A scalp anti-aging composition with ultraviolet protection function and application thereof

Abstract

The present application belongs to the technical field of cosmetics, and particularly relates to a scalp anti-aging composition with ultraviolet protection function and application thereof. The present application fully plays the synergistic effect of each component in antioxidant, anti-inflammatory and barrier repair by scientifically compounding phytolacca americana extract, solanum incanum root extract, acorus calamus root extract and camellia chrysantha flower extract. The composition can significantly absorb ultraviolet rays, at the same time, the composition can significantly down-regulate the expression of ultraviolet-induced inflammatory factors, relieve scalp inflammatory reaction, and protect hair follicle health. In addition, the composition can also improve skin elasticity and tightness, improve the relaxed state of the scalp, and delay the aging process of the scalp. The preparation method of the composition of the present application is simple, each component is natural, and the safety is high, which is suitable for preparing scalp care products with multiple functions of anti-photoaging, anti-inflammatory, tightening and the like, and has good market application prospect.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a scalp anti-aging composition with ultraviolet protection function and its application. Background Technology

[0002] Ultraviolet (UV) radiation is the primary cause of photoaging of the skin. Its aging-promoting mechanism spans the entire process of cellular structural damage, molecular signaling disorders, and metabolic imbalances, accounting for more than 80% of the causes of skin aging. UV radiation is divided into UVA (long-wave, accounting for 95% of sunlight's ultraviolet radiation) and UVB (medium-wave). UVA has strong penetrating power and can reach the dermis, triggering oxidative stress by producing reactive oxygen species (ROS), damaging collagen and elastic fibers in the dermis, leading to skin laxity and wrinkles. At the same time, ROS damages cellular DNA, inducing gene mutations and accelerating cell aging or apoptosis. UVB mainly acts on the epidermis, directly damaging the DNA pyrimidine dimer structure, inhibiting fibroblast proliferation and collagen synthesis, and activating inflammatory pathways such as NF-κB, releasing pro-inflammatory factors such as TNF-α and IL-6, triggering chronic inflammation, and further aggravating collagen degradation and skin barrier damage. In addition, ultraviolet rays can inhibit the skin's antioxidant system, reduce the activity of antioxidants such as superoxide dismutase (SOD) and glutathione, and form a vicious cycle of "oxidative damage-inflammatory response-collagen loss", which ultimately leads to aging characteristics such as wrinkles, age spots, and roughness.

[0003] Therefore, anti-photoaging is an important branch of anti-aging technology research. In the cosmetics market, anti-photoaging and sunscreen products are very popular with consumers, and their market share continues to rise, indicating that this type of product market has stable growth potential and will continue to maintain a stable development trend in the coming years. Compared to chemically synthesized and biosynthesized products, naturally extracted ingredients are generally safer and more environmentally friendly, and are more likely to be favored by consumers. Given these characteristics, there is still a need to develop anti-aging scalp products specifically for photoaging. Summary of the Invention

[0004] The purpose of this section is to outline some aspects of the embodiments of the present invention and to briefly describe some preferred embodiments.

[0005] As one aspect of the present invention, the present invention provides a scalp anti-aging composition with ultraviolet protection function, characterized in that the scalp anti-aging composition comprises Kochia scoparia fruit extract, Solanum nigrum root extract, Acorus calamus root extract and Camellia chrysantha flower extract, wherein the weight ratio of Kochia scoparia fruit extract, Solanum nigrum root extract, Acorus calamus root extract and Camellia chrysantha flower extract is (10-25):(7-56):(15-35):(20-50).

[0006] As a preferred embodiment of the scalp anti-aging composition with ultraviolet protection function described in this invention, the weight ratio of the Kochia scoparia fruit extract, Solanum nigrum root extract, Acorus calamus root extract and Camellia chrysantha flower extract is (20-25):(21-56):(25-35):(25-50).

[0007] As a preferred embodiment of the scalp anti-aging composition with UV protection function described in this invention, the preparation method of the nightshade root extract includes: pulverizing and sieving dried nightshade root, adding pure water at a material-to-liquid ratio of 1:(20-30), ultrasonically extracting at 45-60 ℃ for 2-8 h, centrifuging and filtering to obtain the filtrate.

