Solid fermentation composite plant extract and preparation method and application thereof

Abstract

This invention belongs to the field of cosmetic technology, specifically relating to a solid-state fermented compound plant extract, its preparation method, and its application. The preparation method of this invention includes: pulverizing and sieving dried raw materials of calla lily, andrographis paniculata, nasturtium, snowflake, and snow lotus, respectively; then adding nutrients and adjusting the system's moisture content to 40%-70% and pH value to 5.0-7.0 to obtain a solid-state fermentation substrate; then inoculating with fermentation bacteria for solid-state fermentation; extracting the fermentation products using water or ethanol aqueous solution to obtain solid-state fermented calla lily extract, solid-state fermented andrographis paniculata extract, solid-state fermented nasturtium extract, solid-state fermented snowflake extract, and solid-state fermented snow lotus extract, respectively; mixing and compounding the above extracts to obtain the solid-state fermented compound plant extract. This invention achieves a synergistic effect of multiple efficacy effects of each component through solid-state fermentation and scientific formulation of five plant raw materials with different efficacy characteristics.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a solid fermentation compound plant extract, its preparation method, and its application. Background Technology

[0002] In recent years, with the increasing demand from consumers for natural, safe, and effective cosmetics, the application of plant extracts in the cosmetics field has become increasingly widespread. Traditional methods for preparing plant extracts mainly include physicochemical methods such as solvent extraction, steam distillation, and supercritical fluid extraction. However, these methods have three major problems in cosmetic applications. First, extraction efficiency is limited. Traditional solvent extraction methods retain less than 40% of active ingredients, and the heat treatment process causes a 20%-30% loss of vitamin C. Polyphenolic compounds also degrade by 15%-25% during storage. Second, bioavailability is low. Some active ingredients in plants exist in the form of glycosides, and the hydrophilic nature of glycosyl groups results in poor skin permeability, limiting efficacy. Third, product homogenization is prominent. Traditional extraction methods fail to highlight the unique efficacy of different plant materials, leading to product functional similarities in the market and insufficient competitive advantage through differentiation.

[0003] Fermentation technology, as a biotechnology approach, offers a new direction for upgrading the cosmetic raw material extract industry. Through microbial metabolism, fermentation technology can optimize the chemical composition of plant extracts, improve the bioavailability of active ingredients, and generate new functional compounds. Fermentation technology has several advantages in the application of plant extracts. First, it achieves the reduction of ingredient size, improving penetration efficiency. Fermentation transforms complex macromolecules in plants into simpler, smaller molecular structures, making it easier to penetrate the skin's epidermis and thus increasing ingredient utilization. Second, it enhances biocompatibility. Cosmetic ingredients derived from fermentation have better compatibility with the skin, helping to maintain the balance of the skin's microbiome. Third, it achieves synergistic enhancement of efficacy. Organic acids such as lactic acid and citric acid produced during fermentation can gently regulate the pH value of the skin's surface to a healthy, slightly acidic range, while promoting stratum corneum metabolism, increasing the penetration rate of subsequent active ingredients by approximately 30%. In addition, the fermentation process can also decompose impurities in plant raw materials that may cause skin irritation (such as some plant proteins and tannins), reducing product irritation and improving ingredient stability.

[0004] Solid-state fermentation (SSF) refers to the technology of utilizing microorganisms to carry out metabolic reactions in a substrate with no free water or low moisture content (such as powdered medicinal and edible raw materials or grains). Compared with traditional liquid fermentation, SSF has the following advantages: low cost, no need for complex equipment; high product concentration, easy accumulation of metabolites; low energy consumption, no need for continuous stirring or aeration; and environmental friendliness, with less wastewater discharge and greater sustainability. However, there is currently limited research on the application of SSF technology in extracts from five plants: calla lily, andrographis paniculata, nasturtium, snowflake, and snow lotus. Summary of the Invention

[0005] To address the shortcomings and deficiencies of existing technologies, the primary objective of this invention is to provide a method for preparing a solid-state fermented compound plant extract. This invention is the first to apply solid-state fermentation technology to the preparation of extracts from five plants: calla lily, andrographis paniculata, nasturtium, snowflake, and snow lotus. These five solid-state fermented extracts are scientifically formulated to create a cosmetic composition with multiple effects, including antibacterial, anti-wrinkle, antioxidant, and skin-brightening properties. This overcomes the problems of single-effect plant extracts, low bioavailability, and product homogenization in existing technologies.

