A sensitive skin soothing and lightening composition synergistically enhanced by dendrobium nobile and sphingomonas and application thereof

Abstract

The present application relates to a sensitive skin soothing and brightening composition, its preparation method and application, and belongs to the technical field of cosmetics. The composition of the present application comprises the following components by weight: 4-tert-butylcyclohexanol 0.3-2.0 parts, sphingomonad ferment extract 0.5-5.0 parts, 3-o-ethyl ascorbic acid 1.0-4.0 parts, dendrobium nobile extract 1.0-8.0 parts. The composition of the present application has excellent soothing anti-inflammatory, whitening, thickening skin basement membrane and improving skin stress resistance effects through the synergistic effect of dendrobium nobile and sphingomonad, and is mild and non-irritating, suitable for sensitive skin.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a soothing and brightening composition for sensitive skin that utilizes the synergistic effect of Dendrobium nobile and Sphingosomalis, and its application. Background Technology

[0002] Sensitive skin (SS) is a common skin problem. The main clinical manifestations of sensitive skin are subjective symptoms such as burning, stinging, itching, and tightness after the skin is stimulated by physical, chemical, or psychological factors, with or without objective signs such as erythema, scaling, and telangiectasia. In recent years, with the aggravation of environmental pollution, improper use of cosmetics, and increased psychological stress, the incidence of sensitive skin has been rising year by year, becoming an important issue that urgently needs to be addressed in the cosmetics industry.

[0003] The pathogenesis of sensitive skin is complex, and it is generally believed to be closely related to impaired skin barrier function, neurosensory dysfunction, immune inflammatory response, and increased vascular reactivity. Among these factors, the overactivation of the transient receptor potential vanilloid 1 (TRPV1) channel is considered a core component of neurogenic inflammation in sensitive skin. TRPV1 is a non-selective cation channel widely distributed in sensory nerve endings, keratinocytes, and melanocytes. When exposed to external stimuli such as capsaicin, heat, acidic environments, and ultraviolet radiation, TRPV1 channels open, leading to an increase in Ca²⁺ levels. + Influx of neuropeptides (such as substance P and calcitonin gene-related peptide CGRP) triggers the release of these neuropeptides, which in turn activates immune cells such as mast cells and macrophages, releasing inflammatory factors such as IL-1α, IL-6, TNF-α, and PGE2, forming a vicious cycle of "neurogenic inflammation - barrier disruption - exacerbated inflammation." Therefore, targeting and inhibiting TRPV1 channel activity is a key strategy for soothing sensitive skin.

[0004] Dendrobium nobile Lindl., a perennial herb belonging to the genus Dendrobium in the Orchidaceae family, is a traditional and precious Chinese medicinal herb with the effects of nourishing yin and clearing heat, benefiting the stomach and promoting the production of body fluids. Modern research shows that Dendrobium nobile is rich in active ingredients such as polysaccharides, alkaloids, flavonoids, and phenols, possessing various biological activities including antioxidant, anti-inflammatory, immunomodulatory, and cell proliferation-promoting effects. The effective components of the stem and flower parts of Dendrobium nobile differ significantly: the stem is rich in Dendrobium polysaccharides and alkaloids, exhibiting good moisturizing, repairing, and anti-inflammatory effects; while the flower is rich in flavonoids and volatile oils, possessing strong antioxidant and whitening activities. However, current technologies for the application of Dendrobium nobile in cosmetics mostly focus on simple extraction of single parts, failing to fully utilize the synergistic potential of the stem and flower, and lacking targeted design for the TRPV1 pathway in sensitive skin.

[0005] Sphingomonas is a type of Gram-negative bacterium whose fermentation product extract is rich in glycolipid biosurfactants (such as sophorolipids), extracellular polysaccharides, and various active metabolites. Studies have shown that Sphingomonas fermentation products have good skin barrier repair functions, promoting ceramide synthesis, enhancing the water-locking capacity of the stratum corneum, and possessing certain anti-inflammatory and soothing effects. However, its soothing effect is limited when used alone, and the synergistic mechanism with plant extracts has not yet been fully explored.

[0006] 4-T-Butylcyclohexanol is a known TRPV1 receptor antagonist that blocks Ca²⁺ ions by directly binding to TRPV1 channels. + It flows internally, thus quickly relieving discomfort such as burning and stinging on the skin. However, its anti-inflammatory depth is insufficient when used alone, and it may be irritating at high concentrations.