[0008] As a preferred embodiment of the scalp anti-aging composition with ultraviolet protection function described in this invention, the preparation method of the Camellia chrysantha extract includes crushing Camellia chrysantha flowers, adding an extraction solvent, refluxing at 70-80 °C, filtering and collecting the extract, and concentrating; the solvent includes an ethanol solution with a mass concentration of 60-70%.

[0009] As a preferred embodiment of the scalp anti-aging composition with UV protection function described in this invention, the extraction solvent further includes 5-15% by mass of ethoxydiethylene glycol; the material-to-liquid ratio of the extraction solvent is 1:(10-20); and the reflux extraction time is 1-4 h.

[0010] As a preferred embodiment of the scalp anti-aging composition with ultraviolet protection function described in this invention, the extraction solvent is a 65% aqueous ethanol solution containing 10% ethoxydiethylene glycol.

[0011] As a preferred embodiment of the scalp anti-aging composition with UV protection function according to the present invention, the composition further includes at least one of oils, peptides, or collagen; the oils are selected from macadamia nut oil, wheat germ oil, or rice bran oil; the peptides are selected from one or more of oligopeptide-1, acetyl tetrapeptide-3, tripeptide-1, or hexapeptide-9; and the collagen is selected from recombinant type 3 collagen or recombinant type 17 collagen.

[0012] As a preferred embodiment of the scalp anti-aging composition with ultraviolet protection function described in this invention, the composition further includes glycolipids, including lactone-type sophorolipids.

[0013] The present invention also provides the use of the scalp anti-aging composition with ultraviolet protection function in the preparation of cosmetics with scalp anti-aging, ultraviolet protection or anti-inflammatory effects.

[0014] The cosmetics include at least one of the following: hair pre-shampoo, shampoo, hair oil, conditioner, hair mask, hair serum, or hair oil.

[0015] The beneficial effects of this invention are as follows: This invention scientifically combines extracts of Kochia scoparia fruit, Solanum nigrum root, Acorus calamus root, and Camellia chrysantha flower to fully leverage the synergistic effects of each component in anti-oxidation, anti-inflammation, and barrier repair. This composition can significantly absorb ultraviolet light, effectively blocking UVA and UVB damage to the scalp and reducing photoaging factors at the source. Simultaneously, the composition can significantly downregulate the expression of UV-induced inflammatory factors, alleviating scalp inflammation and protecting hair follicle health. Furthermore, this composition can improve skin elasticity and firmness, improve scalp laxity, and delay the aging process of the scalp. By introducing auxiliary ingredients such as oils, peptides, collagen, or glycolipids, the repair and nourishing effects of the composition can be further enhanced. The preparation method of the composition of this invention is simple, the components are all naturally derived, and it has high safety. It is suitable for preparing scalp care products with multiple effects such as anti-photoaging, anti-inflammation, and firming, and has good market application prospects. Detailed Implementation

[0016] To make the above-mentioned objectives, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to specific examples.

[0017] Kochia scoparia fruit is a plant belonging to the genus Kochia of the family Chenopodiaceae. It has pharmacological effects such as lowering blood sugar, anti-inflammation, anti-allergy, anti-itch, anti-pathogenic microorganism, anti-oxidation, and anti-aging.

[0018] Solanum nigrum, a plant belonging to the Solanaceae family and the Solanum genus, can enhance the activity of endogenous antioxidant enzymes such as superoxide dismutase and glutathione, effectively scavenging free radicals, reducing oxidative damage caused by ultraviolet rays and stress, exerting anti-aging effects, anti-inflammatory properties, regulating scalp immunity, protecting the scalp barrier, and reducing inflammation damage to hair follicles.

[0019] Sweet flag root is mostly the dried rhizome of the sweet flag plant (Acorus calamus) in the Araceae family. The extract contains volatile oils (β-asarone, α-asarone, caryophyllene), flavonoids, tannins, amino acids, sugars and other components.