[0006] Another object of the present invention is to provide a solid fermentation complex plant extract prepared by the above method.

[0007] Another object of the present invention is to provide the application of the above-mentioned solid fermentation compound plant extract in cosmetics.

[0008] The objective of this invention is achieved through the following technical solution:

[0009] A method for preparing a solid-state fermented compound plant extract includes the following preparation steps:

[0010] (1) Raw material pretreatment: The dried raw materials of calla lily (Zantedeschia aethiopica), andrographis paniculata, nasturtium (Tropaeolum majus), summer snowflake (Leucojumaestivum), and snow lotus (Saussurea involucrata) were crushed and sieved to obtain calla lily raw material powder, andrographis paniculata raw material powder, nasturtium raw material powder, summer snowflake raw material powder and snow lotus raw material powder;

[0011] (2) Preparation of culture medium: Using the raw material powders in step (1) as the main matrix, add nutrients and adjust the moisture content to 40%-70% and the pH value to 5.0-7.0 to obtain solid fermentation substrate;

[0012] (3) Solid fermentation: Fermentation bacteria are inoculated into each solid fermentation substrate in step (2) to carry out solid fermentation and obtain fermentation products;

[0013] (4) Product extraction: Each fermentation product in step (3) is extracted with water or ethanol aqueous solution, filtered and concentrated to obtain solid fermented calla lily extract, solid fermented andrographis paniculata extract, solid fermented nasturtium extract, solid fermented snowflake extract and solid fermented snow lotus extract.

[0014] (5) Synergistic compounding: Mix and compound the extracts in step (4) to obtain solid fermentation compound plant extract.

[0015] Furthermore, the crushing and sieving mentioned in step (1) refers to crushing through a 10-40 mesh sieve.

[0016] Furthermore, the nutrients mentioned in step (2) include at least one of glucose, maltose, sucrose, peptone, yeast extract, beef extract, and inorganic salts.

[0017] Further, the fermentation bacteria mentioned in step (3) include at least one of lactic acid bacteria, yeast, Bacillus, Aspergillus, and Bifidobacterium, and the inoculum size of the fermentation bacteria is 10. 6 -10 8 CFU / g solid fermentation substrate raw material.

[0018] Furthermore, the solid fermentation conditions described in step (3) are: fermentation at 25-37℃ for 3-7 days, during which time the mixture is turned over regularly to maintain suitable humidity and air permeability.

[0019] Furthermore, the volume fraction of ethanol in the aqueous ethanol solution described in step (4) is 50%–90%.

[0020] Furthermore, the extraction conditions for the product described in step (4) are: extraction at 40-80℃ for 2-4 hours.

[0021] A solid-state fermented compound plant extract was prepared by the above method.

[0022] The application of the above-mentioned solid-state fermented compound plant extracts in cosmetic preparation.

[0023] Furthermore, the cosmetic is a serum, cream, lotion, gel, or mask, and the mass concentration of the solid fermented compound plant extract in the cosmetic is: 0.1%-5% solid fermented calla lily extract, 0.1%-5% solid fermented andrographis paniculata extract, 0.1%-5% solid fermented nasturtium extract, 0.01%-3% solid fermented snowflake extract, and 0.1%-5% solid fermented snow lotus extract.

[0024] More preferably, the mass concentration of the solid fermented compound plant extract in the cosmetic is as follows: 0.5%-3% solid fermented calla lily extract, 0.5%-3% solid fermented andrographis paniculata extract, 0.5%-3% solid fermented nasturtium extract, 0.05%-2% solid fermented snowflake extract, and 0.5%-3% solid fermented snow lotus extract.

[0025] Most preferably, the mass concentration of the solid fermented compound plant extract in the cosmetic is: 1%-2% solid fermented calla lily extract, 1%-2% solid fermented andrographis paniculata extract, 1%-2% solid fermented nasturtium extract, 0.1%-1% solid fermented snowflake extract, and 1%-2% solid fermented snow lotus extract.

[0026] This invention achieves a synergistic effect of multiple benefits by scientifically combining five plant extracts with different efficacy properties through solid-state fermentation:

[0027] 1. Synergistic antioxidant effect: The combined antioxidant activity of the five solid fermented plant extracts is significantly higher than that of the single ingredients, with a DPPH free radical scavenging rate of over 75%, which can effectively remove free radicals in the skin and delay skin aging.