[0007] 3-O-Ethyl Ascorbic Acid (3-O-Ethyl Ascorbic Acid) is a vitamin C derivative with excellent stability and transdermal absorption. It can inhibit tyrosinase activity and reduce melanin, thus exerting a whitening and brightening effect. It also has some antioxidant and anti-inflammatory properties, but its soothing effect on sensitive skin is relatively weak when used alone.

[0008] Existing technologies for soothing and brightening products targeting sensitive skin often employ a simple layering of single-function ingredients, which has the following shortcomings: First, they lack precise targeting of the TRPV1 channel, resulting in a superficial soothing effect that fails to address the root cause of the neurogenic inflammatory cascade. Second, the synergistic effect of plant extracts and microbial fermentation products is not fully utilized, and there is a lack of scientific optimization in the proportions of the components. Third, the combination of whitening and soothing ingredients is unreasonable, with some whitening ingredients (such as high-concentration arbutin and kojic acid) potentially irritating sensitive skin and possibly exacerbating post-inflammatory hyperpigmentation. Fourth, there is a lack of systematic multi-level validation at the cellular, animal, and clinical levels, leading to a lack of scientific basis for efficacy claims.

[0009] Therefore, developing a composition specifically for sensitive skin that can target and inhibit the TRPV1 channel, achieve synergistic effects between plant extracts and microbial fermentation products, combine soothing and repairing effects with whitening and brightening effects, and has undergone multi-level scientific verification, has significant theoretical and market value. Summary of the Invention

[0010] The purpose of this invention is to overcome the shortcomings of the prior art and provide a soothing and brightening composition for sensitive skin with advantages such as synergistic effect, soothing and anti-inflammatory, whitening and brightening, thickening of the skin basement membrane and improvement of skin stress resistance, as well as its preparation method and application.

[0011] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides a sensitive skin soothing and brightening composition that utilizes the synergistic effect of Dendrobium nobile and Sphingomonas sphingosine monophosphate. The active components of the composition include: 4-tert-butylcyclohexanol, Sphingomonas sphingosine monophosphate fermentation product extract, 3-o-ethyl ascorbic acid, and Dendrobium nobile extract.

[0012] Furthermore, the Dendrobium nobile extract specifically includes Dendrobium nobile stem extract and Dendrobium nobile flower extract.

[0013] Furthermore, the weight ratio of the Dendrobium nobile stem extract to the Dendrobium nobile flower extract is 1:5 - 5:1, preferably 1:2 - 3:1.

[0014] Further, the active components in the composition comprise, by weight: 4-tert-butylcyclohexanol: 0.1 - 2.0 parts; Sphingosomalidone fermentation product extract: 0.5-5.0 parts; 3-o-Ethyl ascorbic acid: 1.0 - 4.0 parts; Dendrobium nobile extract: 1.0 - 8.0 parts.

[0015] Further, the active components in the composition comprise, by weight: 4-tert-butylcyclohexanol: 0.3 - 1.0 parts; Sphingosomalidone fermentation product extract: 1.0 - 3.0 parts; 3-o-Ethyl ascorbic acid: 1.5 - 2.5 parts; Dendrobium nobile extract: 2.0 - 5.0 parts.

[0016] Secondly, the present invention provides a method for preparing the aforementioned sensitive skin soothing and brightening composition, comprising the following steps: S1. Preparation of Dendrobium nobile extract: The dried Dendrobium nobile stems and flowers were mixed and pulverized in proportion, added to 5-10 times the amount of ethanol aqueous solution, and extracted with ultrasonic assistance at 40℃-60℃. The filtrate was filtered and collected, concentrated under reduced pressure and dried under vacuum to obtain Dendrobium nobile extract. S2. Compounding and Dissolving: The Dendrobium nobile extract, 3-o-ethyl ascorbic acid and Sphingomonas fermentation product extract obtained in step S1 are added to a polyol solvent and stirred at 40℃-50℃ until completely dissolved to form premix A. S3, Nerve-soothing component: 4-tert-butylcyclohexanol is heated and melted at 65-75°C, and then slowly added to the premixed liquid A. The mixture is stirred evenly at 300-500 rpm at room temperature to obtain the sensitive skin soothing and brightening composition.

