Ergothioneine-collagen peptide composition for preventing aging and use thereof

Abstract

This invention relates to the field of biomedical technology, disclosing an ergothioneine-collagen peptide composition for anti-aging and its applications. The ergothioneine-collagen peptide composition comprises the following components in parts by weight: 10-16 parts astaxanthin-fish oil complex microcapsules, 0.02-0.3 parts ergothioneine, 45-65 parts fish collagen peptides, 10-25 parts fructooligosaccharides, 3-10 parts sea cucumber peptides, 0.5-3 parts vitamin C, 3-12 parts nut powder, and 0.1-0.6 parts ferrous fumarate. The ergothioneine-collagen peptide composition for anti-aging described in this invention can effectively scavenge DPPH free radicals, ABTS free radicals, and hydroxyl free radicals, and can improve H2O2-induced cellular oxidative damage, increase cell survival rate, reduce ROS and MDA levels, and increase SOD activity, exhibiting good antioxidant and anti-aging effects. It can be used to prepare anti-aging pharmaceuticals.

Description

Technical Field

[0001] This invention relates to the field of biomedical technology, and in particular to an ergothioneine-collagen peptide composition for preventing aging and its application. Background Technology

[0002] With the fast pace of life, environmental pollution, ultraviolet radiation, unhealthy diets, and increased mental stress, the body is in a state of oxidative stress for extended periods, easily leading to the excessive accumulation of reactive oxygen species (ROS). Excessive free radicals can attack cell membrane lipids, proteins, and nucleic acids, causing lipid peroxidation, impaired mitochondrial function, and decreased cell vitality, further inducing problems such as dull skin, decreased elasticity, collagen loss, and accelerated cellular aging. Therefore, improving the body's antioxidant capacity, reducing cellular oxidative damage, and delaying aging through nutritional intervention has become an important research direction for pharmaceuticals.

[0003] Currently, most common antioxidant or anti-aging products on the market mainly consist of single or simple compound ingredients such as vitamin C, vitamin E, collagen peptides, astaxanthin, fish oil, or plant extracts. While these ingredients have certain free radical scavenging, nutritional supplementation, or skin support effects, their pathways of action are relatively limited, making it difficult to simultaneously cover multiple aspects such as aqueous phase antioxidant activity, lipid phase antioxidant activity, cell membrane protection, mitochondrial protection, collagen synthesis support, and nutrient absorption improvement. For example, vitamin C mainly functions in the aqueous phase, astaxanthin mainly functions in the lipid phase and cell membrane environment, and collagen peptides mainly provide the nutritional basis for collagen synthesis and tissue repair. These ingredients cannot form a continuous and comprehensive antioxidant and anti-aging protection system.

[0004] Furthermore, some fat-soluble antioxidants, such as astaxanthin, have poor stability and are easily degraded by factors such as light, heat, and oxygen. Direct addition also presents problems such as poor dispersibility and insufficient absorption and utilization. Fish oil is rich in unsaturated fatty acids, which have the potential to improve cell membrane nutrition, but it is also easily oxidized. Improper combination with other antioxidants may affect product stability and actual efficacy. Therefore, improving the stability, dispersibility, and bioavailability of astaxanthin and fish oil, and enabling it to form a multi-pathway synergistic antioxidant system with components such as ergothioneine, collagen peptides, sea cucumber peptides, and vitamin C, is a crucial technical problem that urgently needs to be solved in the development of anti-aging drugs.

[0005] Based on this, the present invention provides a well-formulated and stable anti-aging composition that can effectively scavenge free radicals, improve H2O2-induced cellular oxidative damage, increase cell survival rate, reduce ROS and MDA levels, and increase SOD activity, thereby achieving good antioxidant and anti-aging effects. Summary of the Invention

[0006] The purpose of this invention is to provide an ergothioneine-collagen peptide composition for preventing aging and its application. This composition can effectively scavenge DPPH free radicals, ABTS free radicals and hydroxyl free radicals, improve H2O2-induced cellular oxidative damage, increase cell survival rate, reduce ROS and MDA levels, and increase SOD activity. It has good antioxidant and anti-aging effects and can be used to prepare drugs for preventing aging.