[0028] 2. Broad-spectrum antibacterial effect: Solid-state fermented compound plant extract has a good inhibitory effect on Gram-positive bacteria, Gram-negative bacteria and fungi. The total number of bacteria on the skin surface is reduced by more than 65% and the total number of fungi is reduced by more than 58%, which can effectively prevent and treat skin infections.

[0029] 3. Multiple anti-wrinkle mechanisms: Through multiple mechanisms such as inhibiting elastase activity (inhibition rate of about 40%-50%), promoting collagen synthesis, and enhancing skin elasticity, it significantly improves skin wrinkles, reduces wrinkle depth by more than 15%, and improves skin elasticity by more than 13%.

[0030] 4. Comprehensive skin brightening effect: By inhibiting tyrosinase activity (inhibition rate of over 65%), reducing melanin synthesis, and promoting skin metabolism, it achieves whitening and spot-fading effects, increasing skin brightness by over 12% and improving skin tone evenness by over 15%.

[0031] 5. Safe and gentle: Human trials and safety evaluations show that the product is gentle, non-irritating, and non-allergenic, making it suitable for all skin types.

[0032] 6. Natural and environmentally friendly: Made with natural plant extracts, meeting consumers' demand for natural cosmetics and having a promising market prospect. Detailed Implementation

[0033] The present invention will be further described in detail below with reference to embodiments, but the implementation of the present invention is not limited thereto.

[0034] The fermentation bacteria strains used in the following examples were all commercially available:

[0035] Lactobacillus plantarum: CGMCC No. 14565, China General Microbiological Culture Collection Center.

[0036] Saccharomyces cerevisiae: CGMCC No.22020, China General Microbiological Culture Collection Center.

[0037] Bacillus subtilis: CICC 10732, China Industrial Microbial Culture Collection Center.

[0038] Lactobacillus acidophilus: CICC 6074, China Industrial Microbial Culture Collection Center.

[0039] Aspergillus niger: CICC 2377, China Industrial Microbial Culture Collection Center.

[0040] Aspergillus oryzae: CICC 2078, China Industrial Microbial Culture Collection Center.

[0041] Lactobacillus bulgaricus: CICC 6047, China Industrial Microbial Culture Collection Center.

[0042] Bifidobacterium bifidum: CICC 6071, China Industrial Microbial Culture Collection Center.

[0043] Lactobacillus casei: CICC 20284, China Industrial Microbial Culture Collection Center.

[0044] Example 1

[0045] Preparation of solid-state fermented calla lily extract (I):

[0046] 1. Take 100g of dried calla lily flowers / stems and grind them to 20 mesh.

[0047] 2. Add 5g of glucose and 3g of peptone, and mix well.

[0048] 3. Adjust the moisture content to 60% and the pH value to 6.0.

[0049] 4. Inoculate with Lactobacillus plantarum 10 7 CFU / g raw material.

[0050] 5. Ferment at 30℃ for 5 days. Stir regularly during this period to maintain suitable humidity and air permeability.

[0051] 6. After fermentation, extract with 200 mL of 70% ethanol at 60°C for 3 hours.

[0052] 7. Filter and concentrate to obtain 15.2g of solid-state fermented calla lily extract (I).

[0053] Example 2

[0054] Preparation of solid-state fermented calla lily extract (II):

[0055] 1. Take 100g of dried calla lily flowers / stems and grind them into 30 mesh.

[0056] 2. Add 5g of yeast extract and 2g of potassium dihydrogen phosphate, and mix well.

[0057] 3. Adjust the moisture content to 55% and the pH value to 5.5.

[0058] 4. Inoculate with 10g of Saccharomyces cerevisiae 7 CFU / g raw material.

[0059] 5. Ferment at 28℃ for 6 days. Stir regularly during this period to maintain suitable humidity and air permeability.

[0060] 6. After fermentation, extract with 200mL of water at 80℃ for 2 hours.

[0061] 7. Filter and concentrate to obtain 12.8g of solid-state fermented calla lily extract.

[0062] Example 3

[0063] Preparation of solid-state fermented Andrographis paniculata extract (I):

[0064] 1. Take 100g of dried Andrographis paniculata leaves and grind them to 15 mesh.