[0017] Furthermore, the polyol solvent is one or more of glycerol, butanediol, propylene glycol, or 1,2-hexanediol.

[0018] Thirdly, the present invention provides a cosmetic comprising any of the aforementioned compositions, wherein the composition comprises 1.0% to 15.0% by mass in the cosmetic, preferably 3.0% to 8.0%.

[0019] Fourthly, the present invention provides the application of the sensitive skin soothing and brightening composition described in any one of the foregoing in the preparation of skin care products for thickening the skin basement membrane, whitening and brightening skin tone, and improving the skin's resistance to external physical or chemical stress, wherein the dosage form of the cosmetic includes lotion, serum, cream, and toner.

[0020] Furthermore, the skincare product whitens and brightens the skin tone by reducing pigmentation.

[0021] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) Innovative Synergistic Mechanism: This invention is the first to scientifically combine Dendrobium nobile (stem-flower synergy) with Sphingomonas fermentation product extract, which produces a significant synergistic effect in soothing and anti-inflammatory effects. Dendrobium nobile extract regulates inflammatory signaling pathways through multiple targets, while Sphingomonas fermentation product repairs the skin barrier and promotes ceramide synthesis. The combined use of the two can form a dual mechanism of "exogenous soothing and endogenous repair", which is more effective than single components.

[0022] (2) Precise targeting of the TRPV1 channel: 4-tert-butylcyclohexanol, as a TRPV1 receptor antagonist, can rapidly block Ca²⁺ induced by stimuli such as capsaicin. + The endogenous flow of the drug disrupts the neurogenic inflammatory cascade at its source; the flavonoids and polysaccharides in Dendrobium nobile extract can further downregulate the expression of TRPV1 channels, achieving precise intervention of "rapid blockade + long-term downregulation".

[0023] (3) Organic unity of soothing and whitening: This invention breaks through the technical bottleneck of traditional sensitive skin products that "emphasize soothing and neglect whitening" or that whitening ingredients stimulate sensitive skin. While gently whitening, 3-o-ethyl ascorbic acid works synergistically with the flavonoids in Dendrobium nobile flower extract to inhibit tyrosinase activity; at the same time, it reduces post-inflammatory hyperpigmentation (PIH) by blocking the TRPV1 channel, thus realizing the innovative concept of "anti-inflammatory equals whitening".

[0024] (4) Thickening the skin basement membrane: The polysaccharide components in the Dendrobium nobile stem extract can promote the synthesis of type IV collagen and laminin, while the glycolipid components in the fermentation products of Sphingosine monocytogenes can promote the differentiation of keratinocytes. The two work together to thicken the skin basement membrane and enhance the structural integrity and stress resistance of the skin.

[0025] (5) Excellent safety and mildness: After multi-level verification through cytotoxicity test (CCK-8 method), animal test and human clinical trial, the composition of the present invention has no significant toxicity to HaCaT cells within the effective concentration range, no irritation to tricolor guinea pigs and human sensitive skin subjects, and the redness improvement rate is significantly better than that of the positive control, which reflects excellent mildness and safety.

[0026] (6) The preparation process is simple and efficient: The present invention adopts an ultrasonic-assisted extraction, low-temperature dissolution and stepwise compounding preparation process, which is simple to operate, mild in conditions, easy to industrialize, and can retain the biological activity of each component to the greatest extent. Attached Figure Description

[0027] Figure 1 HE staining image of the right skin of group 2 on day 28; Figure 2 HE staining image of the left skin of group 2 on day 28. Detailed Implementation

[0028] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0029] The materials used in the following embodiments and comparative examples are from the following sources: In this invention, the complete DMEM culture medium is DMEM culture medium containing 10% FBS. The reagent kits, reagents, cells, and other experimental materials used in this invention are all commercially available products and can be routinely obtained by those skilled in the art.

[0030] Examples 1-5 The components of Examples 1-5 are shown in Table 1.