[0007] To achieve the above objectives, in a first aspect, the present invention provides an ergothioneine-collagen peptide composition for preventing aging, comprising the following components in parts by weight: 10-16 parts astaxanthin-fish oil complex microcapsules, 0.02-0.3 parts ergothioneine, 45-65 parts fish collagen peptides, 10-25 parts fructooligosaccharides, 3-10 parts sea cucumber peptides, 0.5-3 parts vitamin C, 3-12 parts nut powder, and 0.1-0.6 parts ferrous fumarate.

[0008] Furthermore, in the astaxanthin-fish oil composite microcapsules, the fish oil content is 40%-50% by mass, and the astaxanthin content is 0.2%-0.3% by mass.

[0009] Furthermore, the nut powder is one or more of cashew powder or walnut powder, with a particle size of 40-80μm.

[0010] Furthermore, the preparation method of the astaxanthin-fish oil composite microcapsules is as follows: Step 1, Preparation of the composite oil phase: Weigh out fish oil and astaxanthin oleoresin, add astaxanthin oleoresin to fish oil, then add antioxidants, stir and mix under light-protected conditions, control the temperature at 40-50℃, and stir for 20-30 minutes to fully disperse astaxanthin oleoresin in fish oil, and obtain astaxanthin-fish oil composite oil phase. Step 2, Preparation of the aqueous phase of the wall material: Add the wall material to purified water and stir to dissolve it at 40-60℃. Hydrate for 60-90 minutes to obtain the wall material hydrated solution. Then add lecithin to the wall material hydrated solution and continue stirring for 10-30 minutes to ensure the emulsifier is evenly dispersed, thus obtaining the wall material aqueous phase. Step 3, preparation of colostrum: Under stirring conditions, the astaxanthin-fish oil composite oil phase obtained in step 1 was slowly added to the wall material aqueous phase obtained in step 2, and then high-speed shear emulsification was performed. The shearing speed was 10,000-12,000 rpm and the shearing time was 8-15 minutes to obtain astaxanthin-fish oil crude emulsion. Step 4, High-pressure homogenization: The astaxanthin-fish oil crude emulsion obtained in step 3 was subjected to high-pressure homogenization at a pressure of 30-50 MPa and for 2 times to obtain an astaxanthin-fish oil composite emulsion. Step 5, spray drying: The astaxanthin-fish oil composite emulsion obtained in step 4 was sent to a spray drying device for spray drying. The inlet air temperature was controlled at 150-170℃ and the outlet air temperature at 75-85℃ to obtain astaxanthin-fish oil composite microcapsules.

[0011] Further, in step 1, the antioxidant is one or more of vitamin E, ascorbyl palmitate, and rosemary extract; the mass ratio of fish oil: astaxanthin oleoresin: vitamin E is 45:2.5:0.3.

[0012] Furthermore, in step 2, the wall material is composed of maltodextrin, gum arabic, and sodium octenyl succinate starch, with a mass ratio of maltodextrin: gum arabic: sodium octenyl succinate starch: lecithin of 30:12:7:3; the solid content of the aqueous phase of the wall material is 20%-30%.

[0013] Furthermore, in step 4, after homogenization, the average particle size of the astaxanthin-fish oil composite emulsion is 0.5-1.0 μm.

[0014] Secondly, the present invention provides a method for preparing the above-mentioned anti-aging ergothioneine-collagen peptide composition, comprising the following steps: Step S1, Raw material pretreatment: Astaxanthin-fish oil complex microcapsules, ergothioneine, fish collagen peptides, fructooligosaccharides, sea cucumber peptides, vitamin C, nut powder, and ferrous fumarate are prepared; fish collagen peptides, fructooligosaccharides, sea cucumber peptides, vitamin C, nut powder, and ferrous fumarate are each passed through a 60-mesh sieve and set aside. Step S2, premixing of trace components: Ergothioneine, vitamin C, and ferrous fumarate are mixed with 1 / 2 the weight of fructooligosaccharides for 5-15 minutes to obtain a trace component premix. Step S3, mixing of peptide base materials: Mix fish collagen peptides, sea cucumber peptides and the remaining fructooligosaccharides for 10-20 minutes to obtain peptide base material; Step S4, Total Mixing: Add the trace component premix obtained in step S2 to the peptide base obtained in step S3 and continue mixing for 10-20 minutes; then add nut powder and astaxanthin-fish oil composite microcapsules and mix for 5-15 minutes to obtain an anti-aging ergothioneine-collagen peptide composition. Step S5, Post-processing and Packaging: The composition obtained in step S4 is subjected to quality testing. Products that pass the test are packaged to obtain the finished product.