[0065] 2. Add 8g of glucose and 3g of beef extract, and mix well.

[0066] 3. Adjust the moisture content to 65% and the pH value to 6.5.

[0067] 4. Inoculate with Bacillus subtilis 108 CFU / g raw material.

[0068] 5. Ferment at 35℃ for 4 days. Stir regularly during this period to maintain suitable humidity and air permeability.

[0069] 6. After fermentation, extract with 200 mL of 80% ethanol at 70°C for 3 hours.

[0070] 7. Filter and concentrate to obtain 18.5g of solid fermented Andrographis paniculata extract (I).

[0071] Example 4

[0072] Preparation of solid-state fermented Andrographis paniculata extract (II):

[0073] 1. Take 100g of dried Andrographis paniculata leaves and grind them to 25 mesh.

[0074] 2. Add 5g of peptone and 3g of yeast extract, and mix well.

[0075] 3. Adjust the moisture content to 50% and the pH value to 7.0.

[0076] 4. Inoculate with 10g of lactic acid bacteria (Lactobacillus acidophilus). 7 CFU / g raw material.

[0077] 5. Ferment at 25℃ for 7 days. Stir regularly during this period to maintain suitable humidity and air permeability.

[0078] 6. After fermentation, extract with 200 mL of water at 60°C for 4 hours.

[0079] 7. Filter and concentrate to obtain 14.3g of solid fermented Andrographis paniculata extract (II).

[0080] Example 5

[0081] Preparation of solid-state fermented nasturtium extract (I):

[0082] 1. Take 100g of dried nasturtium whole herb and grind it to 20 mesh.

[0083] 2. Add 6g of glucose and 2g of dipotassium hydrogen phosphate, and mix well.

[0084] 3. Adjust the moisture content to 60% and the pH value to 6.0.

[0085] 4. Inoculate with Aspergillus niger 10 7 CFU / g raw material.

[0086] 5. Ferment at 30℃ for 5 days. Stir regularly during this period to maintain suitable humidity and air permeability.

[0087] 6. After fermentation, extract with 200 mL of 75% ethanol at 65°C for 3 hours.

[0088] 7. Filter and concentrate to obtain 16.7g of solid-state fermented nasturtium extract (I).

[0089] Example 6

[0090] Preparation of solid-state fermented nasturtium extract (II):

[0091] 1. Take 100g of dried nasturtium whole herb and grind it to 30 mesh.

[0092] 2. Add 8g of sucrose and 4g of yeast extract, and mix well.

[0093] 3. Adjust the moisture content to 55% and the pH value to 5.5.

[0094] 4. Inoculate raw material with 10^7 CFU / g of Aspergillus oryzae.

[0095] 5. Ferment at 28℃ for 6 days. Stir regularly during this period to maintain suitable humidity and air permeability.

[0096] 6. After fermentation, extract with 200 mL of water at 70°C for 2.5 hours.

[0097] 7. Filter and concentrate to obtain 13.9g of solid-state fermented nasturtium extract (II).

[0098] Example 7

[0099] Preparation of solid-state fermented summer snowflake extract (I):

[0100] 1. Take 100g of dried summer snowflake bulbs and grind them to 15 mesh.

[0101] 2. Add 10g of glucose and 5g of peptone, and mix well.

[0102] 3. Adjust the moisture content to 70% and the pH value to 6.8.

[0103] 4. Inoculate with 10g of lactic acid bacteria (Lactobacillus bulgaricus) 8 CFU / g raw material.

[0104] 5. Ferment at 37℃ for 3 days. Stir regularly during this period to maintain suitable humidity and air permeability.

[0105] 6. After fermentation, extract with 200 mL of 85% ethanol at 75°C for 2 hours.

[0106] 7. Filter and concentrate to obtain 11.2g of solid-state fermented snowflake extract (I).

[0107] Example 8

[0108] Preparation of solid-state fermented summer snowflake extract (II):

[0109] 1. Take 100g of dried summer snowflake bulbs and grind them to 25 mesh.

[0110] 2. Add 8g of maltose and 3g of yeast extract, and mix well.

[0111] 3. Adjust the moisture content to 50% and the pH value to 7.2.

[0112] 4. Inoculate with Bifidobacterium bifidum 10 7 CFU / g raw material.

[0113] 5. Ferment at 25℃ for 7 days. Stir regularly during this period to maintain suitable humidity and air permeability.