[0031] The basic raw material preparation methods are as follows: S1. Preparation of Dendrobium nobile extract: Dried Dendrobium nobile stems and flowers were mixed and pulverized in proportion (see Table 1 below for weight ratio), and passed through a 60-mesh sieve. Seven times the volume of 60% ethanol aqueous solution was added, and ultrasonic-assisted extraction was performed at 50℃. The filtrate was filtered and collected, concentrated under reduced pressure, and vacuum dried to obtain Dendrobium nobile extract. (The ultrasonic-assisted extraction conditions were: ultrasonic power: 400 W, ultrasonic frequency: 40 kHz; extraction temperature: 50℃; extraction twice, 60 min each time; fine filtration using a 0.22 μm microporous membrane; the reduced pressure concentration conditions were: vacuum degree: -0.085 MPa ~ -0.095 MPa, water bath temperature: 50℃, concentrated to a thick paste with a relative density of 1.20 (measured at 60℃); the vacuum drying conditions were: vacuum degree ≤ -0.09...) (The powder was dried at 55℃ for 12 hours with a pressure of MPa until constant weight, then pulverized through a 100-mesh sieve to obtain Dendrobium nobile extract powder.) S2. Compounding and Dissolving: The Dendrobium nobile extract, 3-o-ethyl ascorbic acid and Sphingomonas fermentation product extract obtained in step S1 were added to a polyol solvent (see Table 1) and stirred at 45°C until completely dissolved to form premix A. S3, Nerve-soothing component: 4-tert-butylcyclohexanol is heated and melted into a liquid under a water bath at 70°C, and then slowly added to the premixed liquid A. The mixture is stirred evenly at 400 rpm at room temperature to obtain the sensitive skin soothing and brightening composition.

[0032] Polyol solvent (S2): A mixture of glycerol and 1,2-hexanediol in a mass ratio of 3:1 is selected.

[0033] Table 1

[0034] Comparative Examples 1-5 The comparative design uses the single-variable method, and the preparation method is the same as in the previous embodiments. The specific component allocation is as follows: Comparative Example 1: Except for the absence of Dendrobium nobile extract, the other components, proportions and preparation process were exactly the same as in Example 1 (the missing weight parts were made up with polyol solvent, the same below).

[0035] Comparative Example 2: Except for the absence of Sphingomonas fermentation product extract, it was the same as or similar to Example 1.

[0036] Comparative Example 3: Except that the Dendrobium nobile extract was extracted entirely from Dendrobium nobile stems (excluding flowers), the rest was the same or similar to Example 1.

[0037] Comparative Example 4: Except for the fact that the Dendrobium nobile extract was extracted entirely from Dendrobium nobile flowers (excluding stems), the rest was the same or similar to Example 1.

[0038] Comparative Example 5: Extreme component ratio. The components included 3.5 parts by weight of 4-tert-butylcyclohexanol, 0.1 parts by weight of Sphingomonas fermentation product extract, 5.0 parts by weight of 3-o-ethyl ascorbic acid, 0.2 parts by weight of Dendrobium nobile extract (stem:flower ratio) (1:1), and 91.2 parts by weight of polyol solvent (glycerol to 1,2-hexanediol 3:1). The preparation process was the same as or similar to that in Example 1.

[0039] Test Example 1 (Cellular Level Test) 1.1 Selection of experimental cell lines Anti-inflammatory test for sensitive skin: Human keratinocytes (HaCaT) (Shanghai Yaji Biotechnology Co., Ltd.)

[0040] 1.2 Grouping of Cytotoxicity Tests Blank control group: DMEM complete culture medium, cell-free; Negative control group: DMEM complete medium containing polyols, HaCaT cells; wherein, the preparation of polyols is the same as in Example 1, and the amount added is the same as the corresponding sample group. Sample groups: Examples 1-5, Comparative Examples 1-5, with 5 concentration gradients (0.0625%, 0.125%, 0.25%, 0.5%, 1.0%, 2.0%), and the solvent used for preparation was DMEM complete culture medium.

[0041] 1.3 Cytotoxicity assay using the CCK-8 assay The basic steps for the determination are as follows: 1.) HaCaT cells were seeded into 96-well plates (5 × 10³ cells / well) and cultured at 37°C with 5% CO2 for 24 h. 2.) Discard the culture medium and add DMEM complete culture medium (100 μL / well) containing different concentrations of samples, with 6 replicates for each concentration; 3.) Incubate at 37℃ with 5% CO2 for another 24 hours; 4.) Add CCK-8 solution (10 μL / well), incubate at 37℃ with 5% CO2 for 2 h; 5.) Measure the absorbance at 450 nm using an ELISA reader and record the absorbance value (A). 6.) Calculate cell viability: Viability (%) = (Sample A - Blank A) / (Negative A - Blank A) × 100% 7. Calculate CC 50 (Half-maximal cytotoxicity concentration).