[0015] Furthermore, the finished product is in the form of granules, tablets, solid beverages, capsules, or pills.

[0016] Thirdly, the present invention provides the application of the above-mentioned anti-aging ergothioneine-collagen peptide composition in the preparation of antioxidant pharmaceuticals.

[0017] The advantages and positive effects of the anti-aging ergothioneine-collagen peptide composition and its application described in this invention are as follows: 1. The fish collagen peptides and sea cucumber peptides in this application are rich in small molecule active peptides and a variety of amino acids, which can directly participate in the scavenging of free radicals and provide a nutritional basis for cell repair, collagen synthesis and extracellular matrix stabilization. When used together, the two can supplement peptide active ingredients from different sources and enhance the protective effect against oxidative damage to cells.

[0018] 2. Ergothionein in this application has a strong effect in scavenging reactive oxygen species and protecting mitochondrial function, which can reduce the impact of oxidative stress on cellular energy metabolism. Astaxanthin is a fat-soluble antioxidant that can inhibit lipid peroxidation in lipid-phase environments such as cell membranes; the unsaturated fatty acids in fish oil help improve cell membrane fluidity and nutritional status, and provide a suitable lipid-phase carrier for astaxanthin. After microencapsulation, the stability and utilization rate of both are improved, thereby enhancing lipid-phase antioxidant protection.

[0019] 3. In this application, vitamin C, as a water-soluble antioxidant, can scavenge free radicals and reduce ROS accumulation in an aqueous environment. It can also promote collagen synthesis and help maintain or regenerate the activity of other antioxidant components.

[0020] 4. In this application, fructooligosaccharides improve the gut microbiota and promote the absorption of nutrients; nut powder provides plant protein, unsaturated fatty acids, vitamin E and minerals, which, together with fish oil, astaxanthin and peptides, improve cellular nutrition and the antioxidant environment.

[0021] Therefore, the ergothioneine-collagen peptide composition of the present invention achieves synergistic effects such as antioxidant activity in both aqueous and lipid phases, direct scavenging of free radicals, and collagen support, and has good antioxidant and anti-aging effects, and can be used to prepare drugs for preventing aging.

[0022] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0023] Figure 1 This invention relates to an H2O2-induced oxidative damage model of human skin fibroblasts, and examines the effects of various compositions on cell viability. Figure 2This invention illustrates the effect of each composition on the relative level of intracellular ROS in a H2O2-induced oxidative damage model of human skin fibroblasts. Figure 3 This invention relates to the effects of various compositions on intracellular SOD activity in a H2O2-induced oxidative damage model of human skin fibroblasts. Figure 4 This invention relates to the effects of various compositions on intracellular MDA content in a H2O2-induced oxidative damage model of human skin fibroblasts. Detailed Implementation

[0024] The technical solution of the present invention will be further described below with reference to the accompanying drawings and embodiments.

[0025] Unless otherwise defined, the technical or scientific terms used in this invention shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains.

[0026] Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention. Experimental methods in the following embodiments that do not specify specific conditions are generally determined according to national standards. Experimental instruments, equipment, and reagents in the following embodiments that do not specify their sources are all commercially available materials.

[0027] Unless otherwise defined or stated, all technical and scientific terms used in this invention have the same meaning as those skilled in the art. Furthermore, any methods and materials similar to or equivalent to those described herein may be applied to the methods of this invention.

[0028] The astaxanthin-fish oil complex microcapsules, ergothioneine, fish collagen peptides, fructooligosaccharides, sea cucumber peptides, vitamin C, nut powder, and ferrous fumarate in this application are all food-grade. The astaxanthin oleoresin specification contains 10% astaxanthin.

[0029] The preparation method of the astaxanthin-fish oil composite microcapsules used in this application embodiment is as follows: Step 1, Preparation of the composite oil phase: Weigh out fish oil and astaxanthin oleoresin, add the astaxanthin oleoresin to the fish oil, then add vitamin E, stir and mix under light-protected conditions, control the temperature at 40-50℃, and stir for 20-30 minutes to ensure that the astaxanthin oleoresin is fully dispersed in the fish oil, thus obtaining an astaxanthin-fish oil composite oil phase; wherein, the mass ratio of fish oil:astaxanthin oleoresin:vitamin E is 45:2.5:0.3.

[0030] Step 2, Preparation of the aqueous phase of the wall material: Add the wall material to purified water and stir to dissolve it at 40-60℃. Hydrate for 60-90 minutes to obtain the wall material hydrated solution. Then add lecithin to the wall material hydrated solution and continue stirring for 10-30 minutes to ensure the emulsifier is evenly dispersed, thus obtaining the wall material aqueous phase. The wall material is composed of maltodextrin, gum arabic, and sodium octenyl succinate starch. The mass ratio of the wall material to lecithin is maltodextrin: gum arabic: sodium octenyl succinate starch: lecithin = 30:12:7:3. The solid content of the aqueous phase of the wall material is 20%-30%.

[0031] Step 3, preparation of colostrum: Under stirring conditions, the astaxanthin-fish oil composite oil phase obtained in step 1 was slowly added to the wall material aqueous phase obtained in step 2, and then high-speed shear emulsification was performed. The shearing speed was 10,000-12,000 rpm and the shearing time was 8-15 minutes to obtain astaxanthin-fish oil crude emulsion. Step 4, High-pressure homogenization: The astaxanthin-fish oil crude emulsion obtained in step 3 was subjected to high-pressure homogenization at a pressure of 30-50 MPa for two cycles to obtain an astaxanthin-fish oil composite emulsion. After homogenization, the average particle size of the astaxanthin-fish oil composite emulsion was 0.5-1.0 μm.

[0032] Step 5, spray drying: The astaxanthin-fish oil composite emulsion obtained in step 4 was sent to a spray drying device for spray drying. The inlet air temperature was controlled at 150-170℃ and the outlet air temperature at 75-85℃ to obtain astaxanthin-fish oil composite microcapsules.

[0033] The astaxanthin-fish oil complex microcapsules contain approximately 45% fish oil by mass and approximately 0.25% pure astaxanthin by mass.

[0034] Example 1 An anti-aging ergothioneine-collagen peptide composition comprises the following components in parts by weight: 10 parts astaxanthin-fish oil complex microcapsules, 0.02 parts ergothioneine, 45 parts fish collagen peptides, 10 parts fructooligosaccharides, 3 parts sea cucumber peptides, 0.5 parts vitamin C, 3 parts nut powder, and 0.1 parts ferrous fumarate.

[0035] The preparation method is as follows: Step S1, Raw material pretreatment: Astaxanthin-fish oil complex microcapsules, ergothioneine, fish collagen peptides, fructooligosaccharides, sea cucumber peptides, vitamin C, nut powder, and ferrous fumarate are prepared; fish collagen peptides, fructooligosaccharides, sea cucumber peptides, vitamin C, nut powder, and ferrous fumarate are each passed through a 60-mesh sieve and set aside. Step S2, premixing of trace components: Ergothioneine, vitamin C, and ferrous fumarate were mixed with 1 / 2 the weight of fructooligosaccharides for 10 minutes to obtain a trace component premix. Step S3, mixing of peptide base materials: Fish collagen peptides, sea cucumber peptides and the remaining fructooligosaccharides were mixed for 15 minutes to obtain peptide base material; Step S4, Total Mixing: Add the trace component premix obtained in step S2 to the peptide base obtained in step S3 and continue mixing for 15 minutes; then add nut powder and astaxanthin-fish oil composite microcapsules and mix for 15 minutes to obtain an anti-aging ergothioneine-collagen peptide composition. Step S5, Post-processing and Packaging: The composition obtained in step S4 is subjected to quality testing. Products that pass the test are packaged to obtain the finished product (granules).

[0036] Example 2 An anti-aging ergothioneine-collagen peptide composition comprises the following components in parts by weight: 12 parts astaxanthin-fish oil complex microcapsules, 0.1 parts ergothioneine, 60 parts fish collagen peptides, 12 parts fructooligosaccharides, 5 parts sea cucumber peptides, 1 part vitamin C, 5 parts nut powder, and 0.2 parts ferrous fumarate.

[0037] The preparation method is as follows: Step S1, Raw material pretreatment: Astaxanthin-fish oil complex microcapsules, ergothioneine, fish collagen peptides, fructooligosaccharides, sea cucumber peptides, vitamin C, nut powder, and ferrous fumarate are prepared; fish collagen peptides, fructooligosaccharides, sea cucumber peptides, vitamin C, nut powder, and ferrous fumarate are each passed through a 60-mesh sieve and set aside. Step S2, premixing of trace components: Ergothioneine, vitamin C, and ferrous fumarate were mixed with 1 / 2 the weight of fructooligosaccharides for 10 minutes to obtain a trace component premix. Step S3, mixing of peptide base materials: Fish collagen peptides, sea cucumber peptides and the remaining fructooligosaccharides were mixed for 15 minutes to obtain peptide base material; Step S4, Total Mixing: Add the trace component premix obtained in step S2 to the peptide base obtained in step S3 and continue mixing for 15 minutes; then add nut powder and astaxanthin-fish oil composite microcapsules and mix for 15 minutes to obtain an anti-aging ergothioneine-collagen peptide composition. Step S5, Post-processing and Packaging: The composition obtained in step S4 is subjected to quality testing. Products that pass the test are packaged to obtain the finished product (granules).

[0038] Example 3 An anti-aging ergothioneine-collagen peptide composition comprises the following components in parts by weight: 16 parts astaxanthin-fish oil complex microcapsules, 0.2 parts ergothioneine, 65 parts fish collagen peptides, 18 parts fructooligosaccharides, 7 parts sea cucumber peptides, 1.5 parts vitamin C, 8 parts nut powder, and 0.3 parts ferrous fumarate.

[0039] The preparation method is as follows: Step S1, Raw material pretreatment: Astaxanthin-fish oil complex microcapsules, ergothioneine, fish collagen peptides, fructooligosaccharides, sea cucumber peptides, vitamin C, nut powder, and ferrous fumarate are prepared; fish collagen peptides, fructooligosaccharides, sea cucumber peptides, vitamin C, nut powder, and ferrous fumarate are each passed through a 60-mesh sieve and set aside. Step S2, premixing of trace components: Ergothioneine, vitamin C, and ferrous fumarate were mixed with 1 / 2 the weight of fructooligosaccharides for 10 minutes to obtain a trace component premix. Step S3, mixing of peptide base materials: Fish collagen peptides, sea cucumber peptides and the remaining fructooligosaccharides were mixed for 15 minutes to obtain peptide base material; Step S4, Total Mixing: Add the trace component premix obtained in step S2 to the peptide base obtained in step S3 and continue mixing for 15 minutes; then add nut powder and astaxanthin-fish oil composite microcapsules and mix for 15 minutes to obtain an anti-aging ergothioneine-collagen peptide composition. Step S5, Post-processing and Packaging: The composition obtained in step S4 is subjected to quality testing. Products that pass the test are packaged to obtain the finished product (granules).

[0040] Comparative Example 1 Compared with Example 2, the difference is that ergothioneine is not added, and an equal amount of fructooligosaccharides is used to make up the difference, that is, fructooligosaccharides are 12.1 parts, while the other components and preparation methods are the same as in Example 2.

[0041] Comparative Example 2 Compared with Example 2, the difference is that: astaxanthin-fish oil complex microcapsules are not added, and an equal amount of fructooligosaccharides is added to make up the difference, that is, fructooligosaccharides are 24 parts, and the remaining components and preparation methods are the same as in Example 2.

[0042] Comparative Example 3 Compared with Example 2, the difference is that: no astaxanthin-fish oil composite microcapsules were added, and 5.41 parts of unencapsulated fish oil, 0.30 parts of astaxanthin oleoresin and 0.036 parts of vitamin E with the same active content were used instead, and the total weight was made up with fructooligosaccharides, that is, 18.254 parts of fructooligosaccharides. The remaining components and preparation methods are the same as in Example 2.

[0043] Comparative Example 4 The difference compared to Example 2 is that no sea cucumber peptides were added, and an equal amount of fructooligosaccharides was used to make up the difference.

[0044] Comparative Example 5 The difference from Example 2 is that vitamin C is not added and is supplemented with an equal amount of fructooligosaccharides.

[0045] Experimental Example 1 To evaluate the antioxidant capacity of the compositions of the present invention, the compositions obtained in Examples 1-3 and Comparative Examples 1-5 were tested for DPPH radical scavenging capacity, ABTS radical scavenging capacity and hydroxyl radical scavenging capacity.

[0046] 1. DPPH free radical scavenging ability test: Prepare a 0.1 mmol / L DPPH ethanol solution using anhydrous ethanol and store it in the dark for later use. Prepare 4.0 mg / mL sample solutions using 50% ethanol aqueous solution for each of the compositions obtained in Examples 1-3 and Comparative Examples 1-5. Disperse the solutions ultrasonically for 10 min, centrifuge, and collect the supernatant. Mix 2.0 mL of the sample solution with 2.0 mL of the DPPH ethanol solution, incubate at room temperature in the dark for 30 min, and measure the absorbance at 517 nm, denoted as A1. A blank group and a sample blank control group were also prepared. The blank group consisted of 2.0 mL of DPPH ethanol solution mixed with 2.0 mL of 50% ethanol aqueous solution, and the absorbance was denoted as A0. The sample blank control group consisted of 2.0 mL of sample solution mixed with 2.0 mL of anhydrous ethanol, and the absorbance was denoted as A2. Each group was measured in triplicate, and the average value was taken. DPPH free radical scavenging rate = [1 - (A1 - A2) / A0] × 100%.

[0047] 2. ABTS free radical scavenging ability test: Prepare 7 mmol / L ABTS solution and 2.45 mmol / L potassium persulfate solution, mix equal volumes of the two solutions, and incubate in the dark for 12–16 h to obtain ABTS free radical cation stock solution. Before use, dilute with PBS buffer to obtain an absorbance of 0.70 ± 0.02 at 734 nm to obtain ABTS working solution.

[0048] Take the compositions obtained in Examples 1-3 and Comparative Examples 1-5 respectively, and prepare sample solutions of 2.0 mg / mL with 50% ethanol aqueous solution. After ultrasonic dispersion for 10 min, centrifuge and collect the supernatant for later use. Mix 3.9 mL of ABTS working solution with 0.1 mL of sample solution, react at room temperature in the dark for 6 min, and measure the absorbance at a wavelength of 734 nm. A blank group and a sample blank control group were also set up. Each group was measured in triplicate, and the average value was taken. ABTS free radical scavenging rate = [1-(A 样品 -A 样品空白对照 ) / A 空白 ]×100%.

[0049] 3. Hydroxyl radical scavenging ability test: The salicylic acid method was used to determine the scavenging ability of the samples against hydroxyl radicals. The compositions obtained in Examples 1-3 and Comparative Examples 1-5 were prepared into sample solutions of 4.0 mg / mL using 50% ethanol aqueous solution. After ultrasonic dispersion for 10 min, the solutions were centrifuged, and the supernatant was collected. 1.0 mL of 9 mmol / L FeSO4 solution, 1.0 mL of 9 mmol / L salicylic acid-ethanol solution, 1.0 mL of the sample solution, and 1.0 mL of 8.8 mmol / L H2O2 solution were added sequentially. After mixing, the mixture was reacted in a water bath at 37℃ for 30 min, and the absorbance was measured at 510 nm. A blank group and a sample blank control group were also set up. Each group was measured in triplicate, and the average value was taken. Hydroxyl radical scavenging rate = [1-(A...] 样品 -A 空白对照 ) / A 空白 ]×100%.

[0050] The results are shown in Table 1.

[0051] Table 1. Free radical scavenging ability test results of Examples 1-3 and Comparative Examples 1-5

[0052] As shown in Table 1, Examples 1-3 all exhibited good scavenging effects against DPPH, ABTS, and hydroxyl radicals, with Examples 2 and 3 demonstrating superior free radical scavenging abilities compared to the comparative examples. Compared to Example 2, Comparative Example 1, without the addition of ergothioneine, showed a significant decrease in the scavenging rates of all free radicals, indicating that ergothioneine can enhance the antioxidant capacity of the composition. Comparative Example 2, without the addition of astaxanthin-fish oil composite microcapsules, showed a more significant decrease in free radical scavenging ability, indicating that the astaxanthin-fish oil composite microcapsules are an important component for improving the antioxidant effect of the composition. Comparative Example 3, using unencapsulated astaxanthin oleoresin and fish oil instead of the astaxanthin-fish oil composite microcapsules, showed a lower free radical scavenging ability than Example 2, indicating that microencapsulation improves the stability and dispersibility of astaxanthin and fish oil, thereby enhancing the antioxidant effect of the composition. Comparative Example 4, without the addition of sea cucumber peptides, and Comparative Example 5, without the addition of vitamin C, also showed varying degrees of decrease in free radical scavenging ability, indicating that sea cucumber peptides and vitamin C have a synergistic effect with other components.

[0053] Experimental Example 2 Cellular antioxidant damage and anti-aging effects test: To further evaluate the anti-aging effect of the compositions of the present invention, a human skin fibroblast oxidative damage model induced by H2O2 was used to test the cell protection effect of the compositions obtained in Examples 1-3 and Comparative Examples 1-5.

[0054] Human skin fibroblasts in logarithmic growth phase were seeded in 96-well plates and cultured at 37℃ and 5% CO2 until cell adhesion. The experiment included a blank control group, a model group, Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, and Comparative Example 5. Except for the blank control group and the model group, the corresponding sample solutions were added to each group to a final concentration of 100 μg / mL for 24 h of pretreatment. Subsequently, except for the blank control group, the remaining groups were treated with 200 μmol / L H2O2 for 2 h to establish a cell oxidative damage model. After treatment, cell viability was detected using the CCK-8 assay, intracellular ROS levels were detected using the DCFH-DA fluorescent probe method, and SOD activity and MDA content were detected using a kit method (purchased from Beijing Solarbio Science & Technology Co., Ltd.). Each group was measured in triplicate, and the average value was taken. The test results are shown below. Figures 1-4 As shown.

[0055] Depend on Figures 1-4It can be seen that, compared with the blank control group, the cell survival rate of the model group was significantly decreased, the relative level of ROS and the content of MDA were significantly increased, and the SOD activity was significantly decreased, indicating that H2O2 successfully induced oxidative damage in cells. Compared with the model group, Examples 1-3 all improved cell survival rate, reduced intracellular ROS level and MDA content, and increased SOD activity, indicating that the composition of the present invention can alleviate oxidative stress damage and has a good cell protection effect. Compared with Example 2, the cell protection effect of Comparative Examples 1-5 was reduced to varying degrees. Among them, Comparative Example 1 did not contain ergothioneine, Comparative Example 2 did not contain astaxanthin-fish oil composite microcapsules, and Comparative Example 3, after replacing the astaxanthin-fish oil composite microcapsules with unencapsulated astaxanthin oleoresin and fish oil, had lower cell survival rate and SOD activity than Example 2, and higher ROS level and MDA content than Example 2, indicating that ergothioneine, astaxanthin-fish oil composite microcapsules and their microencapsulation play an important role in improving the antioxidant and anti-aging effects of the composition. The test results of Comparative Examples 4 and 5 show that sea cucumber peptides and vitamin C can also work synergistically with fish collagen peptides, ergothioneine and astaxanthin-fish oil complex microcapsules to exert antioxidant and cell aging delay effects.

[0056] In summary, the composition of this invention exhibits strong scavenging effects against DPPH free radicals, ABTS free radicals, and hydroxyl free radicals, with the scavenging rates of Examples 2 and 3 being significantly better than those of the comparative examples. Cellular experiments showed that this composition can improve the survival rate of cells damaged by H2O2-induced oxidative stress, reduce intracellular ROS levels and MDA content, and increase SOD activity, indicating that it can effectively alleviate oxidative stress damage and has good antioxidant and anti-aging effects.

[0057] Therefore, the anti-aging ergothioneine-collagen peptide composition of the present invention can effectively scavenge DPPH free radicals, ABTS free radicals and hydroxyl free radicals, improve H2O2-induced cellular oxidative damage, increase cell survival rate, reduce ROS and MDA levels, and increase SOD activity. It has good antioxidant and anti-aging effects and can be used to prepare anti-aging drugs.

[0058] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. An anti-aging ergothioneine-collagen peptide composition, characterized in that, It includes the following components in parts by weight: 10-16 parts astaxanthin-fish oil complex microcapsules, 0.02-0.3 parts ergothioneine, 45-65 parts fish collagen peptides, 10-25 parts fructooligosaccharides, 3-10 parts sea cucumber peptides, 0.5-3 parts vitamin C, 3-12 parts nut powder, and 0.1-0.6 parts ferrous fumarate; The astaxanthin-fish oil composite microcapsules contain 40%-50% fish oil and 0.2%-0.3% astaxanthin.

2. The anti-aging ergothioneine-collagen peptide composition according to claim 1, characterized in that: The nut powder is one or more of cashew powder or walnut powder, with a particle size of 40-80μm.

3. The anti-aging ergothioneine-collagen peptide composition according to claim 1, characterized in that, The preparation method of the astaxanthin-fish oil composite microcapsules is as follows: Step 1, Preparation of the composite oil phase: Weigh out fish oil and astaxanthin oleoresin, add astaxanthin oleoresin to fish oil, then add antioxidants, stir and mix under light-protected conditions, control the temperature at 40-50℃, and stir for 20-30 minutes to fully disperse astaxanthin oleoresin in fish oil, and obtain astaxanthin-fish oil composite oil phase. Step 2, Preparation of the aqueous phase of the wall material: Add the wall material to purified water and stir to dissolve it at 40-60℃. Hydrate for 60-90 minutes to obtain the wall material hydrated solution. Then add lecithin to the wall material hydrated solution and continue stirring for 10-30 minutes to ensure the emulsifier is evenly dispersed, thus obtaining the wall material aqueous phase. Step 3, preparation of colostrum: Under stirring conditions, the astaxanthin-fish oil composite oil phase obtained in step 1 was slowly added to the wall material aqueous phase obtained in step 2, and then high-speed shear emulsification was performed. The shearing speed was 10,000-12,000 rpm and the shearing time was 8-15 minutes to obtain astaxanthin-fish oil crude emulsion. Step 4, High-pressure homogenization: The astaxanthin-fish oil crude emulsion obtained in step 3 was subjected to high-pressure homogenization at a pressure of 30-50 MPa and for 2 times to obtain an astaxanthin-fish oil composite emulsion. Step 5, spray drying: The astaxanthin-fish oil composite emulsion obtained in step 4 was sent to a spray drying device for spray drying. The inlet air temperature was controlled at 150-170℃ and the outlet air temperature at 75-85℃ to obtain astaxanthin-fish oil composite microcapsules.

4. The anti-aging ergothioneine-collagen peptide composition according to claim 3, characterized in that: In step 1, the antioxidant is one or more of vitamin E, ascorbyl palmitate, and rosemary extract; the mass ratio of fish oil: astaxanthin oleoresin: vitamin E is 45:2.5:0.

3.

5. The anti-aging ergothioneine-collagen peptide composition according to claim 3, characterized in that: In step 2, the wall material is composed of maltodextrin, gum arabic, and sodium octenyl succinate starch, with a mass ratio of maltodextrin: gum arabic: sodium octenyl succinate starch: lecithin of 30:12:7:3; the solid content of the aqueous phase of the wall material is 20%-30%.

6. The anti-aging ergothioneine-collagen peptide composition according to claim 3, characterized in that: In step 4, after homogenization, the average particle size of the astaxanthin-fish oil composite emulsion is 0.5-1.0 μm.

7. A method for preparing the anti-aging ergothioneine-collagen peptide composition according to any one of claims 1-6, characterized in that, Includes the following steps: Step S1, Raw material pretreatment: Astaxanthin-fish oil complex microcapsules, ergothioneine, fish collagen peptides, fructooligosaccharides, sea cucumber peptides, vitamin C, nut powder, and ferrous fumarate are prepared; fish collagen peptides, fructooligosaccharides, sea cucumber peptides, vitamin C, nut powder, and ferrous fumarate are each passed through a 60-mesh sieve and set aside. Step S2, premixing of trace components: Ergothioneine, vitamin C, and ferrous fumarate are mixed with 1 / 2 the weight of fructooligosaccharides for 5-15 minutes to obtain a trace component premix. Step S3, mixing of peptide base materials: Mix fish collagen peptides, sea cucumber peptides and the remaining fructooligosaccharides for 10-20 minutes to obtain peptide base material; Step S4, Total Mixing: Add the trace component premix obtained in step S2 to the peptide base obtained in step S3 and continue mixing for 10-20 minutes; then add nut powder and astaxanthin-fish oil composite microcapsules and mix for 5-15 minutes to obtain an anti-aging ergothioneine-collagen peptide composition. Step S5, Post-processing and Packaging: The composition obtained in step S4 is subjected to quality testing. Products that pass the test are packaged to obtain the finished product.

8. The preparation method according to claim 7, characterized in that: The finished product is available in the form of granules, tablets, solid beverages, capsules, or pills.

9. The use of the anti-aging ergothioneine-collagen peptide composition according to any one of claims 1-6 in the preparation of antioxidant pharmaceuticals.

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