[0114] 6. After fermentation, extract with 200mL of water at 80℃ for 3 hours.

[0115] 7. Filter and concentrate to obtain 9.8g of solid-state fermented snowflake extract (II).

[0116] Example 9

[0117] Preparation of solid-state fermented snow lotus extract (I):

[0118] 1. Take 100g of dried snow lotus and grind it to 20 mesh.

[0119] 2. Add 7g of glucose and 4g of peptone, and mix well.

[0120] 3. Adjust the moisture content to 65% and the pH value to 6.5.

[0121] 4. Inoculate with 10g of Saccharomyces cerevisiae 7 CFU / g raw material.

[0122] 5. Ferment at 30℃ for 5 days. Stir regularly during this period to maintain suitable humidity and air permeability.

[0123] 6. After fermentation, extract with 200 mL of 80% ethanol at 70°C for 3 hours.

[0124] 7. Filter and concentrate to obtain 17.6g of solid fermented snow lotus extract (I).

[0125] Example 10

[0126] Preparation of solid-state fermented snow lotus extract (II):

[0127] 1. Take 100g of dried snow lotus and grind it into 30 mesh.

[0128] 2. Add 6g of sucrose and 3g of yeast extract, and mix well.

[0129] 3. Adjust the moisture content to 55% and the pH value to 6.0.

[0130] 4. Inoculate with 10g of lactic acid bacteria (Lactobacillus casei) 7 CFU / g raw material.

[0131] 5. Ferment at 28℃ for 6 days. Stir regularly during this period to maintain suitable humidity and air permeability.

[0132] 6. After fermentation, extract with 200 mL of water at 65°C for 3.5 hours.

[0133] 7. Filter and concentrate to obtain 15.1g of solid fermented snow lotus extract (II).

[0134] Example 11

[0135] The application of a solid-state fermented compound plant extract in the preparation of a serum, wherein the serum formulation (mass percentage) is as follows:

[0136] Solid-state fermented calla lily extract (I): 1.5%;

[0137] Solid-state fermented Andrographis paniculata extract (I): 1.5%;

[0138] Solid-state fermented nasturtium extract (I): 1.0%;

[0139] Solid-state fermented snowflake extract (I): 0.3%;

[0140] Solid-state fermented snow lotus extract (I): 1.5%;

[0141] Sodium hyaluronate: 0.2%;

[0142] Glycerin: 5.0%;

[0143] Butanediol: 3.0%;

[0144] 1,2-Hexanediol: 0.5%;

[0145] p-Hydroxyacetophenone: 0.3%;

[0146] Water: Balance.

[0147] Preparation method:

[0148] 1. Disperse sodium hyaluronate in some water and stir until completely dissolved.

[0149] 2. Add glycerin, butylene glycol, 1,2-hexanediol, and p-hydroxyacetophenone in sequence, and stir until well mixed.

[0150] 3. Add the solid fermented plant extracts and mix thoroughly.

[0151] 4. Add water to make up the remaining amount and adjust the pH value to 6.0±0.2.

[0152] 5. Filter, fill, and you have the product.

[0153] Example 12

[0154] The application of a solid-state fermented compound plant extract in the preparation of a face cream, wherein the face cream formula (by weight percentage) is as follows:

[0155] Solid-state fermented calla lily extract (II): 2.0%;

[0156] Solid-state fermented Andrographis paniculata extract (II): 2.0%;

[0157] Solid-state fermented nasturtium extract (II): 1.5%;

[0158] Solid-state fermented snowflake extract (II): 0.5%;

[0159] Solid-state fermented snow lotus extract (II): 2.0%;

[0160] Stearic acid: 3.0%;

[0161] Cetyl alcohol: 2.0%;

[0162] Glyceryl stearate: 1.5%;

[0163] Montanov L: 2.0%;

[0164] Glycerin: 5.0%;

[0165] Sodium hyaluronate: 0.1%;

[0166] Phenoxyethanol: 0.8%;

[0167] Water: Balance.

[0168] Preparation method:

[0169] 1. Heat the aqueous phase components (sodium hyaluronate, glycerin, and water) to 80°C and stir until completely dissolved.

[0170] 2. Heat the oil phase components (stearic acid, cetyl alcohol, glyceryl stearate, Montanov L) to 80°C and stir until completely dissolved.