[0042] 1.4 Cytotoxicity Results After determining and calculating OD450 using the CCK-8 assay, the cell viability (%) of human keratinocytes (HaCaT) in each example and comparative example at different mass percentage concentrations (w / v, %) is shown in Table 2.

[0043] Table 2. Effects of each component on HaCaT cell viability (%) Negative control (NC) 100.0 100.0 100.0 100.0 100.0 100.0 Example 1 78.5 92.1 98.4 99.1 99.5 100.2 Example 2 82.4 94.3 99.0 99.6 100.1 99.8 Example 3 75.2 90.8 97.9 98.8 99.3 99.7 Example 4 88.7 96.5 99.4 100.1 99.9 100.0 Example 5 65.3 84.6 91.2 96.4 98.2 99.1 Comparative Example 1 80.1 93.4 98.6 99.2 99.7 100.1 Comparative Example 2 81.6 94.0 98.9 99.4 99.6 99.8 Comparative Example 3 79.2 92.7 98.2 99.0 99.4 100.0 Comparative Example 4 78.9 92.5 98.1 98.9 99.3 99.9 Comparative Example 5 52.1 74.8 85.3 92.0 96.1 98.5 Therefore, when the sample concentration is in the range of 0.0625% to 0.5%, the cell viability of Examples 1-4 and Comparative Examples 1-4 is greater than 95% (Example 5 is slightly lower, but still greater than 90%). Cell morphology is normal, with no shedding, rounding, or lysis. This indicates that within this concentration range, the composition has no significant cytotoxicity to skin keratinocytes and is highly safe (generally, concentrations below 90% are considered to have potential cytotoxicity).

[0044] Based on the standard principles for evaluating the in vitro efficacy of cosmetic raw materials and preparations (i.e., selecting a concentration with cell viability greater than 90% and as high as possible for subsequent efficacy testing), 0.5% was ultimately selected as the concentration for subsequent tests.

[0045] 1.5 Grouping for the determination of the soothing effect of inflammatory factors Blank control group: DMEM complete culture medium was added only; Model control group: After pretreatment with DMEM complete medium for 2 hours, capsaicin at a final concentration of 100 μM was added to stimulate modeling for 6 hours; Solvent control group: Pretreated with DMEM complete culture medium containing 0.92% polyol solvent (prepared as in Example 1) for 2 hours, followed by stimulation with capsaicin at a final concentration of 100 μM for 6 hours to induce modeling; Positive control group: Pretreated with DMEM complete medium containing lidocaine (100 μM) for 2 hours, followed by stimulation with capsaicin at a final concentration of 100 μM for 6 hours to induce modeling. Example group: each sample was pretreated with DMEM complete medium containing 0.5% of the samples from Examples 1 to 5 for 2 hours, and then stimulated to form a model with capsaicin at a final concentration of 100 μM for 6 hours. Comparative control group: each sample was pretreated with DMEM complete medium containing 0.5% of comparative control 1 to 5 samples for 2 hours, followed by stimulation with capsaicin at a final concentration of 100 μM for 6 hours to induce modeling.

[0046] 1.6 Determination of the soothing effect on inflammatory factors The basic steps for measuring inflammatory factors using the ELISA method are as follows: 1.) Cell seeding: HaCaT cells were seeded in 6-well plates (2 × 10⁻⁶ cells / well). 5 / well), cultured at 37°C and 5% CO2 for 24 hours; until cells adhered and grew to 80% confluence; 2.) Sample pretreatment: Discard the original culture medium and wash twice with PBS; add DMEM complete culture medium (2 mL / well) containing different samples, wherein: Blank control group and model control group: DMEM complete culture medium only; Solvent control group: DMEM complete culture medium containing 0.92% polyol solvent (the polyol was prepared in the same way as in Example 1); Positive control group: DMEM complete medium containing lidocaine (100 μM); Example group: Examples 1-5 (DMEM complete culture medium containing 0.5% sample); Comparative examples: Comparative examples 1-5 (same as above); Incubate at 37°C and 5% CO2 for 2 hours; 3.) Capsaicin stimulation: Keep the original pretreatment culture medium of each group unchanged. Except for the blank control group, all other groups were given capsaicin stock solution to make the final concentration in the wells 100 μM. After adding, they were placed in a 37°C, 5% CO2 incubator and continued to be cultured for 6 hours. 4.) Collection of supernatant: After stimulation, aspirate the cell culture supernatant from each well into a centrifuge tube and centrifuge at 3000 rpm for 10 minutes at 4°C. Aliquot the supernatant and immediately store it at -80°C for subsequent ELISA detection of IL-1α, IL-6, TNF-α, and PGE2.