[0020] Golden camellia is a plant belonging to the Camellia genus of the Theaceae family. Its flower extract contains flavonoids, polysaccharides, saponins, tea polyphenols, amino acids, vitamins, and trace elements such as selenium and zinc.

[0021] Kochia scoparia fruit extract: purchased from Huzhou Jiamei Biochemical Products Co., Ltd., "Kochia scoparia fruit extract".

[0022] Solanum nigrum root extract: The dried Solanum nigrum root was crushed and passed through a 100-mesh sieve. Pure water was added at a material-to-liquid ratio of 1:25, and the extract was ultrasonically extracted at 50°C for 5 hours. After extraction, the extract was centrifuged at 8000 rpm and then filtered through a 1µm and 220nm filter membrane to obtain a clear filtrate. Butanediol with a mass concentration of 3% and p-hydroxyacetophenone with a mass concentration of 0.5% were added to obtain Solanum nigrum root extract.

[0023] Sweet flag root extract: purchased from Zhongzhi (Shandong) Biotechnology Co., Ltd., "CMZZ-Sweet flag root extract".

[0024] Preparation methods of Camellia petelotii (Merr.) Sealy flower extract in Examples 1-2 and Comparative Examples 1-3: Camellia petelotii flowers were collected, dried, ground, and sieved through a 100-mesh sieve to obtain Camellia petelotii flower powder. A 65% (v / v) ethanol aqueous solution was added to the Camellia petelotii flower powder at a material-to-liquid ratio of 1g:14mL. The mixture was stirred and extracted under reflux at 80°C for 3 hours. After extraction, the mixture was coarsely filtered, then filtered through a 220nm filter membrane, and the filtrate was collected. The filtrate was concentrated at 50°C and spray-dried in a spray drying tower to obtain Camellia petelotii flower extract.

[0025] Example 3: Preparation method of Camellia chrysantha flower extract: Camellia chrysantha flowers were collected, dried, ground and pulverized, and passed through a 100-mesh sieve to obtain Camellia chrysantha flower powder; a solvent was added to the Camellia chrysantha flower powder at a material-to-liquid mass ratio of 1g:14mL, wherein the solvent was an aqueous solution containing 10% ethoxydiethylene glycol and 65% ethanol, mixed well, and extracted by hot reflux at 80℃ for 3 hours. After extraction, the mixture was coarsely filtered, then filtered through a 220nm filter membrane and the filtrate was collected. The filtrate was concentrated at 50℃ to remove ethanol, thereby obtaining Camellia chrysantha flower extract.

[0026] Macadamia nut oil: purchased from Vantage Specialty Chemicals, Inc.

[0027] Wheat germ oil: purchased from Jiangxi Xinhe Biotechnology Co., Ltd.

[0028] Rice bran oil: purchased from Nanhai Oils & Fats Industry (Chiwan) Co., Ltd.

[0029] Oligopeptide-1, Acetyl Tetrapeptide-3, Tripeptide-1, Hexapeptide-9, Biotin (Runzhisi): Purchased from Runhui Biotechnology Co., Ltd.

[0030] Recombinant type 3 collagen: purchased from Hangzhou Meiliu Biotechnology Co., Ltd.

[0031] Recombinant type 17 collagen: purchased from Zhejiang Weiming Shiguang Biotechnology Co., Ltd.

[0032] Glycolipids: Prepared by Guangzhou Aogu Cosmetics Manufacturing Co., Ltd. The glycolipids are sophorolipids, which are microbial secondary metabolites produced by *Candida albicans* using sugars and vegetable oils as carbon sources through a fermentation process under specific conditions. Fermentation medium: Glucose 100 g / L, yeast extract 5 g / L, KH₂PO₄ 1 g / L, MgSO₄·7H₂O 0.5 g / L, NaCl 0.1 g / L, CaCl₂·2H₂O 0.1 g / L, peptone 0.7 g / L, rapeseed oil 50 g / L, solvent: sterile water. Natural *Candida albicans* fermentation: *Candida albicans* strains (using ATCC 22214 as an example) were selected. The strains were activated by streaking on YPD solid medium / incubating on test tube slants at 30℃ for 48 h to obtain single colonies. Test tube storage: Stored at 4℃ for later use. Pick a single colony from the plate and inoculate it into 50 ml of YPD liquid medium. Incubate at 30°C and 220 rpm for 48 hours on a shaker. After fermentation, raise the temperature of the fermenter to 60°C and maintain it for 20-30 minutes to inactivate the yeast. Separate and collect the brownish-yellow, oily, viscous glycolipid from the bottom. Add an equal volume of warm distilled water (55-65°C) and mix well to wash away any remaining hydrophilic impurities. Reheat (55-65°C) to promote the separation of water and glycolipid. Allow the layers to separate. Collect the lower oily product; the upper aqueous phase can be combined with the bacterial culture layer for further processing. Repeat the washing process 2-3 times to obtain a clear, brownish-yellow, viscous glycolipid.

[0033] Based on the concentration of each component in the composition in Table 1 (based on the weight percentage of the extract solids content in the final prepared solution, with the remainder being deionized water), the corresponding compositions Examples 1-3 and Comparative Examples 1-2 were prepared using conventional mixing methods.

[0034] Table 1

[0035] Comparative Example 3: Compared with Example 1, in the preparation process of this Camellia chrysantha extract, a 40% (v / v) aqueous ethanol solution is added to the Camellia chrysantha powder, and the other preparation methods and formulations are the same as in Example 1.

[0036] Comparative Example 4: Compared with Example 1, in the preparation process of this Camellia chrysantha extract, a 95% ethanol aqueous solution is added to the Camellia chrysantha powder, and other preparation methods and formulations are the same as in Example 1.

[0037] Ultraviolet absorbance test: Ultraviolet absorbance is a measure of a sample’s ability to absorb ultraviolet light of a corresponding wavelength, which characterizes its ultraviolet protection performance. The higher the absorbance, the stronger the sample’s ability to absorb ultraviolet light and the better its protection against ultraviolet light.

[0038] Test method: The prepared sample was diluted 10 times before testing. The quartz cuvette was rinsed three times with the test solution, and the test solution was poured into the quartz cuvette. Pure water was used as a reference, and the absorbance at a wavelength of 275 nm was measured using a spectrophotometer.

[0039] Table 2

[0040] Cytotoxicity and UV-stimulated IL-6 downregulation rate assay: Take the samples from Table 1, dilute them 100 times, and then test them. First, conduct cytotoxicity tests according to the concentrations added in each example and comparative example.

[0041] HaCaT cells in the logarithmic growth phase with approximately 90% confluence were collected and a cell suspension was prepared in a 96-well plate. The solution was then subjected to 10 mJ / cm². 2 Cells were irradiated with UVB and cultured at 37°C and 5% CO2 for 24 hours. A blank control group (BC group), a model group (NC group), and a sample group were set up. The sample group was irradiated with UVB and contained a non-cytotoxic sample bath, the NC group was irradiated with UVB only without a sample bath, and the BC group contained only cell culture medium. After adding samples, the cells were cultured for another 24 hours. The supernatant was collected, and the IL-6 content was determined according to the ELISA kit instructions.

[0042] The results of the cytotoxicity test are shown in Table 3.

[0043] Table 3

[0044] The results of the IL-6 downregulation rate test are shown in Table 4.

[0045] Table 4

[0046] Bliss independence models are widely used in pharmacological research to assess drug combination interactions, particularly in the initial screening and evaluation of potential drug combinations. They are a statistical method for evaluating the effects of multiple drug combinations. The combination index (CI) can be used to evaluate the effectiveness of drug combinations, where CI = E0. 12 / (E1+E2-E1 (E2) CI>1 indicates a synergistic effect, CI=1 indicates an additive effect, and CI<1 indicates an antagonistic effect. The calculated CI=1.26 indicates a synergistic effect in the composition.

[0047] Human skin testing: Using instrumental testing methods, changes in skin elasticity and firmness are measured before and after the subject uses the sample, testing the firming effect of the sample on the human body. The R² value is the skin's viscoelasticity measurement, characterizing the total deformation and recovery of the skin; the F4 value is a skin parameter measured using the negative pressure method, and its value quantifies the degree of skin firmness.

[0048] Prepare solutions according to the concentration ratios in Table 5, using the samples listed in Table 1. Recruit qualified male or female volunteers aged 18-50 as subjects. Ten participants per group will use the corresponding sample. The sample will be applied directly to the head. The first test day will be recorded as D0 (start of use). Follow-up tests will be conducted two weeks after sample use (D14) to measure and record the skin elasticity and firmness of the treated area. The testing instruments will be the Cutometer Dual, Courage & Khazaka skin elasticity probes. A suitable head position should be selected for measurement and application, and this position should not be changed. No sun protection measures should be taken during the sample use period.

[0049] Table 5

[0050] Table 6

[0051] Based on the concentrations of each component in the composition in Table 7 (by weight percentage of the final solution, with the remainder being deionized water), the corresponding compositions in Examples 4-7 were prepared using conventional mixing methods.

[0052] Table 7

[0053] The results of the cytotoxicity test are shown in Table 8.

[0054] Table 8

[0055] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A scalp anti-aging composition with ultraviolet protection function, characterized in that, The scalp anti-aging composition includes Kochia scoparia fruit extract, Solanum nigrum root extract, Acorus calamus root extract and Camellia chrysantha flower extract, wherein the weight ratio of Kochia scoparia fruit extract, Solanum nigrum root extract, Acorus calamus root extract and Camellia chrysantha flower extract is (10-25):(7-56):(15-35):(20-50).

2. The scalp anti-aging composition with UV protection function according to claim 1, characterized in that, The weight ratio of the extracts of Kochia scoparia fruit, Solanum nigrum root, Acorus calamus root and Camellia chrysantha flowers is (20-25):(21-56):(25-35):(25-50).

3. The scalp anti-aging composition with UV protection function according to claim 1, characterized in that, The composition further includes at least one of oils, peptides, collagen, and glycolipids; the oils are selected from macadamia nut oil, wheat germ oil, or rice bran oil; the peptides are selected from one or more of oligopeptide-1, acetyl tetrapeptide-3, tripeptide-1, or hexapeptide-9; and the collagen is selected from recombinant type 3 collagen or recombinant type 17 collagen.

4. The scalp anti-aging composition with UV protection function according to any one of claims 1-3, characterized in that, The preparation method of the Solanum nigrum root extract includes: crushing and sieving dried Solanum nigrum roots, adding pure water at a material-to-liquid ratio of 1:(20-30), ultrasonically extracting at 45-60 ℃ for 2-8 h, centrifuging and filtering to obtain the filtrate.

5. The scalp anti-aging composition with UV protection function according to any one of claims 1-3, characterized in that, The preparation method of the Camellia chrysantha flower extract includes crushing Camellia chrysantha flowers, adding an extraction solvent, refluxing at 70-80 °C, filtering and collecting the extract, and concentrating; the solvent includes an ethanol solution with a mass concentration of 60-70%.

6. The scalp anti-aging composition with UV protection function according to claim 5, characterized in that, The extraction solvent also includes ethoxydiethylene glycol at a mass concentration of 5-15%; the solid-liquid ratio of the extraction solvent is 1:(10-20); the reflux extraction time is 1-4 h.

7. The composition with hair care effect on damaged hair according to claim 5, characterized in that, The extraction solvent is a 65% aqueous solution of ethanol containing 10% ethoxydiethylene glycol.

8. The scalp anti-aging composition with UV protection function according to claim 3, characterized in that, The glycolipids include lactone-type sophorolipids.

9. The use of the scalp anti-aging composition with UV protection function according to claim 1 in the preparation of cosmetics with scalp anti-aging, UV protection or anti-inflammatory effects.

10. The application according to claim 9, characterized in that, The cosmetics include at least one of the following: hair pre-shampoo, shampoo, hair oil, conditioner, hair mask, hair serum, or hair oil.

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