[0171] 3. Under stirring conditions, slowly add the oil phase to the aqueous phase and homogenize for 5 minutes.

[0172] 4. Cool to 45°C, then add the solid fermented plant extracts and phenoxyethanol.

[0173] 5. Continue stirring until room temperature, and the product is ready.

[0174] Example 13

[0175] The application of a solid-state fermented compound plant extract in gel preparation, wherein the gel formulation (mass percentage) is as follows:

[0176] Solid-state fermented calla lily extract (I): 1.0%;

[0177] Solid-state fermented Andrographis paniculata extract (I): 1.0%;

[0178] Solid-state fermented nasturtium extract (II): 0.8%;

[0179] Solid-state fermented snowflake extract (II): 0.2%;

[0180] Solid-state fermented snow lotus extract (II): 1.0%;

[0181] Carbomer 940: 0.3%;

[0182] Triethanolamine: 0.3%;

[0183] Glycerin: 8.0%;

[0184] 1,3-Propane glycol: 5.0%;

[0185] Allantoin: 0.2%;

[0186] Phenoxyethanol: 0.8%;

[0187] Water: Balance.

[0188] Preparation method:

[0189] 1. Disperse Carbomer 940 in water and soak overnight to allow it to fully swell.

[0190] 2. Add glycerin, 1,3-propanediol, and allantoin, and stir well.

[0191] 3. Adjust the pH to 6.5 ± 0.2 using triethanolamine.

[0192] 4. Add the solid fermented plant extracts and phenoxyethanol, and stir well.

[0193] 5. Let stand to defoam, then fill to obtain the product.

[0194] Comparative Example 1

[0195] An essence, compared to Example 11, uses 5.8% of a single solid-fermented calla lily extract (I) instead of a solid-fermented compound plant extract.

[0196] Comparative Example 2

[0197] An essence, compared to Example 11, uses 5.8% of a single solid-fermented Andrographis paniculata extract (I) instead of a solid-fermented compound plant extract.

[0198] Comparative Example 3

[0199] An essence, compared to Example 11, uses 5.8% of a single solid-fermented nasturtium extract (I) instead of a solid-fermented compound plant extract.

[0200] Comparative Example 4

[0201] An essence, compared to Example 11, uses 5.8% of a single solid-fermented snowdrop extract (I) instead of a solid-fermented compound plant extract.

[0202] Comparative Example 5

[0203] An essence, compared to Example 11, uses 5.8% of a single solid-fermented snow lotus extract (I) instead of a solid-fermented compound plant extract.

[0204] Comparative Example 6

[0205] An essence, compared to Example 11, uses the same amount of unfermented calla lily extract, andrographis paniculata extract, nasturtium extract, snowflake extract, and snow lotus extract to replace solid-fermented calla lily extract, andrographis paniculata extract, and solid-fermented nasturtium extract, and solid-fermented snowflake extract, respectively.

[0206] The preparation methods for the unfermented calla lily extract, andrographis paniculata extract, nasturtium extract, snowflake extract, and snow lotus extract are as follows:

[0207] Calla lily extract: Take 100g of dried calla lily flowers / stems, grind to 20 mesh, and extract with 200mL of 70% ethanol at 60℃ for 3 hours. Filter and concentrate to obtain 12.4g of calla lily extract.

[0208] Andrographis paniculata extract: Take 100g of dried Andrographis paniculata leaves, grind them to 15 mesh, and extract with 200mL of 80% ethanol at 70℃ for 3 hours. Filter and concentrate to obtain 13.8g of Andrographis paniculata extract.

[0209] Nasturtium extract: Take 100g of dried nasturtium whole herb, grind it to 20 mesh, and extract it with 200mL of 75% ethanol at 65℃ for 3 hours. Filter and concentrate to obtain 12.0g of nasturtium extract.

[0210] Summer Snowflake Extract: Take 100g of dried summer snowflake bulbs, pulverize to 15 mesh, and extract with 200mL of 85% ethanol at 75℃ for 2 hours. Filter and concentrate to obtain 9.5g of summer snowflake extract.

[0211] Snow lotus extract: Take 100g of dried snow lotus, grind it to 20 mesh, and extract it with 200mL of 80% ethanol at 70℃ for 3 hours. Filter and concentrate to obtain 14.7g of solid-state fermented snow lotus extract.

[0212] Efficacy verification experiment

[0213] 1. In vitro antioxidant activity assay

[0214] The antioxidant activity of the compositions was evaluated using a DPPH free radical scavenging assay. Compositions of Examples 11-13 and Comparative Examples 1-6 at a concentration of 1 mg / mL were mixed with DPPH solution and reacted at room temperature in the dark for 30 minutes before absorbance was measured. The DPPH free radical scavenging rate of the compositions was calculated, and the results are shown in Table 1 below.

[0215] Table 1 Results of antioxidant activity assay

[0216]

[0217] As can be seen from the results in Table 1, the antioxidant activity of the solid-state fermented compound plant extract of the present invention is significantly higher than that of the single solid-state fermented plant extract, and also significantly higher than that of the unfermented compound plant extract.

[0218] 2. In vitro antibacterial activity assay

[0219] The antibacterial activity of the compositions of Examples 11-13 and Comparative Examples 1-6 against common skin pathogens was determined by the agar diffusion method, and the diameter of the inhibition zone was compared. The results are shown in Table 2 below.

[0220] Table 2 Results of antibacterial activity assay

[0221]

[0222] As can be seen from the results in Table 2, the antibacterial activity of the solid-state fermented compound plant extract of the present invention is significantly higher than that of the single solid-state fermented plant extract, and also significantly higher than that of the unfermented compound plant extract.

[0223] 3. In vitro whitening efficacy determination

[0224] The inhibitory activity of the compositions of Examples 11-13 and Comparative Examples 1-6 at a concentration of 0.5 mg / mL against tyrosinase was determined using the L-DOPA method. The results are shown in Table 3 below.

[0225] Table 3 Results of Whitening Efficacy Test

[0226]

[0227] As can be seen from the results in Table 3, the whitening effect of the solid-state fermented compound plant extract of the present invention is significantly higher than that of the single solid-state fermented plant extract, and also significantly higher than that of the unfermented compound plant extract.

[0228] 4. In vitro anti-wrinkle efficacy test

[0229] The anti-wrinkle efficacy of the compositions of Examples 11-13 and Comparative Examples 1-6 at different concentrations (100 μg / mL, 250 μg / mL) was evaluated using an elastase inhibition assay. The results are shown in Table 4 below.

[0230] Table 4 Results of anti-wrinkle efficacy test

[0231]

[0232] As can be seen from the results in Table 4, the anti-wrinkle effect of the solid-state fermented compound plant extract of the present invention is significantly higher than that of the single solid-state fermented plant extract, and also significantly higher than that of the unfermented compound plant extract.

[0233] 5. Human efficacy trials

[0234] One hundred healthy subjects (aged 25-50 years) were randomly divided into 10 groups of 10 each. The experimental groups used the serum compositions described in Examples 11-13 and Comparative Examples 1-6, respectively, while the control group used a blank matrix without active ingredients (plant extracts). The serums were used twice daily for 8 weeks. The following efficacy evaluations were conducted:

[0235] (1) Moisturizing effect: The percentage increase in skin moisture content in the experimental group compared to the control group.

[0236] (2) Anti-wrinkle effect: percentage reduction in wrinkle depth and percentage increase in skin elasticity in the experimental group compared to the control group.

[0237] (3) Skin brightening effect: percentage increase in skin brightness (L* value) and percentage improvement in skin tone uniformity in the experimental group compared to the control group.

[0238] (4) Antibacterial effect: The percentage reduction in the total number of bacteria on the skin surface of the experimental group relative to the control group and the percentage reduction in the total number of fungi.

[0239] The corresponding test results are shown in Table 5 below.

[0240] Table 5 Results of Human Efficacy Trials

[0241]

[0242] As shown in Table 5, the moisturizing, anti-wrinkle, skin-brightening, and antibacterial effects of the solid-state fermented compound plant extract of this invention are significantly higher than those of single solid-state fermented plant extracts, and also significantly higher than those of unfermented compound plant extracts. This is basically consistent with the results of in vitro efficacy verification.

[0243] 6. Skin irritation test

[0244] Human patch testing was used to evaluate the safety of the product. The compositions of Examples 11-13 of this invention were applied to the backs of 30 healthy subjects, and skin reactions were observed after 24 hours. The results showed that no adverse reactions such as erythema or edema occurred in any of the subjects, indicating that the product is mild and non-irritating.

[0245] 7. Skin sensitization test

[0246] The sensitization properties of the compositions of Examples 11-13 of this invention were evaluated using a repeated use test. After 21 days of continuous use of the compositions of this invention, no allergic reactions were observed in any of the subjects, indicating that the products are safe.

[0247] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. A method for preparing a solid-state fermented compound plant extract, characterized in that, The preparation steps include the following: (1) Raw material pretreatment: The dried raw materials of calla lily, andrographis paniculata, nasturtium, summer snowflake lotus and snow lotus are crushed and sieved to obtain calla lily raw material powder, andrographis paniculata raw material powder, nasturtium raw material powder, summer snowflake lotus raw material powder and snow lotus raw material powder. (2) Preparation of culture medium: Using the raw material powders in step (1) as the main matrix, add nutrients and adjust the moisture content to 40%-70% and the pH value to 5.0-7.0 to obtain solid fermentation substrate; (3) Solid fermentation: Fermentation bacteria are inoculated into each solid fermentation substrate in step (2) to carry out solid fermentation and obtain fermentation products; (4) Product extraction: Each fermentation product in step (3) is extracted with water or ethanol aqueous solution, filtered and concentrated to obtain solid fermented calla lily extract, solid fermented andrographis paniculata extract, solid fermented nasturtium extract, solid fermented snowflake extract and solid fermented snow lotus extract. (5) Synergistic compounding: Mix and compound the extracts in step (4) to obtain solid fermentation compound plant extract.

2. The method for preparing a solid-state fermented compound plant extract according to claim 1, characterized in that: The crushing and sieving mentioned in step (1) refers to crushing through a 10-40 mesh sieve.

3. The method for preparing a solid-state fermented compound plant extract according to claim 1, characterized in that: The nutrients mentioned in step (2) include at least one of glucose, maltose, sucrose, peptone, yeast extract, beef extract, and inorganic salts.

4. The method for preparing a solid-state fermented compound plant extract according to claim 1, characterized in that: The fermentation bacteria mentioned in step (3) include at least one of lactic acid bacteria, yeast, Bacillus, Aspergillus, and Bifidobacterium, and the inoculum size is 10. 6 -10 8 CFU / g solid fermentation substrate raw material.

5. The method for preparing a solid-state fermented compound plant extract according to claim 1, characterized in that: The solid fermentation conditions described in step (3) are: fermentation at 25-37℃ for 3-7 days, with regular stirring during the period to maintain suitable humidity and air permeability.

6. The method for preparing a solid-state fermented compound plant extract according to claim 1, characterized in that: The volume fraction of ethanol in the aqueous ethanol solution in step (4) is 50%-90%, and the extraction conditions for the product are: extraction at 40-80℃ for 2-4 hours.

7. A solid-state fermented compound plant extract, characterized in that: It is prepared by the method described in any one of claims 1-6.

8. The application of the solid-state fermented compound plant extract according to claim 7 in cosmetic preparation, characterized in that: The cosmetic is a serum, cream, lotion, gel, or mask. The mass concentration of the solid fermented compound plant extract in the cosmetic is as follows: 0.1%-5% solid fermented calla lily extract, 0.1%-5% solid fermented andrographis paniculata extract, 0.1%-5% solid fermented nasturtium extract, 0.01%-3% solid fermented snowflake extract, and 0.1%-5% solid fermented snow lotus extract.

9. The application of a solid-state fermented compound plant extract according to claim 8 in cosmetic preparation, characterized in that: The mass concentrations of the solid-state fermented compound plant extracts in cosmetics are as follows: 0.5%–3% solid-state fermented calla lily extract, 0.5%–3% solid-state fermented andrographis paniculata extract, 0.5%–3% solid-state fermented nasturtium extract, 0.05%–2% solid-state fermented snowflake extract, and 0.5%–3% solid-state fermented snow lotus extract.

10. The application of a solid-state fermented compound plant extract according to claim 8 in the preparation of cosmetics, characterized in that: The mass concentration of the solid fermented compound plant extracts in cosmetics is as follows: 1%-2% solid fermented calla lily extract, 1%-2% solid fermented andrographis paniculata extract, 1%-2% solid fermented nasturtium extract, 0.1%-1% solid fermented snowflake extract, and 1%-2% solid fermented snow lotus extract.

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