[0047] 5.) Cell Lysis and Total Protein Concentration Correction: After collecting the supernatant, each well of cells was carefully washed twice with ice-cold PBS buffer. An appropriate amount of RIPA cell lysis buffer containing PMSF was added to each well, and the cells were incubated on ice for 30 minutes for static lysis, repeatedly pipetting to ensure complete cell lysis. The supernatant was collected after centrifugation (4°C, 12000 rpm, 15 min). The total protein concentration of each well was determined using a BCA protein quantification kit to standardize the corresponding inflammatory factor secretion levels.

[0048] 6.) ELISA assay: Absorbance was measured according to the instructions of the respective ELISA kits for each inflammatory factor. The final expression level of the inflammatory factor was expressed as the concentration per unit protein content, calculated as follows: Inhibition rate (%) = (C 模型 - C 样品 ) / (C 模型 - C 空白 ) × 100% Wherein, C in the formula 模型 C represents the concentration of inflammatory factors detected in the model control group. 样品 C represents the concentration of each test group or comparative group. 空白 The values ​​represent the background concentration for the blank control group. The results are shown in Table 3.

[0049] Table 3. Effects of each component on capsaicin-induced secretion of inflammatory factors in HaCaT cells. Solvent control (VC) 4.3 3.9 3.1 3.8 Positive control (PC) 75.3 77.8 78.0 78.4 Example 1 78.9 81.7 82.5 83.3 Example 2 73.2 75.5 76.0 76.8 Example 3 65.0 66.9 67.8 68.7 Example 4 69.8 72.1 73.0 73.7 Example 5 53.0 54.8 53.9 53.7 Comparative Example 1 43.7 44.5 42.7 44.1 Comparative Example 2 46.6 47.2 46.2 46.6 Comparative Example 3 39.6 40.3 39.8 39.8 Comparative Example 4 41.2 42.3 41.2 41.3 Comparative Example 5 26.4 27.1 24.2 23.8 Therefore, the inhibition rates of various factors in the solvent control group were not significantly different from those in the model control group, proving that the polyol solvent used in this experiment does not possess anti-inflammatory and soothing effects, nor does it interfere with the modeling system. All example groups exhibited extremely strong inhibitory effects on inflammatory factors. Among them, Example 1 showed the most significant effect.

[0050] In summary, the components in the embodiments of the present invention, through scientific formulation, produce a synergistic effect, which can effectively block the downstream inflammatory factor cascade release caused by TRPV1 channel activation, and has excellent effects in soothing sensitive skin, resisting irritation and repairing the skin barrier.

[0051] Test Example 2: Pigment Removal Animal Experiment 2.1 Product Formulation The composition of Example 1 was formulated into a product suitable for use on sensitive skin, as shown in Table 4 below: Table 4 Finished Product Formulation Table Deionized water To 100 Composition 3.0 or 5.0 Xanthan gum 0.2 Glycerin 5.0 Butylene glycol 3.0 Sodium hyaluronate 0.1 Squalane 4.0 Jojoba seed oil 2.0 Caprylic / capric triglyceride 3.0 Potassium cetyl phosphate 1.0 Glyceryl stearate 1.2 p-Hydroxyacetophenone 0.5 Hexylene glycol 0.8 Tocopheryl acetate 0.5 2.2 Evaluation of the skin pigmentation removal efficacy of tricolor guinea pigs: experimental grouping Thirty healthy tricolor guinea pigs (weighing 300-350g, half male and half female) were selected and kept in an environment with a temperature of 22±2℃ and a relative humidity of 50%-60% for one week for acclimatization.

[0052] Back hair removal: 24 hours before the test, use an animal razor to shave the hair on both sides of the guinea pig's back. Select white or brown areas and mark an area of ​​about 3 cm × 3 cm on each side for the test.

[0053] UVB-induced pigmentation model: Pigmentation was induced using ultraviolet light (UVB irradiation wavelength 290~320 nm, peak 311 nm). The bare skin on the backs of guinea pigs was irradiated daily at a dose of 500 mJ / cm². 2 The animals were irradiated continuously for 14 days until visible, uniform skin pigmentation was observed. They were then randomly divided into 6 groups of 5 animals each, as follows: Negative control group: fed normally; Positive control group: The control product with 2% arbutin applied; Test groups 1-2: Products containing 3.0% and 5.0% of the composition of Example 1, respectively, were applied to the product containing the composition of the present invention. Comparative Example 1: Apply the finished product containing 5.0% of the composition of Comparative Example 1; Comparative Example 2: Apply the finished product containing 5.0% of the composition of Comparative Example 5.

[0054] Administration method: Each group of animals was given the corresponding product twice a day, morning and evening (0.2 mL each time), and it was evenly applied to the test area (right side). The left side was given 0.2 mL of physiological saline as a self-control. The skin condition of the left and right sides was selected as much as possible to eliminate the difference between the control and the control. The administration was continued for 28 days.

[0055] 2.3 Evaluation Indicators Skin color brightness value (L*) determination: The L* value (brightness value; the higher the value, the whiter the skin) of the test area was measured using a skin colorimeter (Spectrophotometer CM-25d) before drug administration (day 0), day 14, and day 28. Simultaneously, after the test (day 28), the animals were euthanized, and skin tissue from the test sites was harvested. After fixation, dehydration, and paraffin embedding, HE staining was performed to observe the distribution and density of melanin granules in the basal layer of the epidermis. The results are shown in [Figure number missing]. Figures 1-2 .

[0056] 2.4 Test Results See Table 5.

[0057] Table 5. Results of the effects of each component on skin color in guinea pigs.

[0058] Before administration (day 0), after modeling with the same dose of UVB ultraviolet irradiation, the skin brightness values ​​of all groups were between 42.2 and 42.6. After continuous administration for 28 days at the test substance concentration, the brightening effect of this invention was superior to the positive group; at a 5% concentration, the skin brightness value increased significantly from an extremely dull 42.4 to 60.1. From the effects of the control examples, the whitening and brightening effects were severely limited in the absence of Dendrobium nobile extract. Based on the results of test example 1, it can be inferred that Dendrobium nobile extract effectively blocks the TRPV1 channel, cutting off the continuous activation signal of downstream melanocytes by micro-inflammation. In contrast, due to its strong cellular irritation, control example 2 resulted in secondary post-inflammatory hyperpigmentation induced by chemical substances after UVB stimulation, which hindered the normal clearance of melanin. In summary, the components in Example 1 of this invention have the best efficacy due to their mild, safe, and scientifically formulated composition.

[0059] Test Example 3: Clinical Trial 3.1 Subject Recruitment Inclusion criteria: Age range: 18-35 years old Skin type: Sensitive skin; among which, lactic acid stinging score >3 or sensitive skin questionnaire self-score >21 (Group standard "TGDCA-029 Evaluation of the efficacy of cosmetics suitable for sensitive skin").

[0060] 3.2 Design Grouping Total sample size: 30 eligible participants; Randomized grouping: 6 experimental groups; Group size: 5 people per group.

[0061] Under normal circumstances, volunteers applied the product to their entire face. They took an appropriate amount of lotion, applied it to their face, and massaged it in until absorbed. In an air-conditioned room with a temperature of 21±1℃ and humidity of 50±10%, the improvement in facial indicators was quantified and photographed using instruments. The testing was conducted before use, 1 day after use, and 14 days after use. Skin brightness was measured using the Skin-Glossymeter GL 200 skin analyzer, and skin redness was measured using the Skin-Colorimeter CL400 skin color difference test probe.

[0062] The calculation formula is as follows: Brightness improvement rate = (brightness) t -Brightness 0) / Brightness 0 × 100%; Redness Improvement Rate = (Redness 0 - Redness) t Redness 0×100%.

[0063] 3.3 Test Results Subjects used the corresponding product continuously for 14 days. Instrument tests were performed on day 0 (before use), day 1, and day 14. The results of skin brightness improvement rate and redness improvement rate for each group of subjects are shown in Table 6.

[0064] Table 6. The effect of each product group on improving skin brightness and redness in subjects with sensitive skin (%)

[0065] Note: A negative redness improvement rate indicates that the skin erythema has worsened or that an irritation reaction has occurred. As can be seen from the redness improvement rate in Table 6, Experimental Group 1 (Example 1) showed a significant advantage in repairing and reducing redness. After only one day of use (D1), its redness improvement rate reached 10.6%, significantly higher than other groups; after 14 days of continuous use (D14), the redness improvement rate was as high as 22.4%. It is speculated that this may be due to the synergistic effect of 4-tert-butylcyclohexanol, Dendrobium nobile extract (stem and flower ratio 2:1), and Sphingomonas fermentation product extract, which can quickly block the activation of TRPV1 channels in sensitive skin, cut off the cascade reaction of neurogenic micro-inflammation from the source, and thus show excellent immediate reduction of facial erythema and long-term repair of the skin barrier in clinical practice.

[0066] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A soothing and brightening composition for sensitive skin utilizing the synergistic effect of Dendrobium nobile and Sphingomonas, characterized in that, The active components of the composition include: 4-tert-butylcyclohexanol, Sphingomonas fermentation product extract, 3-o-ethyl ascorbic acid, and Dendrobium nobile extract.

2. The sensitive skin soothing and brightening composition as described in claim 1, characterized in that, The Dendrobium nobile extract specifically includes Dendrobium nobile stem extract and Dendrobium nobile flower extract.

3. The sensitive skin soothing and brightening composition as described in claim 2, characterized in that, The weight ratio of the Dendrobium nobile stem extract to the Dendrobium nobile flower extract is 1:5 - 5:

1.

4. The sensitive skin soothing and brightening composition as described in claim 1, characterized in that, The active components in the composition, by weight, comprise: 4-tert-butylcyclohexanol: 0.1 - 2.0 parts; Sphingosomalidone fermentation product extract: 0.5-5.0 parts; 3-o-Ethyl ascorbic acid: 1.0 - 4.0 parts; Dendrobium nobile extract: 1.0 - 8.0 parts.

5. The sensitive skin soothing and brightening composition as described in claim 4, characterized in that, The active components in the composition, by weight, comprise: 4-tert-butylcyclohexanol: 0.3 - 1.0 parts; Sphingosomalidone fermentation product extract: 1.0 - 3.0 parts; 3-o-Ethyl ascorbic acid: 1.5 - 2.5 parts; Dendrobium nobile extract: 2.0 - 5.0 parts.

6. The method for preparing the sensitive skin soothing and brightening composition as described in claim 1, characterized in that, Includes the following steps: S1. Preparation of Dendrobium nobile extract: The dried Dendrobium nobile stems and flowers were mixed and pulverized in proportion, added to 5-10 times the amount of ethanol aqueous solution, and extracted with ultrasonic assistance at 40℃-60℃. The filtrate was filtered and collected, concentrated under reduced pressure and dried under vacuum to obtain Dendrobium nobile extract. S2. Compounding and Dissolving: The Dendrobium nobile extract, 3-o-ethyl ascorbic acid and Sphingomonas fermentation product extract obtained in step S1 are added to a polyol solvent and stirred at 40℃-50℃ until completely dissolved to form premix A. S3, Nerve-soothing component: 4-tert-butylcyclohexanol is heated and melted at 65-75°C, and then slowly added to the premixed liquid A. The mixture is stirred evenly at 300-500 rpm at room temperature to obtain the sensitive skin soothing and brightening composition.

7. The preparation method according to claim 6, characterized in that, The polyol solvent is a mixture of one or more of glycerol, butanediol, propylene glycol, or 1,2-hexanediol.

8. A cosmetic comprising the composition according to any one of claims 1-5, characterized in that, The composition is present in the cosmetic at a mass percentage of 1.0% to 15.0%, and the cosmetic is in the form of lotion, serum, cream, or toner.

9. The use of the sensitive skin soothing and brightening composition according to any one of claims 1-5 in the preparation of skin care products for thickening the skin basement membrane, whitening and brightening skin tone, and improving the skin's resistance to external physical or chemical stress.

10. The application as described in claim 9, characterized in that, The skincare product brightens and evens out skin tone by reducing pigmentation.

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