the ratio of microparticle feed to shrimp was 0.10 kg per 10,000 mysid larvae.

[0094] First-stage larvae with a total length of 5.2-5.8 mm were selected and fed with Artemia nauplii and shrimp starter feed. After 1-2 days, they grew into second-stage larvae; the rearing density was 50,000-100,000 larvae / m². 3 The concentration of Artemia nauplii was 5 per ml, and the ratio of the initial feed for shrimp was 0.10 kg per 10,000 juvenile shrimp.

[0095] Second-stage larvae with a total length of 5.7 mm or more were selected and fed Artemia nauplii and shrimp starter feed. After 10-20 days, they entered the juvenile stage; the rearing density was 50,000-100,000 shrimp / m². 3 The concentration of Artemia nauplii was 5 per ml, and the ratio of the initial feed for shrimp was 0.10 kg per 10,000 juvenile shrimp.

[0096] It should be noted that during the rearing of stage 3 mysid larvae, stage 1 larvae, and larvae, the amount of feed was provided according to the proportion of stage 1 zoea larvae.

[0097] In addition, the particle diameter of shrimp micro-feed is 0.05 mm, and the particle diameter of shrimp starter feed is 0.1 mm.

[0098] Example 2

[0099] This embodiment provides a method for cultivating live seahorse feed. Egg-bearing parent shrimp of the Oriental white shrimp are placed in an indoor culture tank. Under incubation conditions, feeding, waste removal, and water exchange are performed to obtain the first-stage zoea larvae of the Oriental white shrimp. The first-stage zoea larvae are continuously cultured to the first-stage juvenile shrimp. The first-stage juvenile shrimp are further cultured, fed with Artemia nauplii and shrimp starter feed, to obtain juvenile shrimp with a total length ≤3cm.

[0100] Select broodstock shrimp that are carrying eggs (eggs are pale yellow or pale green) from the cultured shrimp population, at a rate of 50 shrimp / m². 3 -100 tails / m 3 Place the broodstock shrimp in indoor rearing ponds with a water depth of 0.8m-1.2m. Feed them daily with a shrimp feed containing 5% micro-particle fish oil, at a ratio of 6-8% of their body weight. Divide the daily feed into three feedings in a 4:2:4 ratio: 6:00-7:00, 11:00-12:00, and 17:00-18:00. Before feeding, remove uneaten feed and feces using a siphon. Change 1 / 3-1 / 2 of the water daily at 16:00. At a temperature of 15-30℃, the first-stage zoea larvae will hatch in 15-20 days.

[0101] First-stage zoea larvae with a total length of 3.0mm-3.5mm were selected for cultivation and fed with *Phaeodactylum tricornutum* or *Chaetoceros*, supplemented with 0.1g / m³ of *Phaeodactylum tricornutum*. 3 Microparticle fish oil undergoes metamorphosis into stage 2 zoea larvae in 1-2 days; the culture density is 100,000-150,000 fish / m². 3 The food density is 300,000-400,000 cells / ml.

[0102] Second-stage zoea larvae with a total length of 3.3mm-3.7mm were selected for cultivation. They were fed with *Phaeodactylum tricornutum* or *Chaetoceros*, supplemented with *Brachycota foldis* and 0.1g / m³ of other organisms. 3 Microparticle fish oil, after 1-2 days of metamorphosis into stage 1 mysid shrimp larvae; the culture density is 100,000-150,000 shrimp / m³. 3 The food density was 200,000 cells / ml, and the density of Brachiopoda foldis was 15 cells / ml.

[0103] First-stage mysid shrimp larvae with a total length of 3.6mm-3.9mm were selected for rearing and fed with Brachionus rotifer, Artemia nauplii, and supplemented with 0.1g / m 3 Microparticle fish oil, after 2-3 days of metamorphosis into stage 2 mysid shrimp larvae; the rearing density is 100,000-150,000 shrimp / m³. 3 The concentration of Brachiopoda foldis was 15 per ml, and the concentration of Artemia nauplii was 5 per ml.

[0104] Second-stage mysid larvae with a total length of 4.0mm-5.0mm were selected for rearing. They were fed Artemia nauplii and shrimp microparticle feed (0.05mm in diameter), with microparticle fish oil added at 5% of the shrimp feed. After 2-3 days of metamorphosis, they developed into third-stage mysid larvae. The rearing density was 80,000-120,000 larvae / m². 3 The concentration of Artemia nauplii was 5 per ml, and the ratio of microparticle feed to shrimp was 0.06 kg per 10,000 mysid larvae.

[0105] Third-stage mysid larvae with a total length of 5.0mm-5.6mm were selected for cultivation. They were fed Artemia nauplii and shrimp microparticle feed (particle diameter 0.05mm), and micro-particle fish oil was added at 5% of the shrimp feed. After 3-4 days, they metamorphosed into first-stage larvae. The cultivation density was 80,000-120,000 larvae / m3, the Artemia nauplii ratio was 5 nauplii / ml, and the proportion of shrimp microparticle feed was 0.10kg / 10,000 mysid larvae.

[0106] First-stage larvae with a total length of 5.2-5.8 mm were selected and fed Artemia nauplii and shrimp starter feed (0.1 mm in diameter). Micro-particle fish oil was added at 5% of the shrimp feed. After 1-2 days, they grew into second-stage larvae. The rearing density was 50,000-100,000 larvae / m². 3 The concentration of Artemia nauplii was 5 per ml, and the ratio of the initial feed for shrimp was 0.10 kg per 10,000 juvenile shrimp.

[0107] Second-stage larvae with a total length of 5.7 mm or more were selected and fed Artemia nauplii and shrimp starter feed (0.1 mm in diameter). Micro-particle fish oil was added at 5% of the shrimp feed. After 10-20 days, the shrimp entered the juvenile stage. The rearing density was 50,000-100,000 shrimp / m². 3 The concentration of Artemia nauplii was 5 per ml, and the ratio of the initial feed for shrimp was 0.10 kg per 10,000 juvenile shrimp.

[0108] It should be noted that during the rearing of third-stage mysid larvae, first-stage juvenile shrimp, and juvenile shrimp, the feed amount was the same as that for first-stage zoea larvae.

[0109] In addition, the particle diameter of shrimp micro-feed is 0.05 mm, and the particle diameter of shrimp starter feed is 0.1 mm.

[0110] Example 3

[0111] This embodiment provides a method for cultivating live seahorse feed. Egg-bearing parent shrimp of the Oriental white shrimp are placed in an indoor culture tank. Under incubation conditions, feeding, waste removal, and water exchange are performed to obtain the first-stage zoea larvae of the Oriental white shrimp. The first-stage zoea larvae are continuously cultured to the first-stage juvenile shrimp. The first-stage juvenile shrimp are further cultured, fed with Artemia nauplii and shrimp starter feed, to obtain juvenile shrimp with a total length ≤3cm.

[0112] Select broodstock shrimp that are carrying eggs (eggs are pale yellow or pale green) from the cultured shrimp population, at a rate of 50 shrimp / m². 3 -100 tails / m 3 The shrimp were placed in indoor rearing ponds at a density of 0.8m-1.2m. They were fed daily with a shrimp feed supplemented with 5% β-glucan, at a ratio of 6-8% of their body weight. The daily feed amount was divided into three feedings in a 4:2:4 ratio: 6:00-7:00, 11:00-12:00, and 17:00-18:00. Uneaten feed and feces were removed using a siphon before each feeding. One-third to one-half of the water was changed daily at 16:00. At a temperature of 15-30℃, the first-stage zoea larvae hatched in 15-20 days.

[0113] First-stage zoea larvae with a total length of 3.0 mm-3.5 mm were selected for cultivation and fed with *Phaeodactylum tricornutum* or *Chaetoceros* at a concentration of 0.1 g / m³. 3 Shrimp were fed microparticle feed supplemented with 5% β-glucan at a ratio that resulted in metamorphosis into stage 2 zoea larvae after 1-2 days; the rearing density was 100,000-150,000 shrimp / m². 3 The food density is 300,000-400,000 cells / ml.

[0114] Second-stage zoea larvae with a total length of 3.3mm-3.7mm were selected for cultivation. They were fed with *Phaeodactylum tricornutum* or *Chaetoceros* as food, supplemented with *Brachycota foldis* and 0.1g / m³ of other organisms. 3 Shrimp were fed microparticle feed supplemented with 5% β-glucan at a ratio of 1:10:00 to 150,000 shrimp / m². After 1-2 days of metamorphosis, they developed into first-stage mysid larvae. 3 The food density was 200,000 cells / ml, and the density of Brachiopoda foldis was 15 cells / ml.

[0115] First-stage mysid larvae with a total length of 3.6mm-3.9mm were selected for rearing. They were fed with Brachiosaurus foldis, Artemia nauplii, and shrimp microparticle feed supplemented with 5% β-glucan at a ratio of 0.1g / m3. After 2-3 days of metamorphosis, they developed into second-stage mysid larvae. The rearing density was 100,000-150,000 larvae / m3. 3 The concentration of Brachiopoda foldis was 15 per ml, and the concentration of Artemia nauplii was 5 per ml.

[0116] Second-stage mysid shrimp larvae with a total length of 4.0mm-5.0mm were selected for rearing. They were fed Artemia nauplii and shrimp microparticle feed (0.05mm in diameter) supplemented with 5% β-glucan at a rate of 0.06kg / 10,000 larvae, with an additional 0.1g / m 3 Shrimp were fed microparticle feed supplemented with 5% β-glucan at a ratio of 2-3 days, and after 2-3 days, they metamorphosed into stage 3 mysid larvae; the rearing density was 80,000-120,000 shrimp / m². 3The concentration of Artemia nauplii was 5 per ml, and the ratio of microparticle feed to shrimp was 0.06 kg per 10,000 mysid larvae.

[0117] Third-stage mysid larvae with a total length of 5.0mm-5.6mm were selected for cultivation. They were fed Artemia nauplii and shrimp microparticle feed (0.1mm diameter) supplemented with 5% β-glucan at a rate of 0.10kg / 10,000 larvae. After 3-4 days, they metamorphosed into first-stage juvenile shrimp. The cultivation density was 80,000-120,000 larvae / m3, the Artemia nauplii rate was 5 nauplii / ml, and the proportion of shrimp microparticle feed was 0.10kg / 10,000 mysid larvae.

[0118] First-stage shrimp with a total length of 5.2-5.8 mm were selected and fed Artemia nauplii and shrimp microparticle feed (0.1 mm in diameter) supplemented with 5% β-glucan at a rate of 0.10 kg / 10,000 shrimp. The shrimp underwent metamorphosis in 1-2 days; the rearing density was 50,000-100,000 shrimp / m². 3 The concentration of Artemia nauplii was 5 per ml, and the ratio of the initial feed for shrimp was 0.10 kg per 10,000 juvenile shrimp.

[0119] Second-stage larvae with a total length of 5.7 mm or more were selected and fed Artemia nauplii and shrimp microparticle feed (0.1 mm in diameter) supplemented with 5% β-glucan at a rate of 0.10 kg / 10,000 larvae. After 10-20 days, they entered the juvenile stage; the rearing density was 50,000-100,000 larvae / m². 3 The concentration of Artemia nauplii was 5 per ml, and the ratio of the initial feed for shrimp was 0.10 kg per 10,000 juvenile shrimp.

[0120] It should be noted that during the rearing of third-stage mysid larvae, first-stage juvenile shrimp, and juvenile shrimp, the feed amount was the same as that for first-stage zoea larvae.

[0121] In addition, the particle diameter of shrimp micro-feed is 0.05 mm, and the particle diameter of shrimp starter feed is 0.1 mm.

[0122] Example 4

[0123] This embodiment provides an application of live seahorse feed. Zodiac larvae, mysid larvae, and shrimp larvae cultivated in Example 1 are removed from the culture pond, disinfected, and then fed to the seahorses. The corresponding body lengths of the seahorses fed with zodiac larvae, mysid larvae, and shrimp larvae at different developmental stages are as follows:

[0124] Feed seahorses with a body length of 3.0cm-3.5cm to stage 1 zoea larvae; 3.5cm-4.0cm to stage 2 zoea larvae; 4.0cm-4.5cm to stage 1 mysid larvae; 4.5cm-5.0cm to stage 2 mysid larvae; 5.0cm-5.5cm to stage 3 mysid larvae; 5.5cm-6.0cm to stage 1 postlarvae; 6.0cm-8.0cm to stage 6mm-10mm to stage 6.0cm-10.0cm to stage 6.0cm-20mm to stage 6.0cm-10.0cm to stage 6.0cm-30mm to stage 20mm-30mm to stage 20mm-30mm to stage 20cm-30cm ...

[0125] Before feeding, disinfect the zoea larvae, mysid larvae, and juvenile shrimp by soaking them in seawater containing a 10mg / L-20mg / L povidone-iodine solution for 10-20 minutes. After disinfection, rinse them 1-2 times with clean seawater and allow them to recover in oxygenated clean seawater for 1-3 minutes before feeding them to the seahorses.

[0126] Example 5

[0127] This embodiment provides an application of live seahorse feed. Zodiac larvae, mysid larvae, and shrimp larvae cultivated in Example 2 are removed from the culture pond, disinfected, and then fed to the seahorses. The corresponding body lengths of the seahorses fed with zodiac larvae, mysid larvae, and shrimp larvae at different developmental stages are as follows:

[0128] Feed seahorses with a body length of 3.0cm-3.5cm to stage 1 zoea larvae; 3.5cm-4.0cm to stage 2 zoea larvae; 4.0cm-4.5cm to stage 1 mysid larvae; 4.5cm-5.0cm to stage 2 mysid larvae; 5.0cm-5.5cm to stage 3 mysid larvae; 5.5cm-6.0cm to stage 1 postlarvae; 6.0cm-8.0cm to stage 6mm-10mm to stage 6.0cm-10.0cm to stage 6.0cm-20mm to stage 6.0cm-10.0cm to stage 6.0cm-30mm to stage 20mm-30mm to stage 20mm-30mm to stage 20cm-30cm ...

[0129] Before feeding, disinfect the zoea larvae, mysid larvae, and juvenile shrimp by soaking them in seawater containing a 10mg / L-20mg / L povidone-iodine solution for 10-20 minutes. After disinfection, rinse them 1-2 times with clean seawater and allow them to recover in oxygenated clean seawater for 1-3 minutes before feeding them to the seahorses.

[0130] Example 6

[0131] This embodiment provides an application of live seahorse feed. Zoea larvae, mysid larvae, and shrimp larvae cultivated in Example 3 are removed from the culture pond, disinfected, and then fed to the seahorses. The corresponding body lengths of the seahorses fed with zoea larvae, mysid larvae, and shrimp larvae at different developmental stages are as follows:

[0132] Feed seahorses with a body length of 3.0cm-3.5cm to stage 1 zoea larvae; 3.5cm-4.0cm to stage 2 zoea larvae; 4.0cm-4.5cm to stage 1 mysid larvae; 4.5cm-5.0cm to stage 2 mysid larvae; 5.0cm-5.5cm to stage 3 mysid larvae; 5.5cm-6.0cm to stage 1 postlarvae; 6.0cm-8.0cm to stage 6mm-10mm to stage 6.0cm-10.0cm to stage 6.0cm-20mm to stage 6.0cm-10.0cm to stage 6.0cm-30mm to stage 20mm-30mm to stage 20mm-30mm to stage 20cm-30cm ...

[0133] Before feeding, disinfect the zoea larvae, mysid larvae, and juvenile shrimp by soaking them in seawater containing a 10mg / L-20mg / L povidone-iodine solution for 10-20 minutes. After disinfection, rinse them 1-2 times with clean seawater and allow them to recover in oxygenated clean seawater for 1-3 minutes before feeding them to the seahorses.

[0134] Furthermore, to illustrate the effects of the present invention, the live biological feed cultivation method and application of this application, as well as conventional frozen biological feed on the market, were used for feeding seahorses with a body length ≥3cm, specifically grouped as follows:

[0135] Group 1: Based on the application of a live seahorse feed provided in Example 4, used to feed seahorses with a body length ≥3cm.

[0136] The second group: Based on the application of a live seahorse feed provided in Example 5, it is used to feed seahorses with a body length of ≥3cm.

[0137] The third group: Based on the application of a live seahorse feed provided in Example 6, it is used to feed seahorses with a body length of ≥3cm.

[0138] Group 4: Seahorses with a body length ≥3cm were fed conventional frozen live food.

[0139] Specifically, after the seahorses grew to a body length of 30mm, they were fed in the above-mentioned grouping method. After 60 days of feeding, the body length of the seahorses in each group was recorded and the average value was taken. At the same time, the incidence of enteritis in each group of seahorses during this feeding period was also recorded.

[0140] Among them, seahorses with swollen and white cloaca, abdominal swelling, and pus discharge upon pressure are identified as having enteritis, while seahorses without the above symptoms are considered healthy seahorses.

[0141] The experimental results are shown in Table 1:

[0142] Table 1: Survival and growth status of seahorses in each group

[0143] Survival rate / % Seahorse body length (cm) Incidence of enteritis / % Group 1 82.75 8.61 1.26 Group 2 86.64 8.72 1.68 Group 3 88.39 8.59 0.77 Group 4 68.41 7.14 4.36

[0144] The experimental results show that, compared with the use of frozen live feed, the use of different live feeds for seahorse farming in this application improves the survival rate and growth rate of seahorses, and also significantly reduces the incidence of seahorse enteritis, thus significantly improving the seahorse farming effect.

[0145] In summary, by utilizing the above-mentioned technical solutions of this invention, the live seahorse feed of this application is rich in nutrients, can be bred and cultivated year-round, is easily consumed by seahorses, and can achieve good breeding results. Based on the feeding habits of seahorses of different sizes, zoea larvae, mysid larvae, and shrimp larvae at different developmental stages are provided, exhibiting good palatability. Furthermore, the use of nutritional enhancers, immune enhancers, and disease control additives avoids nutritional deficiencies and large-scale disease outbreaks during seahorse breeding. The live feed of this application can be fed to newly hatched seahorses after they have been raised to a body length of 3.0 cm using copepod and artichoke larvae, achieving full-process live feed breeding. This avoids the high mortality rate caused by the acclimatization process of using frozen feed, significantly improving the survival rate of seahorse breeding.

[0146] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for cultivating live seahorse food, characterized in that, include: Parent shrimp carrying eggs were placed in an indoor culture pond, and feeding, waste disposal and water exchange were carried out under incubation conditions to obtain the first-stage zoea larvae of Oriental white shrimp. The first-stage zoea larvae were continuously cultured to the first-stage juvenile shrimp; The first-stage larvae were further cultured and fed Artemia nauplii and shrimp starter feed to obtain larvae with a total length of ≤3cm.

2. The method for cultivating live seahorse food according to claim 1, characterized in that, The incubation conditions are as follows: indoor culture tank temperature 15℃-33℃, salinity 5‰-35‰, dissolved oxygen ≥5mg / L, pH 7.5-9.

0.

3. The method for cultivating live seahorse food according to claim 1, characterized in that, The process of placing egg-bearing parent shrimp in an indoor culture tank, and then feeding, waste disposal, and water exchange under incubation conditions to obtain first-stage zoea larvae of the Oriental white shrimp includes: Select egg-bearing Oriental white shrimp broodstock from farmed shrimp, at a ratio of 50 shrimp / m 3 -100 tails / m 3 The density was adjusted and the fish were placed in an indoor aquaculture pond with a water depth of 0.8m-1.2m. Feed the adult shrimp with eggs at 6-8% of their body weight with formulated shrimp feed. Divide the formulated shrimp feed into 4:2:4 ratios and feed them three times a day at 6:00-7:00, 11:00-12:00 and 17:00-18:00 respectively. Before feeding, use a siphon to remove uneaten feed and feces. The water is changed daily at 4 PM, with 1 / 3 to 1 / 2 of the water being replaced. After 15 to 20 days at a temperature between 15℃ and 30℃, the first-stage zoea larvae are obtained.

4. The method for cultivating live seahorse food according to claim 1, characterized in that, The process of continuously culturing stage 1 zoea larvae to stage 1 postlarvae includes: First-stage zoea larvae with a total length of 3.0mm-3.5mm were selected for cultivation and fed with *Phaeodactylum tricornutum* or *Chaetoceros*. After 1-2 days of metamorphosis, they developed into second-stage zoea larvae. The cultivation density was 100,000-150,000 larvae / m². 3 The food density is 300,000-400,000 cells / ml; Second-stage zoea larvae, with a total length of 3.3mm-3.7mm, were selected for cultivation and fed with *Phaeodactylum tricornutum* or *Chaetoceros*, supplemented with *Brachycota foldis*. After 1-2 days of metamorphosis, they developed into first-stage mysid larvae. The cultivation density was 100,000-150,000 larvae / m². 3 The food density was 200,000 cells / ml, and the Brachiopoda foldis density was 15 cells / ml. First-stage mysid larvae with a total length of 3.6mm-3.9mm were selected for rearing and fed with Brachionus rotifer and Artemia nauplii. After 2-3 days of metamorphosis, they developed into second-stage mysid larvae. The rearing density was 100,000-150,000 larvae / m². 3 Brachiopoda foldis was 15 cells / ml, and Artemia nauplii were 5 cells / ml. Second-stage mysid larvae with a total length of 4.0mm-5.0mm were selected for rearing, fed with Artemia nauplii and shrimp microparticle feed, and metamorphosed into third-stage mysid larvae in 2-3 days; the rearing density was 80,000-120,000 larvae / m². 3 The concentration of Artemia nauplii was 5 per ml, and the proportion of microparticle feed for shrimp was 0.06 kg per 10,000 mysid larvae. Third-stage mysid shrimp larvae with a total length of 5.0mm-5.6mm were selected for rearing, fed with Artemia nauplii and shrimp microparticle feed, and metamorphosed into first-stage larvae in 3-4 days; the rearing density was 80,000-120,000 shrimp / m². 3 The concentration of Artemia nauplii was 5 per ml, and the ratio of microparticle feed to shrimp was 0.10 kg per 10,000 mysid larvae.

5. The method for cultivating live seahorse food according to claim 1, characterized in that, The process of continuing to cultivate stage 1 larvae, feeding them Artemia nauplii and shrimp starter feed, to obtain larvae with a total length ≤3cm includes: First-stage larvae with a total length of 5.2-5.8 mm were selected and fed with Artemia nauplii and shrimp starter feed. After 1-2 days, they grew into second-stage larvae; the rearing density was 50,000-100,000 larvae / m². 3 The concentration of Artemia nauplii was 5 per ml, and the ratio of the initial feed for shrimp was 0.10 kg per 10,000 larvae. Second-stage larvae with a total length of 5.7 mm or more were selected and fed Artemia nauplii and shrimp starter feed. After 10-20 days, they entered the juvenile stage; the rearing density was 50,000-100,000 shrimp / m². 3 The concentration of Artemia nauplii was 5 per ml, and the ratio of the initial feed for shrimp was 0.10 kg per 10,000 juvenile shrimp.

6. The method for cultivating live seahorse food according to claim 1, characterized in that, Nutritional enhancers and / or immune enhancers are added to shrimp microparticle feed and / or shrimp starter feed.

7. The method for cultivating live seahorse food according to claim 6, characterized in that, The nutritional enhancer is fish oil, which is added to shrimp microparticle feed and / or shrimp starter feed to provide nutritional fortification for multi-stage zoea larvae, mysid larvae, and postlarvae.

8. The method for cultivating live seahorse food according to claim 7, characterized in that, The immune enhancer is β-glucan, which is added to shrimp microparticle feed and / or shrimp starter feed to enhance the immunity of multi-stage zoea larvae, mysid larvae and postlarvae.

9. An application of a live seahorse feed, used to implement the seahorse live feed cultivation method according to any one of claims 1-8 in seahorses, characterized in that, include: Zoea larvae, mysid larvae, and shrimp juveniles at different developmental stages were removed from the rearing pond, disinfected, and then fed to seahorses. The corresponding body lengths of the seahorses fed to the zoea larvae, mysid larvae, and shrimp juveniles at different developmental stages were as follows: Feed the first-stage zoea larvae with seahorses that are 3.0cm-3.5cm in length; Second-stage zoea larvae were fed seahorses with a body length of 3.5cm-4.0cm; First-stage mysid larvae were fed seahorses with a body length of 4.0cm-4.5cm; Second-stage mysid larvae were fed seahorses with a body length of 4.5cm-5.0cm; Third-stage mysid larvae were fed seahorses with a body length of 5.0cm-5.5cm; First-stage larvae were fed seahorses with a body length of 5.5cm-6.0cm; Juvenile shrimp with a body length of 6mm-10mm were fed seahorses with a body length of 6.0cm-8.0cm; Juvenile shrimp with a body length of 10mm-20mm were fed seahorses with a body length of 8.0cm-10.0cm; Juvenile shrimp with a body length of 20mm-30mm were fed seahorses with a body length of >10cm.

10. The application of a live seahorse feed according to claim 9, characterized in that, Zodiacal larvae, mysid larvae, and shrimp juveniles at different developmental stages were removed from the culture pond, disinfected, and then fed to the seahorse, including: Disinfect zoea larvae, mysid larvae, and juvenile shrimp at different developmental stages by immersing them in seawater containing a 10 mg / L-20 mg / L povidone-iodine solution for 10-20 minutes. After disinfection, rinse them 1-2 times with clean seawater and allow them to recover in oxygenated clean seawater for 1-3 minutes before feeding them to seahorses